LOCUS       DAFEZB010000001       131256 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFEZB010000001
VERSION     DAFEZB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 131256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 131256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..131256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.001"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            212..604
                     /locus_tag="LP859_000001"
     CDS             212..604
                     /locus_tag="LP859_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3027992.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LP859_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LP859_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL3027993.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LP859_000003"
     CDS             3165..4775
                     /locus_tag="LP859_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL3027994.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LP859_000004"
     CDS             5067..5936
                     /locus_tag="LP859_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL3027995.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LP859_000005"
     CDS             6101..7372
                     /locus_tag="LP859_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL3027996.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LP859_000006"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LP859_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL3027997.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LP859_000007"
     CDS             8763..9026
                     /locus_tag="LP859_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL3027998.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LP859_000008"
     CDS             9471..9920
                     /locus_tag="LP859_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3027999.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LP859_000009"
     CDS             9977..11395
                     /locus_tag="LP859_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3028000.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LP859_000010"
     CDS             11395..12255
                     /locus_tag="LP859_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL3028001.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13203
                     /locus_tag="LP859_000011"
                     /pseudo
     CDS             12429..13203
                     /locus_tag="LP859_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            13350..13715
                     /locus_tag="LP859_000012"
     CDS             13350..13715
                     /locus_tag="LP859_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL3028002.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13863..15071)
                     /locus_tag="LP859_000013"
     CDS             complement(13863..15071)
                     /locus_tag="LP859_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3028003.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15090..15368)
                     /locus_tag="LP859_000014"
     CDS             complement(15090..15368)
                     /locus_tag="LP859_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028004.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15522..16217
                     /locus_tag="LP859_000015"
     CDS             15522..16217
                     /locus_tag="LP859_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3028005.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16659..17456)
                     /locus_tag="LP859_000016"
     CDS             complement(16659..17456)
                     /locus_tag="LP859_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL3028006.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17453..18637)
                     /locus_tag="LP859_000017"
     CDS             complement(17453..18637)
                     /locus_tag="LP859_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL3028007.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18662..19909)
                     /locus_tag="LP859_000018"
     CDS             complement(18662..19909)
                     /locus_tag="LP859_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL3028008.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20124..21869
                     /gene="dnaX"
                     /locus_tag="LP859_000019"
     CDS             20124..21869
                     /gene="dnaX"
                     /locus_tag="LP859_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL3028009.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21889..22203
                     /locus_tag="LP859_000020"
     CDS             21889..22203
                     /locus_tag="LP859_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL3028010.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22228..22824
                     /gene="recR"
                     /locus_tag="LP859_000021"
     CDS             22228..22824
                     /gene="recR"
                     /locus_tag="LP859_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL3028011.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22888..23532
                     /locus_tag="LP859_000022"
     CDS             22888..23532
                     /locus_tag="LP859_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL3028012.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23547..23876
                     /locus_tag="LP859_000023"
     CDS             23547..23876
                     /locus_tag="LP859_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL3028013.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23890..24828
                     /gene="holB"
                     /locus_tag="LP859_000024"
     CDS             23890..24828
                     /gene="holB"
                     /locus_tag="LP859_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL3028014.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24864..25688
                     /locus_tag="LP859_000025"
     CDS             24864..25688
                     /locus_tag="LP859_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL3028015.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25681..26028
                     /locus_tag="LP859_000026"
     CDS             25681..26028
                     /locus_tag="LP859_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL3028016.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26097..26969
                     /gene="rsmI"
                     /locus_tag="LP859_000027"
     CDS             26097..26969
                     /gene="rsmI"
                     /locus_tag="LP859_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL3028017.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27078..28199
                     /gene="dinB"
                     /locus_tag="LP859_000028"
     CDS             27078..28199
                     /gene="dinB"
                     /locus_tag="LP859_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL3028018.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28253..28855)
                     /gene="nrdG"
                     /locus_tag="LP859_000029"
     CDS             complement(28253..28855)
                     /gene="nrdG"
                     /locus_tag="LP859_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL3028019.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29170..31359)
                     /gene="nrdD"
                     /locus_tag="LP859_000030"
     CDS             complement(29170..31359)
                     /gene="nrdD"
                     /locus_tag="LP859_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL3028020.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31658..32452
                     /locus_tag="LP859_000031"
     CDS             31658..32452
                     /locus_tag="LP859_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028021.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32442..34484
                     /locus_tag="LP859_000032"
     CDS             32442..34484
                     /locus_tag="LP859_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028022.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34765..34914
                     /locus_tag="LP859_000033"
     CDS             34765..34914
                     /locus_tag="LP859_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL3028023.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34925..36439
                     /gene="dltA"
                     /locus_tag="LP859_000034"
     CDS             34925..36439
                     /gene="dltA"
                     /locus_tag="LP859_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL3028024.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36436..37641
                     /gene="dltB"
                     /locus_tag="LP859_000035"
     CDS             36436..37641
                     /gene="dltB"
                     /locus_tag="LP859_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL3028025.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37686..37919
                     /gene="dltC"
                     /locus_tag="LP859_000036"
     CDS             37686..37919
                     /gene="dltC"
                     /locus_tag="LP859_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL3028026.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37922..39190
                     /gene="dltD"
                     /locus_tag="LP859_000037"
     CDS             37922..39190
                     /gene="dltD"
                     /locus_tag="LP859_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL3028027.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39331..40413
                     /locus_tag="LP859_000038"
     CDS             39331..40413
                     /locus_tag="LP859_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL3028028.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40413..41216
                     /locus_tag="LP859_000039"
     CDS             40413..41216
                     /locus_tag="LP859_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL3028029.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFVFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41286..42026
                     /locus_tag="LP859_000040"
     CDS             41286..42026
                     /locus_tag="LP859_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3028030.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42037..43041
                     /locus_tag="LP859_000041"
     CDS             42037..43041
                     /locus_tag="LP859_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL3028031.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43086..44930)
                     /locus_tag="LP859_000042"
     CDS             complement(43086..44930)
                     /locus_tag="LP859_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028032.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45142..45660
                     /locus_tag="LP859_000043"
     CDS             45142..45660
                     /locus_tag="LP859_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL3028033.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45788..46471
                     /locus_tag="LP859_000044"
     CDS             45788..46471
                     /locus_tag="LP859_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL3028034.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46780..46853
                     /locus_tag="LP859_000045"
     tRNA            46780..46853
                     /locus_tag="LP859_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46814..46816,aa:Arg,seq:tct)
     gene            47012..47371
                     /locus_tag="LP859_000046"
     CDS             47012..47371
                     /locus_tag="LP859_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL3028035.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47494..49125
                     /locus_tag="LP859_000047"
     CDS             47494..49125
                     /locus_tag="LP859_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL3028036.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49187..49828)
                     /locus_tag="LP859_000048"
     CDS             complement(49187..49828)
                     /locus_tag="LP859_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL3028037.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50014..50090
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50110..50418
                     /gene="rplU"
                     /locus_tag="LP859_000049"
     CDS             50110..50418
                     /gene="rplU"
                     /locus_tag="LP859_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL3028038.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50445..50789
                     /locus_tag="LP859_000050"
     CDS             50445..50789
                     /locus_tag="LP859_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL3028039.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50802..51095
                     /gene="rpmA"
                     /locus_tag="LP859_000051"
     CDS             50802..51095
                     /gene="rpmA"
                     /locus_tag="LP859_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL3028040.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51529..54336
                     /locus_tag="LP859_000052"
     CDS             51529..54336
                     /locus_tag="LP859_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3028041.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54527..55018
                     /locus_tag="LP859_000053"
     CDS             54527..55018
                     /locus_tag="LP859_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3028042.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55121..56110
                     /gene="manX"
                     /locus_tag="LP859_000054"
     CDS             55121..56110
                     /gene="manX"
                     /locus_tag="LP859_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL3028043.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56143..56946
                     /locus_tag="LP859_000055"
     CDS             56143..56946
                     /locus_tag="LP859_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL3028044.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56967..57881
                     /locus_tag="LP859_000056"
     CDS             56967..57881
                     /locus_tag="LP859_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL3028045.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57964..58338
                     /locus_tag="LP859_000057"
     CDS             57964..58338
                     /locus_tag="LP859_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL3028046.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58477..58722
                     /locus_tag="LP859_000058"
     CDS             58477..58722
                     /locus_tag="LP859_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3028047.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58894..59958
                     /locus_tag="LP859_000059"
     CDS             58894..59958
                     /locus_tag="LP859_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL3028048.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60204..60629
                     /locus_tag="LP859_000060"
     CDS             60204..60629
                     /locus_tag="LP859_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3028049.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60622..61071
                     /gene="nusB"
                     /locus_tag="LP859_000061"
     CDS             60622..61071
                     /gene="nusB"
                     /locus_tag="LP859_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL3028050.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61168..62022
                     /locus_tag="LP859_000062"
     CDS             61168..62022
                     /locus_tag="LP859_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL3028051.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62023..63360
                     /locus_tag="LP859_000063"
     CDS             62023..63360
                     /locus_tag="LP859_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL3028052.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63365..63589
                     /locus_tag="LP859_000064"
     CDS             63365..63589
                     /locus_tag="LP859_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL3028053.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63591..64481
                     /locus_tag="LP859_000065"
     CDS             63591..64481
                     /locus_tag="LP859_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL3028054.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64490..65308
                     /locus_tag="LP859_000066"
     CDS             64490..65308
                     /locus_tag="LP859_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL3028055.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65415..65864
                     /locus_tag="LP859_000067"
     CDS             65415..65864
                     /locus_tag="LP859_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL3028056.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65882..67564
                     /gene="recN"
                     /locus_tag="LP859_000068"
     CDS             65882..67564
                     /gene="recN"
                     /locus_tag="LP859_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL3028057.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67619..67735)
                     /locus_tag="LP859_000069"
     CDS             complement(67619..67735)
                     /locus_tag="LP859_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL3028058.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67808..68764)
                     /locus_tag="LP859_000070"
     CDS             complement(67808..68764)
                     /locus_tag="LP859_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3028059.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68935..69303
                     /locus_tag="LP859_000071"
     CDS             68935..69303
                     /locus_tag="LP859_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL3028060.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69473..69904
                     /gene="mraZ"
                     /locus_tag="LP859_000072"
     CDS             69473..69904
                     /gene="mraZ"
                     /locus_tag="LP859_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL3028061.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69925..70884
                     /gene="rsmH"
                     /locus_tag="LP859_000073"
     CDS             69925..70884
                     /gene="rsmH"
                     /locus_tag="LP859_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL3028062.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70889..71290
                     /gene="ftsL"
                     /locus_tag="LP859_000074"
     CDS             70889..71290
                     /gene="ftsL"
                     /locus_tag="LP859_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL3028063.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71291..73483
                     /locus_tag="LP859_000075"
     CDS             71291..73483
                     /locus_tag="LP859_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL3028064.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73509..74471
                     /locus_tag="LP859_000076"
     CDS             73509..74471
                     /locus_tag="LP859_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL3028065.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74485..75846
                     /locus_tag="LP859_000077"
     CDS             74485..75846
                     /locus_tag="LP859_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL3028066.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75872..76960
                     /gene="murG"
                     /locus_tag="LP859_000078"
     CDS             75872..76960
                     /gene="murG"
                     /locus_tag="LP859_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL3028067.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76975..78195
                     /locus_tag="LP859_000079"
     CDS             76975..78195
                     /locus_tag="LP859_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL3028068.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78351..79676
                     /gene="ftsA"
                     /locus_tag="LP859_000080"
     CDS             78351..79676
                     /gene="ftsA"
                     /locus_tag="LP859_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL3028069.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79699..80940
                     /gene="ftsZ"
                     /locus_tag="LP859_000081"
     CDS             79699..80940
                     /gene="ftsZ"
                     /locus_tag="LP859_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL3028070.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80957..81634
                     /locus_tag="LP859_000082"
     CDS             80957..81634
                     /locus_tag="LP859_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL3028071.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81648..82247
                     /locus_tag="LP859_000083"
     CDS             81648..82247
                     /locus_tag="LP859_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL3028072.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82262..82549
                     /locus_tag="LP859_000084"
     CDS             82262..82549
                     /locus_tag="LP859_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL3028073.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82598..83380
                     /locus_tag="LP859_000085"
     CDS             82598..83380
                     /locus_tag="LP859_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL3028074.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83401..84108
                     /locus_tag="LP859_000086"
     CDS             83401..84108
                     /locus_tag="LP859_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL3028075.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84370..87168
                     /gene="ileS"
                     /locus_tag="LP859_000087"
     CDS             84370..87168
                     /gene="ileS"
                     /locus_tag="LP859_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL3028076.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87217..88743)
                     /locus_tag="LP859_000088"
     CDS             complement(87217..88743)
                     /locus_tag="LP859_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL3028077.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88758..89405)
                     /locus_tag="LP859_000089"
     CDS             complement(88758..89405)
                     /locus_tag="LP859_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL3028078.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89589..89918)
                     /locus_tag="LP859_000090"
     CDS             complement(89589..89918)
                     /locus_tag="LP859_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL3028079.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90095..90823
                     /gene="sfsA"
                     /locus_tag="LP859_000091"
     CDS             90095..90823
                     /gene="sfsA"
                     /locus_tag="LP859_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL3028080.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90839..91852
                     /locus_tag="LP859_000092"
     CDS             90839..91852
                     /locus_tag="LP859_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3028081.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91852..93129
                     /locus_tag="LP859_000093"
     CDS             91852..93129
                     /locus_tag="LP859_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL3028082.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93192..95894
                     /locus_tag="LP859_000094"
     CDS             93192..95894
                     /locus_tag="LP859_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3028083.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96046..96363
                     /locus_tag="LP859_000095"
     CDS             96046..96363
                     /locus_tag="LP859_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3028084.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96393..96713
                     /locus_tag="LP859_000096"
     CDS             96393..96713
                     /locus_tag="LP859_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL3028085.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96821..98281
                     /locus_tag="LP859_000097"
     CDS             96821..98281
                     /locus_tag="LP859_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3028086.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98349..98570
                     /locus_tag="LP859_000098"
     CDS             98349..98570
                     /locus_tag="LP859_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028087.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98601..98783
                     /locus_tag="LP859_000099"
     CDS             98601..98783
                     /locus_tag="LP859_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL3028088.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98783..99196
                     /locus_tag="LP859_000100"
     CDS             98783..99196
                     /locus_tag="LP859_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL3028089.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99319..100500
                     /locus_tag="LP859_000101"
     CDS             99319..100500
                     /locus_tag="LP859_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3028090.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100571..100735)
                     /locus_tag="LP859_000102"
     CDS             complement(100571..100735)
                     /locus_tag="LP859_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028091.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100889..100962
                     /locus_tag="LP859_000103"
     tRNA            100889..100962
                     /locus_tag="LP859_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100923..100925,aa:Arg,seq:cct)
     gene            complement(101031..102170)
                     /locus_tag="LP859_000104"
     CDS             complement(101031..102170)
                     /locus_tag="LP859_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3028092.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102469..103104)
                     /locus_tag="LP859_000105"
     CDS             complement(102469..103104)
                     /locus_tag="LP859_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028093.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103217..103852)
                     /locus_tag="LP859_000106"
     CDS             complement(103217..103852)
                     /locus_tag="LP859_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL3028094.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103886..104347)
                     /locus_tag="LP859_000107"
     CDS             complement(103886..104347)
                     /locus_tag="LP859_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL3028095.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104477..104908)
                     /locus_tag="LP859_000108"
     CDS             complement(104477..104908)
                     /locus_tag="LP859_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL3028096.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104926..105246)
                     /locus_tag="LP859_000109"
     CDS             complement(104926..105246)
                     /locus_tag="LP859_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028097.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105545..106321
                     /locus_tag="LP859_000110"
     CDS             105545..106321
                     /locus_tag="LP859_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL3028098.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106336..106539
                     /locus_tag="LP859_000111"
     CDS             106336..106539
                     /locus_tag="LP859_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028099.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106555..106893
                     /locus_tag="LP859_000112"
     CDS             106555..106893
                     /locus_tag="LP859_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL3028100.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106880..107059
                     /locus_tag="LP859_000113"
     CDS             106880..107059
                     /locus_tag="LP859_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028101.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107102..107572)
                     /locus_tag="LP859_000114"
     CDS             complement(107102..107572)
                     /locus_tag="LP859_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028102.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107659..108357
                     /locus_tag="LP859_000115"
     CDS             107659..108357
                     /locus_tag="LP859_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL3028103.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108535..108876
                     /locus_tag="LP859_000116"
     CDS             108535..108876
                     /locus_tag="LP859_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028104.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108869..109540
                     /locus_tag="LP859_000117"
     CDS             108869..109540
                     /locus_tag="LP859_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL3028105.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109546..110232
                     /locus_tag="LP859_000118"
     CDS             109546..110232
                     /locus_tag="LP859_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028106.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            110235..110984
                     /locus_tag="LP859_000119"
     CDS             110235..110984
                     /locus_tag="LP859_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028107.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110996..111265
                     /locus_tag="LP859_000120"
     CDS             110996..111265
                     /locus_tag="LP859_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028108.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            111271..111435
                     /locus_tag="LP859_000121"
     CDS             111271..111435
                     /locus_tag="LP859_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028109.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111428..111727
                     /locus_tag="LP859_000122"
     CDS             111428..111727
                     /locus_tag="LP859_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028110.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            111727..112029
                     /locus_tag="LP859_000123"
     CDS             111727..112029
                     /locus_tag="LP859_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL3028111.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            112069..112434
                     /locus_tag="LP859_000124"
     CDS             112069..112434
                     /locus_tag="LP859_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028112.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            112924..113064
                     /locus_tag="LP859_000125"
     CDS             112924..113064
                     /locus_tag="LP859_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028113.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            113061..113249
                     /locus_tag="LP859_000126"
     CDS             113061..113249
                     /locus_tag="LP859_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028114.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            113257..113451
                     /locus_tag="LP859_000127"
     CDS             113257..113451
                     /locus_tag="LP859_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028115.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(113484..113696)
                     /locus_tag="LP859_000128"
     CDS             complement(113484..113696)
                     /locus_tag="LP859_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028116.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            113821..114024
                     /locus_tag="LP859_000129"
     CDS             113821..114024
                     /locus_tag="LP859_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028117.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            114021..114293
                     /locus_tag="LP859_000130"
     CDS             114021..114293
                     /locus_tag="LP859_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL3028118.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            114294..114479
                     /locus_tag="LP859_000131"
     CDS             114294..114479
                     /locus_tag="LP859_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028119.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            114466..114813
                     /locus_tag="LP859_000132"
     CDS             114466..114813
                     /locus_tag="LP859_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028120.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            114773..115249
                     /locus_tag="LP859_000133"
     CDS             114773..115249
                     /locus_tag="LP859_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL3028121.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(115395..116123)
                     /locus_tag="LP859_000134"
     CDS             complement(115395..116123)
                     /locus_tag="LP859_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL3028122.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            116171..116449
                     /locus_tag="LP859_000135"
     CDS             116171..116449
                     /locus_tag="LP859_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028123.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            116451..116837
                     /locus_tag="LP859_000136"
     CDS             116451..116837
                     /locus_tag="LP859_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028124.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            116834..117214
                     /locus_tag="LP859_000137"
     CDS             116834..117214
                     /locus_tag="LP859_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL3028125.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            117326..117778
                     /locus_tag="LP859_000138"
     CDS             117326..117778
                     /locus_tag="LP859_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL3028126.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            117775..119502
                     /locus_tag="LP859_000139"
     CDS             117775..119502
                     /locus_tag="LP859_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL3028127.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            119566..120753
                     /locus_tag="LP859_000140"
     CDS             119566..120753
                     /locus_tag="LP859_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL3028128.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            120725..121294
                     /locus_tag="LP859_000141"
     CDS             120725..121294
                     /locus_tag="LP859_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL3028129.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            121307..122671
                     /locus_tag="LP859_000142"
     CDS             121307..122671
                     /locus_tag="LP859_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL3028130.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            122673..122954
                     /locus_tag="LP859_000143"
     CDS             122673..122954
                     /locus_tag="LP859_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL3028131.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            122932..123258
                     /locus_tag="LP859_000144"
     CDS             122932..123258
                     /locus_tag="LP859_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL3028132.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            123248..123586
                     /locus_tag="LP859_000145"
     CDS             123248..123586
                     /locus_tag="LP859_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028133.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            123576..123941
                     /locus_tag="LP859_000146"
     CDS             123576..123941
                     /locus_tag="LP859_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL3028134.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            123948..124574
                     /locus_tag="LP859_000147"
     CDS             123948..124574
                     /locus_tag="LP859_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3028135.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            124574..125038
                     /locus_tag="LP859_000148"
     CDS             124574..125038
                     /locus_tag="LP859_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028136.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            125233..127542
                     /locus_tag="LP859_000149"
     CDS             125233..127542
                     /locus_tag="LP859_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL3028137.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            127554..128258
                     /locus_tag="LP859_000150"
     CDS             127554..128258
                     /locus_tag="LP859_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3028138.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            128255..131014
                     /locus_tag="LP859_000151"
     CDS             128255..131014
                     /locus_tag="LP859_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3028139.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            131027..>131256
                     /locus_tag="LP859_000152"
     CDS             131027..>131256
                     /locus_tag="LP859_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBL3028140.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT        43544 a        22016 c        27507 g        38189 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca aaaaattgca
    13081 cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt gacaagtaaa
    13141 gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg tctgtacgga
    13201 taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac cttatctttg
    13261 ctcgcttttc tcagccgatg gtataataat gaaaaggtta tcttgttaga taatcttact
    13321 tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg gtagtgaagg
    13381 caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt gttagcttta
    13441 tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta ggggatctgt
    13501 tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt tcagctgcag
    13561 ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg atgaaagtat
    13621 tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg ttgctaagtc
    13681 aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact attcttatcg
    13741 ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata aactattttt
    13801 actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc tattcatcaa
    13861 gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg actatgtttt
    13921 cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc aatcacatac
    13981 aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc aacgcctaag
    14041 cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg ttcgcggacg
    14101 atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag gccgactaat
    14161 gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa taatcctgct
    14221 gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc gaaaatcctt
    14281 ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa aaatgaccca
    14341 gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac acttgaataa
    14401 gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc aataaaacta
    14461 ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat attttttaca
    14521 ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa ccagataatg
    14581 aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac agcggcagct
    14641 aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc agggatatat
    14701 gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc attcaagaat
    14761 cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt tgaaaggaga
    14821 tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa ctgtttcccc
    14881 atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa acctgcttgg
    14941 ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat caacagataa
    15001 taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt attaaataat
    15061 ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa tttttgtatt
    15121 tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg aataaataaa
    15181 tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg attctcatcc
    15241 actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac taaagaagct
    15301 ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt gttcatccag
    15361 taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat aacgaataaa
    15421 aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt taatatcatt
    15481 agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt ttagatgaaa
    15541 gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg gataaaaatg
    15601 attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg atggaagatt
    15661 cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca aatgatggga
    15721 gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta aaagacgaat
    15781 attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat gcagagtttt
    15841 atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag ctacaacact
    15901 atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc
    15961 tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat gatttattcg
    16021 gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa gaaatcgcca
    16081 aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag
    16141 atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac ttgttagaag
    16201 acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac ctgagtatta
    16261 gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    16321 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tctgagtcac
    16381 aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag
    16441 gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct gcatcaagta
    16501 ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga cttatcaccg
    16561 tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac acgatttttg
    16621 tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct gccgccaccg
    16681 ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct tccaggaggt
    16741 gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc ggttttttca
    16801 gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga tttcaattga
    16861 taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa aacaacagca
    16921 atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct
    16981 acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt agacaggtaa
    17041 gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc atctaagatc
    17101 gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga aatgtagatt
    17161 tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc gactgcgtct
    17221 cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt cgttacgatc
    17281 aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa agattggatt
    17341 tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc atgagtagcc
    17401 accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt catcatatca
    17461 agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc
    17521 ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt ccattcattg
    17581 cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc cataccggca
    17641 gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg
    17701 ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt aattcagatt
    17761 gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg aacccggcta
    17821 aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg ccttcgacca
    17881 tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa gaaagcttgc
    17941 ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct atccgccata
    18001 tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct gtagcccaat
    18061 aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa gctttgggaa
    18121 caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct ttctcgacag
    18181 caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg
    18241 gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg atctctgcac
    18301 cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat tcgtccgctg
    18361 tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa
    18421 gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct ttgctttctt
    18481 cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct tcttctgtat
    18541 agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa gtcaacggac
    18601 cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct ttctaaataa
    18661 attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat ttagggatgc
    18721 cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc ttaggctgaa
    18781 cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa ttttctgtcc
    18841 cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga ttttgttttt
    18901 ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga taagaaccat
    18961 ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct tcattttttc
    19021 cggctgcttc cataccacgt gcaatggaac cttgcacgta tccttcacgt acgctaggat
    19081 cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca tcggtgtaag
    19141 aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc caagaatcat
    19201 ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca taagaaatcc
    19261 gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg aattcagcta
    19321 aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg attgcgtttg
    19381 tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc cgtaagaata
    19441 attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc
    19501 cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa
    19561 atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg aacatggaag
    19621 gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg atgatttttc
    19681 caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt ccgtctgtta
    19741 aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg actttgacac
    19801 cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct agccattgat
    19861 ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata aaaaattcct
    19921 cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata ggaagatgtg
    19981 aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt aaatacctga
    20041 aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag agatagtgaa
    20101 tttttaaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc tggcgttcgc
    20161 agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa aatgctattg
    20221 tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc ggaaagacga
    20281 gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac ggtgagccat
    20341 gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa
    20401 tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt
    20461 atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat atgctttcaa
    20521 caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt gtcatattca
    20581 tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg actcagcgat
    20641 ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt atcttgaaag
    20701 aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg
    20761 ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa ggaacaatta
    20821 ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg gacagttact
    20881 tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac caaattctcg
    20941 cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc cgtgacttgc
    21001 tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc agaagtacat
    21061 ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata aagattttga
    21121 gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg gaagtcgcta
    21181 cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta gcatttgcag
    21241 atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac gaaatcaagg
    21301 aactacgcag taatacagga attgagaata caaaagtaca gccggtacag caaacaaatc
    21361 ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa gttctgcggg
    21421 aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc
    21481 tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct ggacctacag
    21541 gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat gacgaagaat
    21601 tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg cctgatcctg
    21661 tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg caggcacaag
    21721 aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc gagttgcttc
    21781 ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct
    21841 ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat gatgcgtgga
    21901 atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa
    21961 gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga attggtcaaa
    22021 gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat
    22081 cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca
    22141 caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt gccaggattt
    22201 tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc gaaactgatc
    22261 gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact cgccttttat
    22321 acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa
    22381 cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc ctgcgaaata
    22441 tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta
    22501 atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca tggtgtgtta
    22561 tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt gcagcggctg
    22621 catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga gggagaagca
    22681 acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca
    22741 catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt attgaaagct
    22801 gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat acaatataaa
    22861 agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat cgaggggcct
    22921 gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa agaagtagca
    22981 aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga agagatccga
    23041 gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc actgctttat
    23101 gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa
    23161 atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc
    23221 atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac tacgccggat
    23281 tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa gaaaaacaga
    23341 caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg tgtacgtcat
    23401 gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga tgcaagaaaa
    23461 agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta tccgcaattt
    23521 tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc caagacaaag
    23581 acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca aaactatcat
    23641 cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt gaagatgacc
    23701 gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag caatttgtat
    23761 ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca gtagaagtag
    23821 aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa ttttaggagg
    23881 agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt gtatcagcag
    23941 cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga aggggagaaa
    24001 ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg tacccaaatg
    24061 aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat
    24121 ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca gatcagacgt
    24181 ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa
    24241 gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt ggaagagccc
    24301 cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct ccctactatt
    24361 caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact gatccatcga
    24421 ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt aacaaacagt
    24481 ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa ggacttgatt
    24541 tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa
    24601 aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc tgtcttgatg
    24661 ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa
    24721 cagataaacc agataattga acggatattg atcgcagagc agaaactacg cagcaatgtc
    24781 agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct tgccgcaaag
    24841 gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat agagatgcag
    24901 gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac aaggtattag
    24961 tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa gagattgatc
    25021 ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa gctgaattag
    25081 aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag
    25141 tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt gatcgaaata
    25201 aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg gtaaaagatc
    25261 tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa
    25321 agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct tttttaggcg
    25381 atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg aatccaacaa
    25441 aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc gaagaatacg
    25501 agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct gatggtaaag
    25561 ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg actggacggg
    25621 aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta ggtgagatga
    25681 atggataaga gatcattata cgacggattg aatcaattag aatcagagtt gagaaataca
    25741 ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact
    25801 ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca agttgcacaa
    25861 gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt
    25921 tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga caatgacgag
    25981 ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc aataacaaga
    26041 ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg tgacagatgt
    26101 ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt gtaccgacgc
    26161 caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa gaatccgatc
    26221 ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt gaaatccata
    26281 caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa ttgattgctt
    26341 tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct tccatcagcg
    26401 atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg atctctatcc
    26461 caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct caaccatttt
    26521 tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa
    26581 aagaacagac agttacgctg attttttatg agtcacctta tcggatcact gctacattga
    26641 cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg
    26701 tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc gaagaacatc
    26761 cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg
    26821 aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc gcttcaggcg
    26881 aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag
    26941 tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa tgaaagacta
    27001 atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata
    27061 atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat acatcaagaa
    27121 aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga gatcatcctg
    27181 agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt gggcgtggag
    27241 tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca atgagtgcac
    27301 aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat cataaataca
    27361 gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt gtggaaccag
    27421 ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc aagtcagcca
    27481 tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta acatgttctg
    27541 cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa aaacctagag
    27601 gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg ccaattgaaa
    27661 aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg atttatacag
    27721 gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga aaaatgggct
    27781 attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt acaagagagc
    27841 gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa gaagcagtgc
    27901 tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga gtccaaaaac
    27961 atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg accaaaagag
    28021 tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca agccttatat
    28081 gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact ttgacgaatc
    28141 tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa
    28201 aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt ttttatatca
    28261 agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt tcccaaatca
    28321 caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg ctacttccgc
    28381 gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca aggatatcga
    28441 tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca aaggtatagc
    28501 ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt cgtacgactt
    28561 gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt
    28621 aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat ggtttgccat
    28681 atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag cagccactaa
    28741 cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa
    28801 tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt ctcataaaaa
    28861 aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta ctcgaccaca
    28921 taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt attttgatag
    28981 agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc
    29041 tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc ccaactaaac
    29101 gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca ggacagcttt
    29161 ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc ggccatgtac
    29221 catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta catcacatgt
    29281 ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg gtttgaaatc
    29341 accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa gatacccgat
    29401 tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt ggagcatagg
    29461 gtattcacag tagtggatga atccacctga acaatatttt ggataatcct tctcaaattc
    29521 caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt ttgtataata
    29581 ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac ggcagaaacg
    29641 gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat attcatcccc
    29701 ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag cttttgcttc
    29761 tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca atccaatgta
    29821 gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat cggtacgtga
    29881 aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg gaatggcttc
    29941 tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc ggtcagacag
    30001 taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg gcaaattcaa
    30061 tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt cgtctttcca
    30121 cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta attcaacgat
    30181 tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca atgctagttg
    30241 tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca aagtgaagat
    30301 cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc gaatctgtaa
    30361 aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc ctaatgaagt
    30421 gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca aactttgcat
    30481 agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc gttctggcga
    30541 atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg caaaaggagc
    30601 aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg ctacattggc
    30661 aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt cgacttcggc
    30721 attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt
    30781 catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt tttgatgcgc
    30841 atttgctata tgtggtggca acattttcag tccgattgat tttccaacga tcccggctgt
    30901 cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt tcacgacagt
    30961 ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt tactgcgagc
    31021 aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt cgtttttttc
    31081 tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt cagcaaaccg
    31141 gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat aagtaagtgg
    31201 tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga tcttttgttc
    31261 atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg tctctggctg
    31321 ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact cctgctaaat
    31381 tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg gctatatata
    31441 gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat tttattgtga
    31501 aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata
    31561 aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa
    31621 attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga
    31681 agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc
    31741 tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat
    31801 tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt
    31861 gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga
    31921 gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga
    31981 taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga
    32041 ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa
    32101 acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact
    32161 tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc
    32221 ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact
    32281 gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga
    32341 tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt
    32401 aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg
    32461 ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat
    32521 ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag
    32581 caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg
    32641 ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa
    32701 taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag
    32761 aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt attatttttt
    32821 ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt
    32881 ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag
    32941 tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa
    33001 aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca
    33061 tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt
    33121 catggatgat cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt
    33181 ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga
    33241 tcatattgag taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca
    33301 cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa
    33361 tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga
    33421 aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga
    33481 ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg
    33541 ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg
    33601 aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat
    33661 cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat
    33721 atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa
    33781 gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg
    33841 tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa
    33901 ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg
    33961 tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac
    34021 ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct
    34081 cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca
    34141 cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca gtccggcaat
    34201 tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac
    34261 aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga
    34321 tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg
    34381 ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt
    34441 atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca
    34501 agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg ttgtgacacg
    34561 atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa
    34621 aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa
    34681 aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt
    34741 gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat
    34801 tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat
    34861 ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa
    34921 gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga
    34981 tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga
    35041 cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg
    35101 caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta
    35161 tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa
    35221 accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat
    35281 aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag
    35341 cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa
    35401 aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt
    35461 tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt
    35521 attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt
    35581 tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt
    35641 ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt
    35701 gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag
    35761 tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac
    35821 tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc
    35881 gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc
    35941 aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa
    36001 caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac
    36061 aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca
    36121 gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt atctgctcca
    36181 agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca
    36241 gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa
    36301 atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca
    36361 ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc
    36421 tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt
    36481 tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga
    36541 ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat
    36601 tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg
    36661 ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt
    36721 ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca
    36781 tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg
    36841 gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg
    36901 ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag
    36961 ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt
    37021 tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct
    37081 gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg
    37141 tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt
    37201 acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg
    37261 aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat
    37321 gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta
    37381 atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg
    37441 tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg
    37501 atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta
    37561 gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta
    37621 gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa
    37681 ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa
    37741 gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc
    37801 aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc
    37861 gagaggattg gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag
    37921 tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt
    37981 tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc
    38041 ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc
    38101 tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca
    38161 tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc
    38221 cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa
    38281 caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga
    38341 ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa
    38401 agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga
    38461 gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga
    38521 atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag
    38581 caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa
    38641 acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac
    38701 gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc
    38761 agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt
    38821 tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc
    38881 ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc
    38941 caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa
    39001 tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt
    39061 ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta
    39121 tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca
    39181 attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct
    39241 gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca
    39301 caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt
    39361 gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga
    39421 gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc
    39481 aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt
    39541 tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg
    39601 tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc
    39661 cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct
    39721 tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag
    39781 tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc
    39841 agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga
    39901 gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg
    39961 caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt
    40021 gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca
    40081 gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat
    40141 aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat
    40201 acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt
    40261 ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat
    40321 gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt
    40381 gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat
    40441 atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt
    40501 tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc
    40561 cggaagaaag catgcagcag cacttattga gttttctagt cgataattta cttttattcg
    40621 tattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa
    40681 gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt
    40741 tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa
    40801 tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg
    40861 tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg
    40921 tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt
    40981 tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag
    41041 atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag
    41101 caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag
    41161 aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg
    41221 taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat
    41281 gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga
    41341 caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta
    41401 cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc
    41461 attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc
    41521 ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact
    41581 ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat
    41641 taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg
    41701 ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt
    41761 atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc
    41821 tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg
    41881 cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag
    41941 aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg
    42001 cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac
    42061 cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat
    42121 gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat
    42181 acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc
    42241 ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg
    42301 cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca
    42361 cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat
    42421 ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga
    42481 acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac
    42541 aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt
    42601 cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa
    42661 attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg
    42721 aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag
    42781 tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat
    42841 ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta
    42901 ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta
    42961 tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa
    43021 ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct
    43081 attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa
    43141 gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt
    43201 aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta
    43261 tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac
    43321 aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct
    43381 aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga
    43441 tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag
    43501 ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct
    43561 tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc
    43621 agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt
    43681 cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt
    43741 ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt
    43801 agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc
    43861 gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct
    43921 acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg
    43981 cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa
    44041 gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac
    44101 cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag
    44161 ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa
    44221 atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt
    44281 gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata
    44341 gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa
    44401 ctaatcaatg gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga
    44461 taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc
    44521 acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc
    44581 ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg
    44641 tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga
    44701 atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc
    44761 cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag
    44821 aaataagcaa ttcctgttaa aaataacaat gataatataa tgattatgct ccactttata
    44881 cgtaaataaa aagattcttt tttattatat atgttattaa aaaaagacat aaaggtattc
    44941 ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa
    45001 taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac
    45061 atttttacag ctataatttg aaaaaaattt atcgttatta tatgattaag aaggattgaa
    45121 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
    45181 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
    45241 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
    45301 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
    45361 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
    45421 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
    45481 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
    45541 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
    45601 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
    45661 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
    45721 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
    45781 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
    45841 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
    45901 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
    45961 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
    46021 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
    46081 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
    46141 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
    46201 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
    46261 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
    46321 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
    46381 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
    46441 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
    46501 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
    46561 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
    46621 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
    46681 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
    46741 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
    46801 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
    46861 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
    46921 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
    46981 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
    47041 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
    47101 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
    47161 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
    47221 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
    47281 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
    47341 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
    47401 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
    47461 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
    47521 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
    47581 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
    47641 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
    47701 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
    47761 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
    47821 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
    47881 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
    47941 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
    48001 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
    48061 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
    48121 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
    48181 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
    48241 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
    48301 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
    48361 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
    48421 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
    48481 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
    48541 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
    48601 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
    48661 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
    48721 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
    48781 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
    48841 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
    48901 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
    48961 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
    49021 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
    49081 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
    49141 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
    49201 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
    49261 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
    49321 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
    49381 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
    49441 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
    49501 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
    49561 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
    49621 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
    49681 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
    49741 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
    49801 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
    49861 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
    49921 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
    49981 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
    50041 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
    50101 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
    50161 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
    50221 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
    50281 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
    50341 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
    50401 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
    50461 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
    50521 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
    50581 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
    50641 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
    50701 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
    50761 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
    50821 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
    50881 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
    50941 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
    51001 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
    51061 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
    51121 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
    51181 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
    51241 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
    51301 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
    51361 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
    51421 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
    51481 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
    51541 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
    51601 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
    51661 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
    51721 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
    51781 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
    51841 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
    51901 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
    51961 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
    52021 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
    52081 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
    52141 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
    52201 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
    52261 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
    52321 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
    52381 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
    52441 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
    52501 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
    52561 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
    52621 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
    52681 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
    52741 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
    52801 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
    52861 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
    52921 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
    52981 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
    53041 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
    53101 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
    53161 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
    53221 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
    53281 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
    53341 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
    53401 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
    53461 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
    53521 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
    53581 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
    53641 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
    53701 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
    53761 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
    53821 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
    53881 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
    53941 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
    54001 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
    54061 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
    54121 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
    54181 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
    54241 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
    54301 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
    54361 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
    54421 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
    54481 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
    54541 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
    54601 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
    54661 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
    54721 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
    54781 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
    54841 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
    54901 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
    54961 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
    55021 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
    55081 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    55141 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    55201 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    55261 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    55321 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    55381 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    55441 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    55501 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    55561 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    55621 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    55681 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    55741 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    55801 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    55861 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    55921 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    55981 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    56041 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    56101 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    56161 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    56221 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    56281 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    56341 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    56401 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    56461 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    56521 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    56581 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    56641 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    56701 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    56761 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    56821 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    56881 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    56941 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    57001 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    57061 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    57121 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    57181 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    57241 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    57301 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    57361 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    57421 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    57481 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    57541 tcgtattagc tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa
    57601 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    57661 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    57721 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    57781 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    57841 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    57901 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    57961 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    58021 ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg
    58081 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    58141 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    58201 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    58261 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    58321 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    58381 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    58441 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    58501 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    58561 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    58621 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    58681 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    58741 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    58801 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    58861 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    58921 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    58981 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    59041 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    59101 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    59161 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    59221 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    59281 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    59341 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    59401 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    59461 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    59521 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    59581 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    59641 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    59701 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    59761 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    59821 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    59881 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    59941 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    60001 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    60061 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    60121 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    60181 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    60241 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    60301 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    60361 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    60421 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    60481 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    60541 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    60601 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    60661 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    60721 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    60781 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    60841 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    60901 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    60961 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    61021 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    61081 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    61141 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    61201 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    61261 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    61321 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    61381 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    61441 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    61501 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    61561 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    61621 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    61681 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    61741 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    61801 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    61861 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    61921 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    61981 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    62041 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    62101 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    62161 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    62221 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    62281 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    62341 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    62401 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    62461 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    62521 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    62581 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    62641 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    62701 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    62761 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    62821 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    62881 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    62941 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    63001 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    63061 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    63121 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    63181 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    63241 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    63301 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    63361 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    63421 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    63481 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    63541 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    63601 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg
    63661 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    63721 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    63781 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    63841 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    63901 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    63961 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    64021 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    64081 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc
    64141 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    64201 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    64261 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    64321 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    64381 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    64441 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    64501 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    64561 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    64621 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    64681 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    64741 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    64801 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    64861 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    64921 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    64981 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    65041 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    65101 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    65161 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    65221 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    65281 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    65341 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    65401 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    65461 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    65521 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    65581 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    65641 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    65701 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    65761 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    65821 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    65881 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    65941 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    66001 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    66061 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    66121 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    66181 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    66241 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    66301 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    66361 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    66421 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    66481 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    66541 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    66601 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    66661 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    66721 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    66781 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    66841 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    66901 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    66961 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    67021 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    67081 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    67141 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    67201 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    67261 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    67321 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    67381 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    67441 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    67501 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    67561 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    67621 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    67681 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    67741 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    67801 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    67861 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    67921 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    67981 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    68041 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    68101 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    68161 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    68221 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    68281 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    68341 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    68401 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    68461 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    68521 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    68581 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    68641 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    68701 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    68761 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    68821 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    68881 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    68941 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    69001 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    69061 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    69121 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    69181 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    69241 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    69301 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    69361 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    69421 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    69481 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    69541 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    69601 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    69661 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    69721 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    69781 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    69841 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    69901 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    69961 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    70021 ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat
    70081 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    70141 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    70201 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    70261 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    70321 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    70381 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    70441 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    70501 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    70561 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    70621 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    70681 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    70741 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    70801 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    70861 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    70921 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    70981 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    71041 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    71101 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    71161 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    71221 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    71281 agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa
    71341 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt
    71401 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    71461 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    71521 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    71581 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    71641 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    71701 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    71761 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    71821 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    71881 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    71941 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    72001 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    72061 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    72121 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    72181 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    72241 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    72301 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    72361 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    72421 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    72481 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    72541 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    72601 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    72661 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    72721 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    72781 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    72841 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa
    72901 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    72961 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    73021 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    73081 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    73141 agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    73201 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    73261 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    73321 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    73381 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    73441 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    73501 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    73561 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    73621 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    73681 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    73741 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    73801 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    73861 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    73921 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    73981 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    74041 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    74101 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    74161 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    74221 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    74281 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    74341 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    74401 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    74461 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    74521 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    74581 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    74641 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    74701 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    74761 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    74821 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    74881 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    74941 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    75001 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    75061 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    75121 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    75181 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    75241 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    75301 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    75361 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    75421 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    75481 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    75541 gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    75601 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    75661 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    75721 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    75781 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    75841 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    75901 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    75961 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    76021 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    76081 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    76141 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    76201 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    76261 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    76321 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    76381 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    76441 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    76501 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    76561 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    76621 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    76681 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    76741 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    76801 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    76861 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    76921 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    76981 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    77041 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    77101 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    77161 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    77221 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    77281 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    77341 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    77401 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    77461 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    77521 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    77581 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    77641 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    77701 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    77761 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    77821 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    77881 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    77941 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    78001 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    78061 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    78121 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    78181 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    78241 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    78301 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    78361 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    78421 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    78481 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    78541 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    78601 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    78661 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    78721 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    78781 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    78841 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    78901 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    78961 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    79021 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    79081 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    79141 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    79201 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    79261 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    79321 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    79381 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    79441 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    79501 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    79561 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    79621 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    79681 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    79741 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    79801 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    79861 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    79921 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    79981 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    80041 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    80101 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    80161 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    80221 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    80281 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    80341 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    80401 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    80461 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    80521 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    80581 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    80641 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    80701 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    80761 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    80821 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    80881 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    80941 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    81001 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    81061 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    81121 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    81181 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    81241 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    81301 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    81361 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    81421 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    81481 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    81541 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    81601 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    81661 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    81721 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    81781 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    81841 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    81901 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    81961 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    82021 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    82081 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    82141 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    82201 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    82261 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    82321 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    82381 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    82441 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    82501 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    82561 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    82621 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    82681 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    82741 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    82801 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    82861 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    82921 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    82981 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    83041 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    83101 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    83161 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    83221 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    83281 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    83341 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    83401 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    83461 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    83521 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    83581 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    83641 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    83701 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    83761 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    83821 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    83881 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    83941 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    84001 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    84061 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    84121 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    84181 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    84241 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    84301 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    84361 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    84421 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    84481 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    84541 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    84601 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    84661 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    84721 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    84781 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    84841 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac
    84901 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    84961 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    85021 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    85081 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    85141 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    85201 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    85261 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    85321 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    85381 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    85441 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    85501 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    85561 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    85621 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    85681 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    85741 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    85801 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    85861 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    85921 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    85981 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    86041 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    86101 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    86161 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    86221 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    86281 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    86341 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    86401 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    86461 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    86521 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    86581 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    86641 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    86701 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    86761 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    86821 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    86881 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    86941 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    87001 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    87061 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    87121 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    87181 ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc
    87241 acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa
    87301 agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc
    87361 ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga
    87421 gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct
    87481 attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc
    87541 ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat
    87601 cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa
    87661 tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg
    87721 tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt
    87781 gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc
    87841 cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa
    87901 cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat
    87961 ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat
    88021 atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc
    88081 tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg
    88141 agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc
    88201 taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt
    88261 caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa
    88321 gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg
    88381 aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg
    88441 gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata
    88501 gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt
    88561 ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac
    88621 agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt
    88681 acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga
    88741 catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg
    88801 acggggattt tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca
    88861 acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct
    88921 ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt
    88981 tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa
    89041 tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt
    89101 tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa
    89161 aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca
    89221 gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa
    89281 atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg
    89341 actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt
    89401 gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa
    89461 cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa
    89521 aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg
    89581 ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga
    89641 gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga
    89701 tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa
    89761 tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga
    89821 tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt
    89881 gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc
    89941 ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata
    90001 aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg
    90061 ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc
    90121 tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat
    90181 aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga
    90241 agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt
    90301 aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga
    90361 tggtatttta gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa
    90421 gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca
    90481 aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc
    90541 tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga
    90601 tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat
    90661 ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt
    90721 tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat
    90781 tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat
    90841 gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt
    90901 aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga
    90961 atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa
    91021 tattactcat atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc
    91081 taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc
    91141 aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc
    91201 agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga
    91261 cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt
    91321 agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt
    91381 aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta
    91441 tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga
    91501 gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat
    91561 ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt
    91621 tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat
    91681 ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg
    91741 attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt
    91801 aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa
    91861 tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca
    91921 ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg
    91981 gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg
    92041 gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa
    92101 gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg
    92161 ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt
    92221 ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg
    92281 aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt
    92341 gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca
    92401 gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg
    92461 acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga
    92521 aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg tctgattttc
    92581 tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg
    92641 gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc
    92701 aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt
    92761 acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat
    92821 cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat
    92881 aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt
    92941 tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca
    93001 gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa
    93061 gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg
    93121 cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga
    93181 ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac
    93241 aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc
    93301 gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg
    93361 tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc
    93421 aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg
    93481 caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg
    93541 gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc
    93601 tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac
    93661 tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata
    93721 gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt
    93781 taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc
    93841 ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat
    93901 cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga
    93961 atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag
    94021 aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc
    94081 tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa
    94141 aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac
    94201 tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc
    94261 gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct
    94321 ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt
    94381 ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg
    94441 atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt
    94501 tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag
    94561 accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg
    94621 atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt
    94681 ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag
    94741 ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag
    94801 aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga
    94861 ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt
    94921 ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca
    94981 aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa
    95041 acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga
    95101 caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag
    95161 atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg
    95221 aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag
    95281 gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac
    95341 tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg
    95401 aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat
    95461 ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg
    95521 atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct
    95581 tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt
    95641 tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt
    95701 tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa
    95761 aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat
    95821 atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata
    95881 aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact
    95941 ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct
    96001 tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca
    96061 atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa
    96121 gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat
    96181 aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg
    96241 aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg
    96301 caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa
    96361 taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca
    96421 tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg
    96481 ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac
    96541 ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata
    96601 tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct attgcgttta
    96661 ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac
    96721 ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag
    96781 aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg
    96841 gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc
    96901 tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat
    96961 gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt
    97021 agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt
    97081 ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct
    97141 tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat
    97201 tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa
    97261 tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc
    97321 agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt
    97381 tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac
    97441 gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc
    97501 agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga
    97561 tgggcaactg atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc
    97621 tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg
    97681 tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact
    97741 aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt
    97801 agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc
    97861 atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa
    97921 acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa
    97981 cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt
    98041 gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc
    98101 tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac
    98161 aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat
    98221 ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata
    98281 agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat tttctaggag
    98341 gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag
    98401 aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt
    98461 tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca
    98521 aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt
    98581 aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt
    98641 tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca
    98701 caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag
    98761 aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt
    98821 tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc
    98881 agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg
    98941 ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac
    99001 aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga
    99061 ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct
    99121 tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat
    99181 cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa
    99241 gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt
    99301 gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag
    99361 gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa
    99421 cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg
    99481 ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta
    99541 atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat
    99601 cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg
    99661 gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc
    99721 agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag
    99781 cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa
    99841 aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa
    99901 ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt
    99961 tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct
   100021 ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat
   100081 tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta
   100141 atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat
   100201 ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa
   100261 tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta
   100321 gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta
   100381 gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat
   100441 gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga
   100501 taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg
   100561 aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg
   100621 ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt
   100681 ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa
   100741 aggacctccc tacattattt cattctacct ctagtttatc atctgttaag gactagtgga
   100801 aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca
   100861 aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc
   100921 ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtataaata acccctgtat
   100981 cctttgcggt acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca
   101041 tttttatata aactatcgaa cacagtattg attttttctt tatcctgatc ttcaagctct
   101101 cgaacaatat gaagataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
   101161 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgtgt atgccttagt
   101221 ccatgcaaag taaattgctt ttcaatccct aattctttta attttttgcg caaatatttg
   101281 ctgacggcgt ttgatgaaac taatccattt tttaagttga aaaaaacaaa attatgaggg
   101341 tttttgattt caaaattctc gaataattct ttttgattga ttttaaactt ttttaagaga
   101401 tcaatcgtat gtttatcaat ggaaattttc ctatttgaag tttcgttttt tgtttttccc
   101461 cattcattta atttataatt ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg
   101521 atatcttccc atgttaatcc tagaagttca gcaaatcgca ttccagtggc accagcaact
   101581 aacacgagca taggagaaga gtactgcgca gaaaggtttt cttctacgag tttcattaaa
   101641 gatttaaatt gatcataatc taaatattta tcttcttttg gcttcaatga atcttttcct
   101701 ttgattactg cttttctagt tggatcaaac ggaattaatc cttcttctac ggcatctttc
   101761 aacgacgctc taatatggtt attaaactta ataacggtag attttacatg gtctttagca
   101821 tacttattta aaaagcgttg atatcccact ctatctaaat cagaaatcaa taccgctggc
   101881 atatatttct ttatgttcat taacgtatct tcgtattttc tataagtgat aggagatact
   101941 gttccttctt tataaagttg catccaatcc tcaaagtaat ctgataataa tagatttttg
   102001 cgatcccctt taaggccttt gttcaagtta tattctaatt cgttggcggc atcttttgct
   102061 tctccttttg tcttaaatcc tgattttctt atcttcgtgt attttccgtc atcttttttg
   102121 tatgaaattt cgtattgcca actatttcca cgtttgacta atcttgccat aattgaatca
   102181 gctctctttc tctgatacaa taggaactat aaagaagccc attgtttggg ttttattatt
   102241 ttttgcacat cctttttgct tgccggcgca aggatgtgct atttgtcgtt ttaatggtat
   102301 aattggatgt gtattagatc atccaatcag aggtgtttat tatgattaaa gtgattttta
   102361 aaagaataac tgaacttcca gagtttagat tatttgttga ttattggtat gtctggtttt
   102421 ttattgttgc attgctgata ggtctagatg tcatattaga aagaaaactt aagtaagagt
   102481 tgagttaata aattgaattc ctaaatgaga tattttgtag cccatatttt gtttgccgac
   102541 atcacctaat actatggagc taatcgtttt ttctaacaga ccaaaatcag ataatctatt
   102601 taagctacca tctattagct cttgtgaata gccagacatg ctgatatttc cagtgaaatt
   102661 ttcgtttcca cgactcatta agcatgctaa caattgaatc tcaatcgatg ttaattgatt
   102721 cagtacaaga agataatact cctcgttatc ccatgaagcg ctgttaattc gaagtaaaca
   102781 attcttataa agatttttat acatatctat tttattttgt gcctttgatt tttctacctc
   102841 gtcatttatc ccttcgaata ttccaatgag ttggtccttg ttctctaagt tgaaaacatc
   102901 ttctggaatt tgatcgcgaa tgttttctaa tgattcgttt aaatttttgt agaatttctc
   102961 gatgcgtttg aaacgcttct cgtttttaga cccaaaataa agtgactgca caacgccacc
   103021 aatataagga attgctccaa taccgccttc aactattaat tcaagatatt cttttttggt
   103081 tagtttttca ttgctattat ccatagttac ctcctatttt gtcatttcct ttacaagcat
   103141 tgtgactcct gatggcactc ttttgtatag gttttattat tgtgcacaat ctaactctta
   103201 gtggggcgtg gttcatttat tttttactta ctttactatt aatcatatcc cattgagcat
   103261 aggatttttc tatgttcgat cctcttgaac taatttctcc tgtaaaagtt actttatcgc
   103321 cctttgtaaa ggtatcagca ttgtttaagt ccttcgcaaa aattacatat gaaactttag
   103381 gtgttgcaga aatatcattc caacttttat catcgtactc tttgtctgct ataatggcta
   103441 ttctagtagt cattgattcg ataacagttc cttcaaaagt atatgtttta ccactaatat
   103501 atttagtaaa agctgtattt tgctcagtag ctggagtaat agaataataa ctatctaaga
   103561 aagcacccca gtcatgatta tttgtctcta aaaattcttc aaattgagtt aggcttttat
   103621 tgctgctgtt agatgtagta gtttgagtat cagatgatga gctttcctgt ttgttattgt
   103681 catttgcctc gtttgatgag gaaagcgaga aacctacggt agtaaaaata atcccgataa
   103741 taaggagaat cataggtgtt ttcttatttg tttttttgat aaatcttaca attaacatga
   103801 ttaaacctat aataaataaa acaataccag taaaaccaaa tataccgtcc attaatagat
   103861 cctctttctc ttttgatatt gaatattata aaccaagcaa ttcttttttc ttaaaatcga
   103921 attcttcttg attaataatc ccttcatcta acaactcttt atacttttta atttcatctg
   103981 cacttgaaat ttgtgtttct ttggaattat tatctaatat agattgttgc ttctctatat
   104041 atttttttaa attttccatt tcattccaat atttttttgt aaacataacg gtgttttcat
   104101 ctttcgtggc cgctaatata cctccgcgac tttcgtttcc gcctagcaag gtaaattgta
   104161 tataaccatt actcatacct ggttttttta attgtacggc tgatatattt ttaaagggga
   104221 tagttttttc tcctttaata ccttgattaa ctaaatttat aaatcctttt ctaataattg
   104281 aaatagaatc atcagttact ttaatcaagg tttttcctgg agattttatt aaaatctctt
   104341 tttccatttt ttcttcctca tttctgtgat atgatttttt ttgtagaagc tcatatatga
   104401 ggaaagagcc cgtgttgcag cacgggcttt tttaatgttt tgctgaaatc ggttttttta
   104461 ataattcttg atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc
   104521 attggcagac agcataacaa gtgtatctga gcttattctg aatagaataa tactccataa
   104581 atttttcgag attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata
   104641 aaattagaat agctatttta tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag
   104701 tatcgtacaa aactgaaaat tcaaaatgag aggcgctgaa atgtgcaagt tcatgagata
   104761 aatgaaaggc ttctgcagtt tcaccgtata gattttcatt caaaaaaatg attctgggtt
   104821 ttggatagta gaaaccaggc tctttcatct ccatataaac tactttcaaa ttgtattcgc
   104881 tcaacatttc tttcaatttc aaatacatac aaaccatcac tccaactatt cattttcctc
   104941 taaagcttta gcaattgcaa tcgctttacg cattgtctcc ttagatattt cttttccatc
   105001 aaaagaaaaa acagtatcgt cttctgataa atccacatgt ttaggggttt ctctttcttc
   105061 tctacctaga aggtagtcta cagagacatc gaaatagtca gcaatccgac ttaattcatc
   105121 tgaattagga gtattatttt tccactttgc tagataaccg ttagaatatc caaaattaat
   105181 ttctaactgc cgtatagata tctttttcct cttcgccaag tcttttatta tttcataggt
   105241 attcatcgat aaaacaacct ttctaaatgt ttacaaaaaa agtttagaaa aatacgcaga
   105301 aataatttga ctaattctga gtaatgagct atactatatc ttgtaaacag atttaacaac
   105361 taaaaagaca acaaaaaaca ctattgatat gtaaatgcag accgccaaga aagctttaaa
   105421 atcaatgttt ttatgtctta tttaattatg ctcttagtat agagtattac tcagatactg
   105481 tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga atataaaaga aaggaatgag
   105541 aaatatgaac acaccgcaaa tttttaattt cgaacaaaac gaagttcgga caattttagt
   105601 aaatgatgaa ccatattttg taggcaaaga cgttgcgagt gttttgggtt attcaaacac
   105661 taaagatgct ttgtcgcggc atgtagattt ggaagataag atggggtcgc gaatcacgac
   105721 ctcaggtcaa tcaagagaga tgacaatcat caacgaatct ggtttgtaca gtttaatctt
   105781 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacaagtg aagtgttgcc
   105841 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
   105901 tccagataca atcattcaat tagcaactca actaaaagaa gaaagaactg gaagattaat
   105961 cgcagaacaa aagattgcag agtacgaacc taaaatctcc tatttagata gcatattatc
   106021 ttctacagat tcagtaacaa ttagtcagat tgcagcagat tatgggatgt ctccacaaca
   106081 gatgaataaa ttacttcata aactaggtat tcagaaaaaa gtcggtaacc aatggttatt
   106141 gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat acaactgaga tcccgaaagc
   106201 cgatggtggc actaaaattg taatgaatac caaatggaca cagaaagggc gtctatttat
   106261 ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg gatttagagg aaattggtta
   106321 gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc
   106381 aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga
   106441 attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac
   106501 agaattatag attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa
   106561 tatctagaag caaaaatccc aattccagaa ggctatgtaa ttatctccca agtggattat
   106621 gaggagttaa aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa
   106681 cggattaaca gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga
   106741 aatattatcg atatcgagaa aaacaaaaat ggatttgtct attatccagt tgaaggaaga
   106801 gacacatatc tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa
   106861 atcttaagga ggcaagcgga tgggaaaatt taatagagcg ctagtgttca gcgcaccgct
   106921 aatcatctac gctttaggac tttggggaag caggcaagcg ttgataggaa cgattgttta
   106981 catggtttgg atttttatgg ggcttgatga agctgaagct gagtacagag cgaaaaagcc
   107041 aaccgagggg gctgactaaa aatgaataga aaagagaaac tagaatgtat attactatta
   107101 ctcagtttaa ttctttcact aatttctcta ttgggaagtt tgtatttttg atatcaacaa
   107161 ttttgtggat ttgggttcta tttagagtaa atatcatttt tgtttggtga ttaaggagtt
   107221 tattaaaatt aattttccct acttcaaatg caaggtaaca agatatagca cttttaggag
   107281 gaatatttag cggtacagct tcagagtata ttctattatc atcgactgat cctcctttag
   107341 caattaattt cttaaatgga gtagctgacc atctgcggtt taagttagcg gatgaaagtt
   107401 ctaaattaac taacatagct ggttctgttg aatagttaga aatgattact tttgtataaa
   107461 tcatatctgc agcgaagtaa gaagcattaa gttcaacttc tagttgaggg cgcattcctt
   107521 ttatttttat ccctgttaaa aacgtactca atataaaact tattattgac atccattgaa
   107581 aaattgttaa gtgaaaaaaa ttcaaaataa acaccaccag tttttaacta aattatacca
   107641 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtgact
   107701 acgagcttac aagtggcaga aagcttcgag aaaaaacatc aacatgtttt aagagatatt
   107761 gatgcactaa aaaaagatgt gtccaatttt ggacagatgt ttgtagaagg taatgaacca
   107821 gattcatatg gcagaaatcg acgagttttc ttcattagta gagatggatt tttcttgctg
   107881 gctatggggt ttacaggaaa gaaagctatc tacttcaaac aaaaatacat tgaagcattc
   107941 aacgaaatgg aagatgttat tcgcaagaat actgttcctc aaacaattga agatatgatg
   108001 atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaagatact
   108061 atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatat gaaagttatg
   108121 ggagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca
   108181 aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt
   108241 aagagattcg atgatgctgt ttcatttatt gaaatgtggt tgccagaaac tgcgatccgt
   108301 atggaaattg atcaactgaa cagacaacag agactttttg gtgatgacaa tgaatagagc
   108361 tgaagcgcta agaataggga cggcaattgc taattgctgg tggaaatact ataaaccaat
   108421 catcctaagc caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact
   108481 ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa
   108541 aaagaacttt ccactctaga tcaatatttg attgatcctg attggggcaa gccgaaaatt
   108601 gaggaaacaa gtggtcgaaa aatcagacga aatcttttga cgaatgaaga actagcttgg
   108661 gatcaagatg atttaggcaa ccatgtaact atttgggatc atgtttatct tatccatcta
   108721 tcgaagcatt cgaataaacc tgaatatatt tacgtcatcg aagatggctt gattgatgcg
   108781 ctagaagagt acgacagaga taacttgatt gatatctctt attacggacc aggtaagaaa
   108841 tacattgctg aaatggaggc agaatttgat gagtgaaatc aaagggacaa cgaactttga
   108901 aaaacttttt agtcgtaagt taaataaaat tctcaagaaa aaaggaaatt ttgattattt
   108961 atcttgggct cacgcgtggg agattatgaa aaagaatgat ccacaggcaa cggtaactat
   109021 taatgagtac aaacactaca gggttgtttc tggaactcat caagactttc ttgttgagga
   109081 atataaacct tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa
   109141 aggacacacg gaaaccgaat tatttcctgt tttagattat cgaaaccaac cagttgttaa
   109201 accaaatgca atgcaaatca ataactcatt gaagcgatgc tttgtgaaag cattggctct
   109261 acacggactg ggattatatg tatttcaagg ggaagatatt ccaacaccac ctagaatcga
   109321 tacaaagaaa ttaaacatgc tagagacgat tctagaagct ttcaatgagc agatgggtaa
   109381 agatatgacc aaaaccttaa tcgaatatgt taatgagcag acagataaat tagggctctt
   109441 agctgataac gttgaaacta ttgaacagtt aagctatgag caatgtgcct tgatggagcg
   109501 agcaatagca gctaagagaa aagaattaga taagaagtga tatgagtgtt taaaccatta
   109561 atcgattcat attcagcggt tctgaaaaag ttcaaaggaa aagacataag cgcaaccatc
   109621 aatgaggaag tgaacattga tcgactaaag acgatgtatg acggctacga tggtgatcga
   109681 gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg tacagcaacg aaacttcatc
   109741 tatgcgctga taggcgatat ttttatcgat acaggcatgc caacggactt ctggaaggaa
   109801 ttcttctact ttcgttttga aggtgtcaca gggcgcaaaa taagcctgaa agacgaatcg
   109861 aatacaactg tgagtgatgc caatgtctta gcaaatatca tcttagattt catctttgaa
   109921 catcatattc ctttcaaaga aggctatgag attttacctg cgaatcaaga atattacttc
   109981 tacaaatgca tcacaaaaag agtctgctgc atctgtggca aaacaggagc tgacatcgat
   110041 cactttgaca aagcgctagg aagacgaaag cgcaaagaag ttgatcatgc agagtacaca
   110101 tttgcagcac tctgcagaat ccatcacaca gagaagcaca aaataggtgt gattaatttc
   110161 aaaaataaat atcaaatcaa agggatcaaa ttaaaccaga aaacaatcaa aaagttaaat
   110221 attggagggt aaaaatgaca gaacatcgaa gttattatgc gattatacca gccaacgtaa
   110281 ggtacgacaa aagacttaaa ccaaatacta agttgttata cggagagata acggccttgt
   110341 gtaatgaaag aggcttttgt tgggcaggca atgagtactt tgcagattta tatggtgtga
   110401 ataaagagac catatcgcga tgggtaagtg atttgattaa gtttggatac ttgaatcggg
   110461 aaatcattta caaagagggt accaatcaaa taatcaatag gtacctacga attaatcaat
   110521 accctattga cgaaaaacgc aataccccta ttgacgaaaa agtcaaagat aataatacat
   110581 ctattaataa tacatttaat aatacaaaag aatatataag agagttaccg ccttcgaaaa
   110641 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
   110701 atgagcagat ggagaaactc aaaacagaat tccctaatga ttaccaagag cgaatcgaac
   110761 gactgtcaga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
   110821 gaagttgggc aaggaaagaa aaaagtgaac ctaagaacgc aagcagtgga tacaagcgca
   110881 caggaagacg agagaagctt cctgaatggg caatcgacca agaagcctat ctcaagaaaa
   110941 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg gaaaaatgaa
   111001 gatcgattat ctagaactaa ttaatgaaat agcgaattat aaaaagggcg aggaattaga
   111061 cgtcctgaga gacgtatatg atcaactcga agaagctgga attgaaggaa ttaagaatga
   111121 tcgttcgagt tggagtaaac tcagatacta tttcgcactc tatatcgatg gaacgcaatt
   111181 aagaaattta gcatatacca aattactatt cattgattgt gttaaaggct tgcaaaaaca
   111241 tcttaatgaa cttgagcagg tgtaaacaag atggacctaa agacatttac agcacagatc
   111301 gaattaatgc atcaagaagc tttaagacaa agtgtgtcgt acgaagacaa gtggctcaac
   111361 acatttcatg gcggacgtga gagcgcactt gatcaagtac tcaaattact gaaaggagaa
   111421 tgtcgggatg gataagaaag cggcaatgca gcgaattatc gaattgactt attcagaaga
   111481 ttggcaggaa gataaaaaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggac
   111541 tgaaaagccc aaacggagaa cgccgagaaa gattgcaatc tggcatggtg atcgaattct
   111601 agtaacgggt accgctaaac agttatctga aattactggt ctgagcaaaa atattatttg
   111661 ggatagagct aggagcttat ggattgattc aaaaggaaga cagtttaggt atgtggagga
   111721 gaaataatgg acgaactaat cgcgaaagta gagcagtggg caaaagataa gggattggat
   111781 caagcggatt tcagcaagca aatgttaaaa acggtcgaag aaatcgggga agttgccgct
   111841 tctctagcta gaaaagatga acatggttta agagacggaa ttggcgatgt agtagtaacc
   111901 ttgatcatct tggcaatgca aaatgatatg gatttatacg agtgcttgga ttttgcttat
   111961 gacgaaatca aaggtcggac tggaaagatg gtcaatggtg ttttcgttaa gtccagtgac
   112021 ctgaaataat tccattaatg gaagaaataa ctgaatagga ggatagacat gattgatgca
   112081 aaacaagtgc aaaaacagaa agatggaatg ttgatgtttg aagcatatgt tctaccattt
   112141 cttaatcaat ttgaagtttt ggaatgctct gccagcggag aagaattgga gtatgttgta
   112201 attcgtgaaa ccaaagaaaa cgttcagaag ctaaacgaat ttctttgcac tattaactgt
   112261 tgggatatga tagctccagg atttttatgt ccagctatgg gagaattttt ggaatattgt
   112321 cgtcttgaag atgcaggcac tttggattta gcttatctgg tatacaacta cctaaacatc
   112381 aacacagatc atttgtggtt tggcactgcc gaaagaaaat gggtagtccg ctaaccacca
   112441 atatcaatga aaaaaggcgg tttcctgaaa gtggaattgc cgccattgct gtaaggttct
   112501 ttacgcgact caaggtcaca gaaatataaa tgcaaatctc ttttgaagtg atccaaagct
   112561 ctcggaaaat ggaagttttt tgtttcaaag aataaatgag atttgcgtca agaacctgag
   112621 agttatttcc aaaaaagctt ccaaagaacc gaatgagcta aggacatcat gtgtaaacac
   112681 ataatatagc tctcctttca cggctaatca taacatgaaa aatattcgat gtcagtagga
   112741 aaaaaggtga attatgaaaa gaatattaaa ttatcccggc agtaacagat tctgcaatta
   112801 aactccgaag ctgatgtatc agtatcaact gtagaatatt atgagcgtgg agaaagtcaa
   112861 gcagtcacaa atgtaaaaat cgataacgtc aacggtctta tctgtttaga tgaaggcgat
   112921 agtatgaatg aaattgctgc agaccatgaa ttatcagaaa ctgctttaat tgatcaaatc
   112981 gaattagcta gacaatactt tgttccatat gcagatacct ggtccaagca taagaatgac
   113041 atcatttttc cagaggaaca atgagagcaa ctgatccagt aattatcctt gaggaagcca
   113101 aatttatttg gactcacgaa gagatagagc aagcacgctt gctcttttct caaggagtta
   113161 agccgagcaa agtagctgaa ataatgggtc aaaagattct tgatgtcgga ttgcttttgc
   113221 tccatctagc agaaaaaaat ttgatttgag gtgaaaatga tgattcaact tgcaggcatc
   113281 caaacaggga aaatttattt ttctggagaa agcaaaagcg aagccaacca atggctattg
   113341 aaaacttaca cgaacaataa gaagctaagg aaaaaatatc ctgatttgtt gctaaaggat
   113401 gatcagatta tgccggaacc gatgattttg acccgtaagg agggaagttg aaactaaaaa
   113461 accagccgtg tgggctggag ttgctatttc tttttaataa tgagtttttc taaatttaat
   113521 tcattctcac gagtttgcgt ccaataatca gaataatatt ttaattgttt aagagtagga
   113581 atgtcgtctt cggtaaaatt agaaaataat tctggagaat tatctttttt taattgttct
   113641 aatcctgatt ctttaaaacg ttgttcaacg agttttacta aattgttgat attcatgata
   113701 aatcaccttt ttaaatttaa tttcgatttg attaatcgat acaagtatta tatcaaataa
   113761 aaaagctgat ttagggggtc ttacaaaaac actaaaattt tttcaaaaag aggaggaacg
   113821 gtggtgaaag tcagagaaca attcaagata gtgagcgacg aagataatgt ttacaaaata
   113881 aagatcgtta atattaatga atatcgagaa gcagatcaca aatatttggt tgacttatac
   113941 atgaacgata aatatttggg agaaggattt tgtggagatt cactttttga tgatgagcga
   114001 attttgaagg aggaaacaga atgatccacg aactaaaaat actaccagaa tattttgaag
   114061 cagtgacgag cggacgcaaa caattcgaga tccgcaagaa tgatcgtgac ttcaaagttg
   114121 gtgatcagtt aatactacgt gaatataaaa aatacattaa acatgaatta cctggactaa
   114181 cgagaccaac atttagttac actggtgact catacaaagc agagatcact tatattactg
   114241 attacatgca gaagtccgga tatgtagtgt tggggattaa aggggtgaat taaatgaaag
   114301 tatacatctt agctatcaca gagggcacat ggatgttccc agtcggttcc ggaaaaatct
   114361 ataaatcaaa aacagctgct tataaagcat ttgaaaaata caaaaaagaa aacggtggcg
   114421 gcactaacgc aaagattcta gtggctgata actggcatga agaaggtgaa cgtaattgat
   114481 ttacctgttc attttcgtgg tactaactat ttttatgcta gtaatcttat tcaaaaaaag
   114541 atgagtaaag acgaacgaaa aattctggcc aacttttatt tagtattaaa aatcgaacaa
   114601 aatgtgtttt cggaaaggag aattgtatgg gaaaaacaaa atcaaagatt aagaagaaaa
   114661 aacgacgttt ggaacaaaag gcaatccaga atggaacggc taagaaaaaa taaaaaagct
   114721 acccctttcg agatagcagc gcaatactat tctaccataa ggggtggcgt ttgtgagatt
   114781 tcattggtta aaagattacc aagaactaga tgagcagatt ctttacttaa agtggaatct
   114841 taataaaagt aagcttgaat tgaatcgatg ggtctgtggt gatttagcaa acgtccgcat
   114901 cgaaaagaat tcgagaactt cttctttaga agagaatatc caaaagatag aaaatgaatt
   114961 ggaattgttg atcgagcaaa aagaagaaat gttattgctg atagatagct tttctggtat
   115021 cgataatcaa attgttaaga tgaaatatgt tgatcaaatg agcttagaag atattgctga
   115081 atctgttggg tatagctcat cgtatgtcag acagcgacat gcagaaatta gaaagacatt
   115141 gaacttttta gatgagtatg aacacagaca agctgaccga ttgaaaaaag aaaacgaaat
   115201 tgatttttac aatagcgaga agtacaaaga acaactctct ttattctaaa attacactgt
   115261 tcactaaatg ttcggtcatt gtagctatgt aaacattgtt agcagcatga tattctatta
   115321 gtgtcaaaaa aatatgaaag agccaagata tcccaaccgt tttattaatt ggtatctgtg
   115381 gctcttttat tcttttaatt agtagttggc ataaagttat ttccatttat aaattgatca
   115441 gctaaatcag catcataatc agccgcgata aattgcgctg ctgatttaat aaatgcatcc
   115501 atatcatgaa catctttatt agtatgtcta cgaacatagt gcgtttcatc attaccaatc
   115561 caagtagcag ctttggctaa tctttggagt ttaggaaaat cattcaaata attttcaatt
   115621 gttttaccta ataacatttt ttttatttta tcttcatcct ctgaattttt agatattgca
   115681 taatctttaa ttagaaattc tagagctttt ctataaccaa ctccagcaat ttgttccaat
   115741 tgctcagatt tagcttgtgt ggcttgagta taaatatcaa caaatgtagg agaaactttt
   115801 tcaatatttt ctggtaaatc tattttaatc ggcggacggt aataatcttt aattaaaata
   115861 ttttcagaat atccttcatc ttcattaaac gtatttataa attgtaaact ataaaatttg
   115921 ttgcactcat catttataca ctgtactaat acgccaaaga ttctatcatt atcgaacgca
   115981 ctatatgatg aaaccccaaa atacacatac ggagtcatta tatttccgca atgcggacaa
   116041 ctatttggca aataaattgt ctctgtattt tgacgatcac gatacttaat attaacttct
   116101 tgaagcatta aaacacctcg catctttttt ataaatcata ccacaaaata tctgatagga
   116161 gagtaaacaa atgaatgatt ttcatgaagc ggtacttagt attgaagtag aatcaagttt
   116221 ggctaaagtt tataagaaag caattgaagc agagaatagt ccgtacagag aaaattggaa
   116281 tggtaatcat gcacatgtgc aagtggagaa tgatgattac agaactggaa tgaatacgtt
   116341 agttatttct ttattgtcac acactctacc caatctgcag gaaacgattg aatggtatga
   116401 gcgaatgggc gcaaaagtaa tccgtacaaa ttacaaagga gagaattaaa atggcaatga
   116461 tcaaaattag aacatccatt agtggaacag agtattggga ttcagaaaag aaaaggacag
   116521 tagttgttcc gaaagatcaa gaaccaaatt ttgaagtaaa agaagaaaaa ggaattttga
   116581 gtgatggcaa atcatttgtt gctgtcaatg gcgaactcat agctcatagc agtgaaattc
   116641 ttgatagcga tggaaacact gctgctaatt ttgatggaga cgaagctact aatgatcagt
   116701 ccactgaaga tacggatgaa ctggacaata tgactgcaaa agaattgcgt gcatatgcta
   116761 agaaacatgg tattgatatt cctggtgcta tccgtgcaaa aggcgatatc ataaatctta
   116821 ttcgtgaagt agaatgaaat actgtcagtt tgacggatgc acgaacaaaa tagcaaaggg
   116881 tatctactgt actgaacaca agagatcaag cagatcacgc aagaagaagc agcaagcaaa
   116941 gtctgtttat catcatgaga acaaaccatt ctatcgaacg caagcatgga aagatatgcg
   117001 tcaatttatt tatgaaagag aaggtggtca ttgtcagcgg tgtggtcagt tcatatttgg
   117061 aaagagagca cacgtccatc acattgtacc aatcaaagac aacgaactgc ttaagcttga
   117121 tccaaacaat ctcatgctat tatgttcaaa atgtcatcca attgttgaaa acgaaacgga
   117181 agacaaaaaa gtttttcctt cgtatttcaa ttgaagcccc cctattcatt ttcaaaattt
   117241 tttcgcgtgg ggagataggg tagcggggag tcacgcgcat cgttaggtca aatttttcaa
   117301 aaaacaaagg ggggtgtata caaaaatgac gactaaagcg caacgtaaag cgattattga
   117361 tgaaaaagta agtgctgaaa aagctcgtat cttagaaata atgaatttgt ctgatttgta
   117421 caccatcact cttgatccat taatcgaatc atacttggat atttttgaaa tttaccaaca
   117481 caaatattta ttgtggaagg aaaaaggatt tccggagacg caaaaattca cgaataaatc
   117541 aggagctact aatcaatcaa aacatccatt ggcacaacaa gtagaaactt gggcagataa
   117601 gaagatgaaa gctctagatt tattaggatt aacaaacaaa gcaaaaactg gtagactaat
   117661 tgctggagga tcgaccgcaa gaaaagatga agaaattaca cgcccagaag taaagccagt
   117721 agatgaacta gcagcgcaca gaaataaatg gcgtaagaag gcaggtgctg aaaaatgatt
   117781 gaacctggtg taaattatgc tgatttattt gcaaaagaag taagaaagaa acctaagaag
   117841 tatcctaaga ccgttcgttt agcaatagat cgctggtatc agtggaaaaa aagaaaagat
   117901 atttggtttg atgtagatcg cgcgaatgaa atgatggatt gggtagaatc atttattgtt
   117961 cacacaaaag gagaaatggt gggaaaaccg tttcttttag aaccctggga aaaatttatt
   118021 tattcttgga tttatggttg ggttaaagaa aatgaaaaag gccaaattgt acgcgttact
   118081 cgtgaggctt atgtccagat tccaaaaaag aatggtaaaa cattgattgc tgtcggttca
   118141 ttaggatatg caatgtacgg agaaggggcg ttatcagttg attgctatgc ttgcgcatct
   118201 gattttgctc aagcgcaata tgctgccaaa ccttttgcag ttacaatctt aaacaatcca
   118261 atcctacttg aaggaactaa aatatttagg gggccaaagg gaactgtttc tagtattact
   118321 tatgactata ttcatgaaga tatggcttac tcaaataaat ttattgttca aaccaagaat
   118381 attgataata tcgagggatc caatccatat tttgtattga acgatgaatt gcataaacag
   118441 gagaaaatgg aacagtatga caattttaag tctgctcaga tatctttacc acagccattg
   118501 atgtttaata tctcaactgc tgggaaaggt tcgtcgtctg ttggaatgcg tgtatatcgc
   118561 gaagcaaaag aagtgttgaa acgtgatgat aatgattcaa actttgttct aatctatgag
   118621 ccaaataaaa attatgattg gacggataga aaagtctggg aaatgtgcaa tcctaactgg
   118681 ggaatatcag tcgatttatc tgctttagaa tcggcattta aaacagcgca acggtcagct
   118741 cattccaaag ctgaatttct aacgaaacac ttggatgtat ttgtgaacgg cgcggataat
   118801 ttctttgaac aggatcaagt agagccatgt ttagttacca cacaagaact tggtgattta
   118861 agtggcgaac catgttatat cggtttagat ttatcacgta cacgagattt gacctgtgta
   118921 tctttaaact tcccaacatg ggatgaagat ggtaaagcgg ttcttaaagt aaagcaactt
   118981 tattttattc cgaatgaaga tttagagttt cgagaaaaag aagataatgt tccttacagt
   119041 gatttggctg aacaaggttt cgttgaattt tgcgacggaa aaatgattga tcaagatcag
   119101 attttgcagt acatagagga ctgcatgaat ttatatgata ttcagcaagt gaattatgat
   119161 ccagcgatga gtgacaaact tgttgagaaa ttagaaaact taggattgga atgcgttgaa
   119221 gttgctcaat atcctaaagt attgaatgct ccttttgacg atgtcgaacg gttgttttat
   119281 gagaaacgaa ttcaatttga taatccatta ttcctttatt gcactttgaa cgttgtagca
   119341 attactaaca tcaatggaca aaaagcgcca agtaaacgtc aatcaaagaa aaagattgat
   119401 gggttcgtgg cgtttttgtg tggtcataag gaaacgatga atcaaatgac agatattgat
   119461 tcagatgagt tagatgatta cctaagttcc atctatagat aaatagaaag gcggtgaaaa
   119521 aaattgaaat tacgtgatcg gttatcgaat gcagtctatt cttttatgga aaaacgtgga
   119581 tatatcgagg atatttttgg acactacaca cgttatggtc aaagatatgt gacggattca
   119641 tctatcatgg aatcttctga tgtttatgaa ttagttcaag acatatctaa tcaggtggca
   119701 ttggccacac caattgttat tggtcccgac ggcaacgaag tcaaagatca tcacttacta
   119761 aatattttga agagtcccaa tgattatttg acaggattcg aatttaccaa attggaaaca
   119821 aatactttat tgatcaatgg tgagacattc ccattaacgg atagggatca gcttcatttg
   119881 gcgtacggtg taacaactaa aatcaatgaa cgacttcaag aagaatttga aatgaatggt
   119941 caaaaaatac ctggtcaaat gattcgacac attaagaaca tcggaactga ttcattaaaa
   120001 ggtgctggaa taattgatct tgcaagaaac actctagaag gcgttctgag cgctgaaaaa
   120061 gttttgacgg acaaatatac taaaggcggt ttacttgcgt tcatgcttaa actagacgcc
   120121 cacatcaatc caaataatag cgcccaaaca aaaattgtca aagctatatt ggatcaactg
   120181 gaaggaacgc aaaatgagag tgatcattct gttaagatga ttcctttggg aaaaggatat
   120241 tccatcgaga cattaaaaag tcctgttgat gacgcggcaa ttttgaatta tttaggtgtt
   120301 tacaaaaaag acttaggaaa atttctagga atcaatgttg atacgtatca atcgctgatg
   120361 aagacagata ttgaaaaagc gatgatgtat ctgcacaaca aagcaatcaa accaatattg
   120421 aaaaacaaga gcgaacatta caccgctctt ttttttatgc ctaattctgg ctatagagtg
   120481 gaatggaaaa ttaatatttt ggattttgtt ccttactcaa caaaaacaaa tattggctac
   120541 aacatcgttc gtaccgggat tacaagtcca gataacgtgg cagaaatgct tggttttcct
   120601 aaacagaata ctccagaaac acaagctatc tatatttcaa atgacttatc taggattggc
   120661 cagaaaaatg caacagatga ttccttacca acgaatgatc aagacttgaa aggaggtgat
   120721 ggaaatgaag aagaaggaaa ttcgcacgat tgacatcacc aatctttcaa cacgttccga
   120781 tgaagaaact catacgagga ccattagcgg ctacgctgct gtattcaaca gtcaaacact
   120841 tttatgggat gacttaagcg aagtgattgt tcctggtgca ttctcaaagg caattagtaa
   120901 ttcggatgtt cgttgtttgt tcaatcacga ttggtccaat gtgctaggac gtactaaaag
   120961 cggaaccctt cgccttgaag aagatgaacg tggtttgaaa ttcgaagttg atttaccgga
   121021 cacaacggta gcaagggact tagttaaatc tatggaacga ggagacatca accaatgtag
   121081 ttttggtttt gtgccgactg aagaaacgtg ggactacaat tctgaaccaa tgcttcgaac
   121141 aatccatgaa gtggaattat acgaggtttc tattgtgcct ttgccagcat acgaagatac
   121201 agaagctgca ttaagaagtc gtgatgaatt agaaaaaaac gtcgaagaaa gaaaaaaatt
   121261 aatcaaaaaa attaatcaag cgctagaagc gtaggaggaa aaatacatgg ataaagaatt
   121321 attgaaaaaa atgaaggcac gtcgtgagca acgattgact gaattacgtg aaaaagttga
   121381 atcaggagaa ttacgcgaag cagatttaga agccgtgaaa gaagaaattg acagtgttat
   121441 tgatgaattg aacggaatta aagacgaatt aggcgcagat tctggaactg atgaaacaga
   121501 cgacaataca gatgatcaat cgaatagtac ggattcagac gaaagccgtt ctggtgaaga
   121561 caacgatcaa gaagaagatt cagacagtga agatagttca gaaaatcgtt ctggaatgat
   121621 tactcaacag caacgagatg gattacttgg atcaattaag aacggattgg aggcacgtgc
   121681 aaaaatgacc aagaaacaaa aagatcaaca actacgaaaa gcatttgcta attttgtagc
   121741 tggaaatatt tctgaagcag aagctcgagc tttagggatt gaagctggca acggttcagt
   121801 tactgtccca gaagtaattg catctgaagt tattacttat gctcaagaag aaaatttact
   121861 tcgtaaatac ggaacagtgg tgcgaacatc aggagatgtc aaatatccaa ttcttgtgaa
   121921 gaaagcagat gcgaatgtaa acaagaaaga gcgttcaact gatattgctg aaacagctat
   121981 tcagtttgat gaaattttgc ttaatcctgc cgaattcgat gctttggcaa cagtaactaa
   122041 aaaattatta aaaatgtctg gtgttccagt tgaagatatt gttgtggaag aattgaaaaa
   122101 agcttatgtg cgtaaagaaa tcaattatat gttcaatggt gatgacgctg gaaatgaaaa
   122161 tcctggtgca ttagccaaaa aggctgtagc atttaaaaaa cctttagatc taactgcttc
   122221 aggtgctggg caaaaattat atgatgcatt aatcgaattt aaaaatacac cagtgacaga
   122281 agtgatgaaa aagggacgct ttattattaa tcgagctgct ttgactgcca ttgaaaaaat
   122341 gaaaacagat gatggatttc ctttgttgcg gccattcaca caagcagaag gtggaattgg
   122401 ttaccaatta gttggctatc ctgtggattg gacagatgca gcagataaaa agggtgaacc
   122461 agacacgcca gttttatatt ttggcgattt ttctgcattt aaaattcaag aagttattgg
   122521 tgccttggaa attcaaaaac ttgttgaaaa attctctggt aaaaatcaaa ttggattcca
   122581 aatttacaac ttgctagatg gtcaattggt ttattctcca tttgaaccgg cagtatatcg
   122641 ctacgaaatt acaaaaccag ttggtggtta agatggaaga gcaaactaaa gaattgtctt
   122701 tagaggaaaa attcaaatca catattcatt ttgaagaggg catggatgat tctttgctct
   122761 ctttttattt aaatatggca aaagattatg tcaaaacagc aactggtggc caacaagaat
   122821 atcttatttt gatggttgcc ggcattgcct atgaatatag agtttcagag gatgaactcg
   122881 acaaagctat gaatgccatg acgccattta tcgtgcaagg agcgattcaa aatgccgaag
   122941 agacagacca ataatctcag atggaaagcc gaattgctag acatcaaaac aggaacagac
   123001 ggaaacgatc gtccaactac aatttacgaa tttaagcgtc taatattcta tgaagaactc
   123061 ggtgtgactt ctcaagaaaa atatttatca cagcaagcca agacagacgt tgtcagacga
   123121 attaaagtca gatgggataa atccatcaca gagaaattaa gtgcgctcaa aattgattct
   123181 gtaacgtata acattactcg catttatacg aatcccgatg caagagaaat ggagttgagt
   123241 ttagcttatg tcgattagct ttgatgaatt gaaaacagcg ctgaaatcaa caaagttagc
   123301 agtgttcaga gacaaagcca gattagggac gatgtatcca tacattgtgt actcaaatgt
   123361 gagtaacagc aaaaaaatgg catccggtaa agtctataaa aaattacctt attaccaagt
   123421 ctcttttttt acacttggaa cagaacagga tttagttgtt attgaacaag cgctgcaaaa
   123481 tgctggcatt ccatattcag attttacagg tatacaaggt gatgagaacg acgataccgt
   123541 aaccaactat tacacatatg tgaggtgtgt ggaaaatgcc aagtaataag aatggttttt
   123601 ctgaaatatc ggattattta ggaaaccttt caaaagttga tccgaaaaaa ttatcgttgg
   123661 aatctttaga agaagcagct aacttttatc taaagcagtt gcttcctaat attcctaagt
   123721 cgctcttaaa aaagaaacat atgcgcgatc aaataaaagt agtagttgaa aacgatcaag
   123781 tgaaagttca atttgaagag acggcctttt actggcgctt tgccgaaaat ggcacaacaa
   123841 atcaacgtgc ccaacatttt gcaagtggta cgtatgaaca aaacaaagaa aaaattgaag
   123901 aaattatgac tagaaaaata cttgatttat gggaagggtg attttaattg ggaaaacaag
   123961 atacttttta ttttgaagga ttggacgaca ttttaattgc gatgatggca acgccagatt
   124021 ctgttggaac tgcaccgact tatagtgaag ttttacgatt gccaattgcc acaaaaatcg
   124081 gtgtcaaagg gaatggaaca gcattagaaa aatgggcatc cagcaaaatg tttcgtcgtg
   124141 tgtcgcgaga aacaaaacat gaacttgcgt tggatcacgt gggtattccg atagaagtga
   124201 tggatgaaat caaaggatta gttgctaaaa gcggagtaac gtttagcaaa aataccgcac
   124261 gagaatttcc gtacttcgcc tttggattta ttggaaatat tgaaaatggc ggtaaaaaag
   124321 cagtttggta tcctaataca caactatcca atgtgattga cgaagagtat gccactgcag
   124381 aggaagaaac aaaaattgac gatgtaactg caaatctggt ttccatcgga ctgaaataca
   124441 ataatgtgat gtatgcaagt tttgattcaa atagagacgg agcttctcta gaattattta
   124501 ataaattcat tgctcaacct gtatacgatg aagaacagtg gaaaacattg gctaaagttg
   124561 gaggtgcggg ttaatggctc ggttatctga ttatggaatt cacgttgaag acttaaaaaa
   124621 ttctgctgtt gtaattattc aaggcgcaga attccctatt tcatttacta tgcaaacgat
   124681 ggaatttata gcagatgtgt atggtggaga ttattcgcaa tttgaatctg atatgaacgc
   124741 catgctatct aaaaaagaag gacaaatttc ttctgctaac ttatcgccta gtgatctaaa
   124801 aatcatgcgt gccttgattt atgccatgct gcgtactggt ggtttagagg aagatccaga
   124861 aactattttc aaatttttgg gaatgagtgg agaggtgttg tctgcttata gtacctgtat
   124921 ggaaattttt gctagccaga catttcaggt ggaagaccta aaaaaatcca agaagccaca
   124981 agactttcaa aaagcgcaag caaaaagaaa gaaaaacaaa aagaatcgga agagatagga
   125041 actccttgga gtttttatat ttacgttgct ctcactctat tgaattggag tgagagtttc
   125101 tttttaaagt ctacacctaa cttgtggctc aaatcttatt tacagtggtt acaacaaaac
   125161 accgattttg aaccacctca atctgtaact atggataaat cgccttggtg gtagaaagga
   125221 gcgctaagat aaatggctgg taaagaatct gatgtcgttc ttaattttaa aacgaatggc
   125281 gaagtcagtt attcgaaaac aatcaaagaa atcaacaaag aaatgaactt agccgctgcc
   125341 gagtacaaaa accaagtgtc tgcgatggac aaggatgcaa ctcaaacaga aaaattgcga
   125401 gcggctaaac aaaaattaga aaaacaatta ggattggctg aacaacgatc tcaaatgctg
   125461 agagaggaat atgaaaaatc tgtcaaagaa accggtgaat attctgctga atcagaaaaa
   125521 ctttataaac aattactcaa ttctgaaacc ggagaaaata aacttcgtac agcattagaa
   125581 cagacgaacg atgcacttaa agaacaaggt gacgtttctg ttgatacagc aaaaaaactc
   125641 cagaaaatcg aagaaacagg tgagaaagta aaaggcgttg gtgaaaaaat gtctgttgga
   125701 gtaaccgcgc ctattgtagc ggcaggagca gcaggacttg cagcatttgg tgaagttgac
   125761 gaggcacttg ataccatcat tacaaaaacc ggagcaacag gtgatcaagc tgatagactt
   125821 tcacagtctt tcaaaaacgt tggttcaaat actcatttac ctttacaaac ggttggggaa
   125881 gctattggtg aggtaaatac acaatttgga ttcatggata aaaaactgga agattcaacc
   125941 aattatctcc tacagtacgc tgaaatcaat gatacagatg tttcgcaatc agcaatatct
   126001 gctcgacaag ctattgatgc ttatggacta gaatatgatg atttgaattc tgtccttgat
   126061 gtaacaacga aaacatcgca gaatactggt caatctgtag acgacttgat gcaaaaagca
   126121 attgatggcg caccacaaat taaacaatta gggttgagct ttggtgaagg ggtcacttta
   126181 cttggacaat ttgagcaatc tggtgttgat tcaagcgcag ctttaagtag tttatctaaa
   126241 gcaacagtgg cttatgcgaa agacggaaaa tctttaagtc aaggactaga agaactacaa
   126301 gacaaggtaa aaaatgcagg ttctgaaaca gaagccatca atgcagcagc agaagtattc
   126361 gggactaaag gcggtcctag aatggccgat gcaattcgta gaggtacttt gaacctggaa
   126421 gatttagcaa aaactgctgg agaaagtggg ggagctgtag gagacacatt cgatgctact
   126481 ctcgacccaa ttgatcaagc cgatcaagct atgaacaatg caaaattagc aatggctgac
   126541 gtaggtgaag cagtccaagt aagccttttg cctttttttg aaaaagcaac ttccttatta
   126601 caggaattct ctaaatggtg ggggtctcta gatcaagata caaagaattg gatcataaca
   126661 attgctggta tagcggcagc agttggacca gtactaatcg ttcttggttc acttatggga
   126721 tctgttacaa aaatcgttgg ggaagttaaa agttttatag gagtttggca aggacttgct
   126781 ggtttatttg gaatgtcggg aggctggttt gcattagcag ttatagctat tggcgcatta
   126841 gttactggac taatttgggc atataataac gttaaatggt ttcatgatgg agtgaattct
   126901 ttcttcaaag gtgtttcaga tgtagctgtt gaaatattta attttttaag tggttatttt
   126961 ggtggatttt tcgatggtgt tatagcaaat ttcaataatt tttataatgc tggaaaacga
   127021 attttcagtg gttttctaga ttttatcact ggagtattta cagggaattg gtcaaaagca
   127081 tggcaaggtc tagttgatat ttttggaggt attttcgatg gtattgttgc tgtagggaaa
   127141 gctcctatta atgccatgat tggattgatc aatggattta ttggtggatt gaataatata
   127201 aagataccaa aatgggttcc tggtgtaggt ggaaaatcat tttctatttc aaaactgcct
   127261 tatttagcac aaggcggtca tctaatcaat ggacaagcga ttgttggtga agctggacct
   127321 gagttactga ctgccaagaa tggtaaaacc acagttactc cattatcaga cgaagaaaaa
   127381 cgcaaaggga tcagtggaag agtttctgga ggtagcattg aacagcatat acacattggc
   127441 aaagtcgatg caaacaatcc atctgagtta gatcgtatga atcgtaaatt tgctaaagca
   127501 aacaaacagg ctattcgtga tttgggaggt gttcctattt gagtcgacaa tttatgaatc
   127561 cagatgaacc aaattttatt tggaaaaatc ggaatgcagt aattgatatg aattgcatca
   127621 ttgaggcaga gcttccagat attatgccga acaagcgata cgaaacttac acgattcagg
   127681 gccgaagcgg agaattgacg gagacgtttg atgactatga accttttgat ttggaaatag
   127741 aagggatcac tattccacat tcgaaactga gagaagtcaa acgatggctc actggtaaaa
   127801 gccaattaat cacacataat gatccagaca agtatctaga ggctatctgc agcatggata
   127861 aagaagtccc tttcgaaaac gagtggggct tcttttatac attcgatgtt acttttcgtt
   127921 gtcagccact caaaagaaaa atcggtgaag cacctaaaat ttttcattct agtgttatgg
   127981 atatttttga tcatggtgac gaaattgcac atccctatct ggaaatagaa tcgaacggag
   128041 gagatataac gatcgctgtc ggtgataaaa gcctaacaat acttaatacg ttagctgcaa
   128101 caatcgtggt tgatactcag cttggaaaag caatacaaga aggtttgaat ttatttacgc
   128161 gtggtgattg gccagtattg cagcctgaat ggaatcaggt aaaaatatcc ggaaaattta
   128221 aagaagtgcg gttttggaat aggagtgtgt atctgtgacg caagaattta tttatgctta
   128281 taaaaaaatg ccagatgatt tgagcgtgaa cggagcgtcg ttagttgatt gggaagattt
   128341 gccagaaatc aatcgtgtgc tgaatggcca atatcgtttt tatggtaact attcaagaag
   128401 cggacagtat cgctcttatt taaaaaaagg aaattttata aaagcgaagg taccagatgg
   128461 atcatggcaa tattttgaaa tttataatgt caaaaagaat ttgacctccg tttctgtaac
   128521 agctcggcat attggtttta tggccaataa gaactttatt gtcaagtcgt ttacggataa
   128581 cggcaatggg tctcagatca tgaccaatct taaaaacagt ttggcatttg atcaaaaatt
   128641 taactatctt tcgaatgtag ggacaacgca ccaatttaca gcaagacaag tcgcgccagt
   128701 tgaagcaatc attggttcaa ataacggtaa tcaaaatttg actggcgtga ctagcgcgga
   128761 acttgatatg gataactatg atttgaaact ggtaaaacag attggatcag ataatggttt
   128821 tcggattgat tttggcatca atttagaagc aatcgaagaa gaaattgatg aagaatcaat
   128881 tgtcaatagt ttgtatttgg ttggcggtgt tcctgataac gattatgatg aagacaaaga
   128941 acctatcgaa tatggttatt tagaaattga tggagtaacc aacgagaatc gacgtattgc
   129001 caaacgtgaa aacggagatt gtaaaacagt tgatgaattg atcaaatggg gcaaaacctt
   129061 atttgacaat gatcgtatcc atgagccaaa agctactcat acagtcagta tggtggcgtt
   129121 ggaacacaca ctcgaatacg gtgacatgta tcaagagcta gcgtctttac actttggtga
   129181 tgtcgctcat gttagggcga aagagctaga tatcgagata aaagagcgta tggtcgaata
   129241 cacttatttc ccaacgcttg ggaaatataa ggatattgtt ttaggtaatg atttatcact
   129301 gtacacttcg gcagtaaata ctcaagcaca agaattgaaa aagaaaatcg acaatcgaac
   129361 agaaacgtta gttcaaaatg tgttgaatgc gacggcatgg atcactggta attctggtgg
   129421 gcatgtcgtt tttcgtccag aaaaagctcc atcagagatt cttatcatgg atactccaaa
   129481 tgtggcgaat gcaaagcgag tgtggcgttg gaatttaaat ggcttaggtt attcagacaa
   129541 cggagtaaat ggcccgttcg gtattgctat cacatcaaaa ggagaaattg ttgctgactt
   129601 catcaaagta ggaacaataa atgcagaagt attcgaaact tcctttaatg cttatggtga
   129661 tgtgttaaaa cttgtaaaag gcacgcttca aatttggaat gaaaacaaaa aaatcatgga
   129721 gctaaccaaa aaaggtatgg aattctggaa tagaaaagaa tccattggaa cgatcggaac
   129781 aacagattca gcaggaaatc catttccaaa tgctgtaaca cctacacctc ttgaggaaaa
   129841 ttctttggta atacgtacaa atggcgatgg aaaatacatt ttaatttctc caactgcaga
   129901 aaaaggattt gtgttattag gaaacggaaa agcatattat ttcggtgatt tagatattca
   129961 gggaaaactt actgttagag gtaaagaagt gataccagga caaaatggcg gtccatctgg
   130021 cggaggagaa acacctggtg ggtatcctga tgaattaaaa acagatgcag aaaagagagc
   130081 ttggagaata tacgatattt tgtgtaataa cggatttacg aaacaatctg catgtggaat
   130141 attggggaac attcaacaag aaacaggagg aacttttgat cctgatactg ttcaaatagg
   130201 tggaccagca tacggattag ttcagtggga tggttcttca tatcctcttg ttggtccagc
   130261 aacatgggat ggaaaagttt atgttcaaaa cttatttaat gctgcaggta ttaaagaacc
   130321 aataacgagc ttagatgcac aagttcgatt gcttatttgg acatttacaa atggacaatg
   130381 gatgggcgta gtacaaccta cgacggttga tggatttaag gcctgtactg atccaagaca
   130441 agcagcatat gcttttgaac gaaactatga acgtccggca gcgacacatc ctgaacgtca
   130501 agattatgca gttaactggt ataacaaatt taaagattta aaaccaggag gagctactgg
   130561 agaagcagga ctaaaacatt tagagtcttt gattggacaa cgaattggca atggtcagtg
   130621 ttatggcttg tctgcagaat attcaggata tctgggtggt tgtggcatgg gtgctggaac
   130681 aaaatatggt ttaactcatg tgataggaaa tacttctgca gcatctgata ttggtattgc
   130741 ctatgattgg tctgctgttg gttggaaagt gattcaaaac cctagatatg atcaattagt
   130801 agttggtgca attattaatt gggcaagagg tggacaagtg ggttcatggt ttgcagacgg
   130861 aacttatgga catactggcg ttatccgagg cttagctaat ggtcgtatgc aaacttatga
   130921 acaaaacaca gagttaggta tgatttgtgg aaagttagat cgccagtatt atagtgcaag
   130981 tgcaatttct tccattgtca taccaccaaa atagggaggt gatttaatgg caaagtggaa
   131041 tgtcatatta agtacaacag aaccctataa ttacgtgggg atgattcaag ttcgacaagg
   131101 caataaaaac actgaagtta tggaagctac aattgtcgaa aatggtcttc catatgattt
   131161 atcagaatgt aaggtatatt ttgaatcagt tgttggcgga aaatatccag tccaattaga
   131221 aacaagaatt gtggatgcta aaaaagggaa aatcaa
//
LOCUS       DAFEZB010000002        81386 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFEZB010000002
VERSION     DAFEZB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.002"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..411
                     /locus_tag="LP859_000153"
     CDS             <1..411
                     /locus_tag="LP859_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL3028141.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="LP859_000154"
     CDS             509..697
                     /locus_tag="LP859_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL3028142.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="LP859_000155"
     CDS             complement(755..1108)
                     /locus_tag="LP859_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028143.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="LP859_000156"
     CDS             1639..2061
                     /locus_tag="LP859_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3028144.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="LP859_000157"
     CDS             2058..2288
                     /locus_tag="LP859_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028145.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="LP859_000158"
     CDS             2786..2986
                     /locus_tag="LP859_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL3028146.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="LP859_000159"
     CDS             3013..4206
                     /locus_tag="LP859_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3028147.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSGNKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="LP859_000160"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="LP859_000160"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL3028148.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="LP859_000161"
     CDS             complement(5898..6821)
                     /locus_tag="LP859_000161"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL3028149.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="LP859_000162"
     CDS             7017..7622
                     /locus_tag="LP859_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3028150.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="LP859_000163"
     CDS             7930..8946
                     /locus_tag="LP859_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL3028151.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="LP859_000164"
     CDS             9087..10388
                     /locus_tag="LP859_000164"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL3028152.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="LP859_000165"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="LP859_000165"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL3028153.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="LP859_000166"
     CDS             11117..11515
                     /locus_tag="LP859_000166"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL3028154.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="LP859_000167"
     CDS             11581..12663
                     /locus_tag="LP859_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028155.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="LP859_000168"
     CDS             12875..14437
                     /locus_tag="LP859_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028156.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="LP859_000169"
     CDS             14430..15563
                     /locus_tag="LP859_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028157.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="LP859_000170"
     CDS             15563..16519
                     /locus_tag="LP859_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028158.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="LP859_000171"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="LP859_000171"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL3028159.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="LP859_000172"
     CDS             17808..18626
                     /locus_tag="LP859_000172"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL3028160.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="LP859_000173"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="LP859_000173"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL3028161.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="LP859_000174"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="LP859_000174"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL3028162.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="LP859_000175"
     CDS             complement(20506..21087)
                     /locus_tag="LP859_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3028163.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="LP859_000176"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="LP859_000176"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL3028164.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22221..22634
                     /gene="rpsL"
                     /locus_tag="LP859_000177"
     CDS             22221..22634
                     /gene="rpsL"
                     /locus_tag="LP859_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL3028165.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22718..23188
                     /gene="rpsG"
                     /locus_tag="LP859_000178"
     CDS             22718..23188
                     /gene="rpsG"
                     /locus_tag="LP859_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL3028166.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23282..25369
                     /gene="fusA"
                     /locus_tag="LP859_000179"
     CDS             23282..25369
                     /gene="fusA"
                     /locus_tag="LP859_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL3028167.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25541..26728
                     /gene="tuf"
                     /locus_tag="LP859_000180"
     CDS             25541..26728
                     /gene="tuf"
                     /locus_tag="LP859_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL3028168.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27135..27443
                     /gene="rpsJ"
                     /locus_tag="LP859_000181"
     CDS             27135..27443
                     /gene="rpsJ"
                     /locus_tag="LP859_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL3028169.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27474..28103
                     /gene="rplC"
                     /locus_tag="LP859_000182"
     CDS             27474..28103
                     /gene="rplC"
                     /locus_tag="LP859_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL3028170.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28131..28754
                     /gene="rplD"
                     /locus_tag="LP859_000183"
     CDS             28131..28754
                     /gene="rplD"
                     /locus_tag="LP859_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL3028171.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28754..29044
                     /gene="rplW"
                     /locus_tag="LP859_000184"
     CDS             28754..29044
                     /gene="rplW"
                     /locus_tag="LP859_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL3028172.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29083..29916
                     /gene="rplB"
                     /locus_tag="LP859_000185"
     CDS             29083..29916
                     /gene="rplB"
                     /locus_tag="LP859_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL3028173.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29959..30237
                     /gene="rpsS"
                     /locus_tag="LP859_000186"
     CDS             29959..30237
                     /gene="rpsS"
                     /locus_tag="LP859_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL3028174.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30260..30607
                     /gene="rplV"
                     /locus_tag="LP859_000187"
     CDS             30260..30607
                     /gene="rplV"
                     /locus_tag="LP859_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL3028175.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30621..31277
                     /gene="rpsC"
                     /locus_tag="LP859_000188"
     CDS             30621..31277
                     /gene="rpsC"
                     /locus_tag="LP859_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL3028176.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31280..31714
                     /gene="rplP"
                     /locus_tag="LP859_000189"
     CDS             31280..31714
                     /gene="rplP"
                     /locus_tag="LP859_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL3028177.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31704..31892
                     /gene="rpmC"
                     /locus_tag="LP859_000190"
     CDS             31704..31892
                     /gene="rpmC"
                     /locus_tag="LP859_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL3028178.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31917..32183
                     /gene="rpsQ"
                     /locus_tag="LP859_000191"
     CDS             31917..32183
                     /gene="rpsQ"
                     /locus_tag="LP859_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL3028179.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32241..32609
                     /gene="rplN"
                     /locus_tag="LP859_000192"
     CDS             32241..32609
                     /gene="rplN"
                     /locus_tag="LP859_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL3028180.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32646..32954
                     /gene="rplX"
                     /locus_tag="LP859_000193"
     CDS             32646..32954
                     /gene="rplX"
                     /locus_tag="LP859_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL3028181.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32982..33521
                     /gene="rplE"
                     /locus_tag="LP859_000194"
     CDS             32982..33521
                     /gene="rplE"
                     /locus_tag="LP859_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL3028182.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33540..33725
                     /locus_tag="LP859_000195"
     CDS             33540..33725
                     /locus_tag="LP859_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL3028183.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33761..34159
                     /gene="rpsH"
                     /locus_tag="LP859_000196"
     CDS             33761..34159
                     /gene="rpsH"
                     /locus_tag="LP859_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL3028184.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34191..34727
                     /gene="rplF"
                     /locus_tag="LP859_000197"
     CDS             34191..34727
                     /gene="rplF"
                     /locus_tag="LP859_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL3028185.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34888..35244
                     /gene="rplR"
                     /locus_tag="LP859_000198"
     CDS             34888..35244
                     /gene="rplR"
                     /locus_tag="LP859_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL3028186.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35265..35765
                     /gene="rpsE"
                     /locus_tag="LP859_000199"
     CDS             35265..35765
                     /gene="rpsE"
                     /locus_tag="LP859_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL3028187.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35780..35959
                     /gene="rpmD"
                     /locus_tag="LP859_000200"
     CDS             35780..35959
                     /gene="rpmD"
                     /locus_tag="LP859_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL3028188.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36003..36443
                     /gene="rplO"
                     /locus_tag="LP859_000201"
     CDS             36003..36443
                     /gene="rplO"
                     /locus_tag="LP859_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL3028189.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36443..37738
                     /gene="secY"
                     /locus_tag="LP859_000202"
     CDS             36443..37738
                     /gene="secY"
                     /locus_tag="LP859_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL3028190.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37800..38447
                     /locus_tag="LP859_000203"
     CDS             37800..38447
                     /locus_tag="LP859_000203"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL3028191.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38638..38856
                     /gene="infA"
                     /locus_tag="LP859_000204"
     CDS             38638..38856
                     /gene="infA"
                     /locus_tag="LP859_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL3028192.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38889..39005
                     /gene="rpmJ"
                     /locus_tag="LP859_000205"
     CDS             38889..39005
                     /gene="rpmJ"
                     /locus_tag="LP859_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL3028193.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39023..39388
                     /gene="rpsM"
                     /locus_tag="LP859_000206"
     CDS             39023..39388
                     /gene="rpsM"
                     /locus_tag="LP859_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL3028194.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39416..39805
                     /gene="rpsK"
                     /locus_tag="LP859_000207"
     CDS             39416..39805
                     /gene="rpsK"
                     /locus_tag="LP859_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL3028195.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39885..40823
                     /locus_tag="LP859_000208"
     CDS             39885..40823
                     /locus_tag="LP859_000208"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL3028196.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40851..41231
                     /gene="rplQ"
                     /locus_tag="LP859_000209"
     CDS             40851..41231
                     /gene="rplQ"
                     /locus_tag="LP859_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL3028197.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41503..42342
                     /locus_tag="LP859_000210"
     CDS             41503..42342
                     /locus_tag="LP859_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3028198.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42318..43187
                     /locus_tag="LP859_000211"
     CDS             42318..43187
                     /locus_tag="LP859_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3028199.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43177..43974
                     /locus_tag="LP859_000212"
     CDS             43177..43974
                     /locus_tag="LP859_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL3028200.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44177..44923
                     /gene="truA"
                     /locus_tag="LP859_000213"
     CDS             44177..44923
                     /gene="truA"
                     /locus_tag="LP859_000213"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL3028201.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45256..47925)
                     /locus_tag="LP859_000214"
     CDS             complement(45256..47925)
                     /locus_tag="LP859_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL3028202.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48163..48351)
                     /locus_tag="LP859_000215"
     CDS             complement(48163..48351)
                     /locus_tag="LP859_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL3028203.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48621..48983
                     /locus_tag="LP859_000216"
     CDS             48621..48983
                     /locus_tag="LP859_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3028204.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49035..50717
                     /locus_tag="LP859_000217"
     CDS             49035..50717
                     /locus_tag="LP859_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL3028205.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50834..52870
                     /gene="recG"
                     /locus_tag="LP859_000218"
     CDS             50834..52870
                     /gene="recG"
                     /locus_tag="LP859_000218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL3028206.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52919..53920
                     /gene="plsX"
                     /locus_tag="LP859_000219"
     CDS             52919..53920
                     /gene="plsX"
                     /locus_tag="LP859_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL3028207.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54042..54284
                     /gene="acpP"
                     /locus_tag="LP859_000220"
     CDS             54042..54284
                     /gene="acpP"
                     /locus_tag="LP859_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL3028208.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54552..55556
                     /locus_tag="LP859_000221"
     CDS             54552..55556
                     /locus_tag="LP859_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028209.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55557..56501
                     /locus_tag="LP859_000222"
     CDS             55557..56501
                     /locus_tag="LP859_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028210.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56501..57463
                     /locus_tag="LP859_000223"
     CDS             56501..57463
                     /locus_tag="LP859_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028211.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57490..58404
                     /locus_tag="LP859_000224"
     CDS             57490..58404
                     /locus_tag="LP859_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028212.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58430..60211
                     /locus_tag="LP859_000225"
     CDS             58430..60211
                     /locus_tag="LP859_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028213.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60559..61245
                     /locus_tag="LP859_000226"
     CDS             60559..61245
                     /locus_tag="LP859_000226"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL3028214.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61265..64846
                     /gene="smc"
                     /locus_tag="LP859_000227"
     CDS             61265..64846
                     /gene="smc"
                     /locus_tag="LP859_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL3028215.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64855..65664
                     /locus_tag="LP859_000228"
     CDS             64855..65664
                     /locus_tag="LP859_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3028216.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65675..66673
                     /gene="ftsY"
                     /locus_tag="LP859_000229"
     CDS             65675..66673
                     /gene="ftsY"
                     /locus_tag="LP859_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL3028217.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66860..69127
                     /gene="gshAB"
                     /locus_tag="LP859_000230"
     CDS             66860..69127
                     /gene="gshAB"
                     /locus_tag="LP859_000230"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL3028218.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69216..69668)
                     /locus_tag="LP859_000231"
     CDS             complement(69216..69668)
                     /locus_tag="LP859_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL3028219.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69818..70768
                     /locus_tag="LP859_000232"
     CDS             69818..70768
                     /locus_tag="LP859_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL3028220.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70761..71720
                     /locus_tag="LP859_000233"
     CDS             70761..71720
                     /locus_tag="LP859_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL3028221.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71717..72472
                     /locus_tag="LP859_000234"
     CDS             71717..72472
                     /locus_tag="LP859_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028222.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72495..73448
                     /locus_tag="LP859_000235"
     CDS             72495..73448
                     /locus_tag="LP859_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028223.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74092..74388)
                     /locus_tag="LP859_000236"
     CDS             complement(74092..74388)
                     /locus_tag="LP859_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL3028224.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74743..75099
                     /locus_tag="LP859_000237"
     CDS             74743..75099
                     /locus_tag="LP859_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL3028225.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75109..76008
                     /locus_tag="LP859_000238"
     CDS             75109..76008
                     /locus_tag="LP859_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL3028226.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            76525..77256
                     /locus_tag="LP859_000239"
     CDS             76525..77256
                     /locus_tag="LP859_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL3028227.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            77401..79428
                     /locus_tag="LP859_000240"
     CDS             77401..79428
                     /locus_tag="LP859_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL3028228.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            79536..80633
                     /locus_tag="LP859_000241"
     CDS             79536..80633
                     /locus_tag="LP859_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL3028229.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            80940..>81386
                     /locus_tag="LP859_000242"
     CDS             80940..>81386
                     /locus_tag="LP859_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028230.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        26674 a        14593 c        17388 g        22731 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtggga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaattgc
    22021 atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg
    22081 tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt
    22141 gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg
    22201 tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat
    22261 caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa
    22321 ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga
    22381 cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa
    22441 ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat
    22501 tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg
    22561 ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa
    22621 aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta
    22681 attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc
    22741 aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa
    22801 ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga
    22861 cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa
    22921 cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc
    22981 agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc
    23041 tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc
    23101 aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa
    23161 ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc
    23221 gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag
    23281 aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga
    23341 tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat
    23401 tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg
    23461 tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat
    23521 cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga
    23581 tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg
    23641 gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat
    23701 cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca
    23761 cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac
    23821 aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc
    23881 tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga
    23941 aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt
    24001 aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc
    24061 agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc
    24121 accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg
    24181 tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt
    24241 cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt
    24301 acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa
    24361 ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa
    24421 agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat
    24481 ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga
    24541 taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac
    24601 aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat
    24661 cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc
    24721 ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg
    24781 tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg
    24841 tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt
    24901 tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga
    24961 tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt
    25021 ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct
    25081 tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg
    25141 tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca
    25201 agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc
    25261 tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa
    25321 atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttaaaac
    25381 cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa
    25441 aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac
    25501 aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga
    25561 ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac
    25621 attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta
    25681 cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca
    25741 cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga
    25801 ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc
    25861 tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg
    25921 tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact
    25981 agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga
    26041 tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga
    26101 aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa
    26161 cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt
    26221 tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg
    26281 tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt
    26341 agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga
    26401 catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc
    26461 tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa
    26521 ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga
    26581 aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc
    26641 aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc
    26701 cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta
    26761 ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta
    26821 ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag
    26881 tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg
    26941 ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag
    27001 acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt
    27061 tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg
    27121 aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc
    27181 gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg
    27241 tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc
    27301 ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca
    27361 ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg
    27421 taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca
    27481 aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa
    27541 ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa
    27601 cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca
    27661 aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat
    27721 tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc
    27781 aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta
    27841 tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc
    27901 ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc
    27961 gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa
    28021 gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca
    28081 aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg
    28141 tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct
    28201 tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat
    28261 taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac
    28321 catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc
    28381 gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa
    28441 aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg
    28501 tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg
    28561 ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg
    28621 ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc
    28681 tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg
    28741 aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc
    28801 aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac
    28861 tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt
    28921 gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg
    28981 caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga
    29041 ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac
    29101 aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca
    29161 tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac
    29221 ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac
    29281 ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac
    29341 cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca
    29401 cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc
    29461 ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg
    29521 aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt
    29581 aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct
    29641 gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt
    29701 aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac
    29761 cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca
    29821 gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca
    29881 gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat
    29941 tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat
    30001 ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg
    30061 tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga
    30121 cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt
    30181 gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt
    30241 gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac
    30301 agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt
    30361 cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa
    30421 agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt
    30481 ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc
    30541 aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga
    30601 aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca
    30661 tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg
    30721 aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag
    30781 ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta
    30841 tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa
    30901 ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat
    30961 tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa
    31021 aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg
    31081 gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc
    31141 ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca acatacggaa
    31201 aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg
    31261 agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg
    31321 cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt
    31381 gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat
    31441 gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta
    31501 tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt
    31561 tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc
    31621 tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg
    31681 tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg
    31741 aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag
    31801 caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca
    31861 tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga
    31921 ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata
    31981 aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga
    32041 actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg
    32101 tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag
    32161 tcgaagaagc agttattatc taataacacg agattttcct atatagattg aatgcaaaat
    32221 ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact
    32281 caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta
    32341 acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa
    32401 aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg
    32461 gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc
    32521 gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga
    32581 tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa
    32641 cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca
    32701 aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa
    32761 atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga
    32821 tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc
    32881 gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag
    32941 ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa
    33001 aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc
    33061 aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg
    33121 ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga
    33181 ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg
    33241 caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt
    33301 cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa
    33361 actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag
    33421 ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc
    33481 gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg
    33541 tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt
    33601 atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc
    33661 gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct
    33721 ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat
    33781 tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt
    33841 acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg
    33901 tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg
    33961 taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc
    34021 ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc
    34081 gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt
    34141 tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta
    34201 tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg
    34261 ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga
    34321 atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa
    34381 ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc
    34441 aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg
    34501 tattgaacgt tggttactca catccagtag aaatcacacc accagctgga gtaactgttg
    34561 aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat
    34621 tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt
    34681 atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa
    34741 ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata
    34801 catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag
    34861 tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt
    34921 caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg
    34981 aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta
    35041 acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca
    35101 gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta
    35161 gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct
    35221 cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc
    35281 caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta
    35341 aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg
    35401 tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag
    35461 atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa
    35521 tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg
    35581 ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta
    35641 aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac
    35701 aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg
    35761 gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg
    35821 acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc
    35881 agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt
    35941 ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac
    36001 ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg
    36061 gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag
    36121 ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc
    36181 gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg
    36241 atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg
    36301 gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga
    36361 aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg
    36421 gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga
    36481 cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca
    36541 tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt
    36601 caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg
    36661 ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc
    36721 tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac
    36781 tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa
    36841 tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg
    36901 tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa
    36961 aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga
    37021 aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa
    37081 atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac
    37141 atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc
    37201 gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt
    37261 tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg
    37321 ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc
    37381 tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa
    37441 ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc
    37501 tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc
    37561 agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc
    37621 acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac
    37681 tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga
    37741 aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa
    37801 tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca
    37861 ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa
    37921 acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg
    37981 atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct
    38041 tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa
    38101 aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg
    38161 tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt
    38221 tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa
    38281 gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg
    38341 aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg
    38401 aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt
    38461 agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc
    38521 agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg
    38581 cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg
    38641 gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg
    38701 tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt
    38761 atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat
    38821 cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag
    38881 gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc
    38941 gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag
    39001 gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa
    39061 acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat
    39121 cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac
    39181 agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt
    39241 gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg
    39301 tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc
    39361 tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc
    39421 agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca
    39481 tatccattct acattcaata acactatcat catgatcact gatactcacg gaaatgcatt
    39541 agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc
    39601 cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt
    39661 tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc
    39721 tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg
    39781 ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt
    39841 cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa
    39901 aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc
    39961 ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt
    40021 tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca
    40081 tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga
    40141 aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa
    40201 ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct
    40261 gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt
    40321 atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt
    40381 caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc
    40441 gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg
    40501 aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa
    40561 ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa
    40621 tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac
    40681 gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag
    40741 tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt
    40801 taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc
    40861 gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt
    40921 tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg
    40981 cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg
    41041 ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat
    41101 cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat
    41161 acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag
    41221 aacttgtcta aaatcacact aataacatca ctttatagat gattctttta gagtgttatg
    41281 atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg
    41341 gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt catgacagat
    41401 atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    41461 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    41521 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    41581 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    41641 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    41701 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatgatt
    41761 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    41821 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    41881 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    41941 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    42001 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    42061 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    42121 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    42181 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    42241 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    42301 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    42361 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    42421 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    42481 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    42541 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    42601 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    42661 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    42721 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    42781 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    42841 gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    42901 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    42961 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    43021 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    43081 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    43141 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    43201 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    43261 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    43321 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    43381 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    43441 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    43501 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    43561 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    43621 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    43681 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    43741 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    43801 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    43861 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    43921 gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg ataaacgttc
    43981 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcaagcagtg
    44041 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    44101 gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac cttttaggaa
    44161 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg gaacaaattt
    44221 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    44281 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    44341 agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    44401 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    44461 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    44521 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    44581 acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    44641 ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct acgaggctaa
    44701 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    44761 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    44821 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    44881 acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta tgatagaagt
    44941 ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat tttgcaaaat
    45001 ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taagaaagag gtcgtgagcc
    45061 tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca tctttgatga
    45121 atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt
    45181 ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac agtctgaaca
    45241 ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc
    45301 tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc agacataaag
    45361 aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa
    45421 gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga atgcgtcttt
    45481 gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt tgtcataagg
    45541 aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag cttttctgcc
    45601 agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt gataggctac
    45661 aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc cataaaaact
    45721 tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt attgaccaga
    45781 aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac tagaactttc
    45841 taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg caggcacatg
    45901 gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag tgacgccagt
    45961 cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat agaatgtaaa
    46021 atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg tcaaattatc
    46081 tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta gtggcaatgc
    46141 attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga agccatattt
    46201 actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc catccatcag
    46261 caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga aacctaattt
    46321 atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga taccactgta
    46381 accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc ggtaaaacga
    46441 gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag gatcagtata
    46501 taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac tgttcgtatt
    46561 gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat tgaatgaaag
    46621 ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa
    46681 tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc cccatgccac
    46741 cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccaccat cgtcttgttc
    46801 tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca gataggcata
    46861 acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca atggaacaag
    46921 ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat ttttcaacgg
    46981 tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac ctgcgtaaac
    47041 gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt tcatgattag
    47101 atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt
    47161 tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa
    47221 agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg tcaatagacg
    47281 ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa aactggataa
    47341 aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca taacacgatg
    47401 aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga tgatctccga
    47461 atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat gcccccagcg
    47521 aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc cgaccttaag
    47581 caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga acaccagtgg
    47641 cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc ctagagaagc
    47701 attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc tggcatgaaa
    47761 attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc caggatagat
    47821 tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac tagctgctgc
    47881 gtagcgtccg ttttctctaa agaatgactt taaatttttt ttcatattta ttttccacca
    47941 aatttctatg aagttgtatt cagtttatca tatccttgtt tttctctgct taaaaagagt
    48001 ctagttttca caaaaaaata aaatttagaa taaataactt cataatttgt tcgtataaca
    48061 aaaataaacg caaaaaaagg gtaggaatca aaagtcccac ccttaaaaat ttttattaaa
    48121 taaaaaaccg ttcactatct gaacggtcca agtatcattc aattaaacgc gttcaacttt
    48181 tcctgatttc aaagcacgag ttgacaccca aactttttta ggttttccgt cgaccataac
    48241 gcgaactttt tgtaagttag gtttaacagt acgttttgtt gagttcatag cgtgtgaacg
    48301 gttgtttcca ctttttgtct tgcgacctgt aaagtaacaa acttttgcca tggtaatttc
    48361 ctcctttgcc ttgatttgga gcaatagaat aattcagctc ataccagact aacatatcat
    48421 atagaatggg aatttgcaag tacattttca tagatacaat tgacaattga gaagaataat
    48481 tgtaaattaa acaaggttat acctgaaatt ttgcaaggtt tttgagtact ttgggtaact
    48541 gccttttatt tcaagttgcg ctatgataaa atatgataga agaaaaatgg gagtttttta
    48601 cccgttaagg aggctcattc atggctgtaa aaattcaaac gtcagctgga accattgaaa
    48661 ttaccaatga tgtaattgca acagttgttg gtggcgccgc gacagatgtg ttcggtgttg
    48721 taggaatggc cagcaaaaat caaattaaag ataatattac tgagattttg cgtaaagaaa
    48781 attactcaag aggagtagtt gtccgccaag aagataatgg tattgctgta gatgtttata
    48841 cgattatcag ctatggtacg aaaatctctg aagtttcccg caacgtccaa gaaaaagtga
    48901 aatacaatct agaggcatta ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag
    48961 gtgttcgtgt actgccagac taggcaggag actggctggt aactcagcag tcgatctgaa
    49021 ggaggattat ctaggtgagt gtaacagaaa taagtgcaag ccagttccag gaaatggtcc
    49081 aggcaggtgc gaatcgtctg caagtaaatg cagaatacgt caattcatta aatgtattcc
    49141 ctgtgccaga tggcgatacg ggaacaaata tgaatctatc tatgactagt ggagctaagg
    49201 cagtagtaga ttcaacctca gaaaaagtag gagaattagc agcaatgctt tcaaaaggat
    49261 tgctgatggg tgcgcgcgga aattccggcg ttattttgtc tcaattgttt cgtggatttt
    49321 ctaagcaaat tccagatgtt gtctcattaa atgcttctga tcttgctgca gccttcactc
    49381 atggagttga aactgcgtat aaagcagtaa tgaaaccagt agaaggaacg attttgacag
    49441 tagctagaga agcagcgaaa gctggagaaa agaaagcaaa aagtactgat gatgtcgttg
    49501 aagtaatgac agctgttgta aaaggagcaa agcgtgcttt agctaaaact ccagacttgc
    49561 ttccagtcct aaaagaagtc ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt
    49621 atgagggctt tttaagtgca ttgaatggag aattccaagc ggatgaaaca tatgaacctt
    49681 ctccagcaga aatggatgat atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa
    49741 tggcgacaga agatatccat tttggttact gtacagaaat catggtaaaa atcggagaag
    49801 gacctacagt cgatagtaca ttcgattacg aagaattccg taactattta gatggtatag
    49861 gagattcttt acttgtggtc aatgatgacg agatcatcaa agtccacgtg catacagaaa
    49921 acccaggaga agtcttgaat tatggacaaa aattcggttc attagtcaaa atcaaagtcg
    49981 acaacatgcg cttgcagcat gagacgattt tggaacacga tgaagaagtg aacgagtttg
    50041 cgaaaaaaga aataccacgt atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg
    50101 tccaagagct gtttgaaagt ttaggcgcta attatgttat cagtggtgga caaacaatga
    50161 atccaagtac ggaagacatt gttaaagcga ttcaagaagt aaacgcagat aaagtgattg
    50221 tcttgccaaa caataagaac atcttcatgg cagctgatca agcagcagaa gtagcagata
    50281 tcccagtagc agttgttcct tcaagaacga tttcccaagg attgacagct atgctttcat
    50341 tcaatgatca agcttcttta gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg
    50401 ttgtgagcgg tcaagtcact cacgcgatca gagatacctc aattgacggt gtagagatca
    50461 aagaaggaga ctttttgggc atgatcgatg gaaaaatcct gatctctaac ccagatatca
    50521 cagcaacagc gttaagtaca ctagaacaaa tgatcaatga ggacacagag atcgtaacga
    50581 tcttggcagg tgatgaagga acagttgaac aagcaaatga atttgctgag aaactaacag
    50641 aaaaacatcc tgatttggaa atcgagatcc accaaggaga tcagcctgtc tatccttatc
    50701 tattttctgc tgaataattt ctgtgtcaga atatctttct aacacagtat tcattcataa
    50761 aaggccttcg gttagtgatc aggtatgatc gctgaccaag tccttttttt gttaaaggag
    50821 ggaggaaaaa tttatgcgtt ctttagatga tccaatcact atgctggcag gagtcggaga
    50881 aaaaagagca gctagtcttg ccagcttggg catccaaacg attgaagact tactgactta
    50941 ttatcctttt cgctacgaag atattcagga aagaaatcta aacgagatcc aggatcaaga
    51001 aaaggttact ttgaaaggaa tcgtagtctc gcctcctgtg atgaatcgat ttggttataa
    51061 aaaaagccga ttgcaatttc gtatgatgca agaacatgat gtgttcaacg tttctttttt
    51121 taatcaacct tatctaaaag aaaaagtcat tttatcggag gaaatcgccg tatacggtaa
    51181 atgggatgca aagcgtaaag ccttgaacgg gatgaagatt ttaggttctc aaacaatgga
    51241 tgacttttcg ccgatatatc acgtcaacaa atcaattcgt caaacgacat tagttgatct
    51301 aatcaaaaaa gggtttcaag aatacggcac attgattgaa gaaaatctgc caaacaacct
    51361 tgtggagaaa tatcgactgc ttgaccgagc ttctgctgta agaagtatgc attttccaaa
    51421 agaccacgaa gaaaaccatc aagccaaacg acgtgtcgtt tttgaagagt tctttttatt
    51481 tcaaatgcgt cttcaaggat tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat
    51541 cctttatgat gtccaaaaat tgaaacaatt tactcaaaag ctaccttttg aactgacaaa
    51601 tgcacaaaaa cgtgtaacga atgaaatctg tcgggatctt cgcagtccgc agcatatgca
    51661 gcgacttttg cagggagatg taggtagcgg aaaaacagtg gttgccgcga ttgcgttata
    51721 cgctaccgtt acagcaggtt tccaaggtgc gttgatggtg cctacagaaa tcttggcaca
    51781 acaacatatg gaaagcttgc aacagctgtt tgatcccaat gaagtaacca tcgcattgat
    51841 tacggggtca acgaaagcaa aagaacgacg tgagctcctt gaaaaactag agcaagggga
    51901 aattaacgtc gtgatcggca cacatgcatt gatccaagat ggagtcgaat ttcagaattt
    51961 aggtctagtt attacggatg agcagcaccg ttttggcgtg aaccagcgga aagtacttcg
    52021 tgaaaaaggc tggcgaccag atgtcttatt tatgacagcg actccgattc ctagaacatt
    52081 agctattaca gcatatggtg agatggatgt atctatcatt gatgaacttc cagctggacg
    52141 tatcccgatc gaaacacgct gggtaagaac acctcaattg gacagtgtac tggattggac
    52201 gaaaaaagaa ctggcaagag gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc
    52261 agaagcatta gatgtaaaaa atgcagtgga aatctatgaa aaacttcgtg aactatttgc
    52321 accaaaatat gaagttggct tgttacatgg taaaatgaag aatcaggaaa aagaagtaat
    52381 catggaagcc ttcaaagaca accagatgca gatattagtt tcgactactg taattgaagt
    52441 aggggtaaac gtgccgaacg cgaccgtcat gctgatcatg gatgcggatc gatttggatt
    52501 ggcacagctg caccaacttc gtggacgtgt tggacgcgga agtgatgctt cctactgtat
    52561 cttagttgct aatcctaaaa atgaactagg tgtggaacgg atgaaaatca tgacagagac
    52621 caccaatggt tttgtactaa gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt
    52681 tggcttccgt caatctggtc tgcctcaatt tgcgattgct gacttggtga cggatggaaa
    52741 tgttttggag gtagccagag aagaggctgc agccctttgg caaataaagg attggcagat
    52801 gcttcctgag tatcagcctt tagcgcaaag cctccaaata gaaaatcagg ataatcgtta
    52861 tttcgattag cagactgtgc tatactttat actgatgacg gaaaaaggag tggaaacaat
    52921 gaaaattgcc gtagatgcaa tgggcggaga tcacgcacct caagcaattg tagaaggcgt
    52981 ggctttagcg cagcaagatt ttccagaagt agaattctta ctttacggaa aagaagcaga
    53041 aatcaagaaa tacttaacaa atgaaaaaaa catcacgatt atccatacag acgaaaaaat
    53101 caatagtgat gatgagccag taaaagcaat tcgacgaaaa aagactgcat caatggtcct
    53161 tgctgcacaa gcagtgaaaa acggtgaagc agatgctgta ttttcagcag ggaatacagg
    53221 cgcgctacta gcagcagggt tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg
    53281 cttgatgtct accttgccag tagtagggaa agaaggtgcc ggtttcgata tgctggatct
    53341 tggcgctaac gcagaaaaca aaccagaaca tttgttgcaa tacggtattt taggttcttt
    53401 ttatgcacgt aaagtccgag gtatcggacg tcctcgtgtc gcactcttaa acaacggaac
    53461 agaagcaaca aaaggtagtg aagtgacgaa aaaagcctat gaactccttc agaatgaaac
    53521 aagcttgaat tttattggaa acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt
    53581 agtggtcaca gatggattta caggcaacgc tgttttgaaa tctattgaag gaacagcaat
    53641 gaatatgatg aatctattaa aatctgctat tctaaatgaa gggatcaaag gaaaaatggg
    53701 cgctatgctt ttaaaagacg gcttacgttc tttgaaagca gaaatggatt attccaaaca
    53761 tggcggtgcc gttttgtttg gcttgaaagc accagtcatt aaaactcatg gagcaacagg
    53821 ccccgatgct gttcgctata cgatccgcca aatccacaca atgcttgaga cagatgtagt
    53881 tggacaatta gtcgaacaat ttgaaaaaag cgaagaatag tatttggata tctgaagcga
    53941 tccgttccat tgaaaaaaag gtggacattc ctgaaaaatg ccagtaaaat tagccatggt
    54001 gtaggtaatt gacaagtgta tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat
    54061 ttaacaaagt agcgaaaatt atttcaaatc attttgagat agataccgat aaagtgacgg
    54121 atgaattgaa catcaaagat gatttaaaag ctgattcaat tagtattatg gaattcgttt
    54181 tagagcttga agatgaattc ggtacggaaa tttcagatga agacgctgaa caaattgaaa
    54241 cagtcggtgg cgcagtagat tatatcagca gccatcttaa ataatttcaa ataaaagcaa
    54301 agaaggataa aaagctgcat gcagcttttt atccttcttt ttttaatctt tttgacacgt
    54361 aaaagtcatt tttctgataa ttctggcgat aaacagaaat tatgttgcaa agtaaagcgg
    54421 ttttctatat aattattaat aattcattac acaaagataa gaataacctt tgttataaaa
    54481 cgatttattc tgtgtaatgt ccttttttat taagaaaaac ttccatttag gaagcagaga
    54541 ggggtattcg attgtccgat aaccaattat tagaagttca gaacctacat acaggattcc
    54601 gactcaaaga tgactattac gatgcagtag atgacgtttc atttacttta gacaaaaacg
    54661 agattttagc aatcgttggg gaatctgggt gcgggaaaag taccttggct actacgatta
    54721 tgggattact tgatcctaat aacacaaaaa ttacaggtga gattttatat aacgatctga
    54781 atttaatcga cctgaacgaa acgctctata ataaaatccg cggaaatgat atcggaatga
    54841 ttttccaaga tccgttgtct gcattaaatc ctctcatgag aatcgaagac cagatcaaag
    54901 aaggactttc ctatcacaca aagatgaatg cagaacagcg tcaagctcgt gcactagaat
    54961 tgctagaaca agtagggatt cctaatcctg cgagagttgg acgccaatat ccccatgaac
    55021 tatcaggcgg tatgagacaa cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa
    55081 tcgtcattgc agatgaaccg acgacagccc tagatgtaac catccaagca cagattcttg
    55141 atttactgaa agaccttcag gaagaaacaa aaacagggat catcttgatc actcatgact
    55201 taggagtcgt agcagaaatg gctgatcgag tggcagtgat gtatggcgga cagttcgttg
    55261 aagtagcagg tgtaaaagaa ctgtttgatc atcctaagca tccttataca cgctctttac
    55321 tgaactctat accacaagaa ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg
    55381 ttccttctct gaaaaacatg cctcgtaaag gttgtcggtt tgcaccacgt attccatgga
    55441 taccagcaag cgatcatgaa gaagagccta tccttcatga agtcgcaccg aaccacttgg
    55501 tacgttgctc ctgctataag catttccact ttagagacga aaaaggagag gtgtagatga
    55561 cagaattaat cacaatcaaa gacctgaaag ttcattaccc tattcgcagt ggattcttca
    55621 atcgagtaac ggatcatgta tatgctgtag atggtgtgga ttttattatc gaaaaaggaa
    55681 agacatatgg tttagtggga gagtctggct ctggaaaatc tactacggga aaagccgttg
    55741 tgggattaga aaaggtaaca gccggacaaa tcatgtatga aggaaaagat gtaacgaagc
    55801 gaagcaaccg caaaaaaatg ggttataaca aggatgtgca gatgatcttc caagattcaa
    55861 tgtccagttt gaatcctaaa aagcgagtgt tggatatcat tgctgaaccc atccgaaatt
    55921 ttgaacggtt aagcgatcaa gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg
    55981 ggatgccgga agatgcgcta tataaatatc ctcatgaatt ttcaggcgga caacgtcaac
    56041 gtctaggagt agcccgtgct gttgctacca atccaaaact gatcgtagcc gatgaacctg
    56101 tatctgctct ggatctatct gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc
    56161 aagaatacgg cctcagctat ttattcattt cacatgattt aggtgtagta aaacacatgt
    56221 gcgacaatat cgcaattatg tacaaaggtc gatttgtaga aatcggtaca agagaagaca
    56281 tttataatga cccgcgacat atctatacga aacgtctgct ttcagctatt ccgcaaatcg
    56341 atgtagaaaa tagggaaagc cacaaagaaa atcgacgaag agtagaacaa gagtatatcc
    56401 aaaaccaaaa agaatactat gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa
    56461 ctacacacaa agtggcgttg aaagatggag gtgcaagcta atgtggaaaa cgattctgcg
    56521 acgtgtttta ttgatgatcc cgcaagttat tattttaagt gttctgatct tcttattagc
    56581 ccaagcgatg ccgggggatc catttaccgg tttgatcaat ccaaatcagg atccggcagt
    56641 gatcgaacag atgcgacaag cagctggctt gaatgaccca tggtatgtcc agtacttccg
    56701 ttggatcgga aatgctttgc aaggcgattt tggtcaaagt ttcttataca aacgctctgt
    56761 tgctgacttg atcggcgaac ggatcatcaa cactttatat ctatcgatct tgacagttgt
    56821 ccttacttat ttgattgctg tgcctctggg gatgcttgct ggccggtatc aaaattcaat
    56881 cttggataag attgtcgtaa tctacaactt ctttagtttt gctgttcctt tatttatctt
    56941 cggcttgatc atgctatttg tttttggtta tcgtttaggc tggttcccaa caagtggatc
    57001 acaaacacta ggtttgcaag gtggatttat cactcaatgg atagaccgtt tgaaatatat
    57061 tctgttacca tcaattaccc aagcattttt ggcgactgcc gtcacaatcc aatatctgcg
    57121 aagtgaagta atcgattcga aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt
    57181 accgacgaat aaagtattca gtcgacatat tttccgaaat gcagcgttgc cgatggcttc
    57241 tcaaatgggg tatgaaatca cttcgttgat tgctggttct gtagttatcg aaaagatttt
    57301 tggttatccg ggagtcggaa aactatttat tgattccatc ggccaacggg actataccgt
    57361 gatcacatcc ttagtattga ttttaggaat cgcgacgcta gtggggacat tactttcaga
    57421 tatcatcatg agtatcgttg acccgcgtat tcggattcaa taaaaccaat cagaaaggag
    57481 aatggatcga tgagtgaaaa aaacaataca gcagttcaag aaagtattcc tcctatgggg
    57541 atacgaatga ttgctcgaga atttaaaaaa gataaaattg ctattttttc attaggtttg
    57601 ctagtggttt tattattagt gatctttatc ggtgcgatgc tgaccgatca agacaaagtg
    57661 atgtatgtta gtatcttcga caaatacgct gcaccaggaa cagtttcgac acaaggacaa
    57721 aagttcattc tgggagcaga tgaaggcgga cgtgatgtat taggacaatt gatcataggt
    57781 gctagaaact ctgttacgat cggatttgcg atcacgatcc ttacttcgat catcggggtt
    57841 ggattaggga tcatctctgg ctactacgga gggatcattg acaacatctt gatgcggatc
    57901 gttgatttta tcatgattct accgatcatg ctgatcatta tcgtcttcgt atcgattatt
    57961 tccagataca atgtctggtc ctttatattc atcatgagtg cattttattg ggtagctaaa
    58021 gcccgattat tccgaagcaa gacattatct gaagcaagaa gagattatgt cagtgcctca
    58081 aaaacaatgg gaaccaatga ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt
    58141 tcattaatca ttactaattt gacgatgaac tttgcggcaa acatcggtat cgagacaact
    58201 ttgactttct taggtttcgg cttgccggca aatgtaccta gtttaggtac attgatcggt
    58261 tatgcaagta acggagaagt cttagtgaat cggcaatgga tctggttacc cgcatcaatc
    58321 cttattttag ttttgatgtt gagtatcaat tatgttggac aagcattcaa gcgttctgca
    58381 gatgcgcgcc aacggttagg ctagtataga agcgaagggg gaaataggaa tgaagaaaac
    58441 gatttttgga tttgcagcgc tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg
    58501 tacagatacc tctggaaaca actctggagg caacggcaat aacaaagagg aattaacttt
    58561 tccaattgca acaaaaaata atgatgatgc aatcgaagac ggcgaattag aagctgcggt
    58621 cgcaacggac agtcagttta aaggattgtt ccagtgggag ttttaccaag attcatatga
    58681 tgcagctttt atggctcctt ctcatgaacc gctatttgca aacgatgacg atttcagaat
    58741 cacagacgac ggagctgcaa cgcttgatct agatgaagat acgaaaaaag cgacaatcaa
    58801 agtaaaagac aacgtcaaat ggtcagatgg tgaagatgta acggcggacg atgtgatttt
    58861 cccttacgaa atcattggga ataaagatta cactggtatc cgttatgacg acactttccg
    58921 taacatcgta ggtatggaag aatatcatga tggaagtgcc gacacaatct ccggaatcaa
    58981 aaaaatagac gacaaaacag ttgaaatcga atataaagaa gtaaacccaa gcatgcttca
    59041 atcgggtggt ggtatctggt catatgcagc accaaaacat acgttagaag gtgtagcaat
    59101 caaagatatg gaatcaagcg atcaagtacg taaaaatcca gtgacttttg gcccttatta
    59161 catgagtaat atcgttgcag gagaatcagt agaatacttg ccaaacgagc actactggaa
    59221 tggtacgcct aaattgaaaa agatcacgat gaaatctgta ccgatggcaa gtgcaactga
    59281 agcattgaac tctaaattat atgatatgat tttccaaatg ccaacagata cttacgatac
    59341 ctataaagaa attgctggct atacaaactt aggccgtgaa caaacttctt atacttatct
    59401 aggattcaag ttaggtaaat ggaatgcaga tacaaagagt gtggaataca atccagattc
    59461 aaaaatggct gataaatcat tacgtcaagc aatgggctat gcattagata ataatgcagt
    59521 aggtgaacgt ttctatgcag gtttgagaag caatgcgact tcattgattc ctccagtatt
    59581 ccagtcatac catgatgatg agatgaaagg attcacacaa gatctagaca aagcgaaaaa
    59641 actattagat gatgctggct acaaagatgt ggatgacgat ggtttaagag aagacaaaga
    59701 tggaaataaa ctaacaatca actttgcttc gatgtccggt ggggaaacag cgcaaccatt
    59761 ggcagattac tatatccaac aatggaaaga aattggtttg gatgtccaat acacaacagg
    59821 tcgtctgatc gaattcaatt ctttctatga taaattagaa aacgatgatc ctgaaattga
    59881 tgtttaccaa gctgcttgga atacaggaac tgatccaaat ccaagtggac tatggggacc
    59941 aaatgctgcg ttcaactaca ctcgttatga atcagaagaa aatacaaaat tgatcaacga
    60001 tatcagttca agcaaagcat ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca
    60061 acaatatgca tttgaagaag catttgcgat tccaacgttg ttccgtaatg aagtattgcc
    60121 agtcaacgac cgtgtcaaag actgggattg gtcttatgat gtaccaaacc catgggcaaa
    60181 tgtttctgtt acagcagatt cacgttcata atcactaaaa atgaccgaaa aagggcgatg
    60241 tcctttttcg gttttttgat atttttctgt ttatttctct aatattcagg tcaaggcgct
    60301 gatttcatga cttctccaat aaacggtgtt caaagtccag tttctcggta ttaagtcaaa
    60361 tttatgcaaa aatatggaaa gccattttca caaaattttt tcttcatact cgaaactaaa
    60421 cgaatttttc acaacttctc gtcaatcact aggattatta aagctttact taccttttga
    60481 ccgaaccatt tcaaatttga caaaaaacca gtataataag gaagtatcga aaaaatatcc
    60541 ataaaaggaa tgtggaaaat ggacaaccag ctgacaaaag aactaaaaga aaactataac
    60601 atcgtttttc acgatctaaa tttgcttgaa caagctttta cccattcatc ctatgtgaat
    60661 gagcatcgca atctgcaatt atcggataat gagcgacttg aatttttagg agatgccgtt
    60721 ttagaactga tggtttctca gtatttattc cgtttatatc ctcatttgcc agaaggaaaa
    60781 ttgacaaaaa cacgagctgc gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa
    60841 tgtcactttg atcaatatgt tttgttgggc aagggagaag agaattcagg aggacgtacg
    60901 cgaccagcat tgctttgcga cctattcgaa gcttttttag gcgcgttata tcttgaccag
    60961 ggattcgaca cgacccatgc attcatcgaa aaagtgattt ttcctaaagt caaagcaggt
    61021 gctttttcac atgagatgga tcataaaaca aaactacaag aagtcttgca aaaatcaggt
    61081 gatgtatcaa tcgaataccg attgatcaat gaagaaggtc cagctcatga acgagtattt
    61141 tggatcgaag tttatgtaga tgatcaattg attggcactg gacaagggaa atcgaaaaaa
    61201 ttagcagaac aagctgcggc ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa
    61261 agctttgtat ttgaaacgaa ttgaaatagc cggttttaaa tcatttgcag acaagacagt
    61321 cattgatttt gaaaatagcg tcacagcagt ggttggacca aatggcagcg gaaaaagcaa
    61381 tattacagag gctatccgct gggtattagg agaacaatct gctaaaagtc tgcgaggcgg
    61441 aaaaatgccg gatatcatct ttgccggttc cgatacccgt aagcagctga atattgcaga
    61501 agtcacagtg attttagaca atagtgatca ttatctgcca ttggagtaca atgagatcag
    61561 tgtaacccgc cgttatcgac ggactgggga aagcgagttt ttcatcaata aacaaagctg
    61621 cagattaaaa gatatccaag aattgttctt agattctgga ttaggaaaag aatcgttctc
    61681 aatcatttct caaggaaaag tagaagcgat tttcagtagt aaaccggaag accgtcgtgg
    61741 gatctttgaa gaagcagctg gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca
    61801 gaagcttttt gaaacagaag acaacctcag ccgcgtccaa gacatcatcc acgaattgga
    61861 agaacagtta actcctttgg ccgcccagag tgaggcagca aaggagtttt tacggctaaa
    61921 agaaacactg actcaaaccg atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa
    61981 agattgggac aacaaacaag cacagcttgc gaaattcaat ctagaattag gcaaactatc
    62041 tgagtcgatc caagaacaag aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc
    62101 cgatcgtctg atcgaaaaaa atcagcaagt attattagat ctttcggaaa aactgaagca
    62161 gacagaagga caaaaagatg ttttgcagga gcggacaaag catacacaaa aaagctcgca
    62221 agaatatcaa acttcattag cagaagcaca aaaaaaggtt aaacattttg aaaagcttca
    62281 agaaagcttg atgaaagcag cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa
    62341 tctcataaaa acacagcaag aattagaaaa ataccaaaaa tcaacgaaag aattattggc
    62401 agaattaaga gatcaatatg ttgatttgat gcaagaacaa gcagctgtag gaaacgaact
    62461 gaagtatcta gaacgtcaat atatacaaga aacagcaaaa agcaagcaga cgttagccaa
    62521 gcaatcggaa gtagaagcat cagtagaccg attgatctta caaaaagaag aattgacaca
    62581 aaagcaggcg cagctaaaat catctttaac tgaaacaaaa gagaagttag aaatgatcca
    62641 acaaaacggg aaaaagttcc aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt
    62701 aatgaatcaa gtccagcagc tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa
    62761 ctattttggt ttttaccaag gggttcgatt ggtcttgcag cataaacagc agctttcggg
    62821 tattgtcgga gcagtggctg aactgatcga tgttcctgca gatttcacgt tagctatcga
    62881 aacagcttta ggtggggcag cccagcatgt catcgttgaa aatgaaaaag atgctcggca
    62941 agccatcacg tatttgaaac agcaaagagg aggaagagca acattccttc cactgacgac
    63001 aatcaaacca agacaattac cggctcatat attgacacaa gcagcagctg tagaagggtt
    63061 tatagggatt gccagtgaac aagtcagtta tcccgatcaa atccaaactg tggttcaaaa
    63121 cctgctagga acgatccttt tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac
    63181 gatccgctat caatatcgag tggtttcctt agaaggtgat gtgatgaatg ccggaggatc
    63241 catgacaggt ggagcgaata aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga
    63301 attgaaacag ttgactagtg aatttgaaga agcagataaa caactccaag cacaagaaaa
    63361 aaaagttcag gagctgcaac aagaaacagc tcgtttagcc gaagaacaag aagttttgcg
    63421 tacacgaggc gaacaattac gttttgaaga acaagaagcg acaaatcagc tacaaaacat
    63481 cataaatgaa cttgaacgtt ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga
    63541 actgcagcag tttatcgaag attacgaaaa gcaaacgaac gaattgaaag acaaacaaac
    63601 agatcttgaa tcgcaacgac aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag
    63661 cgaccagatg gaagccagaa gagcacaagt acaaagtcaa aaagcacaag aacaagcaga
    63721 tcttgctgtc ttgaaagagc agtttaatca tctgcagatc cagctccgcg gtgcccgagt
    63781 ccaaaaagca gaggcaaccg aacgtcaaga agcgatagaa aagcagttgg cgacattgac
    63841 agcagacttt tctgatcatg aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt
    63901 atctgctcag cgagaaacat taaaagccga gttggcaaaa gccaaagagc agcgagatag
    63961 aaggcaaaag gagatcgata agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa
    64021 agcacgactg tcggaacaat ccaaattaga agtccaaaaa gaccgagctg aaatgatttt
    64081 agataatcat ttgacctatc tgcagtcaga atatcagctg acatttgaga aagcttccca
    64141 agactatcaa gaaacaaccg atatcgaaga cagtagaaca aaggtttcat ccttaaaaga
    64201 acagattgaa aaactgggac cagtaaactt aaattcaatc gaacaatacg aacaagtcag
    64261 tgaaagacat acattcttag caacacagcg agatgatctg ttagcagcga aaaaccaatt
    64321 gtttgaaacg atggatgaga tggatgatga agtgcggaca agattcaaag aagtatttga
    64381 agctatccgt caagaattca aaattgtctt tccaaatatg tttggcggcg gtaaagctga
    64441 attagtactg acggaaccaa ctgatttgct gaaaacaggt attgagattg aagtacaacc
    64501 tcctggcaaa aaactgcaaa gtctaagttt gctttctgga ggagaacgag cacttacagc
    64561 catcgcgttg ctcttttcca ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga
    64621 ggtagaagca gcgttagatg aagccaacgt gaaacgtttt ggtcgttact tgagcgattt
    64681 ccaagatgac acacaattta tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga
    64741 tgtactgtac ggagtgacga tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat
    64801 ggaagatatc aatgaagagg cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc
    64861 aaattagtag caatcgattt ggacggtaca ttattaaatg aacaaaagct gatttcagat
    64921 gagaataaac aagcattagc acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca
    64981 ggacgcccct tagctgcgat ggcgcattat ctgcaagaat taggactggt agatgaaggt
    65041 gactacagca tcactttcaa cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg
    65101 gaaaagaaag tgatggaagt atcagatatc caacgcttat acaaactggc tcagcaattg
    65161 gatctgccac tagatgtttt gtctgacaat gtggttttac agctgcctag tgccccaagg
    65221 aagcaatcct tgtacaatgt gttgaataag ctgctgcaat tccaaccagc ggatataagc
    65281 gatattacag atgaatttgt gcttaataag gcagtaatcg cttatgacca agaagaatta
    65341 gatcctaaaa tcaaagaaat accagcagaa ttccatgaaa tgtatgagat catcaaaaca
    65401 agaagtatgc ttttggaatt tatgccaaaa ggtgttacaa aagcttatgg tatttcttta
    65461 ttggcaaaag atcttggttt gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat
    65521 gatttaccga tgatccaata tgctggacta ggcgtagcga tggagaatgc tgtgcctttc
    65581 gtcaaagaag cagcagattt cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg
    65641 atccaaaaat ttgtattaga gtagggggag tcaaatggga ttttttgata aaatcaagcg
    65701 tgcttttact ggcgaagaga aaacagaaga agttgaagta caggagaagt acgacaaggg
    65761 tttagaaaag acgcgtaaaa catttggtga acggatgaat gagttgtttg ccaattttcg
    65821 aacagtcgat gaagactttt ttgaagaatt agaagaaaca cttatcggtg cagatgtagg
    65881 ttttgaaaca gcgatacgaa ttaccgaagc attacgtcaa gaagtgaaac tcagaaatgc
    65941 gaaaaaatcg gcagaagtcc aaaatgcgat catcgagaaa cttgtggatc tctatgaagc
    66001 ggaaggaatc aatgaagtaa atgaactaaa tatccaagaa gacggactaa cagttatttt
    66061 gtttgtcggt gttaatggtg tagggaaaac aacaagtatt ggtaaacttg cccatcagtt
    66121 caaaaacgaa ggtaaaaaag tattgatggc agcagcagat accttccgcg ctggagcaat
    66181 cgaccagtta gttgtctggg gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg
    66241 aggcgatcca gcagctgtcg tattcgatgc tttggaaaga gcaaagacag agcaggcaga
    66301 tgttttacta gttgatactg caggccgact gcaaaataaa gtgaacttga tgaacgaatt
    66361 agaaaaaata aaacgagtaa tccagcggga attaccagaa gcaccccaag aaacacttct
    66421 tgtattagat gcaaccactg gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac
    66481 aacagatgtg accggtcttg ttttgacaaa attagatggt acagcaaagg gcggtatcgt
    66541 acttgctatc cgtaatgaat tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat
    66601 cgatgacttg gagccgtttg atccaaatga ttttgtcgtt ggattgttca aaggattact
    66661 aaaagaagaa taacccatgt ctaaaaagca ggagctgtga caaaagttgc agctccttgt
    66721 ttgaatgatt gtttttaaca tagctgtgtt tttgatgcct tcatttttat acttatttgt
    66781 aagcggttct caaacaattt cgttcttttt cttagacttt ttgattataa ttagattcat
    66841 atgaattgga ggtcacttaa tgatgaattt taagcaatta ttattgcatg tcaatgcgcg
    66901 cccttttatc gaccaagctc gatttggtat agaacgggaa ggacagcggg ttgatcttgc
    66961 aggaaatcta gcaaaaaccg atcatccagc aatctttggc gatcgatcct atcaccccta
    67021 tatccaaaca gattttagcg aaacacaaac agagatgatc acccctgtta ccgattctat
    67081 tcccgaatta tttcagtatc tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa
    67141 agaagagatg atctggccat taagtatgcc acctgcctta ccggaaaaag acgaagagat
    67201 cattattgca aaattaaaaa atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga
    67261 atacgggaag cgaaaacaga tggtaagcgg tattcatttc aattttgaat ttggcgatga
    67321 gttgctaaga acattgttca gccatcagga agaatttcaa gatttttccg aattcaaaac
    67381 agaactttat ttgaaaacag cgagaaactt tatgcgctat cgctggatga tcacttattt
    67441 attcggtgct tcaccaatga gtgagaagaa ctacttttta gatgaatcac atccgcaaga
    67501 acctgttcgc agtattcgca acagtgcgct gggctatacg aatcatccaa atgtgaaagt
    67561 ttcctatgct tctatgaaac agtatttagc agatatcgag cgaatgatcg aagaagggaa
    67621 actttcggag gagaaagaat tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc
    67681 agatttagca acaacaggtg ttcgttatat tgaattgcgg aatatcgatt tgaatcctta
    67741 tgcaagattg ggtatcaatc cagagcaagt tcggttctta caattattcc tgatgtacat
    67801 gttatggaca gaagaaaaag aagactgtga ccaatgggta gcagaaggta cgactcgaaa
    67861 taacaaagta gcactagaac aaccatcaga tcaaacagaa tttcatcaag aaggtagaga
    67921 aattcttgaa ggaatgaagc aaatgctggt tgaattggat tggttggatt ctctttattt
    67981 agtagaggaa gcattgactc aaatggatca tcctgaacaa acgttagcag cgaaactcta
    68041 tcaagaagca cagctatcga gtcagcaaga agttgctgtg gcattgggac atcaatatta
    68101 taaagaaagc catgaacgtc cctatcagtt ggccggtttt cgcgaaatgg aactttctac
    68161 tcagatattt atgttcgatg ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc
    68221 ggatcaattt ttgcgtctgc aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat
    68281 gacaagtaag gacagctata tcgtcccact tatcatggaa aacaaaacag taacgaaaaa
    68341 agtcttaaaa gaagctgggt tccgggtacc aggaggcgca gaattttcat ccatggaaga
    68401 agcggtaaaa gcttatccga gatttgccga gcaggcattt gtgatcaaac ctaaatcaac
    68461 caattacggg ttaggtatca cgattttcaa ggaaggagct agtctggaag attatcaagc
    68521 aggtttagcc attgcttttc gtgaagacag ttcggtttta gtagaagaat ttatgccggg
    68581 aacggaatat cgtttctttg tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc
    68641 tgctaatgtt attggtgaca gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag
    68701 tgaccctttg cgtggaacga atcatcgtgc acctttagaa ttgatccaat taggtgaatt
    68761 agaacaattg atgttgaaag aacaagggtt gacgatcgaa tcagttcccc aagccaatca
    68821 aattgtttat ctaagggaga actcaaatat cagtacaggt ggtgattcga tcgatatgac
    68881 tgatgaattt tctgaagctt acaaaaaaat cgccgtttct gcggtagaag cactaggagc
    68941 taagattagt ggtatcgatt tgattatacc agataaagaa atcgatccta caactgataa
    69001 aaaagcatat gggatcatcg aagcaaattt caatccagcc atgcatatgc atgtttatcc
    69061 ttttgcagga aaaggcagaa gattgacgat gaatgtattg aaattgttat atcctgaagt
    69121 attttaaaag aaaaagcttt tgacagattg tcctatttga tgtcaatctg atcaaaagct
    69181 ttttttgcat gtaaaaattt atgtgttttt tcgttttaaa ataaacgatc ccagaagctt
    69241 tttttctttt ttggtttttc tttttcagct ggcgaagagt tttcttctgg gtattttttt
    69301 tcgatatcaa tcacaggttc atttactgct tcttttgctg ttaagagcaa tccgaattcc
    69361 tcaggatttg cagtgacgta atcgttgact acagtgaaac ggctattgtt tttcgttgct
    69421 agctggatat attggttttg gacagaaagg ggtagagccc cattcaagag tatggaagca
    69481 tctggatgtt ttttgagttc ttttaaaaag ttttctttgt tttttggttg gatcatttca
    69541 gcaacagtca tactaagata gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct
    69601 tcagggttca ctaaaggtgt accataaagt cccttatcca aatggctttt tacatcatct
    69661 tgtgccataa aaaataccct ccttcttctc tttgtctttc ttacgattat agcatattcc
    69721 caataatacc taaaacatat gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata
    69781 agaatgaaaa tcattctcaa ttagaaaggt ggattggatg cgctacggac gactagtagc
    69841 attattagtt attttaagta gtatttcatt gttcatcgga gtatcgaatg tacaattgtc
    69901 agaactgctc cagttagatg ggcaaagctt acaaatcttg ctagtgactc gtttgccacg
    69961 aacgatttgt ttgatccttg tgggagcgac cagtagtatt tgtgggttga tcatgcaaca
    70021 tttgacccaa aataaatttg tttcaccaac gactgcgggc acgatggata gtgcgcgttt
    70081 aggtatttta gtggctatga ttttcctacc tggtgcatcc ttgattgtcc gatccttgac
    70141 agcattttgc tttgcttttg caggaacatt gcttttttta tcccttacgc gattgttcct
    70201 ccagaaaaat caattgatgc ttccgttgat tggagtcatg ttagggaata tcattggttc
    70261 agttgctact ttttttgctt atcaattcca gttggtacaa aatatgactt catggcttca
    70321 agggaatttt gcgactgtga tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt
    70381 gttgctcttg gtttggctat ttgcttaccg atttacgatc attggtctgg gcgaatcgtt
    70441 tgcagtaaat ctgggaatag attaccagaa gatgcagttt ttaggcattg gcttagtatc
    70501 attggctagt gcagtcatgt tgatcatggt aggaagcatt ccttttatcg gagtgatcat
    70561 tccgaatctt gtctctagaa tatatggaga tcaagtgcac aaaacaattg gcgtaactgc
    70621 tttagcaggt agtttgttct tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc
    70681 ttatgaaata ccagtcagtg tgatcgttgg aatcatcggt ggggctatct tcctttatct
    70741 tctgataagg ggacgacgat atgcttaaaa aaaagccatc tatttttatt ggtatgcttg
    70801 ttcttgtttt atttgttaca ggaatgtact tgacgatcca tacatatgga aactggcatt
    70861 ttgcatgtgc attgaggagt aagaaaattt ttgcatttat ccttgtctcg ctagctgtca
    70921 gcagtgccac aatcagcttt cagacaatta cgcataacca gtttttgact ccttcagtat
    70981 tgggacttga gtcgttatat gtccttgtcc aaacggtcct tttcttcttt atcggaggag
    71041 tgacgatgct tcagcagaca ggtacagttt ttttcttatt gaacgttggg ctgatggtcg
    71101 ggctgagtct attgttattt ttctttcttt tgaaaaaaat gagagcgaat ctttttttat
    71161 tactgatgtc agggatgatt ttaggtacgc tttttggtag tgtcagtacc tttttacaag
    71221 tcatcatgga tccgaatgaa tatgatttgc ttcaaggcag attatttgca agctttggca
    71281 atatccagag tcagtatctt ttgcctgctt ctttgatcat tatcggctgc tggtacctgt
    71341 tatttcgata tcgacatgtg ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc
    71401 tcgggataga cacagatcgt tttgaatttt tgattttatg ttgtgtcagt cttttgacag
    71461 ggacatcaac ggctcttgtg ggtccagtta cttttttagg gtttattgtc gcaaatatca
    71521 gttatcgtgt cttttctact tatcgtcatg gtcagctatt tatcggaagc tttctggtag
    71581 ctatgctgtt tctgacagga ggacagctcg cagtagagca gattttcggc tggaatacaa
    71641 cagtgagtgt cgtcgttgaa tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg
    71701 aaaggaagag tatcctatga ttgatttaca aaaggtcaat aaactgtacc atggaaaatc
    71761 agtggttaaa gatgtacagt taacgattcc tgaaaagaaa ttgactgctt ttatcggtcc
    71821 aaatggtgct ggaaagtcaa cggtgttgtc gatgatcagt cgtttgatac caaaagatac
    71881 aggtgaaatc tatttagacc ataacgaagt aaaggcttgg aaatccaatg aattagccaa
    71941 aaaattatcg atcctacgac aatcgaatga aataaatgtc caaatcaccg tgaaagaatt
    72001 agtagaattt ggacgatttc cttatacgaa aggaaaattg acgacaaaag acgaggaaat
    72061 cgttcaggaa tcactggaac accttgggtt agaagaaata gcggatcaat cgattgacac
    72121 tctttcaggc ggtcaactcc aacgtgcgta tattgcaatg gtgctagcgc aagacacgga
    72181 ttatatttta ctggatgaac ctttgaacaa tctagatatg aattttgcgg ttcaaatcat
    72241 gcagatttta aaaaatttag tacaagaaat cgggaaaaca attgttattg ttcttcatga
    72301 tatcaacttt gctgcaagtt atgcagacga aattgttgca atgaaagatg gacagttatt
    72361 tacgcaagga acgacccaag aaatgataaa aaaagatgta ttggatgaat tatacgaaat
    72421 ggacatacgg atttgtgaga ttgaaggccg cagattctgt ctttatttct aaaactaaca
    72481 ttaggagaga agagatgaaa aaattagtag taggtatctt gattctagca tcatttggtt
    72541 tagcagcatg tggaaatagc aacaacacca gtcaggcaga cacaaaaaaa agcagcacac
    72601 aaactgaaac tacattgacg ataacagata gtaatggaga ccaaatcgag gtgccaaata
    72661 atccagaaaa agtcgtcgta ttcgacaatg gttctttaga tacgatggat gctcttggtg
    72721 ttggagataa ggtagttgga gcagctacaa gcagccttcc tgagtatctt tcatcctata
    72781 aaaaagtaga atcagcaggt ggaataaaag aaccagactt agaaaaaatt aatcagcttc
    72841 agccagatct aatcatcatt tctggccgac aaagagattt tcaaaaagat ctaagtcaaa
    72901 ttgctccgac cattttcctt tctttagatg cgaaaaatcc atgggaatct ttccagcaaa
    72961 atgtcactgc tttaggggag atctttggta agcaagaaga agctaaaact caattagagg
    73021 aattatctag tgcaatcgat cagacgaaaa agaaagcaga agctactgac aaaaaagcac
    73081 tagtcacact tgtgaatgaa gggcaattat cagcctacgg atctggttct agattcggtt
    73141 tcatccatga tttatttggc tttgaacaag cagatgatca aatcgaagca tccactcacg
    73201 gtcaaagtgt ttcttatgaa tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag
    73261 acagaacaaa agctattggt ggagacgaca gcaaagatga tatttctgcc aatgaactgg
    73321 tagcacaaac gaatgctggt aaaaatcagc agatcatctc ccttgaacca gatgtctggt
    73381 atttaagcgg gggcggttta gaatcaatga aactgatgat cgaagatgtg aatcaagcat
    73441 ttaaatagca taaataacag aggattaaac tctcatacta tgtaacaata atgtttctta
    73501 cattcttaaa tagcaagagc ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg
    73561 tattgatgat tttacggatg gtttttattt atttgagaaa tcttttttca acgtgaattc
    73621 attatctaca tcatacgggt tgattctaga atggggagtg gcaaaaaata gtgtgatcca
    73681 ttttttcagc atcaagatcg cctcctttga tttgaatcaa aggtacaaag cgaaacttag
    73741 ataaaactta aatttagata tttttatttt gtaccgatct cttctttaaa cgatgttcca
    73801 tctgctgctt ctcggcaata agtccactag actagaaaat atgagaagct attttctgtc
    73861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acgatgttcc
    73921 atctgctgct tctcggcaat aagcccacta gactagaaaa tatgagaagc tattttctgt
    73981 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc
    74041 aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg
    74101 tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag
    74161 cttactccac cgttttaata attgatagaa ataactctct tctgctttga ttttatactc
    74221 gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat aaatatccaa
    74281 tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag aaccctcgat
    74341 attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc
    74401 aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca
    74461 aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt
    74521 tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt
    74581 caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata
    74641 aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat
    74701 ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca
    74761 aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat
    74821 atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca
    74881 tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg
    74941 gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat
    75001 ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc
    75061 ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg
    75121 gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag
    75181 ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct
    75241 aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg
    75301 acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat
    75361 tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt
    75421 ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc
    75481 gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc
    75541 gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa
    75601 aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa
    75661 tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga
    75721 gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc
    75781 tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat
    75841 gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct
    75901 atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa
    75961 catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt
    76021 aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt
    76081 atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg
    76141 atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca
    76201 aaaaaaaata taatatacat tttttattta attataatta atattgattg aagtctaggc
    76261 agtcaagtca cattttgata gataggaggg ggacttacga attattttaa aggctatgac
    76321 agctgtattt ctagattgtg tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg
    76381 ctgataatca tttataacta atcgatacat aggaccaaat ataggtctat gttcttttat
    76441 ttcataagta tcgtaaaaca gaattttgct atggattaat ccaatcgact gatatgttta
    76501 gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg
    76561 atgttattca gcatggtagc aagtacgact tttgtttctg caactgaagt atacccaaag
    76621 gaatataaca cagaaggaac gattacattt gaagcaggag atgagggtgt aactcctcct
    76681 gttgatccag agaatcctga tcccaataaa cctgtcgatc catcagatcc tccaagccct
    76741 ggaacaggag gggcattatc gattgattat ggatcaaaat tcaaatttgg gactcaaaaa
    76801 atttctactg cggataaaac gtattatgca gctgctgatg tgatgaacga tgggtctaga
    76861 aaaccgacat atgtccaagt gacagatagg agaagtacct tatctgggtg gaaattaagt
    76921 gtctctcaac cagaacagtt taaaactgcc agtggggatg aactagttgg agcacagctg
    76981 aaatttacga aagggcaggc tgtttctttg gttgatccaa catatacgcc gcaaacggtg
    77041 aattctgaat taacgttgac accgggagga aacaatacgt tagcaattaa tgctaaatca
    77101 ggaacaggtg tggggacttg ggtctatcga tttggtgcaa atgaaaatga aaatcaagat
    77161 gcggtacagc tttcggtacc cggtaaatct gtcaagttag cgcaacaata ttccactaaa
    77221 ttagtttgga cattagaaga tacacctaac aactagttta ctgaaaagaa ctgttaaaaa
    77281 taaatacgta gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct
    77341 aaatttgttt ggatacgaat agttcatcta ggaatttaac gatcaggagg aaaagatata
    77401 ttgaaaaaga ggaggatcgt gctcatttta agcagtctct tagtgactaa tatctttccg
    77461 atcatagggt actctttgga attgaatcag ttggagcatg gaaatagaag agacgaagaa
    77521 agcaaactca cttcgacatt cgacagttta atagaatcca accagacaca agaaagcagt
    77581 gaggatttaa gtatttcgag tagtggagag accactgaca acgtagaagc tgagggttcg
    77641 gacaaacaag gactagaaga tgcaccaatt tctgcggttg agctgcctga cgatcccaat
    77701 attatttcta tcgataaaat ttttcaaaca cctatcggta ctagcacgtc catattggaa
    77761 aatgggaagt tactgcagtt gaacccagca gaaaggtctc aaagaggagc tatttggtca
    77821 aataagaaga ttagtttact ctcggatttt acatttaaat cttatttata cttaggaaat
    77881 caacgagata gagcgggaga tggcatgacc tttacattga ctggcgatcc tagaatggct
    77941 agtaatccca gtcaagttat tggatcgcca ggtatgggaa ttggctcata ttctactcgt
    78001 tctggccaac cttacattgc gaatgcgctc tcgattgagt ttgatacgta taaaaatagt
    78061 ggttcttctg atcgaatgga tcgagagata actgctgata atagagggca tggacatgtc
    78121 gcttttgtca cgcctaaacc aaataataat aattattctg gagagcatac tggcgtgacg
    78181 gtagctccta catttctttc taatggcaca tggcgcttgt taataattaa atgggacgcg
    78241 gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc actcttctcg gattatacct
    78301 gatctcatta ggcagtttgg ttctacagaa gtaaattggg ggtttacttc ttcaacaggt
    78361 actttttttc aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca
    78421 gctgatgtaa aagtcaacga tagtgattat ggggccatcg ctgaagctgg aggaaacgat
    78481 aagataacta tcagaaatac attgaaggtc gagggggatt ttcttgaata taagaaaatg
    78541 cagactagta tcgacctccc acaagaatta actattcccg agcatatctt gatcaatgga
    78601 aatcaaattg atgccaaaga tatcttgata accggaaatc gagtcacgct aaatctgaca
    78661 gattatttta ttgctggaga tacgttaata atcgaattgg agacaacttt gcaggaagta
    78721 attcctgaaa aagtactcac tatgcatttt gaataccttg aaaacgggaa tttgattgag
    78781 caatcgaatg aaataaacct aaagatcgca aagctgaagg agatagatat ccatgtttat
    78841 tataaagata aagacagtca gattgatatt gctgaaatga aaacaatcac tgggaaaata
    78901 ggagagaagt ataaagaaac tcctattgat attgaagggt atgtttttga ctcggattca
    78961 gcgaatacag aaggcatctt ctccgaaaat acagaggata tctattttta ttacagaaaa
    79021 ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc
    79081 agtagaccat taagtgaatt cggacagttt tcaggtgggt tgaaaattat agatgaacgg
    79141 gcttttggaa gttggaaatt acaattgaaa caagagaaag ttcttacaaa tggagatttt
    79201 gagcttccag aagctctttc ctttgttacc tctgaaaata caacggttat agggacatcc
    79261 gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat
    79321 tctacagctc atcgtggaat cagaatagat attcctgtag aaaaccaaag aatcggaacc
    79381 tttgagggga aactatcttg gatacttgca gatgtcccag gaaattaaca ggaagagaag
    79441 aatataacaa aattattgag tttcaagaag ggcacaggta ccataggctc ttgtgtccca
    79501 cagagacaag attttgttat agtcttctct tacatatgat taaaggagcg attttcttga
    79561 gaccaaaaca acaattattt ataacactcc tagttatttt aggggcactt tttaacaata
    79621 tatcagtgtc agctagtgca tttaattttt cagtgacgcc gataacttca gagaatcaaa
    79681 tcgacaagag aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa
    79741 aagcggaatt gagaaatgat acagaaaaag aagtgaaaat cgatatttct gtgaatagcg
    79801 caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa aaagacatga
    79861 gcctgatctt tgatctggta gattatgtga gctatccaca gatagtgacg ctaaaaccta
    79921 aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa tgagcgattt gatggtgtat
    79981 tggctggtgg gatcacattc caagaaatcc agacggaaaa ggaccaaaca gaaactaagg
    80041 atcaaagctt atcgattgag aatgaataca cctatattat tgctttgttg atgcaacaga
    80101 cactgaacga agttgctccg aaccttactc ttcatgaagt taaaccagat caaatcaatg
    80161 caagaaatgt catcttagca aacgttcaaa atgatcaaaa aacctatatc aatcaagtag
    80221 ttattgaaac aaaaattaca aaaaaaggac attctgaagt tttatatcaa gaggaaaaag
    80281 aaggattgca gattgcgccg aatacaaatt tttcttttcc aactgcttta aatggtcaac
    80341 cgctaacccc aggtgaatat caccttacaa tgactgtttt aggaaatgaa aatgaaaatg
    80401 gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa tgggtatttg
    80461 aaaaagattt tacaatagac ggaaaagtag ccaaggaatt gaacacgacg gatgttacga
    80521 ttaaaaaaga caataattgg atttatatct tagtagcttc acttttctta ttgtttattt
    80581 tattatgcat acttttgttt gttttgagaa aaaaagaaga gaaagaaaag taaggttaag
    80641 ataacaaata gtaaattttt attattgtag gatggttggg ctctttttat aacaatataa
    80701 aatataccat tttattaata aaaatgcata ctagtaaatg atataattct aataacaggt
    80761 ttgatcgcga gaaaggccaa ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga
    80821 tgtatattat acaaacaaga atcgctgaca acagtgactc ggatccagga aatcagtttt
    80881 atccccagct agttggtgaa tgcatggaaa gtaatatacg tagaaagagc tgatgactaa
    80941 tgaattatga gtttttttta agcaaagaag atcacagaag ttattcatta cttaaatatt
    81001 tagaagcaag ctcaaattta tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat
    81061 cgacatttct cttgaaaaaa acgatcgata agctgaaaca tgatttacaa aagttcgatc
    81121 tagagaaaaa tttgcgagta accgtcagtg aattggatat atgtctggaa attaatggaa
    81181 aatgttctag caaagctctg ttatctcgtt atataacgga ttctttatct atgaaaatgg
    81241 tattggcttt ctttcaggaa aaatatatct cagtagaaaa ttttgctaaa cagcatcacg
    81301 tgagttattc tgtggcttat aaagtattac aaagactaaa aagaaatcta aaaaaatatc
    81361 aaattttttt tgaaaagaga aagttg
//
LOCUS       DAFEZB010000003        74257 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFEZB010000003
VERSION     DAFEZB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.003"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(524..751)
                     /locus_tag="LP859_000243"
     CDS             complement(524..751)
                     /locus_tag="LP859_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028231.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(760..1233)
                     /locus_tag="LP859_000244"
     CDS             complement(760..1233)
                     /locus_tag="LP859_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL3028232.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            complement(1873..2046)
                     /locus_tag="LP859_000245"
     CDS             complement(1873..2046)
                     /locus_tag="LP859_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028233.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2463..3029
                     /gene="amaP"
                     /locus_tag="LP859_000246"
     CDS             2463..3029
                     /gene="amaP"
                     /locus_tag="LP859_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL3028234.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3042..3230
                     /locus_tag="LP859_000247"
     CDS             3042..3230
                     /locus_tag="LP859_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL3028235.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3243..3800
                     /locus_tag="LP859_000248"
     CDS             3243..3800
                     /locus_tag="LP859_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3028236.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3832..4074
                     /locus_tag="LP859_000249"
     CDS             3832..4074
                     /locus_tag="LP859_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3028237.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4415..5629
                     /locus_tag="LP859_000250"
     CDS             4415..5629
                     /locus_tag="LP859_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL3028238.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5660..5878
                     /locus_tag="LP859_000251"
                     /pseudo
     CDS             5660..5878
                     /locus_tag="LP859_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6297..6770
                     /gene="dfrF"
                     /locus_tag="LP859_000252"
     CDS             6297..6770
                     /gene="dfrF"
                     /locus_tag="LP859_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL3028239.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6897..7553
                     /locus_tag="LP859_000253"
     CDS             6897..7553
                     /locus_tag="LP859_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL3028240.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7735..8265
                     /locus_tag="LP859_000254"
     CDS             7735..8265
                     /locus_tag="LP859_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3028241.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8811..9053
                     /locus_tag="LP859_000255"
     CDS             8811..9053
                     /locus_tag="LP859_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL3028242.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9050..9520
                     /locus_tag="LP859_000256"
     CDS             9050..9520
                     /locus_tag="LP859_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL3028243.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            9648..10862
                     /locus_tag="LP859_000257"
     CDS             9648..10862
                     /locus_tag="LP859_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL3028244.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            11851..12060
                     /locus_tag="LP859_000258"
     CDS             11851..12060
                     /locus_tag="LP859_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL3028245.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            12172..13785
                     /locus_tag="LP859_000259"
     CDS             12172..13785
                     /locus_tag="LP859_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3028246.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            13820..15031
                     /locus_tag="LP859_000260"
                     /pseudo
     CDS             13820..15031
                     /locus_tag="LP859_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            15054..15980
                     /gene="glsA"
                     /locus_tag="LP859_000261"
     CDS             15054..15980
                     /gene="glsA"
                     /locus_tag="LP859_000261"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL3028247.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            16188..17648
                     /locus_tag="LP859_000262"
     CDS             16188..17648
                     /locus_tag="LP859_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028248.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            17869..19632
                     /locus_tag="LP859_000263"
     CDS             17869..19632
                     /locus_tag="LP859_000263"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL3028249.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            19716..20174
                     /locus_tag="LP859_000264"
     CDS             19716..20174
                     /locus_tag="LP859_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL3028250.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            20283..20966
                     /locus_tag="LP859_000265"
     CDS             20283..20966
                     /locus_tag="LP859_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL3028251.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            21113..21727
                     /locus_tag="LP859_000266"
     CDS             21113..21727
                     /locus_tag="LP859_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL3028252.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            21743..21949
                     /locus_tag="LP859_000267"
     CDS             21743..21949
                     /locus_tag="LP859_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028253.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            22490..23620
                     /locus_tag="LP859_000268"
     CDS             22490..23620
                     /locus_tag="LP859_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL3028254.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            23712..24467
                     /locus_tag="LP859_000269"
     CDS             23712..24467
                     /locus_tag="LP859_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3028255.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            24525..26564
                     /locus_tag="LP859_000270"
     CDS             24525..26564
                     /locus_tag="LP859_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL3028256.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(26673..27824)
                     /locus_tag="LP859_000271"
     CDS             complement(26673..27824)
                     /locus_tag="LP859_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL3028257.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(27978..28508)
                     /locus_tag="LP859_000272"
     CDS             complement(27978..28508)
                     /locus_tag="LP859_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL3028258.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(28607..28957)
                     /locus_tag="LP859_000273"
     CDS             complement(28607..28957)
                     /locus_tag="LP859_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL3028259.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="LP859_000274"
     CDS             complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="LP859_000274"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL3028260.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="LP859_000275"
     CDS             complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="LP859_000275"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL3028261.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(31360..32022)
                     /locus_tag="LP859_000276"
     CDS             complement(31360..32022)
                     /locus_tag="LP859_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3028262.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            32206..33852
                     /locus_tag="LP859_000277"
     CDS             32206..33852
                     /locus_tag="LP859_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL3028263.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            33843..34925
                     /gene="uxuA"
                     /locus_tag="LP859_000278"
     CDS             33843..34925
                     /gene="uxuA"
                     /locus_tag="LP859_000278"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL3028264.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(35033..36586)
                     /locus_tag="LP859_000279"
                     /pseudo
     CDS             complement(35033..36586)
                     /locus_tag="LP859_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            36734..37875
                     /locus_tag="LP859_000280"
                     /pseudo
     CDS             36734..37875
                     /locus_tag="LP859_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(38001..39080)
                     /locus_tag="LP859_000281"
     CDS             complement(38001..39080)
                     /locus_tag="LP859_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3028265.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(39183..39623)
                     /locus_tag="LP859_000282"
     CDS             complement(39183..39623)
                     /locus_tag="LP859_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3028266.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(39680..40090)
                     /locus_tag="LP859_000283"
     CDS             complement(39680..40090)
                     /locus_tag="LP859_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL3028267.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(40270..40704)
                     /locus_tag="LP859_000284"
     CDS             complement(40270..40704)
                     /locus_tag="LP859_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3028268.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(40804..41652)
                     /locus_tag="LP859_000285"
     CDS             complement(40804..41652)
                     /locus_tag="LP859_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3028269.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            42041..43807
                     /locus_tag="LP859_000286"
     CDS             42041..43807
                     /locus_tag="LP859_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL3028270.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            43909..45168
                     /locus_tag="LP859_000287"
     CDS             43909..45168
                     /locus_tag="LP859_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3028271.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            45315..46616
                     /locus_tag="LP859_000288"
     CDS             45315..46616
                     /locus_tag="LP859_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028272.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            46619..47470
                     /locus_tag="LP859_000289"
     CDS             46619..47470
                     /locus_tag="LP859_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028273.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            47483..48298
                     /locus_tag="LP859_000290"
     CDS             47483..48298
                     /locus_tag="LP859_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL3028274.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELKLSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            48449..49615
                     /locus_tag="LP859_000291"
     CDS             48449..49615
                     /locus_tag="LP859_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3028275.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            49845..50492
                     /locus_tag="LP859_000292"
     CDS             49845..50492
                     /locus_tag="LP859_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL3028276.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            50576..51520
                     /locus_tag="LP859_000293"
     CDS             50576..51520
                     /locus_tag="LP859_000293"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL3028277.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            51655..53106
                     /locus_tag="LP859_000294"
     CDS             51655..53106
                     /locus_tag="LP859_000294"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL3028278.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            53127..53963
                     /locus_tag="LP859_000295"
     CDS             53127..53963
                     /locus_tag="LP859_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL3028279.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            53963..54622
                     /locus_tag="LP859_000296"
     CDS             53963..54622
                     /locus_tag="LP859_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3028280.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            54748..55437
                     /locus_tag="LP859_000297"
     CDS             54748..55437
                     /locus_tag="LP859_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028281.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            55443..56399
                     /locus_tag="LP859_000298"
     CDS             55443..56399
                     /locus_tag="LP859_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL3028282.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            56443..56955
                     /locus_tag="LP859_000299"
     CDS             56443..56955
                     /locus_tag="LP859_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3028283.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(57211..57759)
                     /locus_tag="LP859_000300"
     CDS             complement(57211..57759)
                     /locus_tag="LP859_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3028284.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(57848..59029)
                     /locus_tag="LP859_000301"
     CDS             complement(57848..59029)
                     /locus_tag="LP859_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL3028285.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(59265..60773)
                     /locus_tag="LP859_000302"
     CDS             complement(59265..60773)
                     /locus_tag="LP859_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL3028286.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            60932..61123
                     /locus_tag="LP859_000303"
     CDS             60932..61123
                     /locus_tag="LP859_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028287.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            61326..62243
                     /locus_tag="LP859_000304"
     CDS             61326..62243
                     /locus_tag="LP859_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL3028288.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            62236..63305
                     /locus_tag="LP859_000305"
                     /pseudo
     CDS             62236..63305
                     /locus_tag="LP859_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            63453..63971
                     /locus_tag="LP859_000306"
     CDS             63453..63971
                     /locus_tag="LP859_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028289.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            63952..64344
                     /locus_tag="LP859_000307"
     CDS             63952..64344
                     /locus_tag="LP859_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL3028290.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            64420..65355
                     /locus_tag="LP859_000308"
     CDS             64420..65355
                     /locus_tag="LP859_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL3028291.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            65488..66267
                     /locus_tag="LP859_000309"
     CDS             65488..66267
                     /locus_tag="LP859_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL3028292.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            66260..66577
                     /locus_tag="LP859_000310"
     CDS             66260..66577
                     /locus_tag="LP859_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL3028293.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            66821..68431
                     /locus_tag="LP859_000311"
     CDS             66821..68431
                     /locus_tag="LP859_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3028294.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            68588..69436
                     /locus_tag="LP859_000312"
     CDS             68588..69436
                     /locus_tag="LP859_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3028295.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            69667..70146
                     /locus_tag="LP859_000313"
     CDS             69667..70146
                     /locus_tag="LP859_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028296.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            70179..72407
                     /locus_tag="LP859_000314"
     CDS             70179..72407
                     /locus_tag="LP859_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3028297.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            72536..74179
                     /locus_tag="LP859_000315"
     CDS             72536..74179
                     /locus_tag="LP859_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL3028298.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
BASE COUNT        25070 a        12754 c        14646 g        21787 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataatagac gtaaataact tctaaaagtc tcacacatct
      481 atcattcaga ttatttttta tttactgttt gttctacttt attttattct tctggtatag
      541 tattcgagtc atttttatag gtcaacaaat cacctggttg acactccaaa acttggcaga
      601 ttttttcgag agtggaaaaa cggatggctt ttgcttttcc attttttaga atagagatgt
      661 ttgccatcgt aatgcccact ttctcactca attcagtcac actcattttt ttttgagcta
      721 atactacatc taaattaacg ataattgcca tgtcgcacct cacactgtca aatcatgatc
      781 tgtcttaagt aaaatcgcat gttcaagcag tttttccagc acgcttgtta atgtgtatac
      841 tgcaaaagga atgaatacga cagcgattcc taccaacatc aaacctggag catcgtctaa
      901 atccgctact ttcataaatt taggaagtaa gccaaaaaag gcgaaaccaa taaacaaaat
      961 ggttcgttcg atttttttta gtatgtttaa gctttgagtg gaaaaagctt ggttatcttc
     1021 gatttttttc aatagtttca ataagaaata agcaataacg atccctagga gaagggcaaa
     1081 gtaaatgaca gaaacaaata atgtggtctg ccaatcaaaa ttgaatcctt tttcgcttgt
     1141 agcgatctga gtataaaaaa gtacgccgaa caaaataaat acggcactta tacctaaaac
     1201 gacaaatttt aatagcaatg ttttaaattt catttttatc cctcttttat ctttgatagc
     1261 taaaatatag cagaataatt attgtaaaac aactattttt tatcgaaaaa caataaaaat
     1321 atatcgttta taaaaaatga tagagtatga aggtgtggag tgcatttaac agcttttttt
     1381 gattgtaaga aacatttaaa accagttatt ttagattcta tgtgtgtctc ttctataatg
     1441 ctagtttaga tgcctgggat tatattggac aatacagttg gaggattttt tattatctag
     1501 cgaagagaag tgtaagttaa cagaactgta tgagaaaaaa gacaactacg tagtagctaa
     1561 ccgattcatt aattttttca agttcatgtt tatttacgta tcagaatttt tcttattcaa
     1621 tggtttggtg taatcaccac ttatttgtcc tttattgctt atacaatgtt atacaaaagg
     1681 aaaatcaagc attgacgcat ggggtggtac tatctagctg aaatagctta ataagcgcaa
     1741 gtagatgcat tggattagtt tataaatcaa aagtgttaga ggtattaaga agaagtagaa
     1801 aaataaaatg gattgccgaa taaaaagtac cataaatcct aaaacatgtg ccaacagata
     1861 gatggcacat gtttattttt ttcgataatt ttgtaacaaa aatacgaaga tgaaaaagaa
     1921 tagagattcc cataatgtag tagtccaact gaattcttta ctgataccaa ataccatatt
     1981 tcctagattt gatacaatca aatagccaat cgttatgatc aatgaatagt acaatctttt
     2041 catcatagga catacctcgt ttttatttat aagaatagta taaaccaaag aagtgaataa
     2101 gaaaaatgat aatataaaaa taaagtaaga acgcattaaa gtaactaaag ttatttattg
     2161 tattttttaa ttctccgaaa aaatttaata aataaataga atcttgtaat aataactgag
     2221 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
     2281 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
     2341 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
     2401 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
     2461 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
     2521 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
     2581 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
     2641 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
     2701 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
     2761 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
     2821 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
     2881 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
     2941 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
     3001 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
     3061 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
     3121 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
     3181 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     3241 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     3301 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     3361 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     3421 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     3481 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     3541 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     3601 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     3661 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     3721 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     3781 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     3841 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     3901 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     3961 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     4021 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     4081 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     4141 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     4201 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     4261 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     4321 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     4381 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     4441 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     4501 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     4561 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     4621 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     4681 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     4741 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     4801 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     4861 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     4921 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     4981 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     5041 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     5101 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     5161 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     5221 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     5281 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     5341 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     5401 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     5461 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     5521 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     5581 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     5641 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     5701 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     5761 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     5821 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt
     5881 ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat
     5941 attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt tgttgccgtg
     6001 aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc tatggtttgt
     6061 caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata ggctgataag
     6121 taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata taatgcagat
     6181 gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag tttgttaaga
     6241 tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg aaatagatga
     6301 taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt aatataccat
     6361 ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat gtggttatta
     6421 tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg aatattgttg
     6481 tttccaccac aacagagtat caaggagata atttagtttc agttaaatca ttagaagatg
     6541 cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga ctatttaagg
     6601 aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat attgaagatg
     6661 gagatacatt ctttccagaa tttgatatca atgattttga agttttgata ggggaaacac
     6721 ttggtgagga agtgaaatat acgagaacat tttatgtaag gaaaaaatga attgagtaaa
     6781 ttttggattt agggtgtttt cataaatata ttgcttttta tgcatatata aattgttgta
     6841 agacaaattc cagtttgtat ggggcgataa aatcagaatt tgagagagga ttgaatatga
     6901 ataaggaatg gtctgaactt aataaaacaa tgcaggcaca aataaaaaag aaagatactt
     6961 ataagagggg tattgatact ttacttactt tacgaagtca gttaatacaa accttagtat
     7021 ctttcaaaga agaattatgc agagaagatt ttaactcaat ccccttcata aacgctgatg
     7081 gttatcatag taagacgatt gcttattcta tttggcatat tttccgcatt gaagatatag
     7141 ttgtgcatac agtgataaat gaagatgaac aagtattttt tgcaggcaat tatcaagagc
     7201 gtatcaattc acctatcatt acaacaggaa atgaactcat gaaacagcag attgcagact
     7261 tttcaaaaca gcttaatctg gaagaattat atttatatat tttcgaagta tgggaaagca
     7321 ctgagaaaat gcttgaacgg ttatcttatg atgaattgaa aagaaaaata ccgaaagaaa
     7381 gaaaaggata cttagaatcg ttgaatgtag taaacgacaa tgaaaaagca atttggctga
     7441 ttgattattg gtgtaataaa gatatttgtg gactaattca aatgccgttt tcaagacatt
     7501 ggattatgca cacagaagcc tgtttgcgta taaagaacaa gatacattca taggcaaaaa
     7561 tttcagtttg tagaactaag gaaattggaa tttaaggagg aataaagtat ggttagtgat
     7621 tttataagag cagcatttcc atggatttta atgggtttat ttgtagcaat cagttgttcc
     7681 cttatgagta aaaaagagaa ataaattcct gtttgtattt ataaaagaga aaaaatgaga
     7741 aaagtagttt tatttattgc catgagcctt gacggatata ttgcggacgg taatggtgga
     7801 gtagcttggc taaatggtca tggaaatgac aatgaaaaca tcgacactta tacagaattt
     7861 accaaagata tagatactgt cttaatgggg tggaatactt ctcatcaggt tgttactgaa
     7921 ctttctccgc aagaatgggt atataataaa tttacaactt atgtattaac acataaagag
     7981 tgcaattctt cccaacaaat tcattttaca agtgaaaatc ctgttttatt attggaacgg
     8041 ctaaaacaag aagcaggaaa ggatatttgg atttgtggcg gagcaaatct tgttcagcag
     8101 ttggtaagca aaaatatgat tgataaatat tacatttctg ttattcctac tctgttagga
     8161 aatggggttc gtcttttggg caacatagac agggaaataa agttaaggct ttgtaaaaca
     8221 cagacttata acggaataac cgatttgatt tatatgcgta gataactaga gagaaacaga
     8281 aaagctgtta aaccgacgga gcgatccgac cacgttggcg ggaaaatcct taagattttt
     8341 ccgccatgct tgcaaggggg cggttcgctt cggcggttcg gcgagcttgc gagccgttca
     8401 aagcccccgt tatctaacaa ggggggcaca aaaaacaaga aacactatac actaacaagc
     8461 ggaaaacgct gtatttataa ggcaaaaccc catttttaca gtagtgatat aaattgtacc
     8521 tatatcacta tgaaaattga atagtaaata tgagaaagac agttgatttc aaaaaataag
     8581 aaattgattg tcgttttctt ttgcagaaat ttaagtgcag gcaattatgg tagttgtcgt
     8641 aattgctgtc agctcagtgt ttatcactga tggtgtgaaa gatttacggt tggagatgtt
     8701 caataagatt attggtgctt aattattttt attgtagtgc tgatatgaga atgttataat
     8761 tggggtataa aacatacaaa taaatttgct gaaaaatgag gtatcaaaat atggaaaatg
     8821 aattactgga aaacttagga agacttcaca caactgaact tggcgttatt agaattaaaa
     8881 agaacttatc tttgaatgta gaaaatgtaa ttgaatggtg caaagaaaaa attagccttt
     8941 ctaatgcgga aatcacaaga aaaggaaaaa actggtatat aacgattgat aattgtatta
     9001 taacggtaaa tgcgtacagt tataccgtta tcactgctca caaggtaaaa tgataaattt
     9061 ttctataaag gaattaaacg catttgcagt tattgggcag gaagtagaac tgactaatta
     9121 tcaaaaaaag aatattcaga ttagtacaca gttttggaga aagtttaaca gtagtttgaa
     9181 aaaagcgtat ctttcacaat caggaaattg ggtaaaatat gcttttatgg aaagaagaaa
     9241 tggaaaactt tattatttct gttctattcc ccaaagaacc attaccccag agggctttct
     9301 gtataaagaa ataccgtctt ataaatattt ggttgtggag catattggtg ctatggataa
     9361 aatatatgaa acttatggca agatttatga agaaattatt cctagtacat cgtatattcc
     9421 tataaaagat attatcctac actttgaaaa atacgattat cgttttcatt ggaatagtga
     9481 taattcagtt attgaaattt ggatacctat tcaagattag aagcaatttt atatccatac
     9541 acacaaagca gttagtctta ggattgactg ctttttctat acagattttt aatgacaggc
     9601 aattatcaat tatggtagtt gccttttttg attggaggaa ttgaagaatg aatgataaaa
     9661 actttattga agaactaaga caaaagcgtg aggaatacgg agtaacacaa acaaggcttg
     9721 ctgttgcctg tggtatcagc cgtgaatatt acaaccgcat tgaaaaagga aaacagccat
     9781 tgaatgacga attaagagaa atcatagaaa aacagattga gcgtttcaat ccgcaagaac
     9841 cactattctt attgattgat tactttcgtg tccgctttcc tactacggac gcattggcga
     9901 ttatccgtga tgtattacaa ctgaaatcta attacatgct ttatgaagat tatggaaaat
     9961 acggatatga aagcaaatat gtacttggcg acatcaatat catgtgttcc atgcaggagc
    10021 atttaggtgt tttattggaa ctgaaaggca aaggctgtcg gcaaatggaa tgttatctgc
    10081 tggcacagga acgctcatgg tatgacttca tgctggattg tatgacggct ggtggtgtga
    10141 tgaaacggct tgatttggct atcaatgata aagcaggaat attggatatt ccaaagttaa
    10201 aggaaaaata caaggctggc gaatgtatgt cctacttccg tatgcagaaa gattacagcg
    10261 gtacagaaaa atgcggtagc gatttaccaa agaatacagg agaaacctta tatctcggtt
    10321 caacaagtag cgaattatat atgtgtgctt atcagaaaaa ttatgagcag tatgtcaaga
    10381 atggcacaga aattgaagat acggagatta agaaccgttt tgaaatacgc atgaagaacg
    10441 aacgagccta ttatgcggtt gtagatttac tgacctatcg ggacgctgaa cgcaccgctt
    10501 tttctatcat caatcattat gtccgctttg ttgacagaga ggacgacaag ccaaaaagtc
    10561 aatggataac aaatgatgat tgggcatggt ttgtagggga aaacagagag ccgatacgct
    10621 taaccacaaa gccagagcct tacactttac aaaaagcgtt gcattggcta caaagacaag
    10681 tcgcaccaac cataaaaatg gtacaggcat tagacagaga aaatcgtaca acgatactga
    10741 aagatatgat tgagcaggca gaactaaaag acaagcataa acatttatta caattagaga
    10801 aatcaaccat agaagaacgt atagataccg ctgttccaca agagaatgac ggtatttttt
    10861 aatgtaattg aaaaatttca gatttagtca gcaaagagct gttttcattc cctatgtgaa
    10921 gtgaaagaag aaaaataaat tttttattaa atctgcaaca aaatggctac ttgcgtacag
    10981 atatggtgcg agaaaaggaa atctaaactt tttttgaaaa taggtcatta aatcggggct
    11041 tgctgtccct atatgaagtg aaagaagaaa aaagttttga aaaaatgacg gaaattcgtg
    11101 attagtggac agtcttctgt gaaaagagga aaaacatcat cattttgtat tcaaacaggc
    11161 attagcagtt gagtgttgat gtgaaaagat gaaaatactt tgttctcaac ttaacaattt
    11221 aaggtttccg ttccctatat ggtgcaaaga aaatatttca ttctattgtt ggcagtaacg
    11281 gtatggtttg ggtagttagg gtgtgaaaag gaaaatattc ttttttcatc atcaacttag
    11341 caaaatgaca ttttctatcc ctatatggtg catgaaaaga agttaaaatt tttccattta
    11401 aacttagcaa atggacgttt gctgtacaga tagtagtgaa aaaggagaaa atcaaccgtt
    11461 gattgccaaa atgaaccgtt ccatttgtac taataatgga aaaagttttg gattttggtt
    11521 acgtttctcc tcttttccaa cgcgatatat aggaaagacg atttttttct ttcaatcgtt
    11581 ctttgacaac tgaataaagc tattcaatac gtttgacaga cagctagccg ttgaaacaaa
    11641 tacgccacga cctttcccct gcaagagcga gcgaaaacct atttgtgttc ttgtaaaaga
    11701 tttgtcctct tttatcgcca tgacctgtac tgtcagataa tgatactccc gtacagccac
    11761 agcttgagca ttaagagcgt cgcacgcaat gggtacggct acataagaac tatgcagagg
    11821 tggaactcct gtgggctatg acaaaagccg ttgaattgct tagaaagatt ttacagaaag
    11881 ggggtatgtg ttattgataa tgctgtaaaa acaattcaaa agaaaaatgg caagtgtggc
    11941 attggcaaca gaggaaagac aaagattgta gtaaaagagc atttttccga aaagggcaaa
    12001 acaatggaag aacttctaac tgatgtcatg ctggaaaaag caaagcagac aatcgcataa
    12061 aatcggtgca gttgtaagaa atagattgaa tgacaaaaag tacccgtgtt atactttact
    12121 tgcaagcagg tattgtaaac acgggttagt tataaaggag gataactgac aatgaaacaa
    12181 cagatttaca atactgcact ttatcttagg ttaagccgag atgatgaatt acagggcgaa
    12241 agttccagca ttaccacaca gagaagtatg ttgcgtttat atgcaaaaga acatcatttg
    12301 aatgtcattg atgaatatat tgatgacggc tggtcgggaa caaattttga cagaccgagt
    12361 tttcaaagaa tgattgagga tatagaggca ggaaaaatca actgtgttgt aacgaaagac
    12421 ctttcccgtc ttggcagaaa ttatattatg acaggacaat atacagaatt gtatttccct
    12481 agtcataatg tccgctacat agcgattgat gacggtgtag acagcgaaaa aggcgaaagt
    12541 gagattgcac catttaagaa catcatcaat gaatgggtgg caagagatac aagccgtaaa
    12601 gtcaaatcag catttaagac aaagtttgcg gagggggctc attacggtgc ttatgctccg
    12661 ttaggatata agaaacaccc tgacatcaaa ggaaaactgt tggttgatga tgaaacaaaa
    12721 tggattattg aaaaaatctt ttccctagcc tatcaaggtt acggaagtgc aaaaatcaca
    12781 aagcagttaa gagcagaaaa agttccgaca gcgtcatggc taaattttac aagatacggt
    12841 acttttgcac atatctttga gggaaaaccc gaaagtaagc gttatgagtg gacgattgct
    12901 catgtcaagg cgatattgaa aagtgaggtt tatatcggaa acagtgttca caatatgcag
    12961 tctacggtat cgttcaagag caaaaagaaa gtgcgtaagc ccgaaagtga atggtttcga
    13021 gtagaaaaca ctcacgagcc gattattgac aaggaagtgt tctatcgtgt gcaggagcag
    13081 ataaaatcaa gacgcagaca gacaaaggaa aaggcaacgc cgatttttgc agggcttgtc
    13141 aagtgtgcgg attgtggttg gtctatgaga tttggaacga ataagacaaa taaaacgcct
    13201 tacagttatt atgcttgcag ttactacggg cagtttggca aaggtaattg ttctatgcac
    13261 tacattcgtt atgatgtgct gtatcaagcc gtattggaac gcttgcagta ttgggctaag
    13321 gcagtacagc aggacgaaga aaaggtattg aacaagatac agaaagctgg caatgcagag
    13381 agaatacggg aaaagaagaa aaaggcaagt acattgaaga aagccgagaa cagacaaaat
    13441 gagattgacc gtttatttgc gaaaatgtat gaagatagag cctgtgagaa gataacagag
    13501 cgaaattttg tgatgttgtc cagcaagtac caaaaagaac agatagaact ggaacagcag
    13561 ataacaagcc taagagaaga actaagtaaa atggaacagg atatgatagg tgctgaaaag
    13621 tggattgagt taatcaagga atattccgta ccaaaggaac tgacagcacc attattaaat
    13681 gccatgatag aaaaaatcct catacatgaa gcaacaacga atgaggataa cgaaagaata
    13741 caggaaattg agatatatta ccgatttatt ggaagagttg agtaatcaac aagagtaata
    13801 tttttaacta agggaaaccg gcggtgtcta tacttgggtc agtcgcacat tgggaaaacg
    13861 attcggtttt gccgcaatct ttttccaatg gtttgagata acagttggct acttgaccat
    13921 gctgtatttc ttaactggtg cattatccta tgcaacgggt attggtgcta tacaaaacaa
    13981 taaatatctg aaattggcaa tcctattgat tattttctgg gctattttac tctcacagtt
    14041 aaaaggaacc aaatacactt cattgattgc tcggatcggt tttgtagctg gtatcttgtt
    14101 gccagcatta gtcttgtttg gcttagggat ccattatgtc gcttcaggcg ctccactaca
    14161 aacgatactg tctatgaaaa cattgattcc tgatttttcg aaattgccta cgctggttgt
    14221 ttttgtttcc tttattttag catatatggg cgtagaaaca tctgccagtc atgcaaatga
    14281 aatggaaaac cctaaaaagg attatccatt agcaatgttt gtattggtta ttctggcaat
    14341 cattttagat acttttggcg gattgaccgt cgctactaca gtacctcaac atggtttgtc
    14401 tcttaataca ggggtgattc aatcgttaga attcctatta agacacattg atccaaatct
    14461 tgcatggatt gcgaaaattc ttgcgattct tgtttgttta ggtgtcatcg gagaaattgc
    14521 ttcttgggta acctctcctt caaaagcact tcacgtagca gcagaagaag gattacttcc
    14581 tgaatatttt gcaaaagaaa acacacacgg tgttcctgct catttgatga tcgcaaatgg
    14641 gatcgttgca acaatctggg cagccgtctt tacattaagc ggcgggggga ataatctttc
    14701 atttcttgca gcgatgtcat tgactgttgt aatctacctg atgttgtact ttatgttttt
    14761 cgcgggctat ttgaagctgg tttttaaaga tgacagtctt gaaagagcct atcaggttcc
    14821 aggcggaaaa gttgtaaaag taatcgttgc ttgtttaggt cttttgactt cactttttgc
    14881 tttttgtatc tcatttgttc cacctgctag tatcgcagct ggacagcatg ggatttatga
    14941 aacgattctg attgtttctt atcttatcgt cttagttatt ccatttacga tctacgcatg
    15001 ccgtcataaa ttccagcatg cagaaaaata aaaaatgaaa gctggttatt caaatggaag
    15061 taaaagaatt agaacaattg attggaaaaa atttccacta ttatcaaaca ggacaagtgg
    15121 cggattacat tccagcatta gctgaagtgg atcccaaaca attaggtatg gcgatttatg
    15181 atttggatac aaaacaactg attgagggag gagacgccga tgtgcgcttt gccatcgaaa
    15241 gcatgtcaaa agttcccgta ttgttattag caatcaaaga caacggaata gaaaaagtct
    15301 ttcaaacaat caacacggaa ccaaccggtt ttgccttcaa ttctatcatg aatatggaaa
    15361 tcaatcatcg caaacatcca atgaatcctt ttgttaatgc aggagcgatt gcaacaacct
    15421 cgttgattgc cggtaaagat gcagatgaaa aatttgagcg aatcttgtca tttatgaaag
    15481 agatctgtga tgatcctaat atcacattga atgaagaaat ctatcattct gaatcaagaa
    15541 caggtgatat caatcgctct cttgcttact atatgaaagg taatcaaatg atcgaaggag
    15601 acgtaccgga gatactcgat gcttatttca gacaatgctc cctcaatgtc accgctacag
    15661 gcatagcaaa attagctgct gtgctggcaa ataaaggaat tgctccttgg aatggcaaac
    15721 aaatcattac tgaagaaagt gctacaatcg tcaaatcaat tatgacaact gctggtttgt
    15781 atgatgaatc aggagagttt tccgtccatg ttggtgtacc tgcaaaaagc ggtgtaggcg
    15841 gcgggttgat ggcagctgtg ccaaatcgtt acggcatagg tgtcttcagc ccggcactag
    15901 atccatttgg taacagcgca gctggtatcc aattgttgaa agatgtagtc aaagaactag
    15961 acgctgacat ctttgaataa ccattcttga aagaggctgg ggtaaaagtc caaacggctc
    16021 aaagctacct acaatgaggc tgaaattgtg agcttgtttg acttttgtcc cagtttcttt
    16081 ttttattggt ttttgaaagg gcttttctaa ataaaaagga aacaaaaata cgatttacag
    16141 atatgattaa ataaaatcat aagaaaaact ggaggcgtgt atcaggaatg ctggaaaatt
    16201 acatcgagcg aaatatattt agaaaagtgt atctgtgtga acaattattt gaattccaag
    16261 aaatagacat cgaacaaaca gctatttctc taagggtgac tacaccaacg atccttcacg
    16321 atctagaaag tctagccgag tgtctagaat attgtataaa agaacaagta agagagaagc
    16381 ataagtataa actcgtattc aaacatggta ttgctctttc tgagctcaca cagtttctgt
    16441 acggtcaatc ttatttttta aagtttttaa gttattattt taatggtata ttcacgagta
    16501 cagaactggc tgatcttgaa tttatttctt tgagtaaagt atatacaatc aaaaaaatcg
    16561 tattggattt ttttaaagaa aatgattatt tgaaaaataa agaaatcatt atcccagaat
    16621 ttgactcgcg gaacatcttg ctagctttag tacgttatgt caattgggaa ggctatgaaa
    16681 ataaaaatcc gtatgtagaa gaagcttgta acgagttgat tgattatgta gagactcatt
    16741 tttttaaaag aagatattcg gaagatgaaa agtcctttat catacgagaa atagagatcg
    16801 caatcggaag aaaggcattt ccccttggac tgaaagaaga agataagaaa agcgcagaaa
    16861 cgaagccgtt atttcaatta gtctgtcaag gattggaaga gcaaaaagga attctagact
    16921 tacaagaaga agatgtctat tatatttttt ctttgttcaa cagtagaaat tataccaatg
    16981 agaacatgga gctgttaaga aaagatttcg aagttgttta taaaaatttt gttctcaatg
    17041 acctatcact gaatgaattg attttacaga ttattagtat cgctgaaatc gacgatgcag
    17101 aaaattttat tttcaagcaa tcctttttac aatttgtccg tacgttgtgg gcagatggac
    17161 aagtattttt accggaaagg atctatcttc tatcagaaaa agaacaactt ctgtacggaa
    17221 aaattgtaga aatattggaa gagtggaaaa agaaaaatca aatccaacta agatggaatg
    17281 aaaatttcat tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa caagcgggga
    17341 gacaggaaat agaaattttt gttgtttcag aaagttcagt caaacaattt ttttatcgaa
    17401 cccaatttca tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt
    17461 cattagaaga attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg
    17521 ctgctagcta tcaaaatgga ttagaaactg aaaagacaaa gatctatcca atctcattaa
    17581 aaggtgcaaa catccatttg tatcatattg ctcaggaact tcacctatta aaaaaacata
    17641 tttcttgatg agaccttaat taggagacct tctacaaagc tttttaattg tctgtagaag
    17701 gtttttattt gagtcctgat tcgtgcgaag atctacttat ataacaagct ttctctgaaa
    17761 tttttttaaa atgctattga cttagagtca actctaagta gtagaataga taaagtgtaa
    17821 caaatctatt ttcattaaaa gaagatagta cggaggagag aatgtattat gagtacagta
    17881 ttacatgtat tggatgcaat caatagcccc gcagatgtca aaaaattatc tgttgaggaa
    17941 atgaaacagt tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt
    18001 gggcacgttg gtccgaatct aggtgtagta gaattaacga ttgcgtttca ttatgtattt
    18061 gattcaccaa aggataaaat cgtttgggat gtttcccatc agtcttatgg tcataagctt
    18121 ttaactggtc gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag cggatattcc
    18181 aaccaacatg aaagtaaaca tgactatttt acagtggggc acacatctac atctatcagc
    18241 ttagcaacag gtttagctaa agcgagagat ctgaagaaag aatcaggaaa tgtggtagca
    18301 tttatcggtg acggctccct aagtggtggc ttagcttttg aaggattgaa caatggtggt
    18361 gttttacaat ccaatctaat cgtattagta aacgataatg agatgtctat tgatgagaat
    18421 catggcagtt tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat
    18481 aacctattta aagcaatggg atttgattat cgttacgtgg ataatggaaa cgatctggaa
    18541 acggtgatcc gtttatttga agaagtaaaa gatattgatc atccgattgt acttcacgtc
    18601 catacggaaa aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa atatcattgg
    18661 catgtaccat ttgatctgtc caccggggag gcaaagcaag taacttctgg agaaacattt
    18721 aatatgatca tcatggatta tttggataag aaaatgcaaa caagtacagc tcctattgcc
    18781 gcagtcaatg cagcgatacc aggcgccttt ggactgaaaa cattccaaga aaaacatcca
    18841 gatcattatt ttgacgtggg gatcgctgaa cagcactcta tcactttcgc ttctgctttg
    18901 gcttctcagg gagtacgtcc agtcgtcttc caatcaagta ctttcttgca acgggcatat
    18961 gatcaattat cacatgattt agcgatcaac gagaatccag tgattatttt agtcggtggc
    19021 ggaacgatct cagctagtga ccgaacacac caaggaacat ttgacattcc ttacttatca
    19081 agtatcccga acatcaaata tcttgctcca gcaacaaaag aggatatgat ctctatgcta
    19141 gattgggcgt tcgaacaaac agcgcagcct gtagcaatcc gaattcctac acatggtgta
    19201 gaacatggta cagctatcgc aaatgattat agtattccag cctatcaaac agtaaataat
    19261 ggaggaaaag tagcaatttt agctttaggc ggttttcttg aattaggaaa agaaacagta
    19321 aaagctttta gaaaaaaagg aatcgaagca acgttgatca atccaggaac gatcacagaa
    19381 aatgacgtag aaacattaga taaattaaaa gaaacacacg aattagtcat cacattagaa
    19441 gatggtagtt tgtcaggagg atttggcgaa aatatcagtc gttattatgg tgattcttca
    19501 atgaacgtat taaattttgg tgcagagaaa gaattcacgg atagcgttcc tgtagggcag
    19561 ctttatgaac gttatcattt aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg
    19621 aatattcaat aattcattat gctcaccatc cagataattg tttatactag tatctggatg
    19681 gttttttcat tataaaaagc aaaggggtgg atgttatgta tcagcaagta gaagaatatg
    19741 tcaaaaagtt ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt ttcttttcat
    19801 ttggggatac agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa aagataaaga
    19861 ctggtacatc aagtggttac gatctgtata aacaagagaa tgagccttta ccaaaagttg
    19921 gtcaattaga cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa
    19981 atgtgaccat cttgcctttt agtgaagtca cgaaagagca tgcttggaaa gaaggagaag
    20041 gggatcggac attagcttat tggcggaaag tccatgctga gttttttaaa catgcttata
    20101 aagaagcgtt gaacatagaa tttacagaag agaagctggt cgtatacgaa gaatttgaag
    20161 taattttctt ttagtaagaa aggtctgctg aaagaaatag ctcattattg tctcctgaat
    20221 agaaaagggt tacattttta tatgaagctg tatttttata taacattact ggaggagata
    20281 ttgtggatac actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa
    20341 acgagccgct acacagttct tttattatgg ggagaatggc tttggctgca aaagaaggcg
    20401 gagcagttgg tattcgagca aatacgacag aagatatcaa tgaaatcaag cgaatcgttg
    20461 atttaccggt aatagggatt atcaaaagag attacgatgg gtcagaagtg tttattactg
    20521 cgacagaaaa agaagtggaa gaacttttgg agacgcaagc agaaatcatt gcactggatg
    20581 caacttctcg taaacgtccg acagaaaaaa caactgctga acttgtccaa atgattcacg
    20641 aacacggtcg gttagctatg gcagatattt cgacttttga ggaagcgctt caagcgcaag
    20701 aagatggttt tgatcttttg tctacgactt tagcaggcta tacagaatat tcacgaaaga
    20761 cgcagacacc tgattttgat ttattgaatc aaatcataga acaagtcgat atcccagtaa
    20821 ttatggaagg acatactgac tctcctgaac aagtagagaa agcatatgaa ttaggtgctt
    20881 tttcagtcgt agttggatct attatcaccc gtccgcaatt gatcactaaa agatatgtcg
    20941 aagccgcaag taaagcaaat aactaaaaaa cacgcacggc acagattctt gacaaaaaag
    21001 aaaaattgat agatagttta aagcagattc taagaagatg aaaaaagcat tacttttcat
    21061 tttgtcttca agatctgctt tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga
    21121 acggagtcct tcagaattga cagaacgtga aaattacaag ttgctgattg gttcagtcat
    21181 ccctagacca gtagcagtag taacgactaa atcagcaaaa gatatagtga atatcgcacc
    21241 cttcagttac ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg cgatccagag
    21301 aaaacaagat gcagtaaaag acacagcaaa gaatattctt gagacaagag aagctgttat
    21361 ccatatcctt gatgaggaca acgtagaaga agtaaataaa acagcagcca atctgcctaa
    21421 tgaggaaagc gaactgtacc tgactgactt gactttaact gattcagtaa tcatttcagt
    21481 accagcattg acagaagcaa aagttcgttt tgaaacatcc ttatatgaac atgtcgagat
    21541 acggaataaa aataaagtaa cagctgattt atttttgcta gaagtacaga aatatcattt
    21601 aaatgaaaca gtctatgatg ataagacagg aaggatcgat ccaagaaaat tgaatgctgt
    21661 aagccgtttg gcaggaaatg attatgcagc aataggagaa attttcacga ttgcacgtcc
    21721 cgaataaaaa ggagcactag ttatgttaaa aataagctat aaaccatcga cagattcaaa
    21781 agaaatgaaa aaagaatatg aaacagtgaa tgattttctg caagggcagt acttagaagt
    21841 tccgccattg caagaccatt ttgtcgttac tactgttact ttagatggta aggaaataga
    21901 aatgccagat caaacgatta gcggattgtt taattacttt aacaaataaa tatttattcc
    21961 gaaaagtgaa aaaacatata aaaaaacaca taaaactaaa aaattatcaa agaatcctgg
    22021 tgcaactgaa agaatttgca acgggattct ttcaacttat ttgttatttt tttctaaaaa
    22081 atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga
    22141 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
    22201 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
    22261 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
    22321 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
    22381 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
    22441 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt tgggtcctta
    22501 tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt atcgtttatt
    22561 gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca tatttttgag
    22621 ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc accctaaaat
    22681 agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa tagggatatt
    22741 cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc aattccagcc
    22801 ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc gtgacggaaa
    22861 tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa cttttctgat
    22921 tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt gggtcatatt
    22981 ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt tttctgcatt
    23041 gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc ttccacagat
    23101 gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg atagtcatgg
    23161 tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca tatttgggcg
    23221 gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa cagattttat
    23281 tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt
    23341 gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg
    23401 attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga acgtgggaag
    23461 tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct acggaggaac
    23521 aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt caaagataca
    23581 ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg agaaactctc
    23641 agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc ttttagaaag
    23701 aggaattcgc aatgaaagta tctcaaaaaa tcatattatc tattttgtta ctgcttacat
    23761 tagtagttgg ctatttttcg attgagttta tccatggatt ttcgtctgca aaacagagtt
    23821 caactgtgaa aaaggtagag ccgaaaagta tccctaccac actaaatgtg gctttgattg
    23881 gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga ttcacttctg gttgcacaat
    23941 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
    24001 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
    24061 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
    24121 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
    24181 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
    24241 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
    24301 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
    24361 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
    24421 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
    24481 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
    24541 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
    24601 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
    24661 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
    24721 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
    24781 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
    24841 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
    24901 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
    24961 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
    25021 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
    25081 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
    25141 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
    25201 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
    25261 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
    25321 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
    25381 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
    25441 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
    25501 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
    25561 caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa gctggatcaa
    25621 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
    25681 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
    25741 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
    25801 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
    25861 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttatcaagca acgcgtgtca
    25921 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
    25981 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
    26041 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
    26101 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
    26161 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
    26221 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
    26281 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
    26341 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
    26401 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cttgataacc
    26461 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
    26521 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt gctttttaag
    26581 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
    26641 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
    26701 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
    26761 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
    26821 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
    26881 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
    26941 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
    27001 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
    27061 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
    27121 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
    27181 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
    27241 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
    27301 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
    27361 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
    27421 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
    27481 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
    27541 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
    27601 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
    27661 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
    27721 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
    27781 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
    27841 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
    27901 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
    27961 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
    28021 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
    28081 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
    28141 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
    28201 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
    28261 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
    28321 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
    28381 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
    28441 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
    28501 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
    28561 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
    28621 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
    28681 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
    28741 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
    28801 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
    28861 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
    28921 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
    28981 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
    29041 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
    29101 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
    29161 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
    29221 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
    29281 tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
    29341 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
    29401 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
    29461 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
    29521 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
    29581 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
    29641 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
    29701 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
    29761 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
    29821 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
    29881 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
    29941 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
    30001 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
    30061 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
    30121 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
    30181 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
    30241 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
    30301 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
    30361 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
    30421 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
    30481 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
    30541 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
    30601 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
    30661 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
    30721 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
    30781 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
    30841 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
    30901 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
    30961 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
    31021 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
    31081 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
    31141 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
    31201 tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa ttattgtaat
    31261 ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta ctcaaaaacg
    31321 aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga aatactcggg
    31381 ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca atcccgtttg
    31441 aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat aatgttgttc
    31501 catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga aacgttctaa
    31561 atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa tttcgaaaca
    31621 agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc
    31681 tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt ccaatttgga
    31741 acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag cattttctgc
    31801 agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat acacgagttc
    31861 ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag caagatgttc
    31921 ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga taatttgttt
    31981 acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc ctccaaaatc
    32041 atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat gtcacattaa
    32101 ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta ttgcataaaa
    32161 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
    32221 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
    32281 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
    32341 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    32401 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    32461 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    32521 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    32581 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    32641 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    32701 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct actttataaa
    32761 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
    32821 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    32881 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    32941 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    33001 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    33061 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    33121 ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    33181 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    33241 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    33301 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    33361 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa tatgcctcag
    33421 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    33481 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    33541 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    33601 gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg tacgcaagat
    33661 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    33721 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    33781 ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    33841 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    33901 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    33961 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    34021 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    34081 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    34141 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    34201 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    34261 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg ggatgggaac
    34321 ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    34381 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    34441 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttagctcgaa
    34501 tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc ccttataacg
    34561 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    34621 tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    34681 tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca ttagacatgt
    34741 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    34801 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    34861 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    34921 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attcttgata
    34981 cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg gtctaggaag
    35041 taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat tcaaagatat
    35101 attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt ccttttaaaa
    35161 tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt
    35221 tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa atttgttcta
    35281 tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca ccaatcacaa
    35341 ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc atccattgca
    35401 cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg aaatccatat
    35461 aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga taaagcatga
    35521 aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa agtttatttc
    35581 gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa tcaaatacag
    35641 tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt gcttttttta
    35701 aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata gaaagtcgag
    35761 ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt cgcaaaatat
    35821 ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct ttttgtacgt
    35881 ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa tataatttat
    35941 ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa
    36001 aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc
    36061 aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc
    36121 cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca
    36181 ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat
    36241 ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc
    36301 ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa
    36361 aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta
    36421 attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt
    36481 gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc
    36541 tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt
    36601 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
    36661 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
    36721 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
    36781 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
    36841 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
    36901 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
    36961 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
    37021 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
    37081 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
    37141 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
    37201 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
    37261 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
    37321 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
    37381 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
    37441 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
    37501 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
    37561 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
    37621 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
    37681 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
    37741 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
    37801 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
    37861 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
    37921 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
    37981 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
    38041 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
    38101 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
    38161 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
    38221 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
    38281 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
    38341 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
    38401 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
    38461 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
    38521 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
    38581 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
    38641 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
    38701 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg
    38761 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
    38821 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
    38881 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
    38941 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
    39001 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
    39061 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa
    39121 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    39181 tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    39241 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    39301 tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca
    39361 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    39421 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    39481 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    39541 aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga
    39601 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    39661 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    39721 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    39781 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    39841 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    39901 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    39961 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    40021 gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    40081 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    40141 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    40201 gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca
    40261 tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg
    40321 atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac
    40381 accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt
    40441 attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc
    40501 agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc
    40561 atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg
    40621 tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa
    40681 atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta
    40741 gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc
    40801 aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac
    40861 aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg
    40921 atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc
    40981 tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata
    41041 atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat
    41101 gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat
    41161 aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa
    41221 gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt
    41281 tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa
    41341 ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct
    41401 tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat
    41461 aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa
    41521 tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat
    41581 agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct
    41641 cgtttctttc atttattcac ttccttaatt cattaataat actcttatct tattataact
    41701 tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat
    41761 gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga
    41821 aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga
    41881 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
    41941 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
    42001 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
    42061 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
    42121 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
    42181 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
    42241 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
    42301 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
    42361 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
    42421 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
    42481 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
    42541 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
    42601 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
    42661 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
    42721 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
    42781 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
    42841 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
    42901 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
    42961 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
    43021 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
    43081 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
    43141 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
    43201 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
    43261 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
    43321 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
    43381 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
    43441 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
    43501 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
    43561 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
    43621 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
    43681 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
    43741 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
    43801 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
    43861 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
    43921 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
    43981 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
    44041 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
    44101 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
    44161 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
    44221 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
    44281 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
    44341 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
    44401 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
    44461 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
    44521 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
    44581 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
    44641 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
    44701 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
    44761 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
    44821 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
    44881 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
    44941 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
    45001 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
    45061 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
    45121 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
    45181 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
    45241 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
    45301 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
    45361 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
    45421 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
    45481 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
    45541 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
    45601 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
    45661 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
    45721 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
    45781 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
    45841 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
    45901 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
    45961 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
    46021 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
    46081 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
    46141 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
    46201 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
    46261 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
    46321 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
    46381 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
    46441 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
    46501 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
    46561 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
    46621 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
    46681 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
    46741 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
    46801 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
    46861 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
    46921 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
    46981 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
    47041 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
    47101 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
    47161 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
    47221 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
    47281 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
    47341 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
    47401 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
    47461 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
    47521 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
    47581 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
    47641 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
    47701 ccaaaaattt tctgaactta aattatctga aactgggtta caagcatcgc aacaaacgat
    47761 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
    47821 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
    47881 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
    47941 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
    48001 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
    48061 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
    48121 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
    48181 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
    48241 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
    48301 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
    48361 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
    48421 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
    48481 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
    48541 cggagatacg gacactccta cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
    48601 agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc atcgaatcga
    48661 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
    48721 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
    48781 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
    48841 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
    48901 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
    48961 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
    49021 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
    49081 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
    49141 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
    49201 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
    49261 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
    49321 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
    49381 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
    49441 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
    49501 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
    49561 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
    49621 gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt
    49681 gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt
    49741 ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta
    49801 tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta
    49861 tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag
    49921 gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct
    49981 caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc
    50041 agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag
    50101 atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag
    50161 aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg
    50221 agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag
    50281 atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca
    50341 aagtaggaag cggtacggca acagattggg atttggataa agatttaggt actacttatt
    50401 gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag
    50461 gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt
    50521 tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag
    50581 tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt
    50641 ccactgcatg actacccagg aattagatga actggctcat tcagaagcag gagcatttat
    50701 cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt
    50761 tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga
    50821 gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat
    50881 tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt
    50941 taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc
    51001 ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc
    51061 gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat
    51121 cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta
    51181 tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg
    51241 cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa
    51301 accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca
    51361 tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga
    51421 aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc
    51481 aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga
    51541 ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc
    51601 attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat
    51661 gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt
    51721 acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt
    51781 gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg
    51841 gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact
    51901 tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa
    51961 gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa
    52021 aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca
    52081 ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct
    52141 ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg
    52201 ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga
    52261 gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg
    52321 atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat
    52381 ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt
    52441 agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat
    52501 gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag
    52561 atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct
    52621 gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc
    52681 tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa
    52741 aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa
    52801 atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt
    52861 tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca
    52921 ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg
    52981 acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg
    53041 ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg
    53101 aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta
    53161 gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct
    53221 ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg
    53281 catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg
    53341 tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca
    53401 cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa
    53461 gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac
    53521 gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg
    53581 gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat
    53641 atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta
    53701 ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa
    53761 aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa
    53821 atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga
    53881 caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga
    53941 tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac
    54001 ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt
    54061 tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc
    54121 tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct
    54181 gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat
    54241 ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg
    54301 cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt
    54361 gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc
    54421 tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca
    54481 aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc
    54541 actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg
    54601 ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct
    54661 attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa
    54721 tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc
    54781 tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct
    54841 gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa
    54901 tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca
    54961 tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact
    55021 gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg
    55081 cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg
    55141 gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag
    55201 caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga
    55261 cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa
    55321 atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat
    55381 gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa
    55441 atatgtacta tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac
    55501 tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg
    55561 ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc
    55621 aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca
    55681 aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac
    55741 tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg
    55801 tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact agtcgctttc
    55861 gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg
    55921 ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg
    55981 cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag
    56041 ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac
    56101 gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc
    56161 ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta
    56221 cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag
    56281 tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc
    56341 caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat
    56401 tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca
    56461 aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct
    56521 tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac
    56581 gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct
    56641 gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca
    56701 actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa
    56761 ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat
    56821 atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga
    56881 ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa
    56941 ataatcatca attgactata aaatcatctg agcaaagata aagacattaa cactactgaa
    57001 aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag
    57061 aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata
    57121 gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc
    57181 ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc
    57241 tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa
    57301 acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga
    57361 tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat
    57421 aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac
    57481 ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt
    57541 atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt
    57601 attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt
    57661 cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc
    57721 agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat
    57781 tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt
    57841 ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg
    57901 ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct
    57961 tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag
    58021 atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag
    58081 caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt
    58141 tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga
    58201 aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac
    58261 cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca
    58321 acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt
    58381 ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa
    58441 gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag
    58501 aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta
    58561 attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat
    58621 tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca
    58681 taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa
    58741 ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa
    58801 caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag
    58861 aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa
    58921 cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag
    58981 caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc
    59041 tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa
    59101 ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa
    59161 aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga
    59221 ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga
    59281 ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt
    59341 tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt
    59401 ttatcataag caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag
    59461 acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt
    59521 attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt
    59581 tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta
    59641 atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt
    59701 tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga
    59761 cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat
    59821 ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct
    59881 tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta
    59941 ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt gattacagga
    60001 aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag
    60061 gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc
    60121 ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta accagctgca
    60181 tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct
    60241 gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca
    60301 aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt
    60361 ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt
    60421 ccactatagc gagtgataac gacactgttg attaataagc cataaccacg taattcatcg
    60481 attaaccgca agatatccat atcataagta atcccataat caccacggat cttattacgt
    60541 tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc
    60601 aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca
    60661 actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata
    60721 tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc
    60781 cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga
    60841 aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg
    60901 gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc
    60961 atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga
    61021 attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca
    61081 tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc
    61141 atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac
    61201 caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt
    61261 acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa
    61321 gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga
    61381 atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa
    61441 gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag
    61501 aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa
    61561 gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt
    61621 tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca
    61681 aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa
    61741 tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg
    61801 actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg
    61861 aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata
    61921 aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc
    61981 aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc
    62041 agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg
    62101 tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag
    62161 caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg
    62221 aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa
    62281 acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat
    62341 gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact
    62401 ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg
    62461 tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa
    62521 gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta
    62581 ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca
    62641 agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa
    62701 cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg
    62761 aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa
    62821 gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat
    62881 gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag
    62941 atcacgatat tcaatcacaa attgaatcaa ttgcccatgc aagaacactt ggttttaccg
    63001 ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg
    63061 aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg
    63121 atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa
    63181 cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac
    63241 gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt
    63301 cctgattgga taagttggat taccagatgt cattcagata attctgttcc aaaggacttg
    63361 cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat
    63421 aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta
    63481 gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc
    63541 agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa
    63601 ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca
    63661 tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa
    63721 aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt
    63781 acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct
    63841 attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc
    63901 ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa
    63961 ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca
    64021 acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca
    64081 atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtgttctt caaaaaaggc
    64141 gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc
    64201 acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg
    64261 gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc
    64321 attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca
    64381 taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc
    64441 ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt
    64501 gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt
    64561 gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc
    64621 gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg
    64681 atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat
    64741 ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta
    64801 atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa
    64861 ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg
    64921 gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat
    64981 gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg
    65041 ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca
    65101 gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc
    65161 gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc
    65221 gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt
    65281 cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat
    65341 tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct
    65401 tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta
    65461 tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa
    65521 gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga
    65581 gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc
    65641 ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca
    65701 acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt
    65761 gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat
    65821 gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga
    65881 aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt
    65941 aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc
    66001 atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta
    66061 ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt
    66121 gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa
    66181 aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg
    66241 gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt
    66301 catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc
    66361 atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt
    66421 taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc
    66481 aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac
    66541 aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca
    66601 gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt
    66661 ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc
    66721 ttttttattt taggaattag agagggattt gtccttctat gattttttgc tataataggg
    66781 aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca
    66841 aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt
    66901 gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta
    66961 taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa
    67021 tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct
    67081 gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat
    67141 gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat
    67201 actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag
    67261 aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca
    67321 ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg
    67381 aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc
    67441 gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt
    67501 gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga
    67561 aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt
    67621 aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt
    67681 cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt
    67741 aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat
    67801 acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc
    67861 attgactgga atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc
    67921 tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac
    67981 gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt
    68041 agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata
    68101 cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt
    68161 tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac
    68221 tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca
    68281 gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat
    68341 tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa
    68401 tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg
    68461 aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat
    68521 atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat
    68581 taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa
    68641 gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa
    68701 cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt
    68761 tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga
    68821 gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa
    68881 cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa
    68941 gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg
    69001 tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata cctacgctta
    69061 tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc
    69121 gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt
    69181 tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg
    69241 tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt
    69301 agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga
    69361 aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc
    69421 catttcagcg caataattcc ttttattcaa aaaacatacc aaaaatattc caacatcatc
    69481 ttggactttc tacatgttac taatgagcgt gtaaaagaac gagatggtgt ttttatttct
    69541 atcaatgaag ctctttttct ttgcaatcct cacttcaatc ctctataatg tcgcatatag
    69601 tttagtaaat gagtaaacta ctaatttact tatttactaa aaataaaagg atgaggagtt
    69661 aaaataatgg aaataccaac aaatttaaaa cataaaccag taatcgttgt agaagactat
    69721 gataaagttg atggaagaaa tgcattaaat acggatgcaa aagggctttc actgggcctt
    69781 gctcagtgga atgatcgtgg aaaagtcgat atctcaggca aggtatggcg acatactgga
    69841 gaaaaatggt cacgacagtc ggaagaatta ccaatcacac gtattttaga tttagcgatc
    69901 ttagctgcac aaggaagttt atacttccaa gatgcttatc gttatgaaaa attttatgat
    69961 ccagaaaatc cagtcgtaga catcattgga ttacaaggga accgaatgac ggtagaagta
    70021 gataccaaaa atccaaagat cgatgaagac atcattttgt attacgatac actacaaaaa
    70081 gacggagaac tgattgggga acgattccgt attttaaaac gtcttttaga tgagctagga
    70141 tattaataga cagagatgat caaaagggga agagaagcat ggagaatttc tcactaacag
    70201 aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg gaagaaaccg aaaagccatc
    70261 ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa agggaattgg caagatcctg
    70321 aaatgaaaat cagtaacgta cttttggaag gagatgccgg gtcggggaag acacaattag
    70381 ccaaagcact atctgctgat ctgcaacttc cctatacaaa agtgacctgt tttgcagata
    70441 tggacaaatc agatgttttc ggagccttat tacctgtagt agattctaat aaggaacaag
    70501 accaagaatt actagaagct atttaccaaa cagatacact tgaagctgta ctgacactga
    70561 tccagaacca ttacggcatt gatccttttt ctgctaaaga aaaattagca gagctagttc
    70621 aaaggatcga agctgattat ccagatgcaa tccattacag ctattaccct tctgaaatcg
    70681 ttcgagcgat tgaaaaagga tatttattag agattcaaga gccgactgtc atccgagatg
    70741 cctctgttct tgtggcactt aactcagcgc tcgaaccaaa tgggatgttg aatttaccga
    70801 caggaatcat tcgtcgccat ccagattgcg tcattgtgat cactacaaat cgaaattacc
    70861 aaggaaatcg cccattaaat gaatctttaa gagatcggat gcaacatgcg gaaaaaatgg
    70921 atcttcctgc cttagaagtc atggcagaac gtgcgatggc gaaaactatg atccaaaaac
    70981 aagaacttct cttaaagatg gcagagatta tccgtttgtt ggatgaaact gcaaaagcta
    71041 acgccatcaa aggtgtagca ggaatgcgtt cttattttta ttgggcaaat acctataagc
    71101 aaggacaaga tttactccag tcgatttatc caaaagtttt gtataaatta tccactgatt
    71161 cagatgaatt ggcaattctt gaacaagctt tgtctgacag tggattgcta ctggaacttc
    71221 aggacatatt gagaaaagaa aaatggggtc atcctcaatc tgagcataca aaaggaagaa
    71281 ccattagcgc cgaagaagca aatgaacgaa acatcactga aaatgctgaa gaaattctgc
    71341 gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa tgagggagaa gagaaacaag
    71401 aagaaacaat agaaaaaaac gagattcctc ctcaaatgca aaaagaacgc agtagcgata
    71461 tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga ttctcaagaa atgtctgctt
    71521 ctgacatgga agcacatgat aaggaaatca agaagcaact gaataaagaa gcgcgacaaa
    71581 tcatgaagga aacgattcat gaaaaagaag gtctcatcgt tcaccgacca gctttccaag
    71641 gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat gccaatagta gaatcactta
    71701 gccggcaaat tttagagtta ctggataatg aacaaacaga gacgtaccaa aaaggaaaat
    71761 acgaaggtca gcggttcaat gcaagcaaag tagcatatgg tgatttgaga aattttgaca
    71821 aaaagaaccc gccacatgag caaccttctt tagcggtagc tgtacggatc gatgaatctg
    71881 ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa agcagcgatt gccattgccg
    71941 aattttctaa gaaagtcgat atcccattgt tgatttacgg agatacagca gaccgttcgt
    72001 caagagaaaa gacttctttg ttttcttata aagaatttga agacggattt caatggttaa
    72061 atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg tgatggtgct gcacttcgtt
    72121 tagcagcaga aaaattaaac agacaatccg caacaaccaa actgttgctt aatattagtg
    72181 acggtcagcc aaaagcgttg ccggattata caggcaaaaa agcaaaagaa gatatccagt
    72241 ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt ctccgcagct attggccaag
    72301 acaaagaaga aatcaagtcg atctacggtg aaagtcgttt tgtagatatt actgacttga
    72361 atgaatttcc caagcaaatg attcaactga ttgcccgcta tctatgataa aataaagctc
    72421 aacgaacgca cttagcagat tcgttgagct tttcgtttct agttaccggt aacaaggata
    72481 cttctaaaat agaatcggtt ataatcaaga gggaccgtta aaggagtgaa acgatatgat
    72541 acaagaacaa aaatggtgga aagaagcagt aggctatcaa atctatcctg ctagtttcaa
    72601 agatagcaat ggcgatggtc aaggagacat caatgggatc cgtgaaaaac ttaattattt
    72661 gaaagatctt ggtatcggct ttatctggat caatccgatc tatcaatctc catttgtcga
    72721 taatggttat gacgtaagtg actatcaaga tatagaagag agattcggaa cgatggaaga
    72781 ttttgattta ttgctaaaag aagcacatga attagggatc aaagtgatta tggatcttgt
    72841 catcaaccat agttcggatc agcatcaatg gtttgaagaa tcgaaaaaat ctaaagaaag
    72901 cccgtatcgc gattattaca tttgggtcga cggggtagat ggcaaagaac cgaataattg
    72961 gacatcgatt ttcggcggtt ctgcttggga gtattctcat gaaactggac agtattatct
    73021 gcatgttttt gcaaaagaac aaccagactt aaattgggaa agcgagaaat tgaaagaaga
    73081 actattcaat atgatccgat ggtggttgga taaagggata gatggatttc gtttagatgc
    73141 gatttctcat gtaaaaaaag atgaatattc agtaaaagcc acagagaacc ctttctcacc
    73201 ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat ttgaaacatg tttttgaaga
    73261 gtacgatatc atgactgtcg gagaagcaag tggtgtcact gcagaagaag gaccacaatg
    73321 ggtagggaaa gacggttatt tcgatatgat attcgaattt gatcatatcc atatctggca
    73381 gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa cttgcattaa gcgcttggca
    73441 aacttcattg gatggtatcg gatggaacgc tttatatatg gaaaaccatg atgttcctcg
    73501 tgccgtttca gtgtttggag atactcgtcc cgatttttgg gctatgtctg caaaagcaat
    73561 cgctatgatg tatttctttt tgcaaggtac gccatttatt tatcaaggac aggaaatcgg
    73621 aatgacgaat atgccctttg aatcgatcga tcaagttgat gcagtagatt cgaaaagact
    73681 ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa gcattggata tcattcgtga
    73741 aacaacgaga gacaacagca ggacacccat gcaatggact tcggaacaat atgctggttt
    73801 ttctacacat gaaccttggc tgatcactaa tccaaatacc aaaacgatca atgtagaaca
    73861 acaagaatat gaaccagagt cggtcctgca attctataag aacatgatcc gcattagaca
    73921 aaccaataaa ggtctgatat acggttctta taaagaatat cttcatgaac atccacaact
    73981 ttatgtctat gaaagatatt tagaagatga acaatatctt attatggtaa acttaacaga
    74041 atcacttgct gactatgaac ttccaaaaga agcagatcag tcctggacat tactcttgtc
    74101 aaattcctct agcggagaat ttgaagctaa aggcatacta gcaccttatg aagctcggtt
    74161 atataaaaca aacaagtaaa acttcctcgt taaagttcat tagttggact ttgcgaggaa
    74221 gtttttcaga ttgctctgtc aattactcat ttcaata
//
LOCUS       DAFEZB010000004        68907 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFEZB010000004
VERSION     DAFEZB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.004"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(105..629)
                     /locus_tag="LP859_000316"
     CDS             complement(105..629)
                     /locus_tag="LP859_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3028299.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LP859_000317"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LP859_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL3028300.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LP859_000318"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LP859_000318"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL3028301.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="LP859_000319"
     CDS             complement(2351..3031)
                     /locus_tag="LP859_000319"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL3028302.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="LP859_000320"
     CDS             3157..4026
                     /locus_tag="LP859_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3028303.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="LP859_000321"
     CDS             4052..4567
                     /locus_tag="LP859_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028304.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="LP859_000322"
     CDS             complement(4614..5207)
                     /locus_tag="LP859_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3028305.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="LP859_000323"
     CDS             complement(5232..5411)
                     /locus_tag="LP859_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028306.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="LP859_000324"
     CDS             complement(5488..5997)
                     /locus_tag="LP859_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028307.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="LP859_000325"
     CDS             complement(6093..6473)
                     /locus_tag="LP859_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3028308.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6802..7551
                     /locus_tag="LP859_000326"
     CDS             6802..7551
                     /locus_tag="LP859_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL3028309.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7735..9012)
                     /locus_tag="LP859_000327"
     CDS             complement(7735..9012)
                     /locus_tag="LP859_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028310.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="LP859_000328"
     CDS             complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="LP859_000328"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL3028311.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9934..10878)
                     /locus_tag="LP859_000329"
     CDS             complement(9934..10878)
                     /locus_tag="LP859_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL3028312.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10880..11566)
                     /locus_tag="LP859_000330"
     CDS             complement(10880..11566)
                     /locus_tag="LP859_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL3028313.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12040..12360
                     /locus_tag="LP859_000331"
     CDS             12040..12360
                     /locus_tag="LP859_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL3028314.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12514..13419
                     /locus_tag="LP859_000332"
     CDS             12514..13419
                     /locus_tag="LP859_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028315.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13416..14189
                     /locus_tag="LP859_000333"
     CDS             13416..14189
                     /locus_tag="LP859_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3028316.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14370..15794)
                     /locus_tag="LP859_000334"
     CDS             complement(14370..15794)
                     /locus_tag="LP859_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3028317.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16005..16451
                     /gene="nrdI"
                     /locus_tag="LP859_000335"
     CDS             16005..16451
                     /gene="nrdI"
                     /locus_tag="LP859_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL3028318.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16516..18498)
                     /locus_tag="LP859_000336"
     CDS             complement(16516..18498)
                     /locus_tag="LP859_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3028319.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="LP859_000337"
     CDS             complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="LP859_000337"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL3028320.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19411..20472)
                     /locus_tag="LP859_000338"
     CDS             complement(19411..20472)
                     /locus_tag="LP859_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL3028321.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20683..21249)
                     /locus_tag="LP859_000339"
     CDS             complement(20683..21249)
                     /locus_tag="LP859_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3028322.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21277..22779)
                     /gene="eat(A)"
                     /locus_tag="LP859_000340"
     CDS             complement(21277..22779)
                     /gene="eat(A)"
                     /locus_tag="LP859_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL3028323.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23037..24158)
                     /locus_tag="LP859_000341"
     CDS             complement(23037..24158)
                     /locus_tag="LP859_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL3028324.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24284..25225
                     /locus_tag="LP859_000342"
     CDS             24284..25225
                     /locus_tag="LP859_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL3028325.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25375..26271)
                     /locus_tag="LP859_000343"
     CDS             complement(25375..26271)
                     /locus_tag="LP859_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL3028326.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26540..27880
                     /locus_tag="LP859_000344"
     CDS             26540..27880
                     /locus_tag="LP859_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3028327.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28098..28541
                     /locus_tag="LP859_000345"
     CDS             28098..28541
                     /locus_tag="LP859_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL3028328.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="LP859_000346"
     CDS             complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="LP859_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL3028329.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30866..32089)
                     /locus_tag="LP859_000347"
     CDS             complement(30866..32089)
                     /locus_tag="LP859_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL3028330.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32120..32959)
                     /locus_tag="LP859_000348"
     CDS             complement(32120..32959)
                     /locus_tag="LP859_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL3028331.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32976..33656)
                     /locus_tag="LP859_000349"
     CDS             complement(32976..33656)
                     /locus_tag="LP859_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028332.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33658..34731)
                     /locus_tag="LP859_000350"
     CDS             complement(33658..34731)
                     /locus_tag="LP859_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028333.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34787..35025)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35068..35301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35378..36049)
                     /locus_tag="LP859_000351"
     CDS             complement(35378..36049)
                     /locus_tag="LP859_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3028334.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="LP859_000352"
     CDS             complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="LP859_000352"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL3028335.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39473..39910
                     /gene="ndk"
                     /locus_tag="LP859_000353"
     CDS             39473..39910
                     /gene="ndk"
                     /locus_tag="LP859_000353"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL3028336.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39932..40798)
                     /locus_tag="LP859_000354"
     CDS             complement(39932..40798)
                     /locus_tag="LP859_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3028337.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40958..43051)
                     /locus_tag="LP859_000355"
     CDS             complement(40958..43051)
                     /locus_tag="LP859_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL3028338.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43800..44423)
                     /locus_tag="LP859_000356"
     CDS             complement(43800..44423)
                     /locus_tag="LP859_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028339.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44582..45979)
                     /locus_tag="LP859_000357"
     CDS             complement(44582..45979)
                     /locus_tag="LP859_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3028340.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46011..46982)
                     /locus_tag="LP859_000358"
     CDS             complement(46011..46982)
                     /locus_tag="LP859_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3028341.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47356..47781
                     /locus_tag="LP859_000359"
     CDS             47356..47781
                     /locus_tag="LP859_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028342.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48269..48970)
                     /locus_tag="LP859_000360"
     CDS             complement(48269..48970)
                     /locus_tag="LP859_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL3028343.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48986..49426)
                     /locus_tag="LP859_000361"
     CDS             complement(48986..49426)
                     /locus_tag="LP859_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL3028344.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49426..50001)
                     /locus_tag="LP859_000362"
     CDS             complement(49426..50001)
                     /locus_tag="LP859_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL3028345.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50849..51328)
                     /locus_tag="LP859_000363"
                     /pseudo
     CDS             complement(50849..51328)
                     /locus_tag="LP859_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51487..52188)
                     /locus_tag="LP859_000364"
     CDS             complement(51487..52188)
                     /locus_tag="LP859_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3028346.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52296..53705)
                     /locus_tag="LP859_000365"
     CDS             complement(52296..53705)
                     /locus_tag="LP859_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL3028347.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53753..55618)
                     /locus_tag="LP859_000366"
     CDS             complement(53753..55618)
                     /locus_tag="LP859_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3028348.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55739..56590)
                     /locus_tag="LP859_000367"
     CDS             complement(55739..56590)
                     /locus_tag="LP859_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3028349.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56900..57613)
                     /locus_tag="LP859_000368"
     CDS             complement(56900..57613)
                     /locus_tag="LP859_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL3028350.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57681..58007)
                     /locus_tag="LP859_000369"
     CDS             complement(57681..58007)
                     /locus_tag="LP859_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL3028351.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58130..58357)
                     /locus_tag="LP859_000370"
     CDS             complement(58130..58357)
                     /locus_tag="LP859_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL3028352.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58679..59932)
                     /locus_tag="LP859_000371"
     CDS             complement(58679..59932)
                     /locus_tag="LP859_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL3028353.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59993..60733
                     /locus_tag="LP859_000372"
     CDS             59993..60733
                     /locus_tag="LP859_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3028354.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60819..61577)
                     /locus_tag="LP859_000373"
     CDS             complement(60819..61577)
                     /locus_tag="LP859_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL3028355.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61574..62602)
                     /locus_tag="LP859_000374"
                     /pseudo
     CDS             complement(61574..62602)
                     /locus_tag="LP859_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62622..65789)
                     /locus_tag="LP859_000375"
     CDS             complement(62622..65789)
                     /locus_tag="LP859_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028356.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66013..66897)
                     /locus_tag="LP859_000376"
     CDS             complement(66013..66897)
                     /locus_tag="LP859_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL3028357.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66992..67429)
                     /locus_tag="LP859_000377"
     CDS             complement(66992..67429)
                     /locus_tag="LP859_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028358.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67646..68557)
                     /locus_tag="LP859_000378"
     CDS             complement(67646..68557)
                     /locus_tag="LP859_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL3028359.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        19756 a        14476 c        11868 g        22807 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aacagaaagt
     6601 aggtcctcca agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt
     6661 ttcaagaaaa caaacagaaa attatcataa cacaaattat ttataattga aagactagtt
     6721 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
     6781 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
     6841 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
     6901 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
     6961 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
     7021 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
     7081 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
     7141 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
     7201 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
     7261 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
     7321 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
     7381 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
     7441 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
     7501 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
     7561 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     7621 atcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
     7681 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag
     7741 taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg
     7801 ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt
     7861 gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc
     7921 ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa
     7981 tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa
     8041 accatagcga caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg
     8101 gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt
     8161 tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata
     8221 tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac
     8281 atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt
     8341 tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc
     8401 cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat
     8461 atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg
     8521 ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc
     8581 cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag
     8641 gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac
     8701 gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt
     8761 tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata
     8821 atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg
     8881 ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga
     8941 aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg
     9001 ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac
     9061 gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat
     9121 atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc
     9181 taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac
     9241 gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata
     9301 catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg
     9361 atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc
     9421 gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc
     9481 tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac
     9541 acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt
     9601 ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat
     9661 tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt
     9721 tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta
     9781 tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag
     9841 atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg
     9901 cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat
     9961 ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc
    10021 tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag
    10081 ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg
    10141 actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt
    10201 ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac
    10261 atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct
    10321 tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt
    10381 gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa
    10441 catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt
    10501 ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt
    10561 cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc
    10621 tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac
    10681 gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc
    10741 tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa
    10801 tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg
    10861 atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct
    10921 tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt
    10981 ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga
    11041 ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt
    11101 ccatagctga agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt
    11161 ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc
    11221 caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa
    11281 tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac
    11341 catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat
    11401 tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca
    11461 aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc
    11521 cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat
    11581 aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc
    11641 acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa aattatctta
    11701 cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga aataaggtct
    11761 agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa attgcaaagc
    11821 attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt tactaaaaag
    11881 tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata tacaatctgc
    11941 caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat gattttcctc
    12001 tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat ggaattaatc
    12061 atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga tggtttttct
    12121 aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta ctttttgtca
    12181 aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg gattggcgca
    12241 gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct ttccacttgg
    12301 ttctttgtgg ggatgctact catcagtatc attggcatca agattactag cggccactag
    12361 acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat tcaatttttt
    12421 tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat caggtgtatg
    12481 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
    12541 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atcccctggc
    12601 aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat gaaagctatt
    12661 ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga gatttctccc
    12721 tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg tatctatcca
    12781 ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt atctgctatc
    12841 gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc gaaatcctac
    12901 tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
    12961 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
    13021 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
    13081 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat catcaaacaa
    13141 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacaacaaat
    13201 gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga tcctgataaa
    13261 atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct attgtctgca
    13321 caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc ttctctattg
    13381 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta aaacaagaat
    13441 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgttttgc
    13501 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
    13561 ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca gctgcaagca
    13621 cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat cgaaagtatt
    13681 ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct gtttatttat
    13741 accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct tcgatttcct
    13801 tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact tatactttgg
    13861 gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct tgctttatca
    13921 atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat
    13981 ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat ccgatcagta
    14041 tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg actcatcttt
    14101 ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta gcattaggat
    14161 atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct gtgaaagaga
    14221 cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga aaaaggagct
    14281 gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac gacaggctca
    14341 caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt ggcaaataaa
    14401 accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc taagtggata
    14461 tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat gcctggcaag
    14521 aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc ccatacttga
    14581 aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg ataagaaagt
    14641 ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg
    14701 taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac attcgtacat
    14761 aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt tccccatgct
    14821 ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa gatcgtccat
    14881 gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac tgctccccat
    14941 ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat cgtttgcagc
    15001 tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat aataatcaag
    15061 ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc tcgtgaacct
    15121 acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat gcaactcaat
    15181 acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc gctagaaaag
    15241 ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa taaaaataat
    15301 ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc gactccccga
    15361 ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga tgccagattt
    15421 tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa
    15481 gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa accagcgaaa
    15541 cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg acgtttttta
    15601 ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag tacccaactg
    15661 aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa tacgccacct
    15721 ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc ttttttctgt
    15781 tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa aagtataaca
    15841 aacctttttt atttttactt aattaataaa aactttaata gaaatataat tctaccggct
    15901 tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca ccaaaaaact
    15961 taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac attttatata
    16021 tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa
    16081 tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat gaaaatagtg
    16141 attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta gacggcggaa
    16201 atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa tatctggaac
    16261 atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa aacttcaata
    16321 atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct ttcttggcag
    16381 attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt ttagcagaaa
    16441 acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc tttcgtttct
    16501 attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt ttgctttacc
    16561 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
    16621 tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa gcgtatcagg
    16681 tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta agtcaactgt
    16741 gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc caatagcgtg
    16801 tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat gtccgttttc
    16861 tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat cacttgcttt
    16921 atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac tttcagctga
    16981 agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag tcgctaattc
    17041 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
    17101 ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga tcaatgggat
    17161 caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca ttccgatcac
    17221 tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta gaggtaatgt
    17281 tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag ctcctttgat
    17341 catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg ctgcacccac
    17401 ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag tttcatcaat
    17461 catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca tcggcaagaa
    17521 taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc agttgatccc
    17581 accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag
    17641 accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat caggtactac
    17701 tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga cacctaggat
    17761 cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg gtgcttccgg
    17821 attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg gtgttgcacc
    17881 aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca tcccgttttt
    17941 caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg ttccggctgt
    18001 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga caaatgcagg
    18061 aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt tagatggttt
    18121 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
    18181 agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc ctaatttcac
    18241 gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc cgatgatgac
    18301 ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta attcagagac
    18361 atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt ggacaatgct
    18421 ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt cttccaaagt
    18481 cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca aatccgtttg
    18541 cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc atgacgatag
    18601 ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg taatcagctt
    18661 ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt gttggtttga
    18721 catcgaccat caagttattg aatactttac ctataccaat catactgata gtagaaatca
    18781 tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
    18841 ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg gcttgtttat
    18901 tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat ccaccgatca
    18961 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gtcaaattca
    19021 gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg acagctacag
    19081 tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct acaccgaatg
    19141 tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg gctcccatat
    19201 aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact ggttcgatac
    19261 ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc attagttgaa
    19321 cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg cgttccgttg
    19381 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
    19441 gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa ctttagctac
    19501 tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct cacccatata
    19561 gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag ctgtatttct
    19621 tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga catctcttgc
    19681 atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt catctacagg
    19741 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacgtt
    19801 ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta atagatctag
    19861 agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc cttgatatag
    19921 tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac cttgaatttt
    19981 gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg gacgtggata
    20041 ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca attcatcgat
    20101 atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg accagtcggc
    20161 aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa ttgctttcaa
    20221 aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg aaatatcgac
    20281 catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gcttttgata
    20341 tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
    20401 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca taaatattga
    20461 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
    20521 atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc atttctaaag
    20581 gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc taagcaaaag
    20641 cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat aagaaaccaa
    20701 ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat gaaaataagc
    20761 attgatcact cttatcaagg cattatggca aactaacaga tagacatttt cttcgtcttc
    20821 gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg actccccatt
    20881 tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag
    20941 tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca gcggcaagct
    21001 gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg aatggatgat
    21061 tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg cctgcatctg
    21121 tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg cgttccaagt
    21181 tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg caaagttccc
    21241 cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa gagaaataat
    21301 ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca tggcaggttt
    21361 cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat aattcaatgg
    21421 ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt ccactttttt
    21481 ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt ccatcccaag
    21541 ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg caaattccgc
    21601 caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca gatgttttgg
    21661 ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat ggataatgga
    21721 tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct cgattgtaaa
    21781 atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta cgcttaaaag
    21841 tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt cgatatcttt
    21901 taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg ctttggatcg
    21961 tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc tagattcagt
    22021 atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag accattcggc
    22081 tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt cattttgagc
    22141 catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat tcccttgata
    22201 aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat cgataaatcc
    22261 ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag ccgctacttg
    22321 ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga aatgagtgtc
    22381 atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata gtgtgctgaa
    22441 ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt cgatttccca
    22501 tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt tatcttttgt
    22561 ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat aaggtagttt
    22621 gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga taagtcctag
    22681 tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc cttggctgtc
    22741 gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa
    22801 agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata cacacaaaaa
    22861 gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct tccttgtctc
    22921 cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa tgcacggatc
    22981 cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt tgtcaactac
    23041 ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt tttttctaat
    23101 gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat acttaagaca
    23161 attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca aatagcgatt
    23221 attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt tccgtggatc
    23281 gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc gatctttttt
    23341 tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa
    23401 aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc taaatccacc
    23461 tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa gaaagctagc
    23521 gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg ttgacctgta
    23581 tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag aacgtttctt
    23641 cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta cccttctaaa
    23701 gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct tcctcctaaa
    23761 gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc tcctaaggca
    23821 taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc taaccccaat
    23881 gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa taaatagctg
    23941 atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt tcccctgagc
    24001 gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac attgattttt
    24061 cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa tgaagaagca
    24121 cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca ctttcttctt
    24181 aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct tccatttttc
    24241 tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa tcgcaatagc
    24301 tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg gaaatgaggt
    24361 cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg ggttgattgc
    24421 tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag aagaaatcat
    24481 cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc aattagatga
    24541 tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt gtttactaaa
    24601 tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata tcttatttgg
    24661 gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt taggtgatgg
    24721 agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa aagttgtaga
    24781 agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt attccatttg
    24841 gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg attgtaatat
    24901 tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg tatctgaatt
    24961 tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt accaacatat
    25021 tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt accaagacct
    25081 gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt ggcgaaaagg
    25141 acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag tccatgcaaa
    25201 agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg aatcagcagc
    25261 tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc tcatgatgcc
    25321 ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg ttttttattt
    25381 gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat cgttagtgat
    25441 aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata ggattggctt
    25501 aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat
    25561 attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca ctagtttctt
    25621 ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct cccgcaacag
    25681 ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag taagatatcc
    25741 aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat ctctttttat
    25801 aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg caccaaaaaa
    25861 tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa atgaactatt
    25921 atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct catcgattgc
    25981 cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa
    26041 aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct ccatatcaaa
    26101 tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag ctgatttaat
    26161 gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga tggaaggcaa
    26221 aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca tcttttccgc
    26281 ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa agccagtaaa
    26341 acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg aaataaatca
    26401 ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt
    26461 tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg attgtgaatt
    26521 taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta gctccagaca
    26581 tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag gaagtggcga
    26641 agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg ggctgatttt
    26701 atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg
    26761 attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta ctgattgggt
    26821 ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg cttccttttt
    26881 gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg ctgttttaac
    26941 tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt ggctggcttt
    27001 tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg tcggtcagca
    27061 aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg gtgggtttac
    27121 tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt cttacggcgg
    27181 ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg cgattcctaa
    27241 agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt tgtttttgct
    27301 tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt ttgtgatggt
    27361 atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga ttgtccttgc
    27421 tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt tcttccgttt
    27481 gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc aagtaccgca
    27541 acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt cttattttgt
    27601 cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat tattgatctg
    27661 gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat tatgggataa
    27721 aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta tcctgttcac
    27781 gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt atttcagtta
    27841 tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga aaattttatt
    27901 ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa gtcttgttgc
    27961 aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt caaaaaccct
    28021 ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa
    28081 aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa
    28141 cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat atcttcttca
    28201 gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc atcatgctgt
    28261 tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta gccgctagtc
    28321 ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc aacgacacag
    28381 cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt aaacgagctg
    28441 atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca caagaagaag
    28501 agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc gtccaaataa
    28561 aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct acttctggaa
    28621 cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    28681 aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    28741 aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta gagagacaaa
    28801 tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt
    28861 gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt aagaaaaatt
    28921 ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga aactggcttt
    28981 tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat ccctttttat
    29041 gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc acaaattttt
    29101 ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa agcaaactga
    29161 aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct acgaaacttc
    29221 cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct agcgttctac
    29281 ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca ttaccaggca
    29341 gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt gtcatcaatg
    29401 ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc atcacggttg
    29461 agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata atcaatgaca
    29521 cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt tgttggtttc
    29581 cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag gaagtttgtg
    29641 ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc gttaaaattg
    29701 ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt gaccagattg
    29761 aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa agacttgcgg
    29821 ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat gccgggacag
    29881 ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata gctgctgcta
    29941 gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct gaaacaatag
    30001 ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa aatgcaatcc
    30061 caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt gcacacacaa
    30121 caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg accattagag
    30181 ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa ataaaaataa
    30241 agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg
    30301 ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag atcgtgatat
    30361 taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata accattaata
    30421 caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc tctccttttt
    30481 tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc aattcatccg
    30541 cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa tgactccgcg
    30601 gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt caaatgtatg
    30661 gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac cgactttctg
    30721 aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt accttccata
    30781 agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt atttttataa
    30841 ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt tttcttgcta
    30901 ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc aacagatcaa
    30961 gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt
    31021 tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag attgctaccg
    31081 taggctggat tgttggtatg acttggctga catttcctac atcagaagaa ccgagacttg
    31141 ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca tgctggaaga
    31201 ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg aacaatccaa
    31261 actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc actttttcat
    31321 atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat
    31381 ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata ttgacatctt
    31441 ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat gcttccaaag
    31501 catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa aatttgatgt
    31561 cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc
    31621 ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc
    31681 cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat
    31741 caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac aaattatgcc
    31801 cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggac
    31861 cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt
    31921 ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat
    31981 agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac
    32041 gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct
    32101 cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc
    32161 cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt
    32221 tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc acataaggac
    32281 gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat
    32341 ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt
    32401 gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag
    32461 tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa
    32521 gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat
    32581 cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga
    32641 tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga
    32701 aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg taatctgtaa
    32761 atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata
    32821 catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg tcagttgtat
    32881 ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa
    32941 aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt
    33001 tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc
    33061 gttacatctg tttggaatcg attataccct ctcgatatag caagattccc taatccgcct
    33121 gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag
    33181 acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat accgattgga
    33241 ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc
    33301 tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc
    33361 gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata
    33421 atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg
    33481 ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat gattccaatc
    33541 atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca
    33601 aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta
    33661 tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta aaatatttat
    33721 ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg tttctccagc
    33781 taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa
    33841 aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg
    33901 atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga
    33961 atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt
    34021 ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt caacgattct
    34081 tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt
    34141 gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg
    34201 aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc
    34261 attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg
    34321 ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg
    34381 acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt
    34441 tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg
    34501 caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt ctcctgctgt
    34561 tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc
    34621 gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat
    34681 ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc
    34741 tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt
    34801 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga
    34861 tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga
    34921 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
    34981 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaag
    35041 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt
    35101 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
    35161 cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
    35221 gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac
    35281 tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactga
    35341 aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt tttttattta
    35401 acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat ttttcctttt
    35461 cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg
    35521 ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt
    35581 ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca
    35641 cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga tccaagatat
    35701 ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta
    35761 agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt
    35821 gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg
    35881 ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
    35941 cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt
    36001 tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
    36061 tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac agaagcaaag
    36121 aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt tcttcacagc
    36181 tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg
    36241 aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat
    36301 aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga taaatggtac
    36361 tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca
    36421 tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact
    36481 tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa
    36541 tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg cattccattt
    36601 ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac
    36661 atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa atggcaggaa
    36721 cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat
    36781 atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt
    36841 caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc
    36901 tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa
    36961 aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg
    37021 attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat
    37081 ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac
    37141 tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt
    37201 cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag caagataacc
    37261 aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt
    37321 cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag
    37381 tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt
    37441 agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg
    37501 atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt
    37561 ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc
    37621 taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg
    37681 atcatcttca tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa
    37741 tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga tggcattcaa
    37801 atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt
    37861 aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa
    37921 agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa
    37981 tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg
    38041 aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga
    38101 aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga caagattttc
    38161 gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata
    38221 gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat
    38281 atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc
    38341 cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga
    38401 tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct
    38461 gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc
    38521 taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg
    38581 cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat atgaaatcgc
    38641 attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga
    38701 tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt
    38761 ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc
    38821 tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg
    38881 agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg aatgactgtc
    38941 atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt
    39001 cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat ggggatttgc
    39061 ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa
    39121 aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg
    39181 cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct
    39241 ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt
    39301 cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt
    39361 tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct
    39421 cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag
    39481 aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcatcca
    39541 acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga
    39601 attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga
    39661 ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat
    39721 cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta cgatccgagg
    39781 agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc
    39841 ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc
    39901 ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca
    39961 gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga
    40021 attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat ctttgataat
    40081 aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgat aagctccgtt
    40141 tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag
    40201 tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc
    40261 gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag
    40321 gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt
    40381 cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt
    40441 tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc
    40501 tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca
    40561 ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt
    40621 ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt
    40681 tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct tccgctaatt
    40741 gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa
    40801 tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa
    40861 aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt
    40921 agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt
    40981 caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa
    41041 agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat
    41101 ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca
    41161 agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat
    41221 ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat
    41281 tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa
    41341 aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt
    41401 tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt
    41461 catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca
    41521 tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat
    41581 ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt
    41641 ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat
    41701 gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga
    41761 aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa
    41821 acggctgttg tagatgctca agatattggg cagattctct gaataaatct ttatctaata
    41881 tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat
    41941 aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa
    42001 cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt
    42061 ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca
    42121 tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga
    42181 tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct
    42241 tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt
    42301 tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa
    42361 cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga
    42421 ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa
    42481 atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa
    42541 taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga
    42601 taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc
    42661 agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca
    42721 ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa
    42781 taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg
    42841 ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg
    42901 ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat
    42961 agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac
    43021 gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt
    43081 tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt
    43141 tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc
    43201 gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa
    43261 agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc
    43321 cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc
    43381 caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg
    43441 ttccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    43501 agctgaccct cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg
    43561 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat
    43621 tttttgaaat tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca
    43681 cggtgttcaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    43741 ttttgccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctctgttttt
    43801 cattgtccat aggattgata ttgtccttgt gatgactcat tcgtaataat tgaggcttga
    43861 ttttcttcac ttcccgttat cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc
    43921 gactcgtttt tttgagaaga gacagttgtt ttcttgactt gttcatttgt ctttttccat
    43981 tccgctaaac gatcggcgac cacttttttg tctacttttt cagaataagc ggtgatcact
    44041 tttccatttt tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa
    44101 tgcttgtctt taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta
    44161 ataaattgat tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa
    44221 ttggcagtta ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct
    44281 actgattctt tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt
    44341 tttcctgaaa atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga
    44401 agaaacggtc tttttttcct catgctttcc tccactccta tttaattttg actattctat
    44461 tatatacaaa aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac
    44521 ttctttttca caaaatggta agaatggaga atttcacgca tttgctctca actattcttt
    44581 cttaagcctc aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga
    44641 ttgttctttt tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg
    44701 cattggtcca cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac
    44761 cttcttgaat ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat
    44821 catcgtggat catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt
    44881 tttcagaaat aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat
    44941 agattccttg ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt
    45001 cataattgtc gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt
    45061 ttgcttttac ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt
    45121 tgataaccgt caaatcctct gcatggatca caggcatgag atcaagttct tttactatct
    45181 caatcaattc acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga
    45241 acgcatctgc tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc
    45301 ttggtgtcag attcaagatg ataccaataa caccaccttg ctccatttca tggtaagatt
    45361 tgatggctaa cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat
    45421 ctgactcttc tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt
    45481 cattatgagt aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat
    45541 atttttcata ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca
    45601 tcggcatatc gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat
    45661 caaaataacg atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta
    45721 tcaaacggct ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact
    45781 gtacatcctc ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa
    45841 agaacttttg cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc
    45901 ctgaaatcct tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg
    45961 gaaattgata ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt
    46021 ccatgtatga tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt
    46081 cgttttaaca gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg
    46141 attttcggaa ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata
    46201 tccttcccga ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg
    46261 taattgctga caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag
    46321 ccgctttttc tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg
    46381 accgcttctt tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca
    46441 ataaatccga tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt
    46501 tctcgatcat tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg
    46561 ataactggaa tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag
    46621 aagacaatca gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt
    46681 tctggctgat cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt
    46741 tcgatccctg agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca
    46801 atagagaagc ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac
    46861 gctgaaatcg cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag
    46921 taacgggaaa ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc
    46981 atacattctc actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg
    47041 accgcattct ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta
    47101 gagcaacttc ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta
    47161 atatagcatt tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga
    47221 ttttttgcct ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt
    47281 ttttctcttc catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca
    47341 aatgggggaa gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg
    47401 acattgactg gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat
    47461 tcttctgtct taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta
    47521 cgtaaagata tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg
    47581 acacgcacgt atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg
    47641 ctgacagata tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat
    47701 aaaaaagaaa caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat
    47761 ggcaacatct acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt
    47821 tctgctatag taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc
    47881 tgattattgt acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag
    47941 cttgagcata tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc
    48001 ttatcctgtt caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca
    48061 ctatggctga tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt
    48121 aaaacaaaca gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa
    48181 atatttgaat ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt
    48241 ttctttcccg tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt
    48301 tatccgtaaa atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct
    48361 gacggatcac cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca
    48421 cttgtatttt aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg
    48481 tttcataata ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt
    48541 ttttgatacg ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc
    48601 gtttttttgg agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact
    48661 gctgtacttc gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat
    48721 cttcatagat ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta
    48781 aaaatcgctt gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat
    48841 aaaagagcgg caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct
    48901 cttttgcaag catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa
    48961 tcactgacat acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca
    49021 atgtttcaaa atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt
    49081 gtgtaatcat caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca
    49141 acatagttgc cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt
    49201 caaggatagc aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt
    49261 cctgcgccca tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt
    49321 agaaacttgt gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga
    49381 tttgctcctc tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt
    49441 caataataac tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt
    49501 gttggaaaag attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt
    49561 ttctaataat tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt
    49621 gtcgggcgtc agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc
    49681 tagacctcca gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc
    49741 atcgatcgtt tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa
    49801 aagcccattc ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat
    49861 cactcccata atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat
    49921 gtattttcct aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc
    49981 gcaaaaaaca gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct
    50041 acacgtgttt ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata
    50101 gacgccacta tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac
    50161 aaaaataaat aacgtgtttt catcgatatg aaatgactta cttatagaag atactgggat
    50221 ttcgtcctat atgccaataa cgtgtattat tttaaacaac aaatacttgt ttcttttatt
    50281 aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga taaatgatgt ttctctaaat
    50341 aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt ttgttcatca gcttctccta
    50401 aaaatctttt tgttaacgat aaatagctgg atttgtaaaa atatcacttg aatcgtcact
    50461 gctggaagaa aattcagtga taaccgcacc tgttgcgcct gcctggaact agtgtttgat
    50521 attgggctcg aacgtgtact gatctctagc atgaagaata atttcttttt ttactgtata
    50581 ataactttct ttcctttttg gaatagtaca ggaaagtttt tttgtttcct ttccttcgac
    50641 ataaggtata ccagaccctt tcgacaacga aaggttctca tttttccttc atcgtcattt
    50701 caaggagggt gccgatgctc tggacagttt cgatcgctcc taaaccaaaa tctgtatatt
    50761 cgattctttc taactctgat tctttcatac cattcctgtc tcaaggtaca tctattgtac
    50821 ttttttcttg taatccattc tgcctctcct aactaattgt caaatcatcc caagatatgt
    50881 cttcttcctc tttttcttct tggatgattt ctgcattcgg atctactggt tttttcaata
    50941 ataaaatggt tattcctgtg acaaaagaac cagctaacaa tgcaccaatg aatgcccatg
    51001 gtctggtcat cgtagcgact gcaaacattc ccgatgccgg aatacggcta tctgcgcccc
    51061 aaaacatgct taatccgcca cctactgtcc caccgactcc cgttgctatg acattgcgtg
    51121 ccaagtcgtt caatacgata gggaaacatc cctctgtgat ctgacagatc cccataggaa
    51181 aagcggtctt caatgtatcg atttcttgac gattgagtat atttttacga atgattttcc
    51241 ctaagaaata agcgatcgtc aagccgaatg gggttaccat cgaagctaaa atcaatacag
    51301 tgattggttc atagattcct tctgaaaatc gtgccacacc cccttatcca gaataaatgg
    51361 taatccagaa acagccgtgt atctgcggta ataagcctaa ccaaacgaaa aatatgagaa
    51421 gctatttttg tttggctgtt cttattactt gatgcagggc agctttttca caacctcttt
    51481 ttttatttat gtttcataat caatatggta aggatgcagc tccgtatggt agaccgcctc
    51541 cgaatattca atcatttccc caatttgcgt agacgatttc cgtatccgtt tgatgccgat
    51601 ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt aaaaatactg ccgagaaatc
    51661 atcagaaaat gagtcaatag tatatccttt tttcgtcagc aaacgataca aagattcttt
    51721 gccaaattct tctctgctga attgcatcat ttcttgtgga agataatgaa caaaataaat
    51781 ataaggctga ttgttcaaat aatataagcg cttaaaacaa acagcttgct caccgaaaaa
    51841 ggaaaacaaa ggatcttcct tctttatatg gaccaattgc aaatccagcg tttctttccg
    51901 gacttcatat cgggagttct ccaagatttg cgtaaagctt gccgctttgg ataatcgatt
    51961 gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt ttctttgcga catattcatc
    52021 tgatgccagc agttcgattg cctttcggac agtgattttg ctgacttgaa acagttcttc
    52081 taattcactt tctgtcggca acatcgaacc gactggatat tttcctgaga aaatatcttc
    52141 tttaatttta tctgcgactt ccaagtatag tacacgtgtc cttttcataa cgtcaccctt
    52201 tgctttacta ttactaaaag aatagcacat aaaaaataaa aaaagaacac aaatcacatg
    52261 tgagaagtgt gttctttttc attcagctgc tttaattaca agtttacgcc atttgaggca
    52321 attacttctt tataccaatc aaaagatttt ttctttgatc gattcagtgt cccatttcct
    52381 tcatcgtcta gatccacata gataaagccg taacgtttac tcatttctcc tgttgatgca
    52441 ctgacaagat cgatacatcc ccaaggcgta tagcccatca aatctacacc gtctgctact
    52501 gcattagcca tcgcttcgat atgacgacgt aaataatcga tccgataatc atcaataatc
    52561 gtgccatcct cttccatatg atcaatggct ccaagaccgt tttcaacaat gaataatggt
    52621 ttctggtaac gattgtataa ttcattcaat gcgattctca gcccttctgg atcgatctgc
    52681 cagccccatt cacttgcctc aagaaacgga ttttttactc ctcccagcaa gttcccttct
    52741 gaagttgctg cttgtggatc agtctcattg attgccgtag acatataata actaaatccg
    52801 atgtagtcca ctggatattc ttttagcaac tggaagtctt tttccgtttg ctctaatttt
    52861 tccggatcga ctccatattt tttatgggta cgcgctgtgt atgcagggta ttctccacgt
    52921 acttggacat ccgtgcagaa aaaattgaat tcttggtttt ggatcattgt tgctgcctga
    52981 ttgactggat tggcatcgat gctgtaagtt gttgcataga taatcatgca gccgatttgg
    53041 atatccgaac gcaattcgtg gccgattttt acggctttgc tacttgcaac gaattgatta
    53101 tgccatgctt ggaaaatatt ttggtactct cctgctccat tgctttttac taatccttga
    53161 cttaaagctg gaaagtgaaa ggccgaattg atttcattga aagtcatcca atatttgact
    53221 ttactgctgt atctctctaa tactgtctga gcaaatcgtt catagaagtc gatcagtttt
    53281 cgatttttcc agccaccgta ttcttttgcc aaatgaagag gcatttcgta atgagaaatc
    53341 gtgatcacag gttcaatatc atattttaag cactcatcaa tcagctgatc ataaaattct
    53401 aatcccgctt cgttaggagt accttcgtct ccatttggaa aaattcgtgt ccaggcaatc
    53461 gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg caagatcttc tttgaaacga
    53521 tcgtaatgat cgattcctcg atgattcggg taaatatatt tttcttgatc aatcgtccag
    53581 tcaaattctt cactgccgat aatcgcaaag cgctgcttac ctccaggcat agcatctgca
    53641 acggatagtc cttttccgtc tcgatcataa gccccttcta attgatttgg cagcagttgc
    53701 tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct ccttatttta
    53761 caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca agtatatcgg
    53821 caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca gctgctctaa
    53881 ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc ccttgtttga
    53941 ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata tgaatcaaaa
    54001 tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa atagtcgtga
    54061 tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg ataccttttc
    54121 ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt actttacccg
    54181 ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt
    54241 tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga
    54301 atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt gactctacac
    54361 catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg ctggagaaag
    54421 cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata caagcggcaa
    54481 tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg attccaaata
    54541 acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt gttcgcaatg
    54601 ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt ggcatcaata
    54661 catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc caatgcatgc
    54721 cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg attggactaa
    54781 atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc ccgattggtc
    54841 ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga actaaaatca
    54901 tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt tgaacccata
    54961 cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga atcactggga
    55021 tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct ggatacacaa
    55081 gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta gctgctgtaa
    55141 aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac aatatttgat
    55201 aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag aatcctttca
    55261 ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag ataatatcga
    55321 tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact gcaagacctt
    55381 cctcatccac gatcgattga taaacatctg ctacattact gccaatcact acttgatact
    55441 gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg
    55501 ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact aaacttttga
    55561 cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga ttatccattg
    55621 ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa aaacagtcac
    55681 aaacattcgt gaacctattt tttcaatcca ggttttgcct gccatacagt aacaatcctt
    55741 atgattggat gctttttttc tcacttacta ctctttgaat atgaatcgtt aaataaatct
    55801 gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag atactcattg atccgttcga
    55861 cacaatgaaa cgcttttgga tactttcctt gaactaatgc gtagagaaat tcatcttgct
    55921 cgtcagatcc ttcccctttg agataacgct gggcaaaaaa ctgcagatga gtaatgatac
    55981 gctgataatt caaagattct tcatcgaaca cgatgccgaa gaatttactg atgatactca
    56041 gtatatctct taccattgtg gtagcggtca tcgtcacttg catgtcttcg tttccttgtt
    56101 ggctattcac aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt tccacacccg
    56161 tcttttgttt cacgaacaaa agagcctctt tcgctgcttg atattctttc ggataaaatt
    56221 tttttgtttc aaataataat ggattaggca gttggatatt ttcttttgct cgtgtgatcg
    56281 cataatgaag atgatcggtc aatgtcagat aaatattagg cgaaaattca acttttagtt
    56341 tatcttcagc cagttcgact acttgtttga cccattcgat ctcctctgcc ggaatatcag
    56401 ccatcagctg ctggaattgg tttttagcaa aagaatctct taaaacaaat tctttttgga
    56461 ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct tttcccatac
    56521 aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc aaaattttat
    56581 caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat tcgcctactg
    56641 tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc ttattcaaaa
    56701 tcatcatacc atcgaatttt tatttatgca agccttttca tataaaaaat aaaaaaactg
    56761 gaacacaagt ctatctagaa aacaaccaaa tcagagatgc acctctttgc gtttctcttt
    56821 ttttcagcaa attggttttt ccttatcgat tcatatcctt tggctgtttt taatctgtaa
    56881 cgacttatgt cccagtatcc taaaatgttt ttgtctctag attgaaagca gatctcatat
    56941 catctgtcgc atcttttatc gatgtaacac tttccagttc tccttgtaca actagtcgcg
    57001 tgacggcgtc catgtcgcca caagtaatta gtgtgacaat ttcttttccc tcgacttcat
    57061 tcagtaattg ggtatctgta ggagcaactt tttctttaaa aaatgttttg tatgtataca
    57121 cattttctaa atctgtcaaa tagatcttgg cacccatctc taaattttct agcggtgtaa
    57181 acagcaattc agggttcgtt gcacgatggc ttgctaaggc ataatttcct tttcccattt
    57241 cttgatctgg agataatgtt cctgctccgt aaagtagtga cgtattatcc aagcctttga
    57301 aaaccggtaa attgattgaa actgaaggga ctgcgatact agcaatcact ggtagatctg
    57361 ttcctctcat ttgcgacctc atgacccctt ctgtcgtcat tggttctacc gcatcaaagt
    57421 caaaactgac atctttatcg ttattctttt tcagatcttc ttttgttaca ttcgctatag
    57481 catatttatc tcctgtctct ctgacaaaat aatccttgat ctgttcattg aaaatcaaag
    57541 ctagtcctac taataacaag aggaacaaga ggatattgac tagccatctt tttgcttttc
    57601 cttttttttt cattttctct ccctccagac tcattttact gtttttcttt tgctctttcc
    57661 aacagccgaa tgacacattt ctacaactct gtgtattttt ttcgtacacc tgcgcttttt
    57721 tctacaggat atttcaaatt atttttgatc aatttttctt caatgatctc atctagatcc
    57781 aggtgaaggt tgtctgccat catgtatgaa tagatcaata catctaccag ttcttctttg
    57841 attcgttcta attgtgtttc ttttacttct tccggcgttt tccattgaaa aagttctaac
    57901 aattcagatg cttccaaaga aatagagata gccaaatctt tttcgttatg aaattgtcgc
    57961 caattacgtt catctctaaa ctggttgact ttttccatac tattcatttg cttgccttct
    58021 tccaatcact tcttacttga ttaatcatac cattttttta cataaaaaat agatgatgct
    58081 tttaaaaaaa ctcctgtttt cttttattta aaataaatgt catagaaaat caataattta
    58141 ggtagccacc atgtgtatgc agtagcgtgt cttcttccct tgaggctttt tgtttttcga
    58201 tacgttcttc ttcccaaaca gacaacgcta ctagaatctc tcttaatgat tttccttctt
    58261 cggtgagcga gtactctact ttaggcggga cttggttata ggcttttcga tggatacttt
    58321 catcttgttc taactctctt aactgctggg tcagcatttt ttttgtgatc gtagggattt
    58381 gcttgcttct ccacgcctaa aataagcgat attgctcttc cctttatctg gtgtaagcaa
    58441 agcagttagc attttgacga tcgtgggttc cgcttttcac ttcttatcta cttagtatca
    58501 tgaagtatac tttcgtacta atactaagta gcggaagtgt caactacttt gtttaactac
    58561 atagcaaaag aaacagaagg gtgacaacgg tcatcacgct tctgtttctt ttgaatatgc
    58621 tgtgttgttg tctctatctc cgtgtagact ttcctcaaac ttatcctttg acagtaagtc
    58681 agtaggctga tttgctcttt ctagatgcca gatacatttc tacaaaaaaa tagtgattct
    58741 gttttgaaaa ttcgattttt agttctgcat tttcgtaaac atccaacatt tttttcgcac
    58801 tgtatagacc taacccgcca ttttttttag aggtttttcc tcttttgacc atctccgata
    58861 gcgtcaaatt cgttggattt gccttgttcg ctatactaaa acgtgatccc tctttttctt
    58921 ccagcagatt gatcgtaatc ggactctgat cggctacacg atgatcgaat gcgttagaaa
    58981 taaaaataga taaacagcga ataaaaacaa ccagatcaat cgggattgtt ttcacttcga
    59041 caggaataac tacttggatc gggattttct ccgattctgc tttttctaga aattttaaaa
    59101 agacacccct gacagctggg gatacaatat ttgttagctg ttgtatttgc ggtttttcaa
    59161 aaatagctgc agaatgatct tctaatattt ctaaaaattc caacgtttct tttttatttt
    59221 cctgaagagt aatttttaat gaaaacagta gacttttata atcatgtttg aattcctcta
    59281 gatgatggta atattcttca tttatttgat acagttgaag cgattcctga tgttgtcttt
    59341 cttttacttg gaaactgatc aacataatgg aaaaaaacaa agaaaaaaaa ctgatgacta
    59401 tcgacaaaag agatagaagc agataattag atgagcggaa gtgaacactt cccaatacca
    59461 agtaaatcac gccccagctt aataaaatac aaatacctcc aaaatagtgt tcagttggat
    59521 aagtaaaaag caagttgaga aatctcgtct gtttgtctat actttttact aatagataga
    59581 aaataaatgc gataagtagt gcaaaaaaac ttgctgttcc aagatgtctc tttcctaaat
    59641 aatccatcag atcaaaaacc agaaaaaagc tgagtccagt gatagcaaaa aaaagattac
    59701 tgatcaaaca gctgataaac aggtttttcg tagtaaaata aaaaagaaac atcaataata
    59761 agccaactag gtaaggaaag ctcactttag ggataggaag taaaagtgtc accaccatca
    59821 aagctataca accaaagctg ttttttacta gcagttgttt cgaaaagaat aaatagatac
    59881 ctaaatctaa aatcaaagta aaggtcaaat aaagtgtcac tgttgcgacc atcttattcc
    59941 tccattgttt tgtttcaaaa tgtacacata tttttttaat aggagcctgc atatgaaaaa
    60001 gtattttatc atagaagata accagtttca cagcgaatgg atcatcagag aattggtcaa
    60061 tcatcatatc gagaatatcg tgcagctcac ttctatctct caaatcaatc agttgatcca
    60121 gttgggcact attgataatc acgatgtttt ctttatcgat atcgacttga agcaatcact
    60181 cacaggtatt gatgtcgccg aacaaatacg taaaaaaaat gcacatagta atattgtttt
    60241 tttcaccgct tatacgaatt ttgcagtaga gatcatcaat cggaaaatcc ttccgattgg
    60301 atatatcaga aaagacagga atttatctga acaaattcgt caagtgctag aacagatcaa
    60361 ttggaaagaa cagcagatcg taaatgtcga taaaatcttt attgatcaaa aaggtggaag
    60421 tctcgtcctg tatcctcggg aaattttata tattgaaagt atcaaaggtt tgaaaaacca
    60481 agttatggtc aaaacaacta cagaagataa attagtgaat acttctttaa aacaattgaa
    60541 gacagagtta gaaaagtatg cttacttttt acttttaaaa tcttactgta tcaacttatc
    60601 gcaattgcag aaaattgatt ctgctcatta tgtgttagaa tttttcaacg gtgactcctt
    60661 actcgttggt cgaaagatct ttgaaaagac aaaggaacgt ttccatgact tccaaaaaac
    60721 tgcttcgagt tgatgaataa gagctgagac gtaagcatag cagatttcag ggagtgggcc
    60781 ttgaatcggc tccactccct gaaatcgttg atatgatttt acgcttcctc ttctgtagga
    60841 gcatctgtta acgaccatgt gaatgttgaa acgtagttgt ctacagcata attggataca
    60901 ggaacaaata attccactga ttgtctccct tgttccacat tctctcctaa tgaataaatc
    60961 catgtgccaa tcccttcatt tgctcctgca gttactagat ctactgcctg atctgctggg
    61021 atttcgattc cttcagcgcc agttccagaa acggtagatg gcattccact gctattagtt
    61081 gcgctatttc gaatcacggc gtttttgaat ttcacctttg aaccagttag tttattcgta
    61141 tcagatgttg taccaataaa ttctgttcgt ttgactgata atttccaacc ggcataattt
    61201 ccgcttttat ccgttacttg tacgaaattc tgtacttgtg tttgtgctcc agtactgtcc
    61261 gttccgcttt gtaatgcagc atatactgtt tttgctgtac ctaacttgat tgttccaaaa
    61321 tcaagatctg gagcttggtc aatcgtcagt aatccggatg ttccctgatt tggttcgtca
    61381 ggtgtaaaag gagtatcagg atcatttgga tcaacaggag gaactggaac aacgtcttca
    61441 ggatttgcag catctggttt ggtcacagaa atcgttcctt ctgtagtggc tgagctctta
    61501 ttctcagcaa aaacactttg tgtacataat aatagacttc cagctagaac ggtacttaat
    61561 gcaattgttt ttttcatttc tttttccttc tttcttttaa aataaataat ccagcaaata
    61621 agccaataag aatgactcca acaagtatca gccatcgcca gtcaaatgtt tctggctgat
    61681 tttttaaatc aatatccttt tcattcaatt ggtccgctgc atcatcagtt atttcaaaat
    61741 cggtatggag attccagcgt ttttctttcc aagcaccctt cacaaccgct tgataatgtc
    61801 ccggagctaa tttttttcct tctaatggga caagtaaatc aaaatttgta ttgggtgcta
    61861 catctatttt ttcttttttt attgaaaggt aaggctcttt tgtttctttt ttgtagattt
    61921 caaccgatgt agtcaagccg aacatataag aagcatttgg attttgaaga ttcgccttaa
    61981 tcacatttct tccatcgctt tgatccgcat aagctttttt taatttcaaa tctggggaag
    62041 actctttttc gctatttcaa atcaagattg ctttcacttg agaaaactga ttgacgacac
    62101 caactgtact tttgttagaa acgttctctt ttttttcttg aaacgtgatc cctcctgcga
    62161 ccacaccagc cagctctttt tctggcgctt tcagtgaaag tgtctcagtg accgcacttt
    62221 gaggaggaac agttatttct cccactgttt ttgctaagtt tttcatatca taacttaatg
    62281 tttcatcaaa tttacttttt gattctccat attcgatcac accgtttgaa ttcgtcgtag
    62341 cggagtggat caatgtctca actgttactg atttatctgt tcggttaaag accgtgtagg
    62401 ggatttcctg tgttttccca ggctccatcc ttacatttat atatgttttg ttcttatcaa
    62461 tctgattagc cggtaattca aaatctaccg caaaattgaa ttcggaggca tggacaggtg
    62521 tctgccaaag gatgaatcca aggagaagga tcataagtaa agagcagttg tttcgatgac
    62581 ttttattcca tattcgattc atcaatcatc ctccttgatc tttattctgt cggcgcatct
    62641 gctaatgtcc atgttatctc tgctgagtaa ttaccagcta tctgattttc tttggttaag
    62701 acaagtttaa acgcccgacc gttatttaaa tattctgaat aatctgcttg cacattcgtt
    62761 gaattttgtc ctctgacaat ttccagttct tcttcattca ctaattgata ctcctctcct
    62821 ttcttgaaat ataggacatc ctgcatagaa aatcctgcat catttgtgaa aggctgagat
    62881 aattttatcg ttaagatcca cggatttttc gtttctcgct gatcataaat ccataatttt
    62941 tcctgtatat ccatttggcc aaatgcttct tgtgtcaaac cagttgcttt gatctgacca
    63001 aaatcgagtt tggaaggggc aagaaatgag agaccacctt ctactacgat cttcgtagca
    63061 ggtgcaaaag gcggggcatc atgataaaca actggcgtat cacttggatt gtgattccca
    63121 atttcatcgt tggtacttgg cttgttctgc acacccttag cagtagcttc tcctgcttgg
    63181 tcgtttaata caatttctaa tacatctcct gcaaaaagtt gattttctgg gatagaaaga
    63241 acaaattccc ctgtatcatc aatagttgtt tctgacaaaa cagcttgatt ttttttcaat
    63301 gaaaccaagg ctcctgcttc acctgtacct gtgatcgtgc gagaagctgc attgatcaat
    63361 gtggtactta aattagcagg ttcaggagga atcacatcga ctgtcgtttt ggcagagtcc
    63421 agttctgtca cttcttggcc acctcttact tttttagcag caacaacttg cacttgatcc
    63481 tccgttttta atctctcatc attgggtact tgaacttcga acaagccgcc ttgttcgcct
    63541 tctccccatg ggttatatcc tgccccttca ttttcatctg tttgtccttg cgtgagagca
    63601 gtaatttcta cagatgattt cgtcgtatct gcgtaagtaa tacgtaaagt cacttctact
    63661 tcgcctgtcc aagcatctct tggctttgcc tcgacacctt cgggaacaac gacatgccca
    63721 tagatttttt tatctgcatt cgttggcact cttaaggatt caatcatcgg gagctgatta
    63781 ttggcactga ttcgattata atttttcatt ccggcacctg tctgagcaaa ttcatttgtt
    63841 aaacctgcac tagggcttga tacgaaattg cctaagtcgg tcccattgat cgataagtct
    63901 gtcatatcag aaaaataatc cgggtctttt tcaaagttat cgaatgttgc gcctgttttt
    63961 cgccataaag cgatttctga atgggcgatc tttaaagaag aatctccatt tccttggaat
    64021 acccagccgc ctatgccatt ggtctcgctt ccaatccgtt tgttttggaa atcaaaatag
    64081 gtaggagatt ctaagtccac attcaacaca tccccactga tgatgccggt cttatcttcc
    64141 cctgttttcc cgaccatgac taaataagtt ttttcacctg ccgtaaaatt taaatcaata
    64201 tctccagttg tatctacagt tgctccggaa tctgcgcgta tatcgatatt tgatttttct
    64261 ccagtgactg taaaatcagc tgaacctgga ctttctagaa aaccagaagc gtcataaaat
    64321 tggatcgctt gatttccttg attgagtctg ttcacgtaag atttgccatc acctgtattt
    64381 cttaccctca atgagcctcc attggttact gtcagctttt gcgtaccatt ctcaaaacga
    64441 actgcgctgg aacgcccact ctgcttatcg atgtcgataa ctgatccggc tccttctacg
    64501 atcaaagaaa ggccttttcc tacaaaacgc atcccattgt tcccgggttg atcgtcatct
    64561 ccagccattt tgaccgagat ttgtccacca cttgatacat ggatttgact agaatctcct
    64621 tgcaagcgaa atccgcccgt agttgctgta ttgatggtga ttttcccacc attcgataca
    64681 gtgactttcg atcctgaacc tctcatttgg atgattccgc gattgttgtc attggtaata
    64741 ttgatctttg tatttaccga aaattcgctt cccgaaccac tgacttcaaa catgacatta
    64801 ccaggtagcg tatcgactgc ctctccttca tcgctaacgc catctgcccg atcataatca
    64861 agagagacct ttgaaccgct taagatatgg attcctggcg ctgtttttcc actaccagtc
    64921 tcatctcctg aatctagaaa accgtagaat ttccctagat acggtccata aagttgacta
    64981 ccattgatca cattcaattg atagcttcta caaaaatagg ctttacccgg agcggcattc
    65041 agtccttcct tcactggttg gacatccaac acaccggaaa caccatctaa tgtgacagtt
    65101 gcacgaggag cgtaaatcag tcctaatttg gacccatcat acaaatcaag tgtcccggta
    65161 aattttattt ctcccgtaaa atgatatctg cttccccaaa aatcggtcat ataagcaatg
    65221 ataatggcat ttccatcgat atttggtgtc aatccacctg gatgtgcagc tgttccttta
    65281 aaattctgga actcgatcga ctgaggtatc tcattgatat aaatatggtg tgtgccgaaa
    65341 ttgtgagcga atccaccaaa atcaataacg atatctcgtt tgatctctaa ttccctgtct
    65401 aaagtcaaag gctgaatcag tcggatctta gttactgatt cgtctgcgag aagctgcgcc
    65461 agttcttctg ttgtggcagc ttcgctgtac ccatccgctg aaacagctct tgccagtgcc
    65521 gcttcactgc tatcagacgt ttgttcagct acttctgagg tctcacttgt cttattttct
    65581 tcttgccttt tattagtaga agcctcactt tgagattctg tagtttcaga agtaatcgat
    65641 tcttctgaaa aagtagtctt gtcaaaactt tgattctctg attgcgttga ttcgtttgtt
    65701 ttctctacat cggcaaaaac ggatgtcacc atcaagagag ggaagacaaa caaaatcgac
    65761 gcaatcagct tttttacaca ttgattcatt ctccaccatc ctctctgacg ttttgacaat
    65821 tttattttat cgttttaatc tatttccaat tgtactttca tatttaaaat atgtattttt
    65881 atggaaaaat ttttctatta ggtgtaaatt tcttttttgt acaaaaaaaa ggctgtgaca
    65941 aaagtcttgt cacagtccct tatcccgaac aagcggtgtt ctcccgccaa ctcctcggtc
    66001 acaacctcca ttttaacaat aaacctgtaa atgtgagggc aatactttga ttgtcacagg
    66061 taatttatcc ccttcatctc catcaacatt cgtcgtcaat tcttcctcat cagctaatga
    66121 cacaactaat tgctcacaag taaggtaggc tagatttttc gtagattcat ctactccttt
    66181 taataggtct ggcaatgttt taagcgtatc gatcaaagaa gaatctttta aataaaccaa
    66241 atggagctta ccatcatcca cttttgcatt tggaagtaat gtttcaaaac cgccgactga
    66301 gttagttagt cctatcagca aggtactgct ctcaatttct tccttctttc catcaacttc
    66361 tacgtgaaac gagtaggatt gtgtggaagc taactgtttg attcctgaca tgaaataagc
    66421 tagttttcca aatttcgtct tcttctccgc atctacatca ttgattgatt cagggataga
    66481 gccaattgcg acgacattca taaaatagtc gtcatttatt tttccaatat ccaatggctt
    66541 gactgattca atcgaaaaat gattgatcgc ttcctcaggt tctagcggta tccctaatgc
    66601 tcgagccaag tcattcaccg tacccaatgg gaaaaatccg aaatttggcc gatgttcttg
    66661 ttcagcgatt ccactgattc cttcgttgac tgttccatct ccgcccatga caaacacact
    66721 atgataccct tccgtagccg cttcgcgagt aaagtttttt gcatcccctg ctttttttgt
    66781 atgcaagacg accacttcat caaacaccga ttccaatttt gctatcgcta attgctcaaa
    66841 ttcctttgct tgttcaccgc ctgaactggg gtttaccact aataaaactt ttttcatcac
    66901 tttctttccc ttctgattga tttctttaat ataacgatag cataaagaaa aaaattaggc
    66961 tgtgatgaag tactgttcat gaaacattca tttatttttt gacaagataa aacatccata
    67021 ctatgaaact gaggaaaatt aaaacaatga tataggaaat aaaagaaaat gataggcatt
    67081 gtatacctaa taaagtaaaa atcttaggtg gcagataaaa acacaaaaat agcaagagaa
    67141 tgtaatagcg tttcttccac tttttgttat cagaccttat agcgatcaga ggaataccaa
    67201 atgcataact gaaaaactct tgatgtactg ttaatatgtc acggccttga ataataaatg
    67261 cgacaatccc cgctataatt agtgtgccta acaccaaaac ggaaaattga tgaatatatt
    67321 ttatatacaa atgatccatt gtacttcctg cctttttatt tgtgagaaaa acaaatccgt
    67381 atacgtgaat aacttctaga aataaaagaa atttgcaaaa ccactccatt tatctacttc
    67441 tacgtgcatt ttttgatatt gttttcttta tttaagatta tacagaaaaa tatctagggt
    67501 cgctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa gtagtaatta
    67561 aactactgtt gatttagttc taataaaaag atttagatga acagttttat tgatttaaat
    67621 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
    67681 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
    67741 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
    67801 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
    67861 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
    67921 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
    67981 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
    68041 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
    68101 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
    68161 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
    68221 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
    68281 caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt tctttggttg
    68341 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
    68401 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
    68461 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
    68521 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
    68581 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
    68641 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
    68701 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
    68761 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
    68821 tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg ttcgattttc
    68881 cagacatttt tttgttgttt ttattat
//
LOCUS       DAFEZB010000005        68228 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFEZB010000005
VERSION     DAFEZB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.005"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(137..1528)
                     /locus_tag="LP859_000379"
     CDS             complement(137..1528)
                     /locus_tag="LP859_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL3028360.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="LP859_000380"
     CDS             complement(1741..2127)
                     /locus_tag="LP859_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL3028361.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="LP859_000381"
     CDS             complement(2183..2806)
                     /locus_tag="LP859_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL3028362.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="LP859_000382"
     CDS             complement(2870..3538)
                     /locus_tag="LP859_000382"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL3028363.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="LP859_000383"
     CDS             complement(3550..3990)
                     /locus_tag="LP859_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028364.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="LP859_000384"
     CDS             complement(4049..4720)
                     /locus_tag="LP859_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3028365.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="LP859_000385"
     CDS             complement(4905..5303)
                     /locus_tag="LP859_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL3028366.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="LP859_000386"
     CDS             complement(5300..6073)
                     /locus_tag="LP859_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL3028367.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="LP859_000387"
     CDS             complement(6070..6753)
                     /locus_tag="LP859_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL3028368.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="LP859_000388"
     CDS             complement(6920..7807)
                     /locus_tag="LP859_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3028369.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="LP859_000389"
     CDS             complement(7804..8673)
                     /locus_tag="LP859_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028370.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="LP859_000390"
     CDS             complement(8810..10096)
                     /locus_tag="LP859_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028371.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="LP859_000391"
     CDS             10369..10947
                     /locus_tag="LP859_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL3028372.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="LP859_000392"
     CDS             10947..11876
                     /locus_tag="LP859_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028373.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="LP859_000393"
     CDS             11873..13609
                     /locus_tag="LP859_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL3028374.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="LP859_000394"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="LP859_000394"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL3028375.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="LP859_000395"
     CDS             complement(14505..15014)
                     /locus_tag="LP859_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL3028376.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="LP859_000396"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="LP859_000396"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL3028377.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="LP859_000397"
     CDS             complement(16315..17406)
                     /locus_tag="LP859_000397"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL3028378.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="LP859_000398"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="LP859_000398"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL3028379.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="LP859_000399"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="LP859_000399"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL3028380.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="LP859_000400"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="LP859_000400"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL3028381.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="LP859_000401"
     CDS             complement(21369..22343)
                     /locus_tag="LP859_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL3028382.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="LP859_000402"
     CDS             complement(22324..24207)
                     /locus_tag="LP859_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL3028383.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="LP859_000403"
     CDS             complement(24225..26450)
                     /locus_tag="LP859_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL3028384.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26565..27599
                     /locus_tag="LP859_000404"
     CDS             26565..27599
                     /locus_tag="LP859_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3028385.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27666..28679)
                     /locus_tag="LP859_000405"
     CDS             complement(27666..28679)
                     /locus_tag="LP859_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3028386.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28757..28945)
                     /locus_tag="LP859_000406"
     CDS             complement(28757..28945)
                     /locus_tag="LP859_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL3028387.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29385..29513)
                     /locus_tag="LP859_000407"
     CDS             complement(29385..29513)
                     /locus_tag="LP859_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL3028388.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="LP859_000408"
     CDS             complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="LP859_000408"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL3028389.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31000..32055)
                     /locus_tag="LP859_000409"
     CDS             complement(31000..32055)
                     /locus_tag="LP859_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL3028390.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="LP859_000410"
     CDS             complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="LP859_000410"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL3028391.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="LP859_000411"
     CDS             complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="LP859_000411"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL3028392.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="LP859_000412"
     CDS             complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="LP859_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL3028393.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="LP859_000413"
     CDS             complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="LP859_000413"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL3028394.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="LP859_000414"
     CDS             complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="LP859_000414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL3028395.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39817..41376
                     /locus_tag="LP859_000415"
     CDS             39817..41376
                     /locus_tag="LP859_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL3028396.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41487..43418)
                     /locus_tag="LP859_000416"
     CDS             complement(41487..43418)
                     /locus_tag="LP859_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3028397.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="LP859_000417"
     CDS             complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="LP859_000417"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL3028398.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSETETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44347..45099)
                     /locus_tag="LP859_000418"
     CDS             complement(44347..45099)
                     /locus_tag="LP859_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3028399.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45258..45467)
                     /locus_tag="LP859_000419"
     CDS             complement(45258..45467)
                     /locus_tag="LP859_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028400.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45478..46806)
                     /locus_tag="LP859_000420"
     CDS             complement(45478..46806)
                     /locus_tag="LP859_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028401.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            46942..47097
                     /locus_tag="LP859_000421"
     CDS             46942..47097
                     /locus_tag="LP859_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028402.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(47171..47758)
                     /locus_tag="LP859_000422"
     CDS             complement(47171..47758)
                     /locus_tag="LP859_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL3028403.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47841..49091)
                     /gene="clpX"
                     /locus_tag="LP859_000423"
     CDS             complement(47841..49091)
                     /gene="clpX"
                     /locus_tag="LP859_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL3028404.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49338..50627)
                     /locus_tag="LP859_000424"
     CDS             complement(49338..50627)
                     /locus_tag="LP859_000424"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL3028405.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50781..51713)
                     /locus_tag="LP859_000425"
     CDS             complement(50781..51713)
                     /locus_tag="LP859_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028406.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51870..52529)
                     /locus_tag="LP859_000426"
     CDS             complement(51870..52529)
                     /locus_tag="LP859_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL3028407.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52532..53557)
                     /gene="sppA"
                     /locus_tag="LP859_000427"
     CDS             complement(52532..53557)
                     /gene="sppA"
                     /locus_tag="LP859_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL3028408.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53570..53860)
                     /locus_tag="LP859_000428"
     CDS             complement(53570..53860)
                     /locus_tag="LP859_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028409.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54269..56074
                     /gene="glmS"
                     /locus_tag="LP859_000429"
     CDS             54269..56074
                     /gene="glmS"
                     /locus_tag="LP859_000429"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL3028410.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56146..57501)
                     /locus_tag="LP859_000430"
     CDS             complement(56146..57501)
                     /locus_tag="LP859_000430"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL3028411.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57525..58694)
                     /locus_tag="LP859_000431"
     CDS             complement(57525..58694)
                     /locus_tag="LP859_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028412.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58691..59578)
                     /locus_tag="LP859_000432"
     CDS             complement(58691..59578)
                     /locus_tag="LP859_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL3028413.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59767..63459)
                     /gene="nifJ"
                     /locus_tag="LP859_000433"
     CDS             complement(59767..63459)
                     /gene="nifJ"
                     /locus_tag="LP859_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL3028414.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63658..64179
                     /locus_tag="LP859_000434"
     CDS             63658..64179
                     /locus_tag="LP859_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL3028415.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(64878..65435)
                     /locus_tag="LP859_000435"
     CDS             complement(64878..65435)
                     /locus_tag="LP859_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028416.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(65614..66225)
                     /locus_tag="LP859_000436"
     CDS             complement(65614..66225)
                     /locus_tag="LP859_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028417.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(66338..66595)
                     /locus_tag="LP859_000437"
     CDS             complement(66338..66595)
                     /locus_tag="LP859_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028418.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(67192..67470)
                     /locus_tag="LP859_000438"
     CDS             complement(67192..67470)
                     /locus_tag="LP859_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028419.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKK"
     gene            complement(67504..67755)
                     /locus_tag="LP859_000439"
     CDS             complement(67504..67755)
                     /locus_tag="LP859_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028420.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        19060 a        14750 c        12003 g        22415 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgcttttct tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacga
    26521 ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag
    26581 atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc
    26641 agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg
    26701 attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct
    26761 ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga
    26821 tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata
    26881 atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta
    26941 cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc
    27001 ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg
    27061 tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag
    27121 tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct
    27181 atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct
    27241 ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc
    27301 ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac
    27361 aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa
    27421 tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag
    27481 cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga
    27541 cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa
    27601 agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca
    27661 acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct
    27721 tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac
    27781 aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc
    27841 tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg
    27901 tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac
    27961 gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct
    28021 gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga
    28081 agtagatgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat
    28141 tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc
    28201 aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact
    28261 tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg
    28321 gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat
    28381 cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag
    28441 atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg
    28501 tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata
    28561 tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac
    28621 gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata
    28681 aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca
    28741 agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa
    28801 gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac
    28861 attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt
    28921 caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca
    28981 actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg
    29041 tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc
    29101 atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt
    29161 gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg
    29221 aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca
    29281 actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga
    29341 aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt
    29401 ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg
    29461 cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt
    29521 ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag
    29581 gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag
    29641 ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa
    29701 taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc
    29761 ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt
    29821 cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca
    29881 tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat
    29941 aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca
    30001 gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt
    30061 ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta
    30121 aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt
    30181 cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta
    30241 aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc
    30301 cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta
    30361 ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat
    30421 catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga
    30481 tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa
    30541 cttctggcat cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa
    30601 gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt
    30661 tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa
    30721 acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga
    30781 taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga
    30841 tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct
    30901 ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa
    30961 accggcactg accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt
    31021 ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt
    31081 tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc
    31141 aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat
    31201 cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc
    31261 ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg
    31321 tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt
    31381 cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa
    31441 tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc
    31501 ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt
    31561 cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa
    31621 ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat
    31681 gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct
    31741 aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt
    31801 aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc
    31861 agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga
    31921 atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg
    31981 taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac
    32041 gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt
    32101 cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc
    32161 aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat
    32221 ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc
    32281 aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat
    32341 ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt
    32401 caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat
    32461 caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc
    32521 agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg
    32581 gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat
    32641 ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact
    32701 agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt
    32761 ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc
    32821 taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa
    32881 ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc
    32941 cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc
    33001 gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc
    33061 ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc
    33121 ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc
    33181 gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt
    33241 atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa
    33301 tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc
    33361 tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg
    33421 cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac
    33481 tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct
    33541 gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg
    33601 atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga catacctgga
    33661 agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg
    33721 ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca
    33781 taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt
    33841 ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt
    33901 tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc
    33961 acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct
    34021 tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact
    34081 tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt
    34141 acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg
    34201 atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct
    34261 ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc
    34321 attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg
    34381 ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc
    34441 ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca
    34501 ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa
    34561 gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc
    34621 atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa
    34681 aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca
    34741 attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc
    34801 tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct
    34861 ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc
    34921 atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc
    34981 cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca
    35041 ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt
    35101 caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca
    35161 tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa
    35221 gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc
    35281 tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat
    35341 tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag
    35401 ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg
    35461 tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac
    35521 cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg
    35581 ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt
    35641 gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca
    35701 attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga
    35761 tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga
    35821 aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa
    35881 tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg
    35941 atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag
    36001 caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat
    36061 cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc
    36121 atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac
    36181 aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca
    36241 cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca
    36301 agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg
    36361 ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga
    36421 ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg
    36481 ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca
    36541 ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt
    36601 caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga
    36661 atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga
    36721 aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta
    36781 actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg
    36841 cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta
    36901 cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt
    36961 cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat
    37021 cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta
    37081 cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa
    37141 cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt
    37201 ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga
    37261 ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt
    37321 ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag
    37381 cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca
    37441 attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt
    37501 tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt
    37561 ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga
    37621 acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt
    37681 tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca
    37741 aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt
    37801 aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc
    37861 agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc
    37921 aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg
    37981 gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc
    38041 gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg
    38101 aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat
    38161 gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat
    38221 tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca
    38281 cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca
    38341 agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg
    38401 atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc
    38461 agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct
    38521 ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac acgagattgt
    38581 gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct
    38641 tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa
    38701 gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg
    38761 ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct
    38821 agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct
    38881 gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat
    38941 ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct
    39001 acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat
    39061 aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct
    39121 ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt
    39181 gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt
    39241 cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc
    39301 agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct
    39361 cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca
    39421 gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta
    39481 atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt
    39541 gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg
    39601 tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac
    39661 attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt
    39721 aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg
    39781 atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag
    39841 aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca
    39901 ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt
    39961 atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc
    40021 cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg
    40081 attccacaag tagtgggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta
    40141 tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa
    40201 ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt
    40261 ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca
    40321 ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct
    40381 ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac
    40441 tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat
    40501 tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat
    40561 gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg
    40621 ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga
    40681 ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt
    40741 ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg
    40801 attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg
    40861 gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt
    40921 tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt
    40981 ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat
    41041 gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact
    41101 cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt
    41161 tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct
    41221 atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat
    41281 attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct
    41341 aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca
    41401 acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg
    41461 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
    41521 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
    41581 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
    41641 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
    41701 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
    41761 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
    41821 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
    41881 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
    41941 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
    42001 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
    42061 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
    42121 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
    42181 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
    42241 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
    42301 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
    42361 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
    42421 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
    42481 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
    42541 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
    42601 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
    42661 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
    42721 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
    42781 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
    42841 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
    42901 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
    42961 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
    43021 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
    43081 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
    43141 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
    43201 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
    43261 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
    43321 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
    43381 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
    43441 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
    43501 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
    43561 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
    43621 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
    43681 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
    43741 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
    43801 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
    43861 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
    43921 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
    43981 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
    44041 agccattgat ctcagtttca gtttctgatt ttagtttgat attgattctt gtatcttctt
    44101 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
    44161 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
    44221 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
    44281 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
    44341 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
    44401 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
    44461 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
    44521 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
    44581 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
    44641 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
    44701 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
    44761 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
    44821 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
    44881 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
    44941 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
    45001 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
    45061 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
    45121 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
    45181 tataaatcag tcaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg
    45241 tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca
    45301 gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg
    45361 accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat
    45421 aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca
    45481 tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag
    45541 atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt
    45601 gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc
    45661 tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat ctgttacctc
    45721 tacttctaga tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac
    45781 agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa
    45841 atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat
    45901 atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt gcatttgtgc
    45961 attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg
    46021 tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa
    46081 ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc gataaagctg
    46141 gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa
    46201 aatcggttta gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt
    46261 gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac
    46321 ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa
    46381 tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata
    46441 ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt
    46501 ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg
    46561 aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc
    46621 tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga
    46681 gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg
    46741 ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
    46801 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
    46861 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
    46921 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
    46981 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
    47041 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
    47101 aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg gtaagtcttt
    47161 tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct tcttttccct
    47221 gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc aacgcttttt
    47281 tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc ttagtggcaa
    47341 caacaattac agggatatca taatacttca aaaattgata catctggata tcttcttgag
    47401 aaggcttatg acgcaaatct actaaagaaa cgaccgcacg tagctggtct ctagtcgtta
    47461 aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact tttgcatacc
    47521 catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct
    47581 gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc aatgtgttga
    47641 taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc aattgtgtat
    47701 ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg actttcatgg
    47761 tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc caaaatgact
    47821 tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata gattactgtt
    47881 ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat gttttcattt
    47941 gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg aagtcctcga
    48001 gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc aggttcgaat
    48061 tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag cgcatttttc
    48121 ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc tgccataaca
    48181 ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc aggaatgata
    48241 tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc
    48301 ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc acctacgatg
    48361 aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt acgtccgccc
    48421 tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg ttggacacct
    48481 tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat tttatcgatc
    48541 tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc agattgaagc
    48601 aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt caaacttgtc
    48661 gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc taaaaatgtt
    48721 ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc aacatcatct
    48781 gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc aacagctaac
    48841 gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag aatttctagt
    48901 ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt
    48961 tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc tacgatcttt
    49021 ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt gctacttgga
    49081 ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac tatcaaagac
    49141 tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt taacaaagga
    49201 tcaactgacc tatacctttg cattttttac tcttaacaaa aaataaggaa ctgtgacaaa
    49261 atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt cttcagaaat
    49321 gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat caattcgatt
    49381 gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg tttgatttgt
    49441 tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat ttcttcttct
    49501 gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt tcttgtgcgc
    49561 atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc agttaattga
    49621 tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc gtccattgaa
    49681 cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc gttttcaaca
    49741 gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt tgtttctact
    49801 aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc atcaacatct
    49861 tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg gattgtcact
    49921 ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg gaaagttaca
    49981 tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa acctggaatg
    50041 aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg
    50101 ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc tgcagcatct
    50161 tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca
    50221 gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg tcctaatttt
    50281 acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt ttccatgctt
    50341 tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac agcagcttca
    50401 taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc accgtacata
    50461 cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt caagcttttt
    50521 ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat
    50581 tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta attccctccg
    50641 agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa atagtacact
    50701 aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca taacattttt
    50761 atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat tttttctctt
    50821 ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt tcctgtacac
    50881 gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc atagacagga
    50941 taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag cagcgcatct
    51001 tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg ctgctgcggc
    51061 tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa atagaaaaca
    51121 ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg
    51181 acctctggcg atacaataag tttactcgct gtttggataa actcatgata aggaagctgt
    51241 tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag ttcctccgat
    51301 ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc ttctgcaaat
    51361 tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact tgctatcaat
    51421 tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa ttcagcaaac
    51481 tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc ttcttttttc
    51541 tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct ttctttcaac
    51601 tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt
    51661 tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt cacgcagtgt
    51721 cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa agcatgacaa
    51781 ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt tagaaggtgc
    51841 tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt gaacgctttg
    51901 atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg ttgctttttc
    51961 tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag aatgaaccgg
    52021 caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc aaagctgatg
    52081 actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt cgtcaataaa
    52141 ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag ataagaatct
    52201 gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt aatagctccg
    52261 atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc atagaaatac
    52321 ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt tttccattta
    52381 cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc
    52441 ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc tttttcttgt
    52501 tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa tacattgctt
    52561 ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta gtctcagaag
    52621 gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca aacccagatg
    52681 gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt cgtatagctt
    52741 ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt gcttggacac
    52801 catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta ttccgtccct
    52861 cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga agcaccgctt
    52921 cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct ccgcttttaa
    52981 ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat cctgacatga
    53041 tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct gcttgagcag
    53101 aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt gggatatcat
    53161 gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag acaccgccac
    53221 caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca atcgccttca
    53281 gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct gaagaccctc
    53341 ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg tctccctctt
    53401 ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat ccctcaattt
    53461 gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca aataatcctg
    53521 cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc taatacaagt
    53581 tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct gctttttctt
    53641 cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta aactcacgag
    53701 cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag ccagtatcta
    53761 aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac aattgttttt
    53821 ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct cctctatccg
    53881 attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc attatttttt
    53941 gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt ttattctttt
    54001 tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa
    54061 aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
    54121 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
    54181 aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
    54241 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
    54301 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
    54361 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
    54421 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
    54481 acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
    54541 tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc
    54601 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
    54661 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
    54721 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
    54781 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
    54841 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
    54901 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
    54961 ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat
    55021 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
    55081 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
    55141 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt
    55201 ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac
    55261 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
    55321 cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
    55381 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
    55441 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
    55501 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
    55561 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
    55621 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc
    55681 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
    55741 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
    55801 accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
    55861 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
    55921 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
    55981 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
    56041 acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac
    56101 atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat
    56161 ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg
    56221 cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc
    56281 ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat
    56341 cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga ttgttacctc
    56401 tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc
    56461 tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc
    56521 gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg
    56581 tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat
    56641 gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca
    56701 gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg
    56761 atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc
    56821 taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt ctggatttgt
    56881 gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt
    56941 tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg
    57001 tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc
    57061 ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag
    57121 ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg
    57181 tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag
    57241 ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa
    57301 aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac
    57361 taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg
    57421 tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc
    57481 tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa
    57541 tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc
    57601 gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct
    57661 gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc
    57721 gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg
    57781 ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg
    57841 atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta
    57901 gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca
    57961 gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca
    58021 gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc
    58081 agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca
    58141 tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt
    58201 ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc
    58261 aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat
    58321 ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacttcatgt
    58381 gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt
    58441 gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca
    58501 tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac ccccgtaact
    58561 gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg
    58621 aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc
    58681 tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca
    58741 cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag attgccaaat
    58801 attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg acgtccccgg
    58861 tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg acagctgccc
    58921 ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga agataggcgc
    58981 ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag
    59041 gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca atccctgtct
    59101 caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg atttttcgct
    59161 tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt
    59221 cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt
    59281 ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc
    59341 aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta
    59401 cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa
    59461 gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac
    59521 cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa
    59581 aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag
    59641 gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa ttgcaagtat
    59701 gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt
    59761 tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc
    59821 tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg ttttttcttt
    59881 tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct tgattttttc
    59941 taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt ggacagtctt
    60001 ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa agcgaacttg
    60061 tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca aagtcattgg
    60121 gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat aaccagaaga
    60181 aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc catgtgtgat
    60241 acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt cttcaaatgc
    60301 cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa catttccata
    60361 tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag ccgaaaattt
    60421 agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg aatagacttc
    60481 gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat cgatcccgcc
    60541 aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt tttttacgaa
    60601 tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt cgctttcgat
    60661 cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca tgtgtactaa
    60721 ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt ctttgattga
    60781 acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac caaattcagc
    60841 attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat tggtcgtata
    60901 aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat tcgcgatcat
    60961 cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc cacagcctga
    61021 gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga cagtattctt
    61081 tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat tcacagcttg
    61141 ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg gacaagcctc
    61201 tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt attttacgcc
    61261 gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca tttcctcttc
    61321 atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt cattacacat
    61381 cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct tttccataga
    61441 agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg taccaacaga
    61501 gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat aaccagtgtt
    61561 ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga tcgcgacttg
    61621 atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga tagccattga
    61681 tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct catcaaaagg
    61741 aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc ggcgtcccaa
    61801 accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt tattttcagc
    61861 caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc tccaaagagt
    61921 attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc tatgcaggta
    61981 agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg aacgtatcgg
    62041 cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg aatcataata
    62101 aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag ctgatttatt
    62161 tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg tttgcggttc
    62221 tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat ccacgatccc
    62281 tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa aaactgctga
    62341 gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa taacagccgg
    62401 gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt ctccacctgc
    62461 ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag gcaacttttg
    62521 cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac ctgttttccg
    62581 tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg atcccataga
    62641 aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat aagaagttcc
    62701 tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt tatcataata
    62761 ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg taccactaac
    62821 agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct cctgattgat
    62881 cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt gactcgtacg
    62941 gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa gatgggcaac
    63001 aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa atcctgtttg
    63061 tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg tcgtcactgc
    63121 ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt acatattcgg
    63181 tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac cagttttcaa
    63241 tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta cttttactgg
    63301 ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt cagccatggt
    63361 cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag aaatatatgc
    63421 ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc cttgttcata
    63481 ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc ttttatttat
    63541 aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca aacagtctaa
    63601 atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg
    63661 gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca
    63721 tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta ttgccgacaa
    63781 tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga
    63841 gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt gatgcaccgg
    63901 tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat attcgatact
    63961 caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta
    64021 aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa
    64081 gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga tgaatctatt
    64141 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtgggaca
    64201 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    64261 actccttctt atttcttgga gttaaacgct tctgtcccag cctccttatt ccgaataaat
    64321 ggtgtccaaa agcttgcatc tgccagatta tctcatttct tatgacaagc taagcaaatt
    64381 atctccacca aataatttat tcatattttt gacttgcgca tactaactaa ttcttaaatt
    64441 ttcatacaaa atttctcaat ttcccttaac gagtaatatt tatgtacggt gattctcact
    64501 agtatcccaa tcacttttta agttatcccg atttcaattc tacttcaaaa atagttgctt
    64561 ttatgtatgc tgttagttga ttagatggtc atttgttgag ctaggtcgtt tattttaaac
    64621 aagttgtttc tttagcttat acctatctcg tctaccaaac gcaagatggc aaacacaaat
    64681 gcatacgcat ctgtatttgc cactttgatt cgtataaagc taacaactat taactcctct
    64741 cagctaatag ttgctctctt ttttattcag attcattttt gctgatcaca gcctagtatt
    64801 tcaatggttt tgaacactaa accaaattgt tccattacaa cattcactgg ctgatgaaat
    64861 ggattagtag atttcgatta ttcaaagcga ttttctattg gtggatcgtg ttcccaacta
    64921 ataagacgga ttccaaacct atcgtacaga tcttctaaac cacttataaa ttttgcacct
    64981 atttcaggtg ttttattgcg tggatattct attgttacgc cattaaatgg ataccaatta
    65041 ggattaggct ctcttttttt ctgtacatct cccatagaat agttagcact ttctgggaca
    65101 taatatattc ttcgaccatc ctctaattca tctaataaga tatttcgtga tcgtctatca
    65161 cttgctaaat gtagacgcca cgtgcgatac catgccatgc tacctgcatc tgcagaaaat
    65221 ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc tacttcatat
    65281 tctaatctaa aatcaaactc ccctcgggta atttctggtc ttggtggctt aggacccaat
    65341 attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac aacaataatg
    65401 attaatattt tgtttctatt tgataaattt ctcattattc gcacatcacc tctacattct
    65461 cacatattag ttcgtaatac tcctttaata tcaaaatatc tactggtcgt aataatattc
    65521 gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta cttgctgatg
    65581 atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt taacaatcgg
    65641 ttcagatatt tcccagttga ttaaggtaat tccgtattgt gtcaaaactt cttgcgcttc
    65701 tccgaaatta agtacagttc ttatgtatcc actttctcct aatataaagt ctacctctct
    65761 tttaaaacct tcctcgagat ttctaagggc tatatgtggt tttaatgaca ttgattgtac
    65821 attttccccc atataatatt gcgcacttcc tcgactaaaa tagattccta gatcgtcccc
    65881 aaaaggtaga tcttctctac cactagctaa tctacttctc catcttcgtt ctcttttcat
    65941 agaacctgcg ctgaactcac acccgttaaa ctcagctatg acagtatctt caattatgtt
    66001 aatctgtcca tctagttcgt attcaatcct aaatggaaat tctccatagg tgatttccgg
    66061 ttcgggtatt ttaggtgaga ttaatgcaaa gaaaggaaat attaagcttg gcaacatcaa
    66121 aaaaactcct cctatgatca caattcccga tgtaatccaa aatataattt gtacaattct
    66181 cattgatcca ttgtattcca ttttattccc cctattacta ttcatatccg cctatctata
    66241 tcgatcttcc aatgcagaga taattgcttc cataccttga ttttttactg agttacctat
    66301 gcgagatatc attctccact aaggtgcatc atattgtcta tctaacatga ttaattctcc
    66361 aataggtgta tactcctcca aaaacaacat ttaatgcatc tccctcgaca acataagcta
    66421 ccatgtttgc tgtatatgtg tttgagtcta attcataagc agataaattt actgtcgctg
    66481 gattaaatat aatcgcattc gtatttatag ctactgaatt tgctgcttca gcaccacctt
    66541 ttgaatgtcc gatcattgtg atttcaatag taggatttac gtggacagcc ctcaccgttt
    66601 accctgacca ttatagaaat cgcttacttt tttgaactac gcaacaaagg atacaatgct
    66661 cctaacttca ctgtctccat ttttgtccac taaggaatat aaaaaatagg cattaattca
    66721 tttaattcgg tcacaacacg ttacctttta aatcattata ctgtatctca tacattatta
    66781 tcaataaact attataaaaa tgcaaaaaat gctttatcaa tctattttgt aattggtcat
    66841 aatatcattg aaatcatacc ctgtatcgat ttttcctatg aatctcgtct atttgtgatt
    66901 gacgaaaaag aggcaagaca gacactggaa tggtaatttt acccactatt ttgctccaac
    66961 tatttgtaat cgtacccact tttgaaatac cgagatgatc attttaattg atatgctgat
    67021 tcccaagcaa aaccccaaga aattaaatgc tctatcgcat ctagcttctt gggttttgct
    67081 tatataaatt ccaacattta aattttttat gctgttcggt ctcagaaatg ccgtttttgc
    67141 ttatatcaca atatcaagat gtcaaattat tcgtgttgcc gatgactttg attatttttt
    67201 ttcaaattgt ttattaagac taatgctgtc tttttgcaga taccaatgtt cgaacttttt
    67261 aacattatca tatgatcctg caagttttaa cttcttacca ttagatgtta tcaaagttat
    67321 aattaacctt gcctttttcc cattaggatc ttttatttta tgaattttct gcaggtaatc
    67381 tctcctaatg gcagctattt ttacctcatc agtgctgata aaccattgat catccacata
    67441 catactgcct gttatgttgt gtttttccat ttcttccgag aacgaaaagt ttaaggctcg
    67501 ttcttacttc caaagttgaa tgagtccatg gattggactt agtgtgagaa ttgagaacaa
    67561 caataagaat ccatatcctc ccgcaaacat ccctaaggct atactgtctc tccatccata
    67621 agtaggatta gaagagaaaa gagtcaccgt ttgtaataat atgaaaagga ataatccagc
    67681 acaaacacca aagataacaa ctccaaacat tgaattttta tacaatgacc atttatttat
    67741 aggcttttct ttcattttac ttccccctac tgttctgttc acacggaatg aattcaaagt
    67801 tcataagccc tcctctttga acatatgaat acgacttgct aactatactt taaaaaagct
    67861 tgttttaagc ggttataggc gagttgtaat ctctattcat ctcattagca aataacttgc
    67921 cgaaaagtct actgaaatta aagcagatag tggaagaatt atttagccag gatagacact
    67981 atttgtgaaa cacatgtctt tacttttgaa attctatgat gaaaagcctt ggaatacagt
    68041 agctcttaat tggtaatact ttttccatta taaataacct cttttcattt tatatttatc
    68101 ccctctaaaa ataatatcta atctaatatt ttaaacttat atttccctcg ctgtgctaat
    68161 agagcattac taaataaata gaaactagaa aaaagcatta cgcctgccag aataaaccaa
    68221 ttatttgt
//
LOCUS       DAFEZB010000006        56192 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFEZB010000006
VERSION     DAFEZB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.006"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(294..839)
                     /locus_tag="LP859_000440"
     CDS             complement(294..839)
                     /locus_tag="LP859_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3028421.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(851..1543)
                     /locus_tag="LP859_000441"
     CDS             complement(851..1543)
                     /locus_tag="LP859_000441"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL3028422.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1562..1846)
                     /locus_tag="LP859_000442"
     CDS             complement(1562..1846)
                     /locus_tag="LP859_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028423.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1859..2422)
                     /locus_tag="LP859_000443"
     CDS             complement(1859..2422)
                     /locus_tag="LP859_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL3028424.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2650..3312
                     /locus_tag="LP859_000444"
     CDS             2650..3312
                     /locus_tag="LP859_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL3028425.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3436..4632
                     /locus_tag="LP859_000445"
     CDS             3436..4632
                     /locus_tag="LP859_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL3028426.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4733..6109)
                     /locus_tag="LP859_000446"
     CDS             complement(4733..6109)
                     /locus_tag="LP859_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3028427.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6146..7012)
                     /locus_tag="LP859_000447"
     CDS             complement(6146..7012)
                     /locus_tag="LP859_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL3028428.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(7101..8126)
                     /locus_tag="LP859_000448"
     CDS             complement(7101..8126)
                     /locus_tag="LP859_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3028429.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8144..8818)
                     /locus_tag="LP859_000449"
     CDS             complement(8144..8818)
                     /locus_tag="LP859_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3028430.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(9002..9895)
                     /locus_tag="LP859_000450"
     CDS             complement(9002..9895)
                     /locus_tag="LP859_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3028431.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9969..11666)
                     /locus_tag="LP859_000451"
     CDS             complement(9969..11666)
                     /locus_tag="LP859_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3028432.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11659..12972)
                     /locus_tag="LP859_000452"
     CDS             complement(11659..12972)
                     /locus_tag="LP859_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL3028433.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12972..17009)
                     /locus_tag="LP859_000453"
     CDS             complement(12972..17009)
                     /locus_tag="LP859_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL3028434.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(17022..17480)
                     /locus_tag="LP859_000454"
     CDS             complement(17022..17480)
                     /locus_tag="LP859_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3028435.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17778..18296)
                     /locus_tag="LP859_000455"
     CDS             complement(17778..18296)
                     /locus_tag="LP859_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL3028436.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18298..18915)
                     /locus_tag="LP859_000456"
     CDS             complement(18298..18915)
                     /locus_tag="LP859_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL3028437.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18912..19775)
                     /locus_tag="LP859_000457"
     CDS             complement(18912..19775)
                     /locus_tag="LP859_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3028438.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19762..20673)
                     /locus_tag="LP859_000458"
     CDS             complement(19762..20673)
                     /locus_tag="LP859_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028439.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20714..22048)
                     /locus_tag="LP859_000459"
     CDS             complement(20714..22048)
                     /locus_tag="LP859_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3028440.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22256..23086
                     /locus_tag="LP859_000460"
     CDS             22256..23086
                     /locus_tag="LP859_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL3028441.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23330..23648)
                     /locus_tag="LP859_000461"
                     /pseudo
     CDS             complement(23330..23648)
                     /locus_tag="LP859_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23770..23964)
                     /locus_tag="LP859_000462"
     CDS             complement(23770..23964)
                     /locus_tag="LP859_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028442.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24469..24654)
                     /locus_tag="LP859_000463"
     CDS             complement(24469..24654)
                     /locus_tag="LP859_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028443.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25504..25845)
                     /locus_tag="LP859_000464"
     CDS             complement(25504..25845)
                     /locus_tag="LP859_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3028444.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25845..26075)
                     /locus_tag="LP859_000465"
     CDS             complement(25845..26075)
                     /locus_tag="LP859_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3028445.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26362..26700
                     /locus_tag="LP859_000466"
     CDS             26362..26700
                     /locus_tag="LP859_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028446.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26905..27144)
                     /locus_tag="LP859_000467"
     CDS             complement(26905..27144)
                     /locus_tag="LP859_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL3028447.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(27120..27467)
                     /locus_tag="LP859_000468"
     CDS             complement(27120..27467)
                     /locus_tag="LP859_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL3028448.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(28199..28780)
                     /locus_tag="LP859_000469"
     CDS             complement(28199..28780)
                     /locus_tag="LP859_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3028449.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28963..29589)
                     /locus_tag="LP859_000470"
     CDS             complement(28963..29589)
                     /locus_tag="LP859_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3028450.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29611..30942)
                     /locus_tag="LP859_000471"
     CDS             complement(29611..30942)
                     /locus_tag="LP859_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL3028451.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(31097..31498)
                     /locus_tag="LP859_000472"
     CDS             complement(31097..31498)
                     /locus_tag="LP859_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL3028452.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31584..31790)
                     /locus_tag="LP859_000473"
     CDS             complement(31584..31790)
                     /locus_tag="LP859_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028453.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31885..33387)
                     /locus_tag="LP859_000474"
     CDS             complement(31885..33387)
                     /locus_tag="LP859_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL3028454.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33405..33860)
                     /gene="argR"
                     /locus_tag="LP859_000475"
     CDS             complement(33405..33860)
                     /gene="argR"
                     /locus_tag="LP859_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL3028455.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33958..34575)
                     /locus_tag="LP859_000476"
     CDS             complement(33958..34575)
                     /locus_tag="LP859_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL3028456.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34796..36169
                     /locus_tag="LP859_000477"
     CDS             34796..36169
                     /locus_tag="LP859_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3028457.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36244..37356
                     /locus_tag="LP859_000478"
     CDS             36244..37356
                     /locus_tag="LP859_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL3028458.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37428..39065)
                     /locus_tag="LP859_000479"
     CDS             complement(37428..39065)
                     /locus_tag="LP859_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL3028459.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(39233..39841)
                     /locus_tag="LP859_000480"
     CDS             complement(39233..39841)
                     /locus_tag="LP859_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3028460.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(40225..41376)
                     /gene="queG"
                     /locus_tag="LP859_000481"
     CDS             complement(40225..41376)
                     /gene="queG"
                     /locus_tag="LP859_000481"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL3028461.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(41395..42147)
                     /locus_tag="LP859_000482"
     CDS             complement(41395..42147)
                     /locus_tag="LP859_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028462.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(42288..43238)
                     /locus_tag="LP859_000483"
     CDS             complement(42288..43238)
                     /locus_tag="LP859_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL3028463.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(43239..44078)
                     /locus_tag="LP859_000484"
     CDS             complement(43239..44078)
                     /locus_tag="LP859_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL3028464.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(44075..44821)
                     /locus_tag="LP859_000485"
     CDS             complement(44075..44821)
                     /locus_tag="LP859_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL3028465.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            45059..46429
                     /locus_tag="LP859_000486"
     CDS             45059..46429
                     /locus_tag="LP859_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3028466.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(46484..47380)
                     /locus_tag="LP859_000487"
     CDS             complement(46484..47380)
                     /locus_tag="LP859_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3028467.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(47494..47664)
                     /locus_tag="LP859_000488"
     CDS             complement(47494..47664)
                     /locus_tag="LP859_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028468.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(47661..48338)
                     /locus_tag="LP859_000489"
     CDS             complement(47661..48338)
                     /locus_tag="LP859_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL3028469.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(48429..49334)
                     /locus_tag="LP859_000490"
     CDS             complement(48429..49334)
                     /locus_tag="LP859_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL3028470.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIEVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            49509..50963
                     /locus_tag="LP859_000491"
     CDS             49509..50963
                     /locus_tag="LP859_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3028471.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            50979..52010
                     /locus_tag="LP859_000492"
     CDS             50979..52010
                     /locus_tag="LP859_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL3028472.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            52017..53204
                     /locus_tag="LP859_000493"
     CDS             52017..53204
                     /locus_tag="LP859_000493"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL3028473.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            53360..54742
                     /locus_tag="LP859_000494"
     CDS             53360..54742
                     /locus_tag="LP859_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL3028474.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            54869..55099
                     /locus_tag="LP859_000495"
     CDS             54869..55099
                     /locus_tag="LP859_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028475.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(55299..55967)
                     /locus_tag="LP859_000496"
     CDS             complement(55299..55967)
                     /locus_tag="LP859_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL3028476.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        16200 a        11715 c         9951 g        18326 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taaattataa taagtgtatt
      121 ttataaaaag aaaaaatata acttaagttg ttgataataa agagatttat taacggaaaa
      181 agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc gtttgtttta
      241 tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt tttttataaa
      301 atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg
      361 aaacttgcca cggaatcttt gaaaatatgg agtttgtatc ctagattata cgcatcgact
      421 gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc tgtgatctgg
      481 cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg ccgtttgtct
      541 atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa agagccgtac
      601 aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt ctccggatga
      661 aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata
      721 aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt cccagtagtc
      781 agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc
      841 ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct gatcgtttgc
      901 ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca
      961 ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca acagcggctc
     1021 cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg attttatctg
     1081 gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc aaattcgttg
     1141 cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa tatagaggca
     1201 ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc atctgcatca aaataagcta
     1261 ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg ccagctgaac
     1321 ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg gtaatagatg
     1381 ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct aatgaacctg
     1441 aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc tctctcaaaa
     1501 tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta attccttctt
     1561 tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca ataataagag
     1621 atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc cttgttttcg
     1681 tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt ttttcttctt
     1741 gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt
     1801 gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc cgatccctct
     1861 actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa
     1921 tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac aactccagta
     1981 cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt gcatgataaa
     2041 tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga tggctcagcg
     2101 acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt tcgggatttt
     2161 gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt tctaaaggtt
     2221 ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg atcccgacac
     2281 cgccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa cgaacttcgt
     2341 ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt gttttttctt
     2401 caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata actgcttttt
     2461 tttcattttt caatttttct caaaaactcc ttattagttt accatagata aaccttagat
     2521 tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag tccgtatgct
     2581 aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga
     2641 gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc
     2701 tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg tgtagtttta
     2761 gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca
     2821 ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa tgaaatttca
     2881 ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca aatgaatatg
     2941 cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa agcagcgaaa
     3001 gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag tcagtttctt
     3061 tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat caatgaccat
     3121 gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag
     3181 ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct agatgatatc
     3241 gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata tgaaccagaa
     3301 caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat gatgtctggc
     3361 tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa cagattcaac
     3421 aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac accagttaac
     3481 gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac gctgaccact
     3541 tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg tttaattgac
     3601 aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat cgatgctaat
     3661 gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag tgaattctca
     3721 caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga ttccttaact
     3781 gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg agaagggaac
     3841 atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac agcaaaatat
     3901 caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga aaaagacggt
     3961 ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga ctactttgat
     4021 gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca aacaacgatc
     4081 attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt tcagattgcc
     4141 aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct tcgcttagct
     4201 agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac aaatcaggcc
     4261 ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa aaaccaagtc
     4321 gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct
     4381 gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa
     4441 acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac cactcaaaat
     4501 gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa agaaaaacgt
     4561 cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa actgttggat
     4621 ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta
     4681 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
     4741 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
     4801 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
     4861 agatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
     4921 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
     4981 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
     5041 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
     5101 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
     5161 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
     5221 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
     5281 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
     5341 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
     5401 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
     5461 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
     5521 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
     5581 aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
     5641 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
     5701 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
     5761 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
     5821 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
     5881 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
     5941 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
     6001 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
     6061 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
     6121 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
     6181 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
     6241 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
     6301 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
     6361 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
     6421 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
     6481 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
     6541 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
     6601 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
     6661 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
     6721 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
     6781 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
     6841 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
     6901 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
     6961 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
     7021 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
     7081 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
     7141 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
     7201 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
     7261 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
     7321 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
     7381 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
     7441 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
     7501 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
     7561 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
     7621 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
     7681 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
     7741 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
     7801 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
     7861 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
     7921 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
     7981 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
     8041 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
     8101 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
     8161 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
     8221 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
     8281 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
     8341 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
     8401 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
     8461 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
     8521 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
     8581 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
     8641 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
     8701 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
     8761 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
     8821 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
     8881 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
     8941 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
     9001 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
     9061 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
     9121 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
     9181 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
     9241 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
     9301 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
     9361 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
     9421 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
     9481 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
     9541 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
     9601 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
     9661 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
     9721 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
     9781 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
     9841 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
     9901 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
     9961 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
    10021 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
    10081 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
    10141 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
    10201 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
    10261 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
    10321 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
    10381 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
    10441 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
    10501 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
    10561 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
    10621 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
    10681 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
    10741 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
    10801 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
    10861 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
    10921 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
    10981 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
    11041 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
    11101 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
    11161 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
    11221 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
    11281 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
    11341 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
    11401 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
    11461 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
    11521 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
    11581 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
    11641 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
    11701 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
    11761 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
    11821 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
    11881 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
    11941 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
    12001 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
    12061 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
    12121 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
    12181 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
    12241 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
    12301 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
    12361 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
    12421 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
    12481 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
    12541 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
    12601 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
    12661 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
    12721 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
    12781 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
    12841 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
    12901 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
    12961 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
    13021 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
    13081 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
    13141 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
    13201 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
    13261 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
    13321 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
    13381 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
    13441 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
    13501 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
    13561 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
    13621 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
    13681 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
    13741 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
    13801 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
    13861 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
    13921 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
    13981 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
    14041 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
    14101 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
    14161 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
    14221 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
    14281 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
    14341 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
    14401 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
    14461 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
    14521 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
    14581 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
    14641 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    14701 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    14761 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    14821 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    14881 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    14941 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    15001 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    15061 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    15121 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    15181 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    15241 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    15301 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    15361 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    15421 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    15481 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    15541 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    15601 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    15661 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    15721 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    15781 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    15841 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    15901 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    15961 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    16021 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    16081 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    16141 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    16201 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    16261 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    16321 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    16381 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    16441 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    16501 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    16561 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    16621 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    16681 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    16741 aacttcgggt agcgatgctg ttcgttttca acttctccgc taggatagat atattcatag
    16801 gggtcttgcg tagctgttgg cccaagacgc tccaaaatac cgttgaataa ctggcgttga
    16861 atgcgctgga gtgcttgacc gctgggtacg attggttcgt ccagcttttc catctcattt
    16921 tcttcgctac gttgaatttc agcggaatct tctgtatcag tttcattttc ttgtaattct
    16981 tggttatcta ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg
    17041 gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt
    17101 ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca
    17161 taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca
    17221 tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc
    17281 cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct
    17341 aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata
    17401 tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc
    17461 ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca
    17521 acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa
    17581 atgatatcct ttttagttaa aaaacaccaa ttatacaaca tttattatta aaattaaatt
    17641 ataaaatttt ataacgttct catttaacaa aacttaagtt attaagacaa atgaataaac
    17701 aactacgctt tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg cactcatgct
    17761 ttattcgtac aattaattca gataacggca gcgcatagga tgccaataac acttcctaaa
    17821 atagctcctc ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct cgaataagcg
    17881 atcaaacaag caaataaaaa ggctaatatt cctgcaactg gacttaattc gaacaacgca
    17941 gctactgata aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt cttcggttta
    18001 cttgtgtaaa ggacaagccg acgatttttt tcataaggtc ttttccgtct tgtcgttttt
    18061 ttgataaaaa gcaaaccaac aaaaaaatag atcaaactgc cgataagaat ggctagacca
    18121 tattctctag tatcaggtat caagactaaa acagcagcaa ggagcaacca aataaaacca
    18181 ccattagaga tattactgat aaatttcaaa aaattgattt ttcgactatc cgttttccgt
    18241 tgcttggcga acttgttgat aatactatca tcgattttag caatcaaacg caacatgtta
    18301 ttcctccttt ttaagaacct tttcttgcgt acgatggata aaaagcagga cttgaaaata
    18361 aaacaagaaa ctgaaaccta ataaagcaaa atagattggt cctgcaaacc agaatgcgac
    18421 aagaaggatc aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa
    18481 gaaaaatgca gtaatccaag aagcttcttt ttgttccagt tgaagcaaga ttttgtaact
    18541 aaagagaagc gcgcttaata gaagcaaaaa caacgtagta tacatcaatg tatagccaat
    18601 tcccccaccc caatagcgga aaatctgata atcgatcaat agaagaagaa aataaacact
    18661 tgctaaacta tcttgctttc cataaagaga gaagttctca cgaaactttt tccagaatag
    18721 tgaaccgagc tttttctctt catttttttt gtaatgataa atcatataca gaactgttcg
    18781 acttgcagtt cctaatgtga aaacaggcaa gacgcacagg aaccatagta gctgtaaatg
    18841 gataagacgt ggaatccatt caatgatttc taagaaataa tcattggctt ttttaccaat
    18901 cgatcgattc atcatccttt tgaacctcct gtcaaatcca tcccttcaat gaaataacgt
    18961 tgtgcaaaaa agaacagcaa aatcgtcggg atcaagacaa gtgttgctcc tgccatcaga
    19021 taattccatt gtgtcgttgc ttggttctga aaaacttgca agccaatctg taacgtgtat
    19081 ttttcttgat cttggatata taacagagga cctaagaaat cattccaagc tgcgttgaag
    19141 gaattgatcg caatggtaat caaggcaggt ttcgtcaatg gaagcatcaa atgactccaa
    19201 atatacaaat gattcgctcc gtcgatttca gcagcttcga tcaattcatt attgatcgat
    19261 aagtaaaatt gacgcatcag aaatatattg aacgcattgc cgaagaacga tggaacgatc
    19321 aggggcaaat aagtacctac ccagccgatc ttggagaata atacatattg agggatcatc
    19381 gttacaaacc ccgggatcat catcgtagaa agaaccaaag caaacatcag ctttttccca
    19441 ggaaaatcaa gcttcgcaaa tccataagca ataaaagaat ttgaaaggac attaccgatc
    19501 actactagca cagtgatgaa taatgtattt cttgcatagc gccaaaaagg aaaggcggca
    19561 atcgtatcca gatagttttc ccatacgatt ttttctggga aaaaggtcgg aggataacga
    19621 acgatttctt ccattggttt caatgaagta aacaccatcc aaagcaatgg aatgatcaaa
    19681 atgattccta ttactgtcat gaggacataa cttagtattt ttttgtttgc cgattcacac
    19741 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
    19801 tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
    19861 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
    19921 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
    19981 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
    20041 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
    20101 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
    20161 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
    20221 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
    20281 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
    20341 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
    20401 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
    20461 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
    20521 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
    20581 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
    20641 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
    20701 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
    20761 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
    20821 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
    20881 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
    20941 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
    21001 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
    21061 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
    21121 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
    21181 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac
    21241 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
    21301 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
    21361 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
    21421 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
    21481 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
    21541 taaatatctt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
    21601 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
    21661 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
    21721 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
    21781 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
    21841 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
    21901 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
    21961 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
    22021 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
    22081 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
    22141 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
    22201 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
    22261 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
    22321 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
    22381 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
    22441 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
    22501 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
    22561 tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga
    22621 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
    22681 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
    22741 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
    22801 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
    22861 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
    22921 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
    22981 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
    23041 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
    23101 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
    23161 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
    23221 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
    23281 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
    23341 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
    23401 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
    23461 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
    23521 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
    23581 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
    23641 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
    23701 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
    23761 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
    23821 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
    23881 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
    23941 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
    24001 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
    24061 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
    24121 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
    24181 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
    24241 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
    24301 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
    24361 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
    24421 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
    24481 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
    24541 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
    24601 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
    24661 acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag
    24721 tgactcaaaa taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct
    24781 aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga
    24841 tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct
    24901 ttgagattct actttggttc actatctgac ccaattgcac attttcgatc attacaagta
    24961 ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa
    25021 tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat
    25081 gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga
    25141 tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa
    25201 gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt
    25261 attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca
    25321 aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt
    25381 tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact
    25441 tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa
    25501 tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt
    25561 ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac
    25621 ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt
    25681 ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac
    25741 gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca
    25801 tacaatatca ccttttttag gaatataaat tctttcttct gacactataa aatttctctc
    25861 ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca
    25921 ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg
    25981 atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca
    26041 gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta
    26101 tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt
    26161 gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct
    26221 ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac
    26281 tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca
    26341 aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc
    26401 tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt
    26461 cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa
    26521 gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat
    26581 gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat
    26641 acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa
    26701 tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga
    26761 agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc
    26821 atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc
    26881 tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg
    26941 agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt
    27001 caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat
    27061 tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac
    27121 tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa
    27181 acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca
    27241 cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc
    27301 ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact
    27361 tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa
    27421 ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc
    27481 aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt
    27541 tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg
    27601 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt
    27661 gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa
    27721 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa
    27781 atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    27841 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta
    27901 tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc actaaacggt
    27961 gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    28021 gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt
    28081 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat
    28141 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt
    28201 aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta
    28261 gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg
    28321 ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat
    28381 tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt
    28441 catccactgt actcaactca ataatcaaat tacctacagg acagccatga taagtgatct
    28501 gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt
    28561 ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga
    28621 tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg
    28681 tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa
    28741 gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt
    28801 tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa
    28861 gactgtgcca aattatctga cacagccctc actataaaac tgttctcata tagctagatg
    28921 ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa
    28981 tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc
    29041 cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact
    29101 ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat
    29161 tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa
    29221 gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat
    29281 aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg
    29341 ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta
    29401 aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc
    29461 aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc
    29521 gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt
    29581 attgaacatg gtggattctc cattcgttat ttattttaat acatcattta gagcttcact
    29641 cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat
    29701 agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag
    29761 aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac
    29821 ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc
    29881 tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta
    29941 aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat
    30001 gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat
    30061 cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc
    30121 tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt
    30181 tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac
    30241 ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat
    30301 tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag
    30361 aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc
    30421 aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg
    30481 atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa
    30541 aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc
    30601 accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac
    30661 tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa
    30721 tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa
    30781 tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt
    30841 ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc
    30901 ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt
    30961 ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc caaatcatct
    31021 gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc
    31081 gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt
    31141 tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga
    31201 tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca tctaaattat
    31261 aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat
    31321 gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg
    31381 ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct
    31441 cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg
    31501 tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa
    31561 ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt
    31621 ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata
    31681 gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag
    31741 ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc
    31801 ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc
    31861 aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg
    31921 tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc
    31981 tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc
    32041 gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc
    32101 tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg
    32161 ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt
    32221 gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt
    32281 tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct
    32341 tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat
    32401 tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct
    32461 ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa
    32521 tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact
    32581 gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc
    32641 taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt
    32701 aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt
    32761 caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca
    32821 accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc
    32881 ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat
    32941 tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt
    33001 aaagaatttt attccattat aataataggg aagatggcta gcagtcagca taaccgcagc
    33061 atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc
    33121 gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc
    33181 tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt
    33241 tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc
    33301 tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc
    33361 attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt
    33421 tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta
    33481 tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt
    33541 accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata
    33601 cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc
    33661 tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt
    33721 tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc
    33781 gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct
    33841 tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt
    33901 aatatttctt catattttgg tattttttac ttttattttt tgttttcatt caggttgcta
    33961 acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg
    34021 atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat
    34081 gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat
    34141 ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg
    34201 aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg
    34261 tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt
    34321 ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat
    34381 cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac
    34441 tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt
    34501 tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca
    34561 ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc
    34621 tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag
    34681 tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta
    34741 tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac
    34801 aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc
    34861 gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga
    34921 tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat
    34981 tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc
    35041 aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct
    35101 attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca
    35161 tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt
    35221 gacaacggcc attgctccag caattggccc cacttatgga ggcattatga cttcacattt
    35281 tacatggaat catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg
    35341 ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt
    35401 tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt
    35461 ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag
    35521 taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt
    35581 gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc
    35641 gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc
    35701 cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg
    35761 tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt
    35821 cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat
    35881 cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga
    35941 actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc
    36001 tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga
    36061 agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt
    36121 aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac
    36181 ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt
    36241 tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac
    36301 ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa
    36361 gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt
    36421 ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa
    36481 acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg
    36541 gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt
    36601 tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc
    36661 agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca
    36721 caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg
    36781 ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg
    36841 gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg
    36901 cagctcttag catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt
    36961 gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat
    37021 gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg attgactaag
    37081 agtacagaac agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt
    37141 gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat
    37201 ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc
    37261 ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag
    37321 gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga
    37381 tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc
    37441 tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga
    37501 gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta
    37561 tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca
    37621 aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca
    37681 ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt
    37741 ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga
    37801 ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg
    37861 tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc
    37921 ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg
    37981 ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg
    38041 tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca
    38101 ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta
    38161 tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca
    38221 tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat
    38281 tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat
    38341 tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc
    38401 gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta
    38461 tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca
    38521 caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc
    38581 ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct
    38641 ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta
    38701 tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg
    38761 ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg
    38821 cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt
    38881 gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc
    38941 ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga
    39001 atactggata acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta
    39061 ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga
    39121 gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
    39181 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
    39241 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
    39301 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
    39361 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
    39421 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
    39481 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
    39541 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
    39601 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
    39661 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
    39721 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
    39781 acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca
    39841 tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta
    39901 gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga attatttaat
    39961 atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct tgttgtataa
    40021 gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag attctgtaac
    40081 attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat caaattctct
    40141 taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac tggacaaaaa
    40201 ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt tctgggcatg
    40261 ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat ctaatacatc
    40321 aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg ctgttcctct
    40381 gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg ttcgatcacc
    40441 tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac gccatgaacc
    40501 agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca ttggctttaa
    40561 ttttggatat atttcgtcta cttttggttc catctcttca tgaaaatgaa aatcttttcc
    40621 ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat ttcgcatttt
    40681 tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca aacatcgctg
    40741 ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca cgcagcgcat
    40801 acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat tagtaacgat
    40861 ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt ttcgtccaat
    40921 aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag tatctacttg
    40981 cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt ggatgaattc
    41041 gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc aagaagcacg
    41101 agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag taggatacgc
    41161 aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat aaagacgctc
    41221 atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt cttccaacga
    41281 ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa ttcctaatcg
    41341 tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt gctgttaacg
    41401 gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga tctgctgatc
    41461 ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc aataatttgt
    41521 tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa ctggttttgt
    41581 acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca gatcgcggga
    41641 atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt gcgggatttt
    41701 cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt tgctgttggc
    41761 aataaattga cttttataat aggccgactg gatctttttg attactcctt ctgctttgat
    41821 gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt taactaatac
    41881 gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg tattttcatc
    41941 taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat ctggttctgt
    42001 tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa attgaaggtc
    42061 ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt ctttaatcgt
    42121 atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc taactataac
    42181 ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt tttcatatct
    42241 cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca gctttctttt
    42301 gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg atagtaagaa
    42361 tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata gattttcaaa
    42421 cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc cacgaataaa
    42481 accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc aggcgtacct
    42541 tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc tcgtgaaaaa
    42601 tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat agaagcaaaa
    42661 ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc tgcatttttt
    42721 ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat ttcctccaca
    42781 tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt atgttcttca
    42841 ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc ttttccttct
    42901 tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc aagattcaaa
    42961 ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg tagtacttca
    43021 tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg atctgttccg
    43081 acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa cttttcttta
    43141 cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac caagaaacta
    43201 actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc tttctgaaat
    43261 aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt acatgctaac
    43321 acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc gacaactgca
    43381 cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt cgttagcaag
    43441 taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc tactgtctgt
    43501 aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat cagcgatgtg
    43561 ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa ttctttataa
    43621 aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa caaatcgctt
    43681 tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc actctgggca
    43741 tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc aatcgctgta
    43801 tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt tagcatccca
    43861 aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc tacacctgga
    43921 agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat aataaaagaa
    43981 ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta ttgcaggaat
    44041 tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct ccttttacta
    44101 aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag acttcttgta
    44161 ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa tattcttcta
    44221 ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc aattcctgca
    44281 agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt tcatctaaaa
    44341 agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga agctgtccac
    44401 ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt ttcaaagctt
    44461 gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa gatggatatg
    44521 ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat tcacttcgct
    44581 gttccacgta ggctattttt ttacggactg agtttaccga ctgtccagaa aaggtgactt
    44641 taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat tttcctgcac
    44701 cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg atagttttca
    44761 aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc gtacgttcca
    44821 aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt taactaaaat
    44881 ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct cgcattttat
    44941 gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata atgaaaaaca
    45001 tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga gtttttgtat
    45061 ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga tcgcactagg
    45121 tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt ggacagggcc
    45181 ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta tgcgtgcttt
    45241 aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg cgagcgagta
    45301 catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat atattgtcgt
    45361 aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc ctgatttgcc
    45421 ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc ttatttctgt
    45481 caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa cgatcatttt
    45541 gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg gtcaggcagt
    45601 agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca aaggattttt
    45661 ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg gaatgactgc
    45721 tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca ctatcggtcg
    45781 aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc cgtggaatca
    45841 attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca ttacatttgc
    45901 tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca attcagggat
    45961 tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt ctaaaaaatt
    46021 tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct caggtggaat
    46081 cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt cagaaaatat
    46141 tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg tagtgatttt
    46201 gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc atccttttaa
    46261 aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta tcgtattgat
    46321 tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg ttttcttgct
    46381 ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa aaaagaacga
    46441 agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc aattttagcg
    46501 tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac aagatcgttt
    46561 ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc aaaactacgg
    46621 ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct gttgtgtctt
    46681 gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca gaatcagaag
    46741 atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc tgcaaataaa
    46801 tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg atcaaagtat
    46861 ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat tttctgatcc
    46921 cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct gataaaccag
    46981 ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat gcaggcgtct
    47041 tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct ggcaaaccat
    47101 ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac tcacttgttt
    47161 gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc gcttctgacg
    47221 aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa aacacggcaa
    47281 ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt cttcttcggg
    47341 tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc accaattttt
    47401 atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat tgtcgctgtg
    47461 cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat tccaagattg
    47521 tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca tcagtttctt
    47581 tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt catctaattg
    47641 ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag tgtaaaggtc
    47701 aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc ttgtttttca
    47761 gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca gtcaatccgc
    47821 tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat tagcttctcc
    47881 caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg aagataggtc
    47941 atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat cgtatattct
    48001 tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac gattcctagt
    48061 tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca aatttttaaa
    48121 tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc atgctgggcc
    48181 aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga ttccaaatga
    48241 tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt taaagattct
    48301 tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc tttttagtct
    48361 tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg tcattttaac
    48421 cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt tttcaaggta
    48481 tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt cgtagccgtt
    48541 tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa ccctacttga
    48601 taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc tcgattgatc
    48661 cgtacctcga tcaaatattc cttgatactg gtacccatat gatttttgaa tactcgcgac
    48721 aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa tttttcagca
    48781 taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac cttttgtaca
    48841 ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta ggttaatgct
    48901 gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc taaaataatg
    48961 tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct gaaaagcgac
    49021 tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata catccacgga
    49081 tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa cagatcacct
    49141 tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc catcacgaca
    49201 tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat cgttttagag
    49261 aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt ttttaaaaaa
    49321 atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca aaaagttaaa
    49381 taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta agtcattaaa
    49441 agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac aggagaggaa
    49501 tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc gcttttggac
    49561 acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca agccagatgt
    49621 tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca agcttagttg
    49681 taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc atcgataata
    49741 ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt gttagttccg
    49801 tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat acgactttat
    49861 ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca tttaaggata
    49921 tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt gaaaaatttg
    49981 ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta gtcgttgtcc
    50041 ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc gcttcaggct
    50101 ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt atcactgcct
    50161 tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct gggatcaaag
    50221 atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt gcttatatcg
    50281 cttatgcgat tgccaatgca gcaacaactg ccgtattatt ctacttcttc aaatatgtac
    50341 ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt ggtttagtag
    50401 ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt taccttggag
    50461 ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca agtttgccaa
    50521 tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc ttatccgctt
    50581 tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc cgtaacgaag
    50641 ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg tctaacggga
    50701 tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct gctgatatca
    50761 ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct gctttgatga
    50821 ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa catgcacaaa
    50881 tcgttgagaa attggaaaac aaattggctc cagctgatac aatggaagat gaacttcacg
    50941 atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca gaaaaagatt
    51001 ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca ctttctatct
    51061 ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta gttttgggat
    51121 ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct atcggtcgca
    51181 ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac caattagcca
    51241 cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa ttaaaaaaat
    51301 ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact actacaagtc
    51361 ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat actttgacaa
    51421 aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact ttattcaatc
    51481 cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc gatcaacatg
    51541 agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt cctatagggg
    51601 taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa ttagcgtccg
    51661 tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat ccatttttct
    51721 taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat aaaagaatcc
    51781 aagtagatat tgctacagat gcttctagtg tggttatttt cactgcaaat ttcggcacag
    51841 aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca tttgagactc
    51901 aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta aaagccggat
    51961 cagtatttga aacagtaaca gaattcaaaa tcaaaacaag aaaggaataa ctcatcatgg
    52021 aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa aaaactacac
    52081 aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac aatggaggcc
    52141 atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct cgagacgata
    52201 ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt tttactttag
    52261 atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga atggtcctta
    52321 aattaaaaga agctggacac tcaatcgatc atgggtttga gttattagtg gaaggaacaa
    52381 tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta ggtgttgtat
    52441 tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca ggtaaaaaag
    52501 tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc atcggcttcg
    52561 gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa atggttccag
    52621 tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg gaattggcag
    52681 attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt ttgcaaaaaa
    52741 ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca actattgacc
    52801 aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa aatcagcgga
    52861 ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt caactgttaa
    52921 atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa ttagatacac
    52981 tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca ggagctggat
    53041 ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc aaaaacaatg
    53101 tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt gcacacattg
    53161 gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa aatcaacgat
    53221 ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg tgagcctttt
    53281 gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt gttctaattg
    53341 tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt atgaaaaaga
    53401 accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg tagagtgcgt
    53461 tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc cgtccgattc
    53521 gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa aaaaagctta
    53581 cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa gtgatttata
    53641 tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca attctatcta
    53701 taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa gcttccttga
    53761 tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg ctaaactgat
    53821 ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg gcgataaccc
    53881 gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa tgtttgctga
    53941 atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca ctgacttttg
    54001 gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt ctgtttatga
    54061 tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg gtactcagct
    54121 ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata caccaaaatc
    54181 aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag aagaaatcga
    54241 gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat ctgcagcaaa
    54301 aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc caaatggcaa
    54361 gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag atctagcaga
    54421 tgctttattg accattttcc accagcatta ccactaccag gatattcggc gtgtaggcgt
    54481 gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg aagatcctga
    54541 aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa aaaaatacgg
    54601 ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca tcgcacgtag
    54661 ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa aagatgagac
    54721 ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt ggattaaaat
    54781 gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaatg aagaagaagc
    54841 acgcaaacag cctcttccta aacaacaaat gacattgcaa gaaatcgata gtatactgac
    54901 agaagccttt ttatctcgta aagcagtaat cattcagctc aatttaaaag atgaatttgg
    54961 aagactcttg gatgaggtcg aagggcactt cattggtgaa gcctatgaag attactttat
    55021 gattgaccat cagccgatat tgtgggaaga cgtacggtat attgagttaa aggaaacaaa
    55081 aaaatggttt gtgacatgag aggttgtgac agaagtgatc aacttcaaaa aataagaaag
    55141 gatttttcga aaattgcttc ttaaattttt gtggaatttt ggcttatttt tcgaagaagc
    55201 aacttctgga acaacgtata ttagatatca gaggttgtga aaaagtcgct tagttttgag
    55261 taacaagcca aggcgctgat ttcacaacct ctgtctatct atccatctat tcggtaagat
    55321 tcatgcatga cgtcttcttt atttagtatg tagagccctt tttcttctga ccattcaaca
    55381 taaatcagct gttttacatc tgcatagcct tctttcttca agttctccat aagccatgct
    55441 tcagtcaatt gattggactg aagcacttta tgctgaattt gcccttcatc tacaagcagg
    55501 atcgataaag ctttgtcttc atctgatttt ttcaatacac tcatttgacc ggcattctct
    55561 aggataacat gtttggcatt ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc
    55621 aactgctcca tctcgatatg gttatttgtc aactctgtca tgttgatgac gccgtctttg
    55681 attagaacag atggctcgcc tttgacccaa cgattgacct tttcattttt ttggacgata
    55741 cgttcgatac cataaatcat tactgcccaa agcgcaatgg caaataggac atgccctaca
    55801 tggacattgt catcataggt tgattcttct aagatcccac ctaaaacaag cgtatagact
    55861 aaatcaaacg gtgtgatttc tgacatggat ttcttcccaa gcaaacgtac cactaataat
    55921 aaaccgatca acccgaacac cagtttcatt gtaattgaaa aaatcatttt ttctccccct
    55981 aactatctgt taattaaatt atatatgctt tctcacgaac acgcatggaa tatacaagga
    56041 agaaatgtat ccttttacta aaaaaaggac tgtgacactg tagtgacccc caaaagttag
    56101 acttttttgg agtggagaaa tccactcctt ttttttatgc tctaaaatga tatttttaca
    56161 caaccgttac ggttgtttta cgaaattgga cg
//
LOCUS       DAFEZB010000007        54446 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFEZB010000007
VERSION     DAFEZB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.007"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            10..588
                     /locus_tag="LP859_000497"
     CDS             10..588
                     /locus_tag="LP859_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028477.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LP859_000498"
     tRNA            complement(828..911)
                     /locus_tag="LP859_000498"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LP859_000499"
     CDS             966..1145
                     /locus_tag="LP859_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028478.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LP859_000500"
     CDS             1294..2511
                     /locus_tag="LP859_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3028479.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LP859_000501"
     CDS             2695..3531
                     /locus_tag="LP859_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028480.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LP859_000502"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LP859_000502"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL3028481.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4977..6035
                     /locus_tag="LP859_000503"
     CDS             4977..6035
                     /locus_tag="LP859_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL3028482.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6039..7073
                     /locus_tag="LP859_000504"
     CDS             6039..7073
                     /locus_tag="LP859_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL3028483.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7175..7891)
                     /locus_tag="LP859_000505"
     CDS             complement(7175..7891)
                     /locus_tag="LP859_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL3028484.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7860..8645)
                     /locus_tag="LP859_000506"
     CDS             complement(7860..8645)
                     /locus_tag="LP859_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL3028485.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8636..9385)
                     /locus_tag="LP859_000507"
     CDS             complement(8636..9385)
                     /locus_tag="LP859_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL3028486.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9683..10207
                     /locus_tag="LP859_000508"
     CDS             9683..10207
                     /locus_tag="LP859_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL3028487.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10223..11722
                     /locus_tag="LP859_000509"
     CDS             10223..11722
                     /locus_tag="LP859_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL3028488.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11810..12412)
                     /locus_tag="LP859_000510"
     CDS             complement(11810..12412)
                     /locus_tag="LP859_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3028489.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12516..13472
                     /locus_tag="LP859_000511"
     CDS             12516..13472
                     /locus_tag="LP859_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL3028490.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13777..14733)
                     /locus_tag="LP859_000512"
     CDS             complement(13777..14733)
                     /locus_tag="LP859_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3028491.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14907..15467
                     /locus_tag="LP859_000513"
     CDS             14907..15467
                     /locus_tag="LP859_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028492.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15460..16284
                     /locus_tag="LP859_000514"
     CDS             15460..16284
                     /locus_tag="LP859_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL3028493.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16294..16911
                     /locus_tag="LP859_000515"
     CDS             16294..16911
                     /locus_tag="LP859_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL3028494.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16929..17612
                     /locus_tag="LP859_000516"
     CDS             16929..17612
                     /locus_tag="LP859_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL3028495.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18295..19578
                     /locus_tag="LP859_000517"
     CDS             18295..19578
                     /locus_tag="LP859_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028496.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19594..20481
                     /locus_tag="LP859_000518"
     CDS             19594..20481
                     /locus_tag="LP859_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028497.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20498..21334
                     /locus_tag="LP859_000519"
     CDS             20498..21334
                     /locus_tag="LP859_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3028498.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21337..21960
                     /locus_tag="LP859_000520"
     CDS             21337..21960
                     /locus_tag="LP859_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028499.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21957..23248
                     /locus_tag="LP859_000521"
                     /pseudo
     CDS             21957..23248
                     /locus_tag="LP859_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LP859_000522"
     CDS             23820..24401
                     /locus_tag="LP859_000522"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL3028500.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LP859_000523"
     CDS             24404..25711
                     /locus_tag="LP859_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL3028501.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LP859_000524"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LP859_000524"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL3028502.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LP859_000525"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LP859_000525"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL3028503.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LP859_000526"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LP859_000526"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL3028504.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LP859_000527"
     CDS             complement(29220..29597)
                     /locus_tag="LP859_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028505.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LP859_000528"
     CDS             29853..30581
                     /locus_tag="LP859_000528"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL3028506.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LP859_000529"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LP859_000529"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL3028507.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LP859_000530"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LP859_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL3028508.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LP859_000531"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LP859_000531"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL3028509.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LP859_000532"
     CDS             33716..35155
                     /locus_tag="LP859_000532"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL3028510.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LP859_000533"
     CDS             35178..36230
                     /locus_tag="LP859_000533"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL3028511.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LP859_000534"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LP859_000534"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL3028512.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LP859_000535"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LP859_000535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL3028513.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LP859_000536"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LP859_000536"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL3028514.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LP859_000537"
     CDS             39749..40663
                     /locus_tag="LP859_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3028515.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="LP859_000538"
     CDS             complement(40796..42361)
                     /locus_tag="LP859_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL3028516.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="LP859_000539"
     CDS             complement(42354..43805)
                     /locus_tag="LP859_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3028517.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="LP859_000540"
     CDS             complement(43819..44835)
                     /locus_tag="LP859_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3028518.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="LP859_000541"
     CDS             45055..46143
                     /locus_tag="LP859_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL3028519.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="LP859_000542"
     CDS             46197..47723
                     /locus_tag="LP859_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL3028520.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="LP859_000543"
     CDS             47725..48930
                     /locus_tag="LP859_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028521.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="LP859_000544"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="LP859_000544"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL3028522.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="LP859_000545"
     CDS             50428..51852
                     /locus_tag="LP859_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL3028523.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="LP859_000546"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="LP859_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL3028524.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="LP859_000547"
     CDS             53425..54402
                     /locus_tag="LP859_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL3028525.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17403 a         9316 c        11919 g        15808 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaattgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt tattgtgaat aaatgaacat aattaggagg agaaagatga
     4981 acttatttga catttttcga tcgactttga cagatatgtc gattgtcagt gcaatcactt
     5041 caacagtttt tattatatta ttagggtttg tttgtagaaa aaaggggatt ttttctgaat
     5101 ctgttggaaa aattctatcg aaagtcgtct taacagtggc tttgccagca ctggcatttc
     5161 gagctttcat gcaagatatc aatacagaat tactgatgca aggaatcaat gtcttgattt
     5221 ggggattagt tatttatatt attcttattc tagtttcaaa accttttttc ttaagctata
     5281 aaggggataa gcaaacaacg ttgcgtgtcc tgacgatctt tggttctacg acctttttcg
     5341 gaacgcctat cgtcagtgcg atttatggtc ctgtcggcgt gatgtattcc tcaattttca
     5401 atattggcta ccgaatcttt ttgtattctt atggatacat aaaaatgagt ggcttgaaga
     5461 tggaagctaa aaatatcaaa gcgatgttcg tcaatccaat cattatcgct acttttgccg
     5521 gccttttcat ttggctgttc caagggtatc tgccacaagt gtcagttaca ggtgcagaag
     5581 gagagataag ccaagtagcg tttttgcgaa ttgaccagac tgcaccgtgg ctatttcagc
     5641 cgatggatta tttagctggt ttaagttcac cattggcgtg gctagcaatc ggcgcaacat
     5701 taggtgaagt aagcttcaaa aaagctgcat ctgaaaaaac ttcatggtat tacagtctag
     5761 tcaaagtggt atttgtacct gcattgaata tcgtgattct agcagttttg acggcaacga
     5821 atattctacc agtcaatttt gaagcactgg caacaatcgt tatcatgatg gctacaccag
     5881 ctgcaacagt ggcagcagcc tatgctatca gttttgatag ggaggcactt ttagcctcca
     5941 atgcatcctt actgtcaact atttttgctg tggtactgac acctgtatgg atcgttgtat
     6001 tacaaatgat tcatggcata ggtttatttt cataagaaat ggaggaagaa agtatgagaa
     6061 tcgcatgtta cggtgttcgt ccgaacgaga tagatttttt tgaaaaattg aatatttacc
     6121 attatgatct cagtctttat gaagaactgt tgacacatga caatatcgtg tcagcaaagg
     6181 accatgatgc tgtattgttg aggggaaatt gtatagcaga tgagaccaat cttgcaaaaa
     6241 tgcaggaata tgggattcgt tatgttttta cacggactgt gggagtcaat catatcgatc
     6301 tacaagcggc agctgatttt ggtatgacag tagcccgtgt accatcgtat tcgccaaatg
     6361 cgatagcaga gttatctttg acttttgcca tgatgctttt gagaaatact gcgtatacga
     6421 cgatccgaac atctttcaaa gattttcgag tggatgagca aatgtttagt cgtgaaatca
     6481 gaaattgcac agtagggatc ataggaacag gcaggatagg gttgacggaa gctgttttgt
     6541 ttaagggact aggagccaaa gtgatcggct acgatattca tcagacagaa gctgctaaaa
     6601 aagtattgac gttccactcg cttgaagagt tattagccaa aagcgatatt gtcagtattc
     6661 atgtacctca tatcccaggt gaaaacgatc aaatgatcaa tgaaggattt ttagctcaaa
     6721 tgaaaaaagg tagcatcttg attaatacag cccgtggaga gcttcaagat aatgaagcta
     6781 ttttaagagc tttgaaaagc aatcatttag ctggttttgc aaccgatgtt tttgcaaatg
     6841 aaacagaggt ttttttccga tccttcaagc catgggagac gattccagac cctgctatcc
     6901 agcaattagt agaaatgtac cctagagttc ttgtaacccc gcatgtaggt tcaaatacag
     6961 atgaggcatt atcaaatatg atttcgacaa gtttcgagaa ctttcgtgaa ataatagaaa
     7021 ctggaaaaac gaaaaatgag gtatctcttc ctaaagcccg tcaattaaga taagaaaaca
     7081 tggatattcg tcaataaaat gtttaaaaat agtccaagta aacagttgtt acttctgtat
     7141 acttggacta ttttgtatgg gataattcga tgactcaaca gtaatctgtg agctttttcc
     7201 gcccattctt tgtcaattca taacaggtta gcggccggcc gatttccaaa tatttgattc
     7261 tttcgttcaa tatatcttgt tctactaaaa aattcaaata tttcttgacg gatatacgtg
     7321 acagtgagct tttttgagcc agttcttttg ttgaaaaaat agtatcttct tttagaataa
     7381 atgcacttat tttttttaaa gtcagttttg acagaccttt aggcaaatcg atgaatgatg
     7441 gtatttcgtg aatgggagtg gtgtggttga atacttgatc gatcaatttc tgatcggctt
     7501 tttcagtcgt ttggattgtc tgagagaaag caagatactt ttcgatcgcc agctgaaaac
     7561 gttgcaaagt gaacggtttg atcaaataat ctaaggcacc gtagcggatc acattcttta
     7621 tggtttcgat atcatctgca gcagtgatca aaatcactgg gatatcattt ccttgctgtc
     7681 taagccaaga taaaaaatgg ataccacttt cgccaggcat gtatacatca agcagcagca
     7741 gatctgcgtt tttttgtctg aaatattttt ttccttcttc tacatttctg aaactttcga
     7801 ttacgttcat attagggatg ctttccacaa aagtacgatt caattctgca accattggat
     7861 catcttcgat tatcaggaca tttgtcatca ttttgatctt ccttttttgg taattctaga
     7921 tagacgattg taccgccatt tgtttgtttg gaaaattcta aaaatccacc gtggcgtttg
     7981 acgattgaat ggatgagatt caatccaaac ccacgattga ttcccttcgt cgaaaaacct
     8041 agcgtaaata ttttttctga gatttcttct gatatcccga caccgtcatc gcagacggtc
     8101 acgatgaata cttcagcttc tgtgtcatag tttaaatgaa tccggacctg tccgtttctt
     8161 tcatgttttt gtagcacggc ttcttttgca ttatcaagta agttccctaa cacttgcaaa
     8221 agtgaatgga tggagtcatc catctccatt ttagggataa aagaatcgct ttctaacagc
     8281 atttcaacag attgttcttt ggcttcgttt attttgccta ataaaaaacc ggcaattgca
     8341 ggtactttga tcagacttga gacttggcca atctcttttt tataattttg atttagctgc
     8401 tggataaaat gggtgacttc ttcatacttc ttcaaatcaa tcaaaccaga aatgacatga
     8461 agtttattca tgaattcatg ggtttgagct ctcatggagt ttatatattg tttcgtcccc
     8521 ccaagctcct ccattaattt cattacttct gatcgatttc gtaaagttgc gactgctcct
     8581 aaaaaacggt cattgctaaa aagaggtgta acgctagaaa tagtttctat cccatttaaa
     8641 tacatggatt gagtccgaac ggtcgtttga tgctcaatcg tctctttaaa caaaacgtca
     8701 tatagattat cactgatttt tttcctactt cgaaatccaa tagctgctct gtttggtaga
     8761 aaatgttttt tgcaggtgta ttgatcaaga ggatagtttt atcagaagag atggctaaga
     8821 ttccttcatc tacttcattt tcgataatcg ttttttcact taactgttct gctatttctt
     8881 gtggttcaag tccaagcagt acttttttca ttttttgggc aagaaaaacg gcgcctatga
     8941 tgcctacgct aagtccaata atcaatgaaa aaacaatcat tttttgtcca tctttgactt
     9001 gattgtcaat cgtgttgttt gttaagccaa cacaaactgc accaataatc tcttgatcat
     9061 aataaatagg gacgaaatag cggaagccga accctaacat tccttctttt tcagagaaaa
     9121 attcttgtcc tccaatcact tttttcacat cttcatgact tgagaaagga agaccgatgt
     9181 aacttgggtc tgcgtgagaa tatcgaatgc cttcagggtc catgacaacg ataaaatcta
     9241 aattcgatgc aatcatgatt tcatttgtta ttccttgaat cgcatgtgtc gggtcaccag
     9301 tctgcagctt tttttggatg agtttatcat tggcaacgat tttagcaatg ctagagattt
     9361 tatctgacac actagatcgg ttcatcgaaa gaataaaatt cctgatcaat atggcagaaa
     9421 caatcaaaga aacgaatgtg gagaaacaaa cgacaaggat gatcgtgttc tggagcttta
     9481 atggtttttt cttaaaaaaa ggaaacatag gctacctcga aagaatttat tgtgaaaatt
     9541 ttaacatgaa aacaaaaatt taacaaattg tcaacgataa acacaacctt cctataaaaa
     9601 acaccactga gtcggtaaaa gaagctatac agaattttca aacattggta tgatagacgt
     9661 agaaatggaa ggaggcgtgt atatgatagg gggaatcgca cttgagacga tttattggta
     9721 tacattgctg atttgtgcag gattggcagt cctattgatt atttttggag atgtattcga
     9781 ttttgatggc ccgcttgatc cgatgcttat tatcccttgg ttaactttca caagcttatt
     9841 tggttttcta ggtgagcgtt taacagatca atcatcttta tttttattta ttgctagtgg
     9901 aggcatatcg atagtgttgg tatttttgct taatttctat atattgatgc cgatgaaaca
     9961 tgccgaatca acgatgtcgt cttctgaaaa aactctggaa ggacaagtag ctgttgtgat
    10021 cacaccgatc cctgtacgag gtatgggaga aattcagttg aaaagtgtta ccggttccat
    10081 tagtcgtcca gcttgttttt acagccccca agaacagaca gctgagcggg gaacgaaagt
    10141 attgatcatt gagatcaagg aaagaatctg ttatgtcatg ccttatgaaa gcatgataaa
    10201 agcttaaagg agcgtgtgtt aaatggaaat accagttgtt tttatcgcga tcgcaatcgc
    10261 agctttgatt ttactgatgt tgctgatcgt ctttgtagcg aagtaccaga cagcaaaacc
    10321 ggatgaagcg ttgatcatca gtggaagtta tttaggaagc aaaaatgtgc acattgatga
    10381 aagcaataac aaaatcaaaa ttgtgcgtgg aggtggcgca ttcgtcttgc cagtttttca
    10441 acgttcgaat cggatcagtc tattatcgag taaattggac gtctccaccc ctgaagtgta
    10501 tacggaacaa ggcgtaccgg tgatgtgtga tggtacttcg atcatcaaaa tcggttcatc
    10561 tgtagaagag attgctacag cagcagaaca gtttttaggc aagacaagag aagaactgga
    10621 aaatgaagca agagaagtat tagaaggaca cttgcgctcg attcttggtt ctatgactgt
    10681 agaagaaatc tatcaaaatc gcgataagtt cagtcaaagc gtacaagaag tcgcttctgt
    10741 agaccttgca aaaatggggt tgatcatcgt ttctttcact atcaaagagg tcagagacaa
    10801 aaacggttat cttgactctt tagggaaacc aagaatcgcc caagtcaaaa gagatgcaga
    10861 tatagcagaa gcggaagctc taaaagaaac gcgtatcaaa aaagctcaag cagaaaaaga
    10921 atcacaagca gctgaattac agcgacaaac agagatcgca gaatcattaa aagaaaaaga
    10981 actgaaatta gcaacctaca aacaagaaca agatgttgct aaagcaaaag cagaccaagc
    11041 ctacaattta gaaagtgccc gagcacaaca acaagtaatc gaacaggaaa tgcagattaa
    11101 aatcgtagaa cgtcaaaaac aaatcgaact ggaagaaaaa gaaatcacac gtcgtgaaaa
    11161 gcaatatgat tcggaagtga agaaaaaagc agatgccgat cggtacgcta aagaacaaga
    11221 agcacaagct caaaaagtca aagaagtgac tgaagccgaa gcagaacgat tccgtgtgga
    11281 agcactggca gaagcggaag ccaataagac acgtttagct ggacaagcag aagccgaagc
    11341 agcactagca aaaggaaaag cagaagccga agcaaaacaa aagatcgcca atgccttcaa
    11401 ggaatatggc gaagcagctg tcttgagtat ggttatcgat atgctaccgc aattgatgcg
    11461 cgaagcagca cagccgctag gaaatatcga aaaaatctct gttgtcgata caggaagcag
    11521 ttcaggagaa accagtggag cgaaccgagt gacaaattat gccacaaatt tgctttcttc
    11581 tactcaagaa acactaaaag cgacaacagg acttgacttg aaggaaatcg tagagaattt
    11641 gtcgacacgt gggactacta gccaagtaaa tatgtatgaa gcgccaaaag aggcagaaca
    11701 aaactcgatg aaggaagaat aaagtagtac acttcaccgt tcaatcaaaa atggaggaag
    11761 actttttaaa gttcttctct ccattttttt attttattgc aaaagaaaat tagtcctcgc
    11821 tatttttctt ttgtttttgg atcttagctt ctatctcttg taaatttgtt cttagcaatc
    11881 gttctttctt ttggagttcg accaaatctt ctgatagttc ttccttcttt tctgtttcag
    11941 gaggacgaag aaagaaattg ataccgattg caatgaatgt ccctataaaa gtatctagta
    12001 cacgttggat agcatacaaa gcggattcgc cttgagggat gctcaacgca atcagcaaca
    12061 acgtagctat tgcggaaatg ataccggaat tattgttaat accatctgac acgacgatca
    12121 caataataac tagaatagga agcaagatca attctaccaa aaaatcattg tgaaaatagc
    12181 ttttgacaaa aaaataaatg atagctaatg taccacctaa tgtattgccg atgatcctag
    12241 actttccaaa agaaacggtt gtagtcaagt cctgacgcaa agaaaaaaca gcagataatg
    12301 cagcaatcat cgggattcct cgttgagtca aatgaaaaaa caagatacat aaaaaaacag
    12361 ctaaggcact tttgatcgta cgcataccta gtcgaaaacg gccaaattgc atagagattc
    12421 tcctttttgg tctttttact atcataccta aacttttgtc tgatagctag tttttcatta
    12481 ttcaagtata attaaaataa aaaaggggga agtaaatgcg tataggaatg cgaacagtca
    12541 aaacagtgat cagtgtcttc ttttcgatgg tcacagcggg tagcttatcg ctgttgtatt
    12601 ggccagctgc agggatcatt gcattattaa gtgtaggaaa taccaagcgc tctacattgc
    12661 gtacgggcat gaatcgaatc gcagccttta tattagctac ggcaacagca ctgttcagtt
    12721 tttttgctgt tgggtatact atttttggct tcagtctttt tttattcctg ttcatcccgc
    12781 ttgcctctcg cttcaaatta accgaaggga tagtagtaaa ttcggttttg gtcactcatt
    12841 atttagtgga ggaaaacatg tcttggcagc tgatcggcaa tgaagcggga ttaatggcga
    12901 ttggattagt atttgctttg cttgcaaatg tttacatgcc agatcgtacg aagcagctga
    12961 aggaaaacca aatacagatc gaaaaagaat ttcgaaatat tttgagacaa atggcagaat
    13021 ttcttttatc agaaaataaa gaagatgtgc agatcaaatg cgaacattta ctgacattca
    13081 ttcgcgaaag ccaagaagat gccagagaac atcaagaaaa ctactggctc agacagccgc
    13141 tatactacga aacttacttc tctatgagaa gagcacaggc caatgtgatt aaagacatgc
    13201 tggaaaatct agagagaatc cagcaaccag cttattatgg aaaacatatc tatggtttat
    13261 tgatttatac tgcagaaaca ttttcagagt caaatgatgg cagacaaatt ttaacacgta
    13321 ttgaagaagt ttatgtattg tatcggcaaa tgcctctgcc gactagtcgc cctgaattcg
    13381 aggatcgagc agaattgttc cagtttttgc agtcttttaa aagttttatt gaaatcaagg
    13441 cagaattctc ccaacaaaaa atccaaaaat agaggttgtg aattcagcta atattcaggt
    13501 caaaaagctg atttcacaac ctcttcttta aacggtgttc tcctgtcaac tcctcggtca
    13561 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgctcc ttgtgactcg
    13621 gagctgaacg gtaggctcac aacctctttc ttaaacggtg ttcaaaagct ttcctctgcg
    13681 gtaataagtc cagccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta
    13741 cttgatgcag aacagctttt tcacaacctt ttaaaattac atatttttcc atagaacttg
    13801 catttgatcc aagatttcga tgacatcttt tgtcaagaag agagagcgat tgggcaagtc
    13861 accttcaatc atgtgtacca tattctttat ttcgtaattc attgcttcag atgtattccc
    13921 gctttcgatg aattcctttg taccatctgt aaataagatc tccgcacgat cagcacgcgg
    13981 gtaatcagca atcgtcacat aaccgttctc aaaggcaacg ataccgactt ttggcatttt
    14041 tgcctgaaaa gtcaagctga cagcagcaag ttcattttct ttgttacgca agatagtgac
    14101 ggattgttcg tccacgcctg tttcaaaagg aaccattgta gaggcaacta cttcaggttg
    14161 agagctcaaa aagaaacgag cgaaagatac agcatacgta ccgatatcca acagagctcc
    14221 tccagctaat tctggattga agaaccggtt tttagggtct ggttctttgt aactgccgaa
    14281 aggtgcttgg atcattttta aagcccccag ttttcctgta tccattattg agcgtaactg
    14341 ctgataaagt ggcatgttga agattgtcat cgcttctgct aagatcaaat tcttttcttc
    14401 tgctaagcgc atagcgtcag ccagttcttt tttattcatc gtgatggctt tttcacatag
    14461 aacgtgtttg cctgcttcca atgctgccaa gatatggtcg atatgctgtc tatttggtac
    14521 agcaatgtac acgatgtcga tttcagcatc gtctaacatt tcttgatagc ttccgtaagc
    14581 ttttggaata ttgtaacggt gagcgaagtt ttccgctttt tctgaagttc tggaagcaac
    14641 tgctgttagt tcgctcgtct cttgattgaa gctttcggca aactcatgtg cgatggtacc
    14701 aagaccaacg attccccatt gatatttttt catctgaaaa cgctccttca tagttggatt
    14761 gagtttcttg tctgtctatt attataacaa aaatcacgaa gaataaagag gagaggactt
    14821 gaaacattaa aaaaaatgaa agcagcgatt taagaggtgc tttttgatca aaacttaagt
    14881 agaatagtta aaaggagggg ataaacgtgc atcaagtata tattgatctt attatgagtg
    14941 tattattaga acagtttgaa actgaaaaaa attttttaga tgaatacttg ctgatcagtc
    15001 cagaacaatg gcaaaaatgg aaagatggcg agaaaaatct gccatctgaa gcgatgcaga
    15061 aagtaaaaaa tcttttcact gattatgaat ggatgctaac gcaaaagatt ttgcgtcaaa
    15121 cgattatttt tcccgaaaaa cgaaacgttg ccgttgcaga gtacaagcaa atcaaaacac
    15181 gcatcgcgca aaaatggctt agcagcaaag cagcaaaagt cgagctgatc cctttgaaaa
    15241 ataatcagca ggctggtaga taccttgact tgaaagtaag tattcaatat gatgaatggg
    15301 gctatgatga tattttgaat tttcgtctgc cagcagatgt tcaaaaacag atcgaaggag
    15361 aaaaaatcga acttctcgat tgggtgaatg aaaatctgga agaaacttac gttcatgagg
    15421 cgaatgggga acgtgaagaa caacgaaagg atttttcaag tgaataaatt tttaggattt
    15481 ggagcagttg tgattatgat cgcagcaatg ggctatgtta tttttagcga tgatgattcg
    15541 catgttcaag ccggcaatca agcagtcgaa cgaacaagca ccaaaacaac tgctagtaaa
    15601 gaaaagaaca gtcaaacaac cagcagcaag gtaaaggaca aagaaacaaa agatcgtacg
    15661 tccaaacaat tgccagatgt taaaacagac gattggaagc tagttttggt tggtccagat
    15721 cacaaattag aaaaagaaat tgatgagtct actcaattgg catcattaag taacggatac
    15781 ttgatcgaca aacgcattgc tccagaatat gaagcatttg ccaaggctgc tgaagcagct
    15841 ggatttccat tagtgatggt ctcagcttac cggtctgtat catcccaaca acaagttttc
    15901 gaacaaaatg tccaagatgt gatgagtcgt caaggtgtat cagaagagga ggcgacaaag
    15961 attactaaag aaacgattac ggagccagga tacagcgaac accacacagg tctggcagtc
    16021 gatgtcgtcg atcaaaactg gtacaacagt tatccttcta ctgtattgga tgcaagttac
    16081 ggagatcagc ctggagctaa atggatcgca gataatgctg cgcagtatgg atttatcgtt
    16141 cgttatccaa aaggacacga agatataact aagatcacat atgaaccttg gcacttacgt
    16201 tatgtcggta aagaaaacgc tgagtatatc accaaacacc aattgacgct agaagaatat
    16261 ctgaatgaat taaatgaaaa gtaggaattg atcatggcga aaaaaaagtg gaaaaagcaa
    16321 aagcgcaaga atatccgttg gccagcagtg gttgccggtg tagcagtgat actcttagcc
    16381 tttgtttttt ccttgaagaa tctgtcttct ccttttactg ataatcagca agaaaatgaa
    16441 gcgcaatccc atcagcaatt tattgaacgg cttgttccgc atgcccaaga actacaagat
    16501 ggttatggag tgcttcctag tatcatttta ggacaagcta tattagaatc aaattgggga
    16561 aaaagtacct tggctagcaa atacaataat cttttcggga tcaaggctta tggggatcag
    16621 aagaaagtaa gcctggaaac aaaggaattt gttaacgaag aatggataac tatccaagga
    16681 gacttcaaag tgtatgattc atgggaacag tccatggatg accatacaca actgttcgtc
    16741 caaggagtag attggaatcc tgcgctgtat gaaaaagtga ttacggccac caactatcag
    16801 gaagccgcac aagcattgca agatgcaggt tatgcgacag accccggtta tgcgcaaaaa
    16861 atcatccagg tcatcgagac gtatcaattg aatcagtatg accggagata gcaaaggaga
    16921 agtggctgat ggcagaacaa tttacgccag aaatcatgac ggaattgaga aaagatatcg
    16981 taagagtacc ggaaatcatt ttagaagcaa gcgggatcaa gatatttgga aaattgatcc
    17041 gttcgatcat ctttactacg gatattgcga tcatacgtaa tacaaatgcg aatgcagtaa
    17101 ttgctgtgta tccttttaca cctcatccag cgattactaa aagcattatt gaggcagctg
    17161 acattccagt tttctccggt gtaggtggtg gtttgacaca aggtgctcgt tcttcctaca
    17221 tgagcatgtt cgcagaagcg caaggttcgt taggggtcgt tttgaacggt cctacgcctt
    17281 tagagacagt aaaagaagtt tgtcgagtag ttgatattcc agttatcagt acggtaacgt
    17341 cgaaatatac agaaatagat gaaaagttag cagctggtat ttcgattatc aatatcagtg
    17401 caggtaaaga aactgcagaa gcagtacgcc attttcgtaa aaaatatcct gaacttccta
    17461 tcattgcgac aggtggtcca acggaagaaa gtattgctga gaccattcaa gcaggagcaa
    17521 atgccatcac atatacgcca ccaagtagcg gagagctttt tagtcgtaaa atggaaaaat
    17581 atcgggaaac tgagaaaaag aatcatgaat aacaggtgag aatcaaaaga cgaggaagag
    17641 cagatagtgg ctcttcctcg tcttttgtac tccagtacta tttgttcaga attcaaaaaa
    17701 aagagaaatt tttttgtttt tttacctgta atgtacataa gaagagttaa aaccttttgt
    17761 tattattagc attctaacaa aaaaatgtct tgatttattg aagcgctttc tatagaatga
    17821 aaaacgaata aaaactaaaa aaggcacccg acaatttagt agcagctaaa ttgtcggggc
    17881 ttgaaaagtc agcgggaact gaccaatcaa gttgccatag tcaatgcttg tgcatcgacg
    17941 tgtatattgt acctaatttc atacagaatt actagttatt tttctgtttt ttagggaaga
    18001 gcgcacacga agaagcatgc ttatttagct atgtcaagga tattgggcag ttgtctgata
    18061 tccttttttc tttgcgtaat tatcctccca tcattgaagc aagaagtgtt tttagcgatg
    18121 aaagaagaat cagcactatc taatttttct tgaaagtatt gagttgttca tcctaggcag
    18181 aacgataagg caagtcttgg tttttattcc ccttgaaaaa gatcgtttaa taaatatgtt
    18241 ctgtataaaa atacagtata ttgaaaaata acaatggaag tagaggtgca gagaatgaag
    18301 aaaagaatga gaatcatctt agctttaagt gttatgttat tcttcttgac ggcttgtggg
    18361 caagaagatg acaaaaaaat cacgctgaac atggcttggt ggggttcgca agtccgacac
    18421 gatgcaacag taaaagtaat cgagctttat gaagaaaaaa atccccatgt gacgatcgat
    18481 tatgaatttt atgattatga aggatattac acaaaattag gtacactagc agcatcagat
    18541 gatatctggg acattttcca actgaacaat caatttcctc aatacatcaa tcagattgaa
    18601 ccattagatg aatatgtgga gcaagggata attgatattt ctaatgcaga tgaatcctat
    18661 ttagcaacga cgacttacga cgggcaatta gttggactgt ctaacggggt gaattcgttt
    18721 gcgattgctt acaataaaaa tatatttgaa caattaaata tccctgaacc agcttcaaat
    18781 tggacatggg atgaatttga acaaatcagc tatcaaatca ctgaagaatt aggaatctat
    18841 gctatttcaa gattcgaaga ttttatagct ggctgtatta ttcaaatacc acaagttgag
    18901 aaaggattga atttttataa tcgggaagat ctatctaaca gcttgggatt tgaaaatcca
    18961 gactatctaa cagatttttt ccagctgcgt aaaaatctgg tagacaaagg ggcccatcca
    19021 gaaccaggtg gtggagctgc aagtacgacg gatctgtctt ttcaggcggt tagggataga
    19081 gaagcggcaa tggtgtttct atcaagtaac cagttgacag aaatattgtc aggggctcct
    19141 gaagatatgg agattcagtt gattaatcca cctcgcagaa aagcagatgg agaatctggt
    19201 atccctttac gttcttcgca aatgctgagt atggcaaaaa gctcagccca taaagaagaa
    19261 gctgctaagt ttattgattt ctttcaaaac agtgaagaag ccaatgaaat tttaaaagga
    19321 gaacgtggtg taccaatcat gtcgaatatt cgagaacagc tcgctcgtgc agatgatgca
    19381 actttgcaag cgacatttga ttatttagaa atgatttccg atatggacaa cggcgaagtg
    19441 aatgtctttg atagtgcttt gaatccggaa attcaagacc aatacaatct tttagttgaa
    19501 aaagtcattt atgaagaagt aacagcagaa gaagcagcag agagtcttta tgagtttgcg
    19561 tcaaacattc taggatagca gcaggaggaa ggcatgaaaa aagaaaaact aaaacggttc
    19621 ttggatcaag acaatgtggt cggttacgta tttgcttctc cattcatcct aggatttcta
    19681 ttgataacag tgataccgat gatcttgtca ctttattatt cgttcaccga ttatcgtttg
    19741 ggaaatccag tctcatggat cggcttggat aattatataa agatgctgag tgatgcccgt
    19801 tttttgaact ccatccgagt cacttttctc tatgtatttg catctgtacc tgtgaaactg
    19861 atctttggtc tattgattgc ttatctttta acgaggaata tcaaaggaac gactttcttt
    19921 cgttctttgt attatgtgcc tagtttgatc ggtggcagta tttctatcgc tctagtttgg
    19981 cgggagttgt tttctaaaaa cgggttagtg aatattttcc tggaacgatt aggcgcacct
    20041 gcgctttcct ggtttagcga tcaaaaactg gtcatgatcc ctcttgtttt gatgtctgct
    20101 tggcaattcg gttcggcaat gatcatcttt gctgcaggta tcaaagagat cccaggctcg
    20161 tattatgaag cggcagagat cgatggagcg aacaaatggc agacgtttta caagatcacg
    20221 ttacctcttc tttcgccgat cattttgtat aacttggtta tgcagacaat cgcggcgttt
    20281 atgactttta cgcaggcatt tatcattacc caaggtggtc cgaatgatgc cacgaacttt
    20341 tatgctcttt atgtgtataa ccaagctttt tcttttaatt ctatggggta tgctagtgct
    20401 atttcgtgga tcatgctgtt gattatggga gctgtcacct ttctgatttt caaatcatca
    20461 aaaaaatggg tcgttgtgtg aatgagagga ggaagaaatg aataatacag taacgaaatg
    20521 gtttcaatac ggttttattt tgctattagg tctagtcatg atttatccgg tcttatggat
    20581 gatcgcaggt tcttttaaaa cggatcaaga gattatgagt ggttcgctga atttgattcc
    20641 agagactttt caatgggaaa attacgcaaa aggatgggcg gggtttgctg gaatttcatt
    20701 ttttactttc tttaggaatt cgtttgtcat ctctgcagtt tcgacgatag ggacagttct
    20761 gagctcgact tgtattgctt acgcattatc gagaatcgat ttcaaaggaa aaagattctg
    20821 gttcgtcgtc atgttagtaa cgatgatgat tcctgcacaa gtgattttga ttccgcagtt
    20881 tattatttac aatcggctga atttggttgg gacgtatgtg ccgctgattt taccacattt
    20941 ttttggacaa gcatttttca tttaccagat catgcaattc atggtcagca tccccaaaga
    21001 gttagatgaa tcagcgatta ttgacggatg cagcaagtat agtgtcttta ccaaaattat
    21061 ttttcctttg ctgaaacctt cgatcatcac gacgatcatc atccagtttt attggaaatg
    21121 ggacgacttc atgggaccat tgatttattt aaacaagccg agatcttaca cggtatcgat
    21181 tgccatcaaa ttatttgcag acgctggatc tagtacgact tacgcgagta tgtttgcgat
    21241 gtctacgttg tcgctgctac cggtttttct gattttctta ttcttcaata aatatcttgt
    21301 ccaggggatc agtacaagcg gtctgaaggg ctgaatatga aaaagccaat agaagagatg
    21361 tttctttaca agctcaacta ctatgtctgt cggatttttt taaccagtct tatcagtaat
    21421 gctcttttcg ctttagttct aggatgtttg ttcactttag gattagtgct gtctgctcgt
    21481 gttgtctatc tgatttattt tttgatgatg tatccttctt attatctatc tgtccattac
    21541 ttggcagaaa aaaaggtatg ttggaggaaa ggaaaaatca aacggatgtg tttcatcatt
    21601 acgttgtttc agctgtttgt cattattttt gcagttgatt ggtatttatt agtgggaaag
    21661 attggagctt ggtatctgat tccttttcta ttgtgtctgt tgctttttgt atcccatagt
    21721 ttttttagta cgctaattga tatctgcaaa gaagataaag cagatagtct tgtttatttg
    21781 atcaaaagga atatctggct gttgtcaaaa aacgtcaagg ggtttctgtg gtatttatgc
    21841 cttttgtatg gactgttctg gtgtatacag catgattttt tactcactgc attactgtat
    21901 ccagggctgc catttgtcac agtattttgc ttaaaagaaa aggagaatga ctttaaatga
    21961 agaaaaaata tgtacaagtc ggacttggtg ggagagcgcg ttttttttat gaggcaattg
    22021 cctctacgta tcatcaaact tgtgaactag tcggtttttg tgatgtatct caaacaagga
    22081 tggattatgc aaatcggata cttgtgaaca agtatggaca gaaaaaagta aagacatgta
    22141 tgccatcaca atttgagcaa ctactggatg acgtacggcc ggctgaagtc attattacgt
    22201 cagtagatcg aacgcatcat gattatatca ttcgtgcaat gaaaaaagga tatgatgtta
    22261 tttgcgaaaa acctctgact atcgatgagg aaaaagctca agaaataata acggtccaaa
    22321 aacagacggg aagaagcctg cgagtcactt ttaattatcg ttatgcaccg tatcacacga
    22381 agataaagga actgatagaa agtgatgtga ttggtgatgt gttttctatc cacttcgaat
    22441 ggctgttaaa tacgcaacac ggggcagatt atttcagaag atgcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt atagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa taagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaatt
//
LOCUS       DAFEZB010000008        53370 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFEZB010000008
VERSION     DAFEZB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.008"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(437..600)
                     /locus_tag="LP859_000548"
                     /pseudo
     CDS             complement(437..600)
                     /locus_tag="LP859_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(953..1642)
                     /locus_tag="LP859_000549"
     CDS             complement(953..1642)
                     /locus_tag="LP859_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL3028526.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="LP859_000550"
     CDS             complement(1705..2469)
                     /locus_tag="LP859_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL3028527.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="LP859_000551"
     CDS             complement(2497..3195)
                     /locus_tag="LP859_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL3028528.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="LP859_000552"
     CDS             complement(3207..3986)
                     /locus_tag="LP859_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL3028529.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4002..4946)
                     /locus_tag="LP859_000553"
     CDS             complement(4002..4946)
                     /locus_tag="LP859_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3028530.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4992..5771)
                     /locus_tag="LP859_000554"
     CDS             complement(4992..5771)
                     /locus_tag="LP859_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028531.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(6156..8231)
                     /locus_tag="LP859_000555"
     CDS             complement(6156..8231)
                     /locus_tag="LP859_000555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL3028532.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8233..9150)
                     /gene="glyQ"
                     /locus_tag="LP859_000556"
     CDS             complement(8233..9150)
                     /gene="glyQ"
                     /locus_tag="LP859_000556"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL3028533.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9536..10348)
                     /gene="recO"
                     /locus_tag="LP859_000557"
     CDS             complement(9536..10348)
                     /gene="recO"
                     /locus_tag="LP859_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL3028534.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(10491..11390)
                     /gene="era"
                     /locus_tag="LP859_000558"
     CDS             complement(10491..11390)
                     /gene="era"
                     /locus_tag="LP859_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL3028535.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11405..11800)
                     /locus_tag="LP859_000559"
     CDS             complement(11405..11800)
                     /locus_tag="LP859_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL3028536.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11784..12260)
                     /gene="ybeY"
                     /locus_tag="LP859_000560"
     CDS             complement(11784..12260)
                     /gene="ybeY"
                     /locus_tag="LP859_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL3028537.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12289..14481)
                     /locus_tag="LP859_000561"
     CDS             complement(12289..14481)
                     /locus_tag="LP859_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL3028538.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14505..15476)
                     /locus_tag="LP859_000562"
     CDS             complement(14505..15476)
                     /locus_tag="LP859_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL3028539.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16182..17561
                     /gene="brnQ"
                     /locus_tag="LP859_000563"
     CDS             16182..17561
                     /gene="brnQ"
                     /locus_tag="LP859_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL3028540.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17717..18163)
                     /locus_tag="LP859_000564"
     CDS             complement(17717..18163)
                     /locus_tag="LP859_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL3028541.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18190..18366)
                     /locus_tag="LP859_000565"
     CDS             complement(18190..18366)
                     /locus_tag="LP859_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL3028542.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18528..18956)
                     /locus_tag="LP859_000566"
     CDS             complement(18528..18956)
                     /locus_tag="LP859_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL3028543.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            19048..19908
                     /locus_tag="LP859_000567"
     CDS             19048..19908
                     /locus_tag="LP859_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL3028544.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(20003..20353)
                     /locus_tag="LP859_000568"
     CDS             complement(20003..20353)
                     /locus_tag="LP859_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL3028545.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(20369..22096)
                     /locus_tag="LP859_000569"
     CDS             complement(20369..22096)
                     /locus_tag="LP859_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL3028546.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(22310..23236)
                     /gene="trxB"
                     /locus_tag="LP859_000570"
     CDS             complement(22310..23236)
                     /gene="trxB"
                     /locus_tag="LP859_000570"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL3028547.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23381..24817)
                     /locus_tag="LP859_000571"
     CDS             complement(23381..24817)
                     /locus_tag="LP859_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL3028548.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24814..26217)
                     /locus_tag="LP859_000572"
     CDS             complement(24814..26217)
                     /locus_tag="LP859_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL3028549.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            26447..27349
                     /locus_tag="LP859_000573"
     CDS             26447..27349
                     /locus_tag="LP859_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3028550.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(27390..29276)
                     /locus_tag="LP859_000574"
     CDS             complement(27390..29276)
                     /locus_tag="LP859_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3028551.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(29372..30223)
                     /locus_tag="LP859_000575"
     CDS             complement(29372..30223)
                     /locus_tag="LP859_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3028552.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30680..32464)
                     /locus_tag="LP859_000576"
     CDS             complement(30680..32464)
                     /locus_tag="LP859_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028553.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEKYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(32464..34215)
                     /locus_tag="LP859_000577"
     CDS             complement(32464..34215)
                     /locus_tag="LP859_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028554.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(34357..34581)
                     /locus_tag="LP859_000578"
     CDS             complement(34357..34581)
                     /locus_tag="LP859_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL3028555.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34832..36418
                     /locus_tag="LP859_000579"
     CDS             34832..36418
                     /locus_tag="LP859_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL3028556.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(36728..38503)
                     /locus_tag="LP859_000580"
     CDS             complement(36728..38503)
                     /locus_tag="LP859_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL3028557.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(38714..39616)
                     /locus_tag="LP859_000581"
     CDS             complement(38714..39616)
                     /locus_tag="LP859_000581"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL3028558.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            39895..42240
                     /locus_tag="LP859_000582"
     CDS             39895..42240
                     /locus_tag="LP859_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL3028559.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(42403..42669)
                     /locus_tag="LP859_000583"
     CDS             complement(42403..42669)
                     /locus_tag="LP859_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028560.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(42662..42985)
                     /locus_tag="LP859_000584"
     CDS             complement(42662..42985)
                     /locus_tag="LP859_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028561.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(42997..43203)
                     /locus_tag="LP859_000585"
     CDS             complement(42997..43203)
                     /locus_tag="LP859_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028562.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(43200..44255)
                     /locus_tag="LP859_000586"
     CDS             complement(43200..44255)
                     /locus_tag="LP859_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL3028563.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(44255..44650)
                     /locus_tag="LP859_000587"
     CDS             complement(44255..44650)
                     /locus_tag="LP859_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028564.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(44693..44947)
                     /locus_tag="LP859_000588"
     CDS             complement(44693..44947)
                     /locus_tag="LP859_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028565.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            complement(44944..45339)
                     /locus_tag="LP859_000589"
     CDS             complement(44944..45339)
                     /locus_tag="LP859_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028566.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            complement(45693..46694)
                     /gene="ccpA"
                     /locus_tag="LP859_000590"
     CDS             complement(45693..46694)
                     /gene="ccpA"
                     /locus_tag="LP859_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL3028567.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            46904..48007
                     /locus_tag="LP859_000591"
     CDS             46904..48007
                     /locus_tag="LP859_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL3028568.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(48069..48671)
                     /locus_tag="LP859_000592"
     CDS             complement(48069..48671)
                     /locus_tag="LP859_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL3028569.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(48674..49114)
                     /locus_tag="LP859_000593"
     CDS             complement(48674..49114)
                     /locus_tag="LP859_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL3028570.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(49240..50178)
                     /gene="galU"
                     /locus_tag="LP859_000594"
     CDS             complement(49240..50178)
                     /gene="galU"
                     /locus_tag="LP859_000594"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL3028571.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(50193..51218)
                     /locus_tag="LP859_000595"
     CDS             complement(50193..51218)
                     /locus_tag="LP859_000595"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL3028572.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(51262..52092)
                     /locus_tag="LP859_000596"
     CDS             complement(51262..52092)
                     /locus_tag="LP859_000596"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL3028573.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(52107..53045)
                     /locus_tag="LP859_000597"
     CDS             complement(52107..53045)
                     /locus_tag="LP859_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL3028574.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(53182..>53370)
                     /locus_tag="LP859_000598"
     CDS             complement(53182..>53370)
                     /locus_tag="LP859_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3028575.1"
                     /translation="NSNGILRRNGLPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT        14870 a        11316 c         9454 g        17730 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     4081 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4141 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4201 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     4261 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     4321 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     4381 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4441 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4501 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4561 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4621 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4681 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4741 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4801 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4861 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4921 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4981 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     5041 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     5101 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     5161 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     5221 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     5281 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     5341 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     5401 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5461 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5521 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5581 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5641 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5701 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5761 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5821 tacataaaaa tgacagtttt ttatttaaaa aaacataatt aaactaatat attcacagaa
     5881 atatttgtaa acaagttttc cctatataat cataccacaa actgctttct tgactaggga
     5941 aatttacgat ttaaaagtaa ataaaacatt aataagataa caattaatcg attttacttt
     6001 tatttcctta attttcatag gtgtttttaa aagttacaca aaaaataaaa taacaaaaaa
     6061 aattaacatt gaattaataa tagttattta taacaaataa accagagaaa tagcctattt
     6121 tgtgcctgtt ctctggttta tttacatatt agaaattatt ttgtgatgat ttgatctaaa
     6181 cttgctagtg acaaagccat ggcagcaatt tgacttaatt gcgttaatcg gttcttacgt
     6241 actgctggtt cttctgccat gaccatcgtc tgatcaaaat aattttcgat cagtgggcga
     6301 aggcttacaa gctgatcata attttcgttg atcgtttggt cagcaaagct tgttttcatt
     6361 gcctctactg ctttgtgcag ttctttctcc gcttcgtttt cgaacagttc agggtctact
     6421 tctgattgtt tttcaagctc cgcttttttc gctaagttca cgacacgtgt caaagcttcc
     6481 atcgatggtt tgaagtcttc atctgcaaga tgctgcttga acaattgtgc tgctgcaaat
     6541 acttttgtca gatctttttg ttctgcattc aaaacagctt cgatgacatc atgacgaata
     6601 ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac ctgttgttgt
     6661 cccgctgaca agccaatgcc aaaacgatcc gcatctttac tgataacttc actaatcgtt
     6721 tcttgcagaa cagataaagg aaaggcccat tttttcgatt cgatgattcg aacgacacca
     6781 tatgcttgac ggcgcaaagc atatggatcg tttgagccac ttgggatcat cccaactgca
     6841 aagaaagaaa agatactatc taatttatca gcaatcgcta aaacagaacc tactgtagtc
     6901 tcaggtagat ctccttcact ggatataggc atgtaatgtt cacggattgc tgttgctacg
     6961 gctggtttct cgcctttaag cagagcatat ttttctccca tgatcccttg tagttcaggg
     7021 aattctccta ccatgttggt tactagatca aatttataga tttcagcagc gcgttgcaga
     7081 tccatcaatt cttcctcaga tagtcctact tgctcgccta tgatgcgggc gatgacttgt
     7141 acccgttgca ttttctcgta gatgctaccg attttttcat ggtaagtaac agtttttaat
     7201 ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct
     7261 tctaaacgag cagtcaatac tttttgattg cctcgaacca cattttccaa ttttacccga
     7321 tcaccattac gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg
     7381 aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat
     7441 ttttcttcgt agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct
     7501 agtaaatctt cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt
     7561 gtaacgatca tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt
     7621 tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga
     7681 gaggctctgc ctgttttgac atccaagatt tcgaatggga ttacttcatc atctaacaat
     7741 gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt
     7801 acagggaaag tcaggctttt aatgacatct gctaaacctg tcaatacttc tttggattcc
     7861 ttaccatgag tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg
     7921 atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt
     7981 ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct
     8041 tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg
     8101 atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt
     8161 ttcatgcttg gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag
     8221 tcttttgcca tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa
     8281 caaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat
     8341 gttgcggata cgggaaagat aaccggcacg ctctgttacg gatactgcac cgcgagcatc
     8401 taataaattg aatgtatggc tgcatttcaa aatataatca tacgctggat gaacaagatt
     8461 ttcatcgata cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc
     8521 ttgatcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc
     8581 gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata
     8641 agaagcaaga cgttctagtc cataagtgat ctcagaagtg actggtttac aaggcagtcc
     8701 accaacttgc tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca
     8761 acccacaccg gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc
     8821 gtgttctaat ggatcgattc ctaaaaggcg caagctttct aaataaagct cttggatatt
     8881 ctcaggtgaa ggtttcatca cgacttggaa ttgatgatgc tgatacaaac ggtttgggtt
     8941 ttcaccataa cgtccatcag ctggtcggcg agatggttct acgtaagcag cattccatgg
     9001 ttctggtccg attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt
     9061 gtcatacgct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat
     9121 catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt
     9181 gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac
     9241 atagggacga ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt
     9301 tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac
     9361 tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt
     9421 cttgtttcta taatagatcc tctcactgaa cgatctattt gatctacggt taataataga
     9481 cggaactccc tgtgtttgtc aaatggtttt gattttttgt tgtatgtttc atttactatt
     9541 cttctttttt aggtagatct ttttcagaca tgtctttctc tggcgtcttt aatacgtgtg
     9601 cccaagactt catttgatcg atgaactttt tgcttttcaa atgaataccg acatattcat
     9661 catacagctg gtcgatgagt tcacggattg cttgtttggt ttcttctttc agatggattc
     9721 cactgatttt atcataagaa atagctgaaa acatccgaac gaaataaaca gctcgaggat
     9781 ctgcatgata acggtgatcg tccatcgacc aatgtctttc gcatatcaca ccaccatatt
     9841 tggaagaata gtcaaatttt ccttttgttt ccccacatac gacgcagtgt gtccagattg
     9901 gtgcaattcc gaagcgttgc agtatttgaa cttcaaagat attcgtgatg atttctgcat
     9961 catctccttg atccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat
    10021 attgatgatc ttcgattgct gcatctacta gatttagcaa ataggtacca taaccgctca
    10081 aaaagatatc ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg
    10141 acaaaccttc acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg
    10201 gagctaatgg ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag
    10261 attccgtaaa gattttgact aatttatctt tttctttgta attacggcta gataaaatca
    10321 agcctttcga ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag
    10381 cagattctgc cgatccagat tatttatgtt tcgatcttcc gccgtttttc tcaacgtgcg
    10441 gatttttatc cagtttattt ctatacttaa cctataagaa gttcagaata tcagtattca
    10501 tcttttcggt agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact
    10561 ttgacccaaa gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct
    10621 ttcgttccta cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta
    10681 tctctttcga cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc
    10741 gaatcaacaa cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc
    10801 tcacggatca attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga
    10861 aaatattgtg ggccctctgg catttcactg actaatacat ccatcaaacg ttcgacgttg
    10921 tttccttctg tagcagaaat tggaatcact tgtgcaaagg gcatctgcgt tgaataatct
    10981 tcgatgattc ccaacaactc gtctggatgg attttatcga ttttattgat aatcagataa
    11041 acaggagaat gcacgttttt tagacgttca ataataaaat cgtctccccg gccgcgtttt
    11101 tgatcagcgc tgatcataaa taagaccgcg tccacttctt tcaacgcact gtaagctgct
    11161 tctaccataa aatctcctaa acgatgcttt ggtttatgga ttcctggtgt gtcaataaaa
    11221 atcaactgag cttctggtgt agtatatact ccttggattt tatttctagt tgtctgcgcc
    11281 ttatcactca tgatggcaat tttttgaccc acgattcgat tcaataatgt ggattttcct
    11341 acattcgggc ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc
    11401 ctttttaatt aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaacgatga
    11461 cggcaaaaca agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa
    11521 tcggatgaaa atgaaaatcc gtaaacatgt ctacgacatt ttcaaaaaca gtattgatga
    11581 tctctactat ccatacgaga aaaacagcta aaagcagcca taaccattct atccgatcaa
    11641 gttggaaaat cacgcccata aacaaagcga ggattccgaa cgcaacatgt ttcctcatgt
    11701 tgcgttcttc ctcaaaaaca gttttgactc cttggacggc aaattccacg gacatcagga
    11761 aatgtttgtt tttctctacg tttttatctt ttaagtccat aggcatctaa gatctctttc
    11821 tgtaaaccaa acatttcttt ctcatcctct ggtgtcatat ggtcataccc gttgatatgc
    11881 aagaatccat gaaccgctaa aaagccaagt tcccgatcat acgaatggcc gtaagcttct
    11941 gcttgctctg cagcacgttc agtagagatc atgatgtctc ccagtacacg aggcaatgga
    12001 ttctctccat cttcaaaaat cactgggatt tcgtcttcac cctcttcttc caaggcaaaa
    12061 ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggatcgct
    12121 gcgttgtcca tgaaagtaac agacatttct gtttcttctg gcaactctaa atatgatgct
    12181 gcaaattgca acaagtcgtc gatttctttc attttctctt ctgagacttg gtttgtttca
    12241 tccatgaagg taatatccat tatacatcgc ttctttctat tcttcttgct attcttcttg
    12301 ttcttctttt atcttctcaa cttccgattt cattttagga tatttgatcc gagaatggaa
    12361 ggtactgctt aaaccgctga tcaatgattt ttctacttta cggatttctt ttaacgtcaa
    12421 tccactgtca tccaattgtc catcggaaat acgctcttct ataagattat ggacaaaact
    12481 ttcgattttt tcgcttgttg gatggtccat tgcccgtact gccgcttcac aactgtcagc
    12541 tatattgacg attcctgctt cacggctttg aggttttggt ccaggataac ggaattgctc
    12601 ttccgtgact tccgggttgc gttcctttgc cttaacgtag aaataacgca tcagcgtggt
    12661 tccatgatgc tgtctgcaaa tatcgatcac catttgcggc atattataat cttccaatat
    12721 tttggcacca tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa
    12781 attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg aaaaagctgg cgtgtttgat
    12841 cttgccgata tcatgataat agcaagctac tcgagttagt aaagaacgtc caccgatttc
    12901 tgctacagca ttagcactta gattggctac catcatgcta tgatgatacg tacctggtgc
    12961 ttcttctaac aactgtttca ataaagggtg attaggattg ctgagttcat ttaatacgat
    13021 cacactatca tcattgacca tcaattcaat ataggggtgc agacctatcg tcagcaagaa
    13081 tgagaacaat gcacctgtga atccgcagac tagcgttaac caagtcgttg catccccaaa
    13141 gctcattcct tggtagataa tcaaaatgac atccatcatc acagggaaaa taatgaccca
    13201 catagcagcg aataaccctt gttctgagat ccgtttgcgt ctgacgatcg ttgccatgag
    13261 accagaaaat acataggaca tcaaaataac tgtcagaaag ttcgtgccaa tcgaatcata
    13321 aaaaatgaaa agtgcggata ctgcttggaa aataccagcc atgatccctg ctcttcgatt
    13381 caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg taaaacagcg gaatataagc
    13441 agcttgttct gtttggaaga gttggaaaaa tttcattagt actacgctga ctgacattgc
    13501 tgtcacgtag aaaagtacgt attttgttct ttctcctgca ttttgaaatt gaagtgaatt
    13561 ataaatcagt acaccgattt gaagcaaaac agctaaaacc atggcaatga gtgggaaaat
    13621 cgaagtcgtt tgattcgtca atcctagtaa ttccaatttt ttcatcgctg aagcatcaat
    13681 ttgatttcct tcgcgaacaa tgatttctcc ttggaagatc atcactggtt gtacagcttc
    13741 tcgtgcagat tgttttaatt cttctgtctt tttctcattc aatacatcat tgactacaat
    13801 gccttgatcc actaagtagc ggatcatttg ttgctgctct gttgtgacat tcaaatactg
    13861 aatctgatct tctgcttctt gtttaaaagc ttccaattcg ctttcacgaa cttgtttact
    13921 catctgttcg tcaatgagct tcaaactttc atccctaacc gtgtgaattt ccgtttcagt
    13981 catttcaaat atcgtcgaat aaaaattgtt cggcagtttt tggtaaaacg caacgttttc
    14041 tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc cgctcttctg tagtaggttt
    14101 cgggatattt tcatcttcct tcgccttatc taccttgtca ttataagatt tgttgatggt
    14161 atcattcgtc ttatcgatca attcgaataa ttgcttgatc cgattgttct ggtcatctgc
    14221 cagatctttt tgataagtat attctggagt cactgcttct gctgctagtt ttcttctttg
    14281 ttcggtttct tccgtgttct cgatcgtttt attcgcacgg atactctctt ctgctacttg
    14341 gccttctcta aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcaaacaat
    14401 gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt ccgagttttt cttgtagttt
    14461 ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga
    14521 tccgcctttt cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt
    14581 gcttcaaaat taacaaaatc gatttttttg atttgcttta gtgttcgttc tgcatgaaca
    14641 agaccacttg tcgtccctcg aggcaagtct atctgacttg tatccccatt gacaatcatt
    14701 tttgattgat aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt
    14761 gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaatggt
    14821 gcgatttcga ttacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg
    14881 tataaagcat cataaacagg ccgcagataa ggatccactt tttctttcag atcccctggt
    14941 agaaagccta ggttttcccc tgcctctaca gcgggccgcg tcaaaatgat tttttgcact
    15001 tcgccttttt ttaaggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg
    15061 gctggcccta caccaaatac gatgtcatgt ttggctaccg attcaacata tttcttttga
    15121 ccgctgtttt ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac
    15181 atctcaacaa aataatctaa tgtacctttt tgccccattt tcaatgccgt cacaacatcg
    15241 ggcgtactta cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca
    15301 gccaaggaaa tctgggattg ttcaccaatg atctgaatga tttctccacg tgtatggatg
    15361 gttgtttccg ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg
    15421 ctgacatcat cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat
    15481 agacatcccc tttttttcga ttcattcttt cctattttac catactcaga agaaactata
    15541 tagcaaataa gaaaaggttg taaaagagtc gtttaaccat acgtaccaag aaacgttaaa
    15601 aaatagccgt ttccattcgc taaaggtctc gttttgataa aaagaatctg agaatgtaag
    15661 ttatatgaaa gtggagaaca aaatattccg tttcattcag caggtattct ctaggtattt
    15721 ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca
    15781 ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta
    15841 ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg
    15901 aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg
    15961 ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa
    16021 ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat
    16081 tttaaggtgg tggttttcat ggaatctgat attaatggca ttactaattg ctgcaacgtt
    16141 tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt
    16201 attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag
    16261 gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cacaaactgg gtcagtgctg
    16321 gcgtaggatt tgccatctca ggcgctcttt tcccattact agctatctta gctgtagtag
    16381 tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct
    16441 tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca cggacagctg
    16501 ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat tcattaggta
    16561 tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc catcccacac
    16621 ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcatta ttagcaatca
    16681 tttttgtcgt ttctttattc cacccaatgg gaaatctaaa tcaatctgct gcagatactt
    16741 accaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt gatgcagtag
    16801 ctttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt ttcaaagata
    16861 aaaaagtcac agaattaaca gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga
    16921 tctattttgg acttgttctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa
    16981 atggcggaac tgcactttct cagattacta atttttattt aggtaacttc ggcgctagct
    17041 tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt
    17101 ttgcacaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa
    17161 cgactttcgt ctcttttgtc gttgcaaatg ctggtttgga caatatcatc caatggtctt
    17221 taccagtatt gatgcttttg tacccattgt cgcttgcgtt gatcttgctt tccttaacag
    17281 caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt gctgctgttc
    17341 ccgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa agagttgtag
    17401 caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga cttggctgga
    17461 tggtcccaac gttacttggt tacgctggca gcttactctt ctattacgcc tatcgcctat
    17521 cagggtacaa acaagaagca aatgaactac cagaagaata accagatcaa acacaattta
    17581 acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat tctttcgtct
    17641 agctattttt tagatttctt ttttacgagc acaaaaaaac tgcggacaat gattgtccgc
    17701 agtttaaatg ataaaattaa gattgtaata gttctttcac aatggcattt acttggtttc
    17761 catctgcttt tcctttgact ttaggcatga tcgcacccat gactttgcca aattctttca
    17821 ttgatgaagc accagtttgt gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg
    17881 aaagttgttc cggcatgtat ttttcgacta tcacaatttc actttttact ttttcagcta
    17941 aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcgc
    18001 gagacaacac cgttaattct tcttcttctg tcaagtcgtg accttcttta atctgctcgt
    18061 tttgtataga tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca
    18121 tcgctgtttt catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc
    18181 atcacagaac tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac
    18241 acttggtttt tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac
    18301 ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt
    18361 tgacatgtta attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata
    18421 tctcaaaaaa caagactgtc ctgtaaacat tatatctaat gctgttttga ccgtcaagga
    18481 aatcttgctt ttttttaaaa ttatcaaatg ttattttatt taaatcctta ttgacaattt
    18541 tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg tacatccacc gagctgttct
    18601 tcgaaataat tcattggaca catatggatt tctttcgttg ttccgcacac cgtacagata
    18661 aagtgatggt gatggccgat ttccccttga cagcaatgaa aacgaaactt catctctcca
    18721 tttaaatcgg tttcttccag taaatcgatt tctgcaaaat catggagatt tcgataaatc
    18781 gtatcgtaac ttactccttt gaattcctga ttcatatagt caaaaacttc tttagcggat
    18841 acgtagcgat tgcgcttcac taaataagtt aacaatgctt ctcttttttt agtatattta
    18901 aaaccgttat ttttcaatgt ttctagtgat tgttcgatca atgtctgttt tttcatgact
    18961 tattcctcca aatcaaaatg attacgaagt acacattcta gagtatggta taataaacat
    19021 aaaaaaaatg caagtgaggt tttatccatg tcttccgatt ctacagaaat cgttactttt
    19081 tttgtcatct ccgactctgc tggagaaact gcgacaaaat tagcgcaagc cactatggcc
    19141 caatatccta gcgttgagtt caatttattc cgcagaacat ttgtgacaga tgagaaaaca
    19201 ttgatgcagg cactgcaaga tgcactagaa gaaaaagctc ttgtcttaca cacattgatc
    19261 aatcaagaat tgatcgagat gacgcgttct ttttgtgaag aacatcaatt atttagtttt
    19321 gatgtgatga ctccgccgat atcagaaatc gagcgcttaa ctggtatcaa gcccattcgg
    19381 caacctggcg cccttcattt attgaatgag aactatttca aacggattaa agcaatggaa
    19441 tttgctgtaa aatacgatga cgggaaagat cctcgtgggt ttctagaagc agatgtcgtt
    19501 ttgctaggcg tatcccgaac ttccaaaacg cctttgagct tgtttttagc taacaaaaat
    19561 ttaaaagttg ccaatcttcc tttgatccca caagcacata ttcccaaaca gctttgggag
    19621 attgatccta aaaaaatcgt gggattgacg aataacccag atattttaaa caatatccgt
    19681 aaagaacgga tgcgctctta cggattaaat cctgatactg cttattctga tatcgaaaaa
    19741 atacgagctg aactcgattt tgcaaatgat ctttatgaaa aactgggctg cgtagtaatc
    19801 aatgtcgctt ctttatctat tgaagaaaca gcttcgctta ttttgaatgc tttagaccta
    19861 gaagatcata gttattatgg aactgaaacc accgaagaaa cagactaaca gtttttcttg
    19921 tctttttctt aaaaaaaact ggactgtgtc agcgaagaag attcgttgac acagtccagt
    19981 ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagtgtc tggttcaaaa
    20041 tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact
    20101 tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt
    20161 ccactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt
    20221 tgagacgagt ggggtcactc aacattttga atagttggct gactttctct acttttgttt
    20281 tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta
    20341 ccactttttc catcagtctc aagttttttt attcttctgt catcttttgg ataatcgctt
    20401 cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatgtaaa
    20461 atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg
    20521 tatacttcaa tacatcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg
    20581 ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgttg
    20641 gtgcttcttc acggaatttt tccatcaaag cagagatttt cgcagaacct tcttgtccgc
    20701 tcattgtcac agaaatcgtt ttttctgcgt agtagccata ggtttcaaaa atcgtttgca
    20761 agccgtcata caacgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga
    20821 gcaatgcttg gatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt
    20881 cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag
    20941 caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg
    21001 ctgtaggtaa ttcgctagaa acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc
    21061 ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag tgctccgatt tggtttccgg
    21121 tcaatacttc ataggagcca tttggcaaac gaacggctgc acctaaacgg tctgcatcag
    21181 gatctgttgc aatcagcaaa tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa
    21241 aagcagagtg ttcttctgga tttggtgatt tgactgtagt gaaatctggg tctgcgacag
    21301 cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca
    21361 acatttttcc tgttccgtga agtggtgtgt acacaagttt caaatctttc cccattgttt
    21421 ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt
    21481 cttctccgat gatcgtgatc aatccgctat gttcagcttc ttcgtcagat aatacttcaa
    21541 cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag
    21601 gcatttgtcc cccatcttcg ccgtagactt tatatccgtt gtaagctgct ggattgtggg
    21661 atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg
    21721 gtgttggacg tagactttcg aatacataag atgggatatt gtgatgtgct aatgttttgg
    21781 ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc
    21841 ggcgttttgt ttcttcgcct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc
    21901 ttaccgtata gatgttcatt cgattgatcc caggccctaa gattccccgc atgcctgcag
    21961 taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat
    22021 catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca
    22081 cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag
    22141 cattcactta ttagatataa aagtatgagg accaaaaaac gcttaaatca agaaaggatt
    22201 cttttatcaa gcacggttgc aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa
    22261 aaaaaagagt aaaagaacaa ttgaaaccgt tcttccactc tttttaaact tatgcagttg
    22321 tttcttctaa agattccaag tatttataca cttgttgtcc agcgatccca ccatcaccaa
    22381 cagcagtagt gatttgtcgc aaatcttttt cacgaacatc tccgatggca aaaacgcctg
    22441 gtatattcgt cttcatttcg ttgtctgttg gaatccagcc agcttcgttc gtcaaccctg
    22501 cttctctaaa tggtgcagtt aatggatcca gtccaacata gatgaatact ccgtctgctg
    22561 ccaaatcaaa ctcattgccg gttttgacat tacgtgcttt cacacctgtc actaccattt
    22621 cattcccaac gatttcgtca acgactgtat cccacataaa ggaaattttt tcattcgcaa
    22681 atgcacggtc ttggatgatc ttttgtgcac gtaattcatc tctacggtgg acgatgacta
    22741 cttcagaagc aaattgtgtc aaatagatgg cttcttctac tgctgagtct cctcctccga
    22801 tcactactag acgtttgtta cggaaaaacg caccatcaca aactgcacag taagaaacgc
    22861 cacgtccagc aaattcttcc tcgccaggta cgcctaattt acggtggata cagcctgttg
    22921 cgataatgac tgtttttcct tcgtaagttt tatcttcaca aataacttct ttgtggctgc
    22981 cgtgatcctt gatatcttgc acgattccat aagcattttc tgtcccaaat cttgaaacac
    23041 cttcatacat cttataagct agatcaggac ccatgattga atcgaaacca ggatagtttt
    23101 cgatttctgc cgtattgttc atttggccgc caggggcgcc ttgttcgatc atcaatacgg
    23161 ataagtttga acgtgaagca tataaagcag cagtcatccc agcaggacct gcgccaataa
    23221 cgattacatc atacatatgt agattctcct ttacttaatc aagtaagcat actttacagc
    23281 caatgcgaaa aaaagtctat tgttctgctt gcgtttctcg aatagcttta ttatactcga
    23341 aaaatccgct gaaaaacaat agacttttat aaatcattga tcaatcattc gaaagatttt
    23401 ctccatttga atcaattact tttttatacc agttgaatga tttcttcttc gaacgggcta
    23461 atgtgccatt tccctcgttg tcacgatcga cataaatgaa tccgtacctt ttcttcatct
    23521 cacctgtacc agctgatact aaatcaatac atccccaagt cgtatagcct aatagatcga
    23581 ctccatctaa ttctactgca tctttcattg cttggatatg tgctgccaga tagtcgattc
    23641 gataatcatc ttccacataa ccattctcat cgggcgtatc gactgcacct aagccgtttt
    23701 ctacaataaa tagtggtttt tgataacgat cataaatatc attcatcgtt attctcaagc
    23761 ctaatggatc aatttgccaa ccccattcac ttgcttccag atatggattt ttgacagaag
    23821 caaagatatt tcctgcagtc tgttccaaga gttcgttgtc agtagtcgaa actcgagaag
    23881 aataataaga aaaggagata aaatcaacag tatgttcttt cagcaatgcg gcatccccag
    23941 gttccatttc gatctgaatg ccatttcgtt ccatttcttt caatgcataa gcgggatatt
    24001 ctcctcgtga ctggacatca atgaagaaat aattttctcg atctgatttt ctagaagccc
    24061 aaacatcttc aggattgcaa gtgtatggat aatatgctcc tgctgccagc atacagccta
    24121 ctttgttctc cggatctact tcatgagcaa tcttcgttgc gatggcactt gcaaccaact
    24181 catgatgtgc cgcttgatat ttgatttgtt cttcattttc tccttcttca aaatacagcc
    24241 ctgcgcccat gaatggtgca tgaaggatca tattgatctc attgaatgtc aaccaatatt
    24301 tgacaagtcc tttatagcga ttgaagatga ctcggcacag attttcataa aaaccaacga
    24361 gttctctgga acgccacgca ccatattctt tgatcaaatg catcggacaa tcaaaatgtg
    24421 taatcgtcac aagcggctcg atcccgtact tttgacattc tttaaagaca tcctcataaa
    24481 atttcaaccc agattcattt ggctcttttt catcgcccat tggaaaaata cgactccaag
    24541 caatggacat ccgataagtc ttgaacccca tttctgcaaa taaagcaata tcttccttat
    24601 aatgatgata catatcgatc gcaatttttg ctggataaaa atgattttca tcaaattcaa
    24661 acattttctt ttttccagta atgacaggaa aacggtcttc tccgattggc acaacgtcta
    24721 cgttcgctaa cccacgtccg ccttcatcat aaccgccctc gcattgattg gctgctgtag
    24781 ctccgcccca taaaaaatct tttctgaatc ccatcatact tcctccatta catgtgtatt
    24841 gcttgataat tctcttgcca gttcatcttg gacttcgatt ccttctgctt ttaaccggct
    24901 gattgtttcc ttgaattgcg gtaaatactg ctgatgtgca ataaacaact catccattat
    24961 ccgttttgct gtctttcctg atcgtataag cggattgatc gtaaacgctt gcaaagctgt
    25021 tccataatca ccagtcacgg ctgcttcgat cgtcagcaat tccatattct tcattacttg
    25081 caaccagcct ctttcagcag caggaaggga accaaacgct acatttcgtg cgccttgtgc
    25141 gccaacatag gcagtgactt ctactacaca atctgccggc agatcaggaa cagctccatc
    25201 attctttgta gataccacga tctgtgtttc tttatttcca tagatcgaag caatcgtttc
    25261 acaggcagca tctgaataat aagcaccacc tcgtttttgc aattgttcag gtttatgatc
    25321 caattctgga tctttgtata attcaaacag ctctgcttct gtttctttca cttgctgcgc
    25381 tcttgtacca atcgtttgat attcttctaa agcatgttcc agcatttctt cttcacggta
    25441 gtaatagcgg tggtagccac aaggaatcat gttcatttgt tttaattgtt ctttgaaaaa
    25501 cgggatatca aagatatttt tcggcaatcc attgtcttca tcgtataaac gatcaatcaa
    25561 ttctgccgtg cggtcatttc ctttattatc tgctactttg tgccaatgga agtgatttag
    25621 accggcaaac ttgtagatca gctcttcttt tttcttgcca agcattgttg gttctgtcat
    25681 catcgccatt actggcacat tgcataatcc gatcacttta tcccatttgc catatctgat
    25741 aactgcttct gtcaccatcc cgcttgggtt cgcgaaattg atcagccaag catttggaca
    25801 taaacgcttc atgtcttcaa cgattgccaa aaccactgga acggtacgaa aagctttgaa
    25861 tatcccgcca gcaccatttg tttcttgccc aatcatccca taataactag ggatccgctc
    25921 atctttgatt cgtgcatcta attgaccgac acgaaactga gtcgtaacaa aatccgcatc
    25981 tttcaatgct tcttcccgat ccagagttgc atagattttg acttcatacg gagaagcatc
    26041 ccacatacgc tgagacatct gactgacgat ctcaagcttt tcttttcctt cttctacatc
    26101 tacgagccag atttctcgta tcggtaattc ctcatatctt ttgataaatc cttccatcaa
    26161 ctcgggggtg taactgctgc ctccaccaat cgtagcgatt ttgattcctt ttgccatgtt
    26221 ctcactcctt tactttttac acttttatta tcttttttgt aaacaaaaca aacaatagaa
    26281 attcgtttct ttccagtaac atttacaaaa gttagtgttt tcttctggat ttttgtaaat
    26341 atcttgtcaa aaaatgtctt tattaagcta tataacataa atccgtttac aaatcatttt
    26401 caaaaaagtt ataatttacg taaaaaaaca agaagaaggt atctacatgt tattacgtga
    26461 aaaaatcaaa gaaatctcct tttctccttc agaaaatgaa gtgattcagt ttctcttaag
    26521 cgattctcca gcacttggcg aaatgaccat ccaagagatt gctgagcgat gttatgtcca
    26581 cccgtccaca ctcatccgtg tagcaaaaaa aatgggtttt gatggctggg tcagttttcg
    26641 taatgctttt ttagctgaaa cagcctatct tgaagggaat tttcaagacg tagatgcgaa
    26701 tcttcctttt tccaccacag acggtattat gacgatcgca aataaaatgg cttctttaga
    26761 acagatgacg attcaagata cactttctct cattcatcat gaccaactgc aaaaagccaa
    26821 acaactaatg cttcaagcaa aaaaaatcgt gattttcgcc agtaactcga atactttgat
    26881 ttctcaagat ttcatgctga aaatgaagcg catcaaaatg gatgtcacga ttgtcaccac
    26941 ttttggtgaa tcgttctatg aagcgtacaa ttgcacgagt gatatgtgcg ggattctgat
    27001 ttcttatacg ggtgaaaaca atgtgatcct tcgtaccgca gaaatattga aacaaaatca
    27061 tgtgccaatt ttagctttga ccagtatcgg tgattcaacg ctatcttcaa tgagtacagc
    27121 cgctttgcga cttacgacac gcgaacgact gtattcgaaa attggtaatt ttacgatcaa
    27181 tacatcgatt tgttatcttc tagacgtgtt atatagttgt gtttttgcag aaaattatca
    27241 acagaattta gtccatctaa tttctgtagg acaaaaattt gataaacgcc ctatcagctc
    27301 agccatcatg aaagagcctt tcaattttga aaataaagaa gtggactaaa aagaataatt
    27361 aagcctagtc cattctttca cctaaccgtt caggtcagtg cggtgataag tgcatcgtct
    27421 ggcccgaccg tctccccttg attttccaaa atatccaaat aatccgtgta attggtgacg
    27481 atcacaggtg tttctaaact gtatccttcc tcttcgattt tcttcttgtc gaagcggagc
    27541 aacagatcgc cttgtttcac tctttctcct tgctgcacca agcttttaaa atattttcct
    27601 tctaactgaa cagtatccaa tccgacatga atcagcaatt ccagacctgc ttcagaaacc
    27661 attccgattg catgtttcgt cgggaacagg gtcatgactg ttccgtcaaa aggggcacgt
    27721 acttctcctt tttcagggta gatcaagatt cctcgaccta atgttccttg tgcaaaagct
    27781 gcatcctttg ctgcattcag tggagcgatt tgtccttgta tcggtgtttt tacaatttct
    27841 ttacctgatg ttttctgtct atcatctgtt tcatctgctt cctccttcca aaagaaaaaa
    27901 gtcagcgcaa aggataccac catcgcaatg gcgatcccaa tcaatacatt aatcacatct
    27961 gttaaattac catctggtct aatatagttg ggaattgtaa agatgcctag tccaccttga
    28021 ttccaagaag ttaaattcat taacccacta tacgcgcccc ctaacgcacc accgacacag
    28081 gtaaaaataa atattttttt acgtggcaat gtaaacccat aaatagctgg ctctgtaatc
    28141 cccacaatac cagaaacaat ggctggtatt gcaattgctt tgtcttttgg atttctaagc
    28201 ttaaagtaga gtgcagtaag tgctgcagtt tgtccaaaag acactactgc aacaggtgtc
    28261 atgatatttt gccagccatt cacagaaaat tgcatcaatc ccattggtac gattgcccaa
    28321 tggagaccaa acatcattag aatttgccag aagaagccta cgattgctcc ataaataact
    28381 ggcgaaagtg ctaaaagtgt ttcaaatcca gccattaaaa tatcagttgc gaatgtgaat
    28441 gctggtccaa cgagtaaaaa acctaagggc attgtaataa gaacggtgaa gaatggaacg
    28501 aagaaattcg cgatcatctc aggtactaac tttttaattg gtttctgaat ttggctagct
    28561 aacaacacaa taaagataat gggaatgaca gaagaagcgt agttacgcgc aatccaggga
    28621 atgccgaata ctgtttgata aataggtgct tcaaaaatag tgccagaaaa tatggtcgtc
    28681 agtggtgctt ctgctgcacc actcaaagct tccagcgaca agctaggatt cattaatgct
    28741 gccccaagca acatccctac gaattcattt actttaaatt tacgggcagc tgttaaaccg
    28801 ataataacag gcataaattg gaatacagaa tcaccgatat tatcaaacat aacaaatgtg
    28861 ccagaaccgc ggtcaaatgc gcctaagaag acaagtaatg ctgtcagtcc acgaatcatc
    28921 ccaccagcag ctagaatagc aaggaatggc tggaaacagc ccgatattcc atcaataagc
    28981 atatcaaacg gtctcatttt ttctgttgaa acagcctcat caaaagtaag gtttcctgct
    29041 cttataacct cttcaaacac gactggcaca tgattcccga tgacaacttg gtactgtcct
    29101 cccgctcgca ttaccgtcac aacaccgtcc atatttttca agatctcatc atttgctttt
    29161 tcttcgtttt ttaatttgaa acgaagcctt gtgatacaat gtgtcaaacc attgatattt
    29221 tccttcccgc cgacattttc tacaattttg ctcgctaatt cttgatattt tcccatttct
    29281 attccctcca atttattagt ggaaggttta gccaactgaa tgtcactacc cttatttcat
    29341 gcgttcttgt taatttatcc tcctttttta tttatttaca acgattctca tgcactaaac
    29401 gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaatatct
    29461 cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa
    29521 taacttggta caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc
    29581 gtgtcagaaa aaaacgtagg tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa
    29641 atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt gtagtaaaca
    29701 ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa
    29761 atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt
    29821 tttccccgat ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg
    29881 gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa
    29941 tatacactga atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac
    30001 aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttctgaaa
    30061 gtccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca
    30121 ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatctc
    30181 tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttac tcctttccat
    30241 gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat
    30301 gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct
    30361 ttatattatc gaaagcaagc gcttttgtca acgctttctt agtggctatt aggcacttct
    30421 gactagctgt cgaatctttt ttaaatctgg tgactacgac actaccgctc acagatgaag
    30481 aatctgggaa gctctgtttt attttcactc tagcgcttag gtcaaaatgc tactatgcta
    30541 ctgtcccaac ttctaatata cggtgttcca gtatcagctt atcggtgata agccaaattt
    30601 atgcaaaaat ctggaaagcc ttttttacaa tttttttctt atcacctaaa gctgaacgga
    30661 gccctgtcac aaccactttt tacacaccac tgctttgcag tttgtacata tctgcataca
    30721 aaccattttg cgctagaagt gtttcatgat tcccgcgttc tactatatga cctttgtcta
    30781 aaactaaaat caattctgca tcccgaatcg tggacagccg atgggcaatc gcaatcgttg
    30841 tccgtccttg tcgcattttt gtcaatcctt cttggatcaa tccttctgtt tcagtatcga
    30901 tatttgctgt cgcttcatcc aatactagta ttttaggatc tgtcacgatt gttcgcgcaa
    30961 aagtgatcag ctgtcgttgt ccgctagaat agctggcacc gtgttcgatc acttttgagt
    31021 gatatccttt tggtaattca tcgatgaact tgtttgcttg aacaaactca gctgcatggc
    31081 gaatctgttc gtctgtgata gttggattca tcatccggat atttcctgcg atatctccat
    31141 aaaacaagaa ggcatcttgc aagaccaatc ccattttctt acgtaactct tccattggat
    31201 attctcgaat gtcatgatca tcaatcaaga tctggccttc ataaaattcg taaaaccgca
    31261 tcaatacgtt gatgatcgag ctttttccgc ttccggtgtg ccccactaat gcaacggtct
    31321 ctcccggatt agcgacaaaa gagatattgt tcaatacatt gtgctctcca tcataagaaa
    31381 agcttacatt gcgaaactcg atcttacctg aagtaatcgt ttgatctgct ccaggatttt
    31441 gttgcggtgc atatttttct gtatccatga ttttcaaaat acgactaccg gcaaccattc
    31501 catctgtaaa ggtgctcaaa aagtccatca tttgagacat tggattaaaa aatccctgga
    31561 cataggtcgt aaatgcatag atcagtccaa cttctacggg agaatgcagc gcatcgatcc
    31621 caaacaaagt caacgccaac gcaatcgcca atgcatatag aaaattgatg atcggactta
    31681 acagaatcga attcgttttg atcatcgcat agcgcgtgtc taaatactcg ttgttcactt
    31741 gttcaaattc tttagctaaa cgagcttctt ggcgaaactg ctggataata cgcatgccgg
    31801 aaatcgattc attcaatttc gtgttcagct gacttaattt ttcccgcata ttccggtaaa
    31861 tacgagaact gaatttctgg taataccaga tgacgattcc taaaatcggc aagaacacca
    31921 aagtccacaa ggcaatttct ccattgattt gaaacatggc aatgaaggaa gaaacgactg
    31981 caaagatccc agtcaatacc atcaaaaaga cataccaaaa ttcaaatagc gtttctgtgt
    32041 catttgtcac tcgagaaacg gtcgatcctg ccggtgtctg atcaaaataa cgcataccta
    32101 gtgtatgcag cttttcaaat aatcttaccc gaacatactg ataggtcttc aatgaagcca
    32161 tggaatacaa ataccattgg aaaaaccaaa tgatactttt gactatgact ccaaacaagt
    32221 acataccagc aaaaaacaaa ataacttgtg tcgtagccga ctgtttagcc agatgatcat
    32281 ccataaaagt ttggatgatc cttggcaata ggacattgat cacagacaga gcaaacgcaa
    32341 ataagatagc aacgatgaac gttcttctaa aaggtttggc gaacttcatc agtcgtttga
    32401 tgatcttctt ctgttcttta agtggaatcg attttgtcca ttccgattga taattttcat
    32461 ccatcaggcc tcgcctcctt ctatcttcgc ttctaattgc tgtttctccc acattcgttc
    32521 ataccagccg cctaatgcaa gcagctcatg atgggttcct cgttcaatga ttttcccttg
    32581 atcgacaaca atgatttctt ttgcatgcat cacgctgctt aaacggtgag ctgcaatgat
    32641 ggttgttttc tcttttctta tttcttttaa attatgcaag atagcttctt ctgttttggc
    32701 atctactgca gaaagtgcat catctaagat cagcaattca ggatttttga tcaatgctcg
    32761 agcaatcgaa atcctttgtt tctgtccgcc agataaagag actccacgtt ctccaactaa
    32821 ggtatcataa ccttttggca attccttgat ttcattgttg ataaatgcta aagaagccgc
    32881 atgctctact tcttcttctg aaagttttgg attagaaaaa cggatattat ctcgaatggt
    32941 catcgaaaac aagaagtgat cttgaggcac atagccgatt gatcctaata aagcattcaa
    33001 tgaatattct ttgatcgaac gtccgccaaa ttggattttt ccttgataat catcgtattc
    33061 acgcatcagt aatttgatga tcgtcgtttt tcccgcacca gttttcccaa caatacctaa
    33121 tgtttctcct tcttggatca caaatttgat ttgttctaat gctggtgatt cttctttcgg
    33181 atagttaaaa gaggaaatag ctacattcag ctcgcctttt gccggggtct gcactgcacc
    33241 cagttgttcc acgatatgcg tcttttcatg caaaagttca ttgacgcgat cataagaagc
    33301 atttcctctt tccaatacgt tgaacaatcg accaatcgca aacatcggcc aaacaagcat
    33361 accgatatag ctaataaatg agaccatctg cccgatcgag atcgtgcctt ccaagacata
    33421 ctttccacct aaaataattg ttgcaacata agacagaccg ataatcaagg taatgaatgg
    33481 atcaaataag gagtctaaaa aattgactcg tttgttttta ataattgcat cttcaatctt
    33541 ttcttcaaaa tccgcgagat cttctttttc ttgtccaaat gttttaatga ctttgattcc
    33601 agtaatactt tcttgtgtct tatcattgat actagaaaaa gcttcttgcg aatcacgaaa
    33661 agcatcgtgg agttttgacc ccagcactcg tgaagtaacg gctagtaaag gcagaggaat
    33721 cagcgcaatc agtgttaagc gccaatcaat aaacaaaatc atcgcaatga tcgttgttcc
    33781 gcccgtgatc actgaatctg caaaggtcaa gataccagct cctgctacat tttggattgc
    33841 attcaaatca ttcgttgcat gagccatcaa atctccagtc cgatattttt ggtaaaagag
    33901 attgtccatt tctgtaaaat gtttgaatag ctgctgccga agttctttct ccaaccgagc
    33961 tgcacttccc caaatattcg tccgccagat ataacgaaaa agatattgta agagagctgc
    34021 aaggatcaat aataagatcc accaggaaat gatctttatt gataaatttt tatcggcaat
    34081 ctcatcaatg atgatcccaa tcacctttgg aggaactaac tgtaccaagg ccactgcgaa
    34141 tagggagaaa atcccaatta aataatgttt cttttcctgt cgaaaaaacc aacctaattt
    34201 tctaaaaatc gacattttac tcctcctttg tctttatcaa gagcatagga ttgtctgtgc
    34261 ttgtcttttt tgctgaatag tctgaatagt ctaacaaatt acgaaataaa aaacggactg
    34321 aaaaaatcag cccgcaaaaa gtctttgggt cagaacctat tttttccctt ggttcttcat
    34381 ttgctgcatc atctggcgaa ttttcttctc agatggtttt tgccccattg acatcatcat
    34441 tgatcgcaac atgtcctcat taacgggagg atttttttct aaatagtctt tcatatattt
    34501 acgcgctagg aaaaagccac ctactgcgcc gattagcaaa gcaataatag caataagtac
    34561 gacaatacct gttgatacca tataaatctc tcctttctat ttccgaattg ctctcaatga
    34621 tttattttac taaagtttat tgaaaaaaga aaggcgtatt ctttagtttc atgcaaaaaa
    34681 tagtggtttt tttagagaag aacaaaattt ctttcattaa ccagcaagac acgccacgaa
    34741 aaaaaattag gcataccgaa tatttttctt taattttttc tttctgaggt gtagaatgta
    34801 atcgtagata aataggaaag gtggtttttt cttgaataat caatccaaca aacaatttca
    34861 taagcatcga cttattgaac atacaaatgg tttatcttta gctgaagtaa atggaacagt
    34921 ggatgtaccc aagggaaaaa gtttttttaa aacacttctt gcttactctg gaccaggtgc
    34981 attagtagca gtgggttata tggatccagg taactggtcc acttcgatca ctggtggaca
    35041 aaatttccaa tatttattga tgtcagttat tttgatgtct agtctgattg ctatgctttt
    35101 acaatatatg gcagcaaaat taggaatcgt gacccaaatg gatttggccc aagcaatccg
    35161 agcacggaca agtaagacat tgggtgtcat tttatggatc ttgactgaat tagccattat
    35221 ggcaacagac atcgcagaag tcattggtgc agcaatcgcc ctctacctgc tttttgatat
    35281 tccattagtg attgccgtat ttatcactgt gttagatgtt ttcgtcctct tattgctgac
    35341 aaaagtcggg tttcgtaaaa tcgaagcttt ggttgtctgc ttgattttcg tcattttatt
    35401 cgttttcgtc tatcaagttg gcttgtctaa ccctgattgg cacagtttgt ttgccggctt
    35461 tatccctaac agtcatacat tctcagactc accggtcatc ggcggtgaaa ctccgctgac
    35521 tggagcttta gggattattg gtgcaacagt catgccgcat aacttatatc ttcattcagc
    35581 agtttctcaa actcggaaga tcgatcacca agatgaagaa gatgtagcac aagcggtacg
    35641 cttttctact tgggattcta atatccagtt gtcaatggct tttctagtca atgcactgct
    35701 attgatcatg ggtgtcgctg tattcaaaac cggttctgtc caagatcctt ccttttttgg
    35761 tctgtatgaa gctttatctg atccatcagc tatgagcaat ggtatcttga ttgctgtcgc
    35821 aaaatctggt atcttgtcca ctttatttgc tgtagcactt cttgcttctg gccagaactc
    35881 tactattact ggtacgttga ccggtcaagt catcatggaa ggcttcatcc atatgcgtat
    35941 cccgatttgg ctaagaagac tagtcacacg attgctttct gtgattcctg tattgatttg
    36001 tgttttgttt acaagtacaa aaggaacgat cgaagaacat accgctttga ataacttgat
    36061 gaatgaatcc caagtttttc tggcttttgc attgccattc tcgatgattc ctttactgat
    36121 gatgaccaat agccaagcgg agatggggag ccgattcaaa aattcatttg ttgtaaaact
    36181 acttggctgg ttctctgtga ttagtctgac ttacttgaac ttacgaggat taccaggtca
    36241 aatcgaaagt ttcttcggcg agcaagccac ctcagctcaa ctagcattgg ctgatcaaat
    36301 cgcttatgta atcattgttc ttgtccttct cctgttggca tggacgattg gtgatttata
    36361 taaaggaaac aaaaaatatg aacagcgttt gcaagaaacg atagaagaaa tcgaatagaa
    36421 actgattctt ttacacaaag gaggttgtga cagaagtggg cagcttcaag aaataagaag
    36481 gaatttaaga aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt
    36541 tacttctgga acaccgtgta tcagaggttg tgaaaaagct gtcctgcatc aagtaataag
    36601 aacagccaaa caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat
    36661 gcaagctttt gaacaccgtt tattcggaaa aaggaactgc gacaagactt ttgtcacagt
    36721 tcccttttta tttagaagat aaagcttttt tcagattcaa tttactgaaa actagcatgc
    36781 ttgttactac gatgaattgt gttaatggca cgcctgcgcc agtgtagaca cttaaaaaat
    36841 aatccttttt ttctggcgca taagaacaaa accacgagac accgaaaaga aaaaccaatt
    36901 gtgtcagtat taatccggtg actacaatca aagaggaact ataataggca cgatcagctt
    36961 ttgcaaatac ggttcctagt atcataagta atatccctgc tattgaagca atcagcacgg
    37021 gaatggccag aacttggtaa atttttatca tgacattcgc gacttttgct gtttttgtca
    37081 actggaaatc aggtgttctc ggatcactgc tatcgatcca attatccatt agtggaagat
    37141 tcaataattc ttgggcctgt tcgacttgtt cttgcgtccc gaaactataa ttccctcctt
    37201 gatggaaatc tttgtatcgc catgcatagg catatcctga tttcagaaaa gcaaatacga
    37261 ttggcagatc attcatcgtc tttccatccc ctgatcccat cagatagaat tcttttttct
    37321 ttttgatagt tcccttttga taggcttctt ctaattcttg attgactttt ttccaaaaat
    37381 cattggtttc ttttgcattg ttttggtaat aaccagattc gcttaccaca tctcgcaatg
    37441 cccaaaaaat gatatctcct ggtaattctt ttacttttcc gccttgggtc cacgcatgag
    37501 tcgtatacaa ttcgtcaata cgagtagaaa cagtatgaaa tgtaggagat gcgtctaatg
    37561 ctttatttaa tgcagcatgc gagacccata tctcactgtt ttctttattt aagttcgttt
    37621 cgtcatctat tcggatcagc tgtttcatca aacgtccaaa ttcaccactc gaacggtcat
    37681 tgatggtggc aataccatat gttgtctcgt ttttatattc gatccactga tagacgatgg
    37741 agaaaccgac tagagggagt aaaaacaatg aaagtttcag ccattcttgt ttcgaaagga
    37801 gaaatggtcg ttcttttaac ttctttgctt ctttgaaaga aaagtcttgt cctagcaaga
    37861 tctgaacgaa actgatgcct aacgcgacta cagtaaaagg aagaagccag atggaatctt
    37921 cgcgaatgaa ccaaaagaaa ggaaaagaaa cagctaaccc taaaaaccac ggcagcaatt
    37981 ttctgcggga actatattta cggtaataaa tagctagtaa acaagcgata atgatctcaa
    38041 cagccgggat cacgagagca ttacgatacg ttcgagtgga atactcatgt gtaaaactaa
    38101 tcggagaaaa caaaaaataa aaatagatac ttaaaagaag atacttgttc ttcacgatcg
    38161 gtttaaacgc tagtgcaacc aatatgctgg caagaatact gaacatgccg aacaaaactg
    38221 cataagaaag atgcagttta tgtgcaagta ctaaaaacaa gctgtaggag atcccttttg
    38281 aaagtgtcgt agtcgtgtac tcccctagcc atttaccatc caaaatattt tgagcatagt
    38341 gaatcagcag ctgatcatca taggttgccg acatatctgt tccatagatt gctttactat
    38401 ataaatacaa ccgaataatt gtaaataata cggctatgcc aagaataaag tatgtatgtt
    38461 tcttttttag ttggttgatc aagggtttta aagtgttcag catcttttcg tcctttcaat
    38521 cacgttttgc tacttgcttg tgaatctttt cgttttttac ttttctatca gtataccctg
    38581 tttgtctgaa tttaaaaaga gggggcgaaa ggagcatggc gaaaacgact gcaaaaagca
    38641 ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc ccaatccttt tttgttgctg
    38701 tcagatagaa aacttaaagt gtgacttctt ctgctaataa ctcaggaaac acttcgggat
    38761 cgaatgcttg cgctttgaac atggcaattt cttttccata tggtgcgtaa gctttctttt
    38821 tatctttacc gacatatgga gtttctaaga ttttcgaaat gttgtcaaac attggatcat
    38881 ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa accgatattg gcatgccgat
    38941 ctttatgaga accttgtgga tttttagaat cgttgatatg gagtacttta agccgatcta
    39001 atccgatgac cttatcaaac tcttccatca cacctgagaa atcttctttt atattatatc
    39061 cggcatcatt cgtatgacat gtatcaaaag taacggataa tttttcattc aattttactc
    39121 catcgatgat ttgcgctagt tcctcaaatg ttcgaccgat ttccgtacct ttccctgcca
    39181 tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa aacttcattc aaaccctcaa
    39241 taattctagc aatcccacct tctgcccctg ctcctacatg agcgcctggg tgaagggtga
    39301 tttgagtagc acctaaagct tccgcacgaa caatctcttc tctcagaaat tgtacagcaa
    39361 atggaaacat ttctggtttg atcgtatttc ccaaattaat gatatagggc gcatgaacga
    39421 cgatatttgt tagtccgttt tctgccataa atgcttttcc ggcatcaata ttcatctctt
    39481 cgatagcttt tcttctagta ttttgaggag cgcctgtata gatcataaag gtcgaagatc
    39541 cgtaactggc tgcttcttct gccgcgccta acagcatttt ctttccgctc atgctgacgt
    39601 gtgagcctaa taacatggat ggattcctcc ttgaaaggca attgtttcga acaattccta
    39661 tatattggta agatgatgta caaagaaaat ggtttgtttt tctttgccct gttctccata
    39721 gtaactgaaa ctttctctaa agaaaagctt aagaactctt tcataaaaaa gatcatttcc
    39781 tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa acagccttgt ctgttttttt
    39841 gtcttattat ttgctataat tagcgaaata aaaccattag ttgaggtgac ccttttgcca
    39901 cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag aaaaatggtt tgttcctaag
    39961 gttattttta gagtgtttca atcattgact gtttttatta ctgttcttct tttgttgttc
    40021 gcagctctag gtatcggaat aggtgctggc tactttgcct atcttgtcga agacacacaa
    40081 ttgccgacta aaaaagaact gcagacagaa ctaggaaaca ttacggaaac gtcaaagatt
    40141 gtttatgcag ataatacaga gatatcaaaa atccagacag atctgatgcg gacgactatt
    40201 tcatctgata agatttcgcc tttattgaaa accgcgatta tcagtacaga agatgaatac
    40261 ttcgataaac accaaggtta tgtccctaaa gctgttttac gagcattagt ttcagaagct
    40321 actggcatcg gctcgtctgg aggatccact ttgacacagc aattagtcaa acagcagatc
    40381 ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga tcgttctttc tgcacaagta
    40441 gaaaaatact tttcaaaaga tgagatcatt gctacgtatt tgaatgtctc gccatttggt
    40501 cggaataata aggggcaaaa tattgctgga gtccaggaag ctgcaagagg aatctttggt
    40561 gtagatgcaa aagatgtgac tctgccacaa gctgcttata tcgctggttt gcctcaaagc
    40621 cctatcacgt atagtcccta cacgaataca ggcgcgctaa aagaagatct ttctgccggt
    40681 cttgccagaa aagattttgt tttattcagt atgtaccgtg aaggacaaat tacgaaagaa
    40741 caatacgaag aagcgaaagc ctatgatctt acaaaagact ttctcccaca acaaatcgcg
    40801 gaacagactg acagagaatt cttgtattat accgtgatga acgaagcaac aaggattatt
    40861 gcacaacaat tggcagaaaa agataatgct gatatgtcag attcatcggt cagtgaagct
    40921 tattatcaaa aagcacagca aacgatccaa aacaaaggat atacgatcca ttcgacgatc
    40981 gataaagata tctatgctgc attgcaaacc ggcgtagaaa attacggcta tatgttagat
    41041 gatggtgctg gtagccaagt ggagactgga aatgtattga tggacaatag gactggacgt
    41101 atctatggat tcgtgggtgg ccggaattac agccagaatc aaaataacca cgcctttgat
    41161 acagaacgtc aagctggttc atccatcaaa ccggttcttg tttacggtcc tgcaatcgat
    41221 atgggacttg tcggatcaga atcccgtgta tcagattatg caacgacttg gcaagaagga
    41281 gaaaatgctg gcgagaaaat cgtcaatgcg accaatgaag ggtcgaacac cttccaaacc
    41341 gtccgtgagt cactggaatg gtccaacaat atcccagctt atcacttata ccaagacgta
    41401 ttgaattcgg gtggatcgaa acaatatgct tacgagcact acttggcgaa gatgaactac
    41461 ccagcaaatg ataattgggg cgttgaatct gctcctttag ggacagtaga tgtaacgacg
    41521 cttcaacaga caaatggatt ccaggcctta gctaatggtg gcgtctacga agaaggctat
    41581 atcattgatt ccatcacaga taatgcaggt aatgtgattt ataaacatga aagtaattct
    41641 gtccgtattt attccgaagc aacggcctcg atcatgaatg acatgatgcg cagtgtgatc
    41701 aatgaaaaaa tcactacacc attcaaagat gtcatttcaa gcttaaacgg aaatctagga
    41761 aaagcagatt gggtcggaaa aactggttct accaacgaat acagagattc atggctgatc
    41821 gtctctactc cgtctatcac tatcagtagc tgggcaggac acgatgacaa tactggaatg
    41881 gacagccaag caaggatacg ttcggctaat tatcttgcca atctaatcaa tcaagcttac
    41941 caagcaaacc ctgagatttt cggcactgat gagaaatttg acttatcatc tgatgtgatg
    42001 aagaaaaaag tatctgggtt cacaggacaa acagctggaa aagttactgt caataaaaaa
    42061 tctatccaaa cgccaggaaa aacagtggaa tcactttggg caaaaaatgg tccgaagaaa
    42121 agcgccttta aattcggcgt tggcggtact gatgaaaact atacagatta ctggaacacc
    42181 gcttcagctt atgctagagc gaatccagag accaaagaat cagacgataa agatgattga
    42241 tctaatagat taatcagaat taaagggagc tgcgacaaaa atcttgtcac agctcccttt
    42301 ttccgaataa atggtgttca aaagcttgca tctacgatat taagacaaat ttatgtaaaa
    42361 atgtgaaaag ccattttcac aaaatttttc tatataactt tactaacgaa actgttttaa
    42421 tttcttttct tgtctttgga ataattcggt tagtttttct gcaaattgcc cttttactga
    42481 ttctttcaag tgtacatgtc ggttagaaaa ttcttgagca gcttcttctg cagtcatttg
    42541 gtggttttga tctgtcaatc gtttttctgc ccaaatttgc tgagatatcc ccataccaac
    42601 tgaacgtaca tttgatcgat gtagattttt ctctaattct tttagtaatt gcattaaaaa
    42661 ttcattcata agatgatttc tccatagtta tttgtctgat tttttgacga taggaacgtt
    42721 ctagttcgtc ttgctgctga tagagttttc gtcgttctac ttgtagggct tgttgataat
    42781 gctcctgata attcgaataa cttcgtcttg cttgaaacag aggctcatta tctaaagcta
    42841 agttctttaa tacatgatgc agtcgctgct cctcttgctc catcaatcgt aatccttgat
    42901 tcttaaagtg gatcaattca tcttcttgag cttctaattg tcttcgttca cttcgataat
    42961 ctctttccaa ttcttctagt ttcatctact tctcctttaa ttgaattgct gtgctaattc
    43021 tcgatctcgt gtcacgattt cattgatttt gtcttggatc tccttcacca atgtatcaaa
    43081 ggcctccccc atacgtcttg ccttattgat tcgcagcaga tagacttctt tcgcagaatc
    43141 gactaagttc tcttttgtgg cgacaacact agctaatgct tctatgattt cctgttcagt
    43201 caacgaaggg gcgacaacac gtgcttgccg caagccttct tgccatgcca ttttttctct
    43261 agctcaaaaa ttcctctttg attgatgcgg ataacttctt ccatagcgct tttcattttt
    43321 gctgataact tttccactac taacctagct tgtgtgtcat ctaagtaaat ttgttcatta
    43381 actgtcagcc ggccgccact taatcgtagt tttttacgta tgttgttcag ttcttgcaac
    43441 tgataggaaa acaactgggt cgcttggttt tctttctgtt gttttcgagc tttaacatta
    43501 ttttcatttt caggaataat cagctttcca ttttcatcaa acttccaaga ggttaaactg
    43561 tgggaagcaa gcagattcaa gaatgctgtt tccatactga tccgaacttc agcacctgtg
    43621 ccattaccac caaaattacc gataaaatcg gcgtgatttc ggtaattggt gagcataccg
    43681 ggattttctt ccacccattt ttttgcttct ggtgataaga gatcatacgg gtccggcccg
    43741 tttaagccga catttttcca ttgtttctct gctgctacat ataatgctaa gaatccacct
    43801 aatgaatggc cggtagtaga aatagcagct tctccatatt ccttttttac ttcattagca
    43861 aatgcaatcg ctgaattgat ttgccccgct gttttcaatc cactaacgga taaaatcttg
    43921 tttgttgttg ctttttcaga aaaacttgct cctggtctat tcccatagag attcatgata
    43981 ctccttgcat ccgtttccaa atctttccaa tcacttaagt tcgttcctgc aaaagcaatc
    44041 actacttgag aaagatccac tttaccgttc ttgattggcg ctacacccat cgcctgcatg
    44101 ccgtccaatg agtctttgat ttttaggatt ttaaaattat aactatcaat ttttttaata
    44161 gaattttctt tataagtatg atcatagtct ctgtgtttag gatctagcca gtaaacagcc
    44221 tcactaataa cactgtattg tttgtctgcc atcattaaat atattcctcc ttattattag
    44281 aaaaaattac tctgatttta tttactgtaa tcccctcttt ttgtatactt ttattttcta
    44341 cgccataatc tcctaattca tttcgttctt tccaaaatcc aaacgtaaaa taaacagact
    44401 catcgtgatt atttatcatt gtcacaaaca tacgatatgc tcccgtcatt tcatttcgag
    44461 cagatttttc aatcttcact tccttgatat ccgcaaaagt atttttcaaa actttgactg
    44521 actgtttttc tactgcgagt aaatctttct gtctttgtgc ttctcttttg tccatataga
    44581 ctttccctcc gattcctaat atgacgatta ataaaatggc taagctgagt tttcttattt
    44641 tccgagacaa gaacgtgcct cctttctttt tatgtaagta ttgatacctt cattatttaa
    44701 gtacgcgaaa gtttgtagga atttttttat ttttgaaatt tttctccaat ttgctatgga
    44761 cgattggcgc tgcagccatc gcctgcatgc catccaatga gtctttgatt tttaggattt
    44821 ggaattttgt ttctccaaag tttttaatgg cgccttcttt gtaactttta ctatattgag
    44881 gatgttttgg atctagccaa taaacatgtt cacttatata tctatactct tcgtctttta
    44941 ctgtcacact tcctccgcct ctccattaga ataaataact cttattttag gagtagtaac
    45001 cccttcgttt tgtatttctt cattttctac accataactg ctaatttcat tcatttgttt
    45061 tgaaaaaaaa taatcaaaat aaacagattt cccttcatta tttgtcattg tcacaagtgc
    45121 accataacat ccagtaacag ggtttttttt aaattcttct accttcactt ccctgatatc
    45181 tgcaaaggta tttttcagaa ctttgacgga ttgtttttct actgcgagta aatctttctg
    45241 tctttgtgct tctcttttgt ccatgtaaaa ttttcctccg attcctatag caagaatggt
    45301 caaaatgatg actatggtct ttcgtctttt cttaggcaaa tagaagtctc ctttcgggtt
    45361 atgtatgatg gattgtttct cctttaagat aagtacgcaa tcgagcctgg atttttttgg
    45421 attttttaga gttacttttc tacttttcta ctttcttttt tatcacgttt tcattttaaa
    45481 gtaattttga tagtagtgaa caaaatgtag catctacgca cagattgtgt cgtatacgat
    45541 ggacgtaagg tttgtttccg ccattttctc tttttttaaa gtcacaaaaa aaaggggtta
    45601 tgacaaaatt ttgtcataac cccttttctc gaataaacgt tgttccagaa gttggccact
    45661 tttggaacaa cctctaaatg attgatcttt tattatttag ttgatccttt ttcttcgatt
    45721 ccataaggca agataatcgt tttttcttcg acttcttctt tattcatcat tttcgtcaat
    45781 aggcgcatgg ataccgctcc taaatcgtat aatggttgtg tgatacttgt caaacgagga
    45841 cgagctactt ctgtcagtaa agaattgttg cttgtgatga tttcgaattc ttctggtact
    45901 tttacgccac ggtctaataa accatccaag ataccaatgg ctaattcatc gtctgaaaca
    45961 tagacagctg ttgcaccgct attatggatc cggtcaacta gagacagacc atctttaaag
    46021 ttgtattgtg cctcaaagat taatccttcg ctatatggta gattgttttc gcttaatgct
    46081 tctttatagc cttttagacg gttttgacca ttgataggat caatcagcgc accgctgaca
    46141 aaagcaattt ttttattgcc gctttttgct aatttattta ctgcatcttt tgttgctgat
    46201 acataatcaa tgtttacgct acctacttgt tcatctggat caatggaacc agctagtaca
    46261 acaggtgttt ttgaacgtga aaattctcca cggatttcgt ctgtgatatg gtgacccata
    46321 aagatcacgc cgtccacttg cttagccaat aaattattca atacattgac ttctttttga
    46381 tcgttgccat ctgagtttgc taagatgata ttgtatttgt acattgttgc tacatcgtcg
    46441 ataccacgtg ctaaagaggc aaagaacata ttgctgacat caggaatgat taccccaaca
    46501 gttgttgttt tcttacttgc caagccacgt gcgactgcat ttggacgata atccaagcga
    46561 tcgataactt ccaagacttt ttttcttgtt gctggtttga cgtttggatt gccatttacg
    46621 acacgtgaaa cagtagccat cgaaacattt gcctctctcg ctacatcata gatagtgatt
    46681 gtttgctttt ccatttgtgt tctcctattc atattttata tgttttctga aaatacgtct
    46741 tacatttcta aaataggata tcaaataatc tcaacaattg caaccgtttt atctctgttt
    46801 tcatgaaaat ttttactttt ttcagtttta agaacgttgg tcttttaatc aatcagtggt
    46861 acaattattg aatacaaaat taatttagaa agtaggttgc caaatgaata acgtaaaaat
    46921 caatgaacta cgtcaatgga tggcacaaca ccaagtagaa ttggcttata tcagcgaccc
    46981 tggccacatc gcttactttt ccggatatga aagtgatcct cacgagcgtg tactagctct
    47041 ttttatccct cttgaacaag atcctttcct tttcactcct gctctggaag ttgaagatgc
    47101 tcaaaacagc agctgggcgt acgatgtacg tggctactta gacagcgaaa acccttggga
    47161 gatcatcgga aaagagcttc gcgcacgcta tgggcaacct ggtaatgtag ggattgaaaa
    47221 gaatgctctg actgtcgatc gcttcgatgc tatctctgat atcttgacag gtgcagattc
    47281 ttacactgat ctaacttcta ttatccagcg tctgcaactg atcaagacgg tagaagaaaa
    47341 agacaaactg atcgaagctg gtaaatgggc cgatgttgcc tttgaaattg gcttcaaaac
    47401 agtaaaagaa ggcgtgactg aacaagcaat tatcgcagaa atcgaatacc aattaaaaaa
    47461 acaaggtgtt tcacaaatgt ctttcgacac cttagtcctt gcaggagcaa atgctgcaag
    47521 ccctcatggt acaccaggaa gcacgaaggt ttcaccaaat gaactagtgt tattcgatct
    47581 tggcgttgtt tggaatggct attgcagtga tgctactcgt acagttgctt atcaaaaacc
    47641 aacagaattc caagaaaaaa tctacaacat cactcttgaa gcacagcttg ccgcacaaga
    47701 ggcagttcgc ccaggtgtga cagcaggtga acttgatcaa atcgcacgaa acgtaatcaa
    47761 cagctatggc tacggcgaat atttcaatca ccgtttaggt cacggaattg ggacaaccgt
    47821 ccacgaattc ccttctcttg tagaaggaaa cgacttagtg atcgaagaag ggatgtgctt
    47881 ctctcttgaa ccaggaatct atattcctga aaaagtaggg gtacgtatcg aagattgtgt
    47941 gtatgtgaca agtgatggtt gtgtgccatt cacgactaca ccaaaagaat tacttgtttt
    48001 agagtaatga aaataaaaaa ggtgggagct ggtccgctct caccttttta ttctagaaat
    48061 gagtattctt agtgattggt agtcgaatct gtcattgtat ctttcatttc ttttttagct
    48121 tcttttgcat cttcttttgc cattttcaac atttcttttg tgtcatctgc aactggtttt
    48181 gcttctgaca ttccttcatt gaccgtctct cctgctttat ctgcagcatc ttttacatcg
    48241 atcatgatat cttctgaagt atctttagca atactaccta attcgtctgc tggttttttc
    48301 agatcttctg ctgttttttt gaaacgatta gataaatcag ctgattgttc tttgaggttt
    48361 aacaagatat catctgattc acttcctgct tcctctgctg attcacttac cttttgctta
    48421 acattacctg ctagatcccc tgcttgttga gaaagatctt gcgctttttc ttttgcaatg
    48481 cctgttaact cagctgattt ttcttttgcc atatctgtgt aatctgaagc catgtccatc
    48541 aaatcatctg cttgagcagc caattcatcc cgaagttctt taccagattt gggtgctaac
    48601 aataatgcgg cgattgctgc ggctgtacct ccaactactg cacctaataa aaatccacct
    48661 tttttagcca ttcttatttt cctcctgtcg tttcagtatg ttttttgttt ttaaaaaatt
    48721 tcatcgctgt tccaccgact tttccggcaa cagtcgcttt agctgtcgtt tttccgattc
    48781 cgccgacttt cgtcacgatg tttttactag aatgattaag ttccgataca ctttcactta
    48841 aatcagcaac tgctgtgaac aatggatcga tcgttgcgac ttttccattg atatccaaga
    48901 gcagctcatt acttttgacc agcaagcctt ctacttgtct agataaaaga tctacgtctt
    48961 ttgtgacgat ctcaattgtc gtatttgctt ctgcgacagt ctggtctact ttttctaaag
    49021 ttttggaaac ttgcaacaat acgcgaatga tgaaaacgac aagtatcgca aaggcgattg
    49081 ctgcgatcaa tcctgcaatt tctcctcctg acatgatcaa actcccttct tgtctctctt
    49141 ttctataagg atagcatttt cgtgtttttt aaacaaataa aaacgagctt caactacttt
    49201 ttattcaatt agcagctgaa gctcgttaga aaaaaatgat tattttttct tttcttcttt
    49261 gatttctgtt attccttttg gattatgatc gatcttgctt ccttctgctt ctaatgtttt
    49321 acctaaagac atgatgtatt cacgcaaagg tgcccctact tctggatgtg tcagaccata
    49381 ttcaatactt gttttcataa aaccaaattt gtcaccaacg tcataacgtt tacctgtgaa
    49441 tttacgggca aatacgcgtt gtgttttatt caacgtatcg attgcgtcag ttagttggat
    49501 ctcatttcca gcacctggtt gttgttctga taatacatca aagatttctg gagtcaataa
    49561 gtaacgccca ataatagcta aatcacttgg tgctttgctt ggatctggtt tttcaacaaa
    49621 gtttttgaca ttgtataacc catcttccag tacttcccct gggttgataa ttccgtattt
    49681 tgaagtttca tcgtgaggaa cttgcataac ggcaatcgta gaagcgtgtg tacgttcata
    49741 gtcatcgatc aattgctgtg tcaatggtac ttcgtcttcc atgatatcat cgcctagcat
    49801 aacgacaaac ggttcatttc caataaaggc tcttgcttga agaacggcgt gtcctaaccc
    49861 ctttggatga gattgacgga tgaaatgcaa gttgacatct gtcgtttctt ctactaattt
    49921 caataattcg atttttcctt tttcacttag gtttgtttct aattcgatat ttgaatcaaa
    49981 gtgatcttcg attggtcgtt tagcttttcc tgtaacgatc aagatgtctt cgattcctga
    50041 tttcaacgct tcttcaacga tgaattggat cgttggtttg tccacgattg gcaacatttc
    50101 ctttgccatc gcctttgttg ctggtaaaaa tcttgttcct aatcctgctg ctggaataac
    50161 tgcttttttt actttcatat acattcgctc cttcaaaaaa ttgttaatga gaattcattt
    50221 tcttttttcc catcacgtaa catgatttct tttgcggctg ttttgatttc tttttgttca
    50281 tacagcactt catagattgt ttgagtgatg ggcatttcga tttccatcat ttgggctagt
    50341 tccatcgctg cttgagtagt attcacacct tcaacgacca ttcccatatt ggctagcacc
    50401 tcttctagag gctgtccttg tcctaacaag ttgcctgcgc gccagtttct ggaatgaaca
    50461 cttgtgcaag taacgactag atcgcctact ccgcttaagc caataaaggt cagcgggttg
    50521 gcacccattg ctacacctaa acggctgatc tcagctaatc cgcgcgtcat gattgccgct
    50581 tttgcattat cgccaaaccc taagccgtga atagcaccag ctccaatggc aataatattt
    50641 ttcaaagctg cccccatctc tacaccaacc acatcagggt tagtatagat tcgaaaataa
    50701 tggttcatga acagctcttg tacatatttt gctgcttttg catctgtgct tgccgctgtg
    50761 atcgtcgtaa tgtcatgctt tgctacttct tccgcatggc ttggcccaga aagaacagca
    50821 acagcttttc ttttttctgt cgggatttcc tctaacaaga tttcagagat tcttttatgc
    50881 gttccctgtt ctaatccctt acttgcgtgg atgatcactg gcttcgtatt caatttggca
    50941 actagttctt ttgcaacaga acgtatcgct ttcgtcggga taacgaatag taccgcatct
    51001 gcatctttta ctgcatcttc catatcggtg aatgctcgga tggaatcggg tagatgatga
    51061 tctggtaaat aacgttgatt ggtgtgttgg gtgttgattt cttctacttg tgaagcacga
    51121 tgtccccaaa tccgaacgtc atgtccgttt tcagcgagga cttgtgctaa agcagtcccc
    51181 caagagcctg gtcctaaaac cgctactcta taattcattt taccactcct gaaatttttt
    51241 tcatttttaa cgctctaaaa atcaaataat ttgttgattt tttcctaatg agcggtcata
    51301 atccggtaaa tttccttttt ttctacgcca aataaacaga atcaatgtgc cgacaagtaa
    51361 aacagcagac aataattgag aaatgcggat cacacctaaa aagtaaaggc tgtctgtgcg
    51421 taatccttcg ataaagaaac gtccgaaact ataccacatt agataacata aaaggacttc
    51481 tccttttttc aaaagatgcg gtttcttccg caaaaccaaa agcagaataa atccaatcaa
    51541 actccaaagt gattcataaa gaaaggttgg ttgtcgatag atcccttcga tatacatgtt
    51601 atcaatgatg aattgcggta aatgcagact ttctaagaaa cttcgtgtcg tgtctggacc
    51661 atacgcttcg tggttcatga aatttcccca tcgtccgatt gcctgcgcca gtaatacact
    51721 tggtgcagcg atatctaaaa aggtccacaa agaaataaaa tgatatttgg taaaaaagta
    51781 cagtgtgatt gcacctgcaa tcaatccccc gtaaatcgct aatcctcctg aacggatatt
    51841 gaatatttgg attggatcag ctatatattg gggaagatcg aataaaacat aatacaaacg
    51901 ggcaccaata atggaaatcg gcaatcccca caacataaaa tctgtcacgt catctgcttt
    51961 taatcctacc cgaacagctt ctctgctgct gagccacatg gcaataatga ttcctgatac
    52021 gataatcaat gcataccagt agataggaat tcctacaagc tcaaaagcaa ctcgattgat
    52081 ttgtgctaac atattgtcac tccttgctag tcattgcctg agttttcttc aatcaagcga
    52141 cttaatcttt cttcaaatgt tttcgttgcg tcaaagccca ttgtacgcgc gcggaaattc
    52201 atcgcggcta cttcaatgat gattgcaacg tttcgtcctg ttttgacagg gatttttatt
    52261 tgtggaatgc ccacttccgc gatttcaacg gtcgtgtctt cagaacccaa acggtcgtat
    52321 ttcttatctt tcgcccatgc ttctagataa acagctaact ggacttgcat agagcctcgt
    52381 accgcactcg caccgaaaag attcattaca tctatgatcc cgatccctcg aatctcaatc
    52441 agatgttcca ggattttagg aggttctccg attaccgtca gttcatcttg ctgatagaca
    52501 tctactcgat cgtctgcgat caatcgatgt ccgcgcttaa tcagttccaa cgccgtttca
    52561 cttttcccta tcccactgtc cccttgaatt agtacaccta atccgtagac atcgactaac
    52621 acaccgtgga cacttgttcg aggggctaag cgcccatcta aataactaga taattcacct
    52681 agcagccgag acgttgagat aggcgaacgc agcacagcta tcccattttc attggctgct
    52741 tggatcattt cttccggagc cggcaatcct cttgaaataa taaatgcggg catatcttca
    52801 ctacataatc ttctcatgat gatcaaacgc tcttctggga tcatccgttc cgcaaaactg
    52861 atttctttac tgccaaatag ctgtaaacga ttatgagaat aataattaaa ataaccggtc
    52921 aactctagtc ctgggcgtga gatgtcccca gtcacgatga cgcggtctaa acttttctcg
    52981 tcgcctgtga cgatttccaa agataatttt tttactaatt ggctaacttt tacaacttct
    53041 gacattcatg ctacctcatt tctattagag tgttctctac cattttatca ttgtctagcc
    53101 aaaagtacta ccaattaaaa aaaacgtttt tatttaatta ccaatgtaat agaaaacgca
    53161 ttcttaatta tatatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct
    53221 caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat aattacgttg
    53281 attgctgaca ctggaaataa atgtctgatt cacttctcta aaatccattg atttcggcag
    53341 tccattacga cgcagaatcc cgttagaatt
//
LOCUS       DAFEZB010000009        53072 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFEZB010000009
VERSION     DAFEZB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.009"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..243)
                     /locus_tag="LP859_000599"
     CDS             complement(<1..243)
                     /locus_tag="LP859_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL3028576.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(358..936)
                     /locus_tag="LP859_000600"
     CDS             complement(358..936)
                     /locus_tag="LP859_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3028577.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1013..1543)
                     /locus_tag="LP859_000601"
     CDS             complement(1013..1543)
                     /locus_tag="LP859_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL3028578.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="LP859_000602"
     CDS             complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="LP859_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL3028579.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2400..2795)
                     /locus_tag="LP859_000603"
     CDS             complement(2400..2795)
                     /locus_tag="LP859_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL3028580.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2792..4204)
                     /locus_tag="LP859_000604"
     CDS             complement(2792..4204)
                     /locus_tag="LP859_000604"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL3028581.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="LP859_000605"
     CDS             complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="LP859_000605"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL3028582.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4823..5062)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5059..6519)
                     /locus_tag="LP859_000606"
     CDS             complement(5059..6519)
                     /locus_tag="LP859_000606"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL3028583.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6689..7846)
                     /locus_tag="LP859_000607"
     CDS             complement(6689..7846)
                     /locus_tag="LP859_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL3028584.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7880..9250)
                     /gene="radA"
                     /locus_tag="LP859_000608"
     CDS             complement(7880..9250)
                     /gene="radA"
                     /locus_tag="LP859_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL3028585.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9313..9840)
                     /locus_tag="LP859_000609"
     CDS             complement(9313..9840)
                     /locus_tag="LP859_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL3028586.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10070..10876
                     /gene="proB"
                     /locus_tag="LP859_000610"
     CDS             10070..10876
                     /gene="proB"
                     /locus_tag="LP859_000610"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL3028587.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10873..12120
                     /locus_tag="LP859_000611"
     CDS             10873..12120
                     /locus_tag="LP859_000611"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL3028588.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12196..12540
                     /locus_tag="LP859_000612"
     CDS             12196..12540
                     /locus_tag="LP859_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028589.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12657..12983)
                     /locus_tag="LP859_000613"
     CDS             complement(12657..12983)
                     /locus_tag="LP859_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028590.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13356..14557)
                     /locus_tag="LP859_000614"
                     /pseudo
     CDS             complement(13356..14557)
                     /locus_tag="LP859_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14674..15189
                     /locus_tag="LP859_000615"
     CDS             14674..15189
                     /locus_tag="LP859_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3028591.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15388..15834)
                     /locus_tag="LP859_000616"
     CDS             complement(15388..15834)
                     /locus_tag="LP859_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL3028592.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15881..17359)
                     /gene="msr(C)"
                     /locus_tag="LP859_000617"
     CDS             complement(15881..17359)
                     /gene="msr(C)"
                     /locus_tag="LP859_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL3028593.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17713..18342)
                     /locus_tag="LP859_000618"
     CDS             complement(17713..18342)
                     /locus_tag="LP859_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL3028594.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(18326..18790)
                     /locus_tag="LP859_000619"
     CDS             complement(18326..18790)
                     /locus_tag="LP859_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3028595.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19277..20545)
                     /locus_tag="LP859_000620"
     CDS             complement(19277..20545)
                     /locus_tag="LP859_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL3028596.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20776..21513)
                     /locus_tag="LP859_000621"
     CDS             complement(20776..21513)
                     /locus_tag="LP859_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3028597.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21580..22149)
                     /locus_tag="LP859_000622"
     CDS             complement(21580..22149)
                     /locus_tag="LP859_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL3028598.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22277..23149
                     /locus_tag="LP859_000623"
     CDS             22277..23149
                     /locus_tag="LP859_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3028599.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23491..24363
                     /locus_tag="LP859_000624"
     CDS             23491..24363
                     /locus_tag="LP859_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3028600.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24360..26318
                     /locus_tag="LP859_000625"
     CDS             24360..26318
                     /locus_tag="LP859_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3028601.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26569..27420)
                     /locus_tag="LP859_000626"
     CDS             complement(26569..27420)
                     /locus_tag="LP859_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3028602.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27543..28010
                     /locus_tag="LP859_000627"
     CDS             27543..28010
                     /locus_tag="LP859_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL3028603.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28273..28755)
                     /locus_tag="LP859_000628"
     CDS             complement(28273..28755)
                     /locus_tag="LP859_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL3028604.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28855..29718)
                     /locus_tag="LP859_000629"
     CDS             complement(28855..29718)
                     /locus_tag="LP859_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL3028605.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30316..31164
                     /locus_tag="LP859_000630"
     CDS             30316..31164
                     /locus_tag="LP859_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028606.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(31335..31676)
                     /locus_tag="LP859_000631"
     CDS             complement(31335..31676)
                     /locus_tag="LP859_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL3028607.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            31762..32469
                     /locus_tag="LP859_000632"
     CDS             31762..32469
                     /locus_tag="LP859_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL3028608.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            32456..32779
                     /locus_tag="LP859_000633"
     CDS             32456..32779
                     /locus_tag="LP859_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL3028609.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(32868..33905)
                     /locus_tag="LP859_000634"
     CDS             complement(32868..33905)
                     /locus_tag="LP859_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3028610.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(34352..35335)
                     /locus_tag="LP859_000635"
     CDS             complement(34352..35335)
                     /locus_tag="LP859_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL3028611.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(35751..36521)
                     /locus_tag="LP859_000636"
     CDS             complement(35751..36521)
                     /locus_tag="LP859_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028612.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(36514..37449)
                     /locus_tag="LP859_000637"
     CDS             complement(36514..37449)
                     /locus_tag="LP859_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028613.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(38043..39692)
                     /locus_tag="LP859_000638"
     CDS             complement(38043..39692)
                     /locus_tag="LP859_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL3028614.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(39887..40783)
                     /locus_tag="LP859_000639"
     CDS             complement(39887..40783)
                     /locus_tag="LP859_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3028615.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(40776..41456)
                     /locus_tag="LP859_000640"
     CDS             complement(40776..41456)
                     /locus_tag="LP859_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3028616.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(41457..42239)
                     /locus_tag="LP859_000641"
     CDS             complement(41457..42239)
                     /locus_tag="LP859_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028617.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(42232..43149)
                     /locus_tag="LP859_000642"
     CDS             complement(42232..43149)
                     /locus_tag="LP859_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028618.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(43152..43436)
                     /locus_tag="LP859_000643"
     CDS             complement(43152..43436)
                     /locus_tag="LP859_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028619.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(44450..44857)
                     /gene="arsC"
                     /locus_tag="LP859_000644"
     CDS             complement(44450..44857)
                     /gene="arsC"
                     /locus_tag="LP859_000644"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBL3028620.1"
                     /translation="MRKIYFLCTGNSCRSQIAEGYGKAILPTDEFEIKSAGIEQHGLN
                     PRAVQVMAEEGIDISTQTSDLIDMDYFNQADLIITLCGDAKDKCPVIPKGIKHVHWDL
                     EDPAKATGTEEEIINKFREIRDDIKSRVANLLD"
     gene            45215..45574
                     /locus_tag="LP859_000645"
     CDS             45215..45574
                     /locus_tag="LP859_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL3028621.1"
                     /translation="MKNEICEISCVHEDKVLLGKEKLQTEDISSLKSVFKILADENRL
                     KIIYALSYEDELCVCDIAATIEATVATTSHHLLSLKKNGIVVSRKVGKLVYYSIKNDK
                     FVQLLNSKLYLEDEVLV"
     gene            45571..47688
                     /gene="cadA"
                     /locus_tag="LP859_000646"
     CDS             45571..47688
                     /gene="cadA"
                     /locus_tag="LP859_000646"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL3028622.1"
                     /translation="MSEKKQVYRVEGLSCTNCAAKFEKNVSQIPKVTDAKVNFGAGKI
                     SIEGEATIAEIEAAGAFENLKVQSEHDTEPRIETKEPFVKRNWNLLVSLFLIILALGS
                     QVVNGEDALLTEGLFILAIIIGGFSLFKEGFSDLLKLNFSMESLMTIAIIGAAIIGEW
                     TEGSIVVILFAISEALERFSMDKARQSIRSLMDIAPKEALIRRNNVEQMINVSKIEVG
                     DIMIIKPGQKIAMDGQVIKGHSSVNQAAITGESVPIEKNINDDIFAGTINEEGALEVK
                     VTKHVNDTTIAKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMVIAALIVIVPPLFFN
                     GDWNTWLYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLKA
                     IAFDKTGTLTKGTPTVTDFTTVDSKDEEKYFSIITALESYSQHPLASAILKEADNRAI
                     SYKSVVVDEFTSITGKGIQGNIEGITYLVGSPKLFESILTDNSKIIENYQRLQQQGKT
                     AMLLGTDKQILAVIAVADELRESSKAVIEKLHDLGIEHTIMLTGDNATTAQSIGKQTG
                     VTEIKGDLMPQDKLDYIKSLKETYGKVAMVGDGINDAPALAASTVGIAMGGAGTDTAL
                     ETADVALMGDDLQKLPFIVKLSRQTLRIIKQNITFSLGIKLLALLLVVPGWLTLWIAI
                     LADMGATILVTLNGLRLMKVKSK"
     gene            complement(47909..48721)
                     /locus_tag="LP859_000647"
     CDS             complement(47909..48721)
                     /locus_tag="LP859_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028623.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGTQENQYILEMLQKKAGKLDPKIEGLIVDELKQ
                     AGLVSEKEMDRMIEFFIINLIPPTEQDQFTYQCIDEKKQLYLGSTKFGKEAQLYLHHD
                     NQNHILHILPFTFSNFSVDRLLVYLANQESYSYFGKHLPKELVEKAILLYSINQLTVD
                     VRIAPLVYSKETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNVAYPSSK"
     gene            48883..50139
                     /locus_tag="LP859_000648"
     CDS             48883..50139
                     /locus_tag="LP859_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3028624.1"
                     /translation="MNQVNQHDLGESIRVSREERGWTQRYLAEKVGISRSLLSKVEKG
                     TRQLSAEKLNLILDSLQEEIVPVNRVLIDYLTIHFFSNQHLKLIEAIIGMPIERFEEL
                     DYAPKGYIGQYVWNQVITIRYSIDDTVKGTVMEFSGQGCKHLAMRLKTAKSNWQEFFR
                     KVLDYQGNFTRIDFTLDDFVGSLSIPELKRKVTLGHVWTTFQVSESHGGTDIINNESN
                     GETLYLGSKKSQCRFCFYQKDYEQRKRRGISLEEAEVKNRFELRYRKEKAQSLAKIIS
                     KTHDLTKLFFELLNGAICFYDRAPNDPGAKVDAKWAAFIGNHGATTISLETIPQSFEK
                     SMNWLIHSVSPTLAFIQEVDNHFDSNLINEIISCGELSSRQQKILENLIAEPDYYQEE
                     VEFYIQCLQNMKAAKIHKKSKAQLTH"
     gene            50229..50426
                     /locus_tag="LP859_000649"
     CDS             50229..50426
                     /locus_tag="LP859_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL3028625.1"
                     /translation="MKNTITTYELPYLLTREQASHFLGIDPKSFDKFVRANDELERFM
                     IGRQERYTVTSLINFIKTHSI"
     gene            50532..51581
                     /locus_tag="LP859_000650"
     CDS             50532..51581
                     /locus_tag="LP859_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3028626.1"
                     /translation="MPSIIKRGNSYRAQISLYNHGQHKKLTKSFSSKKEATRWALENE
                     LEKGNGKQLAERTTTFADFFENWIHIIKKNDVKETTFQNYQRTSQVVKKLFGDIQLKD
                     LNDIVVQRKIDKYAETHSRKTTHEVLLKIKTALRDAYARGYLATDFANLVKTRGKVLD
                     KRNRVLSITELKKLRTYVINHRENEFNILVLLALETGMRRGELLGIRPEDLFEYGIHV
                     RRSLSPTSEDTSLKTKNSRRDISINQEVYDILKSVPIKDNGYFFDPDGFQQSAKLARL
                     LKKLDIPKTTFHGLRDTHASFLFSQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLNSLSNL"
     gene            complement(51879..52499)
                     /locus_tag="LP859_000651"
     CDS             complement(51879..52499)
                     /locus_tag="LP859_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028627.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(52580..52990)
                     /locus_tag="LP859_000652"
     CDS             complement(52580..52990)
                     /locus_tag="LP859_000652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028628.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT        15728 a        10914 c         9491 g        16939 t
ORIGIN      
        1 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
       61 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
      121 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
      181 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
      241 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttacca
      301 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
      361 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
      421 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
      481 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
      541 tgcttctttt aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa
      601 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
      661 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt
      721 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
      781 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
      841 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
      901 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
      961 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
     1021 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
     1081 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
     1141 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
     1201 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
     1261 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
     1321 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
     1381 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
     1441 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
     1501 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
     1561 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
     1621 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
     1681 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
     1741 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
     1801 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
     1861 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
     1921 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
     1981 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
     2041 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
     2101 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
     2161 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
     2221 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
     2281 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
     2341 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
     2401 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
     2461 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
     2521 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
     2581 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
     2641 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
     2701 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
     2761 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
     2821 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
     2881 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
     2941 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
     3001 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
     3061 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
     3121 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
     3181 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
     3241 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
     3301 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
     3361 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
     3421 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
     3481 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
     3541 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
     3601 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
     3661 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
     3721 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
     3781 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
     3841 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg
     3901 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
     3961 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
     4021 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
     4081 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
     4141 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
     4201 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
     4261 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
     4321 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
     4381 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
     4441 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
     4501 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
     4561 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
     4621 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
     4681 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
     4741 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
     4801 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
     4861 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
     4921 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
     4981 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
     5041 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
     5101 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
     5161 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
     5221 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
     5281 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
     5341 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
     5401 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
     5461 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
     5521 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
     5581 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
     5641 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
     5701 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
     5761 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
     5821 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
     5881 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
     5941 cataccatcg cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
     6001 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
     6061 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
     6121 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
     6181 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
     6241 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
     6301 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
     6361 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
     6421 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
     6481 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
     6541 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
     6601 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
     6661 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
     6721 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
     6781 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
     6841 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
     6901 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
     6961 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
     7021 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
     7081 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
     7141 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
     7201 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
     7261 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
     7321 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
     7381 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
     7441 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
     7501 tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
     7561 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
     7621 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
     7681 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
     7741 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
     7801 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
     7861 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
     7921 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
     7981 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
     8041 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
     8101 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
     8161 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
     8221 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
     8281 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
     8341 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
     8401 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
     8461 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
     8521 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
     8581 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
     8641 cgattttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
     8701 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
     8761 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
     8821 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
     8881 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
     8941 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
     9001 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
     9061 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
     9121 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
     9181 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
     9241 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
     9301 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
     9361 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
     9421 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
     9481 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
     9541 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
     9601 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
     9661 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
     9721 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
     9781 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
     9841 cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt
     9901 tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt
     9961 tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac
    10021 aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga
    10081 aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa
    10141 tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa
    10201 tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt
    10261 acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca
    10321 agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca
    10381 gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac
    10441 attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc
    10501 agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat
    10561 ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc
    10621 agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga
    10681 aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag
    10741 taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg
    10801 aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa
    10861 ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa
    10921 caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc
    10981 atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc
    11041 gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat
    11101 gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat
    11161 gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg
    11221 accggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc
    11281 aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta
    11341 ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag
    11401 tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta
    11461 gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg
    11521 gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag
    11581 ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac
    11641 gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa
    11701 aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta
    11761 gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt
    11821 gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact
    11881 aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa
    11941 gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt
    12001 ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg
    12061 ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa
    12121 tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac
    12181 tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg
    12241 aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga
    12301 agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg
    12361 ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat
    12421 tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt
    12481 tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa
    12541 aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt
    12601 agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac
    12661 cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg
    12721 ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct
    12781 tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc
    12841 tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca
    12901 cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact
    12961 tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa atgaatttta
    13021 gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa
    13081 aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat
    13141 aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag
    13201 agttaaacga cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt
    13261 aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact
    13321 tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa
    13381 taattacggt gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa
    13441 aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct
    13501 ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt
    13561 atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac
    13621 acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa
    13681 ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga
    13741 gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa
    13801 ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag
    13861 aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac
    13921 gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt
    13981 accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc
    14041 catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt
    14101 gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt
    14161 gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc
    14221 agcattccga tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat
    14281 cctgtcatca atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca
    14341 ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt
    14401 ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga
    14461 tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt
    14521 gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat
    14581 acatgattta ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat
    14641 aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta
    14701 tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt
    14761 tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca gcagggttat
    14821 gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa
    14881 ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat
    14941 gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg
    15001 ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat
    15061 gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt
    15121 atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt
    15181 tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc
    15241 aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat
    15301 cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa
    15361 ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact
    15421 tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt
    15481 ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc
    15541 gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat
    15601 ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc
    15661 gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa
    15721 acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat
    15781 caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta
    15841 ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta
    15901 ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt
    15961 aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag
    16021 cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg
    16081 gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata
    16141 acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg
    16201 attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt
    16261 gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc
    16321 cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg
    16381 cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc
    16441 tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg
    16501 gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga
    16561 gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt
    16621 tagaggaaga caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt
    16681 ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt
    16741 tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat
    16801 aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg
    16861 atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat
    16921 aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt
    16981 ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt
    17041 cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta
    17101 attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg
    17161 tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca
    17221 aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac
    17281 gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat
    17341 ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga
    17401 tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc
    17461 tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt
    17521 cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat
    17581 ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga
    17641 cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta
    17701 actgaaaacc ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta
    17761 accttcttct accgcttttc ctattgataa aataataact ggaacataac ggtctttatc
    17821 caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata
    17881 gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac
    17941 tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg
    18001 ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc
    18061 gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt
    18121 ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc
    18181 gaccacaaag cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat
    18241 catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct
    18301 tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg
    18361 tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt
    18421 tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt
    18481 cacaatgctc gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt
    18541 tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag
    18601 taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt
    18661 cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa
    18721 agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt
    18781 ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa
    18841 tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat
    18901 agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc
    18961 acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag
    19021 tagaaaattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat
    19081 attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca
    19141 caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa
    19201 acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct
    19261 ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg
    19321 gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat
    19381 ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat
    19441 aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt
    19501 ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag
    19561 caatcccttc atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg
    19621 tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg
    19681 gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat
    19741 aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg
    19801 tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca
    19861 ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat
    19921 tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat
    19981 tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc
    20041 cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac
    20101 ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag
    20161 atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat
    20221 gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg
    20281 gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag
    20341 ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct
    20401 ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg
    20461 tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc
    20521 taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa
    20581 ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt
    20641 tctttttgat aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc
    20701 attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat
    20761 attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga
    20821 catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa
    20881 tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg
    20941 atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg
    21001 ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac
    21061 ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga
    21121 tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt
    21181 gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat
    21241 gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat
    21301 aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc
    21361 tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt
    21421 agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat
    21481 gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc
    21541 tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg
    21601 tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac
    21661 agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc
    21721 tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg
    21781 ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac
    21841 tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac
    21901 atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac
    21961 cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc
    22021 atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat
    22081 cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag
    22141 gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta
    22201 atacttataa aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa
    22261 aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga
    22321 aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca
    22381 actgaaagat ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat
    22441 acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt
    22501 agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg
    22561 cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc
    22621 gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact
    22681 ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca
    22741 gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt
    22801 agcaaagcaa accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg
    22861 gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc
    22921 gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt
    22981 tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc
    23041 actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc
    23101 tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt
    23161 ttccttcgct ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat
    23221 aacataagtt tcttaattta tttcttcctt ttatttctga taattccagt aaattactat
    23281 aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa
    23341 taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt
    23401 ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt
    23461 gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat
    23521 gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt
    23581 gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa
    23641 ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt
    23701 gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat
    23761 gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc
    23821 gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt
    23881 gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa
    23941 gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc
    24001 agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa
    24061 acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga
    24121 ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa
    24181 atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg
    24241 accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc
    24301 gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat
    24361 tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg
    24421 ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg
    24481 ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg
    24541 ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg
    24601 tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact ttattgaata
    24661 tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta
    24721 ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg
    24781 ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc
    24841 tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg
    24901 ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt
    24961 tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag
    25021 gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt
    25081 atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt
    25141 acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt
    25201 ttgcttcatt ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt
    25261 ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct
    25321 tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga
    25381 aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga
    25441 tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg
    25501 aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg
    25561 cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata
    25621 actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa
    25681 tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg
    25741 taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag
    25801 cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg
    25861 aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg
    25921 atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa
    25981 tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa
    26041 tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa
    26101 aaggcgaacc attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga
    26161 ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg
    26221 tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag
    26281 caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt
    26341 tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat
    26401 aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa
    26461 gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt
    26521 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    26581 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    26641 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    26701 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    26761 tgcttcaata atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc
    26821 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    26881 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    26941 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    27001 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    27061 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    27121 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    27181 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    27241 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    27301 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    27361 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    27421 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    27481 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    27541 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    27601 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    27661 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    27721 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    27781 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    27841 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    27901 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    27961 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    28021 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    28081 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    28141 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    28201 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    28261 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    28321 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    28381 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    28441 ttctgctaaa gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt
    28501 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    28561 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    28621 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    28681 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    28741 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    28801 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    28861 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    28921 tgtaaaagag atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc
    28981 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    29041 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    29101 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa
    29161 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    29221 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    29281 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    29341 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    29401 ttgtatgccg tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    29461 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    29521 atctagaaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    29581 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    29641 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    29701 gttcagatct ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa
    29761 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    29821 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    29881 tatgttttac ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg
    29941 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    30001 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    30061 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    30121 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    30181 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    30241 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    30301 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    30361 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    30421 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    30481 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    30541 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    30601 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    30661 atagaacatc cgcactttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    30721 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    30781 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    30841 tcgcttattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    30901 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    30961 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    31021 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactct
    31081 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    31141 gataaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg
    31201 ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca
    31261 tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag
    31321 gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctggata gattttccgg
    31381 cattgttgat agattttatg atacttttct acattttgta agagaggttc cgctgcatct
    31441 gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccaccc taaccctagt
    31501 gcagcaatca ttgttgcgcc taatgcaggt ccttcctcca tacttaaaca atagaccgtt
    31561 gcatcgaaaa tatcagcttg gatttgtagc catgcaagat ttttcgcgcc tcctcccacc
    31621 gaaacaatac gtgtaaatct gcgacccgct agttctttac gtttcaatcc caccattgac
    31681 aaatgctcat taaaaattta gaatattcac aagtttaacg acaattatga aaatttatgt
    31741 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
    31801 acgttaccta ccgttttcgg ttatatcggt attggacttg catttggtat cgttgggaaa
    31861 gctgccggat ttcatccact agtcgtcacg ttgatgtccc tgctggtcta tgctggttct
    31921 gcccaattta tcacagtcag catgcttgct agtcacagcc cattgctttc catcgttttc
    31981 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
    32041 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
    32101 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
    32161 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
    32221 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
    32281 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
    32341 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagcaac
    32401 ttagtgattg tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
    32461 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
    32521 ggtcctacca tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt
    32581 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
    32641 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
    32701 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc tttccctttc
    32761 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
    32821 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
    32881 ggaccactaa caacgacttt tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt
    32941 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
    33001 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
    33061 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
    33121 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
    33181 acaaggtgct tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
    33241 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
    33301 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
    33361 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc ggatccgttg
    33421 atcactaaga tttttttccc gcgattggca ttttcttctg gagttagacc aaatttttca
    33481 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa
    33541 agattttttg gtgcaagagc gacaatacgt tcgtcaacta gttggtattc ttgattgctt
    33601 cctgctcttg ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
    33661 tcgtcaccta cagcaacaac tttcccgact ccatcaaaac ctaagatacg taaagcattt
    33721 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
    33781 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
    33841 tttggatcat cgatcggcaa cccttcataa aaaccgacta ccttcattaa ttccttttct
    33901 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtg ttcatttcaa
    33961 tatatatagt atgctatttg ttccaccttt tttattcatc ataattattc tcttacttaa
    34021 agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac
    34081 ctcttataaa cggtgttctc ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac
    34141 atgagaagct gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa
    34201 cctctgatat aaacggtgtt caatcaactg accctcggta ttagctcaaa aaacgacaaa
    34261 atatgaagag ctatttttgt ttggctgttc ttattacttg atacaggaca gctttttcac
    34321 gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt
    34381 ctttttcaaa ggtctctaag atctctcgta cttcatcagt agatttcgta ttcatcaact
    34441 gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta
    34501 gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac
    34561 gaagtagtcc gatcagttct ttttctgtat gttccctcgg gtctttttca aatgcataag
    34621 gattcttgaa aatccctcgg ccaatcatga tgccatcaac gccatatttc tcggccaatt
    34681 cttctcctgt ctggcgatcg gggatatctc cattgatcgt gattagtgtt tgtggagcga
    34741 tttggtcacg taaagcaatg attttaggaa tgacttccca atgtgcatct actttactca
    34801 tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac
    34861 tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag
    34921 gcaaaccgcc agctttagct gcttcaatca attgtgccgc tacttctgga cgaaggatca
    34981 agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga
    35041 ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt
    35101 ctccccaaat atgggcaacc atcggctgtt catcttctgt aaatgttaat cttcctcgta
    35161 cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga
    35221 catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca
    35281 ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt
    35341 tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta
    35401 tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa
    35461 aaaaaagact gtgacaaaaa tcttatcaca gtccattttc cggaataaat ggtgttcaaa
    35521 agcttgcatt tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt
    35581 ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt
    35641 caatcagctg accctcggta ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc
    35701 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc
    35761 aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaataac
    35821 acaagtacga tcatcgatgt tgctactggc acatcgatcg ttccaaggaa tccgtaacgg
    35881 aaaccggaga tcatataaac gatcggattg acttttgaaa cagcttgcca aaagggcggt
    35941 aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta
    36001 ggcacgatcg atacatcatc ataagactga gcaaaaatcc cgttgagcag tcctgctaat
    36061 gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga
    36121 agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc
    36181 acaccaccaa tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc taccaagatt
    36241 tcttcgatat ttttctgaaa tttttgagag aagaaggacg aagaaacatt ggcatacgaa
    36301 ctagtgatag cagacatcat aatcaaacca ggaacgatga attccatata tgaaaaacca
    36361 cccatgtcac cgatccgccc accgatcatt tttccgaaaa taacaaaata caaggatgtc
    36421 gtgatcactg gcggaaccag tgtttggacc catatacgca aatagcggtt cgtttccttg
    36481 atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt
    36541 attttcttct gtgattttca agaacagttc ttctaaacga ttcgacttat tcctcataga
    36601 aagaactttg attccttgtc ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg
    36661 ttcaacttcg acagcgattg tctgctcatc ttcaaatgta caagcatatc cttcaatcac
    36721 aggtttggta tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gttttgacag
    36781 aagctctttc atacttgtat tctcgatcag ttcacccgat tggatgattc cgatgttgcg
    36841 acaaagcatt tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg taccattttc
    36901 gttcaattct cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt
    36961 tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct
    37021 tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga
    37081 ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc
    37141 tgcttgattc acgacgattt gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg
    37201 aaccaatcca atctgttgct ttgctcgaac tagatccttg tccagatcat aaccgaagac
    37261 attcactttt ccagatgttt tattgactaa agaagtaata atcccgatcg ttgtagactt
    37321 cccagctcca tttggcccta atagcgcata gaaatctcct tctttcaccg aaagatcgat
    37381 tccccgcaat gcttcgaccc cacttccata gatctttttc aaattattta tttcaagcgc
    37441 atttgccatt tatttcctat ctccttatag acatgattct taattgtatc atggaataca
    37501 gcagatgaaa aaacaacttg gcttccacac aaaaacacac ctgctaacaa agaggagatg
    37561 tgttttctag ttcatgatca tcttcgatag aataactatc taaacaactc tggaactttt
    37621 aaaatcataa aagtaccgac agcaactact acgatgacat atagaagatc cttataactg
    37681 aggttcttgt cttttttatt catgttaagc tttcctccgt ccttttatcg aaagcttttt
    37741 tcttttattt taaccgaaat aaataaaatg gaaatagtta ttttcaattt ctgttttcgc
    37801 tgtttgtttt acggagaact ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt
    37861 ttcgataaaa gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa
    37921 tcttccaaga tcatctggac agcagactcg ttcccattga attctgccac ttttttggca
    37981 tcaatgtact tcttttgata atcgtctttt tggacccacg catcatgctt cgtttgataa
    38041 agttaatcaa acggatacca ctgacgctgt tcatcgaaca acaaactttc tttttgggcc
    38101 tgttcaacta tggatttagg ataattcaaa actttcagca aggcaatcgc atttcgggtc
    38161 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
    38221 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
    38281 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
    38341 gcaatccgtt cgatcgtatt tgttcctttt aaaatctcat ctacgaagca gtagcagcgt
    38401 tctttggaag ccacttggtc cagcaaacgt ttgatcgatt tgatttctga aacaaaataa
    38461 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
    38521 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
    38581 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
    38641 tgccagttga ctggattaac taccgcttct tttaacaatg gatgatagat atctttcgct
    38701 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatcg gcatataggt gcgataattc
    38761 aaaacggctg ctgcaacttc caattctccc agcaattccc acagcttgat ggcttcagtt
    38821 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
    38881 atgttgacat attcaaaaat caattctgct tcactcccgc tcttcattcg aaatgaaaaa
    38941 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttt ttgaagtggt
    39001 gtcgtcattt ttgctaactg attggcggct gaaatcgttg aaaccaaata actcatgcta
    39061 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaag
    39121 ccaccgattg ctagatagat tcctagtact tgccctaaaa gaagcaaacc tacacccacc
    39181 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
    39241 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
    39301 tggattcttt ctctcagttg aggattttcc tgataaaaag caattagttt ttctaactga
    39361 taatcttctc caaaatcaaa gctacgtaaa cgctgataca acgcttctga acctacacta
    39421 gaataggtac tgttgatccc ttcaaatact tcgaacatat ccaagtcata ccaagtaaga
    39481 tcgtcgattt cactatccca tgaacggaat tttttctccg tgagccacgc atccttcaaa
    39541 ctttcttctt tatcaaatct cggttgataa ggaatttttc cccaattact acgaactgtt
    39601 tcctttactt tcaaacgtga aaaaatgtct aaaacaacta ataaaataac aactgcaata
    39661 ataattaaaa cgataaattc tctttggccc atatccctct ccgttccttg atttgttagt
    39721 tttagtatag cactgttgcg ctatttgaaa agggggtaca aaaataactg tcttcgttac
    39781 ctaattcgta tcaatcacac caaaataaaa acaccttcca aaaaagaaga tgtctttaaa
    39841 acgagataca attttttgtc attcacttca ggtaaattag agccaattaa accctccgca
    39901 acttgacgga gatggagaag atgcccccgt ctaatctagc ggtcatctct cctcccattc
    39961 cttccaccaa tttttttgac aggtaaagcc cgattccgct agattccgta tttgtgcggc
    40021 tggtattttc ggtataaaaa cgctggaaga ctttttcaac agaggtttgc gctcctggtt
    40081 tgataggatt ggccactgtt aatcgaatga atgaatcctc ttttgaaaat gtcacctttg
    40141 cttgtccatc tgcataacgc aataaattct gtatgatatt ttgcagcact cgcagcaaca
    40201 tgagcggatc tgctaataca gttgtattta tcgagtgttc agcgaacgct acctcgatat
    40261 ttttctcaga aaattgttca taaaaagaga aaaatacttc ttccacctct ttagaaaaag
    40321 aaagcgtatg gagatccatt tctttttgct tcgtctctat tcgagcaaga tcataaaact
    40381 gatccgtcat actgagtaat cttgcaacag attgtttgat gatcgttaaa tacttctccc
    40441 gtttctccgt atcctctacc tgttccagta attgtacata accgttaata gcagtcaaag
    40501 gagttctgag atcatgagaa aggccgacga tcgatagtct gatgttttct tccatttctt
    40561 gaaatgcttg tcctttttgt tcattttcat ccacccatgt attcagtgca tctactaggt
    40621 ccattagatt cttttctcga aattcgactg aaagacgacc accatattct tgtcgttcag
    40681 aaagagtggc gattttctta gttaatctct tgatttccct ttgcaggaag aaaataaaac
    40741 ttcccataaa gataacaacg agaagcaaca gccacttaat caaatctaaa ccccactccc
    40801 cacaccgtat gaatatacac agcatgtgcc tttgctagtt tccctcgaag attggaaata
    40861 tgcatattca ctgttttctc actatcaaaa tatggttctt gccaaactgt ttcataaaga
    40921 tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag atcgaattct
    40981 cttcctgtca aatggacagc cacaccattc acatttacat ccctcgactc tttgtccagt
    41041 aagatttcct tgtaatagat gcgttgcgac agatctttta cttggacaga agtctgatga
    41101 cgaagttgga tgccgattcg taacaacagt tcttcgatat caaatggttt cgtcacatag
    41161 tcatccgctc ctgctgccaa caaggaaact ttatctcttt gatcgctttt tgctgaaaga
    41221 acgatgatcg gaacattcga tgtctgtcgg atttctgcta acaatgcttc acctgaaagc
    41281 cctggaagca tgagatctag aaggatcaaa tcgacaggat atgtgcttaa caataatttt
    41341 ccttccgtac ctgaataagc atcaagaagt ttgtatctct gttcaagaac ctcctccagt
    41401 aaagaatgaa cagcttcatc gtcttcgata atcaaaatag tttccagcag gaacacctac
    41461 ttaatgtcag atttttggaa aataaacatt cctagagctg atgataccac gattgttact
    41521 agactgacca gaatcatttt cggtgcttgc gaataagttg cttgttgcgc aaagtccgta
    41581 tacatgagcc acgtttccgt aaaccactcc gttagaaatt ccaagccttt gatttttgcg
    41641 aaaacaccgg gaacgaaggc cgttcccatg atataaagaa aagaaagaag caaactgaca
    41701 ctggtacttc tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc taatatcagc
    41761 agaagcaata caggaaatac ttttagaaca taatttccat agtttttgat tccctcaact
    41821 tcaccaaatc cgaaagcaag tgtgtaagca atcatggcta gccccgtaat caaaaccacg
    41881 ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca cataataagt cattcgattc
    41941 cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa attcttcccc aatataaaag
    42001 cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca ttttttccac cattaaaatc
    42061 cctgttgcac cgttgatgac tgtatccgtt gcttggttta attcattgcc gtattttgta
    42121 tgcatatacc attcgttcca agaataaaaa agcggaagta cgaccatcag tcctagtgag
    42181 acccacattt tacggctctt ccatactttt attttttcag cagttaatag attaaacatg
    42241 tggattgcct ccagttaatt gtaagaaata atcttccagt ttttgcccaa ctaggtggat
    42301 accatccaca ggaacttttg cctctaccaa tgcttgattg atggctgcta cttggtcaat
    42361 tccttcaaaa agctggataa cacggctaga ttggacaaaa taatccctat aaccaagaga
    42421 gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca atttttataa attgacgaga
    42481 ttcttgtgcc aattcttctt tcgataattc tttgatcaag tgtccttcat gcaaaatacc
    42541 gtagtgagtg gcgatctgtg acaattcatc tagcaagtgg ctagaaagca agacggtgat
    42601 gccccgttct tttaccaaac gttggatgat ttctcgcatt tctgccatcc ctgctggatc
    42661 catcccattc gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc
    42721 aatagctaaa cgctggcgca tacctaaaga gaaattcttt gctttctttt tccctgtatt
    42781 ttccaatcgc atcattttca gcaggtcttc gatttctcta tccgacacac cgccgttcgc
    42841 tgcttggata cgtaggttat ctcttgcagt taattctgga taaagagcag gggtttcaat
    42901 cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc gttccaaata attcgatcga
    42961 accttcatca atcgagatca aacccataat agttctcata aaggtagact tacccgctcc
    43021 attctcccca atcaaaccat agatcattcc acgtttgatt tcgatcgata ctttatctaa
    43081 tgctttggca gcaccgtatt ttttggttat accagttgct ttcaagaccg tttcatttcc
    43141 aaaatccata cttatccttc tttcttattg ataagtgaag tatagttgac gggtataaag
    43201 aaagcagcca gaaatgtata aagaaagtgt aaattttcca attgaatttc aagaaaaatc
    43261 agactgtctc gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct
    43321 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacatcag acgtccttgt
    43381 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
    43441 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
    43501 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
    43561 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
    43621 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
    43681 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
    43741 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
    43801 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
    43861 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagat gtgactcgta
    43921 cttattacaa gtacttctat caatagaaat ccaattatat caacattaca aacatctaaa
    43981 aaacagtttg atatggataa aataggatta atattgatat tacttgaacc aatttgaacc
    44041 aaagatgaat ggtagttgaa tacaattttc ttactagttt atcaatcttt tttagagtta
    44101 ttcctcccct cttatttatt tgagaccgta ttgatggctg gatagctgtc taataggggt
    44161 ttcaaggcca gaattagcta agctactaga ttctcgaata attgtataag ccggtcacac
    44221 ctacttaaat gtgattgaga aggtcaaata tagggtcaaa aaatactcgt cagtatgtca
    44281 gatagataaa tccaatttta cgccaaaagc agatcctttt ctcttaaaat agtaagagcc
    44341 atcgaagatt tatgtttcac ttcgcacggc ttttacttta attttccaat ttatcaaaag
    44401 gacattttac gtctttttat cttttaccga gatagatgac agttgctact taatctagca
    44461 aattcgctac acgacttttt atatcatctc taatttctct gaatttatta atgatttctt
    44521 cttctgttcc agttgctttg gctggatctt ccaaatccca atgaacatgc ttaatccctt
    44581 ttggaattac tggacattta tctttggcat cgccacatag cgtaataatt aggtctgctt
    44641 gattaaaata atccatatcg atcaaatcag atgtttgcgt agagatatct atcccttcct
    44701 cagccattac ttgaacggct ctaggattta acccatgttg ttcaatgccg gcgcttttaa
    44761 tttcaaattc atctgttggc aaaatcgctt tcccatagcc ttctgcaatt tgactacgac
    44821 aagaattgcc tgtacataaa aagtaaattt ttctcattat tatttcctcc gatatcagac
    44881 aattctgatg gattttgaat gaaccaattt ttttgtgcga ttagatattt taactaacag
    44941 taacatgact ggcacttcaa ctaagatgcc aactgccggc gcttatgtaa aaatatagat
    45001 aatcatctat gttttgtcaa atgcttctgt ctttcagttc aaattaatta gtttattatt
    45061 tgcattcgat tcaatctaat ttgaaaatca ctacttatat agatgcatcc atacaatcta
    45121 tataactcct cacatattca aattaaaatt tgattattct attaatctat gatacactac
    45181 attcaaatat ccattagaat aaaggaggag ctgtatgaaa aacgaaattt gtgaaatctc
    45241 atgtgttcat gaagataaag ttttactagg aaaagaaaag ttacaaactg aggatatttc
    45301 gagcttaaaa agtgtcttca aaattttagc tgatgagaat cgcttaaaaa ttatctacgc
    45361 cctaagttat gaggatgaat tatgtgtatg tgatatcgca gcaactatcg aggcaaccgt
    45421 ggcaacaacc tcacaccatt tattatcatt gaaaaaaaat ggaattgttg tgagtcggaa
    45481 agtagggaaa ttggtttatt actctatcaa gaatgataaa tttgtccaac tactaaattc
    45541 caaactgtat ttagaagatg aggtgctagt atgagtgaaa aaaaacaagt ctatcgggtt
    45601 gaaggattga gctgtacgaa ttgtgcggca aaattcgaaa aaaatgtttc tcaaatacca
    45661 aaagttaccg atgcaaaagt aaatttcggt gccggaaaaa tctctatcga aggcgaagcg
    45721 actattgcgg aaattgaagc tgcaggagct tttgagaatc ttaaagttca gtccgaacat
    45781 gatacagaac ctcgcattga aacaaaagag ccttttgtga aaagaaactg gaacctattg
    45841 gtttcactgt ttttaattat tttggcatta ggatctcaag tagttaacgg tgaagatgcg
    45901 ctattaacag aaggattatt tatactagcg attattatcg gcggatttag tttgtttaaa
    45961 gaaggatttt cagacctatt aaaattgaat ttctcaatgg aatctcttat gacaattgca
    46021 atcattggtg ctgcaattat tggcgagtgg actgagggct caatcgttgt tatcttattt
    46081 gcaattagcg aagctttaga acggttttca atggataaag caagacaatc aatacgttcc
    46141 ctaatggata ttgctccgaa agaagcatta attagaagaa ataatgttga acaaatgatt
    46201 aatgtttcaa aaatcgaagt aggcgatatt atgatcatta agcctggaca aaaaattgct
    46261 atggatggtc aagtcattaa aggccattcc tcagtaaatc aggctgcgat taccggtgaa
    46321 tctgtcccta ttgaaaaaaa tattaatgat gatatctttg caggaacgat taatgaagaa
    46381 ggtgcacttg aagtcaaagt tacaaagcat gtgaacgaca cgacaattgc caaaattatt
    46441 cacctagttg aagaagcaca aggtgaacga gcacctgctc aagcatttgt cgataaattt
    46501 gcgaaatatt atacacccac cattatggtg attgccgcat taatcgtcat cgttccccca
    46561 ttatttttta acggagattg gaatacttgg ttgtatcaag ggctctcttt attagttgtt
    46621 ggctgtcctt gttctttagt aatctcaaca cctgtgtcta tcgtttcagc aattggaaac
    46681 tctgctaaaa atggtgtttt ggtaaaaggc ggtatttatc ttgaagaaat tggtggcctc
    46741 aaggcgattg cctttgacaa aacaggaaca ttgactaaag gaacaccaac ggtaacagat
    46801 ttcactacag ttgattcaaa agacgaagag aaatatttct caattattac agcattagaa
    46861 tcatattctc aacaccctct cgcgtcagca atcttaaaag aagctgataa tagagcgatt
    46921 tcttataaat cagtagttgt tgacgagttt acttcaatta ctggaaaagg aatacaagga
    46981 aacattgagg ggattaccta tcttgtaggg agtccaaagc tttttgaatc tatcttaaca
    47041 gataactcaa agattattga aaattaccaa agacttcagc aacaaggaaa aacagcaatg
    47101 ctcttaggaa cagataaaca aattttagca gtcattgcag tagctgatga gttaagagaa
    47161 tcaagtaaag cagtcattga aaaattacat gatttaggaa tcgagcatac gataatgctg
    47221 actggtgata atgctacaac tgctcagtca atcggaaaac aaacaggtgt aactgaaata
    47281 aaaggcgatt tgatgcctca agataaatta gattatatta aatcacttaa ggaaacctat
    47341 ggaaaagtcg ccatggttgg tgatggtatt aatgacgcac cagcattagc tgcctcaaca
    47401 gtcggtatcg caatgggtgg cgctggaaca gatacagctt tagaaacagc tgatgtagct
    47461 ttaatgggtg atgacttaca gaaacttccc tttatagtta aattaagtcg acaaacattg
    47521 agaatcatta agcaaaatat taccttctca ttgggaatca aactattagc attattactt
    47581 gtagttccag gctggttaac gttatggatt gctatcttag ctgatatggg agcaactata
    47641 ttagttacct tgaacggctt acgactgatg aaagttaaat ctaaatagac aagtagtagc
    47701 ataaaaagag gaaagactag cgacggcagc gccgtataag ctagtctttc ctctttttaa
    47761 tgctgttgca ggacggcgta gccggactgc atgggcaagc ccctctttct aacagggggc
    47821 taacaaagaa caggaaactg tgacaactac ccatttggga gtgtcctagc agtgttttct
    47881 ttgtcacatg cttatcaatc ttgtcacctc attttgatga tggatacgct acgtttaaca
    47941 gacgttcttt ctcgacttcc atcacgaatg gtagataaag atattgttgt tctagtagat
    48001 attgattgtc ctctgtgtac tgataagaag ctgtttcttt tgaatagacc aacggggcaa
    48061 ttcgcacatc gacggtcaat tgattgatcg agtatagaag aatggctttt tcaactagtt
    48121 ccttgggcaa atgtttccca aaataggaat aggattcttg atttgctaaa tagacgagta
    48181 agcgatccac actaaagttg gaaaaggtaa agggcagaat gtgcagaatg tgattttgat
    48241 tatcatggtg taaatagagt tgagcttctt taccaaattt agttgaacca aggtaaagct
    48301 gctttttctc atcgatacat tgataagtaa attggtcttg ttccgttggt ggaatgaggt
    48361 ttataataaa aaattcaatc atccgatcca tttctttttc actaacaaga ccggcttgtt
    48421 tcagttcgtc tactattagc ccttcaatct taggatcaag cttacctgcc ttcttttgaa
    48481 gcatttctaa aatgtattgg ttttcttgag taccatacaa aagatattcg ttggtgacat
    48541 ggcctatttc tgcaatttgg tgaagggttt cggtttttgg aaggttaatc ccacgttccc
    48601 agttattgac tgtacttcgt ggtaaatcac caattagttt gccaaatttc tccatactca
    48661 actttaactc ttgacgaatc tgccgaattc gattgcccac tgcttgcttg tctatctcca
    48721 tgaaaactcc tcctcatgat aagttcttaa attcagtata cgcttgtact taaaaaaagt
    48781 caaaatgtat tgacttaaaa taaatacagg cgtacaatcg atttgtactt aaaataagtc
    48841 tgatggttct ttgacaattt aatagcagaa ggaggttttt ctatgaacca agtcaatcaa
    48901 catgatctcg gggaatcgat tcgagtttct cgagaagaaa gggggtggac ccagcgatat
    48961 ttagcagaaa aagtggggat ttcgagatcg ttattatcga aagtagaaaa aggaacgaga
    49021 caattaagtg cagaaaaatt gaatcttata ctggatagtt tgcaagaaga gatcgttcca
    49081 gttaatcgtg ttttgattga ttatctaact attcactttt tctcgaatca acatttaaag
    49141 ttgattgaag caattattgg catgcctatt gaacgctttg aggaactgga ttatgcgcca
    49201 aagggctata ttgggcaata tgtttggaat caagtaatca cgattcggta ttccattgac
    49261 gatacggtaa aaggaaccgt aatggagttt tcaggtcaag gctgtaaaca tcttgccatg
    49321 cgtttgaaga ccgcaaaatc aaactggcaa gagttcttcc gtaaggtatt agattatcag
    49381 ggaaacttta cacggattga ttttacttta gatgattttg tggggagtct cagcattcca
    49441 gaactgaagc gtaaggtaac actaggccat gtgtggacca cttttcaagt gagtgaatct
    49501 catggcggta cggatatcat aaacaatgaa tcaaatggtg agaccttgta tttaggctca
    49561 aagaagagtc agtgtcgctt ttgcttttat cagaaggatt atgaacaacg aaagcgacga
    49621 ggaatttctt tagaagaagc agaagtaaaa aatcgctttg aactacggta tcgaaaagag
    49681 aaagcgcaaa gcttggcaaa gattatttca aaaacccatg atttaaccaa actattcttt
    49741 gagttactta atggagcaat ttgtttttat gaccgtgctc caaatgatcc aggtgcaaaa
    49801 gtcgatgcca aatgggcagc ctttattggc aatcatggcg caaccaccat ttctttagaa
    49861 acaattcctc aaagctttga gaaaagtatg aattggttaa ttcacagcgt ttctcccacc
    49921 ctcgcattta ttcaagaggt agataatcat tttgattcaa atttgattaa cgagattatt
    49981 agctgtgggg aacttagttc aaggcagcaa aaaatattag aaaatttgat tgctgagcct
    50041 gattattatc aggaagaagt agaattttat atccagtgtc ttcaaaatat gaaggcagca
    50101 aaaatacaca aaaaaagcaa agcacagctt acacattaaa atgtataaac gtactccgcc
    50161 ctagacaagt tgagtatagc acatttcctt gtgtcagcca aaaaacattt ttggaaagga
    50221 aataaactat gaaaaataca ataactactt atgaattgcc ctatctactt actagagaac
    50281 aagcttctca ttttttaggg atcgatccaa aatcttttga taaattcgtt cgcgcaaacg
    50341 atgagttaga gcgttttatg ataggaagac aagaacgata cactgtcact tccctcatta
    50401 attttattaa gacgcactct atctagctaa aatatcgtag gatggttgat tagatgcttt
    50461 tcgccatgta gaatgacggt gtattcaact agcatttatc aaccatcttt cagtgaaagg
    50521 atttgttata aatgcctagt ataattaaac gtggtaactc atatagagct cagatctcac
    50581 tttacaatca tggtcaacat aagaaattga caaaatcatt tagctctaaa aaggaagcaa
    50641 ctcgttgggc cttagaaaat gaattggaga aaggaaatgg aaaacaatta gcagagcgta
    50701 caacaacatt tgctgacttc tttgaaaact ggattcatat cataaagaaa aacgatgtaa
    50761 aagaaacgac ttttcaaaat tatcaaagga cttctcaagt ggtgaagaaa ttatttggcg
    50821 atatacagtt aaaggatttg aacgatattg tagtacaacg aaaaatagat aagtatgcag
    50881 aaactcattc tcgtaaaacc actcatgaag tactattgaa aatcaaaacg gcattacgtg
    50941 atgcttatgc tcgtggctat cttgctaccg attttgcaaa tttagtcaaa acacgaggga
    51001 aagttttaga taagagaaac cgtgtacttt ctattaccga attaaagaaa ctgcgtactt
    51061 atgtcattaa tcatcgagaa aatgagttta acattcttgt actccttgcc ttagaaactg
    51121 gcatgagacg cggagagctt ttaggtatac gtcccgaaga cctctttgaa tatggtatcc
    51181 atgtcagacg ttcccttagt ccaacttctg aagatacctc gttaaaaaca aaaaattcaa
    51241 gacgagatat ttccattaat caagaagtct acgatattct aaaatctgtc ccgattaaag
    51301 ataatggata tttttttgat cctgacggct ttcaacaatc tgctaagctc gccagattac
    51361 tgaaaaaact agatattcca aaaactacct tccacggcct aagagatact catgcttctt
    51421 ttctattttc acaagatatt gatattgcct atgtatcaaa acgcttaggt catatcaata
    51481 ttcaaacaac gcagaactat tatcttgaat tgatgccaga aaaaaagcac cagcaagatg
    51541 ctgatgcttt aaacctctta aattctttgt caaatttata atttgaacca acttgaacca
    51601 aaaaattctt gtaaacgttg atacaataag gaattgatta acgttttgag aactgtgaag
    51661 ctttacgggc tttcttaaga cctggttaaa caaaactcta ttttattgca ttttacagga
    51721 atgtatttca tgcgaaatta ataagtcttt gtttatagag ttttactagg gtataaaaag
    51781 ttgtatgtta tcattacctc aaaacattac ctcagcttgg cgagttggtt gattgaagaa
    51841 taaatcattt atctagatat ctcaataaaa aatgatttct aagaaacttg tattttcgtg
    51901 ttattatttt tttgccctat caaaatttct aattcttcta gcgtaatatg attatgacac
    51961 ataatcgatt caattacttc aatacctatt tttttcgaga aagatccctt ttgttcactc
    52021 acaaaaatac tttcagaaga catatataag gaacgaagtt ttggaattag tatatcgtac
    52081 aaatagacca ccataaaatc gtccttgcaa ccattctcaa aaagaattaa atttgctgat
    52141 tgtgttacat atatcactga attacattca tggtcaattc caaaaacaat gtctacttca
    52201 ttttcttttc taattcgcgc agttacattt ttcaaacttt caccattact tataaatgct
    52261 ttagttactg taagctctga tgtttgttca gatattcctt ttataatatt ttgttcatca
    52321 gccacattcc ccataaatcc gccgattcct tttaagtaat aattctcacc taagagtcgc
    52381 ttgcattcac tgaattgata aaaaaaccag ttaaaaaaat cttctggaaa aataaattct
    52441 gatcgaactt tatttatatt tttagctcct attaatttta taactcgatt aatgttcact
    52501 tgctgcgatg aacatataac tgaataaatt gagttgttta attcaaaaaa acaactttaa
    52561 catatgcatc attaattcgt tattttttag gtagatattg atcattgtta tctctctttt
    52621 tttgcttccc atactccaac ttagaaattg ccgttataaa atttatttta aaggtgactt
    52681 tttcccctga agtatcggta gtaggaatac ttaatacctc agcttctgag gaggtatccc
    52741 aattatagtt atactttgca tcatcaccat aaagaacaat tccatttttt ttatcttgtg
    52801 ccgttagctt tactggttcg cttacttttc ttgttaatat cttggacttt acatcatcga
    52861 tattgcttat tttatcaagc ttaaataatt tacaaattaa tttcgtacta gtttttatat
    52921 ttgacgagag tgtaaaatat tttgtgtaaa tagaaaaaag gaagtccctt ctgtagaata
    52981 gagttaccac aacacattca cagaaaagag gacttccata tgaacgattt tactacagaa
    53041 attctaaaga ctctagcgaa caaaggcgat tt
//
LOCUS       DAFEZB010000010        52627 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFEZB010000010
VERSION     DAFEZB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.010"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            83..970
                     /locus_tag="LP859_000653"
     CDS             83..970
                     /locus_tag="LP859_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL3028629.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="LP859_000654"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="LP859_000654"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL3028630.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="LP859_000655"
     CDS             2272..2718
                     /locus_tag="LP859_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL3028631.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="LP859_000656"
     CDS             2991..4343
                     /locus_tag="LP859_000656"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL3028632.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="LP859_000657"
     CDS             4514..4801
                     /locus_tag="LP859_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028633.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="LP859_000658"
     CDS             5027..5470
                     /locus_tag="LP859_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3028634.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="LP859_000659"
     CDS             complement(5476..6660)
                     /locus_tag="LP859_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL3028635.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="LP859_000660"
     CDS             6928..7626
                     /locus_tag="LP859_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL3028636.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="LP859_000661"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="LP859_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL3028637.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="LP859_000662"
     CDS             complement(8436..8969)
                     /locus_tag="LP859_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028638.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="LP859_000663"
     CDS             complement(9266..11647)
                     /locus_tag="LP859_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL3028639.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LP859_000664"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LP859_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL3028640.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="LP859_000665"
     CDS             12348..12887
                     /locus_tag="LP859_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL3028641.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="LP859_000666"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="LP859_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL3028642.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="LP859_000667"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LP859_000667"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="LP859_000668"
     CDS             13994..15160
                     /locus_tag="LP859_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL3028643.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="LP859_000669"
     CDS             15206..16702
                     /locus_tag="LP859_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL3028644.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="LP859_000670"
     CDS             16783..17466
                     /locus_tag="LP859_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3028645.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="LP859_000671"
     CDS             18111..19301
                     /locus_tag="LP859_000671"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL3028646.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="LP859_000672"
     CDS             19403..20356
                     /locus_tag="LP859_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL3028647.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="LP859_000673"
     CDS             20425..21774
                     /locus_tag="LP859_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3028648.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="LP859_000674"
     CDS             complement(21957..22280)
                     /locus_tag="LP859_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028649.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="LP859_000675"
     CDS             22409..23572
                     /locus_tag="LP859_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL3028650.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="LP859_000676"
     CDS             23642..24991
                     /locus_tag="LP859_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL3028651.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="LP859_000677"
     CDS             complement(25036..25467)
                     /locus_tag="LP859_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3028652.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="LP859_000678"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="LP859_000678"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL3028653.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="LP859_000679"
     CDS             28697..29293
                     /locus_tag="LP859_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3028654.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="LP859_000680"
     CDS             29415..30563
                     /locus_tag="LP859_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL3028655.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="LP859_000681"
     CDS             complement(30601..31014)
                     /locus_tag="LP859_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL3028656.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="LP859_000682"
     CDS             31093..31524
                     /locus_tag="LP859_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL3028657.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="LP859_000683"
     CDS             complement(31623..31856)
                     /locus_tag="LP859_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL3028658.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="LP859_000684"
     CDS             32039..33709
                     /locus_tag="LP859_000684"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL3028659.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="LP859_000685"
     CDS             33856..34242
                     /locus_tag="LP859_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028660.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="LP859_000686"
     CDS             34229..34927
                     /locus_tag="LP859_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3028661.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="LP859_000687"
     CDS             34932..35396
                     /locus_tag="LP859_000687"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL3028662.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="LP859_000688"
     CDS             35393..36304
                     /locus_tag="LP859_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL3028663.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="LP859_000689"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="LP859_000689"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL3028664.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="LP859_000690"
     CDS             37092..38375
                     /locus_tag="LP859_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL3028665.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="LP859_000691"
     CDS             38388..39314
                     /locus_tag="LP859_000691"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL3028666.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="LP859_000692"
     CDS             39333..40613
                     /locus_tag="LP859_000692"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL3028667.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="LP859_000693"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="LP859_000693"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL3028668.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="LP859_000694"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="LP859_000694"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL3028669.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="LP859_000695"
     CDS             44938..45714
                     /locus_tag="LP859_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL3028670.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="LP859_000696"
     CDS             45711..46643
                     /locus_tag="LP859_000696"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL3028671.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="LP859_000697"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="LP859_000697"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL3028672.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="LP859_000698"
     CDS             47363..47992
                     /locus_tag="LP859_000698"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL3028673.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="LP859_000699"
     CDS             47980..48294
                     /locus_tag="LP859_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028674.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="LP859_000700"
     CDS             48332..48973
                     /locus_tag="LP859_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3028675.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49016..49708
                     /locus_tag="LP859_000701"
     CDS             49016..49708
                     /locus_tag="LP859_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL3028676.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="LP859_000702"
     CDS             49705..50607
                     /locus_tag="LP859_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3028677.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="LP859_000703"
     CDS             complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="LP859_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL3028678.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17216 a         9161 c        11301 g        14949 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggta
//
LOCUS       DAFEZB010000011        50578 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFEZB010000011
VERSION     DAFEZB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.011"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..1235)
                     /locus_tag="LP859_000704"
     CDS             complement(<1..1235)
                     /locus_tag="LP859_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL3028679.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="LP859_000705"
     CDS             complement(1228..2820)
                     /locus_tag="LP859_000705"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL3028680.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3330..4055)
                     /locus_tag="LP859_000706"
     CDS             complement(3330..4055)
                     /locus_tag="LP859_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028681.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4209..4535)
                     /locus_tag="LP859_000707"
     CDS             complement(4209..4535)
                     /locus_tag="LP859_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028682.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4572..4937)
                     /locus_tag="LP859_000708"
     CDS             complement(4572..4937)
                     /locus_tag="LP859_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL3028683.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4974..5189)
                     /locus_tag="LP859_000709"
     CDS             complement(4974..5189)
                     /locus_tag="LP859_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028684.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5318..5653
                     /locus_tag="LP859_000710"
     CDS             5318..5653
                     /locus_tag="LP859_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028685.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5729..7726)
                     /locus_tag="LP859_000711"
     CDS             complement(5729..7726)
                     /locus_tag="LP859_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028686.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7824..8099)
                     /locus_tag="LP859_000712"
     CDS             complement(7824..8099)
                     /locus_tag="LP859_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028687.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8331..9065
                     /locus_tag="LP859_000713"
     CDS             8331..9065
                     /locus_tag="LP859_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028688.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9104..10246
                     /locus_tag="LP859_000714"
     CDS             9104..10246
                     /locus_tag="LP859_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3028689.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(10874..11359)
                     /locus_tag="LP859_000715"
     CDS             complement(10874..11359)
                     /locus_tag="LP859_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBL3028690.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(11361..12797)
                     /locus_tag="LP859_000716"
     CDS             complement(11361..12797)
                     /locus_tag="LP859_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBL3028691.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(12830..13681)
                     /locus_tag="LP859_000717"
     CDS             complement(12830..13681)
                     /locus_tag="LP859_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBL3028692.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(13837..14082)
                     /locus_tag="LP859_000718"
     CDS             complement(13837..14082)
                     /locus_tag="LP859_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028693.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            14187..14960
                     /locus_tag="LP859_000719"
     CDS             14187..14960
                     /locus_tag="LP859_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028694.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(15013..15327)
                     /locus_tag="LP859_000720"
     CDS             complement(15013..15327)
                     /locus_tag="LP859_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028695.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(16846..17064)
                     /locus_tag="LP859_000721"
     CDS             complement(16846..17064)
                     /locus_tag="LP859_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028696.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(17079..19706)
                     /locus_tag="LP859_000722"
     CDS             complement(17079..19706)
                     /locus_tag="LP859_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028697.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(19693..19911)
                     /locus_tag="LP859_000723"
     CDS             complement(19693..19911)
                     /locus_tag="LP859_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028698.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(19908..20783)
                     /locus_tag="LP859_000724"
     CDS             complement(19908..20783)
                     /locus_tag="LP859_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028699.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(21048..21347)
                     /locus_tag="LP859_000725"
     CDS             complement(21048..21347)
                     /locus_tag="LP859_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028700.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            21549..22346
                     /locus_tag="LP859_000726"
     CDS             21549..22346
                     /locus_tag="LP859_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3028701.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            22397..23539
                     /locus_tag="LP859_000727"
     CDS             22397..23539
                     /locus_tag="LP859_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3028702.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(23637..25028)
                     /gene="sufB"
                     /locus_tag="LP859_000728"
     CDS             complement(23637..25028)
                     /gene="sufB"
                     /locus_tag="LP859_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL3028703.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25033..25503)
                     /locus_tag="LP859_000729"
     CDS             complement(25033..25503)
                     /locus_tag="LP859_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL3028704.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(25490..26725)
                     /locus_tag="LP859_000730"
     CDS             complement(25490..26725)
                     /locus_tag="LP859_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL3028705.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(26722..28008)
                     /gene="sufD"
                     /locus_tag="LP859_000731"
     CDS             complement(26722..28008)
                     /gene="sufD"
                     /locus_tag="LP859_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL3028706.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28021..28794)
                     /gene="sufC"
                     /locus_tag="LP859_000732"
     CDS             complement(28021..28794)
                     /gene="sufC"
                     /locus_tag="LP859_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL3028707.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(28991..29839)
                     /locus_tag="LP859_000733"
     CDS             complement(28991..29839)
                     /locus_tag="LP859_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL3028708.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(29863..30549)
                     /locus_tag="LP859_000734"
     CDS             complement(29863..30549)
                     /locus_tag="LP859_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028709.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(30542..31576)
                     /locus_tag="LP859_000735"
     CDS             complement(30542..31576)
                     /locus_tag="LP859_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028710.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(31639..31895)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(31971..32309)
                     /locus_tag="LP859_000736"
     CDS             complement(31971..32309)
                     /locus_tag="LP859_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL3028711.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(32311..32667)
                     /locus_tag="LP859_000737"
     CDS             complement(32311..32667)
                     /locus_tag="LP859_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL3028712.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(32673..33860)
                     /locus_tag="LP859_000738"
     CDS             complement(32673..33860)
                     /locus_tag="LP859_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL3028713.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(34051..34896)
                     /locus_tag="LP859_000739"
     CDS             complement(34051..34896)
                     /locus_tag="LP859_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3028714.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35130..36095
                     /locus_tag="LP859_000740"
     CDS             35130..36095
                     /locus_tag="LP859_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL3028715.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            36472..37515
                     /locus_tag="LP859_000741"
     CDS             36472..37515
                     /locus_tag="LP859_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL3028716.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(37577..38041)
                     /locus_tag="LP859_000742"
     CDS             complement(37577..38041)
                     /locus_tag="LP859_000742"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL3028717.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            38287..39234
                     /locus_tag="LP859_000743"
     CDS             38287..39234
                     /locus_tag="LP859_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3028718.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(39445..41517)
                     /locus_tag="LP859_000744"
     CDS             complement(39445..41517)
                     /locus_tag="LP859_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3028719.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(41450..41827)
                     /locus_tag="LP859_000745"
     CDS             complement(41450..41827)
                     /locus_tag="LP859_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL3028720.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(41956..42534)
                     /locus_tag="LP859_000746"
     CDS             complement(41956..42534)
                     /locus_tag="LP859_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3028721.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            42666..43958
                     /locus_tag="LP859_000747"
     CDS             42666..43958
                     /locus_tag="LP859_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL3028722.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(44018..45136)
                     /locus_tag="LP859_000748"
     CDS             complement(44018..45136)
                     /locus_tag="LP859_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL3028723.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(45150..46571)
                     /gene="arcD"
                     /locus_tag="LP859_000749"
     CDS             complement(45150..46571)
                     /gene="arcD"
                     /locus_tag="LP859_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL3028724.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(46817..47938)
                     /gene="mnmA"
                     /locus_tag="LP859_000750"
     CDS             complement(46817..47938)
                     /gene="mnmA"
                     /locus_tag="LP859_000750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL3028725.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            48342..48941
                     /locus_tag="LP859_000751"
     CDS             48342..48941
                     /locus_tag="LP859_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBL3028726.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(49079..50365)
                     /gene="efmA"
                     /locus_tag="LP859_000752"
     CDS             complement(49079..50365)
                     /gene="efmA"
                     /locus_tag="LP859_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL3028727.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT        14939 a        10156 c         8235 g        17248 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
     3001 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
     3061 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
     3121 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
     3181 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
     3241 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
     3301 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
     3361 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
     3421 aaccgaagtg gaatatcttt accaactttc tctgtcactg cattactgat acctgttaga
     3481 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     3541 gtgattcctt tatcttcatt tacaagtttc caagtaggtg gtatagctgt gtatgtttct
     3601 acaaatgaat taagtttttc attaagtgac ttctctttta tatttccatc taaaaactct
     3661 gtatatatgg cttgagctat actaaaacaa agctggcctt gttctaataa ttttacatca
     3721 ttagttagta aagctgtgtt cacgttatca atcgcttcaa gaagagtatc tttggtgtcc
     3781 ctaacagctt taggtgtgcc ttctggaatt tccctacttg atacctcgtc cattttatct
     3841 tccataaacc ccttaaaatc atatctttct cccaaatcca tagaagtttt tgagaaagct
     3901 ttcatttctg agtctatttc ttcaataata tttaacatgt ctgatacata ttgtttaggc
     3961 tgattcgttt ttaatctata tgtggtaaat atctcaattc ctccaattat caataaaatt
     4021 gccaaacaaa tccataataa ttttttcttt ttcatttttc ctcctataca ctgctgttat
     4081 aacagtgact aatgtaaaga ttttcccagc aatcatcaga ctattattta cagggaatca
     4141 aaaaaactcc ttagtaagct tacaccttta ggagttcctg tttgaacggt atgcataata
     4201 catttaaatc aatttaacat taacacctca gtatatggta tctctacata tacacggtct
     4261 ttttttggat agtactcgac atttatattg atagcacttc cattgtctaa cgtagcttta
     4321 gcccggtacg tgttatcacc attatcatga gtgatcgtca catcatcaca tgtccgattt
     4381 aagttatact gttcttgtaa aatctgagtt accaatggta cagatgactt ctctagtcca
     4441 gatgaacccg tcattcttga aaaaattata cctataatag ccaatacaac gatacctata
     4501 atgacaaact tcttattcat atttacttta ttcaactttt tatctcctat ctccaaaatg
     4561 attttctctc atcagtaact cattgacatc cccaatagta caccaaagat ggctccagct
     4621 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tctctcgata taagtaaccc
     4681 ataataacag caacagcacc aaataccaca ggaataaatg ctaatgatac taccatagaa
     4741 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     4801 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     4861 gtttcttgta atgctccgca aaactggcaa tacttacttt catttgttat ttctttacca
     4921 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagctaactg
     4981 tttactttat taaatacatc agaaaacagc tcatttattg gtacacttaa tatctcagaa
     5041 agcattttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     5101 tgctgtgtaa cattcatttt tttggctaac tcagtttggg aaagtccttt ttcttttctg
     5161 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     5221 aatatgatgt atttcaactc ataaaatata taaatacttc aaatgtcatt acaaatacaa
     5281 caacttgttg tactctactg agaagggggg tgtattaatg aaacagaagc aatatgcaaa
     5341 ggtcattaga gaaaagcgaa aagcactcgg gtggacacaa aaagaattag ctaaaaaaat
     5401 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagaac ctaacctcga
     5461 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     5521 tgacatagat tatgaggaag agtttttggc tctctatcgc tctcttagta ctgaagatgc
     5581 aattcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     5641 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     5701 ataagattaa gagctatgga taacctgtct agtttaatat aattgggtct cttctattcc
     5761 ttactctccg tccatcatct cttgttacac ttgtaaattg ataaatagga actaattcaa
     5821 ctcctgataa tatcaattct tttattactt catctatccc tgcccctttc gctagatata
     5881 ctaaaaaact actgcttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     5941 caagagtgaa tccttgttta acaaagtagc ctctgttttc tattaaagca tttttcacgc
     6001 tgttatccct gacatttcgt aggcgttcac aaacagtgtt aataagttct ttttctgtca
     6061 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     6121 attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     6181 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaaatt
     6241 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     6301 cgtctaatcg atagacattg cttctcttat gtttaaagtt tcctaacgct ttctcttcct
     6361 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     6421 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     6481 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     6541 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     6601 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     6661 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     6721 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     6781 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     6841 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     6901 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     6961 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     7021 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     7081 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     7141 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     7201 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     7261 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     7321 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     7381 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     7441 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     7501 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     7561 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     7621 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     7681 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcct
     7741 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     7801 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     7861 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     7921 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     7981 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     8041 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     8101 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
     8161 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
     8221 catcgacata cacataatac ctcgataaaa aaggggtgtc aatagttttg taaaatttat
     8281 ttacaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
     8341 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
     8401 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
     8461 ttgacaaaaa gactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
     8521 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
     8581 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
     8641 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
     8701 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
     8761 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
     8821 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
     8881 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
     8941 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
     9001 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
     9061 tctagtggta agtactcctt gtaaactaag aggagatgat ttcatggcaa aaatatcaaa
     9121 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
     9181 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
     9241 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
     9301 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
     9361 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
     9421 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
     9481 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
     9541 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
     9601 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
     9661 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
     9721 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
     9781 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
     9841 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
     9901 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
     9961 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
    10021 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
    10081 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
    10141 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
    10201 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
    10261 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat atatcaaggg
    10321 ttctagctcc tataacaggt ttacccaaca ctgccttcca atgactcttt tgggaatcag
    10381 aggaaagaat caggctttat agagatattt catctaacaa attactttag actgacagta
    10441 tatatcagca ggtactgtca tcgggaacgt agccaatatc gtatccaaaa cagcaggtag
    10501 agaagctatt tgaggattca agttattcta tttcaaccat gaagttaagt tcattaaaaa
    10561 taaagatagt tggtatttca ataatttttg tatagagtta atcactttat tgatgctaat
    10621 tcatcttgat tggtattata atcgtattca agttgcgtgg cgtgcttttg ctagaaaata
    10681 tataactgtt ctttataaag aaatataagg attaaacatt ttttatcata gagttttact
    10741 tatctataga attatgtctg ttcctaattc agtttgatga attaactgat aaaaaaatat
    10801 ctactattta aagatacagg gataagtaag taaaaaaata acatgtcatt agatagtaga
    10861 cagttccaac tctttattta ccttttttgt atgctgcact gtcaatccaa gttcctaaat
    10921 tgttgtatcc atcatcactt atataatcat attggccatc aataatttcg tcaaaccaca
    10981 aatattctcc atctggtttt tgcccaatta ttttaattac tgattgacta gtagttgtca
    11041 tctggttaaa atcttttatt ttacctatat taacggccaa aatagcattt cctcgcttat
    11101 aactctcctg gatttcatat ttaacatatt ctcgatcaag agtttcttta cctatcagca
    11161 ccacagtcac tgatgttcca attaattgat tatctatcca gttttttata gcaatatctc
    11221 cattacgttt aactttctca aattcagcct tatctacaaa accagcttcc tttgttcctc
    11281 gcaaagtcca gctgtttcta actaccattg ctcttgttac atctgcataa tggaaactaa
    11341 aaaaaaccct tctcaccata ttaatcctcc tgaatactat ttattaaact gacaatttgt
    11401 tcaacttttt tatctacttc tccttcagta tctaattttt ctagcgcttg aaatttactt
    11461 tttaaggaat catcaaaata atcactgaaa ttctcttgaa tcttatcaaa caaaacctta
    11521 cttgcacctt tggtaaatcc aatcggaact actatatttt cattttgaac agataattca
    11581 aattcttcaa tcattccatc tgctgtaact atttcacctg attcaagttt gtttccaaaa
    11641 acaaaaatac taatcccaga ctctgcaata atgttttctc gatatcttct ccactcatga
    11701 ggcatatcaa ggggaagtgg tcttaaagaa agtacgtctt gtatgttatt ttttcttaaa
    11761 tttcttctct gctttagaac tcctgaaaca atactattac caattccaag tccgtaacct
    11821 gatactattc tataaccgga ttcagaaagc ctatatgcta atttctcgac aaattttaat
    11881 cctttcgatt cagatgtaaa tggagtataa tcaacagcac ttcccgaaat aaaaatagtt
    11941 tttctcaaat acttttttct aatacttttc aaaatatcag taatctcaga ataatcatct
    12001 acttcaacaa cgtttatcga aaatctggac aaatcattga tttgtagtct tctttttgtc
    12061 agttcataat tatatctctc ttgatcaaaa aaatcctcag aatcgacaaa attttccttt
    12121 aatatttttc tcattataca ataatgaggt ctttggtttt ctctcaaaac ccatcttata
    12181 tctgataaaa ttctttctaa attagggtct gtaaaactaa atcctaaaaa gagaaaagtt
    12241 tttgttaaca aatctccttt taaagtttct ttaaataacg cgtacgaatc atcatcatat
    12301 ttttcataat catctcttgt tattactgtc gaactgacat catctatatc accatgcatt
    12361 ttataaacaa ttgtatccac atttggatta gttgttgcta actgagtata atttttctta
    12421 acctgagggt ttttataagc ttctcggatt gatttttcta ttagatgatc ataattagtt
    12481 gtccaataag tttcaatagg taaacttgct agtattcgat gattttcaga tatttctaat
    12541 tcttcaccaa aattatccat aagtatctga ttcaaaattc ctcttgatcc accattttca
    12601 tttttataat attgagccag agtaactaag tctgtttctt ttttagaatc caacccgatt
    12661 tctgttgcag gatcattaag tagttccggc caacttacat atccttgtga catcgataat
    12721 cctgcacctg caaatattgc tgcatttcct ttttcaatag cttcagagaa cctctttatt
    12781 aaaactttct tatccattct acccggttcc tttcgattca gtaaattgat tagagattag
    12841 tgaaaatggt gtttttatat aaaatatcta atgtattact tcctttagtg cattccacag
    12901 catctaaagc ccattttttc aaatcagctt ttcgtaaata agttaattca cttaaattta
    12961 aagaatattt tatttgcaga ttttcttgcg aagaatagtg ctttataatg cttctaacat
    13021 cctcggtgct catttgagga ttactttcca atcgtggatt ctttttttcc actattgaag
    13081 ctgtcgtcag ttcgctatat agccatggcg aatacgtaaa tttctgttca ttagtgattt
    13141 cattgctcaa actaattgaa ttaggtgtat taagaaaaaa cagacattct gtactatcaa
    13201 tcatattatt taaagctgta gaaagcatca tatgaacatg actagttgtt acatttcttt
    13261 tatcataatc atatgttttt gtttgttcct tatagcaata tttttggtct atttttttta
    13321 ataaatcaga actgtatccc caaaccgaag aatcaataaa tgcagtaaga ccaaaatttt
    13381 caaatagcca tgatgcaaaa cgtatagcta atttttcatc agcatgagag tgagatataa
    13441 aaatatctgc ttttatagtt ggaaaccaat ctttaataat ttcgtctgca tctagaattt
    13501 tgctgtcctt agaataataa tcaactaaat cattttcact ttcaaataga ttttctattc
    13561 tcttgttaat agatttttgg ttgtgatcta tttgatttac tacatgtgta tatgattgcg
    13621 agcttgagga gaatgcattt aagtcagcat ttgtaaaatc caattcatat gccttataca
    13681 aggatatccc tccattcttg tattttgatc cacaatttat taggcaacat aacagtatac
    13741 cctatcgtta catatcccat tcattattat accagtattt cttcgtgaca cataagggtg
    13801 taacataata atttcaaacg aaatgactac aagtagtcat tgaagcaaat cttcataaga
    13861 cacctgtaga atatctttga tggctttcaa ttgatccaac ttaatgtgtt ggcggccacc
    13921 ttcaatctta accaacgttt ccctagttat agcaatttta cgaagctgta ggtctctaat
    13981 cagctcggtt tgaccaattc ctttttctaa tcggactcgt ctaatattgc taccgattac
    14041 attttcttta tcaataattt tttcctcttt caagaatagc acaccttttg gactaaaatt
    14101 agtccttttt cttattaatc ttaaagattc ggtatgctac aatgggacta gtattagtcc
    14161 atattcaatt ttaaggagga atttttttga agaaatttgc aatcattcta acttgcgctt
    14221 tactcatcgg aactataagc gggtgtacca cttctgacac aagttctaca agtacggaaa
    14281 ctagtacaac aacctcagaa aagaattacc caatacattt ttttccggtt ccagatggat
    14341 ttgaagctta ttatcatgat aaaaaactaa atgttccttt atcagaaact gaatctactg
    14401 aaaagatttt gccaattgaa attgaaagaa atcaagacga tataaaacta aaagctgatc
    14461 ttcccttact gaacagcaca caaattgata gacatgaaaa tattaaagtt aatattgaaa
    14521 aaggagcgtt agcacaactt tatactgaaa aagttgaaca attttttgag gctggtgcca
    14581 ataaagactt ttcaaaaata agcgattatt cggataactt ttttaatgag acccaagatg
    14641 aaatcacgaa ctcaactttt atggctgaag atatgtacaa tatgaagttg aaaaaaataa
    14701 agtcgtatga ttatccatta aatctaacat taaataaagg gatactctct tttgtattag
    14761 caggttacgt tgactatgaa tatacagcag attatttgga gacgcccctt caacacgata
    14821 ataacgatat taattttaga tttatctaca attcaaaaag taaaacatgg ttagttgatc
    14881 aattgagcac tataaatacg ggatttcatg aattacctaa gcctgacaat gaaactgctt
    14941 tggtcattaa aacattctaa aaaagaaacg ttgaaggaat aattattctt tcaacgtttc
    15001 ttttattgtt tttcagttta cggtcttacc tgagcgaata atctgatttc taaatgattg
    15061 gaaaacttct tgtggttgat aataatctgt atctatttca aatgtttctg agaataattc
    15121 attgaatttt tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat
    15181 ttctgcctct aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg
    15241 aacaaaaaaa tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag
    15301 cggaacaact ttattttcat cagccattct tccaatttcc tcactttcat tttcaattgt
    15361 ccattgttgc ggacgtatca gattttctct atcatttttc agtgtataga tcacttgtaa
    15421 tacacaacaa tgctttgtaa acttattttt ttagttagtc gaatcttttt aaatgtgcgt
    15481 ttcataccat gcgcttgata tattttctcc tattttttaa atcgtttatc acaactttta
    15541 tcgttttttc ttctgaaaaa aatcttgttt acatcgttac atagtttcca aaaagataca
    15601 ttactagtag aaacaacaag attaatttta cttcccactg aaatgggaag tatattttaa
    15661 atattttttc tccttcaact tacactataa agtatcttaa tcacacgata tgctagccac
    15721 taaattcatc atatttgatt ccttttttaa gataatgtag catcttttta tccaataacg
    15781 caattgtttt taaggataaa tttcttaaga tggtacattt caaaatggca tttgacatat
    15841 gaaacaaaaa agaaagaaaa gagcataagg gaaaactgat agcaccttcc tacaggaagg
    15901 tggctaagtt ggctgtgcca actggttttc tttcaaaatc atttcataat ttttgatatc
    15961 ataaaaaaat ccattttgga cctattttcg gtcataattt agtacctact tttggtcata
    16021 gtttcgtccg tagttttggt catagtgact aaggcttttc tgttcacaaa attggtttaa
    16081 atttgctttc tttaacactt catttatatc atagcaatta taatgtttac acaacatcat
    16141 tcctaatttt aacggttgtt cctctttata ttttttaagt tctacatttt tttgcgttta
    16201 attacttttc tcttttcttt atattccgct ccaactttct ctttaatagt gataatagaa
    16261 ttacatattt ttgttaaact tccttaaaaa aattggatct cgattactca aatttgcttg
    16321 caggcttttc taattgcttt gttttttctt cctaattatt tgattttatt tccattttct
    16381 tctagtgaaa atttattatt tttaataatc gtacgcgtag aactgatttc attttttgta
    16441 tctgtttgat tatctaacgt ttgaaattca aagttttgat tggctttatc aatatctttt
    16501 tatactactt tatttgcaag atatattttc ttgcttaatt tggtcatagt ttcattcgtc
    16561 acttcggtga tattttttta atttgcaaat aaaaagagtt aaaccgatat acattaggtt
    16621 caactctttt gcaggtttca tgggtgtact atcactttta atctgataga tgtaaacctc
    16681 tctttatctt tttgttactt caattttcaa caatagctcc tttataggtt gtcaatattg
    16741 ctctacgtat tctggctttt taacccatta tttctctaaa gtggaagaga ataatcgctt
    16801 aatccccact aattccttgt cgtaaattca tgtcaaatac aatttttaaa agccatttaa
    16861 ttcatggatt atctcttcac ttttttctag ataacaatat ttcaagttgt tttttatctc
    16921 atgtttcatc tttaattctt cttgaatcat cagttcaata taattagtga ccgtgatttt
    16981 ttcatttgtt gcgttcttta ctgattcaca taattcgtca agttctaaga aatcatgcgt
    17041 actaatcttt agaattaatt tcatgacaat tccccctttt atttttttaa tagattatcg
    17101 aaaagcggat taggatcatt aagtgtatct ctgaataact cgttgtcttc ataggtaaat
    17161 cttcgtcttg gttttgatcc cttatcattt tcacggattt cactatcaat aatcactgtt
    17221 tcatgatttg aatcaaattt tttcgctgac tttctgtctt caacgatatt gattttttgt
    17281 gttttttctt tcatttctcc actatctttt tgaacgttct cccgaacatc tactgttttg
    17341 aatccttgac tacttgtaga actttgtaca ttctctctta catccattga tttaaaccct
    17401 tgactacttg tagaattttg tacattctct cttacatcca ttgatttaaa cccttgacta
    17461 cttgtagaat tttgtacatt ttctcgaaca tcaattgtct tagtaccttg accatttgaa
    17521 gagctttgta aattctctct tacatcaaca ttccgtccga catgaccatt caatgaacct
    17581 tgaatatttt ctcggacatc tactgatcgg ccaccttgac tacttgtaga attttgtaga
    17641 ttttctttga ccaatttttc taatccggta tttgagtttt tagcttcttg gattctttct
    17701 ttggtcaacg cttctttttc gtccgtatta gctcctgtac gcccttctac acgtttcctt
    17761 aacatttcct cacgattcat tgcttcaggc gtgtatttag cttcttgtcg cttctgatta
    17821 attaattctt cagcagactt agcttgaccg tgcatagcat ttttacgttc ttcaatatct
    17881 tgttgaactt tttgctgaat tgcacttaca ggattattat tcgattcagc attatttttc
    17941 attgcttcac gcatacgttc agaactagac ataattctat cggcattttc aaccttagca
    18001 ccatcttttc tatccgaaag acgtgttggc atggaatcag catattttcc agtttcatct
    18061 tctttatttt tagctaacat aggtttgcta tttttattaa tagcggacac tgtaccatca
    18121 tctaaacctt ctttgaactt attcttggta tcattagcca tatcacgcat actcttaaat
    18181 ggttttattg ctctttcact agccattgta gctccatctg aagctaaacc tgataatcca
    18241 cgttcatggg catatcccaa agctcttcca gttttgccag ctttttttga tagagtttcg
    18301 ccgaaattct tttctggttt tcgatcttct ccttctccac tgtctgatcc atttccattt
    18361 ccgcttcctt tagcacgatt aaatccagtt gatccttttc taaacagcat tgcgcccata
    18421 ccaaaagcag aagcaagttg cccatagcct tctttgagtc caatatcaac accaaagtat
    18481 ctcatgactg ttccactgcc ttttatcagc acaaacacag cacagatata agcaattgtt
    18541 tttataaaaa tatttgtgga gataccttgt ccagcattta aaaaattgat aaacatagca
    18601 aacatagata gtccaaaccc ttgaaatcca actgttaagt aacattgtag aatatctgat
    18661 agaacaactt ttgaacgttg tcctgtttca atatctgtcg caaaaacaag aacacctaac
    18721 acacgtttaa atgcaagttc taatattgct gtaatgatca caaacgcgct gaacacatag
    18781 gcgattgcca acgctgtcaa cccaatcgaa atcccccaaa gatttgcttg atatcgataa
    18841 taacctgatt tcaagctatc agaaaatgtt gataagaagt tatcagaaaa ctttgtggca
    18901 acaaattcat tattagaatt ttcaactaat tcatagcgaa gattatccgc agctggattt
    18961 tctttttcca atttatcaat cactttatcc gtcaacactt gtgttaaatc tgtctgttga
    19021 aaattttttc gtgttaactt atttcgatcc tccgttttat caatgcttga gtattgatct
    19081 gttttattaa tatatgccag atctgttact ccttgtttta tcaatgacca agcgactccg
    19141 tcatctgaat ttgtaatcgt agtagcatca ttgtaaaata ttttggcaaa ttgaataccg
    19201 ctagaaatca ttgttggcat taaaagaatt aacgctacag acatcactat attcattcca
    19261 acagatctta aatctattgt tccttttcca gtaatcatct tgtagccaac aaacatcaat
    19321 gaaaggatca ttaatccaac gacaattgta tttatgactg attgataaac attatttagc
    19381 cctgtacttt caatgaaatt gaacaaatcc aacacatctg gaatcatatt ttgaatcgaa
    19441 tcagtcacag taaacatgcc ttttaccaca gcccatccaa gccaaatccc gaactgacta
    19501 aggaaatctc cattgtattt ttttaggtaa ggtctataaa gagaattatt caaaaactct
    19561 gtttgacttt ttgtgtcatc aaaccaattg aaaatgctag cttcaacaat attacttgta
    19621 aaaacaacgc taatcattaa aacaagtaca ctgagtgcta accagtttat ttttctacta
    19681 acgatttttt ctttaattgc tttcatagtt ttctccttca gcccattttc ttaggctctc
    19741 agagagctgc tgagccattt tttgattaat tatgatatga gttacacaat tgtcattttc
    19801 agctcttaaa tcgagaacta ctgtgttgaa gcagatactc tcgattaatt cgagagtatc
    19861 tgtattttga tcaagacacc acatttgatc tattaagcga tttttaatca tgattcttct
    19921 cctttcaacc aattatgcat agtaatttca ggtaactctt tgatttcgaa agcttttttg
    19981 aactgatcta taggctctac attttctaaa aattgacttt tatatctttt ctgaagtcca
    20041 ttcaaaaaat atgttacata atcgtataac ccttcttcag tttttctttg aaaaaagcat
    20101 gcttcatgtt gttttataat acactcagca ataagatact gagtattttc agataaatat
    20161 tcaatgattt ttctgatatc ttctgtatct acattagcgt ttctcaaata gtgatacact
    20221 tgaaataatt ttcgaccatc ttcgttattt gcgtcataaa gaacactaat cttactgtaa
    20281 atcctcttta tattaatatc ctcttcttcc ataacgagag tagtctttga tatgttctgt
    20341 gaagtagtct ttgtatacgt atatacaaat ttgtacatac ctctaccttt ttttaaagag
    20401 acatatctct ttttttcgat acccctctga cgaattttcg gcatatactc ctttttttcg
    20461 caatccaaag taggaaaagt aatttctatc aatttatgta catttaaaac aatgtacata
    20521 acatttgata atgtaactgc tcttttctca taataaatat ttctaaaaac ccttttgata
    20581 atcccctttt tctctaagta cacaattgca tcttttatag ctcgttctga taatcctgtt
    20641 tttttgctta attgcttata tgatttttgc aaaatatctt caagaaactt tttttgataa
    20701 ccaagtagct gaccatcatc gttaaaaatt tttttaggtc tataccaata ggtaatttct
    20761 gctaagataa ctattgcatt caaatagggc ttctcaacac cttttttcgt tttttttctt
    20821 aattgatcgt accatgtgtg tggattacat ttccgtcaaa atttatttct ctaagtttgt
    20881 caactacaac atttccagaa gaaaacacct tttcaccttc ttttttgata taatagctat
    20941 aaataaagtt ttctcagcac tttattattg agtgttcccc cagtctgcaa acttttggga
    21001 acactttttt tcttttttta aagcattttt ttataatttc tacttcatca tcttccaata
    21061 ttttcccctc ttttcattcg agcaaacata aactcattaa ctgccttttt cgaataaaat
    21121 tttcttgccc ctaattcaac tacgggcaat ccctcatttc tcacagcatt ttttaaaaag
    21181 ttttcactca catttaagta ttccgccatt tctttttgat tgagatattc ccttgtcatc
    21241 cccgcatttt cttgagcctt ttcaatagct tttgtagcaa tttgataaaa ctgctttaga
    21301 aacatttcct cctgttcttc tgaaatcatt aattttattg cttccacaaa aacctctcct
    21361 ttttaattaa ttattctata actcctctac ctttataagt acaaataaat acaaattgcg
    21421 taaaaaaatg tatttatttg tatttataag aataaagtat catactccag ataaaaagta
    21481 aatactttta aatactttta ttttataaat atgatatact atctttaaaa ataaaaagga
    21541 gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa ttacgccttt
    21601 catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca cgtgcgacca
    21661 tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa ttattatcaa
    21721 ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa tatataactt
    21781 cataccttat cgatataggg tatgagctat atattaagga cttcccagaa atacctcaca
    21841 aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgctagat aaagactatg
    21901 agttattgaa tcctataata aaaaatatat ttacaaaata ttactcaaaa gattttgaaa
    21961 tttatctaaa tgagatactt gctaataata tcaatcctct gattgaagag tacgctaacc
    22021 aaagacactc tatctctaaa gagctatttt tcaaaagagc caacagtaaa cttcaagaca
    22081 agataagaaa tggtgatttc aaatacggtg aaaaagaaag aatgattgat tctttaaaac
    22141 agctattaga cgaaatggac ttagcctata aagatatttc aaagtattca tctgtcgagg
    22201 atttctttta taaaattact aaaaaccaat ttgaaacaga aaaatttatt tcagatctta
    22261 gtaaaaaata caatttccca tatgagaaaa atagtataat cgcaaaattt ttaactgata
    22321 atcacgataa atttaaaaat aattgattct tcaaataact tctctacctg caataatgaa
    22381 gtaaggagag aatcaaatgg caacattttt tagttataaa ttacaaaatg gcgaaaaata
    22441 ttggggattt gaaacttatt tagggaaaga tcccaagact ggaaaagata tacgtacaaa
    22501 gcgtagaaga ttcaaatcaa aagctgaggc gcaaacagca cttaatagac tacttgtaga
    22561 ctttaaagaa aaacaagagt ttaaaatcaa tgttacaact tttcaagagt tgtatgaatt
    22621 atggattgat aactacagaa caagagtaaa accgtcttct gttgccgttg ctcaacgttt
    22681 ttgcaaaaac catatattac ctgcttttgg ggaattacga ttggataaaa taagtgttag
    22741 ctattgccaa aaacaggtga atgaatggca caaaacatta aaacaatatg gtttcttgag
    22801 aaaaacaact gctcaaatat ttaaatttgg agtagcaatg gaagtctgta aggataatcc
    22861 tatgtctaaa acattgcttc caagaaaaat tgaagaagaa caacccttga agttttacac
    22921 gaaagatcaa ttacaactgt ttttacagaa tactaaagaa aataattcag ttaaactgta
    22981 cacttttttc cgtttattag cttatacagg aatgcgtaaa agcgaagcat tggcgcttca
    23041 atgggaagat atagactatt tcaataaaac aataacaatt ggtaaaacta tcgctcaaga
    23101 cgagtttaat caagtagttc tacaagtacc taaaactaaa aattcatcaa gaacgattca
    23161 gcttgatgat tttacactta aacaactgcg tatttggcaa caagaacaaa tgaaaataat
    23221 gatcttatat ggctataaca cgaattcacc aaaacagttt cttttcacta caaacacaaa
    23281 caagttgtac tacccccaag tagtaaacga ttggttagac tggatttaca aaaagacacc
    23341 gatggagcct caaattactc cacatggatt taggcatacc cactgtagct tattgtttga
    23401 aagtggtgcg tcgataaaag aagtacagga acgattaggt cataaagata ttaaaactac
    23461 catgaatatc tatgctcatg tcacaccaca atctgttaag aaaactggtg accgatttgc
    23521 aaaatttatg ggcgaataat tccaaacgta gccaaaaacg tagccaacca ttaaaataca
    23581 aataaaaaaa cgctcgaaac gctttaatat caacgtttcg agcaatgaaa tagttattaa
    23641 cctacagacc cttccatctc ataactgatc aatcgattga gttcgactgc atattccatt
    23701 ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt tgcttcagat
    23761 tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt tgagactttg
    23821 gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat cgtatcggat
    23881 ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga acccgcactg
    23941 tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt tgaaattgat
    24001 ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc acccgtatct
    24061 tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc accttttcca
    24121 tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat
    24181 tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta cacattgtct
    24241 gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg
    24301 atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc gacatagtgg
    24361 atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt gttttccgca
    24421 ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg cacgtaaata
    24481 aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc ggttggcggt
    24541 acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc agagtcagtg
    24601 tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta cacgacttct
    24661 gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg tataccaatc
    24721 ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc gggtttgtca
    24781 cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat
    24841 gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc aagcatccat
    24901 tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa gccttctcct
    24961 gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc taattctggt
    25021 acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt
    25081 ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc gagcgggaaa
    25141 tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt tgacttcttt
    25201 tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta ttgattttcc
    25261 gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc cactgccgtc
    25321 aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga tcacatctcc
    25381 acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg
    25441 cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt tagatagtcc
    25501 catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg
    25561 ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg tacttcaata
    25621 gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct aaagctgtcg
    25681 cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg
    25741 ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc aacacataag
    25801 cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc aaataatcga
    25861 ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg aatttccaag
    25921 gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt
    25981 caacaggttc catttcttct aaccattttc gtttaccata taaaacacca atacctgttg
    26041 gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa
    26101 tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg ttttcatgga
    26161 caagttcagc taattcttga acaggattga agacacctaa gacattggat acatgagcaa
    26221 tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata tccaggaagc
    26281 cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc aactgctgcc
    26341 acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct cctgctttga
    26401 tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa
    26461 acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct cttgcttgtt
    26521 cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca ttggcgttat
    26581 cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg
    26641 cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata ggaaaatcat
    26701 tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt cgactaattc
    26761 tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc ctcgaatcac
    26821 taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca tttcttctgg
    26881 atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat cgatcaaaag
    26941 aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac tttcttgttg
    27001 ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg tcaatgttcc
    27061 tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg ctttattggt
    27121 tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga ttttgacttc
    27181 tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt
    27241 catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac ggcgattcat
    27301 ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt ttgaaccagc
    27361 tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt ctttggttgt
    27421 ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca acacatgctt
    27481 gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg gttcatcgat
    27541 cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta catggagtgc
    27601 agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt ggactagttc
    27661 tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt ctgctaattc
    27721 cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag gattgtcttt
    27781 cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc
    27841 aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca gttcatctgc
    27901 tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt tacttaaaga
    27961 aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc ttgctccccc
    28021 ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag cgtagccttc
    28081 tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc cttccatcat
    28141 gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg tgatgatcaa
    28201 tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt tcaaagcatc
    28261 gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta acatcaacaa
    28321 ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca gataacgttc
    28381 agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt tcaaaaatgc
    28441 cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac gcatgaattc
    28501 cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata agccaagtcg
    28561 cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa tttcgccttc
    28621 tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt ttcctgtgcc
    28681 gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta cgcctttaag
    28741 gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag acatgcatta
    28801 tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg aagcgcaatg
    28861 accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg atttctaaat
    28921 tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct catctttttt
    28981 gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc catttgtctg
    29041 tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg attttgtcac
    29101 tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg tccttttcaa
    29161 gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt gcgttgattg
    29221 caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg tcgatcgtat
    29281 ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct gtttccagat
    29341 ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg atgcgtcccc
    29401 agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg gaaacatctt
    29461 tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca ttgacaaagt
    29521 catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag taattggcat
    29581 caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat ttgacgattt
    29641 caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg
    29701 gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc
    29761 tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct gctgcaaatc
    29821 caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt tgtctgtttt
    29881 cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa taattaaagt
    29941 cgcaacgaat gtcactgtta gattaccacg ctggtagcct tcctgccaag caagacctcc
    30001 aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg agatcgttgt
    30061 aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct tccagatgat
    30121 ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc tgacttcacg
    30181 aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa gagctggaat
    30241 ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa tgatcaaaac
    30301 catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata cgattccata
    30361 aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca atcccaaaac
    30421 taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg tatcaaggat
    30481 agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt ctgaaaagct
    30541 tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac acttctgctt
    30601 gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag gaaccttctt
    30661 tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt cgaatcgctt
    30721 gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc aaacggccgc
    30781 ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct ggttcgatgt
    30841 cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca gtcaatacgt
    30901 ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag attttgcgga
    30961 tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta ttgatcgcta
    31021 acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta gcctcatcac
    31081 aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc
    31141 cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact aaacgcaaca
    31201 gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc agcggcagct
    31261 ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag atcataccga
    31321 ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct tgatcgttta
    31381 cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact aatgtacttt
    31441 ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg tttagtgaaa
    31501 cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg acattcgtta
    31561 gttggattaa tgacatgcca ttcacatctt tctcttataa tttttcaaac aaaaaagcct
    31621 tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac caccttgatt
    31681 taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct ttccgtatac
    31741 tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta ctctgctcta
    31801 agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc tcttcaaacg
    31861 tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt gcttatgatt
    31921 atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt ttataattcg
    31981 tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat ttcatctgaa
    32041 tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt
    32101 agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag aggttcatct
    32161 ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc aagttcttct
    32221 tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat ccaaaggttg
    32281 tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat aaattttttc
    32341 attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggtcgct tgatcagcat
    32401 accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta cttgatcttt
    32461 cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga ttggtacttg
    32521 actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga tcatatcaac
    32581 ggtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc atgttgaaca
    32641 tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct tgcaggtgct
    32701 tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc taaaatcgag
    32761 gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa tccgatattt
    32821 gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct tgcgatatag
    32881 gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat taaaataaaa
    32941 tacaagaaaa tgacgaatgc accaccgata aagccgaagt tttcaccgat aacggtaaaa
    33001 atcatatcgg actcacgaac aggcacatag atattgctta cgttgaatcc tttaccaaac
    33061 aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat tcctgacgta
    33121 tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt gaatccaact
    33181 ttataaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc tgctccaacg
    33241 actactgcca agattactac aggtgcgatg atgcgccaag agattccaga catcaggaaa
    33301 atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg ggccatgatc
    33361 aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa gtccgatttc
    33421 atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat catcaagatg
    33481 tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg aaaccagttt
    33541 ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag tcccattacc
    33601 actaacccca aaccataaag gtaaggagtc agtttccaga tccatttgga attcaaatgc
    33661 atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat ccctacgcct
    33721 ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga gagcagacca
    33781 atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt atcgttcgaa
    33841 gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg ctgattatta
    33901 tagcggtcca gttagataat ggaaagtctt tccatagaca tttgggaaaa attaactttc
    33961 cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc tttagtgtat
    34021 catagaactt tttttgatac atgcaattat ctaccagctg agattctcct cgctgtgaga
    34081 agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca gctgatcaga
    34141 aaccagattg gttcgttgga tgaaataaaa atatcgatgg aaattcaacg gagtcattgg
    34201 cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt ccttcagcaa
    34261 cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat tcatctcttc
    34321 aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa gccacaattt
    34381 gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc tttgtacgcc
    34441 ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg ctacttgttc
    34501 taatgattca tgagaattcg tgatttcgat ctcaaaatgc actttaggaa accttttgat
    34561 cagatgtttc atcaactgag gcaaaagata gacagcaaag gtgtgggaag caacgatccg
    34621 acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt cccattcttc
    34681 taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa gcatctcttt
    34741 tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt tgatttgatt
    34801 cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc
    34861 atatactgct cgaaaagtcg ttaataactt aaacatggca ccacttccat aactttttat
    34921 gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat aatagaaatg
    34981 tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca gctgtctgct
    35041 tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt ggtactaaaa
    35101 tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt attccgatcc
    35161 cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa gactatcacc
    35221 atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata ttcatttttg
    35281 ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca atcatcgcct
    35341 ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt gtttcatatg
    35401 aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc caagtgatcc
    35461 ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt gaagcaattt
    35521 atccaagctg gtttatcatt tacgttggat ttggggtcat cactgtaact gccgggaact
    35581 tcttcccact tattggaaaa attttctttt ggatttcatt gatctgctat gcgttcttat
    35641 tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg acacgtccgc
    35701 tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt aaaaactttg
    35761 ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg
    35821 ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc tatgcagcct
    35881 ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat tacttcaaca
    35941 gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa
    36001 gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa gaacatagca
    36061 aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa
    36121 agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca tgtttttgta
    36181 ctggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
    36241 aagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt
    36301 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttat tcgaaacaag
    36361 aggtcgtaca agacttttgt cacgacctct tgttcttttg tcaaaatact ggtttagtgc
    36421 tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca aatgaaatca
    36481 ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt acccggttta
    36541 ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct gccacagtta
    36601 ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt ttttaaacag
    36661 cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt ctctgttttt
    36721 ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtgtaaa aggaattgtt
    36781 tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg taaaaaactg
    36841 caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg tggttcttct
    36901 gctattgctt caatcgcccc ttccatccat gcgaaagagg aagaaaaagg acagatcatc
    36961 acactagtca atctactggg tactgtgatg atgttgactc tcccttttct tggattacag
    37021 ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggtacact ccaatctgtt
    37081 ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt cgccatgcta
    37141 tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga aaaattcatg
    37201 cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa acgaaaatta
    37261 ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag cttgttttct
    37321 ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga gatcactgct
    37381 ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg tacacgcttc
    37441 ttatcttatg gattagctgt cgggcttctc caaacagtga tggctttact atttatttgg
    37501 ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg gaacaatgtc
    37561 cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat tggcgtttga
    37621 tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt tgcccgatca
    37681 ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc ccaatcgctc
    37741 gcacagcttt cggactatca acagcaacgg ctacttcttt atacgttcgg atttctccat
    37801 aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct ctctttatag
    37861 ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac tgttcttgtg
    37921 tgcgcgttaa aattcgctga ttcgctgatt cgctttctga aattggctga aaagatacag
    37981 cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg tgtattttca
    38041 ttttgtttgc tcctagtcaa tagactggaa cttcctcctt tttctctatc caaaactact
    38101 tgccaactaa taaaaattgt tcttaaaagt ttgataaacc gaggaaacat ggtaaactaa
    38161 atacgttagt ttacaaaaag aatacctgtt cttttacgta aaatcaaatt ttaaaggtgt
    38221 tatttaaggt ttattaaatt ttgaatgtaa accatatata aacatacgat tgaaaggata
    38281 gaaacgatga aagtatcaat tgttgtccca tgttttaatg aagaaaaaac tgttcctctt
    38341 ttttttgatg aggttgaaaa attcaaaggt gcccatgagt tcgaatattt gtttattgac
    38401 gacggatcaa aagacggtac gttagcagca atcaagaagc tagcctcagc ttatccttat
    38461 gtccgctttg tctcattttc cagaaatttt gggaaagaag cggctctgta cgctggattg
    38521 caagcttcta caggagattt agtgaccgtg atggatgttg atctgcaaga tccgcccgaa
    38581 ctgcttcctg agatgatcaa ccggatcact acttctgata tcgattgtgt cggcacccgg
    38641 cgttcgactc gtgaaggcga gcctgcgata cgcagtttct ttgcgaaaaa attctaccag
    38701 ttgatcaata aaatcagcga tacagaaatg gtcgatggcg ctcgtgattt ccgcttgatg
    38761 actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat acaaccgatt ttcaaaagga
    38821 ttatttagct gggtcggatt taaaacagaa tatatttctt atgaaaaccg cgaacgtgtg
    38881 gctggcgaaa catcttggtc cttctggaaa ttattcaact actcgattga tggtatcgtg
    38941 aacttctcgg atgcaccgtt aacgattgct tcatttatcg gcgctttgtc ttgtgtcggt
    39001 tctggtattg caattttgtt cattattttg cgggcattgt tgtttggtga tcctacatca
    39061 ggatggcctt ccatggtctc gatcttcctg ttcattggtg gaatccaatt gttatgtctt
    39121 ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa cgaaacagcg tcctgtttat
    39181 atcataagag aaacagaaaa agtgaagcca aatgaattga agcctcacca ataatatacg
    39241 tcaaatgaag taaacatttt tttaaatgaa aaaggactgt gacaaaagtc ttgtcacagt
    39301 ccttttttcc gaataaatgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    39361 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
    39421 tatcacaacc tctttcatat cttattactt cttcattgct gcttttagtg ctgctgctaa
    39481 tgggctttct tctggttctt ctgcttgtgc atattttttc agcaagcgtc gttcttcatg
    39541 tttggtcatt ttcttcttct gctctttttt atcaagcaat ttttctgtcg ttccatcgta
    39601 tttacaacgg aaataagcac cgtttttacc ttcgatgatt tccattttcc ggtgacattg
    39661 cgggcatcga tgattagaga cttttggatc ttttcttctg cggtagttgc aatctgaatt
    39721 ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg cgcagattgg ccccacattc
    39781 tgggcatttc ttttgggtaa tcgagaaatc ttgatattct tgtttgcttg atttgatctc
    39841 attcaccaag cgttttgttt ctttttcgat tccattcaag aaggttcctg cttgtttctt
    39901 tcctgcagca atagcttcaa ggtctttttc ccagctttct gtcaaatctg gtgttactaa
    39961 agagggattt accaatacta ataattgctt acctttagga gatacttgca gtccttgagg
    40021 ggtacgttcc atcagttcag atttgattag tttctcgata atctctgcac gcgtagctgg
    40081 ggttcccaga ctgtattttt ccatttgacc aagcaagctt gcttctgaca atggtttagg
    40141 cggtgcagat agttcttttt tgatggcgaa atcgggattg attctcattc ctttttccca
    40201 tttgactgtt tccgtctcag tttcagtttc gcctttccat cctgcttgga cgacacgatt
    40261 ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta acttttgttt ggctgacttt
    40321 atggggttga gcaaacagtc ctaaaaaacg agagacaatc atttgataga tttttcgctc
    40381 ttcattactt aatttttcat aacgtgctct gttttcagtt ggcaaaagtg catggtgatc
    40441 ggtcacttta ctgtcttgga atacttttgt ctgttggacg atggctccgt ttttcaaata
    40501 ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat cgttctttca tcgtactttt
    40561 catgtcattt gtcagatatt tgctgtctgt acgcggataa gtaacgatct tatgggtctc
    40621 atacaaactt tgtacaagac ttaatgtttt ctttgcagaa tagccatagc gttgattggc
    40681 ttctctttgg atctctgtta aatcataagg caatggcgca gattccgttt ttaatttttc
    40741 ttggacttct gtgacagttc ctttgcttgt actgatcaat tgaacaagtt tttgtgcttc
    40801 ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt ttggcttgtt cttttccagc
    40861 ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc tcgattttct tttcttggtc
    40921 tctcactaat gctagtgtcg gtgtctgcac acggcctgct gataaattat ccgcgtactt
    40981 cacagttaag gctctggtca cattcaatcc taccagccag tccgcttttg cccgggcaag
    41041 agcagattca tacaatgcat catattcttt cgcaggtctt agttgcttga atccagtttt
    41101 gattgcttta tctgtttgag aagagatcca taaccgcttc actggtttat tgaaatgcac
    41161 atactctaag atccagcgag caacaagttc cccttctctt ccggcatctg tcgcaatgac
    41221 tgcttctgag acatcttttc gctgagccag ctgcttgatt gcttttaatt gatgtcctgt
    41281 tttaggaagt ggtttgattc ctagtttttt agggatcatt ggcaacgtgt ccatttgcca
    41341 gctctgccat tccttattca gatcttccgg cattttcaat cctaataaat gaccaagggc
    41401 ccaagtaacg atcaccttgg gtccttcata ataatttttg tgcttttgat tagcaccaag
    41461 caccttgctt aggtctttcg caacgcttgg tttttccgct agaattagtt gtttcataaa
    41521 attcctttct gctttctact gtgatgggct gatgattttt caacaacgca agtcccttgt
    41581 attgctgtaa atcatcgtca cactcttcac accagcgctc atctccgtca ttccaaaagg
    41641 cgctgagaaa gttacttttt gcattgatat attcataatt ttgatggatt tcgttccatt
    41701 gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa tgtttcttct tcttcaatat
    41761 cttcttccca gtcttcaaaa aaccaccaag gctcattgtc cccgaacatg gtaattactt
    41821 gatacatact cgtgctcctt ctttcagctt ttctagactc atcttagtca cttttcaaga
    41881 aagttgcaag aattttctct tataaaaggg tcatttaatg aacatcttaa aattgttaca
    41941 aatgaatctg attgtttaag gcaaagtagc gataaagtta ttgatcatcc aaaaatagct
    42001 gtcttctgga tcgattgttg gttcttttgt aaagtatcct aaaaaacgta aggaaagata
    42061 accgtggatc atactacgaa gttccctgct tcgatgaact ttttcttctt tcgttaattg
    42121 aaaagcctca aatatctgca aaatgatctg attcgtttca tggattccat ctaataaaac
    42181 agcttcattg gtatgcggta cactgatcag taactcatac actgcttgat ttgaaaaagc
    42241 aaaggatcga taagtttctg catagatctg cagtgcttcc tgaccacttt ttcccacaag
    42301 agcccgtcgc agctggtcat taagttcctg cagaagtttc gctgttagcg ctgttttgac
    42361 atcacgaata tttttgaaat ggttatacag cgagggagcc ttgatatcca agcgtttggc
    42421 taaatgctga aaggttattt gctgaacatc catctcttct gctagttctc gaaagcagtc
    42481 gatgattttc tcttgagaca gatttctttt tttcatttcc atgtttcctc tcataaaata
    42541 aatccttcac tctttcagtt taccacgaaa aagaaagaga aaaaagagac tttcattgat
    42601 aaactacata ttgtagttta taataaagag ctgtagatga aaattttcag aaagaaggag
    42661 tctaattggc tgcaagaata aaggaaaaat tatttaggaa aagagaaact tgggaacaaa
    42721 acttgtttgt cttatggttt ggaacattta tggctggtat cggatttagt ttagtcatgc
    42781 cttttatgtc actttacata gatacgttag gtaattatag tacgtcacaa ttgaactttt
    42841 ggagcggatt gactttttca tctactttct tggtgactac gcttatctct ccttggtggg
    42901 gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt gcgtgcttca ttaggaatgg
    42961 caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta tcaattagtc gcattgcgtc
    43021 tgcttcaagg gattttttct ggttatatca gtaatgccac tgcccttgtc gcaactggga
    43081 cgccaaaaga aaaaagtggt caagttcttg gaaccttagc aactggttct gttacaggaa
    43141 cattacttgg accgcttctt ggcgggatct ctgcttcagc gtttggctat cgccctacct
    43201 ttttcattac agggatcatc ttatttttcg tcttcttgtt gagtctgttt ttcgtccatg
    43261 aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc caaacagatt ttcaaagact
    43321 tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac gatgatcatc caagcatcca
    43381 acaattccat tagtccaatc atcagtctgt acattcgcca attgctgcat ggacatggga
    43441 atgttacgtt gatcagcgga atcattgctt cgatacctgg gattgccact ttgatagccg
    43501 ctccaagatt tggtcgctta ggtgacaaga ttgggagtga aagaatactt gcgattggtt
    43561 tgggttttgc gattctggtt tatatcccaa tggcattcgt cacgaatgtt tggcaattag
    43621 ctgccttacg ttttttgatt ggtatctctg atgcttgtct gctccctgct gtccaagcat
    43681 tgatcacaaa atattcgcct catcaagctg ctggacggat cttcagttat aaccaatctt
    43741 tccaagcaac tggtaacgta gtagggccta tgatcggctc cagtgtttcc agtgcatttg
    43801 gttatcgcgg cgtctttctt tcgacttctc ttcttgtttt ggtcaacttc ttgcttgtac
    43861 gacacaatac acaagagatc catcagcaag aaactgcaga agaaaaagct tcaggagtct
    43921 ccatagcttc agggcactct ccttctcata gacaataagc gtaaatgaaa aacactgctt
    43981 gaacagttgt cacagactgt ccttgcagtg tttttgttta ggcctgtttt ttcaagaact
    44041 cagataagag gaataatccc ttttccaagg tttctttttt acaacagtaa cctagtcggg
    44101 catgcttcgg taaatcaaag gcacttcctg gtactaatag tactcctgtt tcttctaata
    44161 aattgataca aaatgtctgg tcatcttctt cgatggctaa tttaatgaaa gaagtagaga
    44221 cgtaattggg agcgacaagt tcaactttcg gttcattggc tacccattgt tttaaaatca
    44281 caaggttctc tgtaatgatc tttttgtttc gcgccaatat tttttcttta ttcttcaatg
    44341 catgtacagc aagatcatct gacaggactc cgccgcaaat catcgtgtag tctcgatact
    44401 ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca gccaatacga atacctggaa
    44461 tggagtaggt tttggatagg gaattggttg ctataccttt ttcatagaca tctacgatcg
    44521 acaagaattc acctttatcc gttaaaggcg catatacttc atctatcaat acatatgcat
    44581 ctactgtacg agcaatttct gcgatttttt ctaaagtttt ccgatctagc aatgtgcctg
    44641 taggattgtt tgcactattc aaacagatga tttttgtttc aggtttgact aaggcttcta
    44701 gctgttctaa atcaaactgc caatcttctt cttccttcaa atgaacaaag tcaacagcgg
    44761 cacctaaaga tttggggatg tcatataatt gctgataagt aggaagcatc gaaacaacat
    44821 ggtctcctgc ttccactaac gcgtatagag ctaaaagatt agcaccagtc gctccattcg
    44881 tttgtaagat gttttccgga tcgacggttt gataaagaga agcgacttct tgtttgaaaa
    44941 catctgatcc ttcgatccag ccatagtcca atggttcgtt tttgcgtttt tcgaaaaatt
    45001 tacttacgct agtcccatct aagccaacga gttcgtctaa tgtcaatgct tcgatcgagc
    45061 tttgtgcaat atcatatacc gctttctttt cccatttatt tagccattct tctacaccga
    45121 aatttgcgat tttcatgttt ctttccccct tatatcttga tccagcctac agcaagcaac
    45181 ccgattgaaa gcagtgcggc cacacaaatc agcccaatca ttactttttc ttttcccgta
    45241 atttgatgtt tggtttcacg acaagcgatc cagtagaagc aaaaaccagg aagataggca
    45301 atactacata aaaggacata agaaaatcct gctaaaatga tgcccatcaa ttcaaaaact
    45361 acagcaatcc caccgatcac tacttgtttt ttgattcctt cttttaaaga aaactggact
    45421 tgatagatcc cgacaagtag ataacaaatc aaaattgccg ctgtacataa agaataggca
    45481 aattgatagg cataatcagt aactaagaaa gtcaataaga atagattagt caagattgca
    45541 gtaacaacta acgaataagt tggggcattt tttttattcg tttttcccca acctttcggc
    45601 aacagaccgt ctcgagccat caacatcgtt gtttctgctg ggagcatcgt ccaagacaac
    45661 caagaaccaa tgatcgaaat aatcaagccg atattgatca gcgctgctcc ccaatttccc
    45721 acgattcctt ttaaaatatt tgccatagca ggttgtgaaa tagttgctaa ctcttcttga
    45781 gataagacgc cgtatggcaa gatcgaagca aggatataga taaacaataa acaaagcaga
    45841 ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg cgcggttggc taaaacactt
    45901 gcgccttcga tccctacaaa gacccacatc ataaccatca aacaaccttt aatctgcaac
    45961 cagatcgttc cttgctgccc tgttccattc actagattat cagatacgtt accccaaaaa
    46021 tcagcagtga atatattcac tttaaatgcg ataaatacaa atattaaaaa taggaataat
    46081 ggcactaatt tacagatcgt cacgattgta ttgatgacgc tcgctccttc aactccattg
    46141 tttactaata aagtcagacc ccaaataaaa atacttgcaa ataaaataga aggaacattt
    46201 tgtccacctt tgaatatcgg gaaaaattct cccatggcgc tcatcagcat cgttgcaaaa
    46261 gcaacatttc ccagccacgc agacaaccag taaccccaac cactgatgaa gccacctaat
    46321 ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat ctagatctgg acgcttttct
    46381 cctaaatgat tcagcgataa taccaaagct aaaataccaa tcccaacgat tatccatgaa
    46441 aggatcgctg gccctggagc tgcagaacta gccagatcac ttgtgatccc aaacacaccg
    46501 cttccgattg acgaactgac aaccagagca accagtcccc atttgctaat acccgtcgat
    46561 ttcttttcca tttccatcac cctcacttat aaaattgaac aaatcacttg caaaagattc
    46621 ccttctattt tgcaactgct cttgcttaac aaaaagcaac tttattcata ttattggata
    46681 ttatgaactt tttacacaag tatttcaagt ataaaatgaa taaaaattaa ttttattgca
    46741 aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt tgttagccat agctgttttg
    46801 attcatttaa tatcgcttaa acgtattgta aaacttttgt ttcttgatat gcgtggtcga
    46861 tcaaaccacc acccagacat tccattccat cataaaatac aactgcttgt ccaggagtga
    46921 ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt atcatccaat aagcgaactg
    46981 tcacaggaac gtcttgctga cgataacgga atttcgctgt acacttgaat tcttttggca
    47041 ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa gcttgtggca tacaatgcag
    47101 gatgatggaa cccttgcccc acatataatg tgtttgtcgc tagatctttt cctacaacaa
    47161 accagggttc ttgcgaatct cctccgccac cgatccctag tccttgacgt tgaccaatcg
    47221 tataatacat caagccagca tgttgtcctt tgacttcacc atcaagagtg accatatttc
    47281 ctttttttgc tggcagatag ttgctcaaaa attgtttgaa gttcttttca ccaataaagc
    47341 agattcccgt tgaatctttt ttcttagcag tcgcaaggcc tgcgcgttca gcaattgccc
    47401 gtacttctga tttttccatg ccgcctagag ggaacatcgt ttttgaaagc tgttcttgtg
    47461 ataattggct aaggaagtaa gtttgatctt tattattgtc tactccacga agcatgtgga
    47521 ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc agtagctaca taatcggcgc
    47581 caagatccat tgcataatcc aagaatgctt tgaatttgat ttctttgttg cacatcacgt
    47641 ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa atattcaaat acacgatccc
    47701 aatattcttt ttcaaaattc acagagtaat aaggaatccc gatttgagca gctactttcg
    47761 ctacatcctt ataatcttcc gtggccgtgc atacaccgtt ttcgtccgtg tcatcccaat
    47821 ttttcatgaa tattccgatc acgtcgtatc cttgttcctt taacaaaagt gctgttacag
    47881 atgagtcgac accgccgctc atccctacga caacgcgtgt tttgctgttg tctgtcatga
    47941 tatcaccatc atttcaatta gattctgaaa catctacgag ttgaaggtcg caacttttca
    48001 gtattttaca ttatacgtca tatttctgaa aatttcatcc cttaattatc aagtcgtaaa
    48061 aatactgttc tactggacct attcagtaca caaacatgat agtcaaccaa aaaatcactc
    48121 atcttttgag cattcgatat aaaaaattgg catactcata aaaagggcca gcctttcggc
    48181 aataaggaat aattttataa attgtgaaaa actatttttc aaaattgctt cttatcgctt
    48241 aaagctgcac gtctcattta caaccttttc tttttagatg ttatgagttg ctcagataag
    48301 tgattttgtt tactctaaga aaaaagggag gcaaaagata catgaattac tttatcagtg
    48361 atatgcactt tttccatgaa cgcttattag gaaaaaacga ctttgctccg cggctctttg
    48421 caaatgtgga tgaaatgaac caacagttga tcactagctg gaatgcagta ataaaagaaa
    48481 ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc gcaatatgaa aaagcaaatg
    48541 cagaaatttt ggaactggtc actcaactgc aaggaacaat ccacttgatc aaagggaacc
    48601 atgacagtcg cgcttttttc cgttatttag aaaaaaatga tcctaggctt actgacggaa
    48661 gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa attcgaccat caacaatatt
    48721 acttgactca ttatccgatg ttgctcgggc ctaacgacac tatccgcaat cttcatggac
    48781 atatccatca ttacagcgta cctatcgaga acgatgtcaa tgtaggcgta gacgcaccgg
    48841 aaagagaact cttaaaggaa aaactaccat ttggcactcc gttatctgag caacaaatca
    48901 aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg aaaaaactgc cctatatcaa
    48961 gtattaaaca aataaagata attactcata tttttcattt gagtaggctt attgccacag
    49021 gaacattagg atcagaaatg gagttgtaac aagacttctg tcacaactcc attccatact
    49081 attcgtcttt ttgctcatgg tgttctttta ccagtttttt ctgtaattct ttatcataat
    49141 ttcgggcagc tgacgttaaa aataaaacga ttccgcataa taaaatcccg attccggaaa
    49201 acaagaacat tttttctacc cctaaacgat cagctaatgg tcctgcaaat aataatccaa
    49261 ttggacctgc caggttcatc aaagagttga agttccctaa gacacgacct aaaacattcg
    49321 attcataact ctgctggatc attgccatca gcagtgtatt gaaatatggc gttgcacaac
    49381 ctgctcctgc attcaagata agaaagtaca agaacccttg actatcgttt ggaaccaatc
    49441 cgcttgcacc gatcgtcagg cccataatga tgtaggctat aataataggt ttcattcggt
    49501 ctttccatgt tcctataaaa ctgatcagtg caccgcctaa gagcattccg actgcgtaga
    49561 ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa gtaatctagc gtcatcaatg
    49621 gatacagact gattgctggc atgaacaaca gcatcacaaa tgcaccattg atcgttatgt
    49681 accataaccc tttattatcc atcaattgct gcagcccaaa ttttgcatct ttaaaaatag
    49741 taagcggaac ttccaatatt tcaggagaaa cttttgggat cttcacaaaa atcaataagc
    49801 cgacaccaaa aactgccccc aatacatcta ataaaataag gtaattgaca ggaacgactg
    49861 aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt cgctgattgg accattccta
    49921 actgtccatt cgtccttgtt atgtgagaag atggaacgat tgtcggcata atcgactgga
    49981 tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa caaagacaca aaaactagcc
    50041 aaatcgggaa ggattctgaa atcgttccga ctactgctaa aataagcgcg aaaatcgcta
    50101 caataatatc cgtgacaatc aaaagagctt ttttatccca gcgatccact agcggtccta
    50161 caaatgggct tagtaaaacc atgggaatca ttcctagtaa agtagcaaaa ctcaaaattg
    50221 tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc atattggacg accatactag
    50281 tgattcctga caaaaactgt cccgataaaa agaacaaata atttctcttc caattcgtta
    50341 aaacgaccga ctgttcattt tccataaaca tccctctttt ctttttcatt ttgtatagcg
    50401 tttgcacaac aatcattata cgggattctt ttttcaaaaa ataccttttt ccctaaataa
    50461 caatcgtttc aatttaaatt tctttactta gaaacaataa atcagcaaaa tattaaaaat
    50521 aacaacatgt gttaaattta aaaataaaaa agacaactaa tttgcgtcgt ccttacaa
//
LOCUS       DAFEZB010000012        50012 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFEZB010000012
VERSION     DAFEZB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.012"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(13..1023)
                     /locus_tag="LP859_000753"
     CDS             complement(13..1023)
                     /locus_tag="LP859_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3028728.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1203..1547)
                     /locus_tag="LP859_000754"
     CDS             complement(1203..1547)
                     /locus_tag="LP859_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL3028729.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1540..1974)
                     /locus_tag="LP859_000755"
     CDS             complement(1540..1974)
                     /locus_tag="LP859_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL3028730.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2112..2849
                     /locus_tag="LP859_000756"
     CDS             2112..2849
                     /locus_tag="LP859_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028731.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2842..4053
                     /locus_tag="LP859_000757"
     CDS             2842..4053
                     /locus_tag="LP859_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028732.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4257..5039
                     /locus_tag="LP859_000758"
     CDS             4257..5039
                     /locus_tag="LP859_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL3028733.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5109..5771
                     /gene="trmB"
                     /locus_tag="LP859_000759"
     CDS             5109..5771
                     /gene="trmB"
                     /locus_tag="LP859_000759"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL3028734.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5869..7623)
                     /gene="ade"
                     /locus_tag="LP859_000760"
     CDS             complement(5869..7623)
                     /gene="ade"
                     /locus_tag="LP859_000760"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL3028735.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7755..8684
                     /gene="tadA"
                     /locus_tag="LP859_000761"
     CDS             7755..8684
                     /gene="tadA"
                     /locus_tag="LP859_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL3028736.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8641..9690
                     /locus_tag="LP859_000762"
     CDS             8641..9690
                     /locus_tag="LP859_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL3028737.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9687..9965
                     /locus_tag="LP859_000763"
     CDS             9687..9965
                     /locus_tag="LP859_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL3028738.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9940..10410
                     /locus_tag="LP859_000764"
     CDS             9940..10410
                     /locus_tag="LP859_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL3028739.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10427..10729
                     /locus_tag="LP859_000765"
     CDS             10427..10729
                     /locus_tag="LP859_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028740.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10746..11117
                     /locus_tag="LP859_000766"
     CDS             10746..11117
                     /locus_tag="LP859_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL3028741.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11089..11448
                     /locus_tag="LP859_000767"
     CDS             11089..11448
                     /locus_tag="LP859_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028742.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11620..12627
                     /locus_tag="LP859_000768"
     CDS             11620..12627
                     /locus_tag="LP859_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3028743.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12652..13836
                     /locus_tag="LP859_000769"
     CDS             12652..13836
                     /locus_tag="LP859_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL3028744.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14273..15856)
                     /locus_tag="LP859_000770"
     CDS             complement(14273..15856)
                     /locus_tag="LP859_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3028745.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16434..16577)
                     /locus_tag="LP859_000771"
     CDS             complement(16434..16577)
                     /locus_tag="LP859_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL3028746.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16800..17279)
                     /locus_tag="LP859_000772"
     CDS             complement(16800..17279)
                     /locus_tag="LP859_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3028747.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17352..17636)
                     /locus_tag="LP859_000773"
     CDS             complement(17352..17636)
                     /locus_tag="LP859_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028748.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17714..17908)
                     /gene="ssrS"
                     /locus_tag="LP859_000774"
     ncRNA           complement(17714..17908)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LP859_000774"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17980..19266)
                     /locus_tag="LP859_000775"
     CDS             complement(17980..19266)
                     /locus_tag="LP859_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL3028749.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19404..20075
                     /locus_tag="LP859_000776"
     CDS             19404..20075
                     /locus_tag="LP859_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL3028750.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20041..20529
                     /locus_tag="LP859_000777"
     CDS             20041..20529
                     /locus_tag="LP859_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL3028751.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20542..21489
                     /gene="prmA"
                     /locus_tag="LP859_000778"
     CDS             20542..21489
                     /gene="prmA"
                     /locus_tag="LP859_000778"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL3028752.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21490..22254
                     /locus_tag="LP859_000779"
     CDS             21490..22254
                     /locus_tag="LP859_000779"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL3028753.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22487..24700
                     /locus_tag="LP859_000780"
     CDS             22487..24700
                     /locus_tag="LP859_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL3028754.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24711..25157
                     /locus_tag="LP859_000781"
     CDS             24711..25157
                     /locus_tag="LP859_000781"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL3028755.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25328..26587
                     /locus_tag="LP859_000782"
     CDS             25328..26587
                     /locus_tag="LP859_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL3028756.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26676..27623)
                     /locus_tag="LP859_000783"
     CDS             complement(26676..27623)
                     /locus_tag="LP859_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3028757.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28017..29309
                     /locus_tag="LP859_000784"
     CDS             28017..29309
                     /locus_tag="LP859_000784"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL3028758.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29324..31093
                     /gene="aspS"
                     /locus_tag="LP859_000785"
     CDS             29324..31093
                     /gene="aspS"
                     /locus_tag="LP859_000785"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL3028759.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31202..32077
                     /locus_tag="LP859_000786"
     CDS             31202..32077
                     /locus_tag="LP859_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3028760.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32343..34226
                     /locus_tag="LP859_000787"
     CDS             32343..34226
                     /locus_tag="LP859_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL3028761.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34301..34585)
                     /locus_tag="LP859_000788"
     CDS             complement(34301..34585)
                     /locus_tag="LP859_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL3028762.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34575..35309)
                     /locus_tag="LP859_000789"
     CDS             complement(34575..35309)
                     /locus_tag="LP859_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL3028763.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35438..36061
                     /locus_tag="LP859_000790"
     CDS             35438..36061
                     /locus_tag="LP859_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL3028764.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36253..37374
                     /locus_tag="LP859_000791"
     CDS             36253..37374
                     /locus_tag="LP859_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL3028765.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37381..40509
                     /locus_tag="LP859_000792"
     CDS             37381..40509
                     /locus_tag="LP859_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL3028766.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40528..43722
                     /locus_tag="LP859_000793"
     CDS             40528..43722
                     /locus_tag="LP859_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL3028767.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43868..44575)
                     /locus_tag="LP859_000794"
     CDS             complement(43868..44575)
                     /locus_tag="LP859_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL3028768.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45340..45740
                     /gene="spxA"
                     /locus_tag="LP859_000795"
                     /pseudo
     CDS             45340..45740
                     /gene="spxA"
                     /locus_tag="LP859_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
     gene            46072..46725
                     /locus_tag="LP859_000796"
     CDS             46072..46725
                     /locus_tag="LP859_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL3028769.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46951..47949
                     /locus_tag="LP859_000797"
     CDS             46951..47949
                     /locus_tag="LP859_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL3028770.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48125..49933
                     /gene="pepF"
                     /locus_tag="LP859_000798"
     CDS             48125..49933
                     /gene="pepF"
                     /locus_tag="LP859_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL3028771.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16175 a         8742 c        10458 g        14637 t
ORIGIN      
        1 ttttgttttc ttttattttg atgaatcagt tgtttcttca gttgaagatt cttttgttga
       61 atcagttgat tttgtgtcag aactctttgt tgaagctgta gtttcacttg agtctttcgt
      121 acttgatgaa gttgttgtga atgcactcaa cacattttca aaggcatcat ctttgatttt
      181 tacatttgca tcttttaatt cttcaccgat tactttagtt gtgaatgcat tgtcacttag
      241 tttagtttca gtagcgatat cttttagttg atctttgtat ttatccatgt cattgccttt
      301 attttggttt ttcaccattt tgacaacgta gtattctgtc gcataagaag taggatttgt
      361 tgtagtaata acatcggaga tctcaccgtc tttcaatttg aaagctgctt cttttacttc
      421 tgcaggaata gttgttgtcg ttgagtcgaa tttaacttta ccgccatctt cttttgtctc
      481 tgtatcagtt gatttgtctt tggctaattt agagaaatca tctccatcat ccgctgattt
      541 tttcacatct tttgcttcat cttcacttga taatttgatg atttgtgctt ctacttctgg
      601 atggaaagat ttccaagcag ttttcaaatc gtcatctgta atatctacgt gagctttcaa
      661 gccagcttca aaagctaaat tgttacgaat atattcttta taagtatctt tcgtatatcc
      721 agcagcttct aattgtgatt caaaagtatc acctaatgat ttcgcttgtt tgtcgtattc
      781 tgcatccact tgcttatcgg ttactttatc gccatatttg ttattgaata ctttataaat
      841 gatcatccgt tgtactaagg attggttcga tgattcaagt tttgcttcat catagaaatc
      901 agatacagtg atcgttccgc ctttcatcgt tgcgatatct ttattagtat cgcctgaaca
      961 agctgctaga gttaaaacag ccaacgcact agtagctgcc aaaataattg attttttttt
     1021 cattaaaatt tgcactcctg taattagtta ttatttttat atattaacta taccatatcc
     1081 catttccttt aaaaacgttg tcgtggtagt ttcaaacaaa attcaaaatt tcatcacatt
     1141 cctaggaaat acgcaaactt ttctcactta tatagttaaa tctgttgaga aaataatcat
     1201 tattatattt gttcaggata ctctggcaga tcagctttta gtttttctac ttgttcattg
     1261 atttgtgtga tacgtggttc ggcttggaat ttaaaatctt cgatatcttt tttgattgct
     1321 tcttggaaag caggaatcac agattcagct gtttcttttg tttcgatcaa tgcgctttta
     1381 aaatgattca ggctgttatt taaatcattt gtcagagttg ttgcttcttc caattcttcg
     1441 atcagtttct ttcttgtttc attcccgctc ctgggcgcca ataacagacc tccgacagtg
     1501 ccagcagcag ctccaaataa cagtcctttg aaaaagtttt taatcatcgc aggacacctg
     1561 ctttacgatt gtttcttgaa tagcttttag ttcttccgat ccataactat cttgatgatt
     1621 gacaaagtga atagaaaaat catcctcttt gctgtagcga gggactaaat ggatatgtga
     1681 atgaaaaaca gattgatagg ctagttcacg gttgttattc aagatattaa gacctttcat
     1741 ttctggaaaa gctttttcca atgcttgagc aatcttaggc acacgcgaaa aaacctctcc
     1801 tgcaagttcg ggatcatatt caaaaatatc tgctacatga cgtttaggca caactaatgt
     1861 gtgtcctttc gttgcttgag agatgtctaa aaaagcatag accttgtcgt cttcatatat
     1921 tttatagctt gggatttctt gattgatgat tttgcaaaaa atacagtttt ccatataaaa
     1981 catcctttct ataaagaata aatacttcag cttaacttta ccataagtta aagctattgt
     2041 aaggaaaaaa acaaaatacc gtcattttct tcgtggtata ataaataaaa ataaatggcg
     2101 gaggaaaaag aatgagttta attatcgatc atgtcacagg ggggtacgga catctccctg
     2161 tgttaaaaga tatcagtttc gatgtacagc ctggagagat agtagggttg attggactga
     2221 acggagcagg gaaaagtacg accatcaagc acattatcgg tctgctggaa gcttcaaaag
     2281 gaacgataga aatcaatcat ctatccttaa agaaaaatac ggaagaatat cgcaaacaaa
     2341 ttgggtatat tccagagact ccttctttat atgaagaatt gacattgaga gaacatatcg
     2401 aagtcactgc gatggcctac gatataccta tggatatagc aatgaaaaga gcagaaaagt
     2461 tgttggagac ttttcggtta tctaatcgtc ttgactggtt cccagctaat ttttcaaagg
     2521 gaatgaaaca gaaagtgatg atcttatgcg cctttttgat cgagccttct ttatatatca
     2581 tcgacgaacc atttttaggc ttggatccac tagctatcaa tgcattgctt gaattgatgg
     2641 tagaaatgaa aaaagaagga gccggtattt tgatgtctac gcatatttta gctactgcag
     2701 aaaaatattg tgatcgtttt gtggtcttac ataacggaga gatacgagca gacggttctc
     2761 ttgagatgtt gagaaaagaa tttcagctgc cggattcttc tttggacgaa atctatattg
     2821 ctttaacaaa ggagcaggca catgagtagt ttttttcaaa aacgtttagc aagacatcaa
     2881 aagaaaatga tgaaatatat gcgttatgta ttgaacgatc attttgtttt agtttgtctt
     2941 ttcttgattg gcggtgtggg attatattat tctaattggt tgaaaaccct gcagccaccg
     3001 tttgcaattg gagggctcat aatctctgta ttttggatgc tctgtttatt cgtgggtaag
     3061 atggctactt ttgcagaggc agcagatatt gtttttttac ttcctaaaga aaaagaaatg
     3121 caaacctatt tggaacgagc ttttcgctat tcttgcattt ttccgttgat ctttttgata
     3181 ttggcatgcg gattggccat gccattagtg gtggttagta cggagcaatc cttttcgatg
     3241 tttttcgtct atctgatcat attatggagt ttgaaattca gtcatctgca aatcaagaga
     3301 gcagaattgt ttcgactaga tcaacaagtt attcgttatt ggaaattagg ctggtttttt
     3361 tcttctctga tctttttgtt tctatcattt tattggctaa tatgggcagg aatagcaggt
     3421 gcattagtac aggcaagtct atattactgg tttttgtgga agcaacaaga aaaaggattg
     3481 gattgggaga aaatggtgca tgtagaagaa cataggttgc atcagatcta tcaatttatc
     3541 aatttattca cagatgttcc tgaaattacg gcaaagatca aaagaagacg ctatttggat
     3601 cctatattga ctcatattaa aagagaatca aaaaacactt atctttactt gtttgcaaga
     3661 agattggcaa gaggttctga ttttagtggt ctttatttcc gattagtaat tataggtggg
     3721 ttattgattg ccggagtagt cgacttttat tttagtctga ttatcggttt cttattttta
     3781 tatttgatcg gtttccaact gttaccttta gcgaaccagt tccgctatat ggttctcgtt
     3841 cagctttatc caatccataa gaatcagaag aagcaggcga ttcagtatct actcacatgg
     3901 gtattaatta tcgcgtcggt tatttttggt ttgattgctg cagtcgtatt agatgggcag
     3961 cagcgatggg ttccgctagc ggcttatttg attgttacta tgctctttgt taaactatat
     4021 gtaccaactc gtttgaagaa gatgtcagac tgaaaacaaa gctgtgtagg gtaaaaatga
     4081 gaatgaaaaa tagagcgttg acagtaagaa tgctgttcgt tctatttttt ttgagaaaag
     4141 ctggtttttt gaatggcaat taaatgaaaa agaacgtata tgtgcttgac gtgtagtgtt
     4201 caggcgacta gaataataag tactgcaaaa caaagagtaa gaggacggga gaatgcatga
     4261 tggagtttca gttagaccaa gagtggcggt tacgtcccat caaaggcgac acaggaaaaa
     4321 catacatagg catgcgtgat gaggacaaag tctttattaa acgaaacacg actccgatgt
     4381 tggctgctct ttcaagggaa ggaattacac caaaacttgt ttggacaaaa cgaacgggga
     4441 atggcgatac tttgacagca caagagtggc tggaaggtcg cttgctgact cctaatgaga
     4501 ttggtcaaag aaacgacgtg attgatgttt tgtaccattt acatcattca aattctctta
     4561 aaaccatgct gacccgtatc ggtggtcgtg tcatgacgcc ggagaagatg ctgcaaaaat
     4621 atgaaaaaga attaccggaa gaactccaga aaaatcaatt tctgcaaatt gtccatcgtt
     4681 ttcttcgtga gcagatcccg tcaatagata aggaaaactt atcagtagta cacggagatg
     4741 taaaccatcg taattggtta gtatgtaaaa attatttata tctagtcgat tgggattcaa
     4801 tcatgtttgc agaccctgcg gtggatattg caacgatttt aggaaattat gtgccactgt
     4861 ctagctggaa tcagtggctg atcagttatg gcatacgccc gactgacgaa atgcttgaaa
     4921 aatttcattg gtatgcggcc atgaatttgc tccaagaaat caccagatat tgcaaacgtg
     4981 aagatcatcg acggatgaac gaagaaattt tacaattaaa acgaattttc agtggctgag
     5041 gtttggccca aactctgcgg agatttggcc agaccttttt aattagagga aaggaatgga
     5101 gataaataat gcgtgtccga aatcgtccgg gagcagcgga aatgttagcg gctcacccga
     5161 attttgtaat tagtgatcca acattgtgga agggaaaatg gaatgaattg tttgaaaacg
     5221 atcatccaat ccacattgag attggtatgg ggaaaggcca gttcatcact ggaatggcaa
     5281 aagcccatcc tgaaatcaat tatattggag tagaaatgca ggtcagtgtt gtttcgattg
     5341 cactagacaa gttgatcgaa caaccgcttc ctaacctgaa acttctacat gtggatggat
     5401 cagcgttgac agagtatttc gctgacagcg aagtagatca aatctatctg aatttttccg
     5461 atccatggcc aaaaaaacgt catgaaaaac ggcgattgac atataagact tttcttgctg
     5521 tagatgaaca gattcttcgt ccaaatggag aaatccattt caaaacagat aatcaaggat
     5581 tgtttgagta ttctctggca agtttttctc aatatggcat gatcctcaaa caggtatggt
     5641 tagatttaca tcaaagtcaa tttgaaggca atattatgac ggagtatgaa gaaaaatttt
     5701 cttcaaaagg tcaacgtatt tatcgagtag aagctcaatt tcaagataag accaaaaaga
     5761 aaagtctgtg aaactaactt tctgctttca taaaaacatt aatatccata aataaatccc
     5821 agaaaatgaa cattacagat gtaatgtatt ttctgggatt tatttgattt aaaatgtatt
     5881 tatttcatcg gtttttccac aaggtggaat caattggaag cgagaaaatg aaaataatcc
     5941 tttgtcggtg atcttcaatt cgggtatgac agataatgtt aagaaagaaa gtgtcaaaaa
     6001 aggatcaaag gttttagctg ctcctagttg atgtgcttta gttattaatt cattcaactg
     6061 ttcatagacg atcgaaaaag gttcctgtgt catgattccg cctataggaa gagaaacaca
     6121 agctatttcc tgctgtttgt tgacaacggt catcccgcca cctttttgaa tcaaaagatt
     6181 agcagcatac agcatatcct cgtcgtttgt cccaacgaca actagattat gcgagtcatg
     6241 ggcaatcgtt gtcgctatcg ctccttgttt cagctgaaat cctttcacta tgcctaatcc
     6301 aatgtttccc gtttgatggt gacgctcgat caccgcaatc tttaattggt ctttttcgat
     6361 tgaaggaatg aatcgttcgc cttgtcggtc cactttttct accaaatctt tagtcactag
     6421 actattcggg ataatttcga tcacatgggc ttgtgaagaa gaaagaagca gctctaacga
     6481 atgtggattt aatggttgag cgtttagttt tggcagacaa cacgtatatg aatttgaatg
     6541 atcagcaaat aaatggtctt ggagttgccc agattgaaca actaatgttc ccttatagaa
     6601 aacttcatga attggcagct tggtaagatc gtctgttaag aagaaatcag cttgataccc
     6661 aggggcaatc gcacctagtg ttttcaaatc aaaacattct gctgcattca atgttgccat
     6721 ctgaatggct tgtaatggat cgattccaag tgcaatagct tgacggacga tatgatcgat
     6781 tgatccttct tttactaaat cattgatcag tttatcatcg gtgacaaaga agcatcttct
     6841 tgaattttca ggtgtgatcg ccggtagtag cgatgccaga tcttttgcaa cggtcccttc
     6901 tcgaaccatt aaatacatac caagatccaa acgttcttta gcttcttttg cagttgtagc
     6961 ttcatgatcc gtccgaatac ctgctgcagg ataaacattt aactcttcca tgccgatacc
     7021 agctgcatgc ccgtcaattt ttttcttatg ctggtgcatc aattggatct tttctaggat
     7081 gtctgtttca ttatttgcta ctgcttgata gttcatgact tcagctaagc ctaaaacttc
     7141 gggtttttgt aaaaaagggg ctaatttttt tgcatttaaa tttgcaccat tggtctcaaa
     7201 aggggttaca ggtacacaag aaggtagcat aaaaaagata ttcatgagtg tttgtcttgc
     7261 atcttctatc ataaactcaa gacctgtact cccagcaatg tttcctattt catggggatc
     7321 tgtcactaca gtagtaactc catgaagcaa ggatactttt gaaaattcgc ttggtgataa
     7381 aagtgagctt tcgatatgca catggccatc gatgaatcct ggcactagat attgcccatt
     7441 agcattatgt acgctttttc cttcgtattc accgattcct acgatcattc cttcgcagat
     7501 tgctacatct ttttccatga tttctttcgt aaagacgttg acgatcttag catgcttgat
     7561 cactaggtca gcaggctttt ttttacttgc tacttcgata tatttcctta atgttgtttc
     7621 caatggagtg cctcccaaga taaattttta taagtataac ataaattcgg tttttgccaa
     7681 tatttttttg taaattcatt ttatttgaaa caaaaagatc aaaaagacgt atgttttttg
     7741 tggaggggag aataatggaa ataaaacgat tatcggatca aatgataagt gaaagttttg
     7801 ccaaacatgc ccaagacgta tatatattgc cagcttcaga agtattttac atctattttc
     7861 gctatggaaa aaaacgaaca ttctggaaga aagtctcttc ggcagatgga cagcaactga
     7921 tctcaagatt taagtatttg ggacagatgg atatcggaga aaaaagaaaa gctcagttag
     7981 gtgcatttac ttattcttta gaaaaacagg aagttcgtct gcggttgtct tctgtaggag
     8041 attaccttca acgagagagt ttagtgattc ggctattata cggtactttt gaaacacccc
     8101 atcgttgctt tagttctcga gattttgcaa ctattttaca gaaaaccgtc agacgtggat
     8161 tatacctttt cagcggacca gtcggatcag gcaagacttc tctaatgtat cggttggctt
     8221 tggaagaaaa attgcaagtg attactatcg aagatcctgt agagatagaa gaaccgtttt
     8281 ttctgcagct acagatcaat ccgaaaatag cacagactta tgatacactt ttgagactcg
     8341 cattacggca tcgtccagac ttgctaatca ttggcgagat aagagatgaa tcaactgcac
     8401 atgctgcggt aagagcagca ctaacagggc accgggtttt tgcaaccgta catgcacgca
     8461 atcttgaagg tacattggtc agaatccatg aactgacaaa gaaaaaagaa gagctatctg
     8521 aatgtttagc tggtgtcatt tatcaagaac ttttggaaga tgccaatcac catccttctg
     8581 ttttatggag ttatcaattt atggaggaag aatcagtaaa aaaatcatgg gaggaaagtt
     8641 atgaagaagc tcaggaaaat atttggcagt cagaactatt ctaaaggatt aacaagaagc
     8701 cagcaaaacc agtttgcaca aaccatggga gaactgcttt tatctggttt tagtctgcaa
     8761 gaatcccttt atttattact cacgatccgt tcgtttccac ctgttatttt ggcaaatatc
     8821 caacatcagc taaaggaagg gcaagcgttt agtgaggtac tttcgcaaat aggatatgga
     8881 gcagagcaac tggtacagat cgagttagct gaacgtcatg ggaatctgcc gacaactttg
     8941 ttggaaattg ccaaacagtt tcggcttgtt gaaggatttc gcaatgattt gaaaaagctg
     9001 cttgcatatc cgatgctatt gctcaccttt ttgctaggta tcttgtttag tatgcggttg
     9061 ttcgttttgc ctcagcttat tggttcagga atggtcatgc aagagcattg gggtattcag
     9121 ctgatacaag tctttcattg gtatgttcta ctattcatcc tagcggttac tctattggct
     9181 ttactagtaa ggtatcgtct gatgaagttg tctcctgttg atcagagtga atggataagc
     9241 agacaattat tgattggacc gatatattcc ttgtatcaat cggcttattt gtcactagag
     9301 attggaaagc tttttcatga aggtttcgaa ttaaggcaaa tcatagtctg cttaaaggaa
     9361 acgaaacaag aaagtctgat tcagcatctt gcctctaagt tgattcaagg attggaggca
     9421 ggtatgtcat tggctgagca gttccagaat tatcgttttc tatcagaaga attcagtaaa
     9481 attgttttac agggagaggc aaagggaagt ttaggtaaag aactgatttt ttacagcgaa
     9541 tggacaagaa aacagctttt caaacgtctt catcactgga tgcattttat tcagccaatc
     9601 atttttttgg gcgtagcggc tttgatttta ttagtttatg ccgctgtttt attacctgtt
     9661 tacggaagca tagaggaggt tttgccatga aaaagagaaa aatatcttat tatagcggtt
     9721 ttacattgat cgaaatgcta atcgtcttac tgatcatcag tgtattagtg ttgttgttcg
     9781 tccctaattt gtcgcgttat aggaatcatg tagaccaaga aagtcgtgaa gcgatcatcc
     9841 agttggtaga cacacaaaaa gagctctatg cgcttcaaaa taatggaaga gttcctactg
     9901 tggaggaatt gttgaatgaa ggctatatca aacgagaaca tgcagaaatc tatcagcggc
     9961 cttgaaggta aggttttagc atcaggatat acgttgtttg aatcactctt actgatttta
    10021 ttacttacaa cgatcctttc ttttccagtt ctcgcatttt ctgcatggaa gaatgaattg
    10081 gcagtctatc aatttttcgg ccaatttgaa aaacgaatct attctacgca aaaaatcgct
    10141 attgtgaatc agatccaaac aggcttttat tggaatagtg aagagaatca aatcattttt
    10201 catgttccga atccagataa aataaattgg gaagtattag atattcctga tgagattacc
    10261 ttgaaacgcc atgcttctat tacttttgct gctagaaccg ggaatgaaag ttcactaaaa
    10321 gcttatcaat tttattggga agaaaaagag cggacgatta cgtatcaatt ccagataggg
    10381 agtggtcgct atacaaaaag aattgaataa aacaggattt gttttgttgg agtcccttac
    10441 agcgcttatg atcgcttctg tctgtgtcta cttagtgagc tttggccagc tgtttttgct
    10501 tcaggcagaa aagaaaagtg gggaggaact gctatttatt cgaatgattt atgaagatat
    10561 caagacgcag cggatgtatc acaaaagacc agagcgaagg gaagaaatga tcccatacga
    10621 tatccaaatt gtcgataata atggagtaat cgaaaaagtg tttgtcaata tggagaaaga
    10681 agcgatcagt attgaaaaaa caagtaattc cagcatttac gctgattgaa tgtctaattg
    10741 gactgatgat attgagcagt ctattgctca cattcaatct tttgattcag caaaccatcc
    10801 aagtaagtga atttttgcag cgtaaagatc aaaaagagtg gctaatattt ttagcacaat
    10861 tagaagaaga actcaagagc agtcacaatg tctctgtgaa aaatcagcaa ctgcactaca
    10921 cgatcgagga taaacagtat atcatcgaaa gatatcaggc aatgatacgt aaaagaagaa
    10981 cttccggcgg acatcaacct atgttaacaa gtatcactga gcttcaatta ttggaaaaag
    11041 aacacacaat ttctttttat gtacactttg aaaatggaga agatggatat gccatatgga
    11101 caaaaaacga tcagtagaaa atgttataag ggctctattt tattttctgt cttgctttta
    11161 tttcttttat gtacgttttt attgatggca gcattagaaa gctaccgttt gtcaatggat
    11221 cttaatatac gaatcaaaaa ttattatata gcaaaaataa tgaagcagat ggctttatcg
    11281 gaacagtcta cgcttgccga aagttcggaa ggagtttttt actacacaac tggatctgta
    11341 acatatcagt gggtacaaca gtccctattc ttagaggtgg aagtctcacc gtttatcttt
    11401 cggttcacag aagaagtaaa aaatgataca gatactagta cagaatgatg gaatgttcgt
    11461 gaaaacgccc tcttttctgt tataacactg tttttaataa aaaaatgttt gccgtttcac
    11521 aaataaaaaa cagtgaaaac gattgaaatt ttgtcaattg ttcgatagaa ttaaacaggt
    11581 gaaaaaattg ttataatata tctagaaatg aggtgcacca tgttaccaga aaaaatggaa
    11641 caagggttta atcaaaacct ggaagctatt cagcttttac agaatgcatt gggaacttcc
    11701 tttttagaag cctatgtcga aaatgcagaa aatttaatag atgattatca agtacgtgtg
    11761 gtggatggag tgccgacaaa agagacaacg caacggatca cagctcttta tgaagagttg
    11821 aagaaacttt ctttcgagcc agaagaatgg cgcagactgt ctcaattact tttattgaag
    11881 ggcagccaga cagaacattt gcaggcgaac catcaactca ctcctgatag tatcggtttt
    11941 ttatttgttt ttcttattga acaactatac acaaataaaa aagcaccggt taagattctg
    12001 gatattgcgg caggaatggg caacttgtta ttaacagttc ttttaaacct atcgaatgcg
    12061 gggtaccaaa cagaaggaat tggtgtggat attgatgata cattgttggc tgtcgcagct
    12121 tctacaagtg atttgacaca agcaaatgtt caatactttc atcaagacgg gttgcaggaa
    12181 ttattaatag atcctgtcga ttttgcaatc agcgatttgc caatcggtta ttatccgaat
    12241 gacgaaaaag caaaagagtt tttaacaagt accgaagaag gccatagtta tgcacatcat
    12301 ttattgctag agcaatcaat gaaatatgtg aagccggatg gttttggttt gtttttgatg
    12361 ccgagtggat ttttagaaac ggatcaaagt gaagaaatca aaaaatggtt taaagaagaa
    12421 ggttatcttc aaggaatgat tcaattgcca gatgaattgt tccgtaataa acaatcgcaa
    12481 aaaagtattt tgatcttaca gaaaaaagga ccacaagcac aacaagtaaa agaagtcttg
    12541 cttgtgaaac ttgcttcatt aaaagaacca gagaaggtga cggaattttt caacgagttt
    12601 aaaaactgga aatcatctgc tctgtaatca ttcaacgaga aggagagtca tatgtctaaa
    12661 acaattgcaa tcaatgctgg gagttcaagt ttgaaatggc aattatatca aatgccagaa
    12721 gaaaccgtag ttgcgaaagg gatcgtcgaa cgtatcggtt tacaagattc tatttttacc
    12781 atcaagtacg gcgataatca aaaattcgaa caaatcatgg atatcgaaaa ccacgaagta
    12841 gcagtaaaaa tgctgttaga tcagttgatt gaactgcaaa ttttaagctc ttatgatgaa
    12901 atcaccggag tagggcaccg tgttgttcac ggaggagaaa cttatgggga ttcagtagtg
    12961 atcgatgaga aagtaatgga ccgcattgct gaattagcag aatttgctcc tttgcataat
    13021 cctgcaaact taatggggat caaagcgttt aaaaagattt tacctgacat tttgagtgtg
    13081 gctgtattcg atacatcttt ccactcaaca atgccaaagc aaaattattt atatagctta
    13141 ccggtgtctt attatgaaga tttcaaagta agaaaatatg gcttccatgg cacaagtcat
    13201 cgttatgtgt cggaacgtgc ggcaaaatta ttgaacaaac cgatcgaaga actaaaaatc
    13261 atcacttgcc acttaggaaa tggtgtatcg atcacagcag tcgatggagg taaatccatg
    13321 gatacttcta tgggattcac accattagct ggggtgacaa tgggtacacg ttcaggtgat
    13381 atcgatccag ctgttctccc atatttaatg gaaaaattag agattgatat tgatgaaatg
    13441 atcaatgttt tgaacaagaa atctggtctg ctaggcctaa caggattgtc tagtgatatg
    13501 cgtgatcttg aaaagaacta tgaaaaagaa catattcgtt tagcttatga cattttcgtg
    13561 gaccgcatcc gaaaatacat cggcggttat gtgacaacga tgaatggtgt tgatgcaatt
    13621 gtctttactg ctggtatcgg cgagaatgac ggacatgtac gtagtgaaat catcaaaggt
    13681 atgtcttggt ttggttgtga aatcgatcca gcgttgaatg aattgcgtgg tgaagaagta
    13741 gatatctcta caaaagaatc aaaagtaaga gtattagtga tcccaacaga tgaggaacta
    13801 atgattgccc gtgatgtgga gcgtctgcgc ggctaagcca aagcattcgt taggataaaa
    13861 gatgaaacgc atgcaagagg gaattcttgc atgtgttttt tttgcggaga agaccgcaaa
    13921 atggtaattt aacttcaatt tcccttcttt tttacaggat cttcttaggg aattttgctt
    13981 ggaaaagtgg tcaaaaggga aaagactggc attttattta ttgattttca ggaaaatccc
    14041 aatattttat tttcattcat ctttataagg gacattacat atgtaaaaag aaaaaacatt
    14101 tttagatcag cctcgtgggc tgtcgacttt attcttcgct ttatgtttag ctagaaaacc
    14161 gatccgaaac attcattgat cgaataataa aaacttgatt ttccacctgt actttgcctg
    14221 aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg cgaaaaaacg tcctagtcaa
    14281 tcagttggcc gagcggctgg cggctgatct gctcctgatc aataacgagt gcttcttgga
    14341 agtgttgctg caaaaagtag cggtcatggc tgactgctag gagcgttcct tgatagtctt
    14401 gcagcatttc ttcaatttct tctcgagcat agctgtcgag gtgattggtt ggttcatcca
    14461 aaatcaggac gttgattttt tctaaaaaga gtttcagcaa cgccaaacgg attttttcac
    14521 caccggataa atctttgatc cgtttaccca catcgctttg ataaaagcca cttttggcta
    14581 attgccgtcg agcgacttct tcgtttccag tcaatgtgat ggcgtaagcc agcaaacggc
    14641 tatcgggatc ggcaaactgc agctgctgcg gcaaataacc gatttgcaag ctactaccta
    14701 atttgatggt gccaccgtaa ggcttttcct caccgagtaa taatttgagt aaggttgatt
    14761 ttcctgcacc attgtcgccg atgatggcta aatgatctcc acgatagact gcaaaactgc
    14821 tgtctttgaa tagtacttga tcagcgtact gctggtgcaa gttttgaatg atgaaaactt
    14881 ctttgccaga ttttccacca ttagacaagc tttgcagccg atttttcggg ggaggtggcg
    14941 gtttgacgag tgtcagtttc gccaggcggc gttccagttc tttggctttt ttgaagaagg
    15001 actcattgtc tccttcatgt gcccactgac gaaaacgacg aatcatgact ttcaaccgct
    15061 ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc agccaagcga gcttctttca
    15121 agactcgaaa acggctgtag tttccgggat actcaatcaa tgcaccttct tccagttccc
    15181 atgtttttgt ggtgacttga tcgagaaatt gccgatcatg agaaacgacc agaaccgatt
    15241 tttttgtatt ctgtaaatag ttttccaacc agcgaatact gttaagatct aaatgattgg
    15301 tcggttcatc gagtaaaagt aaatcggcat cggctgctaa aattttggct aaagcgactt
    15361 taactcgttc gcctccgcta agttctgtta accgtgcttc ctgacggtgt ggaatcccta
    15421 agccttgcaa gatcgctgca atccggtctt ctaatacgta accgcctagt tcttcaaagc
    15481 gatgctgcag ctcaccgtaa cgggccagac acttctctaa gtttgtatct ggttctgtca
    15541 tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg tatctcagcg gcagaatgga
    15601 gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc ttgttcgacc gtcccgatct
    15661 ttaagccttt tttgcgactg ataacaccgc tgtcgacacc ttctctgccg gtcaagattt
    15721 gtaaaaaggt tgatttgcca gttccgttga taccgactaa ggcaattttt tcaccagctt
    15781 caatttgaca ggataaatcc tcaaataatg gattcccgcc gattgttttt ttaatagaaa
    15841 ttgcttgtat gatcatgatc tctctccttg agaccactca aaaaggccat ggttatccat
    15901 ggcctgtgtt atttaaactc agattatcat gagaaaaata ccttgagatg gccattgatt
    15961 ttacgtgaat tcaactgttt tggacaaact tgtcccgtca acagcaactg taaaaccaat
    16021 tgcatggcta aatgcgatat ggtatcgttg gtaattttca ttacttgtta ttttagtcat
    16081 gcgcatcata gtattttccc tccttcgtgt agttgtcttt tccagtgtac ctgaaagtgt
    16141 cagaaagttc aactagcgct gacatttact taatatttat cgatgtgtaa ttctgtcaga
    16201 gagctttttt tatctgactg agggtaatgt ctcgtggacc acatcattgt attctgagct
    16261 aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa aactttgtct acatgacttc
    16321 cttttgccat ttgcttaata ccgttgcatt aaacagctct tctctttgga aatctcacca
    16381 acatcgcgtt caggagtatc gttgtggttg caatggcaag cggtagcctt tcatcaattc
    16441 ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt acccgaaccc gaaataaaat
    16501 tttagtttgg cgataaaatc ggtatccgcc atcgatactt catatttttt cgtatcagat
    16561 tcatcggaaa cttccacatt gacgaaccaa taagctttgg catgcttagg tcgttttaaa
    16621 ccacaccagg gcgactaatc gccctggtgt tcactttttt ttattttact tgaactcatt
    16681 gactgttttc cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg
    16741 tttttatcac ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt
    16801 taagctttgg tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc
    16861 acatcgcaag ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct
    16921 acagcattca atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt
    16981 tctctggcaa taatttgttt tggtgagcca tattctacga cagaggttac attattcaag
    17041 ccagctttct tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct
    17101 tgttctgcaa gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca
    17161 tgtgctaata gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct
    17221 aattcagctt catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata
    17281 agcattcact cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa
    17341 aagagaatta tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg
    17401 gcagaaacaa taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa
    17461 gctgttaaaa tacccattac gcctaaaaca gcatatgcag ctccgtaaaa ttgtaggaat
    17521 ccttgatttt tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa
    17581 taatagctaa cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac
    17641 aaatcctcca aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat
    17701 tctttaaaag atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg
    17761 aattcaagca ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta
    17821 ccagcagtga cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa
    17881 gaggacaatt cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga
    17941 gcaatgagag agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt
    18001 tttccactct tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc
    18061 ttttggctga taatactgag cattcttcag tttatctggt aaatattgct gattgaccca
    18121 agcctgatca aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc
    18181 tttgtaatgg ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc
    18241 agcaagtgct gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc
    18301 gacgacgacg tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa
    18361 tacagcgttc actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc
    18421 gataaccatc agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag
    18481 ataatgaagt gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac
    18541 gtcataatga gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc
    18601 aatgatcgac agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc
    18661 cagttccagc ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag
    18721 aagagcatct tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa
    18781 agcatgttcg actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg
    18841 tgtacggctg cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat
    18901 caagatgatt cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa
    18961 tcggtgtact tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac
    19021 aacttgtaga tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt
    19081 agagccggcg atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat
    19141 catggaagag agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag
    19201 atgctgctga ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg
    19261 ttgcatagga ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag
    19321 atgcctagaa agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt
    19381 gtatgataaa gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga
    19441 ataaaacgac gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac
    19501 cagaaggaag attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg
    19561 aggccgccca tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac
    19621 caggtacgat ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc
    19681 cagaagggat tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc
    19741 aaatgagtaa aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat
    19801 tagtggaagc attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt
    19861 tacgcttagt ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg
    19921 gtatcccaaa caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc
    19981 cttatatctc tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa
    20041 atgaaaaaga aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac
    20101 tatgatgtag ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg
    20161 tttgcagaaa ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa
    20221 ccaattatcg aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat
    20281 gaacgcaatt atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta
    20341 gctgattctt tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt
    20401 gattttttgg atgaagacga tgacgaagct gtattcgagc ttcactggga taatcagcaa
    20461 ctagcagaat tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca
    20521 agttattaag gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa
    20581 gcagtcgaag cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa
    20641 gattctttag atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat
    20701 gattttacat cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc
    20761 ttaccggaaa tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta
    20821 gctattggaa aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc
    20881 tggaagaaat actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg
    20941 gaaaactacc agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca
    21001 tttggcacag gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg
    21061 cgtggtggtg aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca
    21121 aaatatcttg gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg
    21181 gcgaaagaaa atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat
    21241 cttttgacag gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc
    21301 atcttattga tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt
    21361 tcaggaatca ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt
    21421 atcgtggatc aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag
    21481 gtggaataga tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt
    21541 ttagaagggg aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa
    21601 gtttttttgg cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa
    21661 gaggctgttt atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg
    21721 attaccatag cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc
    21781 actgaattag gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg
    21841 gatgcaaaaa aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg
    21901 gaacaatccc atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta
    21961 tttgagcggc tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa
    22021 ggcgaaaaat caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta
    22081 gctgtctttg gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac
    22141 aaagggatac tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat
    22201 ttattaagtg ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct
    22261 tcattaataa aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata
    22321 gaaacaatta tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa
    22381 catttccgta aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt
    22441 gtcccgattc ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga
    22501 aattttaacg ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt
    22561 agcatttgtt gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa
    22621 atcaggcgaa ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa
    22681 aatggatccc catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat
    22741 cactttagaa gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt
    22801 aaccaaatta gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg
    22861 aaagatgctg ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag
    22921 actacataac atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca
    22981 ggaaacttta gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg
    23041 ggaattagaa gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca
    23101 cttgatgcag acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat
    23161 tcgaatagcg acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat
    23221 ttattcaatc tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt
    23281 gctagctatt cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat
    23341 ccacacaaaa tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc
    23401 taatatgtac caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat
    23461 tcaaatccga acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc
    23521 ctataaagaa ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg
    23581 gttccatgag atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg
    23641 cgtaaaagga gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac
    23701 tgagttacca aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg
    23761 taacaaaacc actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa
    23821 aaacggagat atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg
    23881 gttgaaatta gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca
    23941 agatcgagaa gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct
    24001 aggatttacg cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt
    24061 taattatcaa acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt
    24121 gacgatggcc aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa
    24181 gcaagaagca gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg
    24241 tcatgaaggt ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg
    24301 ctgtaatccg attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc
    24361 cattcatcgt cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt
    24421 gattgaagtc gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga
    24481 aatttacggc tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct
    24541 aacgaaaaat ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca
    24601 tttgacggtt ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc
    24661 agtacctgat gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag
    24721 ttgttcaacg ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg
    24781 gctacggcct acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact
    24841 atctggtaaa aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata
    24901 ttagtgtaga agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg
    24961 atacgaaaaa aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc
    25021 ctttatacga atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac
    25081 aattcggagc agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg
    25141 attctaaaaa caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct
    25201 taagttagac tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt
    25261 tgtcagcagt cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt
    25321 gctgaagatg aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta
    25381 tcaaaaagga tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa
    25441 agcaaagatt ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt
    25501 ttatgatcca aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact
    25561 ttttggtcaa ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat
    25621 ttttatggat ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa
    25681 tctgacacaa gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat
    25741 ggttttcttt acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga
    25801 tgaggtcgaa aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc
    25861 gattgatcac ataaaaggtg tcgaaaatcg caaaaaatca gaaaaatggg cgcaaggctt
    25921 gattgaagaa gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt
    25981 tgccaatgcc acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt
    26041 attgaacatc atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc
    26101 acttttcagt agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga
    26161 tccgtttatt caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc
    26221 gctgaaagaa gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga
    26281 tttatatgga acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa
    26341 acggtatgta gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca
    26401 aggcggggga aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag
    26461 tctacgtgtg ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga
    26521 ttggacgatt ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa
    26581 gaattagatt agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt
    26641 ttttcatatt actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca
    26701 atggtttgat aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact
    26761 tgctctggac gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa
    26821 gctaaaacaa gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg
    26881 acagcggtta aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga
    26941 tctccttcac caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa
    27001 gcaagaggaa aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct
    27061 ttttcagcct gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct
    27121 gcattcgctt ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt
    27181 cctgcttctt ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc
    27241 ggaaagtgga tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc
    27301 accgattgtt tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga
    27361 gcagcttttg tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg
    27421 ccaatcagtt cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc
    27481 ccgctattca aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa
    27541 agattatgtc ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa
    27601 tgtccgattc ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat
    27661 accaaaaagc tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt
    27721 gagactggtt tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt
    27781 tttgaaactt tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct
    27841 aaagacactc tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag
    27901 aggaaagaga aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt
    27961 catgatgaac aagggtgatt tttttattta catagctaag aaagggagag aaaactatga
    28021 gtttccaaag accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt
    28081 ttgtcgaaga aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac
    28141 cgatttttga acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt
    28201 caaaagagat gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag
    28261 gtacagcacc aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga
    28321 aaccgttcaa aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac
    28381 ggttacgtca attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg
    28441 atgtggagag catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc
    28501 ggctagtcat caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga
    28561 tcgactatct tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg
    28621 aaaatccatt acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag
    28681 ctccatctat cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt
    28741 ccatgctgga tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag
    28801 attattacac acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag
    28861 caacgatttg cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa
    28921 cacctggctt cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg
    28981 aagtagttat ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa
    29041 caaatatcga agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc
    29101 gcgacttcat gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca
    29161 aattagtatt ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa
    29221 tggcaagccg aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag
    29281 tctatgatga aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat
    29341 ctattgcgga ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt
    29401 acaaaaaaga cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat
    29461 cgttcaaatc gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg
    29521 tcgcagtgaa tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt
    29581 aaatcctaaa atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa
    29641 tcaagcaaaa accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg
    29701 tttgaaatat cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag
    29761 aaatgcgaca acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga
    29821 aacaccttac ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg
    29881 tgtccatgct ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct
    29941 gatgaatgct ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt
    30001 acgtggagac cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc
    30061 agaagaaatc caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg
    30121 tatcgaagtc acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg
    30181 cagcgacaaa ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa
    30241 agaagtagaa ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt
    30301 gaatgctaaa ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata
    30361 tgtaggacaa ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa
    30421 aggtccaatt gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc
    30481 aaaaccaggt gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt
    30541 aggtgctatc cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa
    30601 tttcttatgg gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt
    30661 ctctgctcac catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga
    30721 ccctgcaaaa gtctatgcag aagcttatga tatcgtcttg aacggttatg aactaggcgg
    30781 aggttctatc cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt
    30841 tacaaaagaa caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt
    30901 ccctccacat ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga
    30961 agataatatt cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag
    31021 tgatgcacca agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa
    31081 tatagaagaa tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa
    31141 ccttgattta attgaaaaac actttataat gaagccataa catataaagc ggggcttttc
    31201 catgaaaaaa acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc
    31261 tatcgtttat gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat
    31321 ttattcaagt ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat
    31381 aggattcgaa gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt
    31441 ggcgtggtta aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac
    31501 ctccctttgt atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc
    31561 gttgtttggg ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc
    31621 atcgggcggt ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg
    31681 ttctatttcg atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg
    31741 gcaatatatg ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt
    31801 ttatactaaa caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt
    31861 aaatgaaatc caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc
    31921 ttataagcat gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct
    31981 acttagagaa gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt
    32041 ccagatactt ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg
    32101 aaatactgct gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca
    32161 gcaaagaaaa actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat
    32221 ttttttaaat ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc
    32281 tattttcact taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt
    32341 gaatggaaaa acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc
    32401 gttcattagc tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg
    32461 gctatttggg cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc
    32521 ggcaggaatg ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga
    32581 agagactcta tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt
    32641 ttcagcgagg gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca
    32701 acaactggtg gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac
    32761 cgtttacgca tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct
    32821 tgtttgtatt gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag
    32881 gtacatcctt cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa
    32941 cccgtgttta ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg
    33001 cgtttgtttg gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc
    33061 tgaatatcgc tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg
    33121 atcattacaa tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc
    33181 tagttgcagt gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat
    33241 ttacttggat tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt
    33301 tttatgaagc aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa
    33361 tcacattgat tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac
    33421 gttttgatta tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca
    33481 gtaagagcaa accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt
    33541 tcgttacggc accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg
    33601 ctgaaagtaa aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg
    33661 ttgaccaagc gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa
    33721 aattagaact aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag
    33781 aactttatcc aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac
    33841 cgtacattga aaaattaaag gaacaagatt tgttgaaaga cactgttctg atcggactag
    33901 gaacgaatgg ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc
    33961 gaaaagtata ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg
    34021 cgatgcttga acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct
    34081 acagtaatga ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga
    34141 tgacacatta cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca
    34201 gcagtgagac aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt
    34261 tttgcctgaa gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca
    34321 aaacggttga gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg
    34381 cagcttctgc ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa
    34441 caggtcttct tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta
    34501 atcgtctaga aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata
    34561 cgtaaaaata atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca
    34621 tccatattgt aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg
    34681 attccttcaa ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc
    34741 cttttaggtg caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga
    34801 cgatggacca tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact
    34861 tcttctaacg tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt
    34921 ttatttgttg gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta
    34981 tgtcgaacga cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt
    35041 aaatggttaa gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata
    35101 gcgtcgatag ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca
    35161 caaaggtcaa caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca
    35221 tctaaagaaa aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat
    35281 tggtcgattc gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt
    35341 ctgtattata ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta
    35401 tgcatactat gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg
    35461 ggtagttcga gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt
    35521 tttaccgcaa gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt
    35581 atatctggcg gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt
    35641 caaaaatcca gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga
    35701 aaatccagga ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag
    35761 tttgatcatg tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt
    35821 gatttccaga atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact
    35881 tggggatctg ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc
    35941 agatatcaag aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc
    36001 ttttgacaaa ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata
    36061 aaatgataag tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt
    36121 tttcaatgaa aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa
    36181 aatagaagac aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt
    36241 tgaggtgatt gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa
    36301 ggttatgact tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa
    36361 gaagaaaaag tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct
    36421 gttgatgccg tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc
    36481 ccgatttttg ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca
    36541 tggttcagtt attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc
    36601 gagtttaaaa acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta
    36661 tattttgaag atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa
    36721 atgcaggagc tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg
    36781 ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct
    36841 ggtgaactac ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct
    36901 ttgagacagg ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc
    36961 aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg
    37021 gagatcgaac ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat
    37081 cctgcctttt atcagtccgt caatcgggag aattacatct atatctatct cactgataga
    37141 acgatcatcc cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt
    37201 gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa
    37261 gatcctcaaa aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca
    37321 aaccaagaaa aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga
    37381 atgaaaccga aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat
    37441 ttttcacgtt tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa
    37501 acgacgattt ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc
    37561 tctggaaaag agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt
    37621 tcttttgaac atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca
    37681 aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat
    37741 gggaaaggaa aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga
    37801 gaactgatga atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa
    37861 ttccgtaatt ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc
    37921 ggtactcaat ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa
    37981 caaaaattgg atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc
    38041 aaagaagagg aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag
    38101 gcaatactcg aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa
    38161 caaaagcaac tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa
    38221 aaaacgatat taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa
    38281 aagaaacgat ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc
    38341 ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag
    38401 aaactagcga tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta
    38461 cgccgacaaa caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg
    38521 ccaattgtcg aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg
    38581 gataaagaag aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga
    38641 actcgaattc ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct
    38701 tttggacagt taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa
    38761 gacactgggt cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca
    38821 aaaaatagtc agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg
    38881 gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt
    38941 tgtggatcaa cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat
    39001 atcttaaaag tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa
    39061 atcattgcca aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa
    39121 caaaaaatca tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct
    39181 ttgaaaaagg catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc
    39241 gaacaatctt tagatagacg aaaaagcaag atggccaatg caagaataga gctggaagaa
    39301 ataagacaac aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca
    39361 gaaaaaaata atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg
    39421 caagatacgg atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa
    39481 gaagactcga atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt
    39541 catacgcttc ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa
    39601 atagaaaagg aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca
    39661 attgaagaaa tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca
    39721 gttacttctt atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat
    39781 atccagctaa ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata
    39841 agacgtatga tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt
    39901 aatcaaaaaa tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat
    39961 gagcttacgc aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact
    40021 agtttggaac ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat
    40081 catctccaac gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt
    40141 tatcgaggga aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga
    40201 agtgcgcata ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct
    40261 cttgccgatg tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat
    40321 gaaggatttg ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg
    40381 atcgaaagtg aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga
    40441 gtattacaac aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa
    40501 cttggataaa aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca
    40561 cgtggacgtc aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa
    40621 aacgtgtggc atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca
    40681 gcaaaagaga tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat
    40741 acagttgctg tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac
    40801 cagccaatgc tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt
    40861 tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa
    40921 gtagaaacga ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg
    40981 cgtatcggtt cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt
    41041 ttacaagctt acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta
    41101 gataaacggg cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct
    41161 cagacgcatc aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga
    41221 tatctgcttc tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca
    41281 cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa tacaattttc cgatcaaaac
    41341 aaacctttat cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat
    41401 gattttgatt ttttctttat gcattatcag tggggagtta tccaagggac attttattct
    41461 ctgacgaaat atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag
    41521 aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct
    41581 ctgctgtcag agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat
    41641 atccccgtcg aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc
    41701 gatttcgtct atggcgaagt catctattcg actgacccga agcatgaggt ctcgacagaa
    41761 caggcttttc aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga
    41821 atgtatcgct accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa
    41881 ctatatgcct ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt
    41941 ggaagaaaat tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta
    42001 accgagaccg gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa
    42061 atcgatcatg tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt
    42121 caggttcttt cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta
    42181 cgggaatcga tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc
    42241 attcaaaatc aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta
    42301 caagatctca ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg
    42361 atgagagatt atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt
    42421 ggcggtattt tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta
    42481 ctttcggaaa aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta
    42541 atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc
    42601 aatgggaata aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact
    42661 tcttatgcca gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg
    42721 attcttgatg aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga
    42781 gaattagctg tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa
    42841 gaattgtggt cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc
    42901 agggaacttg ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat
    42961 aaacaaactg tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt
    43021 acagaagaac aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca
    43081 tcaatggatt ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga
    43141 ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc
    43201 aagctcgaac aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg
    43261 ctgttttctc aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg
    43321 atctcgacat tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac
    43381 gcatttaatg ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga
    43441 ttgaatctca cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt
    43501 gaagaacaag ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg
    43561 cgtatgatct ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc
    43621 gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa
    43681 gacatccgca tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt
    43741 cagtaatggg taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc
    43801 aaggaggctg ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt
    43861 ttaaaggtta tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct
    43921 cgatatcgca aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt
    43981 gccagttagc actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg
    44041 tctcttctgg aagcatatga aattctttct cagctagctg aaaaatctct ttttctggtt
    44101 tttggaaacc tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt
    44161 gaagttgctt cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg
    44221 ggacattctt ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat
    44281 tatcaagttc ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag
    44341 ggtagtctaa atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc
    44401 aatctcctgc cattactttt gggaagtttt catcactatg atggcggaac cggatatata
    44461 gtggatgcat atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg
    44521 gctgctgctg atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt
    44581 tacacgcctt tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa
    44641 attgtctaaa aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag
    44701 aataaggttt ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt
    44761 gatgaacaaa ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg
    44821 gaattttcga aagatttatt tttttaatac agtctcaata taactattca aacataaaat
    44881 aacgttattt gatttatttg tttatagtaa attttattta gtaatcattg ctttaaaata
    44941 ctacgcttat ataacagaga aaataagcgt tataataaag aacagtatta tttaaaatga
    45001 cagaaaacat tactattaaa gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat
    45061 ttagcttttg agataaaata atagtacagt tttatacgct gtttcatatg tttagaaaat
    45121 ttgaattaga tttgaaaact ctaattttta aatgataaaa aaagcgagaa tgctggtttt
    45181 actgtatatt aaccttagat aactttcatt tttttaaaaa aattatgaaa aaaaggtagc
    45241 tatttaaaag ttgattatgt ataatagaaa cagatgggag attattatga tcttctgtta
    45301 caaaaaagtg attcttatca cgaaaaggag tggtgcacgt tgttaacatt atatacttcc
    45361 ccaagttgta cttcctgcag aaaggctcgt gcgtggggtt gcaagaacat cagattccat
    45421 ttgttgaacg taatattttt tcagaaccat tgaacagttc tgaattaaaa gcgattttac
    45481 aaatgacaga agacggaaca gaagaaatca tctcgacacg ttccaaagta ttccaaaaac
    45541 tgaacatgga tttagatgat ctgccgttac aagaattatt ggaacttgtg caaaacaatc
    45601 ctggattgct tcgccgtcca atcatgattg atgataaacg tctgcaagtt ggttttaatg
    45661 aagatgaaat tcgtcggttc ctaccgcgcg atgtacgaca attagaatta cgtcaagcac
    45721 aattgatggc tggattatag aaggataata cgaaacttgg aaaagactat tacaaagata
    45781 gtttttccaa gtttttttgt ttttacaaaa agtattcttg attagatgta taaaaaggta
    45841 gtagaagatg atgattgcac gaaaaataga gagggaaaat tagctgaaaa gtcggctata
    45901 tagtattcgt ctgccaaaaa tgttatagtt aaataagata gcttgaaagg ttgaaaaatc
    45961 cgctggtttt attaaattcg cttaacagcg agattttttt tctttacatt taagctgatt
    46021 tcgctacaat gaatctatat taaacattgt ggcaaagtga ggtgtagcaa aatggaaatg
    46081 gaacatatta atgaaaatac gatccgtgta ctaattggtc atgaagattt agaggaacgg
    46141 ggcgtttcat ttttggattt acttggcaat cacaaagaaa tcgaaaattt cttttatagt
    46201 attttggaag aagttgatat cgaagaggag ttccgaggaa gcgaagctgt cactttccaa
    46261 gttttgccaa aaggagacgg attagaactg tttatcagta aaaatcttcc ggatgaagaa
    46321 atttctcaat cagaagattc tactgttgat tcagaagatg tgagtgactt tcttcgtcag
    46381 cagattattg gcagcgatcc ggaggaagaa ttatctggaa gcgccctaga agaacaggaa
    46441 agacaagcaa cttttgcttt tgaagatttt gaacaagtga tccaacttgc aacagatgtc
    46501 gaactagagt ctgcttggac tgatttgtat ttattcgaag agcgctatta cctagtcgtt
    46561 cacttctgga tggaaaatct caaccaagcg gatgtagaga atcagattgc tcgaattttg
    46621 gagtttgctg aaaagagcga tcgtacacca gaaacattga atgaatatgg acaatgtctg
    46681 atggcgcgta atgctatcga gcgaacgaaa ttttattttg tataaatcag cattatagta
    46741 agagactgtg acaaaaattg tgtcgcagtc tttttttcgt ataaatgtgt tcaaaagctt
    46801 gcatctgcgg taacaagccc aaccaaacga aaaatattgg gaactatttt tgtttagctg
    46861 ttcttattac ttgatgcaga atagcttttt cacgccctct tttttcgtgt aaataaattc
    46921 ttagacgtaa ataagttcag agggagaaaa atgctgcttg caatgaatga atataataag
    46981 tgctgttttg ctaaacatgc agaaaaagga aagaaatatt attgtcgtgg ctgtcgaaaa
    47041 agagtgattt tgaaaaaggg aaagaaaaaa tgtgctcatt ttgcccatca aaaatcagat
    47101 aactgttcgg tctttagtga aagagaatca aaagagcact tacagttaaa ggaatgcttc
    47161 atggattggc tgggacagtc agtagaacca gtatttttgg aagcctattt gccgaggttg
    47221 cggcaaaggc cggatatttt gttggcgaat ctagcaatag aaatccaatg ttctagactg
    47281 tctcaccaga gatttattga aagaacccaa aattatttga ataacagtta ccaagtatgg
    47341 tggatacttg gtaattcttt tcttggacaa tcgcaattct cattgataga aaaaagttgc
    47401 tgctactata atagaaaaag aggagtgcat tgctggaaag ctgatctcaa gaaacaaaag
    47461 ctttatttgt atcatcatat tacagagact gtatcagggc atatttcctt ttgcagtagc
    47521 tgctggactt tttctagtag ggaccttaaa gaaatattca ctagtaacga aattaaaatg
    47581 aaccaaatga aaaaaataga aaaagtgagt gaagatggga agaaatggct gacacgccaa
    47641 cttattcaca agcaaaagaa tacagtatcc atacaagaac aatgctactt gcgtcataaa
    47701 catcttttgc atctatcatc gtggatctat caaaaaagtc ggtttttctt ttatttacaa
    47761 gaacaggttt ttctttatcg aatgttgtac gaagaagcct tggaaaacca gaaagttcct
    47821 gatttcaact catggctttg ccaaataaaa gaacataaaa gggaatggct ttttcctatg
    47881 attgatgaag agatggtcta tcagcagttt tttaacgaat gcatacactt gtcttctttg
    47941 aaaaaatagt aagtataaaa ataggctagg aatcggtttt ctatgtaaaa caagatttag
    48001 ttatgctaaa attgatggaa gcagacacat gaatgagtag aaaagttaga tgctgtgaca
    48061 tgtggatgtt agactatgat ctattttgat agatagagct aagggaagaa aaggagtgga
    48121 tttcatggaa gtaaagcagt tgccaaaacg agaagaattg cctgaaaatt taacttggga
    48181 cttgaccaag atcttttcaa gcgaccaaga gtttgatgag aaatatttgg aattatcaga
    48241 agagttaaaa caatctgaaa aacacaaagg aacacttgat caaggcgctt ctcaattttt
    48301 aaatgcgatt gaattcgtat tgagggttta tcgccaaact gaagtcattt atgtatatgc
    48361 gcaccttaaa aacgatcaag acactggaaa tacagattac caagcgcttt atgcaagagc
    48421 aagcagtctg ttttcgaaag ttagtgaagc cgtttcctgg tttgaaccag aaatattgca
    48481 attgtcagat gaccagattt ggcaatattt caaagaagaa ccaaaattgg aagtctatcg
    48541 ccattatatc cagcaaatag tagataatcg agcccatgtc ttatctgctg agcaggaatc
    48601 tcttcttgct ggagcaggtg aaatctttga tgcttcaagt gatacatttg ctgttttgaa
    48661 taatgcagat ctagtttttc caacgattga aggagaaaat ggtgaaatag tccaattatc
    48721 tcatggcgtg tatggtcagt tgctagaaag cacggatcga agggtgcgcg aagcagcatt
    48781 taaggggttg tacagtgttt acgaacaatt tagaaataca tttgcttcta ctttaggcac
    48841 acatataaaa ggacataatt ttaaagcgaa agtccgtaat tacagctctg ccagagaagc
    48901 gtctttgagc aataatcata ttcctgaaag tgtatacgat actttggtag acgtggtaaa
    48961 caagcatttg cctttgttac atcgatacat ggaattacgg aaacgtttat tagaagtgga
    49021 aaaactgcac atgtatgatc tttatacacc ggtcttaggg gaagctccaa ttacctttac
    49081 gtacgaagaa gcaaaagaaa aagctttaga agcactgaaa ccaatgggtg aagaatacat
    49141 ggccatcgta gaaaaagcat tctctgaacg ttggatcgat gttgtcgaaa ataaagggaa
    49201 acgaagcggt gcttattctt cgggaagcta tgacacaaat ccatatattt tattgaattg
    49261 gcatgatacg ctggatcagc tatttacgct tgtccacgaa atgggacata gtgttcatag
    49321 ttatttcact cgttcgaacc agccttatgt gtacggcgac tactccatct ttttagcaga
    49381 aattgcttcg acaacgaatg aaaatatcct aacggagtat ttattggaaa cagaaaaaga
    49441 ccctcgtgta cgggcttatg tactcaacca ttacttggat gggtttaaag gaacagtttt
    49501 ccgccagaca caattcgctg aattcgagca tttcatgcat acggaagatg aaaaaggtgt
    49561 gccattgact agtgaatacc taagtgatag ttatggtaaa ttgaatgcaa aatattatgg
    49621 tccagcagtc gaagaagacc cagaaattaa attcgaatgg tcacggatac cgcattttta
    49681 ttataattac tatgttttcc aatactcgac tggcttttct gctgcttcag cactggcgaa
    49741 gaaaatacta aaccaggaac cagaggcact agagaactac ttggcttacc taaaagcagg
    49801 taacagcgat tatcctgtgg aagtaatgaa aaaagcagga gttgatatga cacaggctgc
    49861 atatattgaa gatgcaatgt cgatgtttga acaacgtcta aatgaattag aagaattgat
    49921 cgatcgttta taaggctttc tatttttgat tttctcgaat agaaacgaaa aaaatacgtc
    49981 ggctctcttg taagggagtc gacgtatttt tt
//
LOCUS       DAFEZB010000013        49887 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFEZB010000013
VERSION     DAFEZB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.013"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LP859_000799"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LP859_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL3028772.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="LP859_000800"
     CDS             complement(322..891)
                     /locus_tag="LP859_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL3028773.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="LP859_000801"
     CDS             1077..1979
                     /locus_tag="LP859_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3028774.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LP859_000802"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LP859_000802"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL3028775.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LP859_000803"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LP859_000803"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL3028776.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LP859_000804"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LP859_000804"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL3028777.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="LP859_000805"
     CDS             8547..8732
                     /locus_tag="LP859_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL3028778.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="LP859_000806"
     CDS             complement(8794..9990)
                     /locus_tag="LP859_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL3028779.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="LP859_000807"
     CDS             complement(10160..11596)
                     /locus_tag="LP859_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL3028780.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="LP859_000808"
     CDS             11889..12698
                     /locus_tag="LP859_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3028781.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="LP859_000809"
     CDS             complement(12884..15037)
                     /locus_tag="LP859_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL3028782.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15331..16179)
                     /locus_tag="LP859_000810"
     CDS             complement(15331..16179)
                     /locus_tag="LP859_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3028783.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="LP859_000811"
     CDS             complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="LP859_000811"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL3028784.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVTYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18680..18859)
                     /locus_tag="LP859_000812"
     CDS             complement(18680..18859)
                     /locus_tag="LP859_000812"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL3028785.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="LP859_000813"
     CDS             complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="LP859_000813"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL3028786.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21007..23286
                     /locus_tag="LP859_000814"
     CDS             21007..23286
                     /locus_tag="LP859_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3028787.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23379..24026)
                     /locus_tag="LP859_000815"
     CDS             complement(23379..24026)
                     /locus_tag="LP859_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028788.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24205..24924)
                     /locus_tag="LP859_000816"
     CDS             complement(24205..24924)
                     /locus_tag="LP859_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3028789.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25055..26206)
                     /locus_tag="LP859_000817"
     CDS             complement(25055..26206)
                     /locus_tag="LP859_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL3028790.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26423..27163)
                     /locus_tag="LP859_000818"
     CDS             complement(26423..27163)
                     /locus_tag="LP859_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3028791.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="LP859_000819"
     CDS             complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="LP859_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL3028792.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27547..28140)
                     /gene="yqeK"
                     /locus_tag="LP859_000820"
     CDS             complement(27547..28140)
                     /gene="yqeK"
                     /locus_tag="LP859_000820"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL3028793.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28124..28774)
                     /locus_tag="LP859_000821"
     CDS             complement(28124..28774)
                     /locus_tag="LP859_000821"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL3028794.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="LP859_000822"
     CDS             complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="LP859_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL3028795.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="LP859_000823"
     CDS             complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="LP859_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL3028796.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30217..30744)
                     /locus_tag="LP859_000824"
     CDS             complement(30217..30744)
                     /locus_tag="LP859_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL3028797.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30924..31709)
                     /locus_tag="LP859_000825"
     CDS             complement(30924..31709)
                     /locus_tag="LP859_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL3028798.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31702..32571)
                     /locus_tag="LP859_000826"
     CDS             complement(31702..32571)
                     /locus_tag="LP859_000826"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL3028799.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32572..33951)
                     /locus_tag="LP859_000827"
     CDS             complement(32572..33951)
                     /locus_tag="LP859_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL3028800.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="LP859_000828"
     CDS             complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="LP859_000828"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL3028801.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34403..34879)
                     /gene="accB"
                     /locus_tag="LP859_000829"
     CDS             complement(34403..34879)
                     /gene="accB"
                     /locus_tag="LP859_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL3028802.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="LP859_000830"
     CDS             complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="LP859_000830"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL3028803.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="LP859_000831"
     CDS             complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="LP859_000831"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL3028804.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="LP859_000832"
     CDS             complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="LP859_000832"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL3028805.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37909..38133)
                     /locus_tag="LP859_000833"
     CDS             complement(37909..38133)
                     /locus_tag="LP859_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL3028806.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38186..39148)
                     /locus_tag="LP859_000834"
     CDS             complement(38186..39148)
                     /locus_tag="LP859_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL3028807.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39158..39598)
                     /locus_tag="LP859_000835"
     CDS             complement(39158..39598)
                     /locus_tag="LP859_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3028808.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39904..40869
                     /locus_tag="LP859_000836"
     CDS             39904..40869
                     /locus_tag="LP859_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL3028809.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40926..41156)
                     /locus_tag="LP859_000837"
     CDS             complement(40926..41156)
                     /locus_tag="LP859_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL3028810.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41242..42129)
                     /locus_tag="LP859_000838"
     CDS             complement(41242..42129)
                     /locus_tag="LP859_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL3028811.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42218..43441)
                     /locus_tag="LP859_000839"
     CDS             complement(42218..43441)
                     /locus_tag="LP859_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3028812.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43458..44504)
                     /locus_tag="LP859_000840"
     CDS             complement(43458..44504)
                     /locus_tag="LP859_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3028813.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(44668..46395)
                     /gene="ptsP"
                     /locus_tag="LP859_000841"
     CDS             complement(44668..46395)
                     /gene="ptsP"
                     /locus_tag="LP859_000841"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL3028814.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46395..46661)
                     /locus_tag="LP859_000842"
     CDS             complement(46395..46661)
                     /locus_tag="LP859_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL3028815.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47140..49374
                     /locus_tag="LP859_000843"
     CDS             47140..49374
                     /locus_tag="LP859_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3028816.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49522..49842
                     /locus_tag="LP859_000844"
     CDS             49522..49842
                     /locus_tag="LP859_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL3028817.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14253 a        10708 c         8730 g        16196 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag
    15181 ttacatatcc aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc
    15241 ttaagtcgtg aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct
    15301 aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat
    15361 tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata
    15421 aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt
    15481 cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt
    15541 atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa
    15601 tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc
    15661 attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc
    15721 gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata
    15781 taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc
    15841 tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat
    15901 agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag
    15961 ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa
    16021 acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt
    16081 agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata
    16141 atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat
    16201 gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa
    16261 acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag attctattga
    16321 gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac
    16381 ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca
    16441 tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta
    16501 cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt
    16561 gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac
    16621 gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa
    16681 cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt
    16741 atacttctgt taaataagtg acaaaacgtt ccattgtaga aacaattccg cggtggataa
    16801 ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg
    16861 gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga
    16921 cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta
    16981 gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat
    17041 catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg
    17101 taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt
    17161 tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt
    17221 gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac
    17281 gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc
    17341 agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt
    17401 cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg
    17461 gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg
    17521 tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg
    17581 aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt
    17641 cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac
    17701 gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact gataataatt
    17761 taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt
    17821 cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt
    17881 tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga
    17941 tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg
    18001 cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc
    18061 caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg
    18121 ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg
    18181 catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag
    18241 ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact
    18301 caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga
    18361 aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa
    18421 aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc
    18481 tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc
    18541 tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa
    18601 attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa
    18661 atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt
    18721 tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag
    18781 cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct tctactaatt
    18841 cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga
    18901 ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt
    18961 gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat
    19021 aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca
    19081 atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct
    19141 acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa
    19201 ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg
    19261 ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt
    19321 ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt
    19381 tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt
    19441 agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct
    19501 gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct
    19561 gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct
    19621 tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc
    19681 actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt
    19741 agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg
    19801 gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt
    19861 gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca
    19921 atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt
    19981 aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa
    20041 gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg
    20101 actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg
    20161 atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc
    20221 gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga
    20281 ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca
    20341 tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta
    20401 tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct
    20461 ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt
    20521 gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg
    20581 atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat
    20641 gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa
    20701 gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag
    20761 aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact
    20821 ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat
    20881 cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt
    20941 accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa
    21001 catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc
    21061 cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat
    21121 tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa
    21181 tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa
    21241 gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt
    21301 atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga
    21361 gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat
    21421 gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg
    21481 cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg
    21541 gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg
    21601 aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt
    21661 aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt
    21721 attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt
    21781 tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc
    21841 ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta
    21901 ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg
    21961 atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac
    22021 tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg
    22081 attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta
    22141 ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa
    22201 tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt
    22261 gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt
    22321 aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa
    22381 caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc
    22441 gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg
    22501 cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt
    22561 acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg
    22621 gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa
    22681 aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa
    22741 gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct
    22801 aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct
    22861 ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga
    22921 acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa
    22981 tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta
    23041 aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt
    23101 ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa
    23161 gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc
    23221 tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta
    23281 ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata
    23341 ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa
    23401 tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt
    23461 tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat
    23521 cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag
    23581 ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta
    23641 ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc
    23701 aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga
    23761 aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc
    23821 attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt accgacatag
    23881 tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa
    23941 tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa
    24001 ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat
    24061 catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa
    24121 tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga
    24181 atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg
    24241 tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg
    24301 agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat
    24361 tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat
    24421 gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc
    24481 aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta
    24541 agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc
    24601 tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg
    24661 tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc
    24721 gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa
    24781 acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc
    24841 aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac
    24901 gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa
    24961 cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt
    25021 tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga
    25081 aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt
    25141 ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt
    25201 ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt
    25261 ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc
    25321 gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag
    25381 ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca
    25441 gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag
    25501 tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc
    25561 tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga
    25621 tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga
    25681 catacttggc attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat
    25741 caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat
    25801 acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa
    25861 agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt
    25921 cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa
    25981 ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg
    26041 cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta
    26101 cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc
    26161 cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc
    26221 atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata
    26281 cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc
    26341 aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg
    26401 ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt
    26461 tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt
    26521 tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt
    26581 cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga
    26641 tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat
    26701 aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata
    26761 aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt
    26821 gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga
    26881 tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg
    26941 ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc
    27001 catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt
    27061 ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt
    27121 ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt
    27181 cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt
    27241 ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc
    27301 cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt
    27361 cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg
    27421 ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg
    27481 ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg
    27541 agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg
    27601 ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc
    27661 tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc
    27721 aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt
    27781 atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa
    27841 cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa
    27901 accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg
    27961 tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac
    28021 ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat
    28081 tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt ccatattcat
    28141 acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac
    28201 agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta
    28261 cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt
    28321 tgaccataga agtcagctca tcgattttat accattttgg caaatattct accatatccc
    28381 caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat
    28441 cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat
    28501 tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct
    28561 catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc
    28621 ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca
    28681 agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg
    28741 aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt
    28801 cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc
    28861 caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg
    28921 caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt
    28981 cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct
    29041 gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt
    29101 tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc
    29161 ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg
    29221 ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag
    29281 gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca
    29341 gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc
    29401 cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct
    29461 gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta
    29521 agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt
    29581 catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta
    29641 catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat
    29701 ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata
    29761 gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc
    29821 ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag
    29881 gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta
    29941 gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat
    30001 ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt
    30061 gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta
    30121 aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta
    30181 taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc
    30241 catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa
    30301 ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc
    30361 acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc
    30421 tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg
    30481 tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt
    30541 tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc
    30601 ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc
    30661 tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca
    30721 tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat
    30781 aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc
    30841 gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag
    30901 aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt
    30961 aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata
    31021 atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga
    31081 tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct
    31141 gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca
    31201 taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca
    31261 cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc
    31321 agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca
    31381 cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt
    31441 cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc
    31501 tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg
    31561 attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa
    31621 gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac
    31681 tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc
    31741 aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc
    31801 atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg
    31861 ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc
    31921 gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag
    31981 ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat
    32041 tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg
    32101 aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc
    32161 catacttccc atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat
    32221 ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat
    32281 tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc
    32341 ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc
    32401 gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt
    32461 gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct
    32521 ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc
    32581 tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct
    32641 gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt
    32701 gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa
    32761 acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt
    32821 atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa
    32881 gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg
    32941 gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc
    33001 ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg
    33061 atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat
    33121 cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc
    33181 gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac
    33241 tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa
    33301 tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata
    33361 catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg
    33421 gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt
    33481 cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa
    33541 tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt
    33601 gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca
    33661 ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat
    33721 tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc
    33781 aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc
    33841 ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat
    33901 gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct
    33961 atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc
    34021 ctttgcctat tccggctgaa gttttcactt tcaatatctc aacttcaagg tataaggtat
    34081 ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct
    34141 ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa
    34201 caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat
    34261 tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat
    34321 caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg
    34381 ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt
    34441 cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta
    34501 tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat
    34561 cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga
    34621 ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg
    34681 gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc aacgtttcgt
    34741 ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt
    34801 gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc
    34861 tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg
    34921 tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt
    34981 tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat
    35041 aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa
    35101 taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa
    35161 tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc
    35221 attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc
    35281 tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc
    35341 accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga
    35401 ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa
    35461 cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc
    35521 agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata
    35581 gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt
    35641 tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat
    35701 gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag
    35761 acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc
    35821 aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc
    35881 agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata
    35941 tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc
    36001 aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc
    36061 tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg
    36121 tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc
    36181 ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc
    36241 gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc
    36301 cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc
    36361 agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt
    36421 tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga tggccccttc
    36481 tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt
    36541 gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt
    36601 gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat
    36661 catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc
    36721 gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat
    36781 atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc
    36841 tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag tattgctatt
    36901 gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt
    36961 ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt
    37021 gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt
    37081 agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa
    37141 gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct
    37201 aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt
    37261 tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc
    37321 tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt
    37381 tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg
    37441 gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat
    37501 agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct
    37561 gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt tagaatagct
    37621 ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata
    37681 tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat
    37741 aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc
    37801 atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag
    37861 acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg
    37921 tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt
    37981 cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc
    38041 taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc
    38101 tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg
    38161 ctttttattt ttataattta acagtctaca actccatgag aatacttccc catgttaaac
    38221 ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag
    38281 aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca
    38341 tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc
    38401 tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga
    38461 tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat
    38521 aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg
    38581 tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg
    38641 gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat
    38701 cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc
    38761 cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac
    38821 tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag
    38881 atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt
    38941 ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct
    39001 gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg
    39061 tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg
    39121 ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt
    39181 cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta
    39241 agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt
    39301 ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat
    39361 agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt
    39421 ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca
    39481 tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca
    39541 aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct ggttccatgt
    39601 tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac
    39661 ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt
    39721 ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat
    39781 ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta
    39841 aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat
    39901 cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa
    39961 gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc
    40021 agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata
    40081 acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa
    40141 ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag taattataat
    40201 tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac
    40261 gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat
    40321 caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta
    40381 caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat
    40441 aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc
    40501 gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc
    40561 caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca
    40621 gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt
    40681 acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg
    40741 aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa
    40801 aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa
    40861 ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct
    40921 aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg
    40981 tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt
    41041 actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct
    41101 gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga
    41161 ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt
    41221 ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca
    41281 aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata
    41341 tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa
    41401 tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt
    41461 taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt
    41521 ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat
    41581 tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc
    41641 agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc
    41701 ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc
    41761 accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat
    41821 tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc ctgagatatc
    41881 tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac
    41941 cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc
    42001 tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg
    42061 tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc
    42121 aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc
    42181 tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt
    42241 ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat
    42301 aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat
    42361 cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa
    42421 taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga
    42481 acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt
    42541 ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt
    42601 tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta
    42661 actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa
    42721 ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga
    42781 tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac
    42841 ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt
    42901 caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg
    42961 tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag
    43021 acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt
    43081 cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct
    43141 tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt
    43201 tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa
    43261 taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg
    43321 tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga
    43381 tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca
    43441 tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact
    43501 ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt
    43561 cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc
    43621 tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct
    43681 tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa
    43741 gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca
    43801 cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca
    43861 ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga
    43921 ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt
    43981 cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg
    44041 aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc
    44101 ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa
    44161 taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt
    44221 acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc ctctgtagaa
    44281 tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat attatgacta
    44341 ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa
    44401 gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat
    44461 tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca
    44521 cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta
    44581 ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac
    44641 aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac
    44701 ttcttccatc gtatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt
    44761 gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc
    44821 atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca
    44881 catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa
    44941 aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc
    45001 cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa
    45061 cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc
    45121 agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc
    45181 tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac
    45241 agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa
    45301 actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata
    45361 tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat
    45421 accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga
    45481 atccatatac aggaattcag tacggtacag accaactgct tctccaccat tattatggac
    45541 accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc
    45601 tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc
    45661 acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc
    45721 gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc
    45781 aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc
    45841 aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt
    45901 caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac
    45961 gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg
    46021 catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt
    46081 tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat
    46141 ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa
    46201 gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc
    46261 atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc
    46321 cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt
    46381 tagcatttca accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc
    46441 attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca
    46501 cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt atactctaag
    46561 tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg
    46621 atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa
    46681 gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat
    46741 gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc
    46801 aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt
    46861 tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat
    46921 tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt
    46981 ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc
    47041 ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat
    47101 atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc
    47161 aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat
    47221 tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg
    47281 gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc
    47341 caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac
    47401 aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga
    47461 atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac
    47521 gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt
    47581 ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat
    47641 ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta
    47701 gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag
    47761 atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat agtcggtgct
    47821 ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga
    47881 ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac
    47941 gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg
    48001 atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact
    48061 gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg
    48121 ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt gacgatccaa
    48181 atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta
    48241 gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat caataaactt
    48301 caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac cgaaaaagat
    48361 atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa agaaaaagaa
    48421 cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg acaagataac
    48481 gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa caaacagaat
    48541 cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac agagcttgcg
    48601 aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt cgatatgtct
    48661 gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga
    48721 tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag tcttgtcttg
    48781 ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca aatcttagat
    48841 gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac gatcatcatt
    48901 atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc
    48961 gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc agaatttctg
    49021 aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt gatgacaatc
    49081 gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact tcacatcgaa
    49141 gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc tatgggtgct
    49201 cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga attttatctt
    49261 gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa aatcatcgtt
    49321 acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga ataaatccag
    49381 ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt ttcagaaaag
    49441 tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag caattaaaaa
    49501 tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca ttcccgtctt
    49561 gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac agccggaaat
    49621 accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat caatgacaaa
    49681 cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga taaaatcaaa
    49741 gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc
    49801 tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt tgaacataat
    49861 aattaaagag cgtgggacaa aagtaaa
//
LOCUS       DAFEZB010000014        47853 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFEZB010000014
VERSION     DAFEZB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.014"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(38..1189)
                     /locus_tag="LP859_000845"
     CDS             complement(38..1189)
                     /locus_tag="LP859_000845"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL3028818.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="LP859_000846"
     CDS             complement(1202..1639)
                     /locus_tag="LP859_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3028819.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="LP859_000847"
     CDS             complement(1654..3420)
                     /locus_tag="LP859_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL3028820.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="LP859_000848"
     CDS             3684..4361
                     /locus_tag="LP859_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028821.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="LP859_000849"
     CDS             4364..5491
                     /locus_tag="LP859_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028822.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="LP859_000850"
     CDS             complement(5691..6131)
                     /locus_tag="LP859_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3028823.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="LP859_000851"
     CDS             complement(6142..8175)
                     /locus_tag="LP859_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3028824.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="LP859_000852"
     CDS             complement(8177..9880)
                     /locus_tag="LP859_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3028825.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="LP859_000853"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="LP859_000853"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL3028826.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="LP859_000854"
     CDS             complement(10963..11988)
                     /locus_tag="LP859_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL3028827.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="LP859_000855"
     CDS             complement(12197..13066)
                     /locus_tag="LP859_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL3028828.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="LP859_000856"
     CDS             complement(13525..15417)
                     /locus_tag="LP859_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028829.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="LP859_000857"
     CDS             complement(15414..17141)
                     /locus_tag="LP859_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028830.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="LP859_000858"
     CDS             complement(17157..17807)
                     /locus_tag="LP859_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL3028831.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="LP859_000859"
                     /pseudo
     CDS             18011..18307
                     /locus_tag="LP859_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18479..19099
                     /locus_tag="LP859_000860"
     CDS             18479..19099
                     /locus_tag="LP859_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL3028832.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="LP859_000861"
     CDS             complement(19191..20357)
                     /locus_tag="LP859_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3028833.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="LP859_000862"
     CDS             complement(20369..20722)
                     /locus_tag="LP859_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL3028834.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="LP859_000863"
     CDS             20850..21872
                     /locus_tag="LP859_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL3028835.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="LP859_000864"
     CDS             21891..22052
                     /locus_tag="LP859_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028836.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="LP859_000865"
     CDS             complement(22311..22745)
                     /locus_tag="LP859_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028837.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="LP859_000866"
     CDS             complement(22855..24714)
                     /locus_tag="LP859_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL3028838.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="LP859_000867"
     CDS             complement(24832..25992)
                     /locus_tag="LP859_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBL3028839.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="LP859_000868"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="LP859_000868"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL3028840.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="LP859_000869"
     CDS             complement(27739..28437)
                     /locus_tag="LP859_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL3028841.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="LP859_000870"
     CDS             complement(28394..30055)
                     /locus_tag="LP859_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL3028842.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="LP859_000871"
     CDS             30273..31673
                     /locus_tag="LP859_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL3028843.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="LP859_000872"
     CDS             complement(31925..33004)
                     /locus_tag="LP859_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3028844.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="LP859_000873"
     CDS             complement(33090..34049)
                     /locus_tag="LP859_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL3028845.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="LP859_000874"
     CDS             complement(34061..35572)
                     /locus_tag="LP859_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL3028846.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="LP859_000875"
     CDS             complement(35594..36157)
                     /locus_tag="LP859_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL3028847.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="LP859_000876"
     CDS             complement(36163..37026)
                     /locus_tag="LP859_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3028848.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="LP859_000877"
     CDS             complement(37040..37936)
                     /locus_tag="LP859_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028849.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="LP859_000878"
     CDS             complement(38111..39400)
                     /locus_tag="LP859_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3028850.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="LP859_000879"
     CDS             complement(39372..40889)
                     /locus_tag="LP859_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL3028851.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="LP859_000880"
     CDS             41087..41977
                     /locus_tag="LP859_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL3028852.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="LP859_000881"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="LP859_000881"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL3028853.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="LP859_000882"
     CDS             complement(42886..44358)
                     /locus_tag="LP859_000882"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL3028854.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="LP859_000883"
     CDS             complement(44995..45357)
                     /locus_tag="LP859_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL3028855.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="LP859_000884"
     CDS             45580..46332
                     /locus_tag="LP859_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3028856.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="LP859_000885"
     CDS             complement(46472..47395)
                     /locus_tag="LP859_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3028857.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="LP859_000886"
     CDS             complement(47410..47682)
                     /locus_tag="LP859_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028858.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13931 a         9944 c         8258 g        15720 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttaatat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacattt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttatgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aat
//
LOCUS       DAFEZB010000015        46961 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFEZB010000015
VERSION     DAFEZB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.015"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(253..2019)
                     /locus_tag="LP859_000887"
     CDS             complement(253..2019)
                     /locus_tag="LP859_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028859.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVTMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1979..3733)
                     /locus_tag="LP859_000888"
     CDS             complement(1979..3733)
                     /locus_tag="LP859_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028860.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3843..4412)
                     /locus_tag="LP859_000889"
     CDS             complement(3843..4412)
                     /locus_tag="LP859_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL3028861.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4430..5845)
                     /locus_tag="LP859_000890"
     CDS             complement(4430..5845)
                     /locus_tag="LP859_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3028862.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5856..6524)
                     /locus_tag="LP859_000891"
     CDS             complement(5856..6524)
                     /locus_tag="LP859_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL3028863.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6655..7239)
                     /locus_tag="LP859_000892"
     CDS             complement(6655..7239)
                     /locus_tag="LP859_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3028864.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7570..7803)
                     /locus_tag="LP859_000893"
     CDS             complement(7570..7803)
                     /locus_tag="LP859_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL3028865.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7818..8766)
                     /locus_tag="LP859_000894"
                     /pseudo
     CDS             complement(7818..8766)
                     /locus_tag="LP859_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(8766..9608)
                     /locus_tag="LP859_000895"
     CDS             complement(8766..9608)
                     /locus_tag="LP859_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL3028866.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            9930..11117
                     /locus_tag="LP859_000896"
     CDS             9930..11117
                     /locus_tag="LP859_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL3028867.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(11209..11949)
                     /locus_tag="LP859_000897"
     CDS             complement(11209..11949)
                     /locus_tag="LP859_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL3028868.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            12637..12795
                     /locus_tag="LP859_000898"
     CDS             12637..12795
                     /locus_tag="LP859_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028869.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            12867..14087
                     /locus_tag="LP859_000899"
     CDS             12867..14087
                     /locus_tag="LP859_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3028870.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
     gene            complement(14180..14572)
                     /gene="rpsI"
                     /locus_tag="LP859_000900"
     CDS             complement(14180..14572)
                     /gene="rpsI"
                     /locus_tag="LP859_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL3028871.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(14588..15031)
                     /gene="rplM"
                     /locus_tag="LP859_000901"
     CDS             complement(14588..15031)
                     /gene="rplM"
                     /locus_tag="LP859_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL3028872.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(15550..16017)
                     /locus_tag="LP859_000902"
     CDS             complement(15550..16017)
                     /locus_tag="LP859_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL3028873.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(16137..17675)
                     /locus_tag="LP859_000903"
     CDS             complement(16137..17675)
                     /locus_tag="LP859_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL3028874.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            17755..18441
                     /locus_tag="LP859_000904"
     CDS             17755..18441
                     /locus_tag="LP859_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL3028875.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(18642..19169)
                     /gene="msrA"
                     /locus_tag="LP859_000905"
     CDS             complement(18642..19169)
                     /gene="msrA"
                     /locus_tag="LP859_000905"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL3028876.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(19253..19930)
                     /gene="pgmB"
                     /locus_tag="LP859_000906"
     CDS             complement(19253..19930)
                     /gene="pgmB"
                     /locus_tag="LP859_000906"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL3028877.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(19927..21057)
                     /locus_tag="LP859_000907"
     CDS             complement(19927..21057)
                     /locus_tag="LP859_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL3028878.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(21057..23240)
                     /locus_tag="LP859_000908"
     CDS             complement(21057..23240)
                     /locus_tag="LP859_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL3028879.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIAPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(23237..24262)
                     /locus_tag="LP859_000909"
     CDS             complement(23237..24262)
                     /locus_tag="LP859_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3028880.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(24307..25134)
                     /locus_tag="LP859_000910"
     CDS             complement(24307..25134)
                     /locus_tag="LP859_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL3028881.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(25131..26198)
                     /locus_tag="LP859_000911"
     CDS             complement(25131..26198)
                     /locus_tag="LP859_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL3028882.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(26224..27060)
                     /locus_tag="LP859_000912"
     CDS             complement(26224..27060)
                     /locus_tag="LP859_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3028883.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(27057..27926)
                     /locus_tag="LP859_000913"
     CDS             complement(27057..27926)
                     /locus_tag="LP859_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3028884.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(27996..29279)
                     /locus_tag="LP859_000914"
     CDS             complement(27996..29279)
                     /locus_tag="LP859_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL3028885.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(29293..30978)
                     /locus_tag="LP859_000915"
     CDS             complement(29293..30978)
                     /locus_tag="LP859_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL3028886.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            31253..32278
                     /locus_tag="LP859_000916"
     CDS             31253..32278
                     /locus_tag="LP859_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3028887.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(32873..36547)
                     /gene="rpoC"
                     /locus_tag="LP859_000917"
     CDS             complement(32873..36547)
                     /gene="rpoC"
                     /locus_tag="LP859_000917"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL3028888.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(36605..40222)
                     /gene="rpoB"
                     /locus_tag="LP859_000918"
     CDS             complement(36605..40222)
                     /gene="rpoB"
                     /locus_tag="LP859_000918"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL3028889.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            40950..41918
                     /locus_tag="LP859_000919"
     CDS             40950..41918
                     /locus_tag="LP859_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3028890.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            41936..42841
                     /locus_tag="LP859_000920"
     CDS             41936..42841
                     /locus_tag="LP859_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028891.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            42838..43644
                     /locus_tag="LP859_000921"
     CDS             42838..43644
                     /locus_tag="LP859_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028892.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            43780..44769
                     /locus_tag="LP859_000922"
     CDS             43780..44769
                     /locus_tag="LP859_000922"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL3028893.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(45086..45610)
                     /locus_tag="LP859_000923"
     CDS             complement(45086..45610)
                     /locus_tag="LP859_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL3028894.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            45897..>46961
                     /gene="scm"
                     /locus_tag="LP859_000924"
     CDS             45897..>46961
                     /gene="scm"
                     /locus_tag="LP859_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL3028895.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        14006 a         9601 c         8192 g        15162 t
ORIGIN      
        1 ctctacttaa aacggcaata tgaaagaaac tataactttt tattcaatct tttatgttta
       61 actggtttat cgccaatttt ttaaaacttt gattattcct tatattataa gaatagacat
      121 taagtgaact aattcaatca tactgattct ttcgtgtatt caaaattaga tgctgtttaa
      181 taaaattatt ctttttgaaa aagaattata actatatccc aaaaattgta ggttaattta
      241 aagcctattt catcatcgtg attgagcttc tttttcatag acgaatttct cagatagttt
      301 agttttcttt atgagatttt tgtatatttc tgaccttcga tacaactcat tatgagtacc
      361 ttgatcaact agttttcctt gatccatcac tacaatttta tttacctttt caatagactt
      421 caagcgatgc gatataatta gtactgtctt atcttgtatt aaacgattta aactttcctg
      481 aatacgtttt tcattttccg catctaaact ggcagtaatt tcatctaaaa ttaaaattgg
      541 cgcatttttc aaaaaagccc gtgctatgga taggcgttgt cgctctcctc cagaaagtga
      601 acttccgttt tctccgacca atgtatgata accgtcaggt agtttttcaa taaagtcttc
      661 gcagttagct aaacgagctg cttttttcac ttcctcatct gacgcttgac tatttccaat
      721 acgtatattt tccaaaatac ttgtattaaa caaagttacc tcttgaaaaa caatagcgat
      781 tttgctaaac aaagaagctg gagatacacg tttaatatca tacccatcaa ttaaaataca
      841 gccttcatca taatcataca gtctagacac taatttcaag atactagttt tcccggaacc
      901 actagcacct actaacgcag tcacttctcc ttgtttagcc gtaaaagaga tatgatctaa
      961 aattggggta ttgttatcat atgagaaaga gacatctcgc aactcaatgt caaaagattt
     1021 cagaggacta tctgatccct cttggatact ggtttccttc atagctctaa ttcgctgtat
     1081 tttaggagct aaataaaata tttcaagcac tgcttctttc atagaatcga aggaatcctt
     1141 tatttttatt gcggctagta agtaaccaac gacgtagagt atagtcactt cacccgttaa
     1201 cagtaagtga acacctacta gaatgactac tgctaaacta acatatgaga aaatagagga
     1261 taaggccatg actgaaaaag tacttaattc tacctttaaa tgaatccttt cactctcttc
     1321 catttttttg aaaagtctgt cttgaacttt tttagataga ttaaagctat taatctcttg
     1381 ctgcaattca atagtttctt gaaattcttc cgagttttct ctcaaagtat cgtaatattt
     1441 agtattggct ttcgtttggg atttctttga taacaagatt aagacaaaac taaataacgt
     1501 tggcaaaata acagctaatc ccattgtgac attaccaacc aaaagcatca ctgaaataaa
     1561 agggaaaaac agagccatac cgccggcttt aggtattgca tgactcatcg catgctcaat
     1621 accttcaacg tcagacatga tagtttgaga taaatctgat aaattatgtt ttgaaaaata
     1681 ggataatggt aaatttgaca aatcgtctgc aatttgcact cttaaatgcg cactttcttt
     1741 ataagtacta ttatataagt tttcatactc cctatctaac aaccaataca tgaaaagcaa
     1801 agtaacaaac gatactgcaa aataaagcca acggggcttt gcattttcta aaactaattc
     1861 gtctatcagc atcattataa tgaatgctgg taccatatta atgcagtata caaaaaaact
     1921 agaaatagaa gctttagtta aagcttctgc gccctgatca gttaaagcaa attttttttt
     1981 ataaaaattt ttcattgtca agcctccaat catttgcatt ctcataatta gtgaccaatt
     2041 gtttatacac tcgatgttta gccattaatt cattatgatt tccacgttct actatttttc
     2101 cttctgacaa gacaagaatt tcatctacag atttaatgct ggataatcga tgagctatca
     2161 taataactgt tttttctttc atcaaatatt gaaaagcctt ttgtagttca tactcattat
     2221 ctggatcaat agatgcactg gcttcatcca taataacaat ctttgcatct tttaaaattg
     2281 ctctggcaat agctatacgt tgtttttctc ctcctgacag ataaactcct ttactaccaa
     2341 ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg gctaatctta
     2401 gcgcgtcaaa tactttactt gctggcgctt gaggatttgc tatagaaacg ttatcgtaaa
     2461 tactctgatt gaatagtttc gtatcttgaa agacaaaagc aattgtgtta ataattgtgt
     2521 cttttgaata gtgatttaaa ggcttaccac caattttaat ctgtccctta tccacattgt
     2581 agaaacctga aattagttta gctatcgtac tctttccgct tcctgatttt cctactaatg
     2641 cataaattct tttttgttct aaaactaagt tgaaattttc taaaacacac tcttctttgt
     2701 atgaaaagct gacattttca aatgcaatgt cacaattttt aatctgtgtc tcgttcccat
     2761 attttagttg gtcttgcttc atttctaaat aaagggagtc tagtgtttcg actgcatatc
     2821 ttgcttcaaa caagtgcata cttacataca ttattctcat gaaagaaaca aaaataattc
     2881 cacttaaaaa aaacaacatc aacaagtcaa cagctaatat cttgggagtg gtaagagaag
     2941 ttataaaaaa ggttatagga actatcaaaa tgcaaactat gccaaaaaat agccattgaa
     3001 aaagaacaaa aggtgttttc ccactcaaag aatacttata tgcaaactta gcataatcgt
     3061 taattgcttt attcaaggct ttaaatgatg ttactttagc accaaatatc tttataactt
     3121 gaattccgcg gatatattct accgtttcag agcttaacac atctaacgat tcttgatatt
     3181 tttgaataaa atttgtattc tctgtcattt tctttaacaa gaatacacca attactaaca
     3241 tggcgattag aaccaatcca attttaaagt taatgaaaaa gcctaaggca actgataaaa
     3301 caggtgttac tatggcttga gcactatctg gaatcaagtg agcaattgcc atatgtgttt
     3361 tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga agaatttaaa tcaaaaaaac
     3421 gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc tctaagcgaa
     3481 acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct aaagttaacc
     3541 aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca ataattagag
     3601 actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca gaaaaatcag
     3661 aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta aataattttt
     3721 tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg tttacaactg
     3781 ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagatagaag gtaaactacg
     3841 ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat catagataaa
     3901 cttgctacaa ctaacactga aataattccc catataccca ccgcgtagtg gatcattgca
     3961 tccattgttg cagcagattg tcctctatct atccagtctt gacgaaaacg atctgtaaag
     4021 aaccatatgg gaatccactg tccggcagca ctgcctagtt gcattagaac ccaagacaca
     4081 acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc taagatcgcc
     4141 atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt aaaaaataac
     4201 aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata gctaatataa
     4261 acaaatatga ttccagataa taatccaaaa atccctgggg atatgctgtg accaaacgcc
     4321 ccaccaatac tactaaccat tattgaaaaa aagtaaatta ctagggaaat aactgtagct
     4381 aaaccaataa aaatataatc ttttgttttc atatatattc tcctcaattc tatttcgtta
     4441 tgtcattaaa aatctgttct atcaaagttg aatcttctac tctaagtttt atatcttttt
     4501 ttattccacc attttttaaa taaataattc tattgcatgt tttataaaga aattccggat
     4561 catgagtggc tataactact gcaccctttt cttttaagtc tttaataagg tcagcaatcc
     4621 tttccatatt gatataatcc aacccacttg taggttcatc aaatagaaat aaattagttt
     4681 ttgacaatga agctatagaa accaataatc tttgtttttc tccaccggat aaatcaaacg
     4741 gtaattgatc tttcatttta taaagttcca ttttttttaa aattttttct tgctgatctt
     4801 tactaactat ttcattcccc agggctaact cgctcctcac tgattctgtg aaaaattggt
     4861 aatccatttc ttgcattact aaaaacggcg atgacaaatt ttttttattt tcgattatct
     4921 ttcctttggt agggcgaata atcttcatga gtattcttag taaggttgtt ttccccacac
     4981 cgtttttccc taccaaacca ataacatctc caactttgac atcaaaattt atgtctttta
     5041 gaatattttc ataagtaatt tcttcaactt ttaatacatc tgcacttacc ttttcgatat
     5101 agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta ttgttttcag
     5161 tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat agaacttgat
     5221 caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta aatcctttat
     5281 tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc aaatttgaag
     5341 acggttcatc caatataatt aatttagagt ttagcgataa actacatcca atagaaattt
     5401 tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta
     5461 ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt
     5521 ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt
     5581 gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat
     5641 tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata agtccattca
     5701 ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactgc
     5761 ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa
     5821 aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa
     5881 atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa
     5941 cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca
     6001 atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga
     6061 aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa
     6121 cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa
     6181 tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac caacaaccat
     6241 gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtccc aaaaaatata
     6301 gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa
     6361 atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca
     6421 ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa
     6481 aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa
     6541 aataagatcg cactgttatc ttaagctgac aaaaatgcac cgttattcag ataacagtgc
     6601 gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt ttaattaact
     6661 cagtaattca atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt
     6721 cttttctttt gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat
     6781 gtagaagtgg atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt
     6841 taacttatct agacaacaga ctgtcttttt tgtcacaaat gatgtaattt tttcaacaat
     6901 atcttgatat ttggttccag ctgattggaa taaaaccaaa tcgaatatac gtttattctc
     6961 aaaaatataa ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat
     7021 atctatattt tctaagttta agaggtattt ggtaaactct tcattataca tattaataag
     7081 cgcattaata tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt
     7141 ataaaaagca cccgtagtta cgcctgcggt caagcacagt tgccttatct tagtttgctg
     7201 aaatccttct ttttcaaaat ggcttagtcc actttctatt aatttttggt gagttgtaat
     7261 ataatctctg gtttctatgg gtacttcctc ctaataagat gaattaacta tattatctta
     7321 cacaggactt gaagtttcaa tcgttaacgt ctattactga actcattcct tagagctaaa
     7381 ctaatgagaa agagcaaaat aactgataca aaaagcaatc ctacaagaat taatagactc
     7441 atctaaatca ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag
     7501 ctattagggt aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa
     7561 taaagtcttt tacttaaaac cttttttgta atcgtcaata aattttttcc ctttatcagt
     7621 taattgaaat cctatatcta aatagtcctc atatctaagt ttagccagca tctcacttac
     7681 agatgctgga ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct
     7741 ttttgataat tcctgaattg ctagtaaata aattagtata gtagtcgttt taatttgtac
     7801 catacctacc ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc
     7861 atcatcgcat agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc
     7921 gttgaataga taggaatatt ggtatctttt gatactgttt tcattggtct atcgtccaca
     7981 ctgctttcta cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt
     8041 atttgatctg gtgttccttc tttcttcagt gttgatttcc caaatgtaag cagaaggcac
     8101 attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca tttttttatc
     8161 attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca atttttcaat
     8221 atacttatct agattttctt tatagaattt tttattatca ggatcttttt ccgctaactg
     8281 tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag catggggatc
     8341 ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat cacttgctgc
     8401 aaaatagtct ttgttttcct ctttgttcgc attttttact aattttgtaa accaagcatt
     8461 tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg aagttttttg
     8521 aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta cgatactgtg
     8581 taaatcaatt ttatcttttg ctatattttc agtaatatct gctaaaatcg aattagtaac
     8641 tactaccttt aatttttcgt tcgaagccac tgtatctttg tctttactat ttgttgaaca
     8701 tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg ctttccattt
     8761 tctcatcata actttctctc cttctttctt aaaaattttt gttttggtga gattagaaaa
     8821 ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc tatgttaaaa
     8881 ctataaccaa tgaacaaacc tagcaatgat gagcacgctc cgactgtcgc cgaaataaat
     8941 atcatagttt ttaagctgtt tgcatataaa tatgctgtag cagctggtgt aattaacatt
     9001 gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa agttaataga
     9061 atcattaata gataatgata aaaactgaca ttcatcccca ttgcttttgc cattaatgga
     9121 tcaaaagaag tgattaataa ttctttaaaa aatagaacaa ctgtaaccaa cactaaaaca
     9181 gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt tccaaataag
     9241 atatgaaata aatccgttga gctatttgca actcctataa ggataactcc taatgctaaa
     9301 aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt gctttttata
     9361 aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa aaaattaata
     9421 cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc atcacccata
     9481 agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat ccctataact
     9541 acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt aataaaattt
     9601 tgaagcatat tattccccct tgtatattag agttagggtt ttaaccttcc ggttttgatt
     9661 ggataaccct atgaaccatc gttattttaa tttggtatta actcatttct tttgagataa
     9721 tatatttatt ggattgagag gctaagccgt tccccccttg ggtcattgaa cccgcactcg
     9781 agagactggc tcctctctct gataagtgta ttgcgtatga gttagatgtt gaaagtcctt
     9841 actttactcc gtatctcgta caaggtagtg acgcttttca gttgagtgga tttcaatcca
     9901 aaattctttt tttgaaaggt cagatataca tgtacttttt aggcattgat atcggtaaac
     9961 gcactcacgt tgcttctatc atgaacgagg aaggaaaggt attactaaaa ggtttttcat
    10021 ttcctaacac cactgaagga gcagagtcat taattgagcg aatggtcgac tattccggag
    10081 cacccagtga ctttgttata gggatggaag ccacggggca ctattggctg tccatctttt
    10141 cataccttca cgaatcagat tatttgattc acgttgtgaa tccccttcaa acagacggat
    10201 ggcgtaaagg tacagaaata cggaaacgaa aaaacgatat tattgattct gttttgattg
    10261 ctgacttaat gcgctatggc tcatttgtcg aaacggtctt atccgatgaa aatgtttttt
    10321 ctttgaaaca actttctcgt taccgaactt atttagtagg tacagcgagc gacttcaaaa
    10381 gaaaaattat agccgtattg gatcaagtct ttccagaata cgcgacgatt tttaccaaac
    10441 agggcgtttt cggaaaagct tctaaagagt tattgctggc gtttagtgcc ccgatcgact
    10501 tagaaaacat ttcctcagaa actttggccc aatacttagc tgagttaagc aggaaccgaa
    10561 tcaaggcaga aaaagcggac caattgaagc aagcagcttc taactccttt ggagtaaaat
    10621 ttgctcaaga agcgttcacc ttccaactac gttcaatgat tgaacaatta aaattcattg
    10681 aaagccaaat aaaggaaacg gaaagagaaa tcaagcacat aatggacact ttaaattctg
    10741 ttattctcac aattcctgga attggtccta taaacggtgc gactattctt ggtgagatcg
    10801 gagatattca aaaattttct aacccgaaaa aactcgttgc ttacgctggg atagacgctt
    10861 ccgtgacaca atcagggcaa tatgaggcaa cgcataatgt tatgagtaaa cgagggtctc
    10921 catatttgcg aaaagccttg ttttcggcgg ctttagtagc ttctcaatgt gatcctgttt
    10981 ttagtgcgtt ttaccagaaa aaagtttccg aaggaaaaca tcatctaact gccttaggtg
    11041 cggtttctcg taagctttgt tacgtgattc atgctatttt gactaaaaac gagccttata
    11101 taattgtttc tgattagcta ttgaacattc aaaaaaacta atggtaagaa gcttatttgt
    11161 tgtacccttt tttcggaatt atctgttata aaatattttt tgatttggct aatttttact
    11221 tgactctata tagttagtct ctccaataaa tatagtatct ccataggctt tttttaaatt
    11281 ctcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat tcaaaagaat
    11341 aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta gcactgtttt
    11401 tccctgatgt ttcaattctt ttaacgtatc cataattatt cgttcactaa ctaagtctat
    11461 ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac atcttgctaa
    11521 aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag aatattttcc
    11581 catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt taattctttg
    11641 aaaaactttc attccggcat atgttcccat agatacacat tcttttactt ttattggaaa
    11701 agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt ttttattgat
    11761 atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca ttgctttaag
    11821 caatgtagat tttccagcac catttggacc aataatacct gttaaagcac cttcttgtat
    11881 ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa gctttttcac
    11941 ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat ggtatacaaa
    12001 aattatattt tagtcaaccc taaattttat aatttagatt tttttctgtt gctgaagatt
    12061 agaatgacaa aactatttga aactacgcgt atagtaagta gctttcaaaa tatataaaaa
    12121 aggagcgttc tcaaatgaga acatgctcct aaaactactt tattggcgta tggaaaaatt
    12181 atacttaaag tggatagcgg gttgaataag ttgataagca tcaaaacatt tataaaaatt
    12241 attcaagact atgtctgttc catatacaaa tatgaaaatc agtaaactcc atttaacttt
    12301 tttcgtagtg tgtcttttta tacatgttta tcgttattaa tagtgaatag gaaaaaacct
    12361 atttttgatc attgacaatt aaatacaatt tttgaataac gatccctgct attcgagcta
    12421 gaaactcaag tacgccatga cctgattagc gacaaatact tagagatttc tttttgttct
    12481 gggaaaacaa aatgccatga caatagcagc aaataatgat acttccgtaa ggctcgccgt
    12541 aaactgggga gaggtgaaat tcctatgtgc ttacccaaag caattcaaaa attctaatct
    12601 tttttaggag atgattttat aaaattgaga gtctacttga ataaaaaaca aacctgtgag
    12661 tttctaggaa ttgataataa tactttagat aagtggattc gtatatataa ttttccagta
    12721 atcagaattg acggtatagt tcgctttgac atagaggata tcaaagattt tatgaataca
    12781 tttaaaacaa tttgaaaatt tcttctcgcc gcatatacta agaatagcga aatatactgc
    12841 cttacttttt aagggagtga ggaattttgc ctacaaaact ttcaaatggg aaatataaaa
    12901 ctaatctgag gtatccaaaa aaattcagag aaatcactgg aattacatca gaaaagtatc
    12961 aaaaagtatt tccaactagg caattggcta ttaaagccga aaatgcaatg aaaaggaaaa
    13021 ttgaaacagt tttaagagag gagaatgcaa attcactaga attaaaagga aaaataaaat
    13081 ttaaggaatt ctatgaatca aaatggcttc ctagatatga actaggtcag actactagat
    13141 caaaaagagc tccaagttac gtaacaatta gtaatactaa agatatattc cgattgcaca
    13201 tccttccaat gtttggtgag tatgcaatga actatctaaa ttcaaatact gagataatat
    13261 cagatgaatt aacaaaaaaa tcgaaagaat acgcaaatat aaaaattatc aaaggatatg
    13321 tccgttctat gtttgatatt gcagaaatac taaattatat tgaatttaac cgaactacca
    13381 aaattattca atgtatcact gcacctaaaa aaattgcttt agaagaaaga aggatacggg
    13441 aaggtaatca agcattatcg tcagaagagc ttattgcttg gatagaagca gtaaacactg
    13501 acttgaataa tcacttgctt actcttcatg actatactct tttcatgctt accctatatc
    13561 ttggtgatag aaaaagtgag acgtatgctt tgcaatggaa atatatcgac tttgagaaac
    13621 aaacggttcg ccttaaacat gctttggata aatatcaaaa aaagaaattt acaaaaggtc
    13681 gaaaggatac cgtaattcaa gtacccgaaa ttgtaatggc attactaagc aaatggaaat
    13741 cagtgcaagc tgagcaattg ttgagattaa aaattaatca aacaccagat cagtatctct
    13801 ttacctatac taaaccatcc ggagaagtaa attgccctgt tcacgctgat tacctaaact
    13861 atcgaatcaa ttcaattaaa agacgacatc ctgaattggc tcatttatct ccacataagc
    13921 ttcgtcatac ctatgcaact atagctagac aaggcggagc aaatatgaat caaatatcta
    13981 atgctttaac acactcagat atttctacta caaagatata cgtaaacaca ccagatgtag
    14041 ttgataagac tgttttcgaa gcatttcaaa aaggattaaa gaactaggat gaatgtgttc
    14101 tcgaatgtgt tcttaaacta aaaaaagaca ctttccaaat aattgaaaag tgtcttgaaa
    14161 cattgatata caaagaatat taacgttttg aaaactgtga agctttacga gcttttttaa
    14221 gacctggttt tttacgttcg accatacgtg cgtcacgagt aagtaagcca gcacgtttaa
    14281 gagctaaacg gaagtcagga tcaacttcta gcaatgcacg agcgatacca tgacggatag
    14341 ctcctgattg tccagcgtat cctccgccgt ttacgttaac aaatacgtca tatgcgcctt
    14401 ttgtttcagt aacagcgaaa ggttgattga ttacttcacg caagtcagca tgtggaatgt
    14461 attcttcaac atctttttta tttacagtaa ttttgccagt tcctggtact agacgtacgc
    14521 gagcaactgc gtttttacga cggcctgtgc cgatatattg aacttgtgcc aatgaaattc
    14581 cctcctatta gattaagttt gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat
    14641 gttctgctcc accgtatacg ttcaattttg taaattgttt gcggcctaaa gtgtttttag
    14701 gaagcatacc ttttacagaa gtttcgatca aacgacgaga atttttagca cgcaattcgc
    14761 cagcagtgat tgatttcaat cctcctgggt agttgctgtg acggtaataa attttgtcag
    14821 tcgctttttt acctgttaat ttcactttgt ctgcattgat gacgatgacg aaatcgccag
    14881 tatccaaatg aggtgtgaaa gttggtttat ttttaccacg tagtacagat gcaacaactg
    14941 ttgaaagacg tcccaatgga acatcagttg cgtctactac ataccattta cgttctactt
    15001 cgcctggttt ggccatatat gttgtacgca cgttattttc ctccaattta agttctgatg
    15061 tttgacatac acttcgagtt tccggggctc tttgtggggc aaacaatacc atttaacata
    15121 ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa
    15181 ctttacttct tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa
    15241 aagaggctgt gacaaaagtc ttgtcacaac cccttatttt gaataaacgg tgttctcctg
    15301 ccaactcctc ggtcacaagt cacaatatcc ataaaacata agaagctgtt ttttaaaatt
    15361 gtttcttgtg actcggagct gaacggcagg cttacaacct cttggtaaac ggtgttcaat
    15421 cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt
    15481 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttttcgg tcaagttcaa
    15541 caacagtttt tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga tcatccagat
    15601 acgtttttct gtggctgttt tgaattcgat aaagatatct tgtgttggat cgtcgccttc
    15661 ttctccagaa acttcgattc ctttttggta aagatctgct agataacgat agcctgcaac
    15721 aagtgtttcc aaacgttctg gaatagtacg atcccagcgg ccaatttcat cagggatttt
    15781 tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg
    15841 ttcggatact tcatctaact gagcgttcaa atcatccatg aactcatcca tcattgggtg
    15901 caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa caacagacat
    15961 ttggcttaga tctgcaacta attgatttaa tacttctttg gtttgttgga atttcatctt
    16021 tcttgcctcc taacattttc ttacacttaa aataatagta gactttcaat gaaaagtaca
    16081 acaaaatcat ttacctgaga acgattttca gttcgctttt ttatccgtaa agccaatcat
    16141 ttttcagaat cagctaattc ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa
    16201 tgcttttgtg tcgaaacaaa taatggatag attttctgat attttttatg atcagtttct
    16261 tgtactccaa cttgctcttt caattctatc aattcctgtg cttcactgat actttggatc
    16321 tttccgatgc ttctccagcc taaaatcaca gctcctaatg cggatgcttc tgtgacagta
    16381 gggaaaacaa gcgggtaacc cagtacatca gccatcatct gacgaaaaat cggagattga
    16441 gcaaacccgc ctgtagcacg gatctctgtg agctgatttt cctggcaaac tccttttaaa
    16501 atccgataaa ggttgagaca gatcccttct agaatcgcct tcatcatttc tttttctgta
    16561 tgttgccgct gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc
    16621 tcaccaagta aatagggatg aaaaatcaga ccattggcac ccgctggcac aaaagcgata
    16681 gccgaaagca gtgcatcata tggattttct cctttttctt tagcagattg aacgacttcc
    16741 tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac cattgctagt cgctcctccc
    16801 actacccaat gtttctgatc cagaacataa caaaacgtct ccatttctgg atggaactga
    16861 ggctgagtag taataaaacg aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta
    16921 tcaccaaaag cacctaaccc tagattagaa agcggcccgt ctgcccctcc caaaacaaat
    16981 gatatatcat ttggcaaacc tagttctgct gctgtttttg atggtagacc tgttacttga
    17041 taggtggaag gaaagacttg cggcaattgc tctattcgaa tgttcaagta gtcaagcact
    17101 tcttctgccc attggaattc atggatatca aaatatccag ttcctgaagc tgttccgtag
    17161 tcgctgatca gttcgccaaa caatctataa aagatatagt ctttgatacc gacaaaataa
    17221 gcagtttttt tgaataattc aggttggctg ttttgcagcc acttgatttt gttcaatggt
    17281 gtcattggat gaatcggcgt cccagtctgc cgataaaatt ctttgccaag cacggtatat
    17341 ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat tcgagataac
    17401 ggtgttttct cttcatccaa caatatcagg ccatgcatcg cagaggagaa ggagacaaat
    17461 tgaatgattg tgtctgaagg caactgttta attacttctt ttataacggt gcagacacct
    17521 tgaaagatca attcctgatc ttgttctgcc atcccttgtg tttcttggat caaagggtag
    17581 cgaaaataag cggatgctgc ttcttgggct tcatctgtga aagcaactgc tttggtttgt
    17641 gtggttccga tatctaatcc gattgaaacg ttcatttttt cacctcaaat tttatgtatg
    17701 tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa aagggggaga tggcgtgtta
    17761 ctttatttta tcattggttg ctgtatcgga tcatttttat gtttaaccgc ccagcggatt
    17821 ccgttaggtc actctattat ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt
    17881 tcttggtatg aattgattcc gattctctcg atcatcgtcc aacgatttcg ctgccgctat
    17941 tgcaggtgtc ggttgccctt ctactatttg ttggcagaag ccttatgtgg tggtcttttt
    18001 gcctggttct ttactttttc ttccgcaaga aatttttcga cgttcatatg gatgctttcg
    18061 gctttgctat tttcgttgat ggatttattt tacttcatgg tccattctca cacgctattc
    18121 tgttcttggg caattttatg gattttttgg ttgcaggccg gcgtcttcca atggcaaagt
    18181 gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc ctatctcgga
    18241 gctggcgata ctctgttgat gctttcttgg agtggtggct tgtctctaga agagttgctt
    18301 cagatcttgt ttcttgctag tctgtttgga atatccgttt tttctgttta ctttatctgt
    18361 cataaaaaaa agttggaaaa gctgcctttc attccttttt tgagcagtgc gttgctgatt
    18421 gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca acctctgata
    18481 tatagtgttc tcttaccaaa tccttgtgac ttggagctag gcgacaggtt cacaacctct
    18541 ccactaaatg gtgttcaatc agctgaccct cagtattagc tcaaaatgct ggttttacaa
    18601 cctctgatat acggtgttcc ataagtagct ttttcggctt cttatttctt gacgccccaa
    18661 tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat aaagattgta atggagcttg
    18721 tttttcttgt aataatcctg atggtaatct tctgcaggat aaaattcgct tcttggttcg
    18781 atttttgtca cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc
    18841 tctgcaatat ttcgttcttc ttcatcatta tagtagatca ctggacgata agaatcaccg
    18901 cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt gctgccaata gatttccacc
    18961 aactgatcat aagaaataac ttctggatca tattcgatct ctactgcttc tgtatgccct
    19021 gttgtgcctg ttgttacttg ttcataagtt ggatttggta catgcccgcc agtataaccg
    19081 gatgtcacag aaatgatccc tggctgctct tcaaatggtt tgaccataca ccaaaagcat
    19141 ccgcctgcga aaactgcttt ttttgtcata taaaatcccc cttttttctc atcatacctt
    19201 gatttccaaa aggaggataa cgttttgctt gtgacagaaa aaacggaaac tattatatac
    19261 gtgatacggc ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag
    19321 gaatcacata ttcagcagaa ggaagattta tgttttcacc tattcccaat acatgcatgt
    19381 taccagccaa agctgcttga caaccagctt ctgaatcttc aatgaccaag catgcatttg
    19441 gcggtacatt caattgctgt gctcctttga gaaaaacttc tggatcaggt tttgcttttg
    19501 atacttgcgt gccgtcaata aggacatcaa aataacttgt caaattcgtt ttttctaaaa
    19561 tgaggcgtgc atttttgctt gctgatccca atccgatctt gatgtttgtt tttttcaagt
    19621 actcaagaac agctattgca ccaggcaaaa cggatgtttc atccattttt tgaatcgctt
    19681 gaagatatag attgtttttt tgctcagcaa aggctgattt ctctttttct gagaatttct
    19741 gttccatttt tccaaatgac agcaatcgct ctagtgactc ggttctggat attcctttca
    19801 gttgctcgtt ttctttctca gtgaatggaa tggacagctg gttagctaaa accagccacg
    19861 cttcgtaatg ataacgagct gtgtctacaa gaacgccatc taaatcgaat agtgctgctt
    19921 tgattttcat cgattacctc cttgaaaatg aacgacttga agtaaagatg cgatgttttt
    19981 agctgtgcga tgaagattat cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc
    20041 aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc ttcctttacc
    20101 aacacttccg caaatagcta tcacaggaac gccctctggt actcttctag caatcccaac
    20161 tggcgctttt ccttttagac tttgtccatc tagacgtcct tcgcctacga ttacgaggtc
    20221 tgcttgctgt acccgtttat cgaactccag taaatccaat acgtagtcga tgccagagac
    20281 tattttccct gaagcaaatg ctaacagtcc tgcacccatt ccaccaccag cactactgcc
    20341 cgatgcctcg attacttgag gggcaaatct catataaaaa tcatgcatgg cttggtcagc
    20401 cactgctaat tcattttctt tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc
    20461 ttttccgcat aaaagattag taacatcagt aatgacagtg attttggctt taatcagctc
    20521 aataggcacg ttcgttttat caactgtagc aattttacct agattttctc ccagtgcttc
    20581 tactttttga ttatcttgat cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc
    20641 gccatcgttg gtactacttc cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa
    20701 caattgtcgt atcagttctc ctactcctcg cgtggataac ctaagtggct gttgttggtt
    20761 tagtgggaca tctttcaatc cgacgatttc tgccatttca aaaatcgcag tttgaccgtc
    20821 tgaagcgtac tggatttctt tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg
    20881 cttcaacctt aaactgtctg ctaatgatga aaccgttcct tctcctccat cgcctatagg
    20941 tagcagatca tattcagcct gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc
    21001 tgcctctaag gctgtcagac tttctttgaa agaatctggc gcaatcagaa tacgcattat
    21061 tttcccctcc gttccaagat cagttctttt tccagctggt attggtctcc attgacaaaa
    21121 ataggtaatg tttctttcgt ctcttttttt attactattt gtttttttgt caaaacaaat
    21181 agcaacctgt gatttttcca aataaaagga aaagaaattt tttcccatgc tgaaggaagt
    21241 cttggagaaa tagaaagagt tccattcgtt actgtaatac ctgaaaaacc aaatataact
    21301 gctagccata gtgctcctaa tgaagcagca tgaattccgt catctgatga atgcggatct
    21361 ggtcctaaat caatcaggca cgcttcttgg aataaatgat acgcccattc catttcttgg
    21421 atacggcaag cctctatggc gtgaatcgct ttactcaacg atgagtcgtg gattgttttg
    21481 ttttcatagt aatgcaaatt cttagaaacg attggttgag aaaaatactc aggtaataag
    21541 tataacagca tgacaagatc tgcttgcttg ctgacttgga gttcatttac ttcgctacgg
    21601 gcataatcaa gtaaaataga ctggctgcca ggacgttctt tgtaagtggt caaatcaata
    21661 actggcttac tgagatacgt gtcatcttgc ggaatcagct gttctttgtt aggcactggc
    21721 aaatataact tatctaaaaa attttgacat tctttcaaaa aaagcggatc gccagcttga
    21781 tatttaacag ctaaggccac attataatgc gccagatagt tcgtatatgc attattgtcg
    21841 atatattctg tgtattcatc tggagcaatg actctgtgta tctctaaatg atcatcgact
    21901 tttacagctc tgctcagcca aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt
    21961 tctaaaaacg attgatcctc tgttgcttgg acatactctt gaacggcata agcaaggtct
    22021 gcactgatat gatgttctgc ggcagctgaa gcaacttctt gtcttgttcc ggttttgata
    22081 ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa
    22141 agtgcacctt gatagtgata atctcttgct ttttgccgtg cgccttctaa tcgatcataa
    22201 cggtacagca gcaaattctt tgctacttca ggaaactgat ataaaaagaa aggcaggata
    22261 aaaatttccg tatcccagaa aacatgccct ttataacctt cgccagttag tcccttcgcc
    22321 cctacactaa cacttggatc attgcgggga gtcataatct gtaaatggta agcagcgaag
    22381 tccatcgcta actgatcgaa agaagaatcg ccttggatcg ttatccgatg ctgatgccaa
    22441 aaatctgtcc aataagacat cgactgctct aaaatttctg tataactaac gcttttctgg
    22501 gcttgggcaa tacttttctt aaacgggtct gtttgctgaa gagaggtaga gagcacgatg
    22561 atcttctcca gcgtaaagga cgtatttttt ctgatctttt ctgaaaagga agcggtgatc
    22621 cggcgatttt ttgcttggaa tacacctgct ttattgaatt tagctgcaat cccaatatca
    22681 attttacttt ctgttgtctg gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt
    22741 tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct gtccatcgat acccgttttc
    22801 acatctactt ggcagtcttc ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg
    22861 agattatttt gatcagcaac acgacaagta atgacttcca ctttttttcc ttgagcagtt
    22921 tcaatgagcg catagcgttt aagctctccc atttctaaat ctagtgcccg ctcgtatgca
    22981 agaactttca tcgtctcaag agaaaaatgt tcaccgtcga tttttatttc ccatctgatg
    23041 acatctggga gattcactaa ctcggctgat tctgctccta aaggtcggtt atatacacca
    23101 gctgcaaaca ttccccttac ttgattccga tagtcttcct cctggcttcc tctgatacca
    23161 aaaaatccat ttccaatggt catctgactc gcatacttat tgatatactc taaatcaaac
    23221 tgtttttctt ctactttcat aaggtaataa cttctcctgt ttctgccgaa cgatataaat
    23281 tttccaccag attctggatg acatatcctt gccagccatc tgcaatcatc tgagtctggt
    23341 caccgagcgc ccgattcaca aaatgagcca tacttagttc atgtgggtca ttaactggcg
    23401 tggtcttttg taaggaaacc agctttccat ctacatcttc gaatagttcg cctggataca
    23461 gattagcacc tgcctgatcg ccacaaaaag ccacattcag tttgtcttct ctgatattca
    23521 aagcaaacga agtattcagt tggatgatct tgccgttttc taattccagt gtgccaaaaa
    23581 ttgcatcttc tactgtgaat ttgcttggat cccatttccc gaatttccct gaattttttt
    23641 tcgtaccaat tttttggaag ctgtacgccc gaacgctttt tactttagga aatccagtaa
    23701 tatacatcgc tgtatcaagc atgtgaatgc cataatcgat caaaggcccg cctccttgaa
    23761 gttctttgtc gataaacgtt ccccagcctg gaacaccaga ccgacgaagc gcattcgctt
    23821 ctacgtaata aatatccccg agaacatcaa gatgattctt gatcattgtt gcttcgtcag
    23881 aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt tttatcggct gtctctgcca
    23941 tctttcttgc ttgatcagct gtcatagctg gtggtttctc gcacatcact gaagcattag
    24001 cttcgagggc agccaataca tgttcgaagt gaaagcgatt gggtgtacag acactcacga
    24061 tatcgggaga aatctcattc agcatttgtg taactgatgt atacgcatga gaaaatcctg
    24121 tacgtcggca aaagtcttct gctcgctggc tgactggatc aaccacagcg gccaattcta
    24181 agacatctga tcttttttga taataagcag catgtacttt ttctgctact tgtccagcgc
    24241 ctatgattgc tactttcttc atttatttct ctctcctttg cttatttact tattgttatt
    24301 ctttctctat tctttctcta aatcatttaa atgacgcgct gtttccagac agtttttcaa
    24361 gtattgcaat gattcctgat aaattttcac ttcattttct cctcgcaaac gacattcata
    24421 taccatataa ccttcatact cattttgaag tagcgtttga aaatggcgag taaaatcaat
    24481 tgcgccagat cctggttggt agcgatgatt atctgctaaa tggatatgac ccacatactc
    24541 tttttgcgta gtcaatgctt ttgaaatatc atcttcttca atattcatat gataaaaatc
    24601 agcgatgatc tgcacatgtt tcaattgatt agagtcaatg taatgacgcg catccgctaa
    24661 caaattgatc atatggtctt ggtatcggtt caaaggctcc aaataaataa ccgtacctgt
    24721 ttcttcggct actttatcta aataacgaag tgattcgctg actgctttat agtctccttc
    24781 tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg aacattcccc aagctgctgg
    24841 gataacgatt cctttgcctc caacctcgct tagtgcacgc aaaatctcag caatttgacg
    24901 tacaccatta agacgccgtt cttcgataaa gtcaccgatc cacccatcgt agccgttaca
    24961 agctgtcact actgggatcc ctgtttcttt gattgcctgt ttgactgtgt cgatattttc
    25021 tattaacaac ttaccgtcga tttcgtattc ttcaaatcca cagtctttga taaatctaaa
    25081 tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct
    25141 cctcctcgga cttgaacgcc cattttgata ctttcttctg ggtgctggtc tacataacgc
    25201 atgtaagctt ctgcgctttc tgcaaatggc acaactggat caatgatttc ttcacaatcc
    25261 aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga ttcgtttacg gttccatctt
    25321 gggtaatctg ggtttggttc actagatgct cgagcaaaga cgatttttgc attattgaaa
    25381 tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt tagcaaaggc aacataagaa
    25441 atgatcccgc catagccgat ccctattaaa gaagcttgta atgcggcagc actgccactc
    25501 gtttcaataa ctgaatctac tccacgttta tctgtcaatt cctttagacg aaaaccgaca
    25561 tctgctttca taggatctag cgtgtaatcg gcaccgtgtt tttctgctaa ttctctacgc
    25621 tgcgctatgg gatcgatccc aacgatggta gtagcacctg cttttttagc taattgaatc
    25681 gcaatttgcc cgatagcgcc taaaccgaag attgctacag catcacctgc tcgaacatgt
    25741 gcttcccgga taccacctaa agcgaattga gcaggatcgt aacatactgc atttttccat
    25801 tgatccaacc gatcaagttt acgtagttta taattatcaa caccgtttgt aatcagcgtt
    25861 tcacgaatgg gtccataagt gcagactaac tctccttttt gatactctgt caccaaactg
    25921 cccgtttctt cgatcgtacc gacaatcata tttcctaatt gaaattcgcc aaataccaca
    25981 ccgcgttggc ttccttcttc tcttggcaag aataggcgcc attcttctga aaattcttca
    26041 tcgataaatg gacttaatcc tctaaagtcg acaatttctg ttcctggttt tggagtagcg
    26101 aaaattactc ggatacgtac ttcgttcttt tcgatctcac ggtcttcgta atctttgaat
    26161 ccggctattc ttggttctaa tgcaactaat tttttcatct tttctttcct ctttcttttc
    26221 tttttatcct tttacgccgc cttcgactaa atcaccttta ataaatcgtt cagataacgc
    26281 atacataatg atgactggta aagcggtcac caatgaagcg gccatcattc gtccccaaat
    26341 gtagtctggt gtactgaata atgtgttcaa accgattggt aaagtaaatt tttcggaggt
    26401 cgacaggaaa atcgaagcaa ataaataatc attccacgaa accataaagc aataaacgaa
    26461 gacagaaatg attccagaaa tagccaatgg tataatgatc cgaataatga tctggatccg
    26521 gctaagacca tcgatcattg ctgcttcttc aatatcagtc ggaatcgtgt caaaataact
    26581 ttttagcatg aatatcgcag taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt
    26641 attggtatca tacaaaccta gaccggaaat gattttaaat aatggaacaa cgatcaaaat
    26701 accggaaaac atataaacgg tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg
    26761 tagttttgac aaagcgtatg ccccgctgat cccaatcact actgcagtta cggaggctat
    26821 caaggagatc actaaactat ttttgaaata catgatgaat ggaaaaatct cagcattgaa
    26881 aatatcctga taatgttcaa aagtccattc tttcggaaaa atagtttgtg tcgttgcaat
    26941 tgcttcttgt gaatccttga atgatgtcat cagcatgatc aaaaatggaa acaaattgac
    27001 agctaatagc aacaaaatcg tcgcgtaaaa taagattttc ctgctctttt tatttttcat
    27061 ttcaagtcca ccttctttcg tgagagcaag acgaccgctg caataatgat gaacaataga
    27121 atcgatatag ctgctgcttt tccaagatca ttgaacgcaa aggctgtctg atacaaataa
    27181 acgcctaaga tattgacttt attcgtcagt agatagacat ctgtaaacat atagaacatc
    27241 cagatagatc gtaaaacaat cacagttgct agtactggtt tgattgccgg cagtgtcacg
    27301 atcttgaact tctgccaagc atttgcaccg tccatttcag ctgcttcata aagcgttttg
    27361 tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat acggatagta tctccagata
    27421 gcaaacaaaa cgaccataaa gaagctagca actggctggt cgaaccacag cggtgcttcg
    27481 ttgaaaagct gcaacctatc tactaacaga tcattgacga ttccataact attattgaac
    27541 atatatttcc aagcaaaaat caaagaaata gatggcgtaa cataacttaa taaaatcagt
    27601 gaacgcaccg ttttcctaaa tctgaacttg cggttaagca aaatagctac ggctaatcct
    27661 aaagctgtac tgcctgctac aactaagaat gtgtaagcaa ctgttaaacc taaagaacga
    27721 taaaattcac tatcacttaa aatttcaaga taattgctta atccgataaa aacggcgtct
    27781 gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa tattggaaag cattggaatc
    27841 agcactaaaa cagctaataa aagcatgctt ggtgctaaaa gtcccatggc taattttaag
    27901 tcagaatgtt tccttggacg attcattgaa acctccccct gataatctgc caatagctat
    27961 ctactttttt cgaacaccat tggcagaact tcttgttatt tcgtattttt ttgtgcgtca
    28021 gctaaagctt gatcgacatc ttcgtttccg acagttactt ggttcactgc ttttccgatc
    28081 acacctgagc ttgtgatatc tcccattttc gtgaaattct tttccccgac tagtccaaag
    28141 acttgtactt catcaaaagc actagcaatc tcagatggca gctcgccaaa agacttgatg
    28201 acttcatttt ctttgtaacc atccagttcg actacttgtt tattcactgg ttgagcacca
    28261 ccaggagaca tcaagaccca cttttccatg tttttcggtt gagataagaa agctgtgaat
    28321 gcttcagcag cctctttttg tttctcgtct agaccagaag aaatcgtcaa tccagatact
    28381 gtgccataga cagcttgatc tttttgtgtt gggatagcaa aaccgatatt ttctgagttc
    28441 cccgcttcat aaacagaagg caaaatgtaa gttgaataga ttgccattgg tgctgttcca
    28501 ttcatgaaag catctttgat ttctgtcaca tcattagagc ctggcatcgt gtattgggat
    28561 aagttttgat aaaacgacaa tgcttctctc atcttttccg tatcgatcgt tacctcgcct
    28621 tcatcatcca aaacatttgc tccatttgac aaagcaaatt gagagaacgc ttgctcactc
    28681 attgttcctt cagcagtagg catagcaatt ccatattgtt tattctcttt atctgtgaaa
    28741 tgttgagcaa tttttaaaat atcatcccaa ttttctggtt ctgaaaaacc agcttctgat
    28801 aatttttctt tgtcatacca aataccttga acccagccgc taattggtgc agcaatatag
    28861 ctttttccat cttctgaacg aacaaggttt ttggctccgt cataataatt atcttctcca
    28921 gcttcctcga taacgttttg gactgccgtc tgatcaatca gttcatcttt atccatcact
    28981 ttagcaaaat cctgactgac ttccattact tctggtaatt taccggaacg agctaacgta
    29041 acgatttttg tattgaacgc tgattcttct accggcactt gcttgatttt gatgtctgga
    29101 ttttcttttt caaaatcagc aaccagttca ttgatcacac ttaatcgttc ctgttcgaca
    29161 gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt tagagctttg actttcacca
    29221 caagcagtaa gaagaaatcc agtagctagc agacaggtag taacaataat ttttttcatt
    29281 tttttccacc tcttatttta ttattttcag ccacatatat tcatacggct ggaatatcaa
    29341 ctttcgttct tgactgcaat ggataatttg tccagaaaac aggtctaggt aatctccttt
    29401 tggtaaaaga taacctgctg attgattgga aaaattatgg ataactaaga ctttctcttc
    29461 cttcgcttcg cgaataaaac tgataaaaga atctgctgag tccagtaagt aaatcaaact
    29521 attcggatgg aaggcttttt ctttttttcg aatagatagc atctgggtca attcaccata
    29581 tactgctgtt cttaatggat ctgtctgcaa ctctttctca atatctgcta acaaaaattt
    29641 ttgccgattg atttttcgat tctctcctgt tttttctacg cctttgtaat catttcgact
    29701 tcccagcaca ctttgaatat aaattgccgg cacgcctggt aacgttacca aaaatgaatg
    29761 agcagctaaa aagcgtttgc ttctccattc cgtcgtatct gttggagaac ttaacgcatc
    29821 tagatacgta gcgtttatct catatggact ggtcgttcca tctgggtttt tcttgtagtt
    29881 tactcgggca ccctctttct tcagctgttc cactaattca aggatcgttt tttccggcag
    29941 aatacctcgt accggattca atccgatacc gtcatgggag gctaaaaaat taaaaaaagt
    30001 tgtttttttc gatgcgaaat cgatggtttt taaccattct ttcaacttac ttgtattacc
    30061 cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc
    30121 attctcgcca ttcccaaaat aactgatatt atctttgtgt ggcacatttg tctcagtgat
    30181 caataccgtt cctggagctg cttcatcagc gattgcacgg aataattgga tgattttatg
    30241 cgtttctggc aggtgtatac aagaagttcc tagttctttc cataaaaatc ccacagcgtc
    30301 caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa agaatatcga tcattttcaa
    30361 taaaacttct ggatttttaa aattcaagtc tacttgatct tcgctaaaag tcgtccatac
    30421 gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct
    30481 agtcacgctg cttgtatctg tatccagtgg caaatcaata aaaaaatctt tgtacgtatc
    30541 attcaagttt aaatattcct gaaaccaagt actttcggca gacatatgat tgcagacaaa
    30601 gtcgaacatt aaccgtgttt cgcttctcat ttcttctata tcttcccatg tgcccaaacg
    30661 gtcgttgatt gcctgataat ttacaacgga gaaaccatca tcagatgtat atgggaaaaa
    30721 tggcaagaaa tgtacaactc taaatgtatc tgccaaatac ttttgataaa atttcttaaa
    30781 tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat
    30841 atcttcttcg tcccaatgca gtttgcctgc ctggcctttc ttctgctgat aaacactgac
    30901 tttttgttca atcatagagg cggttttctc agacaaatct gagtcgcgat aaatcgcttg
    30961 taaaagtttt gtatacatcg gattcccctt tcttttttgg gagcgttccc aacgatttga
    31021 tcatattatg gaaaacgctt actgtcaata gcattttttt aaataatttt gatatacaca
    31081 aaaatacaaa taagaaagct tatgtatcaa taattaatag cttcttaaaa aaatatggtc
    31141 tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta
    31201 ttgtactatg gaattattct aaaacgctcc caaaaaggag gtgcatataa aattgaaacc
    31261 tactatttat gacattgcga aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt
    31321 aaataaccag ccaaatatct ctgataaagc aaaagcaagt gtacttcaag caatcaaaga
    31381 attgaattat gaaccgagca aaattgcaag aggactgtca ggaaatggtt ttgacgcgat
    31441 tctagcatta tctcatcgta cttctcatac gacctatggc aatccttttt tttcagatgt
    31501 tattcaatcc atttctgccg tttctgaaca aaataacttc gacttgattc tacaaacggc
    31561 aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg
    31621 aggaattatt attttaagct ctacgatgga tgaaacactt ctttttgaat tggatcaata
    31681 caatattcct attgtggtaa tcggaaaagt cggaaacgac tacacgaaca tctactcagt
    31741 ggacacggat aatttccagg atagttacga tttggtggaa acactgatca agaatggaca
    31801 ccgtcatatc ggctgtctcc atgctccgac agaagtttat gtatccaatg atcgtctcaa
    31861 tggttaccgt caatgcttat ttgatcacca cctagatatc cgtaatgaat ggattataga
    31921 cggcggcttt cgcttagaag atagcatggc agcaatgaat gatttaatga agctacgaga
    31981 aagaccaact gcgatttttt ctaccgatgc attaaaatca ttgagtattt acaaagtggc
    32041 agaacaatct ggaaagcaga tacctgatga tctttcgatc gcaggattca gtgatccctt
    32101 attgtcctcc ctattttcgc cacagttgac tcggatagac attccgacaa atcagttagg
    32161 aatcaaagcc acagaacttt tattccaact aatccaccac caataccctg aagaaaaaag
    32221 aatcattctt tcaaccgaga ttactctcac agattcgatc caacaacaag aaatgtagaa
    32281 cagaattata aaagaacaga aacactgagg ataagtcagt tactctgttc ttttttcaat
    32341 cactcatccc tcgttacccc ctcccggtat tttagcgaat ttttcttatt ttccatctgt
    32401 tttagatagc tgtggataac tataatgttt gtgttaaact attttttcca cagggaagaa
    32461 aaaactattt ttaaccacgt tgtgtataag tttttatagt tcgttttttt aaacacattt
    32521 acccttattt caatccatgt tatttgggga tacttatctc taaaacataa atagaataac
    32581 agacatgtct actggttttg ttttattcga ggaattattc aataatagct agcaaaatat
    32641 taaaaataac cgctctatct tcccatcaat ttattttgca ttcaaaaaat gatctatctt
    32701 tacttttgtt gattgacttc tcttggaaga tgatcgttcc gataccttaa ttggatacga
    32761 accgatgttg ttaagatcct ttcccacttt tcttctagtt agacaaatta ttttagacaa
    32821 accaaaaaag aggttgtgac aataatttgt cacaacctct tttttaaaat atctattttg
    32881 tttgattgtt cattgcgtct tctgcagcca tttgcgcttc aatatctgaa atactgtaga
    32941 cattttcgct tgctacaccg acttcttttg gttccatgtc acgataacgt tgcataccag
    33001 taccagctgg gatgatctta cctataataa cattttcttt caaaccaaga agaggatctt
    33061 tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt ttcttggaat gatgcagcag
    33121 ataagaaact atttgtttct aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag
    33181 ttgcaggaac gcctcctgag actagcgtat tgtagttttg atctttaaag tctgcgatgt
    33241 ctaataatgt acctggtaag atatctgtat cacctggatc cattacacga actttacgca
    33301 gcatttgacg aaccattact tcgatatgct tgtcgccgat ttctacccct tgcatacggt
    33361 aaacacgttg tacttcacga agcaagtagt tttcaacaga taatacatcg cgaacttgca
    33421 acaattgttt tggatcgatt gatccttctg tcaatggtgc accacggtgg atcatatcgc
    33481 cttctgctac tttcatacgt gctgtataag gaacagtata cgtacgtgta tctgtttttc
    33541 ctttgattgt tacttctttg gtacgtgttg caggatcttc tgagatatcg atgacatccc
    33601 ctgttacttc tgtgatcact gcttgccctt tcggattacg tgcttcaaag atttcttgga
    33661 cacgaggaag accttgagtg atatcgtctc cggcaacccc acctgtatgg aatgtacgca
    33721 tcgtcaactg agtaccaggt tcaccgattg attgagcggc gatcgtacca actgcttctc
    33781 cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta acaatgtttg cagacaccat
    33841 gttttgtatt acatgtgaag acagaacgga ttgtcactgt ttcgacacct gcatcaacga
    33901 tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt
    33961 caggatgacg tacttcttta cgggtgtaac gtcccactaa acgatcttct aatgattcga
    34021 tgatttcatt tccctcgcgg atggcttgga tttcaagacc tcggtcagta ccgcaatctt
    34081 cttcacggat gattacatct tgggccacgt caaccagacg acgagtcaag taacctgaat
    34141 cggcagtctt caaggctgta tcggtcatcc ctttacgagc cccgtgagtt gagataaaca
    34201 tttccaagac ggatagtcct tcacggaagt tcgaaatgat tggcaattcc atgattcgac
    34261 cattaggagc ggccatcaaa ccacgcattc cggcaagctg tgtaaagttg gaaatgttac
    34321 cacgagctcc agaatcagac atcatgaaga ttggattttt cgcttccaaa ccttccatca
    34381 gtttttgttg gatgctgtct tttgcagcat tccaaacagc aatgacacgt tcataacgtt
    34441 cgtcatctgt gatcagacca cgacggaatt gttttgtaat agtttcaact tgtttatggg
    34501 cattttcaat gatttcttgt ttttcattca gtacactgat gtcagcgatc cctactgtga
    34561 taccggcatg tgtcgaatgt ttgtacccta agtctttcat acggtcaagc atcttagaag
    34621 tttctgttac tttgaatcgt ttgaagactt ctgcgatgat gtttcctaag tttttcttct
    34681 tgaacggtaa tactaattct tgctctttga tatgtgcagg aatatcagta ccagcttcaa
    34741 caaagtattt gtccggtgtt tggacggtca agttgaagtc tgttggttca ttcagataag
    34801 ggaattcagg aggcatgatc tcgttgaaga tgatcttacc aactgttgtg atcatcgtac
    34861 gttctttttg ccattcagta aatggttttt cacctaatga gttagggttc acaccgatac
    34921 gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac ggcttcgttt tgatctctga
    34981 agaccattcc ttcgccttcg cgtccttctt cttccattgt caagtagtag ttacctagaa
    35041 ccatatcttg agaaggagta acaactggtt taccatcttt agggttcaag atgttttgag
    35101 cagctaacat caacatacgt gcttctgctt gtgcttcttc attcaatgga acgtggacag
    35161 ccatttggtc tccatcgaaa tcggcattat aagcttcaca tacaagtgga tgcaaacgga
    35221 ttgcgcgtcc ttgtactaaa actggttcga atgcttggat accaagtcgg tgaagcgtag
    35281 gtgcccggtt cagcaatact gggtgttcct tgattacgtc ttctaaaaca tcccatactt
    35341 cgtcttcttg acgttcgatt ttacgtttcg catttttgat gtttgaagct agttcgcgtt
    35401 gaacaagctc acgcatcacg aatggtttga ataattcaat ggccatttct tttggaagtc
    35461 cacattggta catcttcaag aaaggtccaa caacgataac ggaacgacct gagtagtcga
    35521 cacgtttacc tagcaagttt tgacggaaac gaccttgttt ccctttcaac atatgagaaa
    35581 gagatttcaa tgggcggtta cctggtccag taactggacg tccgcgacga ccgttatcga
    35641 tcaatgcatc tactgcttct tgcagcatac gtttttcatt ttgaacgatg attccaggtg
    35701 cattcaagtc taacaaacgt ttcaaacggt tattacggtt gattacacga cggtaaagat
    35761 cattcaagtc acttgttgca aaacgtccac cttctaattg aaccattggg cgtaaatctg
    35821 gtggaataac agggataacg tccataacca tccagcttgg ttgatttcct gaagcgcgga
    35881 atgcttctaa aatatccaaa cggcggattg cacgtgtacg tttttgtcct tgtgcagatt
    35941 tcaattcttc tttcaattcg gctgcttctg catctagatc aacattatca agtaattgtt
    36001 taatcgcttc tgcacccata gcagcttgga attcttgtcc atattgttca cgtttttcgc
    36061 gatattcgcg ttctgtcaac aattgttttt tctctaaatt cgtatttcct ggttcgatga
    36121 ctacatatga agcaaaataa atgatttctt ccaaagcgcg tgggctcatg tctaatacaa
    36181 gacccatacg tgaaggaatc cctttaaagt accagatatg tgaaacagga gctgctaatt
    36241 caatatgtcc catgcgttca cggcgaactt tcgaacgagt aacttccact ccgcatcgat
    36301 cacaaacgat tcctttataa cggatacgtt tgtattttcc gcaggcacat tcccagtctt
    36361 ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg ttcaggtttt aaggtacgat
    36421 agttaatcgt ctcaggtttt tttacttcac cgtaagacca gcttctgatt ttttcgggag
    36481 aagccaaacc tatttgcata ctttcgaatt tatttacatc gatcaaaagg ggttccctcc
    36541 atttcattta atggcaaacc gcaagacggc tgatcttcga ccgccttgca gctcatagac
    36601 cggattagtc taaattatct tctgcggttt caaagttttg gatgacatcg tctttttcat
    36661 cttccacttg ttcagcagct ttcttttcta attctttggc agtttgctgt tcggcaaatt
    36721 ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga
    36781 tctctgtttc ttcgatatcc aagacacgca tgtccagacc tagtgattgc aattctttca
    36841 ccaatacgcg gaatgattcc ggtacacctg gttttggaat tggttcaccc ttgacgattg
    36901 cttcgtatgt tttcacacgt ccgactacgt catctgattt gtatgtcaag atttcttgta
    36961 aagtataagc cgcaccgtaa gcttccagtg cccatacttc catttcacca aaacgttgtc
    37021 caccaaattg agctttacct cccaatggtt gttgtgtaac caatgagtaa ggtccgattg
    37081 aacgtgcatg caatttgtca tcgaccatgt gagcaagttt gatcatgtac atcacaccga
    37141 cagacacacg tccatcaaat ggttcacctg tacgtccgtc gtacagaaca gttttcgcat
    37201 cgctggccat accggcttca cgaactgttt cccatacgtc ttcatcactt gcaccatcaa
    37261 atactggcgt tgctacgtgg atacctaact gacgtgcagc catacccaag tgtaattcca
    37321 atacttgccc gatgttcata cgtgaaggca cccctaaagg attcagcatg atatcgattg
    37381 gtgttccatc aggtaggaat ggcatatctt cttccggcat aatacgggat acaacccctt
    37441 tatttccgtg acgaccggcc attttatccc cttcatggat tttacgtttt tgaacgatat
    37501 aaacacgaac taacatgttt acgcctggtg acaattcatc tccagcttca cgagtaaaga
    37561 ttttcacatc gtgaacgata ccgccgccgc catgtggtac acgtaatgat gtatcgcgga
    37621 cttcacgagc tttttcgcca aagatcgcat gcaatagacg ttcttctgca gataattctg
    37681 ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc atcttggact tctgcaccaa
    37741 tacggatgat acccatttcg tctaaatctt ttaatgcgtc ttccccgaca ttagggattt
    37801 cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc ttctgattca tattcttcaa
    37861 tatggataga agtgtatacg tcatctttta ccaaacgacg gctcatgatg atcgcatctt
    37921 catagttgta cccttcccaa gtcatgaaac caactaaaac gttttggcct agagccattt
    37981 cgccttgttc catagatggt ccgtctgcca gagtatcccc tttttcgact ttctcaccca
    38041 aatgaacgat tggacgttgg ttgtagcttg ttccggagtt tgaacgacgg aattttgtta
    38101 catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt
    38161 attcaacgac accgtcatgt ttacataaca aagcagctcc tgagtcatgc gctgatttgt
    38221 attccatacc agtaccaacc catggtgctt gaggattgat caacggcact gcttgacgct
    38281 gcatgttggc acccatcaag gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg
    38341 ctgtcgcaac ggcaactact tgtttaggtg atacgtccat gtagtctact ttatcaatag
    38401 atacttctaa gttttcactt tgagcacgag ccataacaac gtcttgagca aatgttccat
    38461 cttcattcaa tgggctgttt gcttgggcta cgatgtaatg gtcttcgata tcagctgtca
    38521 agtagtcgat ttggtcagta acacgacctg tttcacggtc tacacgacga taaggtgttt
    38581 cgataaatcc gaatttattt actttcgcat aactggacaa actattgatc aacccgatgt
    38641 ttgggccttc aggagtttcg atcggacaca tacggccata gtgggagtag tgaacgtcac
    38701 gaacttcata accggcgcgg tcacgagtca atccgccagg tcctaaggct gaaagacgac
    38761 gtttgtgtgt caattcgcca agagggtttg tttgatccat gaactgtgac aactgagaag
    38821 aaccaaagaa ttctttgata cttgccacaa ctggacggat attgatcaat tgttgaggtg
    38881 tcaatgtttc agtatcttgg atcgacattc tttcacgaac cacacgttcc atacgtgcta
    38941 gcccgatacg gaattggttt tgtaaaagtt caccgactga acggatacga cgatttccta
    39001 agtggtcgat atcatcgaca ttgccgatac cttccatcaa gttcaagaaa tagctcattg
    39061 aagcaatgat gtcggctgga cgaaccgttt ttacagctgc ttctggatag ccgtttccga
    39121 tcacgtttac ttcacgttca gggtctcttg gtgaaaatac tttgatgact tggacagtca
    39181 ttggatctgt gaccacaccg tcttctgatg gatagtaagt cactgaattt aggccattat
    39241 caatgaaagg agctagtgtt tccatgactt ggtgagtcag aactgttcct ttttcaacga
    39301 tgatctcgcc tgtttctgga tcaactaatg tttcagctaa tgtcagattc aatagacgtg
    39361 ttttcaaatc taattttttg tttactttgt aacgaccaac gtttgctaag tcgtaacgtt
    39421 ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc
    39481 ctggacgcaa acgttcgtaa acgtctttca aaccttcttc tgttcttgag tcgcttgcgt
    39541 ttttatgcaa gtctttttcg actgtattgc gtaatgtttc gctgtcgccg aagatttcaa
    39601 agatcgtatc atcggaacca aatcctaacg cacgaactaa tacggttaaa ggaatcttac
    39661 gtgtacggtc gatacgtaca taagagatgt cttttgcatc agtttccatt tctagccaag
    39721 ctccacggtt agggataaca gtagaaccga atccttcttt tccgtttttg tcgacttttc
    39781 catggaaata gacacctggt gaacggacta actgagaaac gattactcgc tctgccccgt
    39841 tgatgatgaa tgttccttgt tctgtcatta atgggaaatc accgaagaat acttcttgtg
    39901 ctttgatttc gccagtctcg cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt
    39961 agtttgcatc atgcgcacga gcttctgcta ctgtatactt tggttctttc aattcataat
    40021 ctacaaattc caaagataga ttgccattga agtcatcgat tggcaaaatg tcttcaaaca
    40081 tttctctaag tccctcatct aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat
    40141 ttggtaattc caacacttca ctgattcttg cgaaacttct acgttcgcga tgttttccgt
    40201 attttactac gtgtccagcc aagctcttca cccctctaaa atgtcattca aaaagactac
    40261 ccgatcaatt gttacaataa tatttcaaat attattttta ggtaacaaca gacatttttt
    40321 gcggctttta ggcaaaaaaa aaccaaaaat agaacattct cttgaaacct tacttcgtga
    40381 gcttctactt ttgtttcctt atattaaagc cgaaacggac aatatttcgt ttctgccttt
    40441 tcttcatcag atagtttttg caacttcata tactacactt attttctata aaagtcaaca
    40501 cttcctggcg tttcttctgc tttttcgcaa cattttctta atgttttcaa ttttatgttt
    40561 tcaattatct gacaattctt aattgacaga cctttttatc tcggttatac tttagaaaat
    40621 tcattttgtg gaggaacttc tatgcgaacc aacatcaacc aaatcgcacc aacacaactg
    40681 aataactttt actttagcta ctttagatag gtttgtattc atcatgatgg atacaaacgt
    40741 taaacgacag tttgtatcta tgatggctaa agaactttgt attgcagcaa aaagccacgt
    40801 agacaaaatg ctaagtggct gagcagtgaa aatacaaagt aaaagtatca agcattctac
    40861 tttttcaatt tattttccga cttttcaatc catttggaca ttttgtctga atggattttt
    40921 tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg
    40981 ctgccctgct actagcttta ggaggttgcg cacaaaaaga caattccgca gaggcatcag
    41041 atggacaaaa aacaattggc gtattgcaac ttgtacaaca taactcgtta gatgctgcct
    41101 ataaaggatt caaagaagga ttagcagaag aagggtataa agaaggcgat aatctaacaa
    41161 tcgattatca aaatgcccaa aacaatcaag acaacctgaa aagtatgagt gagaaactcg
    41221 tcaaagaaga tcctgatttg ctacttggaa tcgccactcc agcagcacaa tctttattaa
    41281 atgaaacaac cactatcccg atgactgtca cagcggtcac agacttggaa caagcccatt
    41341 tagtcacatc caatgaaaaa cctggcggca atgtgactgg cacgacagat atggtgccaa
    41401 tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga cgccaaaaca atcggcatca
    41461 tgtataacaa cggtgaagca aactctaaga tccaagctga tttggcagaa aaagaattga
    41521 aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc cacaaatgat gtccaacaag
    41581 tgacgaaaag tctagctaaa gatgtagatg gaatctatat tcctactgat aacacgttcg
    41641 caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg
    41701 gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt aggaatcgac tacgaaagct
    41761 tgggcaaaca aacagggatc atggcagcaa aaatcttaga cggcaagaat ccaagtgaac
    41821 ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa taaagacatg gccaaagcat
    41881 taggcatcaa tccagacagc atcaaagaag ctgaataaaa cacaggagga ttctcatgac
    41941 aaatttgctc tctcttttct tatcatctgc atcgcaaggg gtcttatggg ccctacttgc
    42001 gattggtgta tacgtcactt tccgtatctt ggacattgcc gacttgactg ctgaaggaag
    42061 ctttccttta ggtgcaggga tcgcggcagt aagtattaca aatggctatc atccattgac
    42121 agcttgtctt ctaggctttt tagggggtgc ggcagcaggg cttgtttccg gactgcttca
    42181 cacaaaattg aaaattcccg cccttcttgc agggatcatc accatgactg gtttatattc
    42241 tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta ttgggtcagc caacgatctt
    42301 tcattgggtc gagtcattcg gcataacaaa agacaacgct gtactgatcg tcggtctgct
    42361 ttttgctttg atcgttgtat gtttacttgt tttgttctta cgtacagaaa caggacttgc
    42421 tattcgtgca actggcgata atctggcaat gagcgaagca aacgggatca acacagataa
    42481 tatgaagatc atcggttata tgatttctaa tggatgcatt gctctttctg gctccttact
    42541 tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt ggaacgatcg taattggact
    42601 tgcttctatt atcatcgcag aagttttgtt acgtaaccag ccactatttc tccgtctctt
    42661 gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta gcgttagtct tcgaactgaa
    42721 tgttgaacca agtgattcga aacttgcttc tgctttcgtg ctagtgatct gtctttcttt
    42781 cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg
    42841 aatgacgtct taacattgga aaatctccat catacctttg aaaaaggaac gatcaacgaa
    42901 aatcatgtcc tgaaaggcat taatctttcc ttagatcccg gcgacttcgt gacgatcatc
    42961 ggtggaaatg gggctggtaa atctactttg ctgaacagca tcgccggtac attttctgtc
    43021 gatcagggtc aaattttatt gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt
    43081 tcgaaatcca tcagtcgcgt attccaagat cctaaactag ggacagccgt tcgtttaacg
    43141 gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt
    43201 gtcaaaccac aagaccgaac atttttcaaa gaccagctgg ttcggctaaa tttggggttg
    43261 gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag gacaacgtca agctattacg
    43321 ttattgatgg ccacattaca gcgtccagaa ctgatcttat tagatgaaca cactgcagcg
    43381 cttgaccctg ctacttccaa aacggtgatg gaattgacag aaaagctgat cactgagcag
    43441 aatctcactg cttttatggt cactcatgac atggaagacg cgatccgcta tgggagtcga
    43501 ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc agcaagagga aaaacagcag
    43561 ctcacagtgc cccaattatt agcgttgttc aaacaaaaca gcggcacggc attaaaagac
    43621 gatcaagtac ttttatcttc ataatcagaa agaggatggg acaaaaaatg aacgcccacc
    43681 ctcttctttt ttctctttcc ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc
    43741 gaaaactatt ttgttctctc cttcttcctg atagaatgga tgggaaagga gcttgatgcc
    43801 atgacaacga aagacaaggt acttcaatta ttgaaaaaga caccagattt tctttccgga
    43861 gaaaagttgg cacagcaact ggaagtttcc cggacaagta tttggaaagc catcaaagaa
    43921 ttggaaaagg caggctatcg ttttgagcac gggcctacag ggtatcgtta cctgccctct
    43981 gacgtactag atgcaaaaga aatccagcaa gagctacggt cgtttattcc tgatttgaaa
    44041 gtacaaacga tgttgacttc tgaatctacg atgaaagatg ccaaactggc agcagctcaa
    44101 ggagattctg tttcgaagtt atttgttgct gatacacaag aacaagctcg tggacgtttt
    44161 ggacggcctt ttttcgctga acctggacga ggaatctaca tgagtctctt gctccatcca
    44221 gacaaacggt ttgatgaatt gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa
    44281 gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat gggtcaatga tatctacatg
    44341 gatggaaaaa aagtctgtgg gattttatct gaagcagtca gtgatatgga aagcggcagg
    44401 atcagtcata tcatcattgg gatcgggctt aatttttctg ttcctcaaaa tcattttcca
    44461 gctgatctcc aagaaaaagc catctctgtc tttcccgacg gacaagctag tgtcacaagg
    44521 aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt atctttctca cccagaaaaa
    44581 gcgttggact tctataaaga aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa
    44641 aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc
    44701 caaacaaatg aacagaagat gagtttgtct tctggcgaaa tcagtctcac atctatccgt
    44761 acgcaataat tatcagattt cacaaaagga cacctgatct catacagtgt ccttttttct
    44821 gaattctctt caaaagactc tccttattct atcagctgac agtttctcta tcctctagaa
    44881 aaaggtattc taaaaatatc tataaagcat ggtacacttt ctttaaatga atcaatgcaa
    44941 aaaacaagag agtcatcagt ttacacgatc aaaagagata gcaggccgct tcctttttca
    45001 atcttataga tagaccatgg gtaacgataa aaacaaaaaa gaacttgcga cttattttgc
    45061 cgtaagttct tttttagcta gtgccttaat ctcttaatcc caacaaacgt ctcgcataga
    45121 tcacctgacc agagtgcata gcagcatcat caataataga aacgagacgc attcctcgtg
    45181 tgactggtgg tgtccagttc tcgtcaatta cttcatctag ctcggaaggt tccagccttc
    45241 ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta tcctagcaga tcttctattt
    45301 gattagcttg gatctgcttt gcttgttcca gactatgctt ccaatcttgt tcgttatctg
    45361 ctatcgtcat cggaaaccgg ctttcccagt cttgactatg ccaaattgga gaagttcctg
    45421 ctaaatcagc aatctggaaa tctaattctc gagcggtatg ccaagccagc caagtcattg
    45481 atttgatctg cggggctcta tcttccactg gaaaacgatt ggcctcctca atagagacgc
    45541 cttcaaacaa acgttcaaac cgatcgtatg cccgatccac actctcgcat agcatatcta
    45601 aataatccat ttattttcct cctttttatt tatcctactt actttttcct aattctagga
    45661 tacctactta tatgttacta cacaccctta ttttttacac tttaacagtc accatcatca
    45721 atagtttgga gattgaaaga aaaagatagt gtcgaactat caattgaaca agaagcaaca
    45781 atatgaacgt ttcattaata ttctttaaat aaacaatgat ttttcaaacg aacagtttaa
    45841 gactaggaga aaaagagtat aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga
    45901 aaaaaagggc aagtttacta gtcctagttg ctttgttgtt caatatcttt tcgtatggtg
    45961 tggccgtttc agcggaaaca tacattccta aagaccctgg gaatatttat aatgatctaa
    46021 ctggtaacta tggcttgtta ggaattgcta gtcaattcca cgtatttgct aaaaataaga
    46081 caactgtgaa tgcacataca gacggaaata tagcaacaca tgaacttgat gcaaaaaata
    46141 attttggtac agagcttcat gaaggtttat tgaaccaaga aataaattat atacaaaaaa
    46201 ctgattcttt cgctaattct tcaggcatac ctacaggtag cacaagaatg aataaatttg
    46261 tcgttggcag taacgtggat gtagcacttc aagatggtca tcctacagtc aatggatcca
    46321 ggcttgatca cttaactcca gatgattttt ataaagatcg tgctgggcat gaatatattg
    46381 atttcgagca ggaatttgct aaattgaatg tagcagcaaa tgatcttgca acgatcactc
    46441 cagctaaaac atatactgct gcagattttc cagacatgaa caatcggaca atcgatttaa
    46501 ctgatttatc agataccggg tttttattgg taaatatcga tgcagaagtg ttaacgatga
    46561 atacgccact tcaaatcatt aatccaaatg atcaagtagt tgtatttaat gtcatcaaca
    46621 gttcttctgc attgaacgta cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa
    46681 cagaagattt ttcggatgcg aatatctctt ggaattttgg aaatgagatg actgatttga
    46741 caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa tgctactatc cgcattactc
    46801 aaaaccaaga cggaacgatt ataggaacaa atgtgatttt agatgcagct accaatcgtt
    46861 gggatcctaa cgaaatattt atcagcgaca atggtacaga tacgacagac acaagcgaca
    46921 gtacagatac atctaccaca agtgatagct cagattcatc a
//
LOCUS       DAFEZB010000016        42532 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFEZB010000016
VERSION     DAFEZB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.016"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            476..1339
                     /locus_tag="LP859_000925"
     CDS             476..1339
                     /locus_tag="LP859_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3028896.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LP859_000926"
     CDS             complement(1468..3090)
                     /locus_tag="LP859_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028897.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LP859_000927"
     CDS             3339..3860
                     /locus_tag="LP859_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL3028898.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LP859_000928"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LP859_000928"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL3028899.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LP859_000929"
     CDS             5941..6759
                     /locus_tag="LP859_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL3028900.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LP859_000930"
     CDS             complement(6862..7059)
                     /locus_tag="LP859_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028901.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LP859_000931"
     CDS             7228..7806
                     /locus_tag="LP859_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL3028902.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LP859_000932"
     CDS             7901..8038
                     /locus_tag="LP859_000932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028903.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="LP859_000933"
     CDS             complement(8851..9354)
                     /locus_tag="LP859_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL3028904.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="LP859_000934"
     CDS             9713..10738
                     /locus_tag="LP859_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3028905.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="LP859_000935"
     CDS             11085..11963
                     /locus_tag="LP859_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL3028906.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="LP859_000936"
     CDS             12439..12555
                     /locus_tag="LP859_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL3028907.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..13821
                     /locus_tag="LP859_000937"
     CDS             13483..13821
                     /locus_tag="LP859_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028908.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13915..14145
                     /locus_tag="LP859_000938"
     CDS             13915..14145
                     /locus_tag="LP859_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028909.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14545..14916
                     /locus_tag="LP859_000939"
     CDS             14545..14916
                     /locus_tag="LP859_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL3028910.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15190..15882)
                     /locus_tag="LP859_000940"
     CDS             complement(15190..15882)
                     /locus_tag="LP859_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3028911.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15885..16670)
                     /locus_tag="LP859_000941"
     CDS             complement(15885..16670)
                     /locus_tag="LP859_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3028912.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16907..18307
                     /gene="lacG"
                     /locus_tag="LP859_000942"
     CDS             16907..18307
                     /gene="lacG"
                     /locus_tag="LP859_000942"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL3028913.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18332..18649
                     /locus_tag="LP859_000943"
     CDS             18332..18649
                     /locus_tag="LP859_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL3028914.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18662..20335
                     /locus_tag="LP859_000944"
     CDS             18662..20335
                     /locus_tag="LP859_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL3028915.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20349..21980
                     /locus_tag="LP859_000945"
     CDS             20349..21980
                     /locus_tag="LP859_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL3028916.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22852..24042
                     /locus_tag="LP859_000946"
     CDS             22852..24042
                     /locus_tag="LP859_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBL3028917.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24176..26365
                     /locus_tag="LP859_000947"
     CDS             24176..26365
                     /locus_tag="LP859_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3028918.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26362..28155
                     /locus_tag="LP859_000948"
     CDS             26362..28155
                     /locus_tag="LP859_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL3028919.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28355..28669
                     /locus_tag="LP859_000949"
     CDS             28355..28669
                     /locus_tag="LP859_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3028920.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28690..29067
                     /locus_tag="LP859_000950"
     CDS             28690..29067
                     /locus_tag="LP859_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3028921.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            29077..29850
                     /locus_tag="LP859_000951"
     CDS             29077..29850
                     /locus_tag="LP859_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028922.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29872..31275
                     /locus_tag="LP859_000952"
     CDS             29872..31275
                     /locus_tag="LP859_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL3028923.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31457..32641
                     /locus_tag="LP859_000953"
     CDS             31457..32641
                     /locus_tag="LP859_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3028924.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32638..32928
                     /locus_tag="LP859_000954"
     CDS             32638..32928
                     /locus_tag="LP859_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028925.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32925..33146
                     /locus_tag="LP859_000955"
     CDS             32925..33146
                     /locus_tag="LP859_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028926.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            33188..33967
                     /locus_tag="LP859_000956"
     CDS             33188..33967
                     /locus_tag="LP859_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBL3028927.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            34034..34528
                     /locus_tag="LP859_000957"
     CDS             34034..34528
                     /locus_tag="LP859_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3028928.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34584..35222
                     /locus_tag="LP859_000958"
     CDS             34584..35222
                     /locus_tag="LP859_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028929.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35294..35689
                     /locus_tag="LP859_000959"
     CDS             35294..35689
                     /locus_tag="LP859_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3028930.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35673..38126
                     /locus_tag="LP859_000960"
     CDS             35673..38126
                     /locus_tag="LP859_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3028931.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            38123..40150
                     /locus_tag="LP859_000961"
     CDS             38123..40150
                     /locus_tag="LP859_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028932.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            40147..41169
                     /locus_tag="LP859_000962"
     CDS             40147..41169
                     /locus_tag="LP859_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL3028933.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            41186..42115
                     /locus_tag="LP859_000963"
     CDS             41186..42115
                     /locus_tag="LP859_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3028934.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42360..42476
                     /locus_tag="LP859_000964"
     CDS             42360..42476
                     /locus_tag="LP859_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL3028935.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT        13920 a         7051 c         8397 g        13164 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13861 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    13921 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    13981 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14041 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14101 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14161 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14221 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14281 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14341 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14401 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14461 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14521 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14581 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14641 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14701 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14761 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14821 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    14881 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    14941 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15001 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15061 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15121 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15181 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15241 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15301 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15361 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15421 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15481 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15541 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15601 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15661 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15721 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15781 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15841 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    15901 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    15961 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16021 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16081 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16141 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16201 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16261 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16321 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16381 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16441 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16501 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16561 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16621 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16681 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16741 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16801 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16861 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    16921 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    16981 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17041 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17101 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17161 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17221 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17281 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17341 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17401 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17461 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17521 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17581 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17641 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17701 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17761 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17821 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    17881 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    17941 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18001 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18061 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18121 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18181 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18241 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18301 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18361 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18421 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18481 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18541 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18601 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18661 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18721 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18781 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18841 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    18901 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    18961 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19021 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19081 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19141 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19201 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19261 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19321 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19381 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19441 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19501 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19561 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19621 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19681 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19741 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19801 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19861 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    19921 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    19981 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20041 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20101 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20161 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20221 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20281 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20341 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20401 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20461 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20521 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20581 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20641 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20701 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20761 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20821 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    20881 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    20941 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21001 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21061 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21121 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21181 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21241 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21301 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21361 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21421 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21481 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21541 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21601 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21661 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21721 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21781 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21841 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    21901 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    21961 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22021 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22081 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22141 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22201 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22261 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22321 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22381 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22441 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    22501 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    22561 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    22621 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    22681 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    22741 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    22801 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    22861 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    22921 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    22981 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    23041 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    23101 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    23161 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    23221 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    23281 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    23341 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    23401 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    23461 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    23521 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    23581 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    23641 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    23701 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    23761 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    23821 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    23881 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    23941 tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa
    24001 ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg
    24061 aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt actaacataa
    24121 tttatgataa acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt
    24181 tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga
    24241 taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc
    24301 attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga
    24361 tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt
    24421 tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc
    24481 tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat
    24541 ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga
    24601 attagactct catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt
    24661 ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt
    24721 aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg
    24781 ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct
    24841 tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac
    24901 aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt
    24961 aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca
    25021 actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc
    25081 agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt
    25141 agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt
    25201 tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca
    25261 tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac
    25321 tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat
    25381 gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa
    25441 ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag
    25501 atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg
    25561 agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga
    25621 cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt
    25681 gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc
    25741 aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa
    25801 agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac
    25861 aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc
    25921 aattatagat gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca
    25981 aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa
    26041 ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta
    26101 tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa
    26161 aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc
    26221 tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt tattagagta
    26281 ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat
    26341 actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat
    26401 ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg
    26461 agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga
    26521 tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa
    26581 cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac aatacatcag
    26641 ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga
    26701 tttacaatta ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg
    26761 aatttaaatc tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt
    26821 aaaattaatg aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa
    26881 aataaagaaa ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac
    26941 agtaacgttg cacgtaatat tgctagtaat ataagggcga tttatgtgga tgaatttcaa
    27001 gatactagag aattacaata caatgtaata gctaaattac ttcaaaacaa ccctcaaatt
    27061 cagtcaatgt ttgttggaga tattgaccaa gcaatttatg gatcattaga tggtatagct
    27121 aaatcagttg aagaactaga aatgctaact agtcacactt ttcaatcaaa gacgctacat
    27181 ggctgttaca ggtctaatca gagattagta gacttctact ctaattttca gagttgtcct
    27241 tacgagattg aaagcagagg gaataataaa aacgaccgtg gttttataag ttatgattgc
    27301 aaagttacta aggaggattt acccaacata ataaaaaata taataaagga aaagattgaa
    27361 agcggcattc atgaaaaaga tatttgtgta cttgctcctc aacactatcc tctgttttca
    27421 ctgggtgagt atttaaagaa agaattaccg tattgcaatt ttgatgctat aaatatatca
    27481 ccaattaagg taaataatca tagtttattt ttcaagttag caattttgta ctttactgaa
    27541 tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg atattttaat tatattaaaa
    27601 aatgaatttg gaatagaagt taaagattat tttattgact tagatttatt acagctaata
    27661 aacagtgtga aaagaatgta tagtggagac gacaacggtg ttgatttata caaatttgtt
    27721 accaaacata tatttgttaa tcttaatatt gacgagaaaa attacccgtc tatttttaag
    27781 cactatctat tttttattaa cgaagtagaa gatcggataa aaaataattc attatctagt
    27841 agtgtatcta gttttaagaa gatgtttaaa tctcgagatg gtatcacact tactacagca
    27901 cataaagtta aaggaaatga gtatgatact gttatcgcta ttaatcttct aaacgggaaa
    27961 attcctcatt ggaatgaaat ttttaatgtt tctgataaag gaattagttc agcgagaaaa
    28021 ctactatacg taatttgttc aagagcaaaa aataatttat ttcttttctc agaggtgggt
    28081 tattttacga gtaaaggaga cttgctttat cccactcaat tattagataa aatagaattt
    28141 gattataata tttaaaatac ctaggtagtc gttgttttaa atatagtagg aatcggacgc
    28201 tcagtacgga gcgtctattt ttttacccaa ttttgaaagg aagtgaactt atgaaaataa
    28261 aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac catcacacaa ataagattca
    28321 ttcaaacata aaaacagaaa ggataggtaa aaatatggaa cttaaatatg tcattcccaa
    28381 tatggaaaaa acatttggac aattagaata tgctggtgaa gggaacattg aacaacgtcg
    28441 aataaatggc agaccaacca ttctctctcg tagttacaat ttatactcaa cgattcaacg
    28501 agcagatgat attgttgtca ttcttccctc cgaagctggc gagaaacatt ttgaatccga
    28561 cgaaaaagtc aaactcatca acccacatat taccgcagaa ggttataaaa ttggcaatcg
    28621 aggatttacc aattacatta tgcacgcaga cgatatggtg aaagcataag aaaggaaagg
    28681 tgaaaaaata tgagattagc agaaggtatt gtgattgaaa aagaccagac atttggcaca
    28741 ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga atgaagatgg tacggtttcc
    28801 gaagaaatta aagaacgtac ctatgactta aaatcaaaag gacaaggacg catgatacaa
    28861 gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact acaacgcaga agtggaatta
    28921 atcaatcctg tggcagatac cgtagcgacg gctacttttc gaggggcaga cgttgattgg
    28981 tacgtgaaag cagacgatct tgtattaaaa ggacagacaa aacctcatca gaaatataaa
    29041 aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga caaacacacc attgattttg
    29101 aaagaaattc cgaaggatga agccatttct tttattcggc aataccatta ttcaaagata
    29161 ctccctagat tgtgtaaata ctttctaggg attttttcag aagaaaaatt attaggcgtt
    29221 gtggaattag gctggggtac gcaaccccta caaaccatac gcaaactctt tcccgacagt
    29281 tcccttcaaa ccacagatta tttagaaatt ggaaagatgt gttttcttcc agaaatgaat
    29341 caaaccaatt attttggcag tcaagctctt tcagctctaa ttaagtggtt gaaagaacat
    29401 acggactgtc attttttata tacactggca gatggaattg agggaaaatg tggctatgtc
    29461 tatcaagcgt ccaacttctt ttactgcggg tatttcaaga caagtgtcta tcgtgataag
    29521 cagtcatggg aaaagataca ccctcgaagt gctcgccttt tattagagga aaatgctcgc
    29581 tttgaacagg tagagaaaaa gcattggctt tcccaggcat tttgcgaata caaagggatt
    29641 gagaaaatca atggacgcat gtttcgctat ctctatccgt tgacaaaaga agcgaaaaaa
    29701 ctgttaggac acactctcta tcgacgtcat tattatccga aagaaaaaaa tttacgcttt
    29761 gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc aaccaacttt tgataaacag
    29821 gctcgtatct acaatacaca attattttga taggtggaaa ggaggatttt tatgccattg
    29881 cttcatgaca gaggagagcg aatcaaagcc aacgataagc acttggtcta tcattttgta
    29941 ctaatttcat tgctggtagt ttttctagtc gtcctactcc cttttcattg gagagcgtta
    30001 caatcggtga actggcaagc agttcagtta gatacatttt tctatcaact tcatacgcca
    30061 tacttttgga gcagtattct cactgctctt tttgtatgcg gacttatcag ctggctttat
    30121 tatcactatc gtatagattc tataaaacag ttagagcacc gtcaaaagtt agctcgtatg
    30181 attttagaaa atcattggta tgaagccgaa gaaactcaat cagagggatt ctttaaagat
    30241 ttacccagca agaaaaccaa acaacgaatc cgccattttc ctaaaatata ttatcgaatg
    30301 aaagatggac taatccatat tcatgtagaa atcaccttag ggaaatatca agatcagctt
    30361 ttgcacctag aaaacaaact agaaacagga ttgtattgtg aattagtgga aaaaacgttg
    30421 aaagattctt atgtagagta tgtcctactt tatgatacta ttggcaatcg aattggcatt
    30481 gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg aatcggtcta ttgggagttc
    30541 gattctctcc ctcacatgtt aattgctggg ggcactggta gtggaaaatc ctattttatt
    30601 ctcacattga ttgaatctct cctgcataca aatgcaaagc tttatattct tgaccctaaa
    30661 aatgctgatt tggcagactt aggggcggtc atgaataatg tctattaccg taaagaagat
    30721 atgttggcat gtatcaatca attttacgaa gatatgattg atcgcagtga aaccatgaaa
    30781 caacacccta actataaaac aggagaaaat tatgcttatc ttggtttgtc tgccaatttt
    30841 cttatttttg atgaatatgt ggcaatgatg gatatgctag gtcgtgaaag taccaccgtt
    30901 attcataaac tcaaacaaat tgtaatgtta ggacgacaag caggcttctt tttaattctt
    30961 gcctgtcaac gtccagacgc aaaatatttt agtgatggga ttcgggacaa ctttaacttc
    31021 cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta tgatgttcgg aagcgataca
    31081 caaaaacaat ttttcttaaa acccatcaag ggaagaggtt acgtggatac aggaaaaagt
    31141 gtgattagtg agttttatac accccttgta ccaaaaaggt acgatttctt aggagaaata
    31201 ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc acgaagtgaa aggtacaggt
    31261 tcagacttgc tttgattggt gtggcgttag ccacgccaat cctttagccc cctctatcta
    31321 acaggggggc acaaatacaa ctgaaaacag gcaattctca tcaaccaatg ttgatggtac
    31381 aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt catagtttac atctttttta
    31441 gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt aaggaattga aagaaaagcg
    31501 actggcttat ggcgtttctc aaaataaact ggcggttgct tctcatatta ccagacctta
    31561 cctatcggat attgaaacag gcaaggcagt ccctactcaa caggtgaaag aagatttact
    31621 gaacgctttg gaacgcttca atcccgataa cccattggag atgttattcg actatgtacg
    31681 gattcgattc cctaccaatg atgtggctca aattatcggt gaggttttac gtttgaatat
    31741 ggattatatg ctacacgaag actttggcta ttattcttat cccgaacact atcgtttcgg
    31801 tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt gttttattag aactgaaagg
    31861 aaaaggctgt cggcaatttg aaaactttct attggctcaa catcggagct ggtatgattt
    31921 ctttcaagat tgcatggaac ataaaggtat tttcaaacgc ttggatttag ctatcaacga
    31981 taaaacaggg attttgaata ttccagattt ggcaaagaaa tgtaaacagg aagaatgcat
    32041 ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa ttggttcacc gtgatgaaaa
    32101 accatacatg ggaaacacac tttatattgg ctcattaaaa agtgaagtct atttctgtat
    32161 atatgagaaa gattatgagc aatacgtgaa aaatgatgtt cccttagaag acgcagaagt
    32221 aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc acccaagcca tgaaagattt
    32281 attggcacat caacaggcgg aaaaaacagc ctttgaaatt atcaatcgct atatccgctt
    32341 tgcagataaa gacgacacta aacgtcggag tgattggaaa actaatgaac gttgggaatg
    32401 gtttatcggt aaaaatcgtg gagcattacg cctaaccaca cagccagagc cttattcttt
    32461 tgaacgaaca ttgaactggt tacatcatca agtcgcccct acattaaaaa tcgcaagcat
    32521 tttagatgtt ctcaacggta caaccattat ctccaccatg attcaggaag ccaaactgac
    32581 agaaaaacat gagaaattaa ttgaacaaca acatctagcc atggaggatt tgattacatg
    32641 agccttttaa caaaagaatt aaagaagtta gggtttcagt gtggcattga gtttcaagcg
    32701 tatatacaaa atacagggaa atatacgtct ctcattattg agggaaaacg gcaagctggt
    32761 gataccatct atacctatga tttttacaag gtgagatttt ataataatta taccaatcga
    32821 gtaaccgtct atggagagca tttaacccct tttcaattat taaggcgtgt aaaaagctat
    32881 atctattatc gagaaaaata tcttaaggaa aggaggacaa tcacatgaat tttgggcaga
    32941 atttgtacaa ctggttcttg gataatgcac agtcattggt attgttagcg attgttgtca
    33001 ttggactgtt tttaggtttt aaacgagaat tttcaaaact cattggcttc ttgattattg
    33061 ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt gaaagatgtg ttattaaact
    33121 tatttaaccg aatcattggt gcttaacaga tatttttcat gataaaatag gaagtatata
    33181 ttttgttatg aaaggattta tgaatatgtt tacaaatgat caatttaaat ctcttttaat
    33241 taaaacatta gaaagaaaga gctcttcaaa ttattatgaa ggaggtaacg agattgtttc
    33301 taaaatgaaa atatcagagg taactttaga tgagactgac gattttactt attataaagg
    33361 atataaaaag ggctggaata cgctttgcac atatcttaaa ataagagttc cttttgatga
    33421 tttagacttt tttgaaagtc ataaagattt gattacccag acagcaagct ccatatatga
    33481 taaacaaggt gataatgtat tagtggatac tattttagta ccacttccag agaattacga
    33541 agtaattaac tttagtcagt tgaaaattag tgatgtggtt tcacaagcta ttgaagacgc
    33601 tgagtctttt atgagtaatg gtgaatatca acgtgccttt gacagagttc acactgcttt
    33661 tcatggatat ttaattgaaa tactaaaaaa atatgaaatt acagttccta gagacgaaaa
    33721 tctatctaaa ttatatagta ggatacaaca attaattgaa aaagaaattc aacctactga
    33781 acttgctgat atagtgaaga caacgataag gtcatcaaat ggaatgatta gctctcttaa
    33841 tgaagcaaga aataggcatt cacttgcaca ccctaataca aatattattg gaaagagaga
    33901 agcaaaacta ataataggta tttcaagtac agttacagat tatattagcg gataccttga
    33961 taagtagcgt cattaactga ttgctacttt ttttatgact atttttataa aaaggatgtg
    34021 ataagaattg gaaatgaaag tctatgtggc aaatttagga cggtataatg aaggtgaatt
    34081 agtcggagca tggtttactc caccaattga tgaggaagaa atggcagaaa gaatcggttt
    34141 aaatgaggac tacgaagaat atgcaataca tgattttgag cttccttttg atgtggacga
    34201 atacacgccg attagtgaaa tcaaccgtct atgtgaagcc attcaagaaa tcgaaggtac
    34261 acctatttat aatgaactca aagaaattca aggcatgtgg tttagtagct tggaagaatt
    34321 actggaaaac aaagaggata ttcattgtta tagtgattgt gattcgatgg aagatgtcgc
    34381 ccgttattac gtggaagaaa caggacagct tggtgaagtc ccttccaatt tacaaaatta
    34441 tattgactac caatctttgg gacgtgatat ggaaatagag ggtaattacc tagttacctc
    34501 tcatggggta tttgaatatt gtcagtaagt ttaacaatcc gtgttataat taaattaata
    34561 aatagcacgg agaggtataa attatggtaa atgtatttga aaatcttaaa ctattaaaac
    34621 tagacatgaa agataaaggc tgggtgatag attcctttta ctttcgttac aaacagcaaa
    34681 attatattgt acttgttaag ctatttgaaa aagaggaaaa agttccagaa tatgctctgt
    34741 taaaactaga atttttaaag gaaaacgatt tttcagatat gctagctgtc tatgctaatt
    34801 cggtaaaatt atatacagac acgaaaacaa taagagaata ttttggtatc gaatacagta
    34861 gtaacttggg agatgtttta tttcaattta gccaaactct tgctcgattt attccgacag
    34921 aagttagtga gaagaaaaat gaagaccaaa aagaagccat gtgtttctca ttaagtcaaa
    34981 gtgattcaga agatcccaga aaaaaatatt gtttttcagt tagaagaaat cctttaaagg
    35041 gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa taaaacgaga ttgtatagac
    35101 ccagcttata tgaacgatta gggtcagata ccaatttaag tttcagatat tcaatgaatc
    35161 cagatgatga agaaactgat gaaggtatta ttgcaaaatg gacaaaaaat aaaattgaat
    35221 agttaaagca ttgaaagagc aatcacctat tttgattgtt ctttttctat ttttacggaa
    35281 aggacgtgaa gacatgaaaa aaataagaag ttacaccagt atttggtcag tagaaaaagt
    35341 gatttatgcg attaatgatt tacaactccc tttcccaatt acctttacgc aaatgacatg
    35401 gtttatcgtt tctttgtttg tcgtcatact ctttggcagt ttaccaccac tttctctgat
    35461 tgacggagca ctactcaaat acttagggat tcccattgtt ctcactgggt tcatgtgcca
    35521 aaagaccttt gacggaaaga aaccctatgg atttctaaag tctatcatta cctatgctct
    35581 acgccctaaa gtaacctacg ctggcaaaca aatacgatta aaaaaagaaa caatgaatga
    35641 atcgattacg attgtaagga gtgaacaact agatggcata tccgattaaa tatatggaag
    35701 ataatctcgt atttaatcat gatggcgaag tattcgctta ttatgaatta attccctata
    35761 attacagttt tttaagttcc gatgaaaaaa ttcaagttca tgataatttc tgtcaattga
    35821 ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact agcaaccgaa tcaagtattc
    35881 gagccacaca ggaacaatcg aaaaaagaaa tcactggacg attaaaagag atagcttacg
    35941 aaaaaataga tgaacagacc gaagcgttaa tttctatgat tggtgacaat caagtagact
    36001 atcgcttttt tattggtttt aaactctacc tcaatgaaca ggaagtatcc gtaaaaaatg
    36061 ttgggaaaga aattcaaaaa gcatttgcgg aattctttca tggtgtgaat cacaaattaa
    36121 tgggcgattt tgtttctatg cctatggcgg aaatcgaacg cttttctaag atggaacagt
    36181 tgttagaaag taaattatcc agacgctttc agattcgtaa attggagaaa gatgattttg
    36241 gttatattat tgaacatctt tacgggcaaa caggtgtccc ttttgatgac tacgactacc
    36301 atttaccgaa acgatacgaa gataaaaaag tgttggtaaa gaaatacgat attctcaaac
    36361 ccactcgctg tttaatcgaa gaaaatcaac gctatttacg gattgaacag gaagatacca
    36421 ctacttatgt ggcgtatttt acaattaata gtattgttgg tgaattggat ttccctaata
    36481 gtgaaatttt ttattaccag caacagcagt ttactttccc cgttgatacc agtatgaatg
    36541 ttgaaattgt cgccaataaa aaagccttat ctaccgttcg caataaaaag aaagaactga
    36601 aagatttaga tagccacgca tgggaaagcg acaacgaaac cagtactaat gtggtggacg
    36661 ctttagaatc tgtcgatgaa ttagaaagta atttagacca aaccaaagaa tctatgtaca
    36721 aactaagcta tgtggtacgg gtatcagcaa agaatttgga cgagctgaaa caacgttgtg
    36781 atgaagtaaa agatttttat gatgatctgt ctatcaaatt agtacgacct tttggcgata
    36841 tgctaggctt gcataatgaa tttattccct caagcaagcg atatatcaat gactatattc
    36901 aatatgtcac gtcggatttt ttggctggtc ttggtttcgg tgctacacaa atgattggtg
    36961 aaaattctgg tatttacgtc ggttacaatc tcgatacagg cagaaatatt tatctccaac
    37021 ctagccttgc aagtcaagga attaaaggct ctgtaacaaa tgcattagcg tccgctttta
    37081 ttggttcatt aggtggcgga aaatcattct ctaacaatat gctggtctat tattcagtac
    37141 tctttggcgg acaggcaacc attgttgacc caaaagctga acgtggtgaa tggaaagaaa
    37201 ccttacctga tattgcccat gaaatcaata ttgttaatct cacgagtgag aacgacaata
    37261 aagggttact tgacccttat gtgattttaa aactcccgaa agattcagaa agccttgcca
    37321 ttgatattct cacgttctta acaggtattt ccagccgaga tagcgagaag tttcctgtcc
    37381 tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg agggttgtta ttggtgattg
    37441 atgaattgcg aaaagaagat acgcccatca gcaatagtat tgctgaccat atcgaaagct
    37501 ttgtcgatta tgattttgcc catctgttat tcagtgatgg cagtgtcacc caatccatta
    37561 gcttggaaaa acaactcaat attattcaag tggcagattt ggtcttaccc gattccaata
    37621 gctcttttga agaatacact accatggaat tattaagtgt ggcaatgctg attgtcatta
    37681 gtacctttgc tttagacttt atccactctg accgttccat ttttaaaatt gttgaccttg
    37741 atgaggcttg gagtttttta caagtggcac aaggaaaagc tctatctatg cgattggttc
    37801 gagcaggacg tgccatgaat gcaggggtct atttcgttac tcaaaacgcg gacgatttac
    37861 tagatgaaaa gatgaagaat aatattggct tgaaatttgc ctttcgttcc actgacctag
    37921 tggaaatcaa aaagacctta gaattttttg gtgtggacaa ggaagatgaa aacaatcaga
    37981 aacgcttacg tgaattagaa aatggacaat gtttagtttc agacatctat ggtcgtgtag
    38041 gggttatgca gtttcacccg atttttgaag agttgcttca tgcctttgat accagaccgc
    38101 ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag aaatttcttc actatagttg
    38161 gataacgctc ctttgtgtgg gcgttatttt acttttatta ggcacgctgg gcaatgcagt
    38221 acaagcgaca ggcttagttg atgaaaccat tgatacaagc aatgaatact ccaagtatgg
    38281 tttgaatcaa tatcagcttg attattatgt ggacaatagc tggggctggc ttccatggaa
    38341 ttggagtgat ggcataggac aatcggtgat gtatggattg tatgccatta ctaattttat
    38401 ttggacaatt tctctttatc tatcgaacgc cacaggttat ttggtgaaag aagcctatgc
    38461 cttagatttc attagtcaaa catcccaagc aattggcgaa aacatgcaga cgattgcagg
    38521 cattacaccg aatggctttt ccagttcagg gttttatgtc ggtttcctcc ttctttttat
    38581 tctcattgtt ggaatctatg ttgcctatac aggattggtt aagcgagaaa cgacgaaagt
    38641 gatacgagct gttttaaact ttgtcgtggt gtttattcta tcggcttcct ttattgcgta
    38701 tgcacccgat tatattgcga aaatcaatga tttttccaaa gatgtcagta ccgccagtct
    38761 gaatattggg acaaaaattg ttcttcctaa cacggatagt caagggaaag acagtgtgga
    38821 catgatacgt gacagcttat tctctgttca agtaaagcaa ccatggctat tgcttcaata
    38881 tggctccact gatgtagaag cgttagggga agaacgtgtg gaaagtttat tgtctaccag
    38941 tccagataca aacaatggtg aagatagaga aaatgtagtg attgaagaaa ttgaagatca
    39001 tgacaatacc tatttaaccc taccgaagac gatttccaga ttaggaacag tcttcttttt
    39061 gtttgtattc aatattggta tctcgatttt tgttttcctt ctaacaggaa tcatgatttt
    39121 ttcgcaagtg ctatttatta ttttcgctat gtttttgcca atcagtttcc tattaagtat
    39181 gttgccgagt tttgatggca tgggcaggca tgccattacc aagttattta atgtgattat
    39241 gacaagggct ggtattacct tgattattac cgttgccttt agtatttcca ctatgcttta
    39301 ttcgctgtca gcaagttctc cctttttcat gattatgttt ttacaaattg tgacctttgc
    39361 agggatttat tttaaactgg gcgatttaat gagcctattc actttacaaa gcaacgactc
    39421 tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa atgatgaatc gacaaaccag
    39481 aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa gcagggtcaa cgaagcaaaa
    39541 gacaatgaca acaaagcaga atcaaaccaa taaagctaac catactcgtc aaaatgaacg
    39601 gactacacca aagaaagaaa cagcactttc caatgtgaaa aagcaacaag gcggtatcgg
    39661 gcaacgatta ggtgaaaaga cagcccatgt attggacacg aaaaatcgtc tagtcaataa
    39721 aagaaagcag gcaaaacaac agatacaggc gacaccaacc catgtcaaat atgcccttca
    39781 tcaaggaaaa gagaatgtca ctcataatgt aacagacttt aaacaatcta tggcagatac
    39841 caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag caaaaacgaa agaacattgc
    39901 tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga cgaaaagaaa acccatcatc
    39961 tcctgttcag aataaccagc agcatgttcg caagcgtcag caaaccgtca cgcctgtttt
    40021 gtcacaaaac actaaacgtg aaaatcatca gcgaccattg aaaaaagagc aaaaacaaaa
    40081 gacaccaccg aagcaattca atcagaggaa gctaaataaa agaacggata agcgaggaca
    40141 acaaacatga agctaaaaat aacagtgatt tgtagtgtgg tcttctctct cttctttttt
    40201 ctcttattat tcctagtcat tttctttgca gatgatacgg acagtggcga aaacaacaag
    40261 gattcttcta ttccacaagg cggtgtgacc gtttcaccag aagtgttggc acaccgtccg
    40321 ttgattgaaa aatatggtaa ggaatatggg attgaagact atgtttctta tatcttagcc
    40381 attatgcaag tggaatcggg cggtacagca gaagatgtta tgcaaagttc cgaatctttg
    40441 ggtttaccgc ccaacagttt gagtaccgaa gagtccatca aacaaggggt taaatatttc
    40501 agtgaattat taaccagtgc agaacaacaa ggagtggata tagattctgt tatccaatct
    40561 tataattatg gcggtggctt tttaaattat gtgaggagtc atgggaaaaa atatacctat
    40621 gaattagccg aacagttttc taaagagaag tcgggtggtc aaaaagcaga ctatcccaat
    40681 cctatagcca tacctgtgaa tggtggttgg cgatataact atggaaatca gttttatgtt
    40741 cagttggttt cacaatacct aaccgacact tccccaacag aatttgatga tgaaaccgtg
    40801 caagtcatta tggacgaagc cttgaaatac gaagggtttc cctatgtctt tggaggggcg
    40861 tcgcctacca cctcttttga ttgtagtggc ttaatacaat gggtgtatga caaagctggc
    40921 atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta cgcaagaaat ctctatggaa
    40981 gaagcccaag caggcgacct tatcttcttt cattcaacct ataatgcagg cacatatgtg
    41041 actcatgtgg cgatatattt agagggaaat cgcttttatc atgcaggcga cccaatcggt
    41101 tatggcgact taagcagtcg ttattggcaa gaccatctga ttggggctag acgtgttatt
    41161 cataactaag aaaggaatga atcaaatgat tataaaaatc gagcgaaaag aaaagaagga
    41221 gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa gttccgatga ttaaagttgg
    41281 cacgcataag aaattaacct ttgttttatg ggttttattg attggtagtg tcggatttgg
    41341 aatctataaa aattttactg ccattgacac tcatactgtc cgtgaaacag aaatcattaa
    41401 acagcagatt gtcgatacca accaagtaga aagttttgtg aaatcctttg cgaaagacta
    41461 cttttcgtgg caacaatccc aagaagccat tgataaacga aatgaaaaat tgacccatta
    41521 tttgacggaa gaattgcagg tattaaatga agaaatgatt cgtaaggata ttcctactag
    41581 ttctagtgtg aacgatatac aggtgtggca ggtttctcaa gtgaatgaga acacctttga
    41641 ggtactgttt tctgttgaac aagttattac cgaagacaaa gacaaggaaa ctatatcgtc
    41701 tagctttcat gtggttgtcc atatagatga atcagataat atggttatta tcaaaaatcc
    41761 gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc aaacagctag aaagtgacca
    41821 tacagtagat acagaaacga tggacgaaat cactagcttt ttagaaacgt tcttccagct
    41881 ctatcctacc gccacagaaa aagaattgac ttactatgta agtaatcatg tgttacctat
    41941 gattaacaag gaatatgttt tcgaggaatt ggtgaatcct atctttacca gaaaagataa
    42001 ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa gaaacaaagg caacccaaat
    42061 ctcgcaattt gagttgatat tagaaaaaca agataattgg aagattgtaa aataacaaat
    42121 attggtacat gattacagat actttgtaat catgtactct ttttgataaa aaattggaga
    42181 ttcctttaca aatatgctct tacgtgctat tatttaagtg actatttaaa aggagttaat
    42241 aaatatgcgg caaggtattc ttaaataaac tgtcaatttg atagcgggaa caaataatta
    42301 gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac ctttatcatg
    42361 tgattctaaa gtatccagag aatatctgta tgctttgtat acctatggtt atgcataaaa
    42421 atcccagtga taaaagtatt tatcactggg atttttatgc ccttttgggt ttttgaatgg
    42481 aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat gc
//
LOCUS       DAFEZB010000017        40827 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFEZB010000017
VERSION     DAFEZB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.017"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            35..658
                     /locus_tag="LP859_000965"
     CDS             35..658
                     /locus_tag="LP859_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028936.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1108..2157
                     /locus_tag="LP859_000966"
     CDS             1108..2157
                     /locus_tag="LP859_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3028937.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2154..3035
                     /locus_tag="LP859_000967"
     CDS             2154..3035
                     /locus_tag="LP859_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028938.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3038..3808
                     /locus_tag="LP859_000968"
     CDS             3038..3808
                     /locus_tag="LP859_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3028939.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4087..4941
                     /gene="aroE"
                     /locus_tag="LP859_000969"
     CDS             4087..4941
                     /gene="aroE"
                     /locus_tag="LP859_000969"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL3028940.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5119..6282
                     /locus_tag="LP859_000970"
     CDS             5119..6282
                     /locus_tag="LP859_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3028941.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6399..6809
                     /locus_tag="LP859_000971"
     CDS             6399..6809
                     /locus_tag="LP859_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3028942.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6983..9211
                     /gene="topB"
                     /locus_tag="LP859_000972"
     CDS             6983..9211
                     /gene="topB"
                     /locus_tag="LP859_000972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3028943.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9382..9666
                     /locus_tag="LP859_000973"
     CDS             9382..9666
                     /locus_tag="LP859_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028944.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9826..10062
                     /locus_tag="LP859_000974"
     CDS             9826..10062
                     /locus_tag="LP859_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3028945.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10059..10457
                     /locus_tag="LP859_000975"
     CDS             10059..10457
                     /locus_tag="LP859_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL3028946.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11124..12068)
                     /locus_tag="LP859_000976"
     CDS             complement(11124..12068)
                     /locus_tag="LP859_000976"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL3028947.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12364..13308)
                     /locus_tag="LP859_000977"
     CDS             complement(12364..13308)
                     /locus_tag="LP859_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028948.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13529..14743
                     /locus_tag="LP859_000978"
     CDS             13529..14743
                     /locus_tag="LP859_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3028949.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14765..16780
                     /locus_tag="LP859_000979"
     CDS             14765..16780
                     /locus_tag="LP859_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL3028950.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16863..18236)
                     /locus_tag="LP859_000980"
     CDS             complement(16863..18236)
                     /locus_tag="LP859_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL3028951.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18262..18546)
                     /locus_tag="LP859_000981"
     CDS             complement(18262..18546)
                     /locus_tag="LP859_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3028952.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18548..20563)
                     /locus_tag="LP859_000982"
     CDS             complement(18548..20563)
                     /locus_tag="LP859_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3028953.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20742..21047)
                     /locus_tag="LP859_000983"
     CDS             complement(20742..21047)
                     /locus_tag="LP859_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL3028954.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21136..22167)
                     /locus_tag="LP859_000984"
     CDS             complement(21136..22167)
                     /locus_tag="LP859_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3028955.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22374..23249
                     /locus_tag="LP859_000985"
     CDS             22374..23249
                     /locus_tag="LP859_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3028956.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23401..24243)
                     /locus_tag="LP859_000986"
     CDS             complement(23401..24243)
                     /locus_tag="LP859_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3028957.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24335..26212)
                     /locus_tag="LP859_000987"
     CDS             complement(24335..26212)
                     /locus_tag="LP859_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3028958.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26209..27630)
                     /locus_tag="LP859_000988"
     CDS             complement(26209..27630)
                     /locus_tag="LP859_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3028959.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27633..31022)
                     /locus_tag="LP859_000989"
                     /pseudo
     CDS             complement(27633..31022)
                     /locus_tag="LP859_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            31279..32670
                     /locus_tag="LP859_000990"
     CDS             31279..32670
                     /locus_tag="LP859_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3028960.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32743..33390)
                     /locus_tag="LP859_000991"
     CDS             complement(32743..33390)
                     /locus_tag="LP859_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL3028961.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33398..34084)
                     /locus_tag="LP859_000992"
     CDS             complement(33398..34084)
                     /locus_tag="LP859_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3028962.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34090..35163)
                     /locus_tag="LP859_000993"
     CDS             complement(34090..35163)
                     /locus_tag="LP859_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3028963.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35385..36059
                     /locus_tag="LP859_000994"
     CDS             35385..36059
                     /locus_tag="LP859_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3028964.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36064..37404
                     /locus_tag="LP859_000995"
     CDS             36064..37404
                     /locus_tag="LP859_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3028965.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37553..38539)
                     /locus_tag="LP859_000996"
     CDS             complement(37553..38539)
                     /locus_tag="LP859_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL3028966.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39263..40294
                     /locus_tag="LP859_000997"
     CDS             39263..40294
                     /locus_tag="LP859_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3028967.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40403..>40827
                     /locus_tag="LP859_000998"
     CDS             40403..>40827
                     /locus_tag="LP859_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL3028968.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12679 a         7413 c         7663 g        13072 t
ORIGIN      
        1 ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg aagtagttaa
       61 atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa atccaagtaa
      121 gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag ttgcaatgtt
      181 tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt cgtccatacc
      241 aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca aacaaagccg
      301 ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca tgcttgatgt
      361 ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt atccgatcgt
      421 ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag tgacatttat
      481 tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag gaataactga
      541 ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata tcaaacaatt
      601 agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg ctaattgaga
      661 cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca gaacaagtga
      721 tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga aattcaaaaa
      781 ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac ttatcaaaac
      841 gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt gtattaatgg
      901 tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt atctatgcta
      961 gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg taaagacaat
     1021 gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta taataaataa
     1081 aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac agttgtagta
     1141 attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg cgaaaaagaa
     1201 agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca atacgtgagc
     1261 catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga agaaggatta
     1321 aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca gagcaagcta
     1381 gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg aatcgcaaca
     1441 cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt aggtgctgta
     1501 acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg taatgtaaca
     1561 ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga tttgttgcca
     1621 aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa ataccaagta
     1681 gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata tgcggtgcct
     1741 tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga ttttatctat
     1801 gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga agcaaacaaa
     1861 gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg actagcaaca
     1921 atcgggatca atcaatatca attagggctt caaagcggta agatggcggc agaacttgcg
     1981 agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg ggatacagtg
     2041 atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat gaaggaaaaa
     2101 gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga caaatgatcg
     2161 tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt ttcatgactt
     2221 atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta ggtgctgctg
     2281 tctgtgtgac agctattact catggggtag cgccgattgt agctacatta ttaggcgtag
     2341 gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga aagataccgg
     2401 ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg tttgtgatgc
     2461 gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc catcgctttg
     2521 ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc atcttgattg
     2581 ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg acaggagaca
     2641 acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt ttaggtttaa
     2701 ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac gacggatatg
     2761 cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc atcatcggtg
     2821 aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt gtcggcagta
     2881 tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg acttatctga
     2941 agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg aagaaagcac
     3001 tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat tgacaatcaa
     3061 aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat tggatgaaat
     3121 taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg gtgctggaaa
     3181 aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat cgatctcttt
     3241 taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc tttctcgagt
     3301 tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa atttgctact
     3361 tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa atcaaaaaga
     3421 agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact tggatactcc
     3481 agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg cgacgatcac
     3541 agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta aaacagctaa
     3601 agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt gtctcatgat
     3661 cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac tccaaaaagg
     3721 gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg gcgacctttt
     3781 gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc agtagaagga
     3841 cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa taaacaatta
     3901 aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt aaatagaata
     3961 ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca cctagacgtt
     4021 tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga acaaaggagg
     4081 aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc ggcttctcac
     4141 agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga tgcggtgtat
     4201 ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat tcgaactttt
     4261 gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc ttatctggat
     4321 gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca cgaaaagggt
     4381 cgtttgattg gctataatac tgatggtgcg ggcttcatgc aatccgtgca agaagctggg
     4441 atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc aaaggcagtc
     4501 actgttcaag cagcgttaga cggcgctgag gaaatcgtag tttataaacg aaacaaccaa
     4561 acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc ttgtaagata
     4621 aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga aagctgtctt
     4681 ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc gctcttagat
     4741 ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc tagagaaaca
     4801 cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact tggcatgttg
     4861 atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc gatccatgtg
     4921 attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt ctcgcataaa
     4981 cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat tttttattaa
     5041 attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt tatcagtaag
     5101 aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca taagaaggcg
     5161 aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa acgaacgcgt
     5221 atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc atttgaacat
     5281 ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga acaacaaaaa
     5341 aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg aagaaagcga
     5401 tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc aggtatctca
     5461 ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc gacttcagaa
     5521 acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct tggaagtgat
     5581 acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt ggatggtcct
     5641 tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat cccaggggta
     5701 ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt gcgtcaaact
     5761 ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt caaatcgttt
     5821 gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga aaaagatctg
     5881 aatctggatg gtgtagatat taaaaaaggg ggccagaaga tggatggaca tgttcttttg
     5941 cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag acgccaacag
     6001 caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat ccgtgcacct
     6061 tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg gttcttgacg
     6121 aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag tgtgccagta
     6181 gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga aattgataat
     6241 caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa aagatcaaga
     6301 agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta aaaaacgctt
     6361 tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat ctgaacttca
     6421 aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact gaagcatcag
     6481 gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca gaagaagcaa
     6541 aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg tttgcagata
     6601 cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta gtcatccaga
     6661 caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt ggtaagattg
     6721 agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact gatcgatttg
     6781 gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc tggaaacatc
     6841 aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt cgactatacg
     6901 gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag tccgcaaaaa
     6961 attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac cttcacaagc
     7021 aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt atgaaatcag
     7081 cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt tggtagatat
     7141 ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa atttgcctat
     7201 tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat tttcgatcgt
     7261 aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg atcgagaagg
     7321 tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg aaaagaacta
     7381 tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt ttaagaatct
     7441 ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc agaacgctga
     7501 ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac gaaaagggat
     7561 tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga tttatcagct
     7621 gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag gtttaattac
     7681 gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta aaacccaaga
     7741 agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa acggacggat
     7801 tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt cgctatctag
     7861 tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac ttgatgctgc
     7921 tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct tttacattac
     7981 agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat ttttaggaat
     8041 agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg cgaaaaaagt
     8101 tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac gctttgcgaa
     8161 gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg tggctatgtt
     8221 tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc aattaaagct
     8281 aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac gcatcaagtc
     8341 aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag cagaagtaga
     8401 actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag gaacgttgat
     8461 cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag aaattctcaa
     8521 agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa atcttaagca
     8581 gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag gaatcactct
     8641 ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg cgcgctggga
     8701 agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt tagagaatat
     8761 caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca aggtagactt
     8821 ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa acgatcaatt
     8881 aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa tcgctacatg
     8941 cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga agctgactaa
     9001 aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca aaggttttac
     9061 tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga caatcggttt
     9121 ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca aagaaacatc
     9181 aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc ggaatattta
     9241 ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga atcaagcaaa
     9301 aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt gaaaaaagtg
     9361 cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta ccaagcattt
     9421 ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca aacagcatat
     9481 tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac ttattttttt
     9541 cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt ggtggtggta
     9601 gcggctatga tcattattta ttttctacca gttattacac aagtatttca tcaatatttt
     9661 ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc tttgtcattt
     9721 tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat acttggatat
     9781 aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa aaaaatgaaa
     9841 aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc agaagtaaaa
     9901 gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc tgatggttct
     9961 gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga ttcgatcatg
    10021 aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt tcgactaaag
    10081 aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa atgatcggtg
    10141 taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca gtattcaatc
    10201 aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat ttagctaaaa
    10261 aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg ttgtttcttc
    10321 aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt atcataatga
    10381 ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt tatttacgac
    10441 aaaccgcaat aaaatgaacg aattaaaaac tttatttctc aattagatca gcatgtcatt
    10501 aaggaatatg ctgatttttt tatgtttaat catacaaaat agttttttca taaaaatatg
    10561 gaaaatatgg attaattttg aaataattga tttaaaagga atacaaaaga gtgaatagta
    10621 ggaaatagtg gtttagaact agtgggctga ttttttatat attttagttt acataatata
    10681 tattatacaa agtagattta aaaaatgata aacctctctc gaacggtttt ttatcaaaaa
    10741 tcgtcatttt tccatcaaaa tctgcttatg caaaattata aaaaaatttt gcagtttttc
    10801 ttactttttt tgtcgtttaa ttgaatgata aaaaccagtt tctttattca gttaatcaaa
    10861 tatgaacgaa ttagatatga atcattgtat ttgccgttgc ggaaggattt tttaattaac
    10921 cacagataaa cattgataac gattcgctta ttgtaatcaa cagattaaat ttcatatgtt
    10981 catatgtacg gatacatttt cggaccgatt aattttaacc attctgttta tttgcttacc
    11041 tattagatat caataaaaaa ctgcttaaca gctatttcag ctgttaagca gttttaggat
    11101 ttttagttca tattcattgt ttcttataat acagtgttca atacttctct taaagcactt
    11161 gttgatttgc tgaacattgc tttttcttct tcgttgattg ataattcaac aacttcacga
    11221 acaccatttt cgtcaacgat tgatggcaca cctgtaaaga tatctttttc gtcgtattca
    11281 ccagttaaat aagcagatac aggcaataca gcttgttcgt tattcaagat agcttttacg
    11341 atacgtgcag tactcatacc aataccatag taagttgctt ttttacgatc aatgatctca
    11401 taagcagcat tacggacttt atcagcaatc acatctaatt catcttgggc aatacgatgg
    11461 tctttttcta caatttcaaa cactggttta ccaccgacag ttgtatgtga ccatgcagca
    11521 acttctgaat caccatgttc acctaagata tagccgtgga cgctgcgtgg gtcaactttt
    11581 aatttcaatg cgatttcttt acggaaacga gttgtatcca atgttgttcc agtaccgata
    11641 acacgtgatg ttggcagacc agattcgttc caagcgatat atgtcaaaat atctactggg
    11701 tttgaagcaa caacgataat accgtcgaat cctgatccca tgatttcttt tacgatttgg
    11761 cgcatgattg acgcatttgt tttaactaaa tctaaacgag attgaccagg tttttggttg
    11821 ataccagctg tgataacaac gatatcagca tctttacatt cttcatagcc acctgaccat
    11881 acagccacat tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc ttccccttct
    11941 gctttttctt gattcacatc gatcaatact aattcattag agattccttg gttgatcatt
    12001 gcataagcga tacttgttcc aacaaaacct gtaccgacga ttactacttt acgacttgtt
    12061 tttttcatga ataaacagct ccttttcata actatttgtt actttcatga aaagtatagt
    12121 cttgtttatt cattttatca acatgttaat tataatagtt gttattataa tttttgatgg
    12181 aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct atatcccctg
    12241 ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct gcaacgatat
    12301 aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg aaagattttc
    12361 ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct tgaataattt
    12421 gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca acgaaaaact
    12481 cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca gttcttgaaa
    12541 ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt agccaaaatg
    12601 agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat ctaatactct
    12661 aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt ctaataaatg
    12721 agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct tatgactgta
    12781 tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc
    12841 gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa
    12901 agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag ataaggtttc
    12961 ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga ttctttgagt
    13021 agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt ctccattaat
    13081 aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat gtgtatgctc
    13141 gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt gaaatagatc
    13201 ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat cattgatatt
    13261 cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta
    13321 attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg cttattagtt
    13381 cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact ttctatggtt
    13441 gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata ctatattttt
    13501 gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga tgaaattttc
    13561 tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg caattcccag
    13621 tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa cgactgctcc
    13681 ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga aaatcggaca
    13741 aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc tccccctctt
    13801 ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg gctttggatt
    13861 gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg aacgagcctc
    13921 tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga catttgttgc
    13981 aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca tcagcttacc
    14041 tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac ttcaatcgca
    14101 tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta gttatggtat
    14161 ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag ccacagtcct
    14221 gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt
    14281 ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac atcgctatat
    14341 gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca ctgtgataaa
    14401 cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt tattcattcc
    14461 ttacttattc aaccaagtaa gtatccaagc gcccaagtac gcggagagtt tcaatacttc
    14521 aatcttattg gttggcgcta atttaggtac tttcattgca ccatatggtt tgctctttct
    14581 ctcttttgct tctcttggga gtaaaacact tgctgttttc gtcaatggca gtatcttgct
    14641 gttgattagc gcgcttatta gccttctagt agtacacagg acgaatagaa agacagtgat
    14701 aagagtagat gaagaaataa aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg
    14761 taaaatgaca aatgaaaaac ataaagatag atggctttgg tatcctggag actttgagat
    14821 ccgccatgga ctgctacaaa atttccaacg ggaagaacga ggttttgact ggccagcgta
    14881 ctggtacatg gatgattgcc atcggaacgt caagtttaag agatattact ttcttgatca
    14941 accttccatg tttaaagtga cgatccaagg agttgggtat gtagaaatta atggacaaaa
    15001 acatccatgt ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt
    15061 aggacataca tcagggctac cagcgatgtt tatcgaggga gacgaagtaa aatcagatat
    15121 tggctggaca gcaagtaatt ttattgaaga atatccagct ggctggagtc cgctttatgt
    15181 cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac
    15241 tgaaacggag attggcggag gaatcttatt cgattttggg cgagcagtga atggctgtgt
    15301 agaactaata tctctggatg accatatcga atctatcact ctatgttatg gagagtccga
    15361 tgtagaagct atggatatcg attattgcta ctataagcaa ataggaatga caagaaaaat
    15421 gaaagctcgt aaacgcgctt ttcgctattt gtttatacca gaggtttcta ctggaatggt
    15481 tcaacttcaa gtttttcatg aatttttatc tctttcgaaa caagctgctt tttcaagcga
    15541 ttcttcttta ctgaatcaga tctggtcagt atcagaagaa gccttcaagt tatgtagtga
    15601 tttgtttttt atcgacggta tcaaacgaga tcgctggatt tggtctggag atgcgtatca
    15661 atcattcatg atcaatcaat atcttttttt tgacgaggaa atcaataaac gaacgatatt
    15721 agcattaaga ggacaaaatg aaatcaagca gcatatgaat actatcgttg actattcgat
    15781 cttgtggttg attggtatcg aaaaccacta tatgatgacg aaagactatg aatttttacg
    15841 catcgtctac cctaagatgc aatcattaat gagatacttg atggagcaaa cgaatgaatt
    15901 agggtttatt tatgggagag caaaagattg gatatttatt gattggtctg aaatggataa
    15961 agaaggaacg cttgctgcag aacaagtctt attactgaaa gcctatcatt ccatgatcac
    16021 ttgcggagat atcttagctg aaacatatgg agagaacgaa aattatcata ctcatctcta
    16081 tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac tacttttggg atgaagaact
    16141 tggcgctttt atcgattgct atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat
    16201 tttcgctgtt ttattcgatt ttgctaacaa agaccaacag caatcgatct taaagaatgt
    16261 cttattgaat gacactatca cacagattac tactccatat ttcaaattct atgaacaaga
    16321 tgctttatgc aaactcggac agacgaatat cgtctaccaa acgattcttg attattgggg
    16381 agggatgtta tctcatgatg cggtaacttt ctgggaagaa tacgacccta ctcaaacagg
    16441 aaatgaaaag tacagtatgt atggagatcc ttatggaaaa agcttatgcc atgcatgggg
    16501 agcaagccca atctatcttt taggacgcta ttttatcggt ttgtatccaa caaagccagg
    16561 ttatgaagaa tttatgatac aacctaatct agattcattt gaaacactga attgtacgct
    16621 acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa gataagatcg tgattacttc
    16681 tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca
    16741 gccacttgtc ttaacaagct attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac
    16801 atatcagaag actttaaatc ttcttctgat attgtccaag caatttttat tttgaagttt
    16861 gcttaattct gttctttaga aacaatgttt gttctttttc cggcaaaaag aaagctagat
    16921 aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca aggctatttg accgtattct
    16981 cctagtttcc caagtacaag tcctggtact agataatcag tatcggcaaa tgtagaagaa
    17041 gcgattttaa gatctcctaa tactggaagg ataaataatg gcagccaact aatcaataag
    17101 ctattgaaag cagaacctaa taccgcccct cttctaccgc cagtcgagtt gccgaagaca
    17161 ccagaagcag ctccgcagaa gaaatgtcct actaccccag gaatgataac tgtagtttgc
    17221 aagcctatca tagcaaacat ggatagtgtt cctacaaaga agctaacaaa aaagccaatt
    17281 aatacagcat taggtgcata tggaaaaacg ataggaacat ctaatgctgg tttagaatcg
    17341 ggtacgagtt ttttagcaat cccttggaat gctggaacga tttctgccaa caccattcga
    17401 accccttgaa ggacgataac aaacccagct gtgaatgttc cagcttggat cagtgcaaag
    17461 attacagcgt tgtctccatt gcttagttct ctttcaacaa atccaggacc agctaacaaa
    17521 gcaagtgcga catatacgat cgacatcaag agcatgatcg atacagtaga atctcgcaaa
    17581 aagctgaaac ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac
    17641 tttccgatcc atccactaga agcataaccg atatttccgg tatgccctaa cgcaacttga
    17701 ttgtttccag tgatgttctt cataaacggt tgacaaagag caggcgtaat agttaatagt
    17761 gttccttcga agaccccgcc taataagatc atccacaaat ttgcagtaaa ccccgcgctt
    17821 actaaaatga ctgctgttag acaagaaaca taaagcattg cttgacctgt aagaaaaatg
    17881 aatttatatc ttgtgaatct agcaagtaaa atattcacga tcatccctac tagcatgatc
    17941 aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc attgctagga
    18001 actactcctt gagtatgcag agctgtttgg aacatcgaag caaaaggggt tagtgaatta
    18061 ctgattaaac cagcaccgcc gatcagtact aggaatccga caaatgtttt gatagttcct
    18121 tggatcactt ctgaaaattt cttcttctga agaacgagac caagcagtgc aatcaaagaa
    18181 acgaggatgg agggctgact gacgattgac acaaacaaat tcaacaattt atccattgat
    18241 agttcctccc ttgaatcatt tttattcttt tgacaagatc ttcgttactt tttcttttac
    18301 ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta tagaagttag
    18361 cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag atacacttcc
    18421 aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg caagtgcttg
    18481 tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata ctgcaaaaat
    18541 tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt gttgatgcgt
    18601 gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt gccaaagccc
    18661 gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg ggatgggctg
    18721 gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct acttcgttta
    18781 cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac
    18841 catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt ttattttctg
    18901 ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt tgatccgata
    18961 attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc tccttctttt
    19021 ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg tctacgcctt
    19081 cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa ggcattgaaa
    19141 atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt tccaagtcag
    19201 tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg agaggtacag
    19261 tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca gctgaattag
    19321 tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag atcaatgaag
    19381 agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt tcttctgcgt
    19441 ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct tcttcaggga
    19501 tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg
    19561 aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa gaataggaaa
    19621 tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca cgaaacgaag
    19681 caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa cattccagca
    19741 aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag caaagagaga
    19801 aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat acttgtcgac
    19861 taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt tccatggatg
    19921 tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc attcgatttg
    19981 gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta actttggcgt
    20041 aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca gcaagtgact
    20101 gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct gaccctagca
    20161 gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct aattcatttc
    20221 ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat
    20281 gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct
    20341 cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct gcccagtcga
    20401 ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca aaaggagtta
    20461 attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt tctagactga
    20521 tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg ttcacctcct
    20581 acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca acttacagtt
    20641 ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg
    20701 tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg atcatgttta
    20761 ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt ttcgatatct
    20821 ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt tgaaccagat
    20881 aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga ttcttctttg
    20941 tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt tttaggggcc
    21001 attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct
    21061 agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa aacaaacccg
    21121 attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga ttccttcttc
    21181 caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg cgccattaac
    21241 taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg aaattacttt
    21301 ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat gttcgggcaa
    21361 atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg
    21421 atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac ttggacgttc
    21481 tttgatgcgg aaataactgg attttatcga aattttcata tttccataga aaaaatccag
    21541 atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat agctgatatc
    21601 atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat gtccatataa
    21661 atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc tgaaataatc
    21721 ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta ctttttggac
    21781 tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata gacctttttc
    21841 ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag gtttctctac
    21901 tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa attgactagg
    21961 tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca gctgatcagt
    22021 atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa tttttttgat
    22081 attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg tactctttcc
    22141 attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat gttttcagta
    22201 tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa gttcagaaaa
    22261 acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa
    22321 gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag atcatgctta
    22381 ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg attgtcgatt
    22441 acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta gcaaaagcta
    22501 cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc agcggattta
    22561 aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa
    22621 cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa
    22681 tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa catgaagatt
    22741 tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg
    22801 attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat aagtttttga
    22861 tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact agcgaggatt
    22921 gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata atgacctatt
    22981 tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca gaaatggcca
    23041 aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct aaagtagcga
    23101 ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca gtgatatatt
    23161 ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg atgcaaaaag
    23221 gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa taagaaagat
    23281 agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt tctttttcaa
    23341 acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa tgaggaatag
    23401 ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa ggaggaaaat
    23461 cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg atactttctg
    23521 tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga caagtaagcg
    23581 atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct gccctttttc
    23641 gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga cttgataagc
    23701 gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt tcggtaagtc
    23761 tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat gtgtattttc
    23821 tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta gcagcacagt
    23881 attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca aaataccgat
    23941 cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct tagtaaaggg
    24001 atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg tcatgttttc
    24061 ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat aatgagagat
    24121 gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag ctagtatccc
    24181 tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt tctttggttt
    24241 catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc atttgcgtaa
    24301 tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag
    24361 caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac acatagattc
    24421 ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt tcaggagaaa
    24481 tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca cggtttgtta
    24541 atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca tattttagac
    24601 ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg
    24661 atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct gtgtcactat
    24721 cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta acgtcaccat
    24781 ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt gtttgatcat
    24841 ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga tctgcttttt
    24901 cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat gaaggaatat
    24961 aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa taatttactg
    25021 ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa gaatcattat
    25081 caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg
    25141 ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat tgaatttttt
    25201 caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact ttagacgtat
    25261 catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca aaaggcgtga
    25321 ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct aaattatatt
    25381 ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc tcggcaaagt
    25441 cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg gtttgatcag
    25501 ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg cttggtgttc
    25561 cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg gcagtaccga
    25621 tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag agatggatag
    25681 tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc atctcatata
    25741 cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt tcttctttga
    25801 tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg acatttccat
    25861 cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg actgcttgtt
    25921 ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga gtgacgttat
    25981 aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc atttctttcc
    26041 cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga aggatgactt
    26101 gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg agtaatggta
    26161 aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc attttttcac
    26221 tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca taccaacaac
    26281 gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc tattttcttt
    26341 tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa aagttgaaat
    26401 ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga tctcttttaa
    26461 tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat cggaaatcag
    26521 gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt gctggaggta
    26581 atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat ctggtttatt
    26641 tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct ttgtactgac
    26701 accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact
    26761 gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt ccaacgaagg
    26821 gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc cttctgtcag
    26881 cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg cttcatccgc
    26941 actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg tcgtatgcaa
    27001 ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta ataccttctc
    27061 aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga tagttgaaag
    27121 tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg ccttttcttt
    27181 gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt tttcttgatc
    27241 tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg tagttgttgc
    27301 ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt gtgcttcttc
    27361 taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat aaacttggaa
    27421 tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc cgtcatttgg
    27481 atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat
    27541 ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta acaagaagag
    27601 acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc ttttttcaaa
    27661 aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat tcctaaacgg
    27721 ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc taaagaaatc
    27781 tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc cacttgaatt
    27841 gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta tccttctggt
    27901 gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag aaaggtatct
    27961 gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag tctgaatttt
    28021 gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc aaacgctttt
    28081 agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt ttttcccgtg
    28141 taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga agtatcatat
    28201 ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga atcaaaactg
    28261 gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc atttgcttct
    28321 tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt cccttgcatc
    28381 gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt caactggatc
    28441 gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc agaagcgatc
    28501 ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt tgtttctgtc
    28561 agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata ggttccgtcg
    28621 ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac agcgccaata
    28681 agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg tgtgaattgt
    28741 ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg tacaggtaat
    28801 tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg tagtgataat
    28861 atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc acgttcccac
    28921 gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt ccaagctgca
    28981 gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg atcagctaga
    29041 ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg agctttagca
    29101 gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa agttgtcctg
    29161 ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg gattctcact
    29221 tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct cttgatggta
    29281 tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac actcttgact
    29341 gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat ttgtccattg
    29401 cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat atctgttgca
    29461 tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat ttgtcgcata
    29521 cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag ttggatgcca
    29581 agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat ggttgcgata
    29641 aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata gattcttgag
    29701 attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc gtaataattg
    29761 ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac accatccgtc
    29821 agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc tgcatctctt
    29881 agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga ttttacttga
    29941 tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct atatccttcg
    30001 cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc tgctaaagta
    30061 tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc gttcatactt
    30121 cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg tacatttcca
    30181 cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc atactttcgt
    30241 atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg ggtccgatca
    30301 ttaggatctc cattccaaga atttatacca tcctattgat ttgttccgtt tttattgcct
    30361 tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg ataagtgccc
    30421 tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact cacagctggc
    30481 aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt tgatgattct
    30541 tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt ttctgtctca
    30601 tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct gtggtaaagt
    30661 gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga gatttccaac
    30721 gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct ttccacatca
    30781 cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact ggtgttattc
    30841 gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac ttcagctgaa
    30901 attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa tggcacaaag
    30961 tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc tgtggtcgtc
    31021 atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa ttattaaaat
    31081 aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg aagggcgcta
    31141 aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga ttccttcgat
    31201 taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat caggttgttg
    31261 gaacgaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg
    31321 atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt tgtaaggtat
    31381 atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc acaaaaattg
    31441 gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat ggaaatgtcg
    31501 caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg taaaacgatt
    31561 gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa
    31621 tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg ttttcagtta
    31681 actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt tttaagttac
    31741 tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga aattatggtc
    31801 aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca atctttcttt
    31861 attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt tgaatggaaa
    31921 gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt aatcttgaaa
    31981 aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta ttttgtcaat
    32041 acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa
    32101 aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga agaatatgtt
    32161 gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga tacgactcat
    32221 ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat acgtctatat
    32281 gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa tctgatttat
    32341 tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca ccattatact
    32401 tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca atatattgaa
    32461 gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac tgctattact
    32521 gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac gaatcaaccc
    32581 attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt cgcaaatgaa
    32641 gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg tataaaccac
    32701 cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta ttccttcagc
    32761 aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc aattatttgc
    32821 aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga aactaactgc
    32881 ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc atttttcgtt
    32941 cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat ttcgctgggt
    33001 taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc accactctaa
    33061 atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt ttaagatatc
    33121 ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt ccaaatcttg
    33181 aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt ctgagacttt
    33241 gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga ttctctgttt
    33301 ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc ctttatctct
    33361 gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt ttctcttctt
    33421 ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc atacggacat
    33481 catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact agtaatttga
    33541 cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct aagatcaagc
    33601 ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca ccagaaaggt
    33661 ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag gaatcgattt
    33721 ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt tcctttaccg
    33781 ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta aagcgtaaat
    33841 ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact atttctccgg
    33901 aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct gaaccagaag
    33961 gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct ttcaaagctg
    34021 agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt aatatttttt
    34081 tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg tacgtatcga
    34141 aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca accattgtga
    34201 ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa
    34261 aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta aagatttagc
    34321 gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag tcttctgaag
    34381 tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca aaaagtatat
    34441 catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa tgaatgtccc
    34501 aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag ctgaatcctt
    34561 tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt gtccatactt
    34621 caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt aataagtttc
    34681 aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc
    34741 aattttatag cctgaatcag ccagattttg agagatgatg atttcattct ttgttttaaa
    34801 ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt
    34861 aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg gtgctttctc
    34921 tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa atacttgatt
    34981 tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt ttttaaactc
    35041 ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa tcaagaacat
    35101 aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga tagctagaaa
    35161 catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg ttgaatagtg
    35221 taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata aattgtctaa
    35281 gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc cttatttatg
    35341 ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt aaactattag
    35401 tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct gcaatcggcg
    35461 agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt ggagtttatg
    35521 atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa
    35581 tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat agtttggaag
    35641 ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct ttctatcgag
    35701 aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc tttgatgaaa
    35761 atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc ttgtataaag
    35821 aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt ttacaaaata
    35881 agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt gattctgata
    35941 cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg cgaagtactg
    36001 gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa agggtgtgat
    36061 gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca
    36121 gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt aaaccaatct
    36181 gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg aacagtacaa
    36241 ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca aacggaacaa
    36301 aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt taacactcaa
    36361 gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg tgggcgtttc
    36421 aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca aaatattgaa
    36481 ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa aaaagcggag
    36541 acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca aatcaagaat
    36601 tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg gattctctct
    36661 attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct ttcttggaga
    36721 agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt aacgattatt
    36781 caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga agaatccgaa
    36841 agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc ggatttgctg
    36901 acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat caatacacaa
    36961 gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga tggaaaagag
    37021 tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa aatccatcaa
    37081 gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga taagatcatt
    37141 ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac tggcccttct
    37201 atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga tcaatctcgg
    37261 gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt aatcgaacag
    37321 catcatggac agatttctgt atccaattat cttccacaag gagtcgattt taaagtccaa
    37381 ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga caaaaatctt
    37441 gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct tcggaaataa
    37501 gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac gcttacgatt
    37561 ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat tcttctcggc
    37621 taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc ccaacttctt
    37681 cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg atagtaatcg
    37741 tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact ggtttgcgtt
    37801 cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg ttgaccacat
    37861 gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct aatttttctt
    37921 cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct tcagtcttgc
    37981 ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct ggtgtcatat
    38041 tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc
    38101 cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta tcttgtgtat
    38161 ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg acaaattttc
    38221 catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta gccgtctcag
    38281 aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa cggtcttctt
    38341 caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg tcaaatccta
    38401 gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca tacccttcgt
    38461 tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt ccgatgaccg
    38521 ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat tggtatattg
    38581 ttacttataa atttattcta taggagacga ttgttttcta caaatattta gattggaatt
    38641 tgattaaaac aatttaattg aaatttgaaa acatttatgt taatgaaagt catattctaa
    38701 taaaatgtca tttgtttcta tatgaatttt tatgaaaaat ttcatataat ttccactcgt
    38761 tgtgatggtt taatttatcc aatccatcaa taagcaaaca acttatataa aaaaggatta
    38821 attctcaata taattgctcc taccatagaa taaatcttca tgtaaagtaa tgaagtacca
    38881 actatttcta atacttcttt aagaattatc ccaacaatat cgttagacaa accattattg
    38941 aataaatgga gagccaatcg attctgaaaa aatgttttct aatttttcgg aaataaaaga
    39001 aagagcctcc tgccattaga acgaaccaaa tccatttcat tgctcatgaa gtaaccacat
    39061 agctaaaaag aggataaatc aattaaaaga tcaaaagaat gactgcacta ataaaataac
    39121 taaaaatgta tcttcttact tcatatcttt tagctagtac ttagttaaaa atttgcttcc
    39181 taagatcaag acaaactgtc tatcttcatt tgatgaaatg cggtaaaata tagaaaatga
    39241 acactattgg agggaatttg taatggacag caaaactaga aaacaactgc aagaaaagtt
    39301 aaagcaacgt acacacttgc tcccttccgt ctttcaacag tttttagaag aaaatcgttc
    39361 ttccctttca ttaggcagtc gctatgaata cgcaaaagat tttcatctgt ttactgatta
    39421 tttgagaaaa gtattttctg aagaagtgga tgatcagctg attatccatt tagaaaaaca
    39481 agattatcag aatttcttag ctaaaatcta tcgacatact cgttcgttcc aaactacagc
    39541 gaaccaagaa acggagcaat tgtttgaaaa tagctattac gggatttcaa ggaagcaata
    39601 ttcattaaat cattttttac gatttttgta tcaaaaagga ctgattgaag aagagatttc
    39661 tcttttgggt acaagtttac cggaaacaac caaagcagct ccgcgatata tcgatgcaga
    39721 acttttcaaa gattttgagc atttgtttat cccgattgaa ggctttcaaa caagcagaga
    39781 agctagcttt gaagaaaaga atagaccgag gaaccttgca attactgcca ttcttctgta
    39841 ttgcggatta aagatccatg aagtagtaga acttaaacgt aaagatgtat ctttgaccaa
    39901 gcagattctt aatctgaata gaaaagaaaa cagactgaat aaagtaccaa taccaaaaga
    39961 agcgatgcca tttctggaga actattttaa gttaacagca caaaatacgg acgaaaattc
    40021 ttttgttttt cgttcctcac atgatcagca aatttctcct cggactattc gccaaatttt
    40081 gagcaaaatt acagtatata aaaacgtatc gccagaacta tgcagaaaaa catatcgtta
    40141 ttatgcagaa ctatatttag atgatgctga cgcggtaaat tatttatgtg gtaatcgata
    40201 cctctcatcc cattcttttc aagatatctg ggaaaagatg gttgattttt cttatcacga
    40261 attagcggca ttcgttcggg tttcttccat ataaaacaaa tgtatgatct ttttttcaca
    40321 ataaactatg ttattatata aaggattatg ttacgattat cttgaatttg tatattactt
    40381 aacaaaagat aatggagtgt gaatgatgga gaccataacg tattcccctt tgaacttatt
    40441 tacgaatttt caagaagctg cagctaactt ttccgagacc ccgattatct ttgatgagcc
    40501 actttcagct ttcccagaat taggacttga aacaacttac aaaaaatgct gcgaaagtgt
    40561 gttgaaacgg gcttatcaat tggctcatct tggagtgaaa tccggagaca aagttattat
    40621 ttataagagt tccgcttttg acacatacct tctagctgtt tctgtgtctt accttggtgc
    40681 agtgcctgtc atgacttctt atcatctacc aacaacaaca atggaagtat ttatcgatcg
    40741 tttagaagac cctttcattt tatttgatga tgagacaaaa aagcgagtga gagaaatttc
    40801 aaatggaaca aaatcaaaac agattcc
//
LOCUS       DAFEZB010000018        39890 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFEZB010000018
VERSION     DAFEZB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.018"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(28..726)
                     /locus_tag="LP859_000999"
     CDS             complement(28..726)
                     /locus_tag="LP859_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL3028969.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="LP859_001000"
     CDS             complement(719..2071)
                     /locus_tag="LP859_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL3028970.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="LP859_001001"
     CDS             complement(2294..3694)
                     /locus_tag="LP859_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL3028971.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="LP859_001002"
     CDS             3855..4592
                     /locus_tag="LP859_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL3028972.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="LP859_001003"
     CDS             4717..5196
                     /locus_tag="LP859_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL3028973.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="LP859_001004"
     CDS             5193..5960
                     /locus_tag="LP859_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028974.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="LP859_001005"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="LP859_001005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL3028975.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="LP859_001006"
     CDS             complement(6863..7465)
                     /locus_tag="LP859_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3028976.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="LP859_001007"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="LP859_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="LP859_001008"
     CDS             8146..8730
                     /locus_tag="LP859_001008"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL3028977.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="LP859_001009"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="LP859_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL3028978.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="LP859_001010"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="LP859_001010"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL3028979.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="LP859_001011"
     CDS             10631..10810
                     /locus_tag="LP859_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028980.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="LP859_001012"
     CDS             10832..11857
                     /locus_tag="LP859_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL3028981.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="LP859_001013"
     CDS             12032..13276
                     /locus_tag="LP859_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL3028982.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="LP859_001014"
     CDS             13429..14376
                     /locus_tag="LP859_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3028983.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="LP859_001015"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="LP859_001015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL3028984.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="LP859_001016"
     CDS             complement(15278..15520)
                     /locus_tag="LP859_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3028985.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="LP859_001017"
     tRNA            15641..15713
                     /locus_tag="LP859_001017"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="LP859_001018"
     CDS             complement(15832..16470)
                     /locus_tag="LP859_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL3028986.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="LP859_001019"
     CDS             complement(16593..17225)
                     /locus_tag="LP859_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL3028987.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="LP859_001020"
     CDS             17576..17875
                     /locus_tag="LP859_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL3028988.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..20535
                     /gene="acm"
                     /locus_tag="LP859_001021"
     CDS             18370..20535
                     /gene="acm"
                     /locus_tag="LP859_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL3028989.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20807..21292)
                     /locus_tag="LP859_001022"
     CDS             complement(20807..21292)
                     /locus_tag="LP859_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL3028990.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21382..21727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21772..22413
                     /locus_tag="LP859_001023"
     CDS             21772..22413
                     /locus_tag="LP859_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3028991.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22432..23067
                     /locus_tag="LP859_001024"
     CDS             22432..23067
                     /locus_tag="LP859_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3028992.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23085..23900
                     /locus_tag="LP859_001025"
     CDS             23085..23900
                     /locus_tag="LP859_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028993.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24219..25655
                     /locus_tag="LP859_001026"
     CDS             24219..25655
                     /locus_tag="LP859_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3028994.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25844..28291
                     /locus_tag="LP859_001027"
     CDS             25844..28291
                     /locus_tag="LP859_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL3028995.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28521..29588
                     /gene="rlmN"
                     /locus_tag="LP859_001028"
     CDS             28521..29588
                     /gene="rlmN"
                     /locus_tag="LP859_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL3028996.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29781..30884)
                     /locus_tag="LP859_001029"
     CDS             complement(29781..30884)
                     /locus_tag="LP859_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3028997.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31437..31985
                     /gene="aac(6')"
                     /locus_tag="LP859_001030"
     CDS             31437..31985
                     /gene="aac(6')"
                     /locus_tag="LP859_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL3028998.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32531..34192
                     /locus_tag="LP859_001031"
     CDS             32531..34192
                     /locus_tag="LP859_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3028999.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34262..34609)
                     /locus_tag="LP859_001032"
     CDS             complement(34262..34609)
                     /locus_tag="LP859_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL3029000.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34693..35634
                     /locus_tag="LP859_001033"
     CDS             34693..35634
                     /locus_tag="LP859_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029001.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35635..36678
                     /locus_tag="LP859_001034"
     CDS             35635..36678
                     /locus_tag="LP859_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029002.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36684..37754
                     /locus_tag="LP859_001035"
     CDS             36684..37754
                     /locus_tag="LP859_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029003.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37744..38697
                     /locus_tag="LP859_001036"
     CDS             37744..38697
                     /locus_tag="LP859_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3029004.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38709..39719
                     /locus_tag="LP859_001037"
     CDS             38709..39719
                     /locus_tag="LP859_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3029005.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
BASE COUNT        13605 a         6869 c         8129 g        11287 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
    19681 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
    19741 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
    19801 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
    19861 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
    19921 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
    19981 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
    20041 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
    20101 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
    20161 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
    20221 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
    20281 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
    20341 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
    20401 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
    20461 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
    20521 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
    20581 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
    20641 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
    20701 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
    20761 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
    20821 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
    20881 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
    20941 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
    21001 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
    21061 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
    21121 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
    21181 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
    21241 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
    21301 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
    21361 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
    21421 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
    21481 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
    21541 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
    21601 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
    21661 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
    21721 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
    21781 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
    21841 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
    21901 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
    21961 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
    22021 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
    22081 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
    22141 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
    22201 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
    22261 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
    22321 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
    22381 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
    22441 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
    22501 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
    22561 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
    22621 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
    22681 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
    22741 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
    22801 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
    22861 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
    22921 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
    22981 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
    23041 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
    23101 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
    23161 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
    23221 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
    23281 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
    23341 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
    23401 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
    23461 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
    23521 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
    23581 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
    23641 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
    23701 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
    23761 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
    23821 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
    23881 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
    23941 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
    24001 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
    24061 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    24121 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
    24181 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
    24241 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
    24301 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
    24361 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
    24421 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
    24481 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
    24541 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
    24601 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
    24661 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
    24721 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
    24781 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
    24841 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
    24901 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
    24961 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
    25021 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
    25081 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
    25141 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
    25201 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
    25261 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
    25321 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
    25381 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
    25441 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
    25501 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
    25561 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
    25621 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
    25681 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
    25741 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
    25801 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
    25861 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
    25921 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
    25981 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
    26041 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
    26101 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
    26161 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
    26221 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
    26281 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
    26341 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
    26401 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
    26461 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
    26521 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
    26581 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
    26641 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
    26701 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
    26761 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
    26821 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
    26881 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
    26941 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
    27001 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
    27061 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
    27121 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
    27181 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
    27241 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
    27301 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
    27361 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
    27421 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
    27481 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
    27541 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
    27601 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
    27661 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
    27721 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
    27781 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
    27841 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
    27901 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
    27961 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
    28021 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
    28081 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
    28141 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
    28201 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
    28261 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
    28321 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
    28381 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
    28441 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
    28501 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
    28561 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
    28621 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
    28681 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
    28741 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
    28801 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
    28861 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
    28921 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
    28981 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
    29041 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
    29101 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
    29161 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
    29221 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
    29281 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
    29341 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
    29401 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
    29461 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
    29521 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
    29581 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
    29641 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
    29701 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
    29761 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
    29821 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
    29881 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
    29941 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
    30001 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
    30061 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
    30121 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
    30181 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
    30241 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
    30301 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
    30361 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
    30421 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
    30481 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
    30541 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    30601 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    30661 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    30721 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    30781 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    30841 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    30901 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    30961 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    31021 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    31081 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    31141 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    31201 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    31261 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
    31321 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
    31381 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
    31441 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
    31501 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
    31561 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
    31621 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
    31681 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
    31741 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
    31801 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
    31861 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
    31921 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
    31981 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
    32041 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
    32101 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg
    32161 tgaaaatggc tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    32221 aacaccgttt attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt
    32281 attctttttt atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt
    32341 ttggaagacc atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct
    32401 cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta
    32461 ttcattaaac tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg
    32521 ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact
    32581 tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc
    32641 agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc
    32701 agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg
    32761 tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga
    32821 agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc
    32881 tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc
    32941 tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa
    33001 agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac
    33061 gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca
    33121 atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa
    33181 tggaccattc acattagaag attttgatgg accaggaaca gatacagaat ggacttataa
    33241 gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt
    33301 agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat
    33361 cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga
    33421 agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt
    33481 gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact
    33541 aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac
    33601 aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc
    33661 aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt
    33721 gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca
    33781 agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga
    33841 ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga
    33901 cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag
    33961 caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca
    34021 agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt
    34081 tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca
    34141 ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat
    34201 accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca
    34261 cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa
    34321 tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt
    34381 ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac
    34441 ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata
    34501 gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa
    34561 ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc
    34621 tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag
    34681 ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc
    34741 atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg
    34801 ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc
    34861 ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga
    34921 gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga
    34981 atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg
    35041 attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc
    35101 gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc
    35161 tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc
    35221 ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg
    35281 gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc
    35341 cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta
    35401 ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct
    35461 attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc
    35521 atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta
    35581 ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa
    35641 atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta
    35701 caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa
    35761 gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg
    35821 gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa
    35881 aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat
    35941 ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca
    36001 aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt
    36061 atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt
    36121 ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa
    36181 cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc
    36241 gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca
    36301 atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct
    36361 ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc
    36421 tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc
    36481 tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg
    36541 agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact
    36601 ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat
    36661 cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct
    36721 cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata
    36781 aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa
    36841 gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag
    36901 gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag
    36961 cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag
    37021 tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat
    37081 tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc
    37141 atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc
    37201 cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga
    37261 ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc
    37321 atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa
    37381 tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg
    37441 gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg
    37501 gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat
    37561 tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca
    37621 aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc
    37681 aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg
    37741 ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa
    37801 aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc
    37861 ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg
    37921 aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat
    37981 aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct
    38041 tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga
    38101 ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga
    38161 ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt
    38221 attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt
    38281 tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa
    38341 gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa acatattagt
    38401 gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc
    38461 tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat
    38521 ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa
    38581 gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc
    38641 ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag
    38701 ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc
    38761 aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc
    38821 agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa
    38881 aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt
    38941 tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga
    39001 aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa
    39061 atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag
    39121 ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg
    39181 cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg
    39241 cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat
    39301 ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct
    39361 ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa
    39421 aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat
    39481 ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg
    39541 cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt
    39601 cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt
    39661 ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa
    39721 aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac
    39781 ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa
    39841 acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt
//
LOCUS       DAFEZB010000019        39074 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFEZB010000019
VERSION     DAFEZB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.019"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LP859_001038"
     CDS             6..305
                     /locus_tag="LP859_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029006.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LP859_001039"
     CDS             378..1739
                     /locus_tag="LP859_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL3029007.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LP859_001040"
     CDS             1761..2021
                     /locus_tag="LP859_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029008.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LP859_001041"
     CDS             2035..2787
                     /locus_tag="LP859_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL3029009.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LP859_001042"
     CDS             complement(2881..3486)
                     /locus_tag="LP859_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3029010.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LP859_001043"
     CDS             complement(3539..4513)
                     /locus_tag="LP859_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL3029011.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGTSIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LP859_001044"
     CDS             complement(4891..5610)
                     /locus_tag="LP859_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3029012.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP859_001045"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP859_001045"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL3029013.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LP859_001046"
     CDS             7161..8558
                     /locus_tag="LP859_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3029014.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LP859_001047"
     CDS             8639..9079
                     /locus_tag="LP859_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3029015.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LP859_001048"
     CDS             9227..9553
                     /locus_tag="LP859_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3029016.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LP859_001049"
     CDS             9554..10303
                     /locus_tag="LP859_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029017.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LP859_001050"
     CDS             complement(10383..10838)
                     /locus_tag="LP859_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL3029018.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LP859_001051"
     CDS             complement(11265..11540)
                     /locus_tag="LP859_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029019.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LP859_001052"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LP859_001052"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL3029020.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP859_001053"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP859_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL3029021.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LP859_001054"
     CDS             14770..17436
                     /locus_tag="LP859_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3029022.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LP859_001055"
     CDS             17800..18552
                     /locus_tag="LP859_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3029023.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LP859_001056"
     CDS             complement(18574..19728)
                     /locus_tag="LP859_001056"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL3029024.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LP859_001057"
     CDS             19913..22354
                     /locus_tag="LP859_001057"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL3029025.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="LP859_001058"
     CDS             complement(22684..23388)
                     /locus_tag="LP859_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029026.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="LP859_001059"
     CDS             complement(23575..23820)
                     /locus_tag="LP859_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029027.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="LP859_001060"
     CDS             complement(23837..24688)
                     /locus_tag="LP859_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3029028.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="LP859_001061"
     CDS             24854..25858
                     /locus_tag="LP859_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL3029029.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="LP859_001062"
     CDS             complement(26099..26455)
                     /locus_tag="LP859_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3029030.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="LP859_001063"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="LP859_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL3029031.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LP859_001064"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LP859_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL3029032.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="LP859_001065"
     CDS             complement(30815..31576)
                     /locus_tag="LP859_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3029033.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="LP859_001066"
     CDS             31854..32375
                     /locus_tag="LP859_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL3029034.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="LP859_001067"
     CDS             32434..33294
                     /locus_tag="LP859_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL3029035.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33479..35794
                     /locus_tag="LP859_001068"
     CDS             33479..35794
                     /locus_tag="LP859_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3029036.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="LP859_001069"
     CDS             35976..37817
                     /locus_tag="LP859_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL3029037.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="LP859_001070"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="LP859_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL3029038.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12571 a         6875 c         8095 g        11533 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgtacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta tttt
//
LOCUS       DAFEZB010000020        38600 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFEZB010000020
VERSION     DAFEZB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.020"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(99..1229)
                     /locus_tag="LP859_001071"
     CDS             complement(99..1229)
                     /locus_tag="LP859_001071"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL3029039.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LP859_001072"
     CDS             complement(1878..3080)
                     /locus_tag="LP859_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3029040.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LP859_001073"
     CDS             complement(3140..3343)
                     /locus_tag="LP859_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL3029041.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LP859_001074"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LP859_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LP859_001075"
     CDS             4880..5692
                     /locus_tag="LP859_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029042.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="LP859_001076"
     CDS             complement(6716..6868)
                     /locus_tag="LP859_001076"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL3029043.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="LP859_001077"
     CDS             complement(7059..7937)
                     /locus_tag="LP859_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3029044.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="LP859_001078"
     CDS             complement(7976..9094)
                     /locus_tag="LP859_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL3029045.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="LP859_001079"
     CDS             complement(9091..11190)
                     /locus_tag="LP859_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL3029046.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="LP859_001080"
     CDS             complement(11241..12062)
                     /locus_tag="LP859_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3029047.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="LP859_001081"
     CDS             complement(12059..12868)
                     /locus_tag="LP859_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3029048.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="LP859_001082"
     CDS             complement(12889..13383)
                     /locus_tag="LP859_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029049.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="LP859_001083"
     CDS             complement(13395..13883)
                     /locus_tag="LP859_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3029050.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="LP859_001084"
     CDS             complement(13960..16656)
                     /locus_tag="LP859_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3029051.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="LP859_001085"
     CDS             complement(17084..18157)
                     /locus_tag="LP859_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3029052.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="LP859_001086"
     CDS             complement(18154..18981)
                     /locus_tag="LP859_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029053.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="LP859_001087"
     CDS             complement(18982..19788)
                     /locus_tag="LP859_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029054.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="LP859_001088"
     CDS             complement(19800..20885)
                     /locus_tag="LP859_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029055.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="LP859_001089"
     CDS             complement(20902..21444)
                     /locus_tag="LP859_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3029056.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="LP859_001090"
     CDS             21702..22691
                     /locus_tag="LP859_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL3029057.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="LP859_001091"
     CDS             22688..23425
                     /locus_tag="LP859_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL3029058.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="LP859_001092"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="LP859_001092"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL3029059.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="LP859_001093"
     CDS             23989..24621
                     /locus_tag="LP859_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3029060.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="LP859_001094"
     CDS             24706..25353
                     /locus_tag="LP859_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3029061.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="LP859_001095"
     CDS             25358..26341
                     /locus_tag="LP859_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL3029062.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="LP859_001096"
     CDS             complement(26744..28258)
                     /locus_tag="LP859_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL3029063.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="LP859_001097"
     CDS             28396..29202
                     /locus_tag="LP859_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029064.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="LP859_001098"
     CDS             complement(29377..29946)
                     /locus_tag="LP859_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029065.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="LP859_001099"
     CDS             complement(30029..32089)
                     /locus_tag="LP859_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL3029066.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="LP859_001100"
     CDS             complement(32366..33844)
                     /locus_tag="LP859_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029067.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LP859_001101"
     CDS             complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LP859_001101"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL3029068.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35150..36070)
                     /locus_tag="LP859_001102"
     CDS             complement(35150..36070)
                     /locus_tag="LP859_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3029069.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36183..36935
                     /gene="xth"
                     /locus_tag="LP859_001103"
     CDS             36183..36935
                     /gene="xth"
                     /locus_tag="LP859_001103"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL3029070.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37799..38332
                     /locus_tag="LP859_001104"
     CDS             37799..38332
                     /locus_tag="LP859_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL3029071.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT        11576 a         7960 c         6543 g        12521 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
    34141 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
    34201 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
    34261 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
    34321 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
    34381 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
    34441 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
    34501 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
    34561 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
    34621 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
    34681 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
    34741 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
    34801 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
    34861 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
    34921 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
    34981 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
    35041 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
    35101 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
    35161 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
    35221 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
    35281 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
    35341 ttccatcagc atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
    35401 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
    35461 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
    35521 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
    35581 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
    35641 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
    35701 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
    35761 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
    35821 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
    35881 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
    35941 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
    36001 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
    36061 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
    36121 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
    36181 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
    36241 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
    36301 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
    36361 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
    36421 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
    36481 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
    36541 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
    36601 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
    36661 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
    36721 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
    36781 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
    36841 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
    36901 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
    36961 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
    37021 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
    37081 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
    37141 ttattaccgc agatacaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca
    37201 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
    37261 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg tgaaaaagct
    37321 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
    37381 ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc
    37441 agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat
    37501 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa
    37561 aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca
    37621 taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg
    37681 agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa
    37741 atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat
    37801 gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct
    37861 tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc
    37921 tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg
    37981 caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg
    38041 tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta
    38101 ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct
    38161 gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt
    38221 agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca
    38281 agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac
    38341 ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat
    38401 ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag
    38461 gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca
    38521 gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa
    38581 gcttttggac accgtttacc
//
LOCUS       DAFEZB010000021        36853 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFEZB010000021
VERSION     DAFEZB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.021"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(324..893)
                     /locus_tag="LP859_001105"
     CDS             complement(324..893)
                     /locus_tag="LP859_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3029072.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="LP859_001106"
     CDS             complement(967..2256)
                     /locus_tag="LP859_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029073.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="LP859_001107"
     CDS             2610..3458
                     /locus_tag="LP859_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL3029074.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="LP859_001108"
     CDS             3472..4545
                     /locus_tag="LP859_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL3029075.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="LP859_001109"
     CDS             4535..6706
                     /locus_tag="LP859_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBL3029076.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="LP859_001110"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="LP859_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL3029077.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="LP859_001111"
     CDS             complement(9009..11195)
                     /locus_tag="LP859_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL3029078.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="LP859_001112"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="LP859_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL3029079.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="LP859_001113"
     CDS             complement(11417..11857)
                     /locus_tag="LP859_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL3029080.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="LP859_001114"
     CDS             complement(11931..12404)
                     /locus_tag="LP859_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HBL3029081.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="LP859_001115"
     CDS             complement(12714..14180)
                     /locus_tag="LP859_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3029082.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="LP859_001116"
     CDS             complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="LP859_001116"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL3029083.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(17099..17458)
                     /locus_tag="LP859_001117"
     CDS             complement(17099..17458)
                     /locus_tag="LP859_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL3029084.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17488..17829)
                     /locus_tag="LP859_001118"
     CDS             complement(17488..17829)
                     /locus_tag="LP859_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029085.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17826..18500)
                     /locus_tag="LP859_001119"
     CDS             complement(17826..18500)
                     /locus_tag="LP859_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL3029086.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="LP859_001120"
     CDS             complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="LP859_001120"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL3029087.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20633..21823)
                     /locus_tag="LP859_001121"
     CDS             complement(20633..21823)
                     /locus_tag="LP859_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3029088.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21844..22371)
                     /locus_tag="LP859_001122"
     CDS             complement(21844..22371)
                     /locus_tag="LP859_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL3029089.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22418..25207)
                     /locus_tag="LP859_001123"
     CDS             complement(22418..25207)
                     /locus_tag="LP859_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL3029090.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25354..25554)
                     /locus_tag="LP859_001124"
     CDS             complement(25354..25554)
                     /locus_tag="LP859_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3029091.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26006..26326)
                     /locus_tag="LP859_001125"
     CDS             complement(26006..26326)
                     /locus_tag="LP859_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029092.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(26627..27745)
                     /locus_tag="LP859_001126"
     CDS             complement(26627..27745)
                     /locus_tag="LP859_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029093.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            28216..29523
                     /locus_tag="LP859_001127"
     CDS             28216..29523
                     /locus_tag="LP859_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL3029094.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            29642..30841
                     /locus_tag="LP859_001128"
     CDS             29642..30841
                     /locus_tag="LP859_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL3029095.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(30868..32136)
                     /locus_tag="LP859_001129"
     CDS             complement(30868..32136)
                     /locus_tag="LP859_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL3029096.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(32299..32946)
                     /locus_tag="LP859_001130"
     CDS             complement(32299..32946)
                     /locus_tag="LP859_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBL3029097.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(33354..33857)
                     /locus_tag="LP859_001131"
     CDS             complement(33354..33857)
                     /locus_tag="LP859_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3029098.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(33980..34744)
                     /locus_tag="LP859_001132"
     CDS             complement(33980..34744)
                     /locus_tag="LP859_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL3029099.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            34851..35126
                     /locus_tag="LP859_001133"
     CDS             34851..35126
                     /locus_tag="LP859_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL3029100.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            35842..36792
                     /gene="yidC"
                     /locus_tag="LP859_001134"
     CDS             35842..36792
                     /gene="yidC"
                     /locus_tag="LP859_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL3029101.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT        10593 a         7759 c         6535 g        11966 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtgt gtattctttt
    14281 atttaataca catcgttttt tcatattttt tccatcaaaa aaataagtta ttttctttta
    14341 tttcattgac cttattgaat ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta
    14401 gttattttat aatcctatct attttcttta catttttcat taagaaaagg atataaaaaa
    14461 aagaggacgt tcattcgtcc tcttcattga tcaacgttta ttttttacat gcaagcttcg
    14521 catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg catctcccat
    14581 tgatgcaaaa ccaaaagcac ctaaggaaag taccaaaatt tttatcccaa tagcaaagac
    14641 aatgttctgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt
    14701 cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat cgcttcccaa
    14761 tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt tgattccatc
    14821 tccgacaaat gcggtctttt tattgttact ttgtaagatt tcctctaacc gctgcacttt
    14881 atcttctgga agcaattcgc tatatatctt gtccacgcct acttgcttcc cgatatgatc
    14941 agcaattttt ttcgagtcgc ccgtcaacat gactgtgttt ttcacaccag cctgttttaa
    15001 ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa gataaccact
    15061 gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga
    15121 gatttcaaat ctttccatca atttgtgatt acctactaat acttgtttgc catcaacttc
    15181 agctaagatc ccttcacctg caaattcctg gatattagct gttggtaaaa gcggacctgc
    15241 gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg cacttgcaac
    15301 atattgcata aatttctttg gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc
    15361 acctttcgtt aatgtgccag ttttatcgaa taccactgtc tcagtttgtg caagcaactc
    15421 caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa
    15481 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
    15541 tgctcgatag atccattcag aaaacgccgc tctagaaaca acaagtggag gtaaaacagc
    15601 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
    15661 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
    15721 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
    15781 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
    15841 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
    15901 aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg tcatcaatcc
    15961 ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
    16021 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
    16081 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
    16141 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
    16201 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
    16261 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
    16321 ctttttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
    16381 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
    16441 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
    16501 gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
    16561 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact
    16621 aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc
    16681 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
    16741 cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
    16801 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
    16861 tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac tacgctttca
    16921 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca
    16981 tataaataaa agacgtaaaa gtcacataga agaacttata caggaaaacg catagagtca
    17041 ggtgaaatac atgtatccca cctggctcta tgctttatct aacctttatt attctctttt
    17101 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
    17161 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
    17221 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
    17281 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
    17341 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
    17401 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
    17461 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
    17521 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
    17581 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc
    17641 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
    17701 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
    17761 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccttat
    17821 ttccatcatt tttcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
    17881 acaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
    17941 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatacca
    18001 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
    18061 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
    18121 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
    18181 gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
    18241 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
    18301 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
    18361 tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
    18421 ttacctagat cagttgcttc tgctcggtat cttcctgcgt ctgtaatgct atcaccgata
    18481 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
    18541 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
    18601 caccagccta gttccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg
    18661 ctattttttt cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga
    18721 tcgatcacac ccaagcttcc gtcaatcaga ccactgatga cgccatcgaa acatatattt
    18781 ataggctcat ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt
    18841 gggatagatt ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca
    18901 tatggattat tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt
    18961 tgttttcagg tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt
    19021 ccatttgaat taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg
    19081 tgaatcaaat agcaatttct tttgctttct tctcttgtct gctggaaact tccattatcc
    19141 cgtatttaaa aaaaagaact gcgagagttc gcagttcttt tttttaaata aacgttgctc
    19201 tccggccaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
    19261 gtgactcgga gctgaacggc aggcgcacaa cctcttatgg ataaattcgg ttcaacaaac
    19321 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    19381 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    19441 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    19501 ccgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    19561 cacaaattac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    19621 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    19681 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    19741 ttgcatcatc gtaagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    19801 tgtctcgtcc tagtacatct aatgcgtatt cgcaatgatc taaaacattt tgtactaaga
    19861 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    19921 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    19981 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    20041 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    20101 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    20161 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    20221 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    20281 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    20341 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    20401 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    20461 ggaaaaaggc tgttccgtct cttaattgta aaaatgtgat ttttccgctt gatcgcttat
    20521 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    20581 gaatttgttc cacgtatgac tctcctttta tattccttga atttcttcag gattaatttt
    20641 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    20701 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    20761 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    20821 tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc tccttgcggc ttttcaagag
    20881 aaacacctgg cagttctgcc acaagcggat ataacgtatt caggcgttct tcaaaggcgt
    20941 tccgcatttc ttctactgta tcttgctcac ctttcaatgc ttcaacagct gcatattgac
    21001 tgactgctgt cggattactg gttgactggg aagcaacagc aatcattcca ttgatgatct
    21061 cagaattccc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    21121 cattgatgat gatggtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    21181 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    21241 ttactgccca ttcaccgatt gcctgaagtt cattcttgct ataaatcatt ccagtcggat
    21301 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    21361 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    21421 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    21481 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    21541 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    21601 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    21661 cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    21721 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    21781 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    21841 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    21901 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    21961 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    22021 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    22081 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    22141 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
    22201 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    22261 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    22321 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    22381 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    22441 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
    22501 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
    22561 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    22621 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    22681 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    22741 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
    22801 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
    22861 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    22921 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    22981 cgatcagacc tgtaagtgct gtctctaaat aatcgatgta tgcttgattg ctgacttgat
    23041 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    23101 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
    23161 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    23221 cagtcgacgg caacatagcc gcttggaaat catggagatg aaaaatcatc tgctgcgctg
    23281 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    23341 tcagcaagta ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc
    23401 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    23461 gctgctgaat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    23521 gcagctgatc cagcatctct tttgtcatca tgacttcttc tggatactgt ccgttgggtt
    23581 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    23641 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    23701 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    23761 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    23821 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    23881 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    23941 aacgaacaaa gtcttgttcg taaaacgact gatttcttgc taaaaaccca gtacctctgt
    24001 gcgcgatatc tgcaaagaat gcatggttca agtttgttaa atgtaattca tccaaatcac
    24061 cggtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    24121 gttctggtgg ttccacaagt gttcctttaa atcgttgcag gtcgatatag tggcgataac
    24181 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatttctt
    24241 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    24301 gtgtagccaa ataactcaat ggcaacagat aaccgattgt tttacccatt cctgtagctg
    24361 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagatc gcattcatca
    24421 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa catcttttct tttgcttgtt
    24481 tttttcttgg atatgtccgc tcgccgtaat ggacggatgt gaatagttcc acttcttttt
    24541 tctgcaaagc tagcccatcg acgatttcta atgaggaatc aagtggctct ggtttttctt
    24601 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    24661 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    24721 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    24781 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    24841 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    24901 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    24961 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    25021 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    25081 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    25141 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    25201 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    25261 tgcagaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    25321 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    25381 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    25441 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    25501 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    25561 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    25621 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    25681 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    25741 gattacataa agtgaaaaaa cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    25801 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    25861 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    25921 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    25981 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    26041 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    26101 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    26161 cttctgtttc aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca
    26221 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    26281 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat
    26341 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    26401 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    26461 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    26521 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    26581 cctcttcctt aatactcagg tcaaaacact gatttcacaa cctcttttaa taacgaagaa
    26641 tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac actttcccgc
    26701 cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca tattctgttt
    26761 caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa tgtgacactt
    26821 ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc ttatctaaga
    26881 gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc ttcgcccctt
    26941 cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg tctgcacaat
    27001 aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaat gcaaataaat
    27061 aaatcggcca ttgttcagga ttttcgaaag aatcctttaa gtacgtatca acagcttggt
    27121 aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca tggaaagtta
    27181 ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca cctgtcagtt
    27241 cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat acttctttta
    27301 cttgtttgtt gctgacctca tacaatttca tggagtcgcg gttcaatccc aataaataat
    27361 aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga tcacttacat
    27421 agtattgatc gtctaccggg atactcaaac gatggatata ggtctggtca gcagttataa
    27481 tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac aaattttcaa
    27541 ttttttcttg gaacggagac cagtcatgtt cggaatattt tttctcaaaa cgtttctttg
    27601 cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga tgggcgacat
    27661 gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc aattgtgtca
    27721 ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca ttcctttgtt
    27781 tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc taaaaaaata
    27841 aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat aatttcctca
    27901 attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta agaaaaagaa
    27961 taaaagaggt ggatcttatg catttaatta tgattttcag tttattaagt attatcagtt
    28021 tatttgcttt cagcgcttcc cgcttattag atgcggaaac acggcctatt cctgtaaaag
    28081 tacgtaaaaa cgaccgctaa acttccataa cgcgcgttct tatcctaaag ttttttcaga
    28141 aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt
    28201 acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc
    28261 attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tacgttcaaa
    28321 gtaaatgcaa aagcagcttt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa
    28381 gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc
    28441 cttgatcaag taaaagaagg aaaactgtca tgggatgata aagtttcgat ttcagattat
    28501 gcagaaaatt tgagtatcac cccagattta tcgaatgttc ctttgcataa agaaaacacc
    28561 tacactgtca aagaattatt cgactctgca atcatccagt cagccaatgc ttcaatggtg
    28621 gccttggcag aaaaaatctc tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa
    28681 ttgaaagatt ggggaatcaa agatgcaaca atcgttaatg catcaggatt gaacaactct
    28741 tatcttggcg aaaaccgacc agaaggcact ggagaaaacg acgaaaatca aatgtctgct
    28801 caagatgtgg ccatcgttgc ccgccatttg atcttggatt tcccagaaat actggatgta
    28861 tcaagtacta cgacccaaat gtttggcgaa aatacgcaat caccagtgga aatggtcaac
    28921 tggaactgga tgctcccagg attcgttaat tacaaggaag gcgtggacgg attaaaaact
    28981 ggtacgactg agcttgcagg tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg
    29041 atcatcactg tcgtattgaa tgcggacggc catgcggaaa acccgagtgc ccgcttcaat
    29101 gaaacagacc gtttgatgga ctactgctat gacaattggt cagaaaaaga gctcggaaaa
    29161 gctaacgcaa gtattcctag tctaaaaacc attgatgtaa aagacggaaa agaatcaaca
    29221 gttcctgttg tgcttaaaag ccctgtaaaa gtatgggtac gaaacgatat ggatactggc
    29281 aaattaacga tcacgccaac acttgacaag aagcaagtca ctgacaatga gatccaagca
    29341 cctatggata aaggtacaaa aatcggtacc gctacgatca ccttggcaga cgataaatta
    29401 ggctatttag aagacaacaa tgcaccatca acagagatca ttacggataa aacagtggaa
    29461 aaagcgaata tattcgtgat tggctggcga cgcgtcgcag aatttttcga caacttgttt
    29521 taattgaaca ataagaaaaa catctttcca tcggaaagat gtttttttgc tttttctagt
    29581 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    29641 tatgaatcta accagagatc tgatcttttt agttctctat cttattgggg tattctctat
    29701 cgtttattgg cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac
    29761 atttacggta atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    29821 tttgattcca ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag
    29881 gctgctgaat ggtcttttat tcaatgtttt cctcatcagt ttcggcatct acttgtttgt
    29941 cttgctgtat gaaacgcaaa atattttcct tggcggactg atcgccttga tcacgattcc
    30001 attgctactt gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg
    30061 ggtcacagtt ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc
    30121 catttttctt acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg
    30181 gataaatgct ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta
    30241 caactttttg actgtctcat tcttgtatca gttcaaccgg cctcgataca atcaagactt
    30301 catcgttgtg ttaggcgctg ggttgatcaa tggagaaaca gtttctcctc ttctagccaa
    30361 gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc
    30421 catcttattg atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat
    30481 gaaacaatat gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc
    30541 gacaaccact ttggagaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg
    30601 gccataccgc gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc
    30661 gtggcaagcc aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc
    30721 taatgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    30781 catttgcgga ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta
    30841 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    30901 tattggcaaa aagagtatcc cttttttgac cgctacttgc tgggcagaat aaataccgct
    30961 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    31021 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    31081 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatgaagca aaggcgctag
    31141 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    31201 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    31261 tccattaaat gattcttgaa gtaacggaag actcaatccg agataacgtt gtgtttgcaa
    31321 aatcaatgct gctgccacaa ctacagctcc acctatcgta tttcgccaaa caggatgagg
    31381 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    31441 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    31501 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
    31561 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    31621 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    31681 actgattcca ctgataatca agatttcccg ttctgcttta tccaatcgga acaaacgtgc
    31741 tgtattatct gctaacgatc cccccatttg gactgctgtt ccttcacggc caacagaccc
    31801 cccaaataaa tgggtagtta tggttccaaa cagtgtaagt gtaataagcc gcaacgggat
    31861 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    31921 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    31981 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    32041 caagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    32101 taaaatgcgc attggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
    32161 acgcctacta aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg
    32221 ttagcattta aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt
    32281 ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg
    32341 agccaatttc gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt
    32401 tttcgtatac gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga
    32461 tgttttctga cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat
    32521 cccttttaat ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt
    32581 ccagccgatg tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt
    32641 ttttactttt cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac
    32701 gatttgcttc agcttctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa
    32761 aactgtcgtt aaaaatgtat cgatttctgg cagttcgtga tttaatatgt ctcgaatctt
    32821 ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc
    32881 atttaataac tgttctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg
    32941 ttccatgatt ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat
    33001 cttctgcaaa gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc
    33061 atcttatcat cacccccttt aatgcttatt cttctatgta catgattcat tcgataaata
    33121 aaagtaaacc gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa
    33181 agtcgtgtca aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcggtaa
    33241 taagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    33301 aagctgacca cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc
    33361 gaggattctt tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga
    33421 gccgcttctt tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac
    33481 ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc
    33541 gacaaatcgg tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt
    33601 gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca
    33661 gcacgcgctg ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa
    33721 gaaacactga tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat
    33781 tttttcactg cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca
    33841 cgccattttt ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa
    33901 attgatctaa gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta
    33961 aagcttaata gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc
    34021 cgacattcaa tgattccgca tttcctttca ttgggatata tacgttctga tcagccgaag
    34081 acaagatttc ctgacttact ccctgacctt cgtttcccat gattatagcg aaatcctctg
    34141 ttttttctac tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa
    34201 accctttgcc tttcattaca gggatcactt ctgccaaatc tttttgtaac acaggcaaat
    34261 gataattgct tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt
    34321 tacctaataa aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat
    34381 ttccaggatc ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt
    34441 cagatgttaa cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg
    34501 tcatgacctc atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc
    34561 cccattcttt catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg
    34621 cagcttcttc gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt
    34681 tctttttatg tagttttttt aattctttga ttcttggatt ttttactgat tggatctctt
    34741 tcacattcct cacctcaact gattatatca tggaataggt gcttgagctt gtttcatcct
    34801 aaaaaaatcg gtatcattga tttataaaac aggaaagaaa ggtgacgaac atgcgcaaag
    34861 ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt tcgctatatg accaagatgg
    34921 ttgctgatca gttaggcgtg accggaacgg taaaaaacga agacgacggc tcagtcacaa
    34981 tcgaagcaat cggaaatgac gacatcatcc aaaaatttat cgaagaagtc aagaagtctc
    35041 cgagcccaag tggacgagtg cagtatgtgg atgtccaaga agatccgatg attgaggaac
    35101 gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag cgctttgcct cgagtattaa
    35161 ggagaaacat tttccaaata gcttctcata ttttgaaatg taatgactta ataccgagag
    35221 gcagctgata aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag
    35281 gactggttgt gaaaaatagc tctccatatt tttcaacagg ctggacttaa tgccgtagat
    35341 gcaagctttt gaacaccgtc gataaggttg cgaaaaagct gatctgcatc aagcattaag
    35401 gactagttgt gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct
    35461 ttccacattt ttgcataaat ttgacttaat accaagaaac tgacttttga acatcgtcga
    35521 taaggttgtt tttgaggcac tttgcctcaa gtattaaaga caggtagtaa aaaagctgat
    35581 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    35641 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttctaagt atttctttct
    35701 tgattttttg tcttttttca aaatttttcg ctacgaaaaa gaacgaatat ttatatgaaa
    35761 tttctatgaa gttgattgaa ttccatgcgc ttttttagta tagttaagtt tgtgtaaaaa
    35821 ttatagaaga ggaagtattc tatgaataaa tggaaaaact ggttattggg atccggactt
    35881 gtcagtatcc tgctcttttt atctggctgt gtgcggatgg attcaaacgg aaaccctgac
    35941 acttcaggaa tcgtttatcg agtactcgtc catcctatgg gccaagcgat tacatatcta
    36001 gttgagaatt tcaattggtc ttacggctgg gcagttattg caatgactgt gatcgttcgt
    36061 atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa caatgatcca atctgaaaaa
    36121 atgcaagcgt taaaaccaca agttgaagca gcacaacaaa agctaaaagc tgcaacaaca
    36181 cgtgaagaac aaatggcagc acaagcagaa atgcagcaag tatatcgcga aaatggattg
    36241 agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat cttctctgcc
    36301 ttgtatttta cggctcgcta cacagaaggt atccgtgaat catcttttta cggcatcgat
    36361 ctaggctcac caagtatggt gcttgtggcc atcgcagggg tcgcttatct gcttcaagga
    36421 tatatttcaa caatcggtat tcctgaagaa cagaaaaaga cgatgcgtac gatgctgatc
    36481 gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc cagccggtgt tacactttac
    36541 tgggtagtcg gtggtgtgtt cagctgtctg caaacattca tcacaaacgt tataatgaaa
    36601 ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa acccgccaaa acaagtcgtt
    36661 actccaagaa aagacgtgac agaatcagca aagccagctg acaacaaaca agcaacaaaa
    36721 caaatcaaac aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg aaagcagcag
    36781 aagcggaaat aaaaatatat gacaaaaaac tgtagacaaa taaaaaaatt tctttgttct
    36841 acagtttttt tgt
//
LOCUS       DAFEZB010000022        36570 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFEZB010000022
VERSION     DAFEZB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.022"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LP859_001135"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LP859_001135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL3029102.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LP859_001136"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LP859_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL3029103.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LP859_001137"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LP859_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL3029104.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LP859_001138"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LP859_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL3029105.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LP859_001139"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LP859_001139"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL3029106.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LP859_001140"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LP859_001140"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL3029107.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="LP859_001141"
     CDS             complement(8054..8524)
                     /locus_tag="LP859_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL3029108.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="LP859_001142"
     CDS             complement(8570..8995)
                     /locus_tag="LP859_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL3029109.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="LP859_001143"
     CDS             complement(9062..9331)
                     /locus_tag="LP859_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL3029110.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="LP859_001144"
     CDS             complement(9342..10937)
                     /locus_tag="LP859_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3029111.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LP859_001145"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LP859_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL3029112.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="LP859_001146"
     CDS             complement(14490..15050)
                     /locus_tag="LP859_001146"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL3029113.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="LP859_001147"
     CDS             15403..16377
                     /locus_tag="LP859_001147"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL3029114.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="LP859_001148"
     CDS             16547..17947
                     /locus_tag="LP859_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL3029115.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="LP859_001149"
     CDS             complement(18025..18747)
                     /locus_tag="LP859_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3029116.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="LP859_001150"
     CDS             complement(18826..19332)
                     /locus_tag="LP859_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL3029117.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="LP859_001151"
     CDS             19455..21254
                     /locus_tag="LP859_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL3029118.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="LP859_001152"
     CDS             complement(21322..22896)
                     /locus_tag="LP859_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL3029119.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LP859_001153"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LP859_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL3029120.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LP859_001154"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LP859_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL3029121.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="LP859_001155"
     CDS             complement(24673..25311)
                     /locus_tag="LP859_001155"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL3029122.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="LP859_001156"
     CDS             complement(25312..25962)
                     /locus_tag="LP859_001156"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL3029123.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LP859_001157"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LP859_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL3029124.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LP859_001158"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LP859_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL3029125.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="LP859_001159"
     CDS             complement(29105..29845)
                     /locus_tag="LP859_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL3029126.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LP859_001160"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LP859_001160"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL3029127.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="LP859_001161"
     CDS             complement(31216..32154)
                     /locus_tag="LP859_001161"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL3029128.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="LP859_001162"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="LP859_001162"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL3029129.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LP859_001163"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LP859_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL3029130.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="LP859_001164"
     CDS             complement(35314..35619)
                     /locus_tag="LP859_001164"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL3029131.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LP859_001165"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LP859_001165"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL3029132.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DAFEZB010000023        36376 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFEZB010000023
VERSION     DAFEZB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.023"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1617
                     /gene="uvrC"
                     /locus_tag="LP859_001166"
     CDS             <1..1617
                     /gene="uvrC"
                     /locus_tag="LP859_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL3029133.1"
                     /translation="RLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKT
                     YPFLKITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQT
                     KEPCLYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENM
                     EFEKAAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIE
                     RDVSMFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPK
                     RGEKKKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNS
                     NIMGTNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLP
                     LPDLILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLER
                     NSPEFFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLK
                     NITAATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            1762..2661
                     /locus_tag="LP859_001167"
     CDS             1762..2661
                     /locus_tag="LP859_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029134.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            2654..3916
                     /locus_tag="LP859_001168"
     CDS             2654..3916
                     /locus_tag="LP859_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029135.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            4077..5975
                     /locus_tag="LP859_001169"
     CDS             4077..5975
                     /locus_tag="LP859_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL3029136.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(6305..7024)
                     /locus_tag="LP859_001170"
     CDS             complement(6305..7024)
                     /locus_tag="LP859_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL3029137.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            7361..7693
                     /locus_tag="LP859_001171"
     CDS             7361..7693
                     /locus_tag="LP859_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029138.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            7680..9683
                     /locus_tag="LP859_001172"
     CDS             7680..9683
                     /locus_tag="LP859_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL3029139.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            9702..10172
                     /locus_tag="LP859_001173"
     CDS             9702..10172
                     /locus_tag="LP859_001173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL3029140.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            10193..10777
                     /locus_tag="LP859_001174"
     CDS             10193..10777
                     /locus_tag="LP859_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL3029141.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            10792..11778
                     /locus_tag="LP859_001175"
     CDS             10792..11778
                     /locus_tag="LP859_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL3029142.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            11768..12079
                     /locus_tag="LP859_001176"
     CDS             11768..12079
                     /locus_tag="LP859_001176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL3029143.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            12101..13882
                     /locus_tag="LP859_001177"
     CDS             12101..13882
                     /locus_tag="LP859_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL3029144.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            13875..15251
                     /locus_tag="LP859_001178"
     CDS             13875..15251
                     /locus_tag="LP859_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL3029145.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            15258..15893
                     /locus_tag="LP859_001179"
     CDS             15258..15893
                     /locus_tag="LP859_001179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL3029146.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            15923..17278
                     /locus_tag="LP859_001180"
     CDS             15923..17278
                     /locus_tag="LP859_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL3029147.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(17470..18864)
                     /locus_tag="LP859_001181"
     CDS             complement(17470..18864)
                     /locus_tag="LP859_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL3029148.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            18956..19927
                     /locus_tag="LP859_001182"
     CDS             18956..19927
                     /locus_tag="LP859_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL3029149.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            19924..20706
                     /locus_tag="LP859_001183"
     CDS             19924..20706
                     /locus_tag="LP859_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL3029150.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(20875..21246)
                     /locus_tag="LP859_001184"
     CDS             complement(20875..21246)
                     /locus_tag="LP859_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3029151.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            21380..21823
                     /locus_tag="LP859_001185"
     CDS             21380..21823
                     /locus_tag="LP859_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL3029152.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(21866..22675)
                     /gene="yidA"
                     /locus_tag="LP859_001186"
     CDS             complement(21866..22675)
                     /gene="yidA"
                     /locus_tag="LP859_001186"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL3029153.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(22694..24061)
                     /locus_tag="LP859_001187"
     CDS             complement(22694..24061)
                     /locus_tag="LP859_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3029154.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(24045..24875)
                     /locus_tag="LP859_001188"
     CDS             complement(24045..24875)
                     /locus_tag="LP859_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL3029155.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            25038..25475
                     /locus_tag="LP859_001189"
     CDS             25038..25475
                     /locus_tag="LP859_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL3029156.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            25551..26171
                     /locus_tag="LP859_001190"
     CDS             25551..26171
                     /locus_tag="LP859_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL3029157.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            26360..26866
                     /locus_tag="LP859_001191"
     CDS             26360..26866
                     /locus_tag="LP859_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL3029158.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            26883..27782
                     /locus_tag="LP859_001192"
     CDS             26883..27782
                     /locus_tag="LP859_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL3029159.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            27869..28753
                     /locus_tag="LP859_001193"
     CDS             27869..28753
                     /locus_tag="LP859_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL3029160.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(28945..29016)
                     /locus_tag="LP859_001194"
     tRNA            complement(28945..29016)
                     /locus_tag="LP859_001194"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28981..28983),aa:Arg,seq:ccg)
     gene            29076..30410
                     /gene="rpoN"
                     /locus_tag="LP859_001195"
     CDS             29076..30410
                     /gene="rpoN"
                     /locus_tag="LP859_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL3029161.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            30559..31650
                     /locus_tag="LP859_001196"
     CDS             30559..31650
                     /locus_tag="LP859_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL3029162.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            31691..32692
                     /gene="gap"
                     /locus_tag="LP859_001197"
     CDS             31691..32692
                     /gene="gap"
                     /locus_tag="LP859_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL3029163.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            32802..33992
                     /locus_tag="LP859_001198"
     CDS             32802..33992
                     /locus_tag="LP859_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL3029164.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            34096..34851
                     /locus_tag="LP859_001199"
     CDS             34096..34851
                     /locus_tag="LP859_001199"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL3029165.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYETGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            34967..36265
                     /gene="eno"
                     /locus_tag="LP859_001200"
     CDS             34967..36265
                     /gene="eno"
                     /locus_tag="LP859_001200"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL3029166.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        12045 a         6368 c         7745 g        10218 t
ORIGIN      
        1 cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca
       61 ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag
      121 gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt
      181 actcgtaagg tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg
      241 gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt
      301 cagacgaagg agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc
      361 gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga
      421 ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg
      481 gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact
      541 cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat
      601 aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat
      661 gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag
      721 ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac
      781 aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag
      841 aaaaaattag ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat
      901 ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg
      961 aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat
     1021 cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa
     1081 tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat
     1141 cgcagatatt cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat
     1201 ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat
     1261 attcctattg ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt
     1321 acagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga
     1381 atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac
     1441 agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg
     1501 cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa
     1561 atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga
     1621 aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt
     1681 ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt
     1741 tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac
     1801 tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt
     1861 ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa
     1921 gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc
     1981 ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt
     2041 tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg
     2101 atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga
     2161 aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg
     2221 gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg
     2281 gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa
     2341 aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta
     2401 catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa
     2461 caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta
     2521 gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc
     2581 ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa
     2641 gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt
     2701 taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta
     2761 tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga
     2821 taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc
     2881 agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt
     2941 agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg
     3001 acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc
     3061 gagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac
     3121 tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac
     3181 agtccaatac gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta
     3241 tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat
     3301 cctgtccagt acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt
     3361 tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa
     3421 agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc
     3481 attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc
     3541 agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt
     3601 gacttatcta tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa
     3661 caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat
     3721 tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt
     3781 agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt
     3841 ttatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga
     3901 acgaaaaaaa gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc
     3961 agcttctttt tgtatagacc tattttattt attttactat attggatata tattcattga
     4021 aaatagtgta taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg
     4081 accgttattt agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa
     4141 tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata
     4201 tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag
     4261 aaaagatcaa agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta
     4321 tttttacggc ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat
     4381 cgcagctgat ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt
     4441 caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata
     4501 tcgatgaccg tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt
     4561 tgatgaagga agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca
     4621 tcgatcattt gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga
     4681 tggatcgaat catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt
     4741 ggattggtgc gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac
     4801 gctataatta tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt
     4861 cttttgctca tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc
     4921 tatccgaaag agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat
     4981 tcaaatcaga acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat
     5041 tgctggaaaa aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt
     5101 tgaaaggaac ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg
     5161 aacaaaaagt gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac
     5221 atatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt
     5281 ttcatggctg tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat
     5341 cttatcgcgg aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag
     5401 atctgactgc aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat
     5461 tttcgccttt attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc
     5521 ctcatacgca cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca
     5581 gtgaaaaaat tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc
     5641 acacgccagt aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg
     5701 tcattgacgg aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac
     5761 tattatgcaa ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac
     5821 aacaatttgc aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac
     5881 cacgcaaact caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc
     5941 tcaaaaggct gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc
     6001 atagactgga gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt
     6061 cttctgccaa ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt
     6121 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg
     6181 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt
     6241 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag
     6301 tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt
     6361 gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa
     6421 ccgcttcaac gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt
     6481 tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca
     6541 aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt
     6601 catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa
     6661 cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg
     6721 cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg
     6781 gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt
     6841 tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag
     6901 gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat
     6961 ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt
     7021 tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta
     7081 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt
     7141 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
     7201 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
     7261 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
     7321 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
     7381 tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca
     7441 gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta
     7501 tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga
     7561 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca
     7621 atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta
     7681 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
     7741 ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa
     7801 caaacaacca gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa
     7861 aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac
     7921 accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag
     7981 atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt
     8041 taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat
     8101 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
     8161 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta
     8221 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
     8281 atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca
     8341 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc
     8401 aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg
     8461 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa
     8521 aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg
     8581 cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat
     8641 acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
     8701 aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
     8761 atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga
     8821 tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac
     8881 tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac
     8941 catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac
     9001 tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
     9061 tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa
     9121 aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag
     9181 ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat
     9241 ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga
     9301 ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac
     9361 tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag
     9421 gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat
     9481 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc
     9541 taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata
     9601 caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc
     9661 acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat tacttaatta
     9721 gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag
     9781 ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa
     9841 gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt
     9901 tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg
     9961 tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag
    10021 gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac
    10081 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg
    10141 ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    10201 cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga
    10261 acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac
    10321 agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct
    10381 aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    10441 aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat
    10501 ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc
    10561 tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact
    10621 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    10681 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    10741 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    10801 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    10861 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    10921 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    10981 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    11041 tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat
    11101 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    11161 gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa
    11221 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    11281 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    11341 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    11401 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    11461 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    11521 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt
    11581 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    11641 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    11701 ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga
    11761 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    11821 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    11881 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    11941 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    12001 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    12061 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    12121 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    12181 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    12241 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    12301 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    12361 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    12421 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    12481 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    12541 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    12601 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    12661 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    12721 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    12781 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    12841 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    12901 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    12961 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    13021 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    13081 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    13141 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    13201 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    13261 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    13321 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    13381 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    13441 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    13501 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    13561 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    13621 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    13681 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    13741 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    13801 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    13861 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    13921 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    13981 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    14041 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    14101 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    14161 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    14221 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    14281 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    14341 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    14401 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    14461 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    14521 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    14581 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    14641 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    14701 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    14761 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    14821 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    14881 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    14941 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    15001 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    15061 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    15121 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    15181 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    15241 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    15301 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    15361 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    15421 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    15481 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    15541 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    15601 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    15661 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    15721 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    15781 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    15841 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    15901 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    15961 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    16021 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    16081 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    16141 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    16201 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    16261 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    16321 cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt
    16381 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    16441 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    16501 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    16561 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    16621 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    16681 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    16741 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    16801 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    16861 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    16921 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    16981 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    17041 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    17101 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    17161 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    17221 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    17281 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    17341 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    17401 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt
    17461 atcaataggt tataattcat atttttttat tttttctttt acgatactta gtccagaagc
    17521 taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg
    17581 tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat
    17641 tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt
    17701 tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa
    17761 tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac
    17821 atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac
    17881 atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc
    17941 agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg
    18001 atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc
    18061 aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt
    18121 acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat
    18181 atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg
    18241 agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga
    18301 gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga
    18361 ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa
    18421 atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt
    18481 tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa
    18541 atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg
    18601 agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt
    18661 ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga
    18721 gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat
    18781 caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta
    18841 attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac catacaattt
    18901 taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa
    18961 aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat
    19021 gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga
    19081 aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga
    19141 gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt
    19201 accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt
    19261 gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga
    19321 ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt
    19381 gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat
    19441 tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac
    19501 gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt
    19561 gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa
    19621 tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc
    19681 tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc
    19741 catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc
    19801 tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct
    19861 agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt
    19921 aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat
    19981 ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc
    20041 attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt
    20101 agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca
    20161 tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc
    20221 ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg
    20281 ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa
    20341 gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg
    20401 cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca
    20461 gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa
    20521 gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat
    20581 aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta
    20641 aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt
    20701 atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta
    20761 caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt
    20821 ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc
    20881 tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt
    20941 gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt gctcgtgtaa
    21001 tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc
    21061 accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata
    21121 aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc
    21181 cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat
    21241 tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa
    21301 tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact
    21361 gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga
    21421 ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat
    21481 cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa
    21541 agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc
    21601 ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa
    21661 tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac
    21721 caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca
    21781 ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc
    21841 agctttctct cagttttttt atttattaca gtacatattt tttgattgct tcagctacac
    21901 cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga gaaacggcat
    21961 ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc tcgttatctc
    22021 cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc gcaactgccg
    22081 cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt ttgaccgttg
    22141 tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt
    22201 cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca ggtgttcgat
    22261 attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg atatctcgat
    22321 taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca cggcgtgcca
    22381 ttacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg attttacctg
    22441 atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta tcttgaaaaa
    22501 ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg cataaaacga
    22561 tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt gttacttctc
    22621 ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata gacataattt
    22681 cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg ctggcaaatt
    22741 cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca accatttctt
    22801 ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca acaagctgac
    22861 taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat
    22921 gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa ttcacagcag
    22981 tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct tggactaatt
    23041 ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc aaaaaacgtt
    23101 tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag taagtgccca
    23161 agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca agaaatggca
    23221 gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc
    23281 gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg aagtacactt
    23341 gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg ccactcatgg
    23401 aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta agatcaaatg
    23461 ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt cgatctgcat
    23521 cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt tggatcacac
    23581 tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac acttctgttt
    23641 ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag atatgttcaa
    23701 atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat aaagtcacca
    23761 gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa
    23821 agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta aaacggctat
    23881 gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt
    23941 ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg atatagttat
    24001 ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa ggggttgtca
    24061 taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt gttccattgc
    24121 tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact tgcagaatac
    24181 agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag gggttggtca
    24241 agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc atttgtacca
    24301 aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccacg tttttcctga
    24361 ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg acgctgagcg
    24421 ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca ataagaatgg
    24481 gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa taggagcatt
    24541 tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa aatcaaagaa
    24601 atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct gatcactgga
    24661 gcgtaacctg catcttccag tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc
    24721 ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt
    24781 tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc gtttttttga
    24841 taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt gtataaattc
    24901 atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa aggttttctg
    24961 taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta gctatgagaa
    25021 gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc aaattgcgta
    25081 caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa gggcaaatgg
    25141 tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag acgggcgatg
    25201 agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc cgtgcaggag
    25261 aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc tatcgaacac
    25321 catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt ttgaaagacc
    25381 gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg
    25441 gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt ttgatttttt
    25501 gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga ttggaaatta
    25561 gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca cacgcgatct
    25621 tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc caaaactatc
    25681 ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact gacttgaata
    25741 ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca tggtatgcta
    25801 tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa gatactccac
    25861 gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt
    25921 acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat gatctgtttg
    25981 atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg caagaaatcg
    26041 gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa gaagatttaa
    26101 gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt tctgactctg
    26161 aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca gcctatcaga
    26221 ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa aatcccaagt
    26281 aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc atattaagtg
    26341 aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc agatcgaaaa
    26401 ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt tccgtatcac
    26461 gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata gttttcataa
    26521 agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat ggttttatcg
    26581 gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc ctgtggactt
    26641 atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg cagagctcca
    26701 tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct acttttttga
    26761 aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca cagttaaaac
    26821 gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga gagaagagtg
    26881 ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa gaacaagcaa
    26941 ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag caatcattta
    27001 taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg gctatcgtaa
    27061 tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca gaagatttac
    27121 ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat cgatctgaaa
    27181 aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat aaaacaattc
    27241 atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag
    27301 aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag aaaatcgtag
    27361 acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca gatagccatg
    27421 ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca cttccacttt
    27481 tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg cagatggccg
    27541 aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt caagaaggga
    27601 cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac aaagagcatc
    27661 aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt acaccagaat
    27721 ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat ttggatcatt
    27781 agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag taaataataa
    27841 gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta tcagtattat
    27901 tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga tcaccgaagt
    27961 aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat cagaacaatc
    28021 aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc aagaatcagc
    28081 ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa cgagccgaga
    28141 tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga ccacaacacc
    28201 gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga
    28261 aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc acgagattgg
    28321 caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga tgacggagga
    28381 agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca cttttgttcg
    28441 tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac cgatcatcga
    28501 atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt tgcagaagat
    28561 ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc ctgaaacgat
    28621 tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca tcacgacagt
    28681 tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa acgatcatcg
    28741 gccagttcag taagtgagac aagtatcttt aataaataac acattttaat aagtagttga
    28801 acacataact accttacttg caaaggctta acaatatcta tacaggtaca acaaaaaact
    28861 gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca actattcgta
    28921 aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg tctcaagaac
    28981 cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa ttgtaactaa
    29041 acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa aatttttcac
    29101 aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag tccatccaag
    29161 ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca atggaaaatc
    29221 cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct tcttcatctt
    29281 cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa gggtctttat
    29341 ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg aggaaaatcg
    29401 ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt ttagaagaag
    29461 ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact ctgattcagc
    29521 aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag
    29581 aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt tttgaggaac
    29641 tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta catgctgttc
    29701 agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt ttcgaccgta
    29761 gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat aaaaatgtac
    29821 aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt tacttcaaac
    29881 aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg aaacaggaat
    29941 tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg gttgctcaag
    30001 tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc aagcctctta
    30061 cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt cgtgcagtca
    30121 acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt ttcactacac
    30181 gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca gcgaagaaaa
    30241 agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac cagaaattgg
    30301 ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca aaatatcgtg
    30361 atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa
    30421 gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag agcaaaactg
    30481 ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc gaatagttaa
    30541 tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa gtgtccaact
    30601 aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc agatattatc
    30661 gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca gccgattgga
    30721 cgtcgaagtt tatcggaaag tatggggatc acagaacgag tattgcgaac agaaacagat
    30781 cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct gacaactaag
    30841 ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg tacgcatcaa
    30901 accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt ttcaggaaac
    30961 agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga agccctagac
    31021 cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac tatggctgtg
    31081 gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt tgtccctgca
    31141 agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc aagaatggct
    31201 gctaaagcaa acggtaacca ccgtgcactt tatgttccgg aacaattaag tctggcaact
    31261 tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat tagtgaagcg
    31321 aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg taagatgact
    31381 gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt tggctacttt
    31441 ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga attgcaagat
    31501 ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc taaagcaatt
    31561 cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga agctgctgca
    31621 aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt tcataaggag
    31681 gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg gacgtctagc
    31741 attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg acttgacaga
    31801 tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt tcaacggaac
    31861 agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag tattagctaa
    31921 ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc tagaatgtac
    31981 tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg ctaaacgcgt
    32041 tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca caaaccacga
    32101 aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta actgtttagc
    32161 tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa tgacaactat
    32221 ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag gtgacttccg
    32281 ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg ctaaagctat
    32341 cggtttagta attcctgaat tgaacggtaa attagacgga gctgctcaac gtgttcctgt
    32401 accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa ctgttgacga
    32461 aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca cagacgaaat
    32521 cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta cacaaactaa
    32581 agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg acaacgaaat
    32641 gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat aagatagaca
    32701 aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa tcgcgcttct
    32761 tccgcttttt ttgataataa gtttattcag gaggaaataa aatggctaag aaaacagtga
    32821 aagatatcga tttaaaagac aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga
    32881 aagacggcgt gatcactgac gacactcgta tcaaagcagc attgccaaca atcaactacg
    32941 ttctagaaca aggcggaaaa gcaatccttt tctctcacct tggacgtgta aaaacagaag
    33001 aagataaaga aggaaaatct ttagctccag ttgcaaaacg tctaggcgaa ttattaggta
    33061 aagaagtaac attcgtacct gaaactcgcg gagaacaatt agaagaagca atccgcaaca
    33121 tgaatgacgg cgacgttgtc gtattcgaaa atacacgatt tgaagatatt gacggtaaaa
    33181 aagaaagcaa aaatgataca gaactaggta aatactgggc ttcattaggc gatgtatttg
    33241 ttaacgatgc tttcggtaca gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa
    33301 ctggtatccc aacagttgct ggattcttga tggaaaaaga aatcaagttc atcggtgaag
    33361 cagtagaaga accaaaacga ccaatgattg caatccttgg tggcgcaaaa gtttctgata
    33421 aaatcggcgt aatcgaaaac ttgattccta aagcagataa aatcttgatc ggtggcggaa
    33481 tgacttacac attctacgaa gcaaaaggaa tcaagatcgg taactctcta gtagaagctg
    33541 ataaagtgga actagcaaaa gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa
    33601 tcgacaatgt ctgtgcacca gaattttcaa atgatgttga aacacaagta atcgaaggag
    33661 acattcctga tggtttgatg gctttagaca tcggcccagc atcagtaaaa ttatttgctg
    33721 atacattaca aggtgcaaaa acagttgttt ggaacggacc tatgggtgta tttgaaatga
    33781 gtaactttgc taaaggtaca atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa
    33841 caacaatcat cggtggcgga gactcagctg cagcagcaga acaattagga tttgctgaca
    33901 aattcacaca catctcaaca ggtggtggcg caagcttgga attgcttgaa ggtaaagaat
    33961 taccaggact agcagcaatc aacgataaat aaacgaccaa gtttgctcaa ttctgacgag
    34021 taatcgatca aaaggtgaag ggagttttca tgactcccta gccatttttc aataaattaa
    34081 ggagtgtgtt cctcaatgcg taaacctatt atcgcaggta actggaaaat gaacaaaact
    34141 gcatcagaag caaaagaatt tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa
    34201 gttgattcag tcatcggatc tcctgcatta ttcttacaag aattaatgac atcagctgaa
    34261 ggtacagaac taaaaatctc agcacaaaac tgctactggg aaaactcagg tgcgttcact
    34321 ggtgaaactt caccagcagc tcttgctgat ctaggtgttc attatgtgat catcggacat
    34381 tcagaacgtc gtgaatattt ccatgaaaca gacgaagaca tcaataaaaa agcaaaagct
    34441 atctttgcaa atggtatgac accaatccta tgctgcggcg aatctttaga aacctatgaa
    34501 acaggcaaaa ctgctgaatg gatcgaagga caaattacag ctggtttagc tgatctttca
    34561 gctgaacaag tgtcaaacat ggttattgct tatgaaccaa tctgggcaat cggtactggt
    34621 aaatcagctg acgcacaaat cgctgacgaa atttgtggag ttgtacgtca aacagttgaa
    34681 aaactttatg gaaaagaagt atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa
    34741 ccagaaaata ttgctgaata catggcaaaa gaaaatgttg acggtgcttt agtcggtggc
    34801 gcaagcttgc aagcagattc atttttgtca ttgttagacg ctgtaaaata attgttgaaa
    34861 atttgcgaaa aatgcaagaa atgtttgcac taatgagcaa aattaactaa aatcgtatat
    34921 gagggaaatc ccttaggaaa aaacaaactc aaaggagaga caaaacatgt caattattac
    34981 tgatgtttac gctcgcgaga tcttagactc acgtggtaac ccaacaatcg aagtagaagt
    35041 atacacagaa agcggagctt ttggccgcgg aatggttcct tcaggtgctt caactggtga
    35101 atacgaagca gttgaattac gtgacggaga caaagctcgt tatggcggta aaggtgtaac
    35161 taaagctgtc gacaacgtaa acaacatcat tgctgaagca atcattggct acgatgttcg
    35221 tgaccaaatg gctatcgaca aagctatgat cgctttagac ggaactccta acaaaggtaa
    35281 attaggtgcg aacgctattt taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta
    35341 cctagaagta cctttatacc actacctagg cggattcaac acaaaagtat tgccaacacc
    35401 aatgatgaac atcatcaacg gcggatcaca tgctgataac tcaatcgact tccaagaatt
    35461 catgatcatg cctgttggcg ctccaacatt caaagaagct ctacgtatgg gtgctgaagt
    35521 attccacgca ttagcttcaa tcttgaaagc tcgcggatta gcaacttctg ttggtgacga
    35581 aggtggattt gctccaaacc ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc
    35641 aatcgaaaaa gctggctatg tacctggtaa agacgttgtt cttgctatgg atgctgcttc
    35701 ttcagaattc tacgacaaag aaaaaggtgt ttacgtacta gctgactcag gcgaaggcga
    35761 aaaaacaact gacgaaatga tcaaattcta cgaagaatta gtttctaaat atccaatcat
    35821 ctcaatcgaa gatggattag acgaaaacga ctgggacgga ttcaagaaat taactgacgt
    35881 attaggcgac aaagttcaat tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt
    35941 atctgaaggt atcgaaaaag gaattgctaa ctcaatcctt atcaaagtga accaaatcgg
    36001 tacattaaca gaaactttcg aagctatcga aatggctaaa gaagctggct acacagcagt
    36061 tgtatctcac cgttctggtg aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac
    36121 aaatgctggc caaatcaaaa ctggttctct atcacgtact gaccgtattg ctaaatacaa
    36181 ccaattacta cgtatcgaag accaacttgg tgaagttgca gaatacaaag gtttgaaatc
    36241 tttctacaac ttaaaaaaca aataattctt ttctaatgaa gatacatgat cgactattga
    36301 aagatagtcg agtatgatca cttagttaag taatcgaact ctctacttgc tgaaatatca
    36361 gcaggtagag agtttt
//
LOCUS       DAFEZB010000024        35882 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFEZB010000024
VERSION     DAFEZB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.024"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1152
                     /locus_tag="LP859_001201"
     CDS             <1..1152
                     /locus_tag="LP859_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBL3029167.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LP859_001202"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LP859_001202"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL3029168.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LP859_001203"
     CDS             2728..3003
                     /locus_tag="LP859_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL3029169.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LP859_001204"
     CDS             3009..3776
                     /locus_tag="LP859_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL3029170.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LP859_001205"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LP859_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL3029171.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LP859_001206"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LP859_001206"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL3029172.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="LP859_001207"
     CDS             7920..8222
                     /locus_tag="LP859_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL3029173.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="LP859_001208"
     CDS             8225..9388
                     /locus_tag="LP859_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL3029174.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="LP859_001209"
     CDS             9414..12842
                     /locus_tag="LP859_001209"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL3029175.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="LP859_001210"
     CDS             12863..13987
                     /locus_tag="LP859_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029176.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="LP859_001211"
     CDS             13977..14414
                     /locus_tag="LP859_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL3029177.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="LP859_001212"
     CDS             14398..14736
                     /locus_tag="LP859_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL3029178.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="LP859_001213"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="LP859_001213"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL3029179.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="LP859_001214"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="LP859_001214"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL3029180.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="LP859_001215"
     CDS             15893..16936
                     /locus_tag="LP859_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL3029181.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="LP859_001216"
     CDS             17095..17823
                     /locus_tag="LP859_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL3029182.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="LP859_001217"
     CDS             17939..18184
                     /locus_tag="LP859_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL3029183.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="LP859_001218"
     CDS             18181..18399
                     /locus_tag="LP859_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL3029184.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="LP859_001219"
     CDS             18535..19074
                     /locus_tag="LP859_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL3029185.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="LP859_001220"
     CDS             19067..21340
                     /locus_tag="LP859_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL3029186.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="LP859_001221"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="LP859_001221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL3029187.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="LP859_001222"
     CDS             complement(22513..22767)
                     /locus_tag="LP859_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL3029188.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="LP859_001223"
     CDS             complement(23167..23436)
                     /locus_tag="LP859_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029189.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="LP859_001224"
     CDS             complement(23906..24361)
                     /locus_tag="LP859_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL3029190.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="LP859_001225"
     CDS             complement(24499..26427)
                     /locus_tag="LP859_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL3029191.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="LP859_001226"
     CDS             complement(26624..27457)
                     /locus_tag="LP859_001226"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL3029192.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="LP859_001227"
     CDS             complement(27649..28353)
                     /locus_tag="LP859_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3029193.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="LP859_001228"
     CDS             complement(28376..29002)
                     /locus_tag="LP859_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3029194.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="LP859_001229"
     CDS             29328..31703
                     /locus_tag="LP859_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL3029195.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="LP859_001230"
     CDS             complement(31727..32218)
                     /locus_tag="LP859_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL3029196.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="LP859_001231"
     CDS             32405..33325
                     /locus_tag="LP859_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3029197.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="LP859_001232"
     CDS             33479..33823
                     /locus_tag="LP859_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL3029198.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="LP859_001233"
     CDS             complement(34013..34513)
                     /locus_tag="LP859_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029199.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="LP859_001234"
     tRNA            35041..35124
                     /locus_tag="LP859_001234"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="LP859_001235"
     CDS             complement(35448..>35882)
                     /locus_tag="LP859_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL3029200.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6261 c         7405 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAFEZB010000025        34636 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFEZB010000025
VERSION     DAFEZB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.025"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            591..1280
                     /locus_tag="LP859_001236"
     CDS             591..1280
                     /locus_tag="LP859_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029201.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LP859_001237"
     CDS             1267..2475
                     /locus_tag="LP859_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3029202.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LP859_001238"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LP859_001238"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL3029203.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LP859_001239"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LP859_001239"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL3029204.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LP859_001240"
     CDS             complement(6578..7273)
                     /locus_tag="LP859_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL3029205.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LP859_001241"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LP859_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL3029206.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LP859_001242"
     CDS             complement(8472..8663)
                     /locus_tag="LP859_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL3029207.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LP859_001243"
     CDS             complement(8684..9574)
                     /locus_tag="LP859_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL3029208.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LP859_001244"
     CDS             complement(9561..10328)
                     /locus_tag="LP859_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3029209.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LP859_001245"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LP859_001245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL3029210.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LP859_001246"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LP859_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL3029211.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LP859_001247"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LP859_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL3029212.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LP859_001248"
     CDS             14899..15282
                     /locus_tag="LP859_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029213.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LP859_001249"
     CDS             complement(15450..16208)
                     /locus_tag="LP859_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL3029214.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LP859_001250"
     CDS             complement(16226..17044)
                     /locus_tag="LP859_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL3029215.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LP859_001251"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LP859_001251"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL3029216.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LP859_001252"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LP859_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL3029217.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LP859_001253"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LP859_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL3029218.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LP859_001254"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LP859_001254"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL3029219.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="LP859_001255"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="LP859_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL3029220.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="LP859_001256"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="LP859_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL3029221.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="LP859_001257"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="LP859_001257"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL3029222.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="LP859_001258"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="LP859_001258"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL3029223.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="LP859_001259"
     CDS             27086..27385
                     /locus_tag="LP859_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL3029224.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="LP859_001260"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="LP859_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3029225.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="LP859_001261"
     CDS             27973..28209
                     /locus_tag="LP859_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL3029226.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="LP859_001262"
     CDS             28352..30325
                     /locus_tag="LP859_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL3029227.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="LP859_001263"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="LP859_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL3029228.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="LP859_001264"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="LP859_001264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL3029229.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="LP859_001265"
     CDS             33204..34496
                     /locus_tag="LP859_001265"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL3029230.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAFEZB010000026        34522 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFEZB010000026
VERSION     DAFEZB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.026"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(133..306)
                     /locus_tag="LP859_001266"
     CDS             complement(133..306)
                     /locus_tag="LP859_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3029231.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="LP859_001267"
                     /pseudo
     CDS             754..3116
                     /locus_tag="LP859_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="LP859_001268"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="LP859_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL3029232.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="LP859_001269"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="LP859_001269"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL3029233.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="LP859_001270"
     CDS             5704..6489
                     /locus_tag="LP859_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3029234.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="LP859_001271"
     CDS             6502..7353
                     /locus_tag="LP859_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL3029235.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="LP859_001272"
     CDS             complement(7477..7929)
                     /locus_tag="LP859_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029236.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="LP859_001273"
     CDS             complement(8394..8762)
                     /locus_tag="LP859_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL3029237.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="LP859_001274"
     CDS             8912..9394
                     /locus_tag="LP859_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL3029238.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="LP859_001275"
     CDS             9431..10441
                     /locus_tag="LP859_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL3029239.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="LP859_001276"
     CDS             complement(10556..11269)
                     /locus_tag="LP859_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029240.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="LP859_001277"
     CDS             complement(11459..12049)
                     /locus_tag="LP859_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL3029241.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="LP859_001278"
     CDS             12533..12760
                     /locus_tag="LP859_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL3029242.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="LP859_001279"
     CDS             12776..13942
                     /locus_tag="LP859_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL3029243.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="LP859_001280"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="LP859_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL3029244.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="LP859_001281"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="LP859_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL3029245.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="LP859_001282"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="LP859_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL3029246.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="LP859_001283"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="LP859_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL3029247.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="LP859_001284"
     CDS             complement(19155..19820)
                     /locus_tag="LP859_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029248.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="LP859_001285"
     CDS             19796..19969
                     /locus_tag="LP859_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029249.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="LP859_001286"
     CDS             complement(20014..20751)
                     /locus_tag="LP859_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3029250.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="LP859_001287"
     CDS             complement(20753..22255)
                     /locus_tag="LP859_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL3029251.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="LP859_001288"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="LP859_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL3029252.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="LP859_001289"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="LP859_001289"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL3029253.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="LP859_001290"
     CDS             27597..28862
                     /locus_tag="LP859_001290"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL3029254.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="LP859_001291"
     CDS             28866..29597
                     /locus_tag="LP859_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL3029255.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="LP859_001292"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="LP859_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL3029256.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="LP859_001293"
     CDS             30613..31611
                     /locus_tag="LP859_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL3029257.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="LP859_001294"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="LP859_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL3029258.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="LP859_001295"
     CDS             33123..33773
                     /locus_tag="LP859_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029259.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="LP859_001296"
     CDS             33960..>34522
                     /locus_tag="LP859_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL3029260.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11453 a         5832 c         7324 g         9913 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DAFEZB010000027        30975 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFEZB010000027
VERSION     DAFEZB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.027"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            203..1144
                     /locus_tag="LP859_001297"
     CDS             203..1144
                     /locus_tag="LP859_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL3029261.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1213..1944
                     /locus_tag="LP859_001298"
     CDS             1213..1944
                     /locus_tag="LP859_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL3029262.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2088..4268
                     /locus_tag="LP859_001299"
     CDS             2088..4268
                     /locus_tag="LP859_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL3029263.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4288..4800
                     /locus_tag="LP859_001300"
     CDS             4288..4800
                     /locus_tag="LP859_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL3029264.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVLEDVMEKSNGLRQKEKTLMKHKKSDERIE
                     GLWKKVSRGI"
     gene            4773..5534
                     /locus_tag="LP859_001301"
     CDS             4773..5534
                     /locus_tag="LP859_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL3029265.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5665..6999
                     /locus_tag="LP859_001302"
     CDS             5665..6999
                     /locus_tag="LP859_001302"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL3029266.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7306..7791
                     /locus_tag="LP859_001303"
     CDS             7306..7791
                     /locus_tag="LP859_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL3029267.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7784..8467
                     /locus_tag="LP859_001304"
     CDS             7784..8467
                     /locus_tag="LP859_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL3029268.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8593..11238
                     /gene="polA"
                     /locus_tag="LP859_001305"
     CDS             8593..11238
                     /gene="polA"
                     /locus_tag="LP859_001305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL3029269.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11283..12119
                     /gene="mutM"
                     /locus_tag="LP859_001306"
     CDS             11283..12119
                     /gene="mutM"
                     /locus_tag="LP859_001306"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL3029270.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12083..12712
                     /locus_tag="LP859_001307"
     CDS             12083..12712
                     /locus_tag="LP859_001307"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL3029271.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12764..13096
                     /locus_tag="LP859_001308"
     CDS             12764..13096
                     /locus_tag="LP859_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL3029272.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13408..13899
                     /gene="nrdR"
                     /locus_tag="LP859_001309"
     CDS             13408..13899
                     /gene="nrdR"
                     /locus_tag="LP859_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL3029273.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13922..15295
                     /locus_tag="LP859_001310"
     CDS             13922..15295
                     /locus_tag="LP859_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL3029274.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15295..16221
                     /gene="dnaI"
                     /locus_tag="LP859_001311"
     CDS             15295..16221
                     /gene="dnaI"
                     /locus_tag="LP859_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL3029275.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16350..16790
                     /locus_tag="LP859_001312"
     CDS             16350..16790
                     /locus_tag="LP859_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL3029276.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16934..17635
                     /locus_tag="LP859_001313"
     CDS             16934..17635
                     /locus_tag="LP859_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL3029277.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17632..18753
                     /locus_tag="LP859_001314"
     CDS             17632..18753
                     /locus_tag="LP859_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL3029278.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18768..20000
                     /gene="pepT"
                     /locus_tag="LP859_001315"
     CDS             18768..20000
                     /gene="pepT"
                     /locus_tag="LP859_001315"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL3029279.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20132..21040
                     /locus_tag="LP859_001316"
     CDS             20132..21040
                     /locus_tag="LP859_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL3029280.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21075..21347
                     /locus_tag="LP859_001317"
     CDS             21075..21347
                     /locus_tag="LP859_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL3029281.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21358..22404
                     /locus_tag="LP859_001318"
     CDS             21358..22404
                     /locus_tag="LP859_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL3029282.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22655..25264
                     /gene="clpB"
                     /locus_tag="LP859_001319"
     CDS             22655..25264
                     /gene="clpB"
                     /locus_tag="LP859_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL3029283.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25415..26101)
                     /locus_tag="LP859_001320"
     CDS             complement(25415..26101)
                     /locus_tag="LP859_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029284.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26079..26573)
                     /locus_tag="LP859_001321"
     CDS             complement(26079..26573)
                     /locus_tag="LP859_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL3029285.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26593..27813)
                     /locus_tag="LP859_001322"
     CDS             complement(26593..27813)
                     /locus_tag="LP859_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029286.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            27960..29795
                     /gene="lepA"
                     /locus_tag="LP859_001323"
     CDS             27960..29795
                     /gene="lepA"
                     /locus_tag="LP859_001323"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL3029287.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(29889..>30975)
                     /locus_tag="LP859_001324"
     CDS             complement(29889..>30975)
                     /locus_tag="LP859_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3029288.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10313 a         5547 c         6636 g         8479 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg tttttctgtg cgaaacagga
       61 aagcgaagtc tttttaattc aaataataat attatgtaaa caaaaaactt tccatgcgta
      121 attatacaca agcaaataaa tcgtcaaatg cgaacgcctt ctttatactg tttaggaacg
      181 aaaagaagga aggaagaaat acatggatta tcaagtactg ctttattaca attatacaaa
      241 aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc ttttgcaaat ctttggatct
      301 taaaggtcgt attctagtag ctgaagaagg aataaatggt actgtgtctg gaaccgttga
      361 aaacacgact gcctatatga aagcaatgaa agctgatcct cgatttgctg atacgatgtt
      421 caagatcgat ccaagcgaag gacatgcctt taagaagatg tttgtaagat accgtccaga
      481 gcttgtaagt ttaaagctag aagaggacat tgatccaaac caagtgacag gtgcctatct
      541 ttcaccgcaa gaatttaaag aagctatttt agatgaacag acagtggtga ttgatgcacg
      601 gaatgattat gaatatgact tgggacattt tcgaggggct gtacgcccag atatacgcag
      661 ttttcgagag cttccagact ggatccgcga gcacaaagaa gaatttatgg aaaaacgggt
      721 ggtgacctac tgtactggtg gcattcgttg cgagaagttt tctggttggc ttgtccgtga
      781 aggattcaaa gatgtcggac agctgcatgg cggtattgct acttatggta aggatcctga
      841 agtccaagga gaattatggg acggcaagat gtatgtgttt gatgaacgga tcgctgtcga
      901 tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg tttgacggaa caccatgtga
      961 acgatatatc aattgtagca atcctgagtg caatcgtcag attcttgctt ccattgaaaa
     1021 tgaagaaaaa catttaggag catgtagcat agagtgtgcg aaacatccgc gcaatcgcta
     1081 tgttcaagac catggaatct ctccagaaga agcagaactg cggtttacag ctttaattaa
     1141 atagaaaaca atagttttga ttgtcgacca tttccaatat gcgctatact agcgttgagg
     1201 atggtgatcc acatgaaatc attgatttat gctattgggg atgtccatgg caaacatgag
     1261 ctttttgaaa aacttattca ctattatgat ccaaagatcc atcagcttgt tttattaggg
     1321 gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt tattaggaaa gcaattagtc
     1381 gaagaaacag gagccattta cctccgggga aaccatgaag aatatttttt gcaatttctg
     1441 caatcaccgg aggactggta tgcgccttat atgagaaatg gtggaaaaga aacgtttgaa
     1501 agcctgattc acccaggggc atcggaggaa tactccccaa ctgaaatctc gatgatgatc
     1561 caatcgagat attcagaact gattaaattt ttagtccagc gccccttgta ttatgaatgg
     1621 gaaaattatc tttttgtcca tgccggtgtg gatttgacaa agaaagactg gcgtaagaca
     1681 agtctaagag actttatttg gataagggaa ccatttcatg aagggaaaaa caatacaggt
     1741 aagacaattg tatttggaca tacgattaca ccaatgctcc atggagatat gcagacaaca
     1801 gacctctgga ttcaggatca taaaatcgga atggacggtg gggctgtatt cggagggtca
     1861 ttgcatgggg ttgtttttga caaggaaggt attcttcaag atattgaata tcagaacaac
     1921 gaaggacctt ggcatggaat ctgactaatg gaaagaatga aggagctgtg actttgttgc
     1981 ggctcttttt atttttatag agaatattgt ttctttcagt agtttaaccc aggataaaaa
     2041 gtatgataga ataaagaagc aatttttagc agaaaagagg cattttaatg acggaaaatt
     2101 tgaaccagac gatcattcag cttgtacaaa aagaactttc agactatcgt cctaagcagt
     2161 tgactactgt acttaatttg ttaaatgaag gaaacactgt tccttttatc gcgcgttacc
     2221 gaaaagaaat gactggaagt cttgatgaag tccagattcg tgaaatcgaa gagcggtatg
     2281 cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat tcggctaatc gatgaacaag
     2341 ggaaactgac tcccgagtta gaagcagaaa tcacccaagc cgtgaaaatg caacaagtgg
     2401 aagatttgta tcgtccatac aaacaaaaac gacggacaaa agcaacgatt gctaaagaaa
     2461 aaggacttga accactcgcg aagtggctga tgcagcttac agatggagag gtgcaatctg
     2521 aagcagaaaa atatattgac aaagaaaaag aagtctcttc tgccgaagaa gcgttgcacg
     2581 gtgctcatga gattattgct gaacaagtca gcgataatgc aaaatttcgg acttggattc
     2641 ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt caaggatgag caagcagatg
     2701 aaaaaggcgt atatgaaatg tattatgact ttgcagaacc cgtccataaa atggtttctc
     2761 atcgaatatt agctactaat cgtggagaaa aagaagatat tctgaaagta ttctttcaag
     2821 tagatgaagc agcaatttta gcttacttgg atcgccaaat agtcaaaaat cctgcctctc
     2881 cttcttcttc attcgtcaga gaagcctatc aggatagtta taaacgcttt attcagccag
     2941 caatcgagcg agaactgcgc aatgaattga ctgaaaaggc agatgagcaa gcaatcgcta
     3001 ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc actaaaaggt aaagttgtcc
     3061 tgggatttga tccggcttat cgtactggat gtaaactagc agtcgtagat gcgacaggga
     3121 aagttttagc aatcgaagtt atttatcctc ataaacctgc agcgcaagca aaaagagaag
     3181 ctgctggacc agcttttatc caattaatta ataaatatca agtagatatg gtagctatcg
     3241 gaaatggtac agccagtcgg gaatctgaac tttttgtggc tgagcagttg aaatccgccg
     3301 accataaaac ctattacgct atcgtcaacg aagcaggcgc ttctgtttac tccgctagcg
     3361 aaattgcacg aaaagaattt ccgcatctac aagttgaaga aagaagtgct gtttctattg
     3421 cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat cgatcctaaa gctgtcggag
     3481 taggccagta ccaacatgac gtttctcaaa aacgtttggc tgaacaattg gattttgttg
     3541 tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac agcaagcccg cagctcttgc
     3601 agcatatatc aggattgaat aaaacaactg cccaaaacat tgtcatttat cgtgaagaaa
     3661 acggagaatt caccgcacga actcaattga aaaaagtgcc acgcttagga ccaaaagcat
     3721 atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa aaacattttg gacaacacag
     3781 gtatccatcc cgaaagttat tctattgcaa aagaaatatt aacttcagtc caactttcag
     3841 aaaaagagct tggtacagaa gaagcagtag aaaaattgac acgattatcc gtagagaaac
     3901 tggctgaaag cctatcagtc ggagaagaaa cgttagccga tattttagct gggctgacac
     3961 aacctggacg agatatgcgt gatgagatgc cagcgccctt attacgtaca gatgtattga
     4021 gtatggaaga cttgaaacca gggatggaat tgactggaac ggtccgtaat gtcattgact
     4081 tcggcgcatt tgttgatatc ggtgtgaagc aagacggact tgtccatata tcgaaactca
     4141 gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt aggagatatc gtaacagttt
     4201 ggatagaaca agtcgatacg aaaaaaggca gaatcagttt gacgatgctt tccccttatg
     4261 aagagtaaca atgaggagta acaaaaattg acacaagaag aactgcagca gttagtagaa
     4321 gagatatcat taaaaagctt ccagcttcct ttcaagcacc aagcaatgtt caatccacgc
     4381 ttacgtacaa ctggcgggcg ctatcattta aacgatcatc atttagattt taatgcgaag
     4441 ctatttgaag agtcagacga agagacgatt gcaggaatca tcaaacatga actttgccat
     4501 tatcatcttc atctagctgg aagagggtat cagcataaag atatggattt caagtatctt
     4561 ttgaaaaaaa caggagggtt gcgctatgca ccagccttga cgaagaaaca gataaagatg
     4621 gagtattatc gatgccaaga ttgttctgcc acgatctgcc gcaagagaag aatcgatgta
     4681 ctggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     4741 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     4801 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     4861 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     4921 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     4981 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5041 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5101 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5161 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5221 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5281 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5341 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5401 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5461 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5521 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5581 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     5641 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     5701 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     5761 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     5821 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     5881 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     5941 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6001 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6061 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6121 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6181 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6241 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6301 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6361 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6421 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6481 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6541 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6601 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     6661 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     6721 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     6781 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     6841 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     6901 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     6961 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7021 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7081 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7141 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7201 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7261 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7321 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7381 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7441 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7501 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7561 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7621 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     7681 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     7741 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     7801 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     7861 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     7921 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     7981 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8041 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8101 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8161 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8221 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8281 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8341 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8401 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8461 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8521 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8581 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     8641 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     8701 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     8761 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     8821 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     8881 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     8941 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9001 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9061 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9121 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9181 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9241 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9301 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9361 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9421 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9481 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9541 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9601 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
     9661 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
     9721 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
     9781 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
     9841 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
     9901 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
     9961 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagataa aaaccaagaa
    10021 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10081 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10141 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10201 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10261 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10321 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10381 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10441 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10501 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10561 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10621 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    10681 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    10741 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    10801 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    10861 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    10921 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    10981 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11041 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11101 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11161 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11221 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11281 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11341 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11401 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11461 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11521 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11581 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    11641 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    11701 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    11761 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    11821 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    11881 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    11941 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12001 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12061 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12121 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12181 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12241 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12301 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12361 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12421 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12481 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12541 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12601 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    12661 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    12721 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    12781 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    12841 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    12901 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    12961 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13021 tgaccacatt tccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13081 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13141 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13201 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13261 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13321 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13381 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13441 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13501 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13561 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13621 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    13681 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    13741 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    13801 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    13861 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    13921 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    13981 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14041 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14101 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14161 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14221 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14281 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14341 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14401 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14461 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14521 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14581 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    14641 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    14701 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    14761 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    14821 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    14881 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    14941 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15001 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15061 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15121 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15181 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15241 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15301 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15361 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15421 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15481 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15541 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15601 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    15661 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    15721 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    15781 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    15841 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    15901 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    15961 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16021 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16081 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16141 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16201 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16261 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16321 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16381 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16441 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16501 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    16561 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16621 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    16681 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    16741 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    16801 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    16861 atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag
    16921 aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt
    16981 tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc
    17041 attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta
    17101 tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct
    17161 tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg
    17221 tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag
    17281 cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct
    17341 gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata
    17401 tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat
    17461 gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga
    17521 gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg
    17581 tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc
    17641 agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa
    17701 ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg
    17761 acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg
    17821 attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa
    17881 gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac
    17941 atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg
    18001 gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct
    18061 gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt
    18121 gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt
    18181 gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag
    18241 gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct
    18301 tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta
    18361 ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa
    18421 gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg
    18481 aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa
    18541 agagcagatg tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc
    18601 gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt
    18661 atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct
    18721 gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc
    18781 ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa
    18841 cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag
    18901 agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac
    18961 caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag
    19021 attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga
    19081 tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa
    19141 attatgaagg ccacacattg atcacaacag atggcagtac attactggga gcagatgaca
    19201 aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc
    19261 ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata
    19321 aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag
    19381 gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa
    19441 atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt
    19501 ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct
    19561 tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg
    19621 atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt
    19681 tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca
    19741 tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg
    19801 agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct
    19861 cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat
    19921 tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag
    19981 aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa
    20041 tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc
    20101 tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa
    20161 aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga
    20221 aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga
    20281 attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg
    20341 cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt
    20401 acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga
    20461 agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa
    20521 tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag cagtttggat
    20581 cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt
    20641 attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg
    20701 aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga
    20761 cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat
    20821 tttccgattt attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg
    20881 cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac
    20941 tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg
    21001 attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag
    21061 ttggaagaag agtaatggat gtattgttac acaatctttt gtggttttac cttttcattg
    21121 tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct
    21181 ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat
    21241 tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg
    21301 ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg
    21361 tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt
    21421 gccattattt attatttaat caatcaatca tttgcagata tctttgccga attgctgtct
    21481 accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa
    21541 gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg
    21601 ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg
    21661 atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg
    21721 acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg
    21781 gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct
    21841 ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat
    21901 gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa
    21961 tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc
    22021 caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg
    22081 tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc
    22141 atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt
    22201 ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca
    22261 gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt
    22321 gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa
    22381 gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa
    22441 gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta tatacataca
    22501 atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aaccatgtgt
    22561 atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa
    22621 gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac
    22681 gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat
    22741 cgacattgcc catgtatgga aaatcttttt acaacctaat cattttggtc gaaatttcta
    22801 tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga
    22861 gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt
    22921 tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac
    22981 ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa
    23041 taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga
    23101 aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga
    23161 tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat
    23221 ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga
    23281 acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga
    23341 tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc
    23401 tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa
    23461 aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg
    23521 aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga
    23581 attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa
    23641 agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat
    23701 cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga
    23761 caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc
    23821 ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc
    23881 tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc
    23941 tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga
    24001 attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga
    24061 agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga
    24121 agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc
    24181 tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat
    24241 ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat
    24301 ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact
    24361 tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg
    24421 ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc
    24481 aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga
    24541 agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct
    24601 agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt
    24661 cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt
    24721 cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt
    24781 agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct
    24841 ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct
    24901 attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac
    24961 accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca
    25021 acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc
    25081 tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga
    25141 agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt
    25201 aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt
    25261 ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct
    25321 ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc
    25381 ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt
    25441 tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta
    25501 aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc
    25561 cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt
    25621 cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga
    25681 ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa
    25741 tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc
    25801 aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga
    25861 aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga
    25921 cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt
    25981 gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac
    26041 caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca
    26101 tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc
    26161 aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct
    26221 tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag
    26281 ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg
    26341 atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca
    26401 gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct
    26461 tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg
    26521 ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca
    26581 tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc
    26641 tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc
    26701 acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc
    26761 tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc
    26821 attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac
    26881 aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc
    26941 gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat
    27001 atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt
    27061 tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca
    27121 gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc
    27181 atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc
    27241 atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa
    27301 tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa
    27361 ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg
    27421 atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag
    27481 atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc
    27541 cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat
    27601 cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga
    27661 acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg
    27721 gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa
    27781 ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat
    27841 aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta
    27901 aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta
    27961 tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc
    28021 atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga
    28081 cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa
    28141 tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata
    28201 tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc
    28261 ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa
    28321 cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata
    28381 aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg
    28441 gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata
    28501 ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa
    28561 aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga
    28621 tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat
    28681 ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg
    28741 tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg
    28801 atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga
    28861 atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg
    28921 aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc
    28981 aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc
    29041 aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt
    29101 accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg
    29161 aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa
    29221 atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga
    29281 tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg
    29341 tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg
    29401 aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa
    29461 aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa
    29521 tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag
    29581 ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg
    29641 ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag
    29701 ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg
    29761 ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga aaacagtgta
    29821 aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtggt ttttttcggt
    29881 ggcaaaaatc aagaattctt tctaagattt tccatgatat cgaccgtttc atttctcatc
    29941 ttatctgtta catgcgagta agtatccatc gtaattgaaa ttctgctgtg gcccaatcgt
    30001 tcagagattt ctttcatttt tgcgccattt tcgagaagaa gtgtagcatg agtatgtctg
    30061 agagaatgga agttaaaaga gagggagagt gcattcgata tcctcctagt attccatttc
    30121 actacacttg gcgtaactag ctcaccgtct tccttcgtac atactgcatt tgaatcaatg
    30181 tagagctttc catacttcat tcgattttct aattgttgtt tcttatgttt tttcagaatt
    30241 gctagcaagg tttgtccaat aaaaatcgtt cgattggaac tgcttgtctt tggcgtacca
    30301 tatacccatg cgccgtcatt ctttaccatt tgtttctcta cagtaattgt tccatttgaa
    30361 aaatcgacat catcccacgt cagaccacaa acttcgccaa cgcgcattcc cgtataaaat
    30421 ccaatattca aaggaatata gaaaggatgt ccttcaggag taatttctag catatgatca
    30481 aagtcctcaa gagaaatgat ttttagatct tttttagtcg ttggtcgtgc ttcatatttt
    30541 ggtattttta catacagcat aggattttgc ttgattaatc cccaaggata aaccgccata
    30601 ttcagcgcat tcttaaggac agagtgagta atagtcattg ttttcttcga gtaacccttt
    30661 ttaaattcag cattgatgaa attttgtaaa agagcagggg agagatccgt aagttttttc
    30721 tttcctaaat aaccgtttat atgatttttg atggtaaatc ggtagttttc ataggtattg
    30781 tattttagat ttagtttaac gtattcctcc atccaaaaat caaggtattg ttttactcga
    30841 gtatccgtac ctaaaaagta ttgtcctgtt tcgtcaatat ctgataaaac ttttcgtaaa
    30901 gcagcttcgg cctctggtcg ggtgtctccg ccaactttct ccactttttt tcttgagcca
    30961 tcatcattga tatct
//
LOCUS       DAFEZB010000028        30349 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFEZB010000028
VERSION     DAFEZB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.028"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            171..515
                     /locus_tag="LP859_001325"
     CDS             171..515
                     /locus_tag="LP859_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL3029289.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="LP859_001326"
                     /pseudo
     CDS             516..935
                     /locus_tag="LP859_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="LP859_001327"
     CDS             complement(1027..1377)
                     /locus_tag="LP859_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029290.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="LP859_001328"
     CDS             complement(1343..2674)
                     /locus_tag="LP859_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL3029291.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LP859_001329"
     CDS             2929..3495
                     /locus_tag="LP859_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3029292.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LP859_001330"
     tRNA            3640..3727
                     /locus_tag="LP859_001330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="LP859_001331"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="LP859_001331"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL3029293.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="LP859_001332"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="LP859_001332"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL3029294.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="LP859_001333"
     CDS             complement(7450..7896)
                     /locus_tag="LP859_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL3029295.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="LP859_001334"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="LP859_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL3029296.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="LP859_001335"
     CDS             8361..8879
                     /locus_tag="LP859_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3029297.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="LP859_001336"
     CDS             complement(8956..9912)
                     /locus_tag="LP859_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3029298.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="LP859_001337"
     CDS             10078..10884
                     /locus_tag="LP859_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029299.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="LP859_001338"
     CDS             10881..11870
                     /locus_tag="LP859_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL3029300.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="LP859_001339"
     CDS             11867..12871
                     /locus_tag="LP859_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL3029301.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="LP859_001340"
     CDS             13003..13545
                     /locus_tag="LP859_001340"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL3029302.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="LP859_001341"
     CDS             13565..14263
                     /locus_tag="LP859_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL3029303.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="LP859_001342"
     CDS             14284..14496
                     /locus_tag="LP859_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL3029304.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="LP859_001343"
     CDS             14496..15458
                     /locus_tag="LP859_001343"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL3029305.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="LP859_001344"
     CDS             15476..15871
                     /locus_tag="LP859_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL3029306.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="LP859_001345"
     CDS             16034..16399
                     /locus_tag="LP859_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL3029307.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="LP859_001346"
     CDS             16396..18207
                     /locus_tag="LP859_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL3029308.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="LP859_001347"
     CDS             complement(18269..18436)
                     /locus_tag="LP859_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL3029309.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="LP859_001348"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="LP859_001348"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL3029310.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="LP859_001349"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="LP859_001349"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL3029311.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="LP859_001350"
     CDS             21193..22173
                     /locus_tag="LP859_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3029312.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22364..23086
                     /gene="tsaB"
                     /locus_tag="LP859_001351"
     CDS             22364..23086
                     /gene="tsaB"
                     /locus_tag="LP859_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL3029313.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="LP859_001352"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="LP859_001352"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL3029314.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="LP859_001353"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="LP859_001353"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL3029315.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="LP859_001354"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="LP859_001354"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL3029316.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25705..26406)
                     /locus_tag="LP859_001355"
     CDS             complement(25705..26406)
                     /locus_tag="LP859_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3029317.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            26867..28096
                     /gene="arcA"
                     /locus_tag="LP859_001356"
     CDS             26867..28096
                     /gene="arcA"
                     /locus_tag="LP859_001356"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL3029318.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            28187..29206
                     /gene="argF"
                     /locus_tag="LP859_001357"
     CDS             28187..29206
                     /gene="argF"
                     /locus_tag="LP859_001357"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL3029319.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            29321..30268
                     /gene="arcC"
                     /locus_tag="LP859_001358"
     CDS             29321..30268
                     /gene="arcC"
                     /locus_tag="LP859_001358"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL3029320.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         9919 a         5198 c         6318 g         8914 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaaaaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttgttcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 atacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcagcc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatatc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgcttta agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat cttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaatcgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaatgaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctggatgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatttatcag
    25681 ggtcactgct tatttttgtt cgcgttattc tctcatagct ggagtagtag ctgtcttaca
    25741 gaaagtaaaa cgctttttgt cataatctaa cagcccggaa tcacgtagtt gtttgattac
    25801 ttgtccagca gtttcacgtg tcgtgccgct attcactgct aattctttta aggtgatcgg
    25861 ataaggaatg gtgatatgac catcttttgt tggtacgccc aattcttctt tcaaaatgaa
    25921 taaattttgt tttacccggt caaaggcgct agaggtcaat ccgatttgga tacgcaattc
    25981 atgtatttca actactttag aaattttttg tatcatatag atcaactgtt cgggattttt
    26041 tcgtgctaat ttttcatagt aggaaacagg aaggtagaat gtttcaatat cagtcatcgc
    26101 ttgagctgta taagtgtagg tctcttctcc aaacattcct gacataggaa aaaggttatt
    26161 tgtattcaca tagtcatagt agtaataagt tgcactttca tcataacgtt ctaaacgaac
    26221 gagtcctttg gtcaagatat acaaccgttg tctgggatca ccatcgtcga aaaggatctg
    26281 tcctttttta taagaacgaa gaacagtatg ttccttgatc tgttggaact cttcatccga
    26341 gaaatgttca aaatcaggtt gatatctgag ttttgaaaag tgaaaatcga ttttattaac
    26401 acgcattaaa aagtcctctc cttattgttc actatattta ttgtcacttt ttttaaagaa
    26461 tttgtaaagt tttaacatac caacattaga aacaagtttt aatactttac aaatataatt
    26521 tgttattatg taaaaaatat acaatggtat tgaataattt gttttgaagc gaatgatttg
    26581 tgtataaaaa acaacaataa tacatatagt aaacgctaac aacgataata taatttattt
    26641 ttttaattta ttcaaaaaaa ttttttcaat gaataaatat ttttttgaaa gtgtatacag
    26701 aataaaaaat gaataaatta cgcttaaaaa tagaaaaacg tttaattatt taattatcga
    26761 atattaatgt gaggaatgtc tcattttgaa tgagacaaaa ttatgaaagt gattacaaaa
    26821 ggaggatatc ctatacatgt aatcaaaaag aaggaggcaa cacaacatgg ataaacctat
    26881 tcacgttttc tctgaaatag ggaaattaaa gacagtttta ctgaaacggc ctggacaaga
    26941 agtggaaaat ttgacaccag atattatgga tcgtctgttg ttcgatgata ttccatatct
    27001 tccgattgct caagaagaac atgacaactt tgcgaagaca ttgcaaaatg aaggcgtcga
    27061 gactctttac ttggaaaaat tagcggcaga agcgattgac gcaggcaatg tcaaagaaca
    27121 attcttgaat aaaatgttgg atgaatctca tattgcatcg aatgctgtaa gagagggtct
    27181 tcatgagttc cttctatcaa tggaaacgca agaaatggta gataaaatca tggctggggt
    27241 tcgtacaaaa gatatcgaag tacgttctag cagtctatat gatctgtcag cagacgatga
    27301 ctatccattt tacatggacc caatgccaaa cttgtacttt acacgcgacc cttccgcatc
    27361 aatgggaaat gggatgacag tcaataagat gacttttgaa gcacgtcgtc gtgaatcgat
    27421 gtttacagaa tatattttga aacatcatcc aagatttgca aataaaggaa tcgaagtatg
    27481 gttggatcgt gaaaatccag atcatatcga aggcggagac gaattgatct taagcgataa
    27541 agtagtagct gttgggattt ctcaacgaac taatgcgaaa gctttagaaa aattagcacg
    27601 ccacctattc gcaaaaaatt caggatttga aaaagtgttg gcaatcaaaa ttccgaacaa
    27661 ccgtgcaatg atgcatttgg ataccgtatt tacaatggta gatcacgata aattcactat
    27721 ccatccagca attcaaagta aagacggtaa gatggatgtc tttacaatcg aaccagatgg
    27781 tgatgatatc aagattactc attcagataa cttacacgaa acgatgaaag ctgctcttgg
    27841 attagatgat cttgtactga tcccaactgg taatggagat gagatcgttg cacctcgaga
    27901 acaatggaat gatggatcaa acacattggc aatcgctcca ggtgttgtag tcacttataa
    27961 ccgaaattat gtatcgaacg aattgcttcg tagctatggc atcaaagtgt tagaaatcaa
    28021 ttcaagtgaa ttgtcacgag gtcgtggtgg tccacgctgc atgagccaac cacttgtccg
    28081 tgaagacttg aagtaatttg tttcctgacg gcagtcttac tgtcagctgc cgtcgggatt
    28141 tcctctttaa tcttaaataa aaacaatcta aaggagaatg tttaatatga aagaatcagt
    28201 attccaagga agaagcttat tagcagaaaa agactttaca aaagaagaat tacaatattt
    28261 gatcgatttt tcagaacact tgaaagactt gaaaaaacgt ggtatcccac atcactatct
    28321 agaaggaaaa aatattgcgc ttttatttga aaaaacatct acacgtactc gttctgcatt
    28381 tactactgca gcgatcgatc taggtgcaca tcctgaatat ctaggtgcaa atgatatcca
    28441 attaggtaaa aaagaatcca cagaagatac agctaaagta ctaggacgta tgtttgacgg
    28501 aatcgaattc cgtggattca gtcaaaaaat ggtagaggaa ctagcagaat tctctggtgt
    28561 tccagtttgg aatggcttga ctgatgaatg gcatccaaca caaatgatcg ctgacttttt
    28621 aactatccaa gaaaactttg gaacagtaga aggtatcact gttgcttact gcggagatgg
    28681 tcgaaacaat atggctaact ctttattagt cacaggtgca attttaggag cgaatatgcg
    28741 tatcgttgca ccaaaagaat tacaacctga agaagaaatc gtcaaaatgg cagaaggctt
    28801 tgctgaaaaa tccggtgcac aattgatgat taccgacgat gttgataaag gtgtagacgg
    28861 agcagacgtc ctttattctg atgtatgggt atcaatgggt gaagaagata aattcgaaga
    28921 acgtatcaaa ttgttgaaac cttaccaaat caatatggaa atggtagaaa aaacacacaa
    28981 cacagatcgt ttgatcttct tgcactgctt gccagctttc catgatacaa acactgttta
    29041 cggtgaacaa atgaaagaac gctttggcat cactgaaatg gaagtaacag atgaagtctt
    29101 ccgcagcaaa tatgctcgtc aatttgacca agcagaaaat cgtatgcact caatcaaagc
    29161 gatcatggca gcaacattag gtaacttgtt tatccctcgc gtttaagcat aaccacattc
    29221 atctcacgaa aaaacgagtc agcattgagg gaaaatcaca aaacgctggc tcgttttgtt
    29281 atctagaaaa ttcggtagaa ctgaagaaag gacgaatgaa atggccaaac gaaaagtagt
    29341 tgtcgcttta gggggcaatg ccatcttgtc tacggatgca agtgcaaaag cccaacaaga
    29401 agcgttaatg gaaacagcaa agtatttagt gaaatttatc gaacaaggag atgaattgat
    29461 catctcccac ggaaatggtc cgcaagtcgg taacctattg attcagcagc aagctgctga
    29521 ttctgagaaa acaccggcaa tgcctttaga tacatgtgtt gcaatgacag aaggatctat
    29581 cggttactgg ttacaaaacg ccatgggcga agtactgaag gaaaaaggga tcgataaaga
    29641 tgtggtttct ttagtcactc aagttattgt ggatgaaaat gatccttcat tcaaaaatcc
    29701 aagtaaacca gtcggtccat tctacacgga agaagaagcc aaagaacaaa tgaatgctga
    29761 ctccactgtg acattcaaag aagatgctgg acgtggatgg agaaaagtag tcgcttcccc
    29821 taaacccatc tcgattaaag aagcacgtgt catcgaaacg ctggtcgatc aaggagttat
    29881 cacagtctct gttggtggcg gtgggattcc tgttgtagaa acagctactg gattagaagg
    29941 acgagaagcc gtaatcgata aagattttgc ttcagaaaaa ttagccgaaa tcatcgatgc
    30001 ggatttgctg atcgtattga caggagtaga caatgtgtat gtcaattacc aaaaaccaga
    30061 tcaaaagaaa ttagaaactg ttactgtttc agaaatgaaa caatacattg atgaaaagca
    30121 attcgctcca ggaagcatgc ttccgaaagt agaagcagct attgcatttg ttgaagcaaa
    30181 accaaacgct aaggcaatca tcacttcttt agaaaatatc gagaatcttt tagcttctga
    30241 agaaggaacg attatcgtag ctgattaatt tgttaagtct ttcgtattta cgaaatgatt
    30301 tatagctatc caggtggtat ttcttctcct ttaggccatc tggattttt
//
LOCUS       DAFEZB010000029        29939 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFEZB010000029
VERSION     DAFEZB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.029"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1136
                     /locus_tag="LP859_001359"
     CDS             <1..1136
                     /locus_tag="LP859_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL3029321.1"
                     /translation="NLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKELASQS
                     SLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQTNYN
                     THYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPNTDIL
                     VRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREVQYFS
                     TTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGTMENF
                     IKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLK
                     FLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1577..3217
                     /locus_tag="LP859_001360"
     CDS             1577..3217
                     /locus_tag="LP859_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL3029322.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3371..4693
                     /locus_tag="LP859_001361"
     CDS             3371..4693
                     /locus_tag="LP859_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL3029323.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4755..5186)
                     /locus_tag="LP859_001362"
     CDS             complement(4755..5186)
                     /locus_tag="LP859_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029324.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5250..6086)
                     /locus_tag="LP859_001363"
     CDS             complement(5250..6086)
                     /locus_tag="LP859_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL3029325.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(6276..6578)
                     /locus_tag="LP859_001364"
     CDS             complement(6276..6578)
                     /locus_tag="LP859_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL3029326.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(6604..7035)
                     /gene="ruvX"
                     /locus_tag="LP859_001365"
     CDS             complement(6604..7035)
                     /gene="ruvX"
                     /locus_tag="LP859_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL3029327.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(7032..7298)
                     /locus_tag="LP859_001366"
     CDS             complement(7032..7298)
                     /locus_tag="LP859_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL3029328.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7552..8277)
                     /locus_tag="LP859_001367"
     CDS             complement(7552..8277)
                     /locus_tag="LP859_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL3029329.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8643..8713)
                     /locus_tag="LP859_001368"
     tRNA            complement(8643..8713)
                     /locus_tag="LP859_001368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8679..8681),aa:Gly,seq:ccc)
     gene            8830..9582
                     /locus_tag="LP859_001369"
     CDS             8830..9582
                     /locus_tag="LP859_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL3029330.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9598..10077
                     /locus_tag="LP859_001370"
     CDS             9598..10077
                     /locus_tag="LP859_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL3029331.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10328..11908)
                     /locus_tag="LP859_001371"
     CDS             complement(10328..11908)
                     /locus_tag="LP859_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL3029332.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11901..12644)
                     /locus_tag="LP859_001372"
     CDS             complement(11901..12644)
                     /locus_tag="LP859_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029333.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12820..13596)
                     /locus_tag="LP859_001373"
     CDS             complement(12820..13596)
                     /locus_tag="LP859_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029334.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13844..14680)
                     /locus_tag="LP859_001374"
     CDS             complement(13844..14680)
                     /locus_tag="LP859_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL3029335.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14691..15620)
                     /locus_tag="LP859_001375"
     CDS             complement(14691..15620)
                     /locus_tag="LP859_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL3029336.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15812..16165
                     /locus_tag="LP859_001376"
     CDS             15812..16165
                     /locus_tag="LP859_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL3029337.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16239..16895)
                     /locus_tag="LP859_001377"
     CDS             complement(16239..16895)
                     /locus_tag="LP859_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL3029338.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            17121..19055
                     /locus_tag="LP859_001378"
     CDS             17121..19055
                     /locus_tag="LP859_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3029339.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(19141..20364)
                     /locus_tag="LP859_001379"
     CDS             complement(19141..20364)
                     /locus_tag="LP859_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3029340.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20358..22256)
                     /locus_tag="LP859_001380"
     CDS             complement(20358..22256)
                     /locus_tag="LP859_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL3029341.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22250..23368)
                     /locus_tag="LP859_001381"
     CDS             complement(22250..23368)
                     /locus_tag="LP859_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3029342.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23451..24284)
                     /locus_tag="LP859_001382"
     CDS             complement(23451..24284)
                     /locus_tag="LP859_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3029343.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24277..25194)
                     /locus_tag="LP859_001383"
     CDS             complement(24277..25194)
                     /locus_tag="LP859_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3029344.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25421..26545)
                     /locus_tag="LP859_001384"
     CDS             complement(25421..26545)
                     /locus_tag="LP859_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3029345.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26728..27636
                     /locus_tag="LP859_001385"
     CDS             26728..27636
                     /locus_tag="LP859_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3029346.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27777..29390
                     /locus_tag="LP859_001386"
     CDS             27777..29390
                     /locus_tag="LP859_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3029347.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9317 a         5882 c         5120 g         9620 t
ORIGIN      
        1 acaacttatc catacttgag cagttcatta tgcaattaat tgctggttac tcagctgatt
       61 ctgcagctaa tctcctgaga caagatcctg tgtttaaagc tgttttagat aggaaagaac
      121 tcgcttctca atcttcactt tcccgatttt tagatcgact atctgaggag aatatccatg
      181 aacttcaagc tttgaaccaa gaacttatcg ataaagcacg cctcatccgt aatgatacgg
      241 aattaatcat tgatttagat tcgacccatt cagatacatt tggtcaccaa gaacaaacga
      301 attataatac acactaccaa acctatggtt accatccatt ggtagctttt gacggattga
      361 ctggtgactt cttaaaagct gaactacgtt caggaaatca atatacatca aaaggcgtaa
      421 aggagtttct cacaccttta ttagagcact ataatcactc tctaccaaac actgacatct
      481 tggttcgtgg agacagcggg ttcgctacac ctggtgtgta tgattcgtgt gaatcaaaaa
      541 agagtcatta tgttattcga ctgaagaata atcgtagact aggtcaaata gctgagaaat
      601 cagtacttta tggcgataat caaaagtggg aagaacgaga agttcagtac ttctcgacca
      661 cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg tatacgctca acacgtgaag
      721 cgggcgaact actctttcga catgaattta tcgtgacgaa tctatcagaa aatgtttctc
      781 ctgataccat cttttctatc tatgccaaac gtggcacaat ggagaacttc attaaagaag
      841 cgaaagctgg cttttacttt gacaaaacag acagtcctcg ctttttggag aatcatgtcc
      901 gaatgatgct cagcttgata gcttacaact tagtcaactt cttaagaacg attggctttg
      961 aagaagtcca aaagggaatg accattcatt ctattcgatt gaagtttctg aaagttgctg
     1021 ggaaattagt ccaaacgggt agacgagtct atctcaaatt atctagttat catgtgtatc
     1081 agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc cagtcaatgg atctaatcca
     1141 acaatctaac gattttttta ctgggaagtt atccagggag aagtatgctc aaaatgcctt
     1201 acacccaaaa tttattcctc aatatttttg aacagtgaat gtttgaagta actttttagt
     1261 caaaatcagt tgatagggat attagtgata catatttcaa taaaatgtgc caaagaatta
     1321 aagctatgaa ttattcagga ttctatttat ctatctaaca tatcctttat tcttctactt
     1381 ttctttcttt agcatataag agatacgcat agtaatcaag caaggattgt tgatataaaa
     1441 agaacaaata ccgctatttt gatttctttt gactctatta ctcatataaa taaaatttat
     1501 tatacctgct ctttttgact atatcctcat ctgactccta tacttcgttt gtattaaaac
     1561 ttaggaggaa tcattcatgc ttacagggct aaacttatta aataatcctt ttctaaataa
     1621 aggaacagct tttacaaaag aagaacgggc aaaatacgga atcactggta tgcttccaag
     1681 tactgtccaa acattggaac aacaatctgt ccaagcttac ggtcaatact taagcaaaca
     1741 gaccgatttg gaaaaacgta tcttcttaat gaatctattc aatacgaacc ggacattgtt
     1801 ttataaattg atgggcgaac atttagttga attcatgcct gtcgtttatg atcctgtcgt
     1861 tgcggattca atcgaacaat acaacgaaat tttcttgaaa ccacaagatg ctgtatttct
     1921 ttcaatcgac gaaccagaaa atatcaaagc aagtttgaaa aacgcagcag atggacgcga
     1981 cattcgtttg atcgttgtga cagatgcaga aggaatctta ggtatgggtg actggggtgt
     2041 gaacggtgtt gacatcgcta tcggtaaatt gatggtctac actgccgcgg ctggtatcaa
     2101 tcctgcacaa gtcttgcctg tatcaatcga cgctggtact aataacgaag aattattgaa
     2161 taatccatta tacttaggga atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt
     2221 cattgatcaa ttcgttcaat cagctactga attattccca gaattgttat tacattggga
     2281 agattttgga cgaggaaatg ctgcaacgat cttagaaaaa tacgaagata aaatcactac
     2341 tttcaatgat gatatccaag gcacaggaat cgttgtacta gctggtgtat taggtggatt
     2401 gaatatatca ggtgagcaat tgaaagatca aacgatcttg acatttggtg ctggtactgc
     2461 tggtgtaggt atcgcaaaca tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga
     2521 agaagcacgc caacatttct atcaagtaga taaacagggt ttattgtttg aagatacaaa
     2581 aggattgact cctggtcaaa tcccattcgc tcgtaaacgc tcagaattca ctaatgctga
     2641 tgaattgaca aacttagaag cagttgtaaa agcaatccat ccaacgatca tgatcggtac
     2701 ttctacccaa cctggagcat tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc
     2761 acgtccaatc atcatgcctt tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga
     2821 tttgattgaa tggacggacg gaaaagcttt agttggaaca ggtatccctg ctgctgatgt
     2881 ggaatataac ggtgtaactt accaaatcgg tcaagcaaat aatgcgttga tgtacccagg
     2941 tttagggctt ggtttgattg cttcaacagc tacacgagta aatgctgaaa tcatttctca
     3001 agctagtcga gcattaggcg gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc
     3061 tccagtagca aaaatcactg aattctctca aacgattgct gaaactgtcg caaaatcagt
     3121 tgttgcacaa aaattgaatc gtgaagaaat cacggatatc aaagaagctg ttgaatctgc
     3181 taaatgggtt cctgaatata aatctttaga agattaagat tgttatagta aaagaacata
     3241 cagaaacttt gtatgacgtc taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc
     3301 aactactgct cttttgccgc ttctctttgg cttttttcat acgatacaga ataagaaagg
     3361 atctgtaaaa atggcacaaa aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga
     3421 agggttttgg tcaacaaaaa ttaccggcgt aagtttgccc ctttatttga tcatggtcct
     3481 tgtcctgatc attgcaatgg ctttaggaaa attaccgacg aatatgattg gcccaatcgc
     3541 tgttttggtc attttcggta atttatttca ttttatcgga acaaaaatcc ctatagtcaa
     3601 aagttattta ggtggtggct ccgtttttgc gatctttgcc tctgccgcga ttgctacatt
     3661 tgggatctta cctgagtatg tagtaaaatc cacagaaaac tttgtcagca atatgggctt
     3721 tttagacttt tatattgctg ccttgattac tggtagcatc cttggtatga accgtaatct
     3781 attgatgaaa gcttctgttc gttttattcc cgttgcactt atttcaatgg tcgtctcctt
     3841 ttttgccgta ggactcgtag gtatgcttat cggaaatggt tttgcaaatt ccgttttata
     3901 tatctcattg cccgcaatgg ctggtggtgt tggagccgga gctgtacctt tatcaacgat
     3961 ttatgcaaat gttttaggta cggacgcttc ttctatcatt tcccgcctga ttcctgcgac
     4021 agcaatgaca aatgtcctag ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc
     4081 aatgccaaaa ttcaacggta atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa
     4141 acctagaaat gtaaaacctt ccattccaca attaggtgtc ggtttgatgg tatcattgac
     4201 tttcttcatc ttaggacaaa tctgtaacta ttttgttcct aaagtccatg cttacgcatt
     4261 catgatcatt atcgttgtta tctgtaaaat cgcaaaccta ttaccagaat attacgaaga
     4321 tgcagctatc atgttcaaca acttgattgt caaaaacttg acagcagctg ttcttgccgg
     4381 tatcggtatt gcgttattga atctaaatgt attggcttct gctttgacat ggcaatttgt
     4441 tgtcttatgt ttgacaagtg ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt
     4501 gtttggttta tatccaatcg aatcaactat tactgctggc ttatgcaaca actcgatggg
     4561 tggtacagga aatgtagctg tcttatctgc agctaatcgg atggaactta ttgcttttgc
     4621 tcaaatgggc aatcggcttg gtggtgccat cgttttgatc atctcaggtt tcttgatgca
     4681 attactttct tagtcaaaaa aaatacctga tcatcaagta tcggcttgat gaccaggtat
     4741 tttttgataa aaatttacag gattttcaaa aatctttttt gcattttatc ggaatgataa
     4801 ccgatgcgtt cataaaatcg gtgtgcttct actctgtatt ctgcagaatt aagtcttatt
     4861 gctgatattc ctcgtctttt cgcttcttct acgccagtca tcaaggcctt tccgattcct
     4921 cttttttcag catgtttaga cacagcaagt gccataatat tgaacatcgg atcgctgaaa
     4981 atcgattcat aggactccgc ttgtacatag ccagctgctt ttttagaagt aggatcttca
     5041 tagaccaaga aaaaattttt ttctggctcc ttcaacagtt tcttcatttg tctcaccgta
     5101 acggttaatg gtacatcata tcctaattgt tccgcattga tttctcgtag tgcttctgca
     5161 tctcctaaac gcatttcccg caacatccaa atcaccagcc aatttttatt tttttaaatg
     5221 aattttttta tcacagtata ctcctttttt cacttaaaaa aagacttttc agtgattttg
     5281 acttgcttga atccagatcc ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg
     5341 atcaaaagaa ccacgatgac aatcgcttga aaaataaagg tcactttttt tggagacaaa
     5401 accctgctaa atcttgctcc ccacaaacct ccaataacag cagcggggat aacataaaac
     5461 aacatagata aatcaaagcg cataaagcta cttgttaaag caatcgttac caattttgcc
     5521 agttgagaaa agaaaatcgt agatagagaa tatagtgttg cttcttttat tggcaatgca
     5581 aacatcagca tcaacaaaga aacattgact ggtccaccac caatacctaa aaaactagct
     5641 aaaaagccca gaaccccccc acatataagg taccaagtcc atgacgtcaa atgaaatttt
     5701 ggctgatgat gcttggtgta gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt
     5761 tggatcagct gcacatgctt ctcgttttca aataactgta aaaaaacttc gaatacaatg
     5821 ttgccgacta ctcctccaac aacagctcca ctagataccc aaccgacaat ctgccaattc
     5881 agtgatttcc cgcttgatac ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca
     5941 actgttgagt aaaaagaaat ccctgcaaca gaatgggcgc cgataagatc aagaatcggt
     6001 ttgatcaaca cgcctccacc catcccggaa atagcgccaa ctgtgttcgc cagaacgatc
     6061 accacaaaat aaatcagacc aatcatcttg tatttccttt ctcttgttta tatcttttag
     6121 cgattgttac tcttttcttt tattatacag taaaagaaac aaacgaggct tcgaaagctt
     6181 ttctactttt taaggaaatt tagtgctaaa cgaaaaaacc gggctctact tttcagagag
     6241 tccagctttt cttacacctt atcgattctt ttgttctatt cttcttccgc catgaatgta
     6301 ttgaatactt cttcgatcat atcccattct gcgtctgttt cgatttgttc taactcgcct
     6361 tctgttccat cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct
     6421 gaaacaccag ctggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact
     6481 gttagcaaga tttcgtataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg
     6541 tgtccttcgt gttcgtggga atggttatgt tctgtcataa gattacctct tttcttttta
     6601 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaaaatca tcacagctgc
     6661 taatttatcg atgacttttt ttcgtttcgc tcttgaagta tttgcctgct ctactagcat
     6721 acgttctgcc tgaacagtgg ttagtcgttc atcttgataa tcaaccggca aggagaatag
     6781 ttctttgatc ttctctccat atgacatgga agcttctgcc cttggtccga tggaattgtt
     6841 catattctta ggtaatccaa ctacaaaacg gtctacttcg tactgcgcca ctaattcctt
     6901 caaccgatca aaaccaaact cttcttctgc ctcattgatt cgaatgattt ctactccttg
     6961 agctgtccag ccaaagggat cgctcacagc aacgcctact gtcttagaac cgacatccaa
     7021 gcccattact ctcataaatt cactccatga tttgagaggt agtctttgac aattacttcc
     7081 atgatttcat cgcgttcgtg acggcgaatc aaatttcgtg catcacgata acgaggaatg
     7141 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
     7201 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
     7261 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
     7321 aggtaattcc agcttacctt ctacaccctt attttactcg aagaaaggga aaactacaaa
     7381 caaatattcc aaacttctga ataaattaag aaacacgatt tgtctgattt ttcctttttt
     7441 ctgtggcttt ttcaaaagcc ctctttccat aaagtatacg ctatatttct ttgaaagtat
     7501 gtttttacac aattttttca taaagttcat aaaaaattcg aattgctgct actaattttt
     7561 cgttttcgtt gcttgccctg tctcataatt gatggtgtag ccatcttgaa cattgtagat
     7621 aaagacattg aattcaattt ttttgtcttc aatgctttct gcttccaact gcacacctct
     7681 tgctaccagt tcatttccct cgaaatgagg agtgacacga tagcgtacat gatgatttgt
     7741 ttccttgata tatgccgcaa tatcattttc gtgggcaagc atgtacggag cattcaacga
     7801 acgtgtccct gtcattaagt ttttaatgtt attattttga ccagtcaact ggaaaccaat
     7861 caaatggctg cggttgtata accattgtcc gtcacttaat ttcttttgtt tccaacctgt
     7921 cggatcaata tacaaaggtt ctctcttttc agtaggaaac aagtcttttc ccaacatcgc
     7981 attggcttgt cctactctgt tcaatttgtc tatatttgaa tagctttccc atgtcccttt
     8041 ggaaagacta agatcctctt ttgtaaactc cggctgattc ccattgattt ctatttcttg
     8101 atgttttcca tcatattctg gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa
     8161 aatggttgta atttgttgga agatatctga atctttttgt gtggtggaaa taccgagtgc
     8221 ccctgcaatc acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc
     8281 tttactcctt tactaacagt tccctaaatc ttaacatatg ttcccctata aataaatact
     8341 ggaaagaaat agaaacagta aaaaaataaa accagttttt agacggctta agaatacaaa
     8401 aaaagagacg gattcatcgt ctctactcaa tcacaaaaca ctatttacaa ggtatttatt
     8461 atcattcaaa aaatttttct taatactcaa aactaaacgg cttctgtacg acctcttggt
     8521 aaacagtgtt caaaagcttg catctgcggt aataagtcca accaagtcaa aaacataaga
     8581 agcttttttg cttgactatt cttattactt gatgcagggc agctttttca cagcctcttt
     8641 taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga ttttaccact
     8701 aaactacacc cgcgatacaa aaacagtata cgcctctttc actccttcct caagtttttt
     8761 tattctttac gcttccgata tgttttgtag taaaataaaa ctaatcaatc aattattgga
     8821 gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt actcgctggg
     8881 aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat gagtcgaatt
     8941 gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg tctctttatt
     9001 ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc gattaattta
     9061 gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga gctaacatta
     9121 gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt tcctgtctgg
     9181 ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt tggcggaaag
     9241 atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc ttatctatat
     9301 agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt attgattggc
     9361 atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct aatcattatc
     9421 ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg tgatttgcta
     9481 gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac tgctacgatg
     9541 atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa cacaagtatg
     9601 gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt tgccgttatt
     9661 acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt tggctggatg
     9721 atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt gcttggttct
     9781 ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt
     9841 ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca agcggttcga
     9901 aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc aattatgatt
     9961 gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa catccgtaat
    10021 ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt taaataaacg
    10081 aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc
    10141 tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga gtattaaaaa
    10201 aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata ctgagaaact
    10261 gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt gtcacggccc
    10321 ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc catagagcgg
    10381 aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga taaaacaaag
    10441 ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc atcatagttg
    10501 cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg gtccagttca
    10561 gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag cagccgaata
    10621 aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac agtccgctta
    10681 ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg aacttttctt
    10741 ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt tcttgatcca
    10801 aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc aatgcgatcc
    10861 ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt atttcgcctc
    10921 ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa agaacttgca
    10981 taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc tgatgcacat
    11041 tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca gtcagttgct
    11101 ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga gccgtgatag
    11161 ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca gcatcataga
    11221 atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata gtccccgttt
    11281 gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca agcaagatac
    11341 tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca aaaacaatca
    11401 aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt gagataatga
    11461 tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg actagaagcg
    11521 gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt
    11581 cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa aagatattat
    11641 aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg taataagtaa
    11701 agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa atcagtaaaa
    11761 aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc tttccctttt
    11821 ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga ttaactcgaa
    11881 ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg ccttccagcc
    11941 attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa ttcactaact
    12001 gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca taactgttgt
    12061 gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc atgagccttc
    12121 atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat
    12181 acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca
    12241 tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag cgctgtcaat
    12301 tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga aatcaagtcc
    12361 caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt
    12421 ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa aatcgttccc
    12481 gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc ggcaccatta
    12541 tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc ttttaatgct
    12601 tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt actccttttg
    12661 tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca aaaagagtgc
    12721 ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt ctacaggaaa
    12781 acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt cattttctat
    12841 cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct ctagttgttc
    12901 tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatgt tttcagtatt
    12961 aatgatataa gcttttaaat ctttacataa atactcatat tcgagtgatt ttttatattt
    13021 tttaattgtg ccgtttacga gaatctcttc atctttcaat actaggccta ttttattttt
    13081 ataacctggc atgctcatta gataaaggat ttcatcgtaa tggatatagt gatcgctatt
    13141 accataacgg acagttgtcc accttttttt cgtagcatgt tgaacaggat agttttcttt
    13201 ttctttcaat aagtaacttt ggatgctttt tttatttttt tcataagtga attcttttat
    13261 caaataacca ctaggcgtaa tctttcgatt aataacagtt attgctttat tcggatgaga
    13321 cgttagataa acaattttac acttttcatt cttttctcta agcatttcgc ctagttcgat
    13381 accgctataa taggcgttca aatcgatgtc taggaaaaaa atatctgagg aatagatttc
    13441 attattttta atgaattcaa caaattgtat aacgttcaat ccctgaacga tctcaaattt
    13501 ccaaggactt aataaaattc tggattcttc caaaatttct actagcattt tttcaaaaac
    13561 caaactatca tccaaaataa atatacgcgt taccataaaa agtacctcca ctcgatattt
    13621 tactatcaaa atcatttcat acaacttttc aacatacatg ttaggaataa attgtaattc
    13681 tcttcacaat cttttccaac acctttgaaa ccgatatcaa atactttcag gatgctgctt
    13741 ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa aagccagaca accgacgtcc
    13801 ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt cttttaaaaa gaacgataca
    13861 catactgatt tttcggagca gagactttct cagcgtttgt aacaactaca gatgggattt
    13921 cacgcttgaa tggttgataa tagtctgatt ggatggttcc ttctatttta taccattcat
    13981 catttttaac cgttactcct tccggcatat gcgtcaacaa accaaagact cctgaatctg
    14041 ccacacaatg gataatgccg aatcggaaca agaagatatc tgtattgcta tcgttcggtg
    14101 tttggtaaac aaatccttca tagctgattt ttttgccaat gaattcgcta gggtaattat
    14161 aaattagctc cataacttct aaatagtttt catctgtgat cttgatagtt tgcttatccc
    14221 catacttttc tttcaactca ttcatttggt ctaagtagtc tgatttgttg aaataaatac
    14281 tggtatccgg ctttaagtat tgtgtctgca tctccggatc ccctactgat tctttactta
    14341 ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc gagactaact gttggaaaca
    14401 acgtccccac gattactggc aaagctagca gtacataagc gattccacgt tgataaggtt
    14461 ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga
    14521 cccacaaata cagctgaact aaagccaaaa gagcagataa aatcattgaa agaatagcta
    14581 agtagcgata atggacattg atatattgat cgagtttgcc agaaacttgc aagtacatca
    14641 tcaattcgaa atacccagat aaaatcaaaa accgcagcat attttccctc ctaaataaat
    14701 aatgtgaatc caataacgct aagggcaatc agtccgatca gcgataaaat aaagcgtcct
    14761 gtaaaatatc gtttcatcat cagtagattt ttgatatcga ccattgggcc aaagaccaaa
    14821 aaggcagcta caggtgctgt gccgaacaga cttaacaaag aagatcctat aaacgcatct
    14881 gcttctgagc aaagagacat cgtaaaggcc aataacagca tgatgataat ggctaaaatc
    14941 tttgttgagc cgagcgttag cataattcta gttggtaaat aagtctgcat cgcagctgcg
    15001 attaatccgc caatgatcaa ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc
    15061 aacacatgcc cagtttgatg gatcagctga ttttttggtt cattcttctc actttgttct
    15121 tcttcatgga catgatgatg gtgattttct gctactttag acgtgatttt ttctttcaat
    15181 acagcgtctt tttgaaaata ggcaagccag acaccaatca caagggcaac aaaaaagctg
    15241 cctatcaccc gccagcttgc catctgccaa gaattgccaa atgcgataaa agtggaaaaa
    15301 atcacgattg gattgatgat cggagcagtc agcataaaag caaacgctgt gtgaactggt
    15361 acgccttttt taacaaattg atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag
    15421 aaaaatccta agatcgaacc agtaaaaata gagaagatac gattttcagg aagccatctc
    15481 ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg agataagaca gcctaataat
    15541 acgaaaggta atgcttcaat aacgatcgat aagaaaatcg tactcatctg caaaaccgaa
    15601 tttggaataa aagaaaacat gagtttcctc ctttttattt ctagttcatt cgctttgcaa
    15661 atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa tttttacaca actcttattt
    15721 tttaccaaag cacttcaatt gactttttct gttattccca ttaaattaag gtctcataca
    15781 tatcacttct atcataaaag ggagaataaa aatgaaacaa accactaact tgactgaagt
    15841 tcaagacata ctgcaacagt catccgttgc cgtcatttat ctttcgatgc ctaattgcag
    15901 tgtttgtcac gctgttcgtc cacgactaga ggaacttctc acacattatg atattccggc
    15961 acttcatttg gatgcatttg aaacaccaga agtagctagt aaatttgaag tgctgactgc
    16021 gccagttgtc ctgatttttc atcaaggaaa agaagtcttt cggcaagcac ggttcatcga
    16081 ctttaaaaaa atccgttatc tactagatga actgacagca gaagatgatt ccctttctta
    16141 tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt
    16201 gaaagttttg ccttcatttg ttcaatcgct ttttattttt attctgctgt attttcggaa
    16261 tgcgcatttg cgtcagaatg caagaaatag atcaaggttt gcaattcatt cgtcaaatca
    16321 acattttgga tcaatacatc tgacggagca ttcaatcgag cagcggtaaa gttcaagatt
    16381 cctttgattc ctgcttccgt taactgattg actacttctt gtgctctttc agcgggtatc
    16441 gttaaaattg ctacttcgat ttgttggatc ttgatttgtt ctgacatatc ttccattgga
    16501 taaacgggga taccgtctac gatgcgccca acgatttcct cattcacatc aaaagcacaa
    16561 ctgatacgta tgctattact ttgatggaat ttgtatttta gtaaagcact tcccaagttc
    16621 cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag
    16681 tgcataagat cttctacatc ataaccatac ccacgttttc ctaattcgcc aaaataagaa
    16741 aaatctcgac ggatcgttgc actatctact tgaacggctt cacttaattc agttgaagac
    16801 acttttttct ttccagcatt atttaaaatc cttaaatagc gataatacaa aggcagtctt
    16861 ttagctgtcg cctttggaat aatatgatct ttcacgaaat gacctccacg tattgtctaa
    16921 ttacatcttt gatttctcca tcatagcagt atttatagga aaaaatcaat tccattgctc
    16981 agatattgtg aaattttatt ctttattcat ttatttatca tttctgcaaa tctcgcagta
    17041 taatggaatt tatggtagac tactaattga aaaatagttt tttatttgag gagaattcac
    17101 aaagtagaaa agaggattcc atgatattat tacaagccaa tcagattgct cgccattttg
    17161 gtgcagaagt cttatttgaa aacatacatt tagaaatcag tacaggcgct cgtgtcgctt
    17221 tagttggcag aaatggcgct ggaaaatcga ctttattaaa gatcattgct ggaattgaag
    17281 cacctgatga aggaaagatc gctaaaaata aaacagcgac tctcggatac ctcgctcagg
    17341 acacaggact tgattccaat gaaacagttt ggaacgagat gctgaaagct tttgaacaag
    17401 ttagacaaat ggaagagcgg atgcgcgagc tagagatttt cattagcgaa ggtacgccag
    17461 acacacctgc ttatagttct tatttaaaag aatacgaccg gctgcaacac gatttttcag
    17521 atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg
    17581 attcttcatt ttatgatcag aaaatcgata ctttatcagg cggacagcgc acacgtttag
    17641 ctttagctcg catgcttttg caacaccctg acattttgat tctagacgaa ccaacgaacc
    17701 atttagatat cgagacactt tcctggcttg agaattatct gcaaagctat actggtgcat
    17761 tattgattgt ttcccatgac cgttattttt tagataaagt agtcaatgaa gtctatgaat
    17821 taagcagaag aaaaatgcgc cattacaaag gtaactattc gaaatatcta gatttgaaag
    17881 cagaacagtt agcaagtgaa tggaaggctt atgaaaagca gcaaacagaa atccataaat
    17941 tagaagactt cgttgctcgg aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc
    18001 gcagaaagac attagaaaaa atggaccggc tggatcgtcc tcaaggggat gaaaaacaag
    18061 caaactttct attcagtata gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg
    18121 ccgctatcgg atatgataat gtcaccttat ctgaaccaat caatctagat atccgtcgtc
    18181 aagaagcgat tgctctagta ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa
    18241 tcattcacca gatccctttt atccgtggaa aagaaacgct agggacaaat gtcaccatcg
    18301 gctattacga tcaaggtcaa gctgatctgc atacaaacaa gacgatccta gcagaattgt
    18361 gggatgagca tcctaccact ccagaaaaag agatccgaag tgctttaggt gctttcttat
    18421 tttctggcga agatgtagaa aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg
    18481 ttgctttagc caagttagcg atgaacaaag aaaatttttt gattttggat gaaccaacaa
    18541 accatttaga tattgacaac aaagaagtat tagaaaatgc attgatcgat tatgacggca
    18601 cactgctttt tgtttcccat gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg
    18661 aactctctga aaatggcagc aaagtctatc taggagacta tgattattat ttagagaaaa
    18721 agcaggaaga agaagaaatc gccgaattgt tggcacaaga tgagccagaa tctgctcctg
    18781 ctcccacgaa caaatctgat tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc
    18841 ttcaacgaaa aattactcag gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc
    18901 agttggaaca agaaatggtc gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc
    18961 tgaaccaaga tttagaagct gctagacaag atcaagaaac aaaattgacg gaatgggaag
    19021 aactaagctt ggaattagaa gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt
    19081 catgctgttt tggtttttct ttttttattt tatcaaacaa aaattccata tttacctttt
    19141 tcaccaataa gaagaccaat tactagtcat tctcactaaa gcttctaaat aatagtaatc
    19201 accccaaata caaggtgtat ttaccccatt atttttgggc ttattataaa cagactgtgt
    19261 taaaaagcca tcttcttctt ccgttgtagc atatctttct ttcaattgtc gcataatttt
    19321 ccaaacagca gactggtata agttcttttc tgttttgtct aatagacctg attctattaa
    19381 ttctaataat ccacaaacca tgatcaccat actagatgta tctttttctt ctctgctaga
    19441 atcatcaaag atcaaatccc aataacaaat ggtatcttct ggcaaccgat tcataaaata
    19501 attggctaat attttgctta aatcaaaata tttttgttct ttcaaataac gataacttaa
    19561 tgcaaaaccg taaatccccc atgcttggcc tctagcccaa caggaattat cactataacc
    19621 ttgatgcgta cttccatatc ttggttttcc atctttgaca tccatataaa atgtatgata
    19681 agttgaacca tcttctctta ctaaataatc tgctgcattt tgaatatgag cctgagcagc
    19741 ttttttatat ttttgatttc cagtttcttc actcgcccaa tacaataatg gaagattcat
    19801 gcaacaatcc atgatcattc taccttgttg tgaaggatca ctaggatctc cccaagcttg
    19861 tataactcct gccttctccc aaaaacgctg aagtaatgtt tctgcagcat caattgaagc
    19921 ttccttcatt tttcgatttc ccgttatttt ataatctgct acggttgata aagaatacaa
    19981 aaaaccaaga tcgtgatgat tagtgataat ttgacagtca atcctgtgga taaaatctat
    20041 atctgctttt tcagctgttt gttgaaataa aggatcaccc gtcttctcaa ataacaacca
    20101 attaattcct gtccaaaatc cttctgtcca accaatattg tctcttaatg gataacgtcc
    20161 ttttatagga cactcgcttg ggaattgata ctgactgtta ctgatacgtt tgattaacct
    20221 ctccgtgtgt ctgcgacaac gctcgatctc attattccat tctttttcca ttaattcttt
    20281 ataacattca tttatccatt tttctggatg tattatctct tcaaacatca ctttatcaag
    20341 catttcccca ccagccttta gcataatttt agtctttttt tattgttttt attttggata
    20401 gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat tgttccatac ttctaaacta
    20461 taatcccgat cttgatgagc aaccactcct gttccgagcc aatgggttcc ttttccaaat
    20521 gtaccctcta atccaggaat aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa
    20581 cccataatag ctgtggattt tcgtttaata gtatcgtcac ttaaacagat aatacctgaa
    20641 aagccagctt cactcaccct atagttttcc ttgtcttctt ctatgattat tccttctttc
    20701 tcaggagaac attcaatagc aaaccctgtc tcatatagaa catattctgt atctaattct
    20761 actttgtgta cgcgaatctg ccagccttca aaaggaataa gccaagttct gatttttgcc
    20821 tgtaaagtag gttcccatgt agaggcaagt acctctttct tacatacaat gtctcgtaca
    20881 ttatttcgaa cgatgaactg tccataggtt ttgatgctct cattcttaaa actggtcgtg
    20941 atatatgtgt ccgctcgctg aatccctagc attgaatcca ttgccatttc tgctttcaat
    21001 tgattcgctc gcggtatcga aaaaccaaat tgactagaat aagcaaattt taaatatttt
    21061 tcattggtca atgtatggaa atttggccct aattgtcccg cattcaaaaa aaatgtatgt
    21121 ccattatcat gttgtatgag caaattggct tctgaaagaa gtttagttcc ttcacgaata
    21181 ggcataggca tttctttagc cgtccaatac tcatggtctt caggtagcgc caacaataaa
    21241 aaaatcttat tcagccataa tggagataac gtactattat agggttcagt caatgtcaat
    21301 tgagggtagg tataaccaat attcaggata cctttttcat caaatatttc tttttgcatc
    21361 caccatctta tattccgatt gactaacccc ttgatttcac ctaatttaaa aggcaaaaga
    21421 tcggcccaca acatagctga ccaaaagctt gaaactgcaa aacgataaat catgcttcga
    21481 ccaaaaggca catttgcacc atcttccgca aaaaaatgaa tatagtctct agaaaaatca
    21541 atagctcggt tcttatatat ctggctaatg ggcttttctg gttctagtac gctataaatc
    21601 aaaccataat aatgaaatgc aaaaggatta taataatctt ggcgacctct tgaactgtct
    21661 cgataccagc cattttgaag ataacaattt tctatattat caaatgcttc tttcagtgct
    21721 gattctttaa ctggataacc aaatttttgg ataattgttc cgacaattat cttaaaaaat
    21781 tgccaattac catctgcaca ttcatattca aaaatttgcg agaaccaatg aataatctgg
    21841 cttttttctc ctgtatctaa ttttttccat aataattgtt catgatgata taacgctaat
    21901 aacattgcgg gcatttctac catacgctgg tctctttcat gtatttctcc ccaataatcg
    21961 ggatgggaag gatctgttcc agcttttatt cctgatagaa ttaatttcaa ccatcgttca
    22021 tctaattggt caattgcggg ccccgctccc cataaaaccc gagagaatcc ttccattcct
    22081 gcaattttat ttccatatcc taccccttga tgtatataat caatttgtgc gccacttgct
    22141 cgataaaacg gctggagtgg ttgatttatc tttttaaata agtcaaagta atcctgtctg
    22201 gtccttaata gtggtactgg atgtttcatt gtattttctc ctctttttac taccaaatag
    22261 ctaattccga ctgttctaat cgaagtaagc cttcaataaa aaaataatca gaatagatca
    22321 gtggcacatt ataagtcagt tctctatggt atgcttctgc tccgcctcca atgatcccat
    22381 cttcatccat attccaattt gcatagtgct tttctgtctt ttggagtata ttgatagcag
    22441 cttcatgata catttttgat cgtacagagg aaacatatcc tgctaattcc agaagaccgc
    22501 atgcagcgca tactccagca ctggtatcaa tattcgaatc ttttacagca aaatcaatga
    22561 gaggaatata accagttttt tcgattttct gaataaaatt tactgctatc ttttctgcta
    22621 tagctagata gtacggattt tttgtgtaac gataacttaa cgcgaatcca tataaagccc
    22681 atgcatttcc tctgctccaa caagaattct cattgtatcc ttgcccgcca agtaaccgaa
    22741 tatatttccc tgattctgca tcaaattctg cgatatgccc ggttgatcca tctttcctca
    22801 ctagatactt agctgttgta tctgcatgct gccgagcaat tttagagaac ctcggatcac
    22861 cagtttccct cgaagcccaa tacagtaatg gtaaattcat catactatca ataatcatcc
    22921 aaccaggttt atggtcattc catgctacta aatagttccc tgaaatatta tagcgtccag
    22981 caagtaccgt agctgcatgt aaccctagtt gtcgactatg tttgttttga gactgttgat
    23041 aatgtgcaat agccgatggt aagaataaaa aacctacatc atgatgaacg tctgaaaaat
    23101 ggtacagggc agattctaat aattgttctt gttgtattgc tgcctctttg aacttttctc
    23161 gttttgtctt gtgaaagagc tgccataaaa taccactcca aaaaccattg gtccaccagt
    23221 cgattcctct gtcctttatt acgtctgtat attttccgtt gtaagaaata tatggaattt
    23281 tattccccat tctgtctaat tccgcatcta tcttttcaga agctacattc aaaatttttc
    23341 ttgaccattc cttcgtctgt tcattcatgg atatcccgcc attcttttaa tttttatgaa
    23401 tgtggagttg ctcctagaac gaactagatg gttaacattc gttatttttt ttacttattt
    23461 gcagctccaa tgtcattctc cttaaattta gactgcgccc acacataaat caagaccatt
    23521 gggatcactg aagttgctaa aattagcata attgcgccat aattaattgg ctcacctgta
    23581 ctagaaaccg tcatattttt gataattaag ggaaccgtat atttatcttt atctgaaagc
    23641 atgactaatg gaaatagata ttgattccaa cttcccataa agataagtaa accactagta
    23701 ataatcgtag gtgataaaat cggtacagcg attttcgtaa aaatcaccca ctcattggca
    23761 ccatcgattc tggcagcctc catcagtgct gtaggaaatt gactgctata ttgtcgcatc
    23821 ataaacactg aagttgtact ggcaatcgaa gggatgatca cacctgcata agtattcgat
    23881 aatcctaaac gactcataat gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc
    23941 ctagatagca taactgtatt gaacaacagt ttattcccca taaaacgata ctttgctaat
    24001 gcataacccg ccattgtaga actggctgta gctgaaactg tactgattac agcaataaca
    24061 atcgaattcc aaataatcaa gggataatta tatctcgcca tgatggcttg ccagtttttt
    24121 aatagcatat ccccaaactt gaaattgatt ccgccactta aaatttcttg attactttgc
    24181 gttgaagcta caatcatata taaatatggc atcaaaaata ttaaggcacc aaacacaata
    24241 atgacgtacg taaaaattct gctttgtatc ttttgtttag tcatttttat cacctccgaa
    24301 tttaaattga atagccgata atataacaga tacaaacacc atcgtccagg caatagcaga
    24361 ggcttttcca aaatcaacca tttcaaaact tgttttatat aaattgactc ctacgaccaa
    24421 agctgaattt tctggtccat tggaagtccc aaagaatata tttgggatat tgaaattacc
    24481 caagccattg atagtggcta aaactgtggc cattaaaatg attggcttta gcatggggat
    24541 cgttaataaa aagaattgcc gaaatttcga tgctccatct atttcagcac tttcgtatat
    24601 ctctttagaa atcccttgaa gaccagctaa aaataaaata gtgttatatc ctacactaag
    24661 ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa ggatttgtta accactgtac
    24721 accgttaatt cctagcgcat tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt
    24781 catgaatacg gaagctacag ccaccgttga tgtaatggtt ggaatataat agacagttct
    24841 aaaaaatcct tttcctttta actgtgctga attcaataaa acagcaaagt aaatagctaa
    24901 tagcatcgtt attggaattg atcctgccat gataatcaaa acattcgtgt aagatttaat
    24961 aaacaatgga tctcggaaga tagctagata attgtcaaat ccaacaaatt caaatgagta
    25021 gccaattttc ataaaactaa aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat
    25081 aaagaaaata acaaaaggtg tgaccaatcc ataagcactt tttttatgag acgtatctgt
    25141 tgcttttttc ttgctgttct ttattgtctt gcttggtaac gaagattgca tcatcgttaa
    25201 gtcctcctaa tattttttaa tcaccagtta attttacgcc gtacttatga gcaaaatttt
    25261 ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg
    25321 tagtcaaata agacgtagca tctgaataag tggctggtaa aacagtattt gatgtatttt
    25381 cattgatttt attcaatagt tcatagtatt tttgtcctcc ttagtagtca aactttttct
    25441 cagctgcttc tgtttgatag gccaatgagt tagatgtagc cacgcccagc tccagtgcgt
    25501 tggctaagca ttcttcatct gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat
    25561 ttttggcatc tttagggaca taaaaacttg atcctcctga tactgcacct tttcctggat
    25621 tttcttttga aaaaggaatc agtggagcca tgcgccattt gtcagatgca tcaggaaaat
    25681 tcaaagacat attcgtagca taccagccgc cagcaacaaa cattgcagat tcttgaaatg
    25741 ctttctcagc atcttgtgca ccgtagaaat gtacaatatc tgagtcgatc atttctttga
    25801 ttaactttgc agcatgtttc gcttctttag tattcagacg gatatttcct tcttcatcaa
    25861 gcaaagattg attctgctgg gctagcatgt tgataaacaa ttcttggtcc ccagattctg
    25921 gcaaggcaat tccaaaacta ccagtttttt cttttaatgc ttttgccacc tggatatatt
    25981 cttcccaaga actgatttct tcaaaatcta cacctatttc agcaaatttt tttggttgat
    26041 aataacaaag tgctggggta aaatcactag cccaaggaat aatcgtttca ttactcgttt
    26101 gttgacgcaa aatattcaat ttttgatcat aaaactgatc gccatatttg tcataaaatc
    26161 catattcatc agcactataa aaagcatctt gaaatttttc cagcacacct gctgctgaag
    26221 tgtctgccat ggagataata tccggcatat cttcgtgtgt taccaatttt ggggtgatca
    26281 tttgtactgt tgctgttccc gaagctacat tcacaatttt taagtcaata tttgctccag
    26341 ttttgcggtt atagattttt gcagcatttt caacatagtt tgtcagtttg ccgtttgcga
    26401 catatacttc taatgtagtg gcttttttta tcattggatc tgcgatgttt gctgttactt
    26461 cttcgttagt ttttccacat gcacttaagc tgaataatag ggtaatgttt agcaaagcta
    26521 tcaccaattt gtatcttttt ttcatgtcta tcccttcttt cttttcaagt tcttatataa
    26581 aacgctttca cttatgtata ttaacggaat tagtaacaca aatatttata taaaaaaata
    26641 ttttacttgt cgttttggct tttcattttt gttataatag tcattaatta taaatagaaa
    26701 gctttttata taaaaagaga aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc
    26761 aatcttgtca aaaaaacact aacctattcc atagataccc atcttcattt atctgctgct
    26821 ccctattttc gactactaat aaatgaaagc gctaatttat atgctgaatt tgcacataaa
    26881 tctatagaac tagataatcc ttccctttta ttcattgata aacagcaaga agtccaaatc
    26941 ttttctactc aaaaggaaac acaaactttt atcattatcc agttttattt tgatgagcaa
    27001 ttattagcac atattacggg acaatctttt tctatagatc aagaaataac atctatcatt
    27061 acttttctga aaaatgaaca gttcaaaatt gattatctaa aacatagtaa gccagctatt
    27121 tcctatgatc aggaaatcaa ttcttttttc atactatgcc aacagatgag cattcatttg
    27181 ctttccttat tagttactta ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat
    27241 tatcaacagg aacttgcttt attgacaata ccttcaaatg agaaagtcat tcctaaaaaa
    27301 aagcatattc tttattcaga tgaattagtt gatccaatca tacgatattt agaagaacat
    27361 ctttcagaaa attttttgct tgaacaatta gcccaaattt tttttatcag tccagccagc
    27421 ttaaaaaaac attttagaaa agtgaatgga aaaagtgtta ttagctattt taaagaacta
    27481 aaaatggaaa aagcaaaacg tctcgtgcta gaaaatgaat taacctatac agaaattgca
    27541 gatatattag gatttcattc gatccatcat ttttcttcag cttttaaaaa atatacaggg
    27601 ttatcccctt caaagtatta tctatcctta aaatagaaaa aaatttttag gttttatcca
    27661 caaattattt ctctatattt tctgtctatt caaaataaac aataattttc cggcacaatt
    27721 atttgctgtt ttttaccata acatagtacg atcaacgtag aaaaggagat gtttttatga
    27781 aagttgttgt cattggcggt gtagctggcg gtccttcatt tgccactcgt ttccgtcgat
    27841 tgaatgaagc gcacgaaatc attatctatg aacgcggaga aaatatttct tatgcaagtt
    27901 gtgctttgcc ttattattta ggaggtgtga tcactgaccg tgactcgctg atcgaacgta
    27961 caccagaaat attgaaaaca aaaaacaaca tcgatgtctt tactaaacac gaagtaacag
    28021 caatcgatcc ttctactaag cgattaacag ttaaagacct atccacaaat gaagaaataa
    28081 aaacagatta cgataagttg atcatctctt ctggtgctag accagattat ccggatattc
    28141 ccggagtttt tgaagcagaa aacggctttg tcctccgtag tgtgacggat gcggatcgaa
    28201 tcaaatcgtt ccttgaagaa aaaaatccac aacatgtcgt cattcttggt gcaggtgtta
    28261 tgggtctgga attagctgag aatttcaagc atcgcggctt agacgtgact ttaatcgatc
    28321 aattgccaca agtcgctttc ccttatgatc cagaaattgc taatttagtt tatgacaaat
    28381 tgctgaaaga aggattagcc gttcatttag aaacaagagt tactgagatc cgtgataaag
    28441 gtcgagaaat agtattatca gatggctccg tcctttctgc tgatatgcta atttttgctg
    28501 ttggtgtttc tccgaagaat gaagtggtga aagcaaccgg catacaatta tcagatacag
    28561 gtcagatcat tgttgatgac cagttacaaa ccaatctgcc ggacatctat gcgattggcg
    28621 atattatcga aacgactagt gtagtgactg gtcagccgat ccaaagtatg ctttccagtg
    28681 cggccaatcg tcaaggacat atgctggcag atattttaaa tggtacgcct atgcgctatc
    28741 gcggatatat tggtgcaggt gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa
    28801 tgacagaaca tgaattaaaa gcatcaggca taacaaatta taaaactgtt tttatcactc
    28861 cctttgacca tgcctatttc tatccagaag ctacaagatt aaatctaaag ctgatttttg
    28921 atgcagatag cggtcgtatt ttaggtggac aagcatttgg agaaaaaggt gtcgataaac
    28981 ggatgggaga actttctgta gcaatcaccg gaaacttgac agtctttgat ttgcctgatt
    29041 tggagttgcc ttactctcca ccgtattcta ctacccgtga cccgttgaat atagctggtt
    29101 atgtcgcaat caatcaaatg acgaatatcg tagaaacgat caaagcaagt gatatacccg
    29161 aaaacgattt gaaagaagcg ttctttttag atatacgtga acctaataaa gcaccttctg
    29221 gcagtatctc ggcaacaaaa aatattccaa tgaatgagtt acgagatcga atcaatgaaa
    29281 tcccaaaaga taaaaaagtt tacattactt tcagaagagg attgaatact tatacttctg
    29341 cccgaatctt ggcaggtttg ggtatcaaag cgattttgat tgaagaataa aaaaacgacc
    29401 gcggtgggta gccgcggtct aggagttatt tttacttgag gagtaaaaat gaaaaagtat
    29461 atagggttgt tggttggtat gtatatagaa tagaagataa atgtgaagaa attgtgcatt
    29521 atttcttcac atttattagg ttatagttta agaaagttta aacattgcgc aatttaatta
    29581 ttttagctgt ttcttttcat aaaaaaacat gttgtgactc tgagacaaat catagtccat
    29641 agtgatgtct ctagagtaac tacgcctaat attacgaggc atcatattaa tctatcagca
    29701 ctagtaccta aactagcatc cctttcgaat attttttctt atataataaa gtttgctttc
    29761 actcctctaa tctcaccaaa tcgactggct tgcgttgtgt gatactagga actgattggt
    29821 ttagaacgac ttttctattg taatcatatt tttcgccatc ctcgttgtct tctaatcctt
    29881 caatgaatga tccctaatgt gtgtattaaa taatttaatt tttttgttcc tatttattt
//
LOCUS       DAFEZB010000030        29109 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFEZB010000030
VERSION     DAFEZB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.030"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..1030)
                     /locus_tag="LP859_001387"
     CDS             complement(<1..1030)
                     /locus_tag="LP859_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3029348.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="LP859_001388"
     CDS             complement(1112..1801)
                     /locus_tag="LP859_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL3029349.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LP859_001389"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LP859_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL3029350.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="LP859_001390"
     CDS             complement(2328..2831)
                     /locus_tag="LP859_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL3029351.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LP859_001391"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LP859_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL3029352.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LP859_001392"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LP859_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL3029353.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="LP859_001393"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="LP859_001393"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL3029354.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="LP859_001394"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="LP859_001394"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL3029355.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="LP859_001395"
     CDS             complement(6443..7462)
                     /locus_tag="LP859_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL3029356.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="LP859_001396"
     CDS             complement(7635..8540)
                     /locus_tag="LP859_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3029357.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LP859_001397"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LP859_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL3029358.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LP859_001398"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LP859_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL3029359.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LP859_001399"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LP859_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL3029360.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="LP859_001400"
     CDS             9753..10397
                     /locus_tag="LP859_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3029361.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="LP859_001401"
     CDS             complement(10453..10956)
                     /locus_tag="LP859_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL3029362.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="LP859_001402"
     CDS             11553..12518
                     /locus_tag="LP859_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL3029363.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="LP859_001403"
     CDS             complement(12617..13789)
                     /locus_tag="LP859_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029364.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="LP859_001404"
     CDS             complement(13776..14921)
                     /locus_tag="LP859_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029365.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="LP859_001405"
     CDS             complement(14935..15867)
                     /locus_tag="LP859_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029366.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="LP859_001406"
     CDS             15984..17063
                     /locus_tag="LP859_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL3029367.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="LP859_001407"
     CDS             17060..17689
                     /locus_tag="LP859_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029368.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="LP859_001408"
     CDS             complement(17799..18026)
                     /locus_tag="LP859_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3029369.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="LP859_001409"
     CDS             complement(18129..20717)
                     /locus_tag="LP859_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL3029370.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="LP859_001410"
     CDS             complement(20737..21396)
                     /locus_tag="LP859_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3029371.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LP859_001411"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LP859_001411"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL3029372.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="LP859_001412"
     CDS             complement(22169..23035)
                     /locus_tag="LP859_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3029373.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="LP859_001413"
     CDS             23144..23785
                     /locus_tag="LP859_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL3029374.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LP859_001414"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LP859_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL3029375.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="LP859_001415"
     CDS             complement(25226..26125)
                     /locus_tag="LP859_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL3029376.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="LP859_001416"
     CDS             complement(26127..26924)
                     /locus_tag="LP859_001416"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL3029377.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="LP859_001417"
     CDS             complement(26902..27606)
                     /locus_tag="LP859_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL3029378.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="LP859_001418"
     CDS             27737..28312
                     /locus_tag="LP859_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL3029379.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="LP859_001419"
     CDS             28430..29107
                     /locus_tag="LP859_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL3029380.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6169 c         5232 g         9144 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gcttatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DAFEZB010000031        28758 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFEZB010000031
VERSION     DAFEZB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.031"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            232..663
                     /locus_tag="LP859_001420"
     CDS             232..663
                     /locus_tag="LP859_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL3029381.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISFLNAINS"
     gene            complement(771..1028)
                     /locus_tag="LP859_001421"
     CDS             complement(771..1028)
                     /locus_tag="LP859_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029382.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            1191..2165
                     /gene="bsh"
                     /locus_tag="LP859_001422"
     CDS             1191..2165
                     /gene="bsh"
                     /locus_tag="LP859_001422"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL3029383.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            2895..3212
                     /locus_tag="LP859_001423"
     CDS             2895..3212
                     /locus_tag="LP859_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL3029384.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(3416..3781)
                     /locus_tag="LP859_001424"
     CDS             complement(3416..3781)
                     /locus_tag="LP859_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029385.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            3989..4471
                     /locus_tag="LP859_001425"
     CDS             3989..4471
                     /locus_tag="LP859_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029386.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4668..5558
                     /locus_tag="LP859_001426"
     CDS             4668..5558
                     /locus_tag="LP859_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL3029387.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            5883..8354
                     /locus_tag="LP859_001427"
     CDS             5883..8354
                     /locus_tag="LP859_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3029388.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(8457..10193)
                     /locus_tag="LP859_001428"
     CDS             complement(8457..10193)
                     /locus_tag="LP859_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL3029389.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(10190..10894)
                     /locus_tag="LP859_001429"
     CDS             complement(10190..10894)
                     /locus_tag="LP859_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029390.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            11025..11528
                     /locus_tag="LP859_001430"
     CDS             11025..11528
                     /locus_tag="LP859_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL3029391.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            11488..11826
                     /locus_tag="LP859_001431"
     CDS             11488..11826
                     /locus_tag="LP859_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL3029392.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11847..13265
                     /gene="ffh"
                     /locus_tag="LP859_001432"
     CDS             11847..13265
                     /gene="ffh"
                     /locus_tag="LP859_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL3029393.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            13377..13955
                     /locus_tag="LP859_001433"
     CDS             13377..13955
                     /locus_tag="LP859_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL3029394.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13959..14480
                     /locus_tag="LP859_001434"
     CDS             13959..14480
                     /locus_tag="LP859_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3029395.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(14559..15113)
                     /locus_tag="LP859_001435"
     CDS             complement(14559..15113)
                     /locus_tag="LP859_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL3029396.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            15366..15641
                     /gene="rpsP"
                     /locus_tag="LP859_001436"
     CDS             15366..15641
                     /gene="rpsP"
                     /locus_tag="LP859_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL3029397.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15653..15904
                     /locus_tag="LP859_001437"
     CDS             15653..15904
                     /locus_tag="LP859_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL3029398.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            16029..16820
                     /locus_tag="LP859_001438"
     CDS             16029..16820
                     /locus_tag="LP859_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029399.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16990..17511
                     /gene="rimM"
                     /locus_tag="LP859_001439"
     CDS             16990..17511
                     /gene="rimM"
                     /locus_tag="LP859_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL3029400.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            17501..18262
                     /gene="trmD"
                     /locus_tag="LP859_001440"
     CDS             17501..18262
                     /gene="trmD"
                     /locus_tag="LP859_001440"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL3029401.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            18398..18745
                     /gene="rplS"
                     /locus_tag="LP859_001441"
     CDS             18398..18745
                     /gene="rplS"
                     /locus_tag="LP859_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL3029402.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            19118..19780
                     /locus_tag="LP859_001442"
     CDS             19118..19780
                     /locus_tag="LP859_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029403.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            19859..23086
                     /gene="fss3"
                     /locus_tag="LP859_001443"
     CDS             19859..23086
                     /gene="fss3"
                     /locus_tag="LP859_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL3029404.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            23200..23514
                     /locus_tag="LP859_001444"
     CDS             23200..23514
                     /locus_tag="LP859_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3029405.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            23527..23901
                     /locus_tag="LP859_001445"
     CDS             23527..23901
                     /locus_tag="LP859_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3029406.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            23911..24255
                     /locus_tag="LP859_001446"
     CDS             23911..24255
                     /locus_tag="LP859_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL3029407.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            24326..25678
                     /locus_tag="LP859_001447"
     CDS             24326..25678
                     /locus_tag="LP859_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL3029408.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            25738..26880
                     /locus_tag="LP859_001448"
     CDS             25738..26880
                     /locus_tag="LP859_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBL3029409.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            26905..27159
                     /locus_tag="LP859_001449"
     CDS             26905..27159
                     /locus_tag="LP859_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029410.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            27416..28600
                     /locus_tag="LP859_001450"
     CDS             27416..28600
                     /locus_tag="LP859_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL3029411.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            28597..28734
                     /locus_tag="LP859_001451"
     CDS             28597..28734
                     /locus_tag="LP859_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL3029412.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         9672 a         4901 c         5937 g         8248 t
ORIGIN      
        1 aggggcggta cataaaaatt tattatttag attctataaa catgtgcaac ttatctaaca
       61 acaaagtata tattctacag aagtaaacaa atagcttata ttttaggatg tacacaaatt
      121 gacaagtata ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt
      181 tagaacttac ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga
      241 gagaaaatga tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta
      301 aaaaaagaaa ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc
      361 ttatcagcta gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg
      421 attgtttcaa ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat
      481 tatcaagata aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg
      541 ttatatataa cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc
      601 gttacaaccc ttcaaactga attaaatgat attatctctt ttcttaatgc tataaattca
      661 taataacatt atggacaata aatttaaaat ttattcagac tgtagacaaa ctcctaaatt
      721 tagagtgttg tttgcagtct gacgactcaa aaatgagtcg tataaatcaa ttatagtaat
      781 aagtcattga tttctttaaa ttctttgtca agttcttttt catcatattt ttcatattta
      841 tcagcttcat gaagaacttt tttaatttca tgaatagcct ttcgttcaac taaccatttt
      901 tttaggttat gttttttttc tggattttca gataacgtat caagttcgtc taactcttta
      961 tccagtttat tgacaacaga aacaattttg tttacaactt tttcttcttt gttttctaat
     1021 tttgacattt atcttcacgt cctttttatt tgataattta agtatagaac tttgatatag
     1081 tttatacaaa taaaaacgct tctttgtgta ttctgtgctt ttcttttgga atgaggaaaa
     1141 ctttggagta atatgaacgt ggacagaaaa atatttagga ggaataaact atgtgtacgt
     1201 ctattactta tgtaacaagt gatcattatt ttggaaggaa ttttgattat gaaatatctt
     1261 acaatgaagt agtcactgtt actccaagaa attataagtt gaattttcga aaggtaaatg
     1321 atttggatac tcattatgca atgattggta ttgccgctgg tatagctgac taccctcttt
     1381 attacgatgc gacaaatgaa aaaggattga gtatggctgg gctaaatttt tctgggtatg
     1441 ctgattataa agaaatacaa gaagggaaag acaatgtatc tccttttgaa tttattcctt
     1501 ggattttagg acaatgctca acagtaggag aagctaaaaa attgttaaaa aatatcaatt
     1561 tagcaaatat aaattatagt gatgaacttc ctttatcccc tttacattgg ctattagctg
     1621 ataaagaaaa atcaattgtc attgaaagta tgaaagatgg acttcatata tatgataacc
     1681 ctgtgggcgt tcttaccaat aatccttcat ttgactatca attatttaat ttaaacaatt
     1741 atcgtgtctt atcgagtgaa actcctaaaa ataatttttc aaatcaaata agtttgaatg
     1801 cctatagccg cggtatggga gggataggct tgcctggaga tttatcctca gtatctcgtt
     1861 ttgttaaagc gacttttacg aagctgaatt ctgtatctgg agattcagag tcagaaagta
     1921 ttagtcaatt tttccatatc ttaggttcag tagaacaaca aaaaggtttg tgtgatgttg
     1981 gtgatggaaa atatgaatat acaatttatt cttcttgttg caatgttgac aaaggaatct
     2041 attattatcg aacatatgaa gacagtcaaa ttactgcaat tgatatgaat aaagaagact
     2101 tagatagtca taagttaatt agttatccaa ttatagaaaa acaacaaatt aaatatataa
     2161 attagttaat gtgttgtgat tgattattta atatagatat aatacaaaaa ggcaaagata
     2221 ttttcaaaac attttttctt gatgtgatat ccttgtcttt tttgtaatgt ttttaactta
     2281 ttgcttttgc aaaatttatt tacctgtttt ctggatcaac gatagtgtat attatgctat
     2341 ccttgtttgg attttaggac gtttaaagag gcactttttt acataactta atccatagac
     2401 cttttaagca tgctgtgaga taataataaa gaagagttta aagcgcaccc caaaccactt
     2461 ccccataagt gtattacgct ttaaactctt ttatatttga attcactaag aagcatacca
     2521 tatttttgaa aaaaagtgag aaaaaaggct tataattagg atttagagta attaattaga
     2581 tgtttatttt ataggactga tattatagat tacaaatagt atcgtatttc gcaatcttaa
     2641 aattatcttc ttactatgaa taagataata aaatttgtat ttgtcctttt taggtaactt
     2701 attttaatct atataccttt ctggatcaac gaaagtatac tttatatagt cataacgccg
     2761 atggtaacta tgcactgtaa cgataatgct gctaaaatat ctttttacga aaaacatcac
     2821 aaggaaaaac ttaactatgg tattatctaa acataatttt ttcggtattt aatatttaat
     2881 taggagggaa tattttgctt ttttatagta attttatctt aatagtagcg atattattac
     2941 ttctgaatat atggattttt gataggagta gaaatgcaag tataggtttt agaactaaac
     3001 gaagtctgtc ttctaaaaaa aattgggtat actcacaaac tattttttat ggaggaattg
     3061 ttttaattag tttactttct tcaacacttt attctttgaa tattatagat gtttccacct
     3121 caaattcaat atctattatt ggaatcatta ttgctgcaat tattacacaa ctgtttttag
     3181 tgtttggaga gaagaaaaga agtaaaaaat agaaaaacta gtgatagttt tggtgatagt
     3241 gatattaaaa atcagaaaag atagaaaaag caggagatag ataaaatgct gatatttcag
     3301 cattttttaa gagtataaaa gagaaaaaaa gtaataaatg tatacatgat ataagaaaaa
     3361 catatatgta taaaaagaag aaagtagctt ctctttgtac atatatggtt tgtttttatg
     3421 gatgccgagc ttttttataa gataaggcta ccgcccataa cgacccaagg atttgtggaa
     3481 ctaagtaaat acctgcaaga agcaagatca gctgggtcgg ttttaaagga tgatagaagt
     3541 aacgccattc agtaaaattg gcgcaaaata aataggaagt gacagtagat ataatattgg
     3601 caatgaataa agatataata cgcctgttcc tttgaaaata atatccagca aaaagagata
     3661 aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc agtaaggcga
     3721 aatgcacata aggaacaaaa gataaaagca acggaaggtt tttccagcaa gtttttatca
     3781 tctttcatca ccacctaaca agtgttatta ttttgttagt tttattgtaa attgaatcct
     3841 gcaataatca attacttttt cattatttct agattaaatg aataaactct catgccatta
     3901 tctctaaaaa aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg
     3961 agagaaaaag tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta
     4021 tttatttttt tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt
     4081 acaaggtgcg gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt
     4141 tccagctttg actgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct
     4201 gatcaatcac tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat
     4261 gttttctttg ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa
     4321 ggattcagct tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat
     4381 tgcctcattt attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga
     4441 acgatctgta tctataaaaa ataactattg atggtggatc atcagtaaaa aagcatatga
     4501 gctgcgtaaa atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa
     4561 acgtttacat gatttttaca taacttaact atatttttac agcaaccata cgagggattt
     4621 acagtcgact gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat
     4681 tgtttactct aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata
     4741 cagatggctc tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg
     4801 cggtaggttc gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag
     4861 aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag
     4921 tcgaagctgg agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag
     4981 ttgatgcttc aaaattagtg gatcataaag tagcagtggt cgggatggcg ccaatcgtca
     5041 ataaagatac agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa
     5101 aaatcactaa ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg
     5161 caaacggcag cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg
     5221 tgcagtccca agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag
     5281 gagcaatcag ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg
     5341 acggtgtaga accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg
     5401 aacatatgta cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga
     5461 tgacagaaga aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga
     5521 tggaagtaga acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg
     5581 tagtcgttac tattaaggaa aaatcaacta tgtttcccac atatacattt ttcctttaat
     5641 tgtgaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
     5701 taaacaccgt ttattcgaaa aaaagagcta cgacatagaa ctgtcgtagc tctttttttg
     5761 ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg
     5821 ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc
     5881 acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag
     5941 ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg
     6001 aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc
     6061 aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt
     6121 tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc atagccaata
     6181 caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt
     6241 taaatacagc aaaagtcaat gtcatacggg acgggataga acaaacaata gatatagaag
     6301 accttgtaat agatgacatt gttattttag agactggaca tcaaattcca gcagatgcag
     6361 aggtcgtgga aggaaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa
     6421 taaccaaaga aataggcgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt
     6481 acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca
     6541 agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat
     6601 ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta
     6661 atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac
     6721 ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa
     6781 agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc
     6841 tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa aaagtgatcg
     6901 tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact
     6961 atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa
     7021 aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca
     7081 gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc
     7141 aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg
     7201 tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat
     7261 taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact
     7321 tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca
     7381 atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat
     7441 cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag
     7501 aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg
     7561 atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg
     7621 gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca
     7681 tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct
     7741 tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc
     7801 tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc
     7861 catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga
     7921 atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg
     7981 tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc
     8041 ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca
     8101 gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata
     8161 agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt
     8221 tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta
     8281 aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta
     8341 aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta
     8401 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
     8461 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
     8521 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
     8581 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
     8641 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
     8701 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt
     8761 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
     8821 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
     8881 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
     8941 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
     9001 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
     9061 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
     9121 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
     9181 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
     9241 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
     9301 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
     9361 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     9421 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     9481 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     9541 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     9601 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     9661 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     9721 ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     9781 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     9841 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     9901 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     9961 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
    10021 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
    10081 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
    10141 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgttttg
    10201 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttgga tgttttggat
    10261 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
    10321 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
    10381 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
    10441 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
    10501 gctcagcttc atcttttgat tgcttacgag gttcaatccg tctgaatatt gctttcatac
    10561 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
    10621 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
    10681 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
    10741 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
    10801 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
    10861 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
    10921 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
    10981 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
    11041 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
    11101 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
    11161 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
    11221 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
    11281 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
    11341 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
    11401 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
    11461 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
    11521 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
    11581 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
    11641 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
    11701 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
    11761 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
    11821 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
    11881 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
    11941 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
    12001 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
    12061 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
    12121 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
    12181 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
    12241 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
    12301 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
    12361 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
    12421 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
    12481 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
    12541 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
    12601 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
    12661 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
    12721 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
    12781 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
    12841 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
    12901 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
    12961 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
    13021 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
    13081 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
    13141 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
    13201 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
    13261 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
    13321 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
    13381 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
    13441 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
    13501 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
    13561 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
    13621 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
    13681 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
    13741 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
    13801 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
    13861 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
    13921 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
    13981 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
    14041 acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca
    14101 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
    14161 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
    14221 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
    14281 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
    14341 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
    14401 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
    14461 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
    14521 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
    14581 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
    14641 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
    14701 ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc
    14761 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
    14821 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
    14881 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
    14941 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
    15001 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
    15061 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
    15121 ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa
    15181 acacttactg acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc
    15241 taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt
    15301 tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat
    15361 aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc
    15421 gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta
    15481 cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact
    15541 ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg
    15601 ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga
    15661 tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa
    15721 gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga
    15781 tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta
    15841 cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa
    15901 gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt
    15961 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
    16021 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
    16081 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
    16141 ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct
    16201 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
    16261 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
    16321 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
    16381 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
    16441 aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag
    16501 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
    16561 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
    16621 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
    16681 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
    16741 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
    16801 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
    16861 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
    16921 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
    16981 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
    17041 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
    17101 gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
    17161 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
    17221 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
    17281 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
    17341 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
    17401 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
    17461 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
    17521 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
    17581 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
    17641 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
    17701 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
    17761 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
    17821 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
    17881 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
    17941 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
    18001 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
    18061 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
    18121 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
    18181 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
    18241 aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat
    18301 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa
    18361 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
    18421 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
    18481 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
    18541 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
    18601 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
    18661 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
    18721 tcgtatcaaa gaaatcagaa gataaacacg gaaaacttcc ttggaaatct gaaatttgtg
    18781 aaaagtcctt gtatatcaag ggctttttct tttttctcta tcacagtttt aagccatttt
    18841 tggctgtttt ggaccatttt tcaatcatat tcccttaaaa attgtcctag attgtccgac
    18901 attgactatc agtgtcgtca aaatagggca aaatttagta gtcagcttga cgacaaatat
    18961 aatacgaaaa ataccaaaac atttaccgaa tttagcaact tttgaaccca agtttctcaa
    19021 agggtcagct attagcaaaa aagacaaaac atgtactttt ctactaaaaa gttgatgtga
    19081 taggcttcta tctaaataac tattaggagt ttttatcgtg aagcaaaatg atggacgtat
    19141 catttggaaa aatcaaagtg agttaaaact gattttaaca attaatgaat tcatcgaaaa
    19201 acacggaatt acatctagcc gtcaatacca gaaaaagcta tcagaaaatc caaattctgc
    19261 accaagtatg tggttcatta acaaaaaata tggatcatgg gagaatttat tgattagtat
    19321 aggacgagaa aatactgggt atggaaaatg ggctagaatg tcagaacaag aattattgga
    19381 aatagtagaa gcctttatta agtgcgagaa gataacctct caacgaatgt acgaacaaaa
    19441 atcagtaggg aagaatattc cttcacttag taccattaaa aagatgttag gagatatacg
    19501 tccacttttt aaagaaaaaa atgatggatc tcgatttact gatttcgaac tgctgttaga
    19561 actaaaaaat gaaataatcc gattaaaact acaggatgat ttatcaatga caaagtttcg
    19621 aaaactagtt caatccccaa aactaccatc agttgataca attatgaaaa gaacgaacaa
    19681 aaattgggag gaattgatgg cagagattgg atttgattat cgaagaatca aaatttataa
    19741 gcagagaaac aacctatcca agacaaagaa aactaaatag tatcactcta aatcagacgt
    19801 tcaaaattaa aatttggacg tcttttttat tacccaaaaa cgaaaggaga tttattttat
    19861 gaaaaagcca agatttaaga attggcgttt gctcgcaaca ctgagtctat tatgccaaac
    19921 aattggtgga tcgcttggcc caacgattgc ctttgctgat gaaattactc atccacaaac
    19981 ggtaactgtg gaattggatt tggctcacca gtatgctgtc gaaggtacat ttagcgatgg
    20041 tcgtcccatg tcagaagtta ccgttccgca ctatgcagtt tacaatggtg tgaagcaaga
    20101 tattttctgt attgagccag gggtgccgat ttacaatgag ttcactcctg gttatgagaa
    20161 aaatccattg cctgatatgt cggagaaagc gaaattagtt tccgttttat ggaaggaagc
    20221 tggtacggat gtggatacac atattgtggc ccagaagatg atttggcaag aagttaatgg
    20281 ctacacgctt cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga
    20341 gtcaaaaatc aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac
    20401 agtgaaaacc gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc
    20461 ggagtttgat gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca
    20521 actagtgatt actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc
    20581 agctaataca ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg
    20641 ggcaattgat aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt
    20701 gaagattaaa aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt
    20761 agactttgga aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac
    20821 attggatgag attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc
    20881 ttatacgatt gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt
    20941 gacttcaaaa aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac
    21001 aggaactgat ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa
    21061 agacagtcca accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga
    21121 aacaccaaaa gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa
    21181 agctagtaat ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa
    21241 tcaaaccgtg gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga
    21301 aaccactttg acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt
    21361 taaaggagct gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga
    21421 ggcttttaaa ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac
    21481 tttagatgaa aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca
    21541 agaaaccaaa gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa
    21601 aaaggtggat gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca
    21661 agtcattcgc tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga
    21721 aactggcttc aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac
    21781 agatgccgaa gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg
    21841 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg
    21901 atttcaaaaa attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta
    21961 cactaagagt gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac
    22021 ggtgaccgtt ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt
    22081 gatgctctat gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga
    22141 cggaggcaaa gaattgacag accttgattc taccgagcta gttgataaat tgagctataa
    22201 cttgcatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac
    22261 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc
    22321 aacgttggcg aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga
    22381 acaagttttg gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta
    22441 tgaagcaggt gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt
    22501 caattgtacg gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc
    22561 gcaaaccttt actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga
    22621 tgtactggat ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa
    22681 tacgaccaaa gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt
    22741 cactaaaacc gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt
    22801 taccttcaaa gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga
    22861 tctaaaagaa aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc
    22921 agaacaaccg gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa
    22981 gacattccca caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt
    23041 gatctttgcg actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa
    23101 acaactaaat aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat
    23161 ttttgtatcc aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg
    23221 ccagatatgg ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact
    23281 gaaggatatg ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag
    23341 caacgagccg atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat
    23401 caagatcaac cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag
    23461 aatcgtgggt ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa
    23521 aagaagatga gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta
    23581 cgattttccg cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac
    23641 gaggtaaaag aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt
    23701 agtatcccag caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc
    23761 aatccaatca tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg
    23821 ttcattaagg ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac
    23881 caaaatgaag gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat
    23941 tgtctcgatc aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc
    24001 tatgatgaaa aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgcc
    24061 gaagaagatt ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc
    24121 gaagtaaatg aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa
    24181 atgccggatg ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata
    24241 gcagtaaatg actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt
    24301 tcgattggag gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca
    24361 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
    24421 cgtcttttat tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat
    24481 agaaatcgtt cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg
    24541 aagcacgatg ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg
    24601 acaaaagcta gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag
    24661 tgaaggattt ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc
    24721 caaaatctat taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg
    24781 aaaatatcaa gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga
    24841 actagtagaa aaggaactca aagactccta tgtggagtac actttactgt atgatataat
    24901 tgccaatcga attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa
    24961 aaatcaagta tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg
    25021 tgggaagact tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct
    25081 ttttgttctt gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt
    25141 ctattcccaa aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac
    25201 tcgtagcaag gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct
    25261 tggacttcca ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac
    25321 gacgaaggaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca
    25381 atctggtttc tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg
    25441 gattcgcgat caatttaatt tctgggtggc tttgggtcgt atgagtgaac tgggatattc
    25501 catgatgttt ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta
    25561 tgtagatacg ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata
    25621 tgatttctta agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa
    25681 taatagtatg tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg
    25741 acatttttag atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt
    25801 caccagtgta tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc
    25861 catgatacaa gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt
    25921 caatctctcg gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt
    25981 gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg
    26041 ttcgccgcta ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat
    26101 cacgaaggag gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat
    26161 gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc
    26221 cttatcggac attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct
    26281 ccaacatctg atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg
    26341 gggggacaag tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc
    26401 aaaatcgcat tgccggtaga tgatggaatt tctgtagcgg gtatgttact gggaaatttt
    26461 gaacaaggca atcgggttta tgagataact ggaacagcgc ctactttgtc aacgaaacaa
    26521 ggcggaacga agatcatgct tcggaaaaac gaaacagaat atcaagaggt gaagccaggg
    26581 aacagtgtga acctggcttt tcctaattcc acaagtcgtc gaggccgtct gggaaaacaa
    26641 agtgtgcata ccttattaac aggagaccaa caagcagtcg tcacggatca gtatcaaatt
    26701 cgaaagttaa cacctaggga gtgctggcgg cttcagggct ttccggactg ggcttttgat
    26761 cgagcttctc aagtcaattc agatagtcag ttgtataagc aggctggaaa ttctgtaaca
    26821 gttccggtca tttttgatat tgctagaaga ttaaaggagg taaaaacaga tgatttatga
    26881 tgagttgatt ggggaatcta ctgggtgatt ggcaagattc agtcagatcc ggaattggaa
    26941 gaagaactgt accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccctgatgat
    27001 ccgtatgtaa ttgatgagga aacggatgct cgaatggagc tgaatcaagt catcagtgaa
    27061 ttggaacgaa ttgcggactt tgctaaagaa cctgatatcc gccaatactt gtttgagata
    27121 aaagcagagt tggaaattga gggtattacc gcggagtaac ggccaaataa accactgata
    27181 ggcactgacc gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgtttatg
    27241 gacggcgtta gccggccatg atgtctcacc cccccgtatc taacaggggg gtacaaaaac
    27301 acaaaaaata agcaagcaac gataaggaat ggctgtccta tcaaggataa taagcaattt
    27361 gtgtagatgt taactgattt gaaaaagtta acaccttaaa tttggaggtg agaacttggc
    27421 acaaaggaat ttagattatc ggctattaaa agaccggcgt aatgaatatg gcgtttcaca
    27481 aaataaatta gctacgactt gtggacttag tcgcccgtac ttaaatcaga tagaaaatgg
    27541 tggcgtaacc gcatcgacaa aaaccatgag gaaaattttt gaccaactgg aaagtttcaa
    27601 tcccgagtta cctttaacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
    27661 tgcgaggaaa atcattcaag agattctcca tttgaaattt gattatatgc tccacgaaga
    27721 ttacgccttt tactcctatc aagaacaata tgttatggga gatattgtgg tgatgttgtc
    27781 acatgaagaa gataaaggcg tccttttaga attaaagggt cgtggttgtc ggcagtttga
    27841 aactttttta ctcgcccaaa agcgtagctg gtatgacttt ttcgaagatt gtctaaaagc
    27901 cggtggcgtg atgaaacgct tagacctggc aatcaatgat cgagttggcc tgttagatat
    27961 tcctgattta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtaccttcaa
    28021 aagctatcgt tccggggaac tgttgaaacc tgatgataaa gatggtatgg gcaatacttt
    28081 gtatattggc agtcttaaga gtgaggtcta tttctgtttg tacgaaaaag actatgagca
    28141 atatatcaag ttagggattc ctttagacca gaccgaaacg aaaaatcgtt ttgaaatccg
    28201 actgaaaaat gatcgagctt atcatgcaat tcaagatttg ttaaaaggtc gcagtattga
    28261 aagtaccaca ttttccatta ttaaccggta cttgcgattt gcggataagg tggaaggcaa
    28321 aaggcgaact aactggccct tgaatgaaca atgggggcgc ttcattggac ggaatcagaa
    28381 ggaaattcaa ttaacttctg aaccgaagcc ttataccatt gaacgaacgt tgaactggct
    28441 tggacgacaa gtcgccccta cgtggaagat ggccaaggag ttggatcggt tgaatcagac
    28501 aacgtacatt caggatatgg taaataatac tcgactatca gaccggcaca aaaagatttt
    28561 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtgatggaa
    28621 attgtgaaag atattttatt ggtgtttggt ggcagtatgc ttggtgttac tgtgatgtgc
    28681 ttgatgcatg caagtgcagc ggcggaccga gcaatggaaa agaaaggaaa gtgatgaaat
    28741 gaatttcggt caaaattt
//
LOCUS       DAFEZB010000032        27931 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFEZB010000032
VERSION     DAFEZB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.032"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(119..940)
                     /locus_tag="LP859_001452"
     CDS             complement(119..940)
                     /locus_tag="LP859_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL3029413.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1037..1315)
                     /locus_tag="LP859_001453"
     CDS             complement(1037..1315)
                     /locus_tag="LP859_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029414.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1583..3592
                     /gene="metG"
                     /locus_tag="LP859_001454"
     CDS             1583..3592
                     /gene="metG"
                     /locus_tag="LP859_001454"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL3029415.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4071..4658)
                     /locus_tag="LP859_001455"
     CDS             complement(4071..4658)
                     /locus_tag="LP859_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029416.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            4833..6155
                     /locus_tag="LP859_001456"
     CDS             4833..6155
                     /locus_tag="LP859_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3029417.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6310..7086
                     /locus_tag="LP859_001457"
     CDS             6310..7086
                     /locus_tag="LP859_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL3029418.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7079..7648
                     /gene="rnmV"
                     /locus_tag="LP859_001458"
     CDS             7079..7648
                     /gene="rnmV"
                     /locus_tag="LP859_001458"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL3029419.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7641..8525
                     /gene="rsmA"
                     /locus_tag="LP859_001459"
     CDS             7641..8525
                     /gene="rsmA"
                     /locus_tag="LP859_001459"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL3029420.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            8976..9605
                     /locus_tag="LP859_001460"
     CDS             8976..9605
                     /locus_tag="LP859_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029421.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(9729..10838)
                     /gene="ugpC"
                     /locus_tag="LP859_001461"
     CDS             complement(9729..10838)
                     /gene="ugpC"
                     /locus_tag="LP859_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL3029422.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11047..11469
                     /gene="mgsA"
                     /locus_tag="LP859_001462"
     CDS             11047..11469
                     /gene="mgsA"
                     /locus_tag="LP859_001462"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL3029423.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            11792..12328
                     /locus_tag="LP859_001463"
     CDS             11792..12328
                     /locus_tag="LP859_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL3029424.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12368..14131)
                     /locus_tag="LP859_001464"
     CDS             complement(12368..14131)
                     /locus_tag="LP859_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029425.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14134..15849)
                     /locus_tag="LP859_001465"
     CDS             complement(14134..15849)
                     /locus_tag="LP859_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029426.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(16628..16852)
                     /locus_tag="LP859_001466"
     CDS             complement(16628..16852)
                     /locus_tag="LP859_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029427.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            17043..19820
                     /locus_tag="LP859_001467"
     CDS             17043..19820
                     /locus_tag="LP859_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3029428.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            19901..20320
                     /locus_tag="LP859_001468"
     CDS             19901..20320
                     /locus_tag="LP859_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL3029429.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            20332..20802
                     /locus_tag="LP859_001469"
     CDS             20332..20802
                     /locus_tag="LP859_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029430.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            20819..21598
                     /locus_tag="LP859_001470"
     CDS             20819..21598
                     /locus_tag="LP859_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3029431.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            21585..22406
                     /locus_tag="LP859_001471"
     CDS             21585..22406
                     /locus_tag="LP859_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3029432.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            22423..23472
                     /locus_tag="LP859_001472"
     CDS             22423..23472
                     /locus_tag="LP859_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3029433.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            23485..24489
                     /locus_tag="LP859_001473"
     CDS             23485..24489
                     /locus_tag="LP859_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3029434.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            25122..26141
                     /locus_tag="LP859_001474"
     CDS             25122..26141
                     /locus_tag="LP859_001474"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL3029435.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            26184..27065
                     /gene="galU"
                     /locus_tag="LP859_001475"
     CDS             26184..27065
                     /gene="galU"
                     /locus_tag="LP859_001475"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL3029436.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            27365..27742
                     /locus_tag="LP859_001476"
     CDS             27365..27742
                     /locus_tag="LP859_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029437.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT         9117 a         4864 c         5715 g         8235 t
ORIGIN      
        1 ttttttattc agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca
       61 aagagttgtt tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt
      121 attcatggtg ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac
      181 gttcaccgat ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg
      241 ttttataatc gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat
      301 ctttacctaa aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga
      361 atgctttcaa gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc
      421 cagtgactaa atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat
      481 gacgttctag tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt
      541 ctccaataaa ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg
      601 tactgaatgt atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct
      661 ttgtttctct taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca
      721 atgcccatga atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat
      781 cttcttcttt gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt
      841 ctggacgttc ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat
      901 aatataattt ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag
      961 taaaagtact ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa
     1021 aaatctaggt tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga
     1081 aaagcagata aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa
     1141 taacgatata tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca
     1201 aatgcccatg gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat
     1261 gtgctaatga aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg
     1321 ttccagtccc ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa
     1381 aagccgtttt ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat
     1441 ccttttaaag gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga
     1501 aaaaaagcta acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat
     1561 gcgataaagg aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat
     1621 ctattatcca agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc
     1681 aattgctcgt tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga
     1741 acatggtcaa aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt
     1801 agataaaatg gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa
     1861 attcattcgc acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt
     1921 attagaacag ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga
     1981 agaattcttt acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat
     2041 cggtggtaaa gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt
     2101 ccgcatgagc aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat
     2161 ccagccagaa tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga
     2221 tcttgcagtt tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca
     2281 tgtggtgtat gtatggatcg atgcattatc caattatatt actgcactag gatatggctc
     2341 cgaagatgaa agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga
     2401 aatcgttcgc tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct
     2461 acctaaaaaa gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc
     2521 aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg
     2581 ttattatttg cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt
     2641 tgtttcccgt gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat
     2701 tgctatgatc aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc
     2761 atttgatagt gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat
     2821 ggaaaaaatg gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa
     2881 caaatacatt gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga
     2941 attgaatagt gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca
     3001 gccaatcatg acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac
     3061 aatgaatctt gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa
     3121 aggtacacca atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa
     3181 aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga
     3241 acttgtttca acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa
     3301 agtagcagaa gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg
     3361 actagatgct ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc
     3421 tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa
     3481 gatgcgtgga caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt
     3541 acaagtaatc gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa
     3601 agggggtatg acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc
     3661 gtaaattcct tcttattact tgaagccaat catataatac agaaacagag aactatccaa
     3721 aataaatcct agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact
     3781 aataggaatc gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt
     3841 gattcatctg gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt
     3901 tattacatct atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac
     3961 tttatgttca attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa
     4021 tcatcggctt tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt
     4081 ttatggtaac cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca
     4141 aatgggatcg aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc
     4201 tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa
     4261 tgattatccc aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa
     4321 tagatcccga ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag
     4381 aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac
     4441 atcccccaca agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc
     4501 agcaatatta gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg
     4561 aaccaatgcc aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa
     4621 taatctgcta atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa
     4681 aaggtaggtt tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt
     4741 tcactgaaag tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt
     4801 ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca
     4861 atctgtttgg cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca
     4921 aaactgctgc tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt
     4981 tgggtggttt cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc
     5041 cggaaactgg tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt
     5101 tattaggatg ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta
     5161 tctttgcgac acaattgacg aacttattac aattatctac cgattatctt ttgttgattg
     5221 cagtgatcac cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt
     5281 ctgtacaatc agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg
     5341 gattattgac cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg
     5401 ttacttttgt cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct
     5461 ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag
     5521 caattattct tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt
     5581 tgaaaacttt gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag
     5641 acgtcatttt tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat
     5701 acggtgcatt gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag
     5761 aagacgagct acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt
     5821 atgttcctgc ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg
     5881 actttttaac agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg
     5941 gtgtctttat tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt
     6001 atccaatcgt tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga
     6061 tgacgcaaac gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct
     6121 attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa
     6181 gtacgaacta ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct
     6241 attttgattt ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga
     6301 gagataaaca tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat
     6361 attccagaaa cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc
     6421 ttcgatacgc caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac
     6481 agtattatcg gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa
     6541 ttacaagaac aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat
     6601 cattggatgg aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc
     6661 gcaaaagaaa tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat
     6721 caaatcctaa aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt
     6781 gattatgaat gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc
     6841 gtcactttca aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga
     6901 ttacttgtgg aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat
     6961 gaaccaggct atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca
     7021 gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat
     7081 ggataagatg aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct
     7141 gcgggaagta gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat
     7201 cctgatgcaa attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga
     7261 tttttcagga gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc
     7321 ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc
     7381 aagtgatgct gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac
     7441 ggctgtttct gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca
     7501 agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa
     7561 acaattggaa aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat
     7621 gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa
     7681 aagagatttt attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga
     7741 ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg
     7801 tgatcgaagt tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac
     7861 aagtcgtagc atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac
     7921 cctatgacaa tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg
     7981 ttagagaaac atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata
     8041 ttacgacacc gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg
     8101 tcatgatgca aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg
     8161 gttcattatc gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa
     8221 aaacagtatt tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag
     8281 atataccggc agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat
     8341 ttcagcaaag aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc
     8401 agacaaaagc atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg
     8461 gggagacttt gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc
     8521 agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga
     8581 acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt tgggcttatt accgcagatg
     8641 caagcttttg aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac
     8701 aaggaacaat ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga
     8761 tgcaagcttt tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc
     8821 tcctattttt gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat
     8881 ggatttagaa aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac
     8941 atttatgtgt caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac
     9001 taacgatttc tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa
     9061 tcatgattcc accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt
     9121 tttttacacc aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa
     9181 caacgccggt tatcatcgca ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg
     9241 cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta
     9301 attggcgttt ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc
     9361 tagtagtcag catcttttac tttattggta atatgcctga aacatattat tctcaaggat
     9421 atttttatac agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt
     9481 ataatattgc ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta
     9541 cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat
     9601 attagacatg atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa
     9661 gatgagagtt ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag
     9721 ggggcaattt atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg
     9781 ctgatgttga aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact
     9841 tttgagatga actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat
     9901 tcggaaacga caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt
     9961 tcactgtgga tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt
    10021 tcaactaata ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg
    10081 ataacaccat ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg
    10141 gcaacgaaca tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata
    10201 aatccgtctt tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga
    10261 gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg
    10321 gctacacgta atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca
    10381 tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct
    10441 ggttttcgtt tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt
    10501 ttgatgtctg ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca
    10561 gtcatatgag gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt
    10621 gccacgtcat tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt
    10681 cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata
    10741 aattcacggt ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca
    10801 tattttttat atacgttttt cagagccatt tctaccataa tacgttccac actttctttt
    10861 ttagttttat taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt
    10921 gaacaagaat tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca
    10981 taagaaataa aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg
    11041 tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta
    11101 gcaacagcgt ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg
    11161 cagaggatca cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc
    11221 ggggatcagc aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt
    11281 cgagacccgc tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct
    11341 gatgtctatg agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga
    11401 ctggaagccg gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt
    11461 tcattttgat ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat
    11521 aaaagctaaa caaactgata atatgcgata taatacgaac gtattggata atataaacaa
    11581 taatattagt ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa
    11641 agatcccaaa aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt
    11701 tgcctcttgg acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac
    11761 gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat
    11821 gattgctatt atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat
    11881 cgagacccca tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct
    11941 ttttggtcct ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct
    12001 cttctcgaaa tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat
    12061 ctacggattc tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct
    12121 tagtgtcacg ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca
    12181 tgtgccattg ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt
    12241 accgattcaa tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt
    12301 aagaagttta gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg
    12361 cttgttttta ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt
    12421 gaagcagctc atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct
    12481 ggtccgcatt tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca
    12541 tcattgctaa gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag
    12601 cttcatctaa gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt
    12661 ggcgttgtcc atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg
    12721 gcaaacgttc tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt
    12781 cacttgcttc cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag
    12841 tatcttgtaa caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt
    12901 catggtcatc gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa
    12961 caagtgtgga ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag
    13021 cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat
    13081 caaaagagac ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag
    13141 gcttttcttc tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt
    13201 gcagttgtgt cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga
    13261 tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc
    13321 cgccaactaa accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg
    13381 tcaaggaaga agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt
    13441 caaattgctg ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga
    13501 ttcgttcggt cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg
    13561 tttgtgaagc acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa
    13621 cgaccaatgt cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg
    13681 ccactccaga aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat
    13741 catttgtaaa tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca
    13801 atcgattcag atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc
    13861 gattgcttaa cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga
    13921 atagaataag taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat
    13981 cgatactttg accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta
    14041 gtatcacagc tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga
    14101 gcgtctgcaa ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat
    14161 ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt
    14221 accttgatcc atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat
    14281 aataatcact gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg
    14341 ataatccaac gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc
    14401 tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac
    14461 ttttgtatct aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag
    14521 agcttcccaa cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat
    14581 agtcccggta aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc
    14641 cagctgccaa gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata
    14701 gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc
    14761 aaggactgta tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg
    14821 agtataacgg aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt
    14881 agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt
    14941 tcctgaagct tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa
    15001 aagcatttga ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat
    15061 atttactttg atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt
    15121 tgtagcaggt gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc
    15181 atttgtgctt ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac
    15241 acgaacaccg ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac
    15301 tttttggtag agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg
    15361 caataatgct aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga
    15421 cccgatactt aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg
    15481 aagctgattg atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa
    15541 ctcgttgtga gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa
    15601 tccttgtgaa gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac
    15661 agacatagcg accatccata agcccatttc aatgattttt cctcggtcat tcatttttag
    15721 tccattatcg atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag
    15781 aaatttgaag aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa
    15841 ctgaatcata tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc
    15901 acattgtagc ttaggaaatt tagaatgaaa agagaacaag ccttttcata aatgaaagtt
    15961 ttattatgaa gaagctttag aaattgaata gactattttt tttaaaaaaa agaaaatgag
    16021 aaaacatgcc agtctcttca ttcataggaa cgtctatttt acttaatttt tctataaatg
    16081 aattctgcat aaaaaatata tcaagtaaat gtttatagat gaaactctat ttacttcttt
    16141 tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag
    16201 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
    16261 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
    16321 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
    16381 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
    16441 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
    16501 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
    16561 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
    16621 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
    16681 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
    16741 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
    16801 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
    16861 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
    16921 cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt
    16981 tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag
    17041 gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt
    17101 tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg
    17161 taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca
    17221 aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta
    17281 agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg
    17341 cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta
    17401 tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag
    17461 gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc
    17521 atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata
    17581 atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag
    17641 aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg
    17701 tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga
    17761 tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta
    17821 cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa
    17881 tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt
    17941 tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt
    18001 cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag
    18061 tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc
    18121 atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa
    18181 tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc
    18241 agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc
    18301 tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt
    18361 ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag
    18421 agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg
    18481 ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata
    18541 ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc
    18601 actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta
    18661 tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac
    18721 tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag
    18781 taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac
    18841 cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat
    18901 tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt
    18961 tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg
    19021 aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc
    19081 agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt
    19141 gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta
    19201 cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca
    19261 gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata
    19321 tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac
    19381 tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca
    19441 tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga
    19501 aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca
    19561 tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga
    19621 tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag
    19681 aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga
    19741 tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct
    19801 cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt
    19861 gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt
    19921 agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa
    19981 aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac
    20041 agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt
    20101 tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt
    20161 tgccggattg aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga
    20221 gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa
    20281 cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta
    20341 ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca
    20401 ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag
    20461 acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac
    20521 tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa
    20581 tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta
    20641 ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca
    20701 gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa
    20761 gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat
    20821 gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact
    20881 agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg
    20941 tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt
    21001 ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt
    21061 tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac
    21121 gttgacattg atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa
    21181 agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc
    21241 cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag
    21301 caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt
    21361 agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa
    21421 acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt
    21481 gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt
    21541 ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga
    21601 tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa
    21661 tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc
    21721 cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc
    21781 tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa
    21841 tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa
    21901 catctttgat gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat
    21961 tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct
    22021 tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg
    22081 gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt
    22141 tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga
    22201 tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta
    22261 tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc
    22321 tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt
    22381 ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat
    22441 tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca
    22501 agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac
    22561 gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa
    22621 gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct
    22681 gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa
    22741 gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca
    22801 caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct
    22861 gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc
    22921 agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca
    22981 gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca
    23041 cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg
    23101 aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac
    23161 ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta
    23221 aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc
    23281 accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat
    23341 gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca
    23401 gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa
    23461 agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca
    23521 ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg
    23581 gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa
    23641 gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa
    23701 tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt
    23761 accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca
    23821 ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc
    23881 acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg
    23941 tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga acgttattta
    24001 ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa
    24061 aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt
    24121 tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat
    24181 gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta
    24241 tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa
    24301 atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa
    24361 aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat
    24421 gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt
    24481 gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc
    24541 tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta
    24601 tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta
    24661 tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt
    24721 gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc
    24781 aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc
    24841 tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt
    24901 aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag
    24961 tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata
    25021 aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata
    25081 ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag
    25141 ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg
    25201 atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa
    25261 atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg
    25321 cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt
    25381 cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta
    25441 aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag
    25501 aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag
    25561 tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac
    25621 aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag
    25681 aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag
    25741 gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc
    25801 ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact
    25861 tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac
    25921 agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa
    25981 cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa
    26041 caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt
    26101 tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa
    26161 tcgtcgttta ggaggaacag caagtgaagg tcaaaaaagc agtcattcct gccgctgggc
    26221 taggaacacg ttttttgccg gcaacaaaag ccatggcaaa agaaatgctt ccaatcgtgg
    26281 ataaaccaac gatccagttt attgtggaag aagcattgga ttcgggaatc gaagacatct
    26341 tgatcgtaac gggaaaagaa aaaagaccca ttgaagatca ctttgatgca aatatcgaat
    26401 tggaaaacaa tttgcgggaa aaggaaaaaa cagaattact cgctttagtt gaagaaacga
    26461 ctcaagtcaa tcttcacttc atccgtcaat ctcatccaag aggactgggg gatgccgtgc
    26521 ttcaagcaaa agcatttata ggaaatgaac cgtttgtcgt catgctgggg gatgatttga
    26581 tgaaagatga tatcccattg actcaacaat tgatgatgga ttatgaagaa acacaagctt
    26641 ccgctgttgc cgtcatgcgt gtaccagaaa atgaaacatc taaatacgga attattgatc
    26701 ctaaaacaga tagcgaaaag ggtcgttgca gagtaaaagg gtttgtagaa aaacctgaaa
    26761 tgggaaaagc accaagtaat ttagccatta tcggacgtta tctactgact cctaaaatat
    26821 ttgagatatt agaaactcaa gaaccaggag caggaaacga gatacagcta acggatgcac
    26881 ttcagactct taaccagaca gaggcggtct atgcgagaga gtttaaagga aaaagatacg
    26941 acgtaggcga taagttagga tatatgaaga caaatattga atatggactt caacattcgg
    27001 aaattgggac ttcattaagt gcatatatta tccagttgag taaaaagcta agaacagaaa
    27061 gttaactgga aataagtggc ctgctgagaa tgaaaggcta tttgttatta aagcggcagc
    27121 ttgtgcgtca tttgcgcaac tgtcgtttta gttttttgac tctagatcag ttgagtcgtg
    27181 gaaaacgaaa gatagaaaaa gactttcgtg aatagattca gaaaatatta tgctaatggt
    27241 ttaatttttt tttaagttgt ataaaagaaa atgattacat aagagaaatg aaagccaagc
    27301 cagaagattc atagtagaat aaaaaggcga attttttaat tataaaaaaa ggtgtgtgtt
    27361 cactatggga aaattagtcg ctgttgcaca gtcgctgctt gatatactca agtggctgat
    27421 ggaaggaagc aattggatat ttgtgttgcc actattgctt attagtctat ttatcatgtt
    27481 tcgcatggtc agaaaaaaag aaacgtttct gatttttatc tggtatgcag catccgttgg
    27541 ggttgctacc ttgatggttg aattggcgtt aggtagttgg gaagcttgcg tatttgcttt
    27601 attttttgct gtactgtatc taatcattta tatcccatat atgaaaagaa agtctcggat
    27661 aggcagaaaa ggtagacata caactacttt ttctagtaca gattctctcg aacaaagtag
    27721 aagaacgaga agaagacggt aatatttagt aggtatacgc aaaaaaagat aaaataatgg
    27781 ataaactatt gagatgagag atttatttaa ttggtggaaa aaagcttttt tcacttatat
    27841 aaaagaatct tttttaaagg aatgcttgtt gtatcgggat aagccattcc tttttttatg
    27901 ttatttatat atatataaat cataccaatg t
//
LOCUS       DAFEZB010000033        27578 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFEZB010000033
VERSION     DAFEZB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.033"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..333)
                     /locus_tag="LP859_001477"
     CDS             complement(<1..333)
                     /locus_tag="LP859_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029438.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(508..2643)
                     /locus_tag="LP859_001478"
     CDS             complement(508..2643)
                     /locus_tag="LP859_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3029439.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2726..4381)
                     /locus_tag="LP859_001479"
     CDS             complement(2726..4381)
                     /locus_tag="LP859_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3029440.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4383..4697)
                     /locus_tag="LP859_001480"
     CDS             complement(4383..4697)
                     /locus_tag="LP859_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL3029441.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4867..5127
                     /locus_tag="LP859_001481"
     CDS             4867..5127
                     /locus_tag="LP859_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL3029442.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5141..5455
                     /locus_tag="LP859_001482"
     CDS             5141..5455
                     /locus_tag="LP859_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL3029443.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5940..6494)
                     /locus_tag="LP859_001483"
     CDS             complement(5940..6494)
                     /locus_tag="LP859_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL3029444.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6620..8548)
                     /locus_tag="LP859_001484"
     CDS             complement(6620..8548)
                     /locus_tag="LP859_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL3029445.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8854..9570)
                     /locus_tag="LP859_001485"
     CDS             complement(8854..9570)
                     /locus_tag="LP859_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL3029446.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="LP859_001486"
     CDS             complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="LP859_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL3029447.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="LP859_001487"
     CDS             complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="LP859_001487"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL3029448.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13040..14500)
                     /locus_tag="LP859_001488"
     CDS             complement(13040..14500)
                     /locus_tag="LP859_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029449.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14490..15785)
                     /locus_tag="LP859_001489"
     CDS             complement(14490..15785)
                     /locus_tag="LP859_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3029450.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16117..16794)
                     /locus_tag="LP859_001490"
     CDS             complement(16117..16794)
                     /locus_tag="LP859_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL3029451.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16824..17915)
                     /locus_tag="LP859_001491"
     CDS             complement(16824..17915)
                     /locus_tag="LP859_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL3029452.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVERVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18016..18450)
                     /locus_tag="LP859_001492"
     CDS             complement(18016..18450)
                     /locus_tag="LP859_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3029453.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19034..20491)
                     /locus_tag="LP859_001493"
     CDS             complement(19034..20491)
                     /locus_tag="LP859_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3029454.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20488..22383)
                     /locus_tag="LP859_001494"
     CDS             complement(20488..22383)
                     /locus_tag="LP859_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3029455.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22441..24216)
                     /locus_tag="LP859_001495"
     CDS             complement(22441..24216)
                     /locus_tag="LP859_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3029456.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24266..25762)
                     /locus_tag="LP859_001496"
     CDS             complement(24266..25762)
                     /locus_tag="LP859_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3029457.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25956..27578)
                     /locus_tag="LP859_001497"
     CDS             complement(25956..27578)
                     /locus_tag="LP859_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL3029458.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         7812 a         5943 c         4590 g         9233 t
ORIGIN      
        1 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
       61 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
      121 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
      181 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
      241 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
      301 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
      361 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
      421 aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag
      481 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
      541 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
      601 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
      661 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
      721 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
      781 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
      841 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
      901 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
      961 atggtagtca tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
     1021 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
     1081 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
     1141 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
     1201 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
     1261 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
     1321 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
     1381 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
     1441 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
     1501 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
     1561 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
     1621 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
     1681 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
     1741 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
     1801 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
     1861 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
     1921 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
     1981 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
     2041 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
     2101 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
     2161 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
     2221 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
     2281 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
     2341 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
     2401 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
     2461 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
     2521 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
     2581 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
     2641 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
     2701 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
     2761 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
     2821 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
     2881 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
     2941 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
     3001 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
     3061 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
     3121 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
     3181 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
     3241 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
     3301 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
     3361 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
     3421 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
     3481 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
     3541 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
     3601 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
     3661 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
     3721 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
     3781 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
     3841 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
     3901 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
     3961 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
     4021 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
     4081 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
     4141 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
     4201 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
     4261 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
     4321 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
     4381 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
     4441 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
     4501 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
     4561 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
     4621 aacgctggat tttccaaaat cttttccttt aattctggga ctgttatcga ttttattgat
     4681 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
     4741 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
     4801 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
     4861 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
     4921 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
     4981 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
     5041 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
     5101 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
     5161 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
     5221 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
     5281 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
     5341 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
     5401 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
     5461 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
     5521 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
     5581 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
     5641 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
     5701 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
     5761 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
     5821 aagaaataat ttcaaaaaca acttctcctg tttttggttt ggttggctta ttatcgagaa
     5881 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
     5941 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
     6001 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
     6061 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
     6121 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
     6181 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
     6241 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
     6301 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
     6361 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
     6421 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
     6481 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
     6541 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
     6601 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
     6661 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
     6721 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
     6781 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
     6841 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
     6901 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     6961 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     7021 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     7081 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     7141 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     7201 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     7261 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     7321 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     7381 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     7441 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     7501 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     7561 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     7621 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     7681 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     7741 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     7801 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     7861 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     7921 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     7981 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     8041 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     8101 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     8161 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     8221 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     8281 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     8341 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     8401 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     8461 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     8521 gtaattgact aatcatttcg ttgtctatat tctctccttt tcttgaagag atacctgtcc
     8581 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     8641 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     8701 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     8761 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     8821 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     8881 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     8941 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     9001 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     9061 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     9121 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     9181 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     9241 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     9301 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     9361 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     9421 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     9481 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     9541 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     9601 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     9661 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     9721 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     9781 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     9841 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     9901 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     9961 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    10021 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    10081 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    10141 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    10201 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    10261 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    10321 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    10381 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    10441 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    10501 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    10561 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    10621 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    10681 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    10741 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    10801 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    10861 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    10921 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    10981 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    11041 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    11101 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    11161 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    11221 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    11281 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    11341 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    11401 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    11461 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    11521 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    11581 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    11641 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    11701 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    11761 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    11821 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    11881 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    11941 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    12001 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    12061 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    12121 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    12181 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    12241 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    12301 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    12361 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    12421 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    12481 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    12541 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    12601 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    12661 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    12721 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    12781 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    12841 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    12901 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    12961 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    13021 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    13081 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    13141 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    13201 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    13261 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    13321 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    13381 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    13441 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    13501 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    13561 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    13621 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    13681 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    13741 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    13801 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    13861 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    13921 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    13981 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    14041 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    14101 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    14161 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    14221 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    14281 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    14341 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    14401 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    14461 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    14521 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    14581 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
    14641 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    14701 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    14761 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    14821 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    14881 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    14941 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    15001 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    15061 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
    15121 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    15181 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    15241 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    15301 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    15361 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    15421 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    15481 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    15541 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    15601 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    15661 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    15721 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    15781 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    15841 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    15901 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    15961 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    16021 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    16081 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    16141 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    16201 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    16261 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    16321 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    16381 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    16441 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    16501 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    16561 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    16621 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    16681 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    16741 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    16801 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    16861 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    16921 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    16981 gcaagttctg catatttcaa tacccgctct acatcttcgc gattgcggat cccaccgaca
    17041 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    17101 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    17161 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    17221 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    17281 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    17341 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    17401 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    17461 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    17521 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    17581 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    17641 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    17701 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    17761 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    17821 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    17881 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    17941 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    18001 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    18061 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    18121 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    18181 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    18241 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    18301 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    18361 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    18421 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    18481 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    18541 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    18601 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    18661 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    18721 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    18781 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    18841 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    18901 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    18961 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    19021 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    19081 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    19141 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    19201 acgacgtata accaaataaa tctactccat cttcgatagc ttccttcatc tgctcaatat
    19261 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    19321 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    19381 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    19441 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc aaattttctg
    19501 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    19561 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    19621 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    19681 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    19741 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    19801 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    19861 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    19921 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    19981 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    20041 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    20101 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    20161 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    20221 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    20281 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    20341 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    20401 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    20461 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    20521 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    20581 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    20641 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    20701 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    20761 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta caggactgta
    20821 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    20881 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    20941 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    21001 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    21061 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    21121 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    21181 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    21241 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    21301 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    21361 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    21421 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    21481 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaagtaat
    21541 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    21601 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    21661 aaccgtactt gtatatcttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    21721 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    21781 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    21841 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    21901 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    21961 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    22021 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    22081 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    22141 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    22201 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    22261 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    22321 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    22381 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    22441 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    22501 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    22561 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    22621 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    22681 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    22741 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctccgaaga
    22801 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    22861 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    22921 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    22981 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    23041 tcgaaaagga ttcaacgctt cggcaagata caaaaccaaa taagcgcttt cagctaaatt
    23101 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    23161 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    23221 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    23281 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    23341 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    23401 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    23461 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    23521 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    23581 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    23641 tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga
    23701 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    23761 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    23821 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    23881 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    23941 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    24001 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    24061 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    24121 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    24181 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    24241 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    24301 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    24361 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    24421 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    24481 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    24541 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    24601 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    24661 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    24721 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    24781 aataactaaa tgcaatataa tctaccgtgt tagcttgcaa gtactgttcg tctccatcta
    24841 acattactgg tgcacagtct tcttccttcc atatcctgta aatataagct ggataatgcc
    24901 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    24961 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    25021 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    25081 acatatgatg acaggcttga taaatctctt tttttgttag tgtagcaatg ggttcaagtt
    25141 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    25201 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    25261 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    25321 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    25381 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    25441 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    25501 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    25561 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    25621 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    25681 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    25741 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    25801 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    25861 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    25921 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    25981 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    26041 ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga catattacaa
    26101 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    26161 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    26221 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    26281 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    26341 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt gattgattcc
    26401 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    26461 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    26521 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    26581 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    26641 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    26701 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    26761 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    26821 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    26881 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    26941 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccacca
    27001 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    27061 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    27121 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    27181 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    27241 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    27301 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    27361 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    27421 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    27481 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    27541 tgataaacaa ctgaatggtg ccaccatttt tgttccat
//
LOCUS       DAFEZB010000034        27251 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFEZB010000034
VERSION     DAFEZB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.034"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(161..445)
                     /locus_tag="LP859_001498"
     CDS             complement(161..445)
                     /locus_tag="LP859_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029459.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="LP859_001499"
     CDS             complement(463..1353)
                     /locus_tag="LP859_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL3029460.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="LP859_001500"
     CDS             complement(1373..1591)
                     /locus_tag="LP859_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL3029461.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="LP859_001501"
     CDS             complement(1561..3261)
                     /locus_tag="LP859_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL3029462.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="LP859_001502"
     CDS             3528..4301
                     /locus_tag="LP859_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029463.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="LP859_001503"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="LP859_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL3029464.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="LP859_001504"
     CDS             complement(5125..6096)
                     /locus_tag="LP859_001504"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL3029465.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="LP859_001505"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="LP859_001505"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL3029466.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="LP859_001506"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="LP859_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL3029467.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="LP859_001507"
     CDS             complement(9051..9875)
                     /locus_tag="LP859_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL3029468.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="LP859_001508"
     CDS             complement(9848..10528)
                     /locus_tag="LP859_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL3029469.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="LP859_001509"
     CDS             complement(10551..11489)
                     /locus_tag="LP859_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL3029470.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12597)
                     /locus_tag="LP859_001510"
     CDS             complement(11734..12597)
                     /locus_tag="LP859_001510"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL3029471.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="LP859_001511"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="LP859_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL3029472.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="LP859_001512"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="LP859_001512"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL3029473.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="LP859_001513"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="LP859_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL3029474.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="LP859_001514"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="LP859_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL3029475.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="LP859_001515"
     CDS             complement(19463..20704)
                     /locus_tag="LP859_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL3029476.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="LP859_001516"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="LP859_001516"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL3029477.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22654)
                     /locus_tag="LP859_001517"
     CDS             complement(21731..22654)
                     /locus_tag="LP859_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029478.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23393..24679)
                     /locus_tag="LP859_001518"
     CDS             complement(23393..24679)
                     /locus_tag="LP859_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL3029479.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24672..25940)
                     /locus_tag="LP859_001519"
     CDS             complement(24672..25940)
                     /locus_tag="LP859_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL3029480.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26192..26653
                     /gene="mscL"
                     /locus_tag="LP859_001520"
     CDS             26192..26653
                     /gene="mscL"
                     /locus_tag="LP859_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL3029481.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26712..27158)
                     /locus_tag="LP859_001521"
     CDS             complement(26712..27158)
                     /locus_tag="LP859_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029482.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7743 a         5877 c         4727 g         8904 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
    23401 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
    23461 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
    23521 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
    23581 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
    23641 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
    23701 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
    23761 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
    23821 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
    23881 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
    23941 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
    24001 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
    24061 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
    24121 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
    24181 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
    24241 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
    24301 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
    24361 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
    24421 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
    24481 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
    24541 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
    24601 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
    24661 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
    24721 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
    24781 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
    24841 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
    24901 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
    24961 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
    25021 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
    25081 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
    25141 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
    25201 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
    25261 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
    25321 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
    25381 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
    25441 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
    25501 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
    25561 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
    25621 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
    25681 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
    25741 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
    25801 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
    25861 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
    25921 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
    25981 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
    26041 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
    26101 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
    26161 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
    26221 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
    26281 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    26341 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    26401 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    26461 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    26521 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    26581 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    26641 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    26701 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    26761 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    26821 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    26881 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    26941 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    27001 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    27061 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    27121 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    27181 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    27241 cgtttgcttt t
//
LOCUS       DAFEZB010000035        27184 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFEZB010000035
VERSION     DAFEZB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.035"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(132..1013)
                     /locus_tag="LP859_001522"
     CDS             complement(132..1013)
                     /locus_tag="LP859_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3029483.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            1172..1900
                     /locus_tag="LP859_001523"
     CDS             1172..1900
                     /locus_tag="LP859_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL3029484.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            2056..2373
                     /locus_tag="LP859_001524"
     CDS             2056..2373
                     /locus_tag="LP859_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3029485.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            2366..2974
                     /locus_tag="LP859_001525"
     CDS             2366..2974
                     /locus_tag="LP859_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL3029486.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            3029..3820
                     /locus_tag="LP859_001526"
     CDS             3029..3820
                     /locus_tag="LP859_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL3029487.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            3958..4446
                     /locus_tag="LP859_001527"
     CDS             3958..4446
                     /locus_tag="LP859_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL3029488.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            4497..5369
                     /locus_tag="LP859_001528"
     CDS             4497..5369
                     /locus_tag="LP859_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL3029489.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            5545..5907
                     /locus_tag="LP859_001529"
     CDS             5545..5907
                     /locus_tag="LP859_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3029490.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            5916..6668
                     /locus_tag="LP859_001530"
     CDS             5916..6668
                     /locus_tag="LP859_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029491.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            6682..8484
                     /locus_tag="LP859_001531"
     CDS             6682..8484
                     /locus_tag="LP859_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029492.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            8761..9801
                     /locus_tag="LP859_001532"
     CDS             8761..9801
                     /locus_tag="LP859_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL3029493.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(9853..11997)
                     /locus_tag="LP859_001533"
     CDS             complement(9853..11997)
                     /locus_tag="LP859_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL3029494.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(12354..13727)
                     /locus_tag="LP859_001534"
     CDS             complement(12354..13727)
                     /locus_tag="LP859_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3029495.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(13727..14122)
                     /gene="rbsD"
                     /locus_tag="LP859_001535"
     CDS             complement(13727..14122)
                     /gene="rbsD"
                     /locus_tag="LP859_001535"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL3029496.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(14150..15067)
                     /gene="rbsK"
                     /locus_tag="LP859_001536"
     CDS             complement(14150..15067)
                     /gene="rbsK"
                     /locus_tag="LP859_001536"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL3029497.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(15175..16188)
                     /locus_tag="LP859_001537"
     CDS             complement(15175..16188)
                     /locus_tag="LP859_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL3029498.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(16269..16868)
                     /locus_tag="LP859_001538"
     CDS             complement(16269..16868)
                     /locus_tag="LP859_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3029499.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17054..17755
                     /locus_tag="LP859_001539"
     CDS             17054..17755
                     /locus_tag="LP859_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029500.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17765..20476
                     /locus_tag="LP859_001540"
     CDS             17765..20476
                     /locus_tag="LP859_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029501.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            20927..22555
                     /gene="treC"
                     /locus_tag="LP859_001541"
     CDS             20927..22555
                     /gene="treC"
                     /locus_tag="LP859_001541"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL3029502.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(22615..23796)
                     /locus_tag="LP859_001542"
     CDS             complement(22615..23796)
                     /locus_tag="LP859_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3029503.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            24048..25571
                     /locus_tag="LP859_001543"
     CDS             24048..25571
                     /locus_tag="LP859_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL3029504.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(25640..26368)
                     /locus_tag="LP859_001544"
     CDS             complement(25640..26368)
                     /locus_tag="LP859_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029505.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(26381..>27184)
                     /locus_tag="LP859_001545"
     CDS             complement(26381..>27184)
                     /locus_tag="LP859_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3029506.1"
                     /translation="IIFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKL
                     ADQLLEQQVRMQQDYIKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQ
                     TILPTRQMMNLFPANLSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTK
                     HTVNLVRMLGIILDNAIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQ
                     KGFSTKTNPKNEGLGLFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
BASE COUNT         8717 a         4805 c         5514 g         8148 t
ORIGIN      
        1 cgctgctttc ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat
       61 gcaagaaaga gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga
      121 aaatgtaggg cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa
      181 gaatgctcac cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg
      241 agaatcacag tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc
      301 cccatgtcat ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt
      361 tttggttcaa atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg
      421 gggccaagga gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta
      481 gctaaaggat gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac
      541 tcaatcgaac ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca
      601 ttggcgtcat tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca
      661 ggatggcgtt tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt
      721 atcgttaaga tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc
      781 atagcatggt attgttctac gacatttttt acatatggga gcaataagct tccagcttcg
      841 gtcaattcga tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac
      901 gaaagaatct gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg
      961 aaattcattg tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc
     1021 ctcctactta ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc
     1081 tgtctgttac aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt
     1141 tcgcttcgta gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg
     1201 ttccgttttg tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt
     1261 taaagcacag ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat
     1321 tgcgccgatg aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg
     1381 tttgattgtt catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat
     1441 ttctaaaatc ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt
     1501 agtgacggtc attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc
     1561 tgggatgaca ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc
     1621 agctattttt gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat
     1681 tattttttat attttgttct tattggcaaa atttatttat ccattgacta caccagacat
     1741 gatcttagat tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat
     1801 caacgtgaaa atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact
     1861 gagctggatg tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca
     1921 ttttttgctg actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg
     1981 attttgaaac tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga
     2041 atagaaggtg acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta
     2101 gcagcattaa gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt
     2161 ctctctgtca gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat
     2221 cttgagacct atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca
     2281 atgggaagtc gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta
     2341 gaccggttat tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct
     2401 tcaagaaatt cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt
     2461 agattctgga aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc
     2521 cttaaaatta aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa
     2581 aaatccaaaa agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc
     2641 tgtcctcatt ccactagcac tggggtttat attggttatt gggggactat tggttgcttt
     2701 actagcgctc atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt
     2761 catgatcatc tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat
     2821 cggtgtcggt ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg
     2881 aacaaaaggg cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat
     2941 tacaaaaaga gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa
     3001 acattttttg attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac
     3061 ttatacatta ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat
     3121 ggttactgag acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca
     3181 aacagtttcg gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga
     3241 aaagccagaa ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac
     3301 aggtgcaatc attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga
     3361 tcatgaatta tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt
     3421 atcaatcaaa aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac
     3481 aggaggaaca ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt
     3541 ggataacttg atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag
     3601 caaagaagga aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag
     3661 tggctatact atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac
     3721 aaatactttg ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat
     3781 caatattacg acggatcaag aggacaatga aacagattga tcctgtactc aactggatat
     3841 tttaagaaaa aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca
     3901 taactatagt aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg
     3961 aaggtagaag aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat
     4021 atcactcaac taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac
     4081 ccgaacaatt cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta
     4141 ttgacggtga agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg
     4201 aagtatgatg ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt
     4261 tcagcaggga tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg
     4321 gttcgaacaa aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg
     4381 tacatcactg aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa
     4441 aattaaaaaa gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga
     4501 gagaaaaaag agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag
     4561 ctttagttgg attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt
     4621 ttttaagtat ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca
     4681 gtcctaatca agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa
     4741 atggcttttt ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga
     4801 atttcaatag ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag
     4861 cagtagtagt ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc
     4921 aagattttgt agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt
     4981 atgatacatt caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg
     5041 agttgcaaaa agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac
     5101 gcttgaacca tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag
     5161 caaaagccat tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa
     5221 tggccttaga gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc
     5281 aattgatcaa taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca
     5341 atacaggcga catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt
     5401 taggacggat tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg
     5461 aaagaagaat gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata
     5521 taaagtaaga gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc
     5581 gttgtggtac ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac
     5641 tatgtacaaa ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa
     5701 acagtcaact atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg
     5761 gacttcaagg gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg
     5821 aacaaaaata aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca
     5881 gcactggaaa aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga
     5941 atgtaacaaa agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca
     6001 gcttcgatgt agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt
     6061 cgacactttt gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca
     6121 atcaaaaaga tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga
     6181 ttggctttat tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg
     6241 ctgttcctct ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa
     6301 ttgcagaacg tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg
     6361 gacaaaaaca acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag
     6421 gggatgagcc tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg
     6481 acgaattgaa tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg
     6541 caagttattg ccaacgtatt ttattcatca aggatgtcgg tatccatcaa gaagtgaaac
     6601 gtggagatca aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg
     6661 aacagtaggg ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt
     6721 ttaagagact acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg
     6781 tttgaatcaa tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca
     6841 gttatcattt tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta
     6901 tatgcaaatt cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg
     6961 ggagcaaaag gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc
     7021 gcagctactt tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta
     7081 ttagtggatc agttagatct caatatttcg cattttacac cttttagtat tcacgggttg
     7141 ttgatcactg ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt
     7201 tcgattgtca aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc
     7261 aagcagcata aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc
     7321 ggttactata tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta
     7381 gttacgatcg tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca
     7441 ttactaaaga aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag
     7501 ttaaactttc gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc
     7561 ttggcactgg gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca
     7621 gaaaacaatt ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa
     7681 atcaaagaac tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg
     7741 atttactata atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat
     7801 ggaacaccgc cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact
     7861 gtacaagagg aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta
     7921 gtcagtcaat cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag
     7981 gtaaaagact tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg
     8041 aacaatccat ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg
     8101 tataatggtc tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg
     8161 accatgcttg ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct
     8221 cgttatgaaa tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt
     8281 agagaagtag gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc
     8341 ggattacaaa tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt
     8401 acgatattct tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga
     8461 atcgtattga aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
     8521 gcaaaaatag ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
     8581 tgaacaccgt ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc
     8641 acggtctttt attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag
     8701 aaaacggttt ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac
     8761 atggaaatca atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa
     8821 aatgggacat ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata
     8881 atcgataatg aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa
     8941 aaagaccctt atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca
     9001 ttcaaattag atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta
     9061 catggtggtg cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa
     9121 aacgaagcat cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga
     9181 actttagatg tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc
     9241 caagggatca gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta
     9301 accggtgatc cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt
     9361 gctacgctca ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca
     9421 tttgacttcc aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca
     9481 atgatagtgg gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat
     9541 gctgcggaat tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca
     9601 ggaatcgtga tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa
     9661 ttagtaaatc atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt
     9721 gaaagttttg gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac
     9781 aaaataaata taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct
     9841 ttttaattta ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa
     9901 caaggttagt acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg
     9961 tcttgccgta aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc
    10021 gttgttgacg cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt
    10081 ccatttgatt ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc
    10141 agttgttgct gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat
    10201 cgcgttcaat ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt
    10261 tgcttctttg tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc
    10321 agtcagtcca tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata
    10381 tttacggaat tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt
    10441 gaaggtcacg ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc
    10501 gcttgataat aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga
    10561 tgcgtagatt ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa
    10621 ctgatcagtt gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga
    10681 tgagccgcct gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc
    10741 aaaatcaggc gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc
    10801 atggataaat tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg
    10861 tccccagaag acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata
    10921 ttgctgttcg cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata
    10981 agtcaaacca gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc
    11041 aagatgcttg aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa
    11101 tacagcatca attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat
    11161 accgctttga tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga
    11221 accattgttt ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg
    11281 gtttgtttga ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt
    11341 tgaaccatta ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg
    11401 tgtactaggg acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc
    11461 tttaaattct gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata
    11521 atttacttct ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt
    11581 aatagtgtaa gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat
    11641 atccaaaggt tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat
    11701 caaagaagga gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc
    11761 agccggatca atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg
    11821 gtcttctgta aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt
    11881 tgtgactgtt tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac
    11941 agacggagca atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat
    12001 aaactagtcc ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt
    12061 tggaacagat aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa
    12121 ataaaacaat ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta
    12181 ttgcatagac cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg
    12241 taaattatac caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa
    12301 gaaaaagact gaaatatgtg ataaacatct catacttcag tctttttcat taactattca
    12361 taagacatta ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga
    12421 ctaacagcca gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg
    12481 gtcagactac caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca
    12541 ccagcagctg caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg
    12601 taaacagttg gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc
    12661 gaacgggttt ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca
    12721 aaattggctg ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg
    12781 atcatgacag ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca
    12841 gcgatgattg cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct
    12901 aaaccttcgc caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg
    12961 ataactaatg aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg
    13021 aggaattttt caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac
    13081 gtatactgca tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct
    13141 gaaagtaaag aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat
    13201 ccattcactt taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag
    13261 acggcattcg acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca
    13321 atcgtagtcg ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg
    13381 atcggcgtaa agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct
    13441 ttcggagctg aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga
    13501 tccatgatta tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc
    13561 tgttttaatt tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc
    13621 gcacatggaa ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga
    13681 atctgctcgg acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc
    13741 cactcactaa tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac
    13801 agttattcaa ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta
    13861 acaataattt gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt
    13921 caatcgattg tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa
    13981 gaccgtttga gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat
    14041 ccccgatact taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat
    14101 tgattacttt tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg
    14161 attgttctaa agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa
    14221 cagaagaggc tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa
    14281 gtgtgctgct taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca
    14341 ctttaaaagc agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc
    14401 ctagtgtaat gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca
    14461 aactgttatg atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta
    14521 taatgatatc tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat
    14581 tcaggatcgt tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta
    14641 agctactttc aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta
    14701 ggacttcttg ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc
    14761 ctgctgcgtc taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc
    14821 cagatgtatc gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag
    14881 ccccaacgcc tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt
    14941 tggcaccttt acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct
    15001 tgggcatgtt ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg
    15061 tgttcatttt acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa
    15121 acgttttagt aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt
    15181 caagggtgca gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc
    15241 tttttctggg aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa
    15301 gattggttgt gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc
    15361 atagccaata atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata
    15421 taatccaaaa gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag
    15481 ttctgctgtg atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg
    15541 gtcgtgaggg atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac
    15601 attttccggt ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa
    15661 gtgcatgccc gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc
    15721 ttcagatttt ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt
    15781 gattgcatct gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt
    15841 gagctcggct agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt
    15901 gacgaaattc tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg
    15961 attcgtgata tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct
    16021 agcaaaatag tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt
    16081 tttcggactg aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac
    16141 gccggaaagt ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct
    16201 atctaaatcc tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt
    16261 gccagtactt atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt
    16321 gcagttttcg gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg
    16381 caagcatctt cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca
    16441 tatacaccgt ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga
    16501 agttgaagat caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac
    16561 atattatctg tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca
    16621 gaagcagtca ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct
    16681 acgttcacta aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg
    16741 tttgtcagaa tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt
    16801 tgcatgtcga tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa
    16861 tcattcatga tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc
    16921 gtttatttgt caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa
    16981 aataaataag tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa
    17041 tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg
    17101 agaaacgacc attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt
    17161 gattatactt ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat
    17221 ggatacacct gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga
    17281 taaacagctt actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct
    17341 cgttcctaat ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc
    17401 gcttgatcct gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc
    17461 gtcccaatta tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa
    17521 tcccaaactt cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca
    17581 agtattgaaa ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac
    17641 acataattct gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt
    17701 aaggtctgtt gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga
    17761 ttttatgaaa acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg
    17821 acgattcatt gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa
    17881 aactgctgga cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc
    17941 tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa
    18001 gatcccagat gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa
    18061 cgaagtgatt caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac
    18121 tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga
    18181 agggtataaa atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta
    18241 taccatcacg ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc
    18301 gaacgtcgga aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac
    18361 agatatttat tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag
    18421 caaagcttac aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga
    18481 tcgacccgaa gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc
    18541 acagcaaaaa atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga
    18601 tgctaaaaaa cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg
    18661 aacaatcgct gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca
    18721 attagttgag aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga
    18781 aaaaattgaa aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg
    18841 ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt
    18901 tttctttttc tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga
    18961 aaaccgcagg gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa
    19021 aaaatatgca atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt
    19081 aggaacgaat cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg
    19141 gagtgcgctt attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat
    19201 tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc
    19261 attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc
    19321 gctgtggtca cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa
    19381 gtcaagaatg cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg
    19441 cgtgggattg aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga
    19501 ctatcaagca atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga
    19561 tatgctgcag aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga
    19621 attgagaaaa aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc
    19681 tgatttggat cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa
    19741 aacaggaatc gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca
    19801 tatttcgctt tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa
    19861 aaactatcta ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca
    19921 atcgatgatg gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga
    19981 aatgctttcc gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa
    20041 acaaatcgaa acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt
    20101 cgtattatca ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc
    20161 cgagcgtgta cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac
    20221 gatgtacatt gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg
    20281 tgtaggatat atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt
    20341 tccattagtg atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac
    20401 ggttattgtg atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt
    20461 gaaatcgaat gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga
    20521 aacaaacggc aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag
    20581 ctgattgaac accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa
    20641 tactcaaata aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa
    20701 aagcttttga acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga
    20761 tagacaaata catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat
    20821 aaagataaat tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat
    20881 ataagttgtg gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga
    20941 caaagtcatt taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg
    21001 agatttaaaa ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat
    21061 ttggctaaac cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta
    21121 cacagccatc gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc
    21181 aaagaaatac cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca
    21241 tgaatggttc aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag
    21301 agaagagcca acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga
    21361 tacggggaaa tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa
    21421 ccaagcagta agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa
    21481 aggattccgt ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa
    21541 agaaaatgat ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga
    21601 attgaaccaa gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc
    21661 tacgacgatc gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt
    21721 ctttaatttc catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc
    21781 atttgatttt gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg
    21841 taacggctgg aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt
    21901 tgtagctatc gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt
    21961 gaaccgtggg acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta
    22021 ttcatcgatt tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga
    22081 acaaggattc acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc
    22141 tcgaacaccc atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg
    22201 gttaaaagtt ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga
    22261 ttctattttg tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc
    22321 acaaggagat tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca
    22381 atttgaagga cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc
    22441 catcccagaa ggattcacag aagccaatat tcttatcgat aattattccg atactgttct
    22501 tcatcaaagt ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa
    22561 aaacacaaaa cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt
    22621 aaaataatct tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt
    22681 attgtagcgt tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata
    22741 gataggaata ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac
    22801 gattaagatc gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa
    22861 gtcaccgtgg agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt
    22921 agagtcttgg aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg
    22981 aatgatacat aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag
    23041 taagaaacaa acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag
    23101 aaatgaaccc aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc
    23161 tggtgggaca atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa
    23221 agcgacaatt ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt
    23281 gctgatccgt tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt
    23341 cacgtcgtca atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa
    23401 aaattgtcgg agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt
    23461 ctttgcctgt tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa
    23521 aataatcaca taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt
    23581 gatgactggt tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt
    23641 agcgataatg attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat
    23701 cagcgcaagt aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg
    23761 gatcaaacga ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataaa
    23821 ttcattatag acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc
    23881 gtcaatcatt catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt
    23941 taaccatatg ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc
    24001 gaatcggaac attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga
    24061 taggtatatt ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga
    24121 aagccgagga cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt
    24181 ttacaaaagc aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg
    24241 attcccacga ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct
    24301 ttgtttataa tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag
    24361 aaggcaatgt ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag
    24421 aaggacatga tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac
    24481 atgtatggct agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga
    24541 ttgaagctta cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga
    24601 aacttcaagc actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt
    24661 ttgtcacgca acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat
    24721 cgattgctgg tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac
    24781 actttgttaa agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta
    24841 ttgccagaac gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag
    24901 gattgacgaa tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc
    24961 tcgaagcgtt gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca
    25021 aaaaagaaaa gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc
    25081 tttcagattg ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg
    25141 atcctgtttt tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag
    25201 aatattatac gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga
    25261 tggcatttac gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag
    25321 aaatcttgac ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata
    25381 aggaaagtgg cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact
    25441 ccgaacattt tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg
    25501 agaattggcc aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc
    25561 ttttgcactg ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt
    25621 ccaaactatt cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga
    25681 tggtctcgcc gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt
    25741 gtgtattgat aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt
    25801 ctttgatctt ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg
    25861 gcgctcctag cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat
    25921 cattaaactc tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa
    25981 ttttgctcag gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat
    26041 aatcagtcgg ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca
    26101 catagattat ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat
    26161 ccatttcact gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga
    26221 tcttggctgc attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta
    26281 agttttcaat catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat
    26341 cgtcttcaca aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa
    26401 tcgtttgaga aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct
    26461 ttgtcaattc atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg
    26521 agaacccttt ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac
    26581 gaatcatcac ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag
    26641 cactagctcc ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg
    26701 tatgtttcgt atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt
    26761 cctgtgccat catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat
    26821 ccaacatact tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt
    26881 gatcataata agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa
    26941 aaagcagatt ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat
    27001 ctttgatata atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct
    27061 tattagagat tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga
    27121 aaaaactcgt attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa
    27181 taat
//
LOCUS       DAFEZB010000036        26027 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFEZB010000036
VERSION     DAFEZB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.036"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="LP859_001546"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="LP859_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL3029507.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="LP859_001547"
     CDS             809..1243
                     /locus_tag="LP859_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL3029508.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="LP859_001548"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="LP859_001548"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL3029509.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="LP859_001549"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="LP859_001549"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL3029510.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6193..8424
                     /gene="pflB"
                     /locus_tag="LP859_001550"
     CDS             6193..8424
                     /gene="pflB"
                     /locus_tag="LP859_001550"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL3029511.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8668..9429
                     /gene="pflA"
                     /locus_tag="LP859_001551"
     CDS             8668..9429
                     /gene="pflA"
                     /locus_tag="LP859_001551"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL3029512.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9514..10440
                     /locus_tag="LP859_001552"
     CDS             9514..10440
                     /locus_tag="LP859_001552"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL3029513.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10622..11515
                     /locus_tag="LP859_001553"
     CDS             10622..11515
                     /locus_tag="LP859_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL3029514.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11649..11774
                     /locus_tag="LP859_001554"
     CDS             11649..11774
                     /locus_tag="LP859_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029515.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11908..12540)
                     /locus_tag="LP859_001555"
     CDS             complement(11908..12540)
                     /locus_tag="LP859_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029516.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12688..13230)
                     /locus_tag="LP859_001556"
     CDS             complement(12688..13230)
                     /locus_tag="LP859_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL3029517.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="LP859_001557"
     CDS             13364..13753
                     /locus_tag="LP859_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029518.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="LP859_001558"
     CDS             13757..14521
                     /locus_tag="LP859_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3029519.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="LP859_001559"
     CDS             14650..15597
                     /locus_tag="LP859_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3029520.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="LP859_001560"
     CDS             15727..16467
                     /locus_tag="LP859_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029521.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="LP859_001561"
     CDS             complement(16758..17426)
                     /locus_tag="LP859_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3029522.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="LP859_001562"
     CDS             17560..17847
                     /locus_tag="LP859_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029523.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="LP859_001563"
     CDS             18263..19801
                     /locus_tag="LP859_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL3029524.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="LP859_001564"
     CDS             19798..20763
                     /locus_tag="LP859_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029525.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="LP859_001565"
     CDS             20750..22072
                     /locus_tag="LP859_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3029526.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="LP859_001566"
     CDS             22313..23056
                     /locus_tag="LP859_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029527.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="LP859_001567"
     CDS             23071..24063
                     /locus_tag="LP859_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3029528.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="LP859_001568"
     CDS             24075..24812
                     /locus_tag="LP859_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029529.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="LP859_001569"
     CDS             24802..25524
                     /locus_tag="LP859_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL3029530.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26027)
                     /locus_tag="LP859_001570"
     CDS             complement(25608..>26027)
                     /locus_tag="LP859_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL3029531.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8953 a         4426 c         5429 g         7219 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat aaatccttat
     6001 acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg tcaacgttga
     6061 tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa aacccttgca
     6121 aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac agaaatttag
     6181 gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa agaaggcgta
     6241 gacactagag actttattca aaataactac actgaatatc gcggtgatga cagctttttg
     6301 gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc
     6361 caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac tattacttca
     6421 cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca gacagacaaa
     6481 cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa
     6541 agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg gcgcaaaaca
     6601 cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa
     6661 atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt
     6721 gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca tgatatgact
     6781 ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac tgaacaatat
     6841 cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat ttctaaacca
     6901 gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc agcaatcaaa
     6961 tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga tatctatatc
     7021 caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt gatcgaccac
     7081 ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa ccaattattc
     7141 tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga tggtcgttca
     7201 ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg accttcacca
     7261 gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag aacatatgct
     7321 agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca gcttcgtgaa
     7381 caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa
     7441 gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata tgccatcaat
     7501 ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc aatcacatca
     7561 gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga ttggttagca
     7621 gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata ttcttatgaa
     7681 gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac aggtattgct
     7741 gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc
     7801 cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc taaatacgga
     7861 aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt catgggacaa
     7921 atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt gacgattact
     7981 tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga agctggaaaa
     8041 ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt attagctagc
     8101 ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc aaatacccaa
     8161 acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat
     8221 gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt attcagtaat
     8281 gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac gatccgggta
     8341 tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga tgtcttgatg
     8401 agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga
     8461 aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa
     8521 cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca taacgaagcg
     8581 gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg tacttaaaat
     8641 gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta tgttcattct
     8701 atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt tatgcaagga
     8761 tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg cggagggaaa
     8821 gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta ctggggagat
     8881 aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt tttgatcgaa
     8941 ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg cggaaaacct
     9001 tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta tacagattta
     9061 ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac gcattgggac
     9121 aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt
     9181 cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg actagacaac
     9241 tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca tacgatggga
     9301 aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga accacctaca
     9361 aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac aaaatattta
     9421 acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt tttgctatta
     9481 ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt ttttggtcac
     9541 caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt gcaaaatgaa
     9601 ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac acaatacgca
     9661 ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc agaaaatgca
     9721 gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat cagcgaagta
     9781 aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc taatccattg
     9841 tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact atataaagaa
     9901 aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat catttcagat
     9961 acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc aaaagaactt
    10021 gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa agcaggaaca
    10081 aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag tttcccaatg
    10141 ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga agtgatggat
    10201 cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg ctacgatctg
    10261 ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt tgctggtaaa
    10321 ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg tgctttgctt
    10381 aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt atttagctaa
    10441 tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata aaaagaaaca
    10501 tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg actcttgttc
    10561 ttcctccttt ttacgtaaaa gccttttaag taaaataaaa cggatagaaa agagaacata
    10621 tatgaactat tattcaaaaa ataaggaaaa cttttatcaa aaactgaccg atgatccgct
    10681 tttttcttca ttgaccgatt atctatatga acatcgagaa caagaaacga ttctaagaga
    10741 attgaaaaaa gaattttcgc aaaataaatt ttcccatttt cttgatttat tgatcgatgc
    10801 aggattaatc aagagggaag aacgaagata tcatttgaac tttccaatat ttgatcctaa
    10861 agattacttg caacaagcca cttcggctgc tgaaaccata gctgaacaat tgaagagact
    10921 ctctgttgat gaacaaaaat tagcgatggg agaagttatt tgggcgtatt gtttcgaaga
    10981 tgaacggaaa gaagcgtatt tttatggagt aagaaacagc cgagagacag aattgctgag
    11041 gacaactgca ggaaatgaga agtaccgttt tatcacactt tcctctatag aacacttccc
    11101 gcttactttg gctaattact tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt
    11161 caaagaatta gccgaactta ttggagacgt aaatgaatcg tacttttttg atcaaataga
    11221 agttatcgtt gacaggatac gtaagaataa atataaaaat cgccggccta gtattttcca
    11281 tcagtctcta cttgtgacga atactataaa ggaagaagaa tcattcacat tagaattacc
    11341 tattgtcgaa aaaaacaatt ttgagattga attaccaaca ttggatcctt cattgacaat
    11401 ggaggaaatg gcctttctta aacgacaaat cttttctgag ctgagcaaga aatttatacc
    11461 gcatgcattt tcttatatca aagaatatgg aacagtttta gtgagtaaaa cctaagagag
    11521 cagtaagcaa aaattatact gactcttgca ggaggagcct ttctcttcct ttatggttaa
    11581 aagaattatt tgagtatttt gcctatctac cacctattag attcactaca tggtctggaa
    11641 acaaaggaat gcaaaaaaat ggtgagaaat gtggaatgac aaaggaagta gtgataagaa
    11701 aagttagatt tttaaataat caatattatg attcagtcaa gtatggcatt ttatgggaag
    11761 aactagctga ttagattagg aagtccattt gataatccag aatcactaag aaacgaatgg
    11821 cgagtcatta ataatttatc caatcttggg ttattctttt agattgaagt gtttaaaact
    11881 aaaaaagcat ttatgaagtc gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt
    11941 ttttctttgc cacataaata cacctagact gacgatgaag agcatatcca acagatttcc
    12001 tgcgacttgg atcgtcgaag ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa
    12061 caagctgtaa ataaaacagg tgacaggaac taatcgaaag aagagcggac atttcaattg
    12121 gctaaaaatg agtgaaaaaa taaaataagt aaaaatggat aaaatcacga ctaaaccatt
    12181 catgataaag aagaaaagcg tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga
    12241 aaacgtttta aatgcaatca atacgcttga agcttcgttt aacgtaccag gatgtcgatg
    12301 aaaaaaatag gcaccgaaat aaacagaaaa cagtaagccg caaagagaag acaaaatcaa
    12361 aaaaaacgga ttttcttcgg cttctgtgat atcagaatga tccaaaagca accagcctaa
    12421 tgcactagaa ggcgcatgtg aggtcttaag gtatgaataa catagaaaaa ataagatcgt
    12481 caaagttgca aaaaaagagc ctaaatgtaa aaaattagtg gttctacgtt gaatttccat
    12541 aatatctcca aattaaattg ataagtatat aaagtataat aacaaattga tgagtatatg
    12601 tcgatgtttt ttaatcaaaa taataaataa aaactgtcta acatttaaaa tttaatttag
    12661 atattagaca gtttttaagt ttgtgagtta ttttccttgt cgttttctca cttttttagt
    12721 ccagaaacca cctgaaatat attttggctc ataagaaatg ataaaagcac gctcttcgat
    12781 ttctttgatc aggcgataca gttcacgttc gctttttcta ggagaaagta tttcgagaac
    12841 tagccgctca ccttccagcc ccattgcaga agtctgtgtc acaccataac cgtgttctct
    12901 cagtataccc ggcaaacttt tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat
    12961 gatgtatcct aatgccaatt tgtcttcaat caaaattccg acactgatac ctactgcgta
    13021 acctaacgca tagacaaata aattcagtgg attatctaaa cgatctaaaa caagggataa
    13081 gccgacgata taaatcgtga tctcgaccat actgacaagt ggagcaatca gtcgatagcc
    13141 tttcattgtc aacatgaaac gtaacgtgtt cagagtgata taagcaaaat taatgaggaa
    13201 gatcatcccc aacatttttg gatcaatcat ttgtaaaact cctttcattt atataacaac
    13261 aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt tatacataca aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAFEZB010000037        25502 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFEZB010000037
VERSION     DAFEZB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.037"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            133..3660
                     /locus_tag="LP859_001571"
     CDS             133..3660
                     /locus_tag="LP859_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL3029532.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LP859_001572"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LP859_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL3029533.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LP859_001573"
     CDS             7448..7789
                     /locus_tag="LP859_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3029534.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LP859_001574"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LP859_001574"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL3029535.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LP859_001575"
     CDS             9146..11566
                     /locus_tag="LP859_001575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL3029536.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LP859_001576"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LP859_001576"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL3029537.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="LP859_001577"
     CDS             13000..13737
                     /locus_tag="LP859_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3029538.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="LP859_001578"
     CDS             13751..14566
                     /locus_tag="LP859_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL3029539.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="LP859_001579"
     CDS             14579..15226
                     /locus_tag="LP859_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3029540.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDRFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="LP859_001580"
     CDS             15242..15898
                     /locus_tag="LP859_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3029541.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="LP859_001581"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="LP859_001581"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL3029542.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="LP859_001582"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="LP859_001582"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL3029543.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="LP859_001583"
     CDS             18240..18758
                     /locus_tag="LP859_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL3029544.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="LP859_001584"
     CDS             18865..19365
                     /locus_tag="LP859_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3029545.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="LP859_001585"
     CDS             complement(19645..20565)
                     /locus_tag="LP859_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL3029546.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="LP859_001586"
     CDS             20730..21743
                     /locus_tag="LP859_001586"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL3029547.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="LP859_001587"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="LP859_001587"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL3029548.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="LP859_001588"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="LP859_001588"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL3029549.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="LP859_001589"
     CDS             complement(23383..24144)
                     /locus_tag="LP859_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3029550.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="LP859_001590"
     CDS             24333..25427
                     /locus_tag="LP859_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL3029551.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8449 a         4424 c         5383 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagataga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DAFEZB010000038        25461 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFEZB010000038
VERSION     DAFEZB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.038"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(20..1039)
                     /locus_tag="LP859_001591"
     CDS             complement(20..1039)
                     /locus_tag="LP859_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3029552.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1061..2068)
                     /locus_tag="LP859_001592"
     CDS             complement(1061..2068)
                     /locus_tag="LP859_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL3029553.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2061..2726)
                     /gene="pgmB"
                     /locus_tag="LP859_001593"
     CDS             complement(2061..2726)
                     /gene="pgmB"
                     /locus_tag="LP859_001593"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL3029554.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2719..5013)
                     /locus_tag="LP859_001594"
     CDS             complement(2719..5013)
                     /locus_tag="LP859_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL3029555.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5273..7438
                     /locus_tag="LP859_001595"
     CDS             5273..7438
                     /locus_tag="LP859_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3029556.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7742..8575
                     /locus_tag="LP859_001596"
     CDS             7742..8575
                     /locus_tag="LP859_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL3029557.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8677..10377)
                     /locus_tag="LP859_001597"
     CDS             complement(8677..10377)
                     /locus_tag="LP859_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029558.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10884..12044
                     /locus_tag="LP859_001598"
     CDS             10884..12044
                     /locus_tag="LP859_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3029559.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12060..12503
                     /locus_tag="LP859_001599"
     CDS             12060..12503
                     /locus_tag="LP859_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029560.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(12986..13516)
                     /locus_tag="LP859_001600"
     CDS             complement(12986..13516)
                     /locus_tag="LP859_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL3029561.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13621..14172
                     /locus_tag="LP859_001601"
     CDS             13621..14172
                     /locus_tag="LP859_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3029562.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14325..14798)
                     /locus_tag="LP859_001602"
     CDS             complement(14325..14798)
                     /locus_tag="LP859_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3029563.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            14936..16408
                     /locus_tag="LP859_001603"
     CDS             14936..16408
                     /locus_tag="LP859_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL3029564.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16405..17115
                     /locus_tag="LP859_001604"
     CDS             16405..17115
                     /locus_tag="LP859_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL3029565.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17467..17751
                     /locus_tag="LP859_001605"
     CDS             17467..17751
                     /locus_tag="LP859_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL3029566.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18032..18451)
                     /locus_tag="LP859_001606"
     CDS             complement(18032..18451)
                     /locus_tag="LP859_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL3029567.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18453..19037)
                     /locus_tag="LP859_001607"
     CDS             complement(18453..19037)
                     /locus_tag="LP859_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL3029568.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19084..20559)
                     /locus_tag="LP859_001608"
     CDS             complement(19084..20559)
                     /locus_tag="LP859_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL3029569.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20740..21051
                     /locus_tag="LP859_001609"
     CDS             20740..21051
                     /locus_tag="LP859_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029570.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21166..23502)
                     /locus_tag="LP859_001610"
     CDS             complement(21166..23502)
                     /locus_tag="LP859_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3029571.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23644..24312
                     /locus_tag="LP859_001611"
     CDS             23644..24312
                     /locus_tag="LP859_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029572.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24315..25358
                     /locus_tag="LP859_001612"
     CDS             24315..25358
                     /locus_tag="LP859_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3029573.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
BASE COUNT         7820 a         4628 c         5198 g         7815 t
ORIGIN      
        1 tatttagtga gtcagctatt tagtatgggt tttctaaagg aacgactgtc tctcttttga
       61 tcaagcgatg agggatgacc aaacgtacgc cactgctcaa ttcttcatgg atcaattcgc
      121 gcagcttttg catagcgaca cgtcctaatt cggagatatc gatatcgata cttgtaaggt
      181 aagggtgtgt taatgttgag aagatcgagt tgttgaagct aatgacggag agtgtctctg
      241 gaatgacata gccatacatt tgcgccaact gcatcgtgcg aagggcgaag atatcgtcaa
      301 tgaccaccaa tgctgtggct tcgttttctt tcaacgtttt ctcaaaggac agataatcct
      361 ccggatgctc tagtactaca ctggataacg caggaagacc agccatcatc attgcttttt
      421 gataaccgaa atatcgttcg aaatacaaac tttcatgagt cgtgtttgtc acaaacagga
      481 tattcttatg tccattctgg atcaaaaatt ctgttgcttg tttacctaga agctggttat
      541 cattatctac ataaacgatt tcgttctcat gatgataagg ctgtccgatc aaagtgaaag
      601 ggatgtggtt ttggtataaa taatcaatga cagtatcttg actatctgaa tacactagaa
      661 taaaaccatc tacttgtttt tgtaaatgca tacgctggac gttctctaac agcaattcaa
      721 aatttttagc agtagcgatt gctgtagtca tatcataatt ccgcgcttct tcgttgattg
      781 cttccatgat ctcaagataa aatgggttac cgatccgctc ttttgaatca agagggggta
      841 agattacgcc gatggcactg gaaatgcgtt ttcctagatt gcgggcagtc atgttaggaa
      901 cataacctag ttcatccata atttttcgga ctttcttctt tgttgtctcg gaaatactag
      961 gatgatcgtt gatgacacga gagacggtcg aggtggcaac accggctttt ttcgccacat
     1021 cttttacggt aatagccatg ataaaaacac ctttctcttg ttattgtgca gagaaccgat
     1081 aaatcgtctg actgatccgt tcttgaccag gatggaggtt gatcgatcca aatttgggga
     1141 aatgaacgat atcagggatt tcttgcggtt cgattgctaa tccgtagttt gaatgcatct
     1201 gttttccatt gacagagaaa tcagcttcga atccagtcgt agaaaataat accatacttt
     1261 tattagaagt tgctatagtc atttggcgac cagaatgctt ctcgaataat tgtaaggaag
     1321 ttttggaaag cctaggatga tgcaaaagaa atacatcatc tagtccatct ggataacatg
     1381 caaggatctc ttgcagtgtt ggggattttc taaagtcgat tggcagtttt tctttttttg
     1441 tccaatttcc agtaggcagt ttttcttgat ctaattcaag cacaccatct aaaaagaccg
     1501 ttaactgatg ttcatagatg tctcttgttc catttcctga aaggttgaag taggcatggt
     1561 tggtcggatt gcagatggtt tcaacaaggc tactagcaga ggttgtcatc tctaattcgt
     1621 tttttgttaa acggtaagtg atcgtagcag tgattggtcc tggataggta gaaaattcat
     1681 cttttaagta aaagaccaca cctattgatg atggagtttt gaaaacttct acttcccaaa
     1741 attggaaaga ccagcctttc gtgccaccgt ggatatggtg tgatccgcag tttttctcca
     1801 atctatgctc accccaagaa gcatcccgga tccttccggc tacaggtccg acagttgctc
     1861 caaaaaagct tttgtctgtt agaacatctt cgaaacgatc gtaagacaat aaaacatttt
     1921 ctgattttcc ttctttgtct ggtgcgaaga tttggtgcag gcgggcaccg taattgagaa
     1981 gaacgacttc taagtagtcg ttcttcaatg tgattttctt tatttcagtc tctggaactg
     2041 gtacgacgga gatctctgga ttatccatgg tcatgccact tttctttcaa gtaatcgatc
     2101 gtaagaaaag tcgtatcagg gacaagcggg atctcctctc caaggtcttc agcacgtccc
     2161 acgccaattg gttgagcgcc acttgagagg atggcttgga taccggcttg cgcgtcttcg
     2221 atcccgatac attcttctgc ttttagtccg acagcttttg ctgcaaggag gaagatatct
     2281 ggcgcaggtt ttccgtttgt tgcttctgcc gggtcggcaa tagcatcaaa gtaaggtgta
     2341 agacccattt tttcaagtag gaatggcccg tttttacttg cagaagcaag agcaatcttg
     2401 attttttctt cttttaaaga atccagtaaa ggcacgaccc cggggaagac atcttttggt
     2461 tcgatcgtct ggatcatctc taaataataa tcgttttttc ttttcgctaa ttctgcaaat
     2521 tcttcctttg aaaaagagtg ttcttttccg ccataagcta aaagcaaaga gagtgagtct
     2581 tctcggctga cgccttttag ctgttcgttg aatgtgtggt cgatcgtaat gccgatttcc
     2641 tgaccaagct ttcgccaagc acgataatgg aattcagcag tatcggtaat gacaccgtct
     2701 aaatcaaaca ataccccttt aaacatttgc ttcctccttt cttagcgtta tttctagacg
     2761 atccgctaaa gaataagact gttcatagac gtaaatttct agaggagaac cttctaataa
     2821 ctcgaaagta acttgttcat ctgtgactga gatgaacaat aaacgtccgc gataattgat
     2881 atggaaagaa taagctttcc aagtttttgg cagaaatggt gcgaatctca actgatcatg
     2941 ggcagttttc atttgagcaa atccttggac aatcgacaac cagctaccag tcatggaagt
     3001 gatgtgcaag ccgtcttctg tgtcgttatt gtaattatcc aaatccaagc gagctgttcg
     3061 ctggtacatt tctacagctt tgctttccat tcctaattct gctgctaaaa tcgaatggat
     3121 acttggcgaa agggaagact catggacagt cattggttca tagaaatcaa agtttttttg
     3181 cttttcttct tttgtaaaag tatcattgaa gaaataaatc ccctgtagta catctgcctg
     3241 cttgatgaag caagatctaa ggattttgtc ccaagaccaa tgctgattca gaggaagatc
     3301 gcttgtggga atatcggata caggcatgag atctttatct aagaatgtgt catgctggac
     3361 aaagattcct aattcttcgt caactgggaa gtacatgttc tctgcgatat ctttccattt
     3421 ggctagttct tcttcgctga tagtgatagc agcttctttt tgataagtta aataattttc
     3481 tcgcgtgtaa cgcaagaccc aagctgcgat cgtatttgta taccaattgt tattgatatt
     3541 gttctcatat tcgtttgggc cggtcacacc gtggatcatg tatttatttg ctcgttttga
     3601 aaaatggaca cggtctgccc agaagcgtgc gatttctgct aaaacttcca gtccttcatt
     3661 tttcaaatat tccgtatcgc cggtgtaatt cgtataattg tagattgcat aggcaatcgc
     3721 gccatttcgg tgaatctcct cgaatgtgat ttcccactcg ttatggcatt cgacaccagt
     3781 gaaggtgacc ataggataaa gcgcgccttt taatccttgt tgctgagcgt tgtggattgc
     3841 ttgcggcagt tgattatgcc gatatttcag cagattctta gttacttcag gtttggctaa
     3901 agcaagataa agagggacag catacgcttc tgtatcccag taagtggctc cgccgtattt
     3961 ttctcctgtg aatcctttgg ggccgatatt caatcgttca tcttcgccgt agtaagtaga
     4021 gaatagttgg aacaaattga atcgaatacc ttgttgtgct tcttcgtctc cttcgatgac
     4081 cacatcggca agcttccatc gttctgccca agccgctgtt tgctcttctt ttgcttggat
     4141 ataattgttt gtctggttcg ttaattctga gacagctttt tctgcttgct gcgcttctgg
     4201 aatatctcta ctagtaagaa cgataatttc tttttccact gaccatgtct caccttgaga
     4261 taattctgca gacaagcaac catgtaatgc taaagtatct tcgtgatagt cagggatgac
     4321 tgattttcca ttaacgaaat ggcgcattgc tgcagtaaca gtaaattgtt caatcccaaa
     4381 gttgtttgga atagttttag tcgtcaaata gccgatttct tctatagttc cacgatcaat
     4441 ctcattccaa aaatgttcat catagttgct gtcttcgttt tgaacatttc catccaattt
     4501 tgatacgagt ttgactgttc cgctaccttc aagcatttct atagttaaat agatataagc
     4561 tgattctttt ttgacaatgc tcagaaaacg ttcgaaggaa atgcgtactt tattagtaga
     4621 agtagtgatt gtgaatgtac gagataatac accatttttc atatctaatt cccagtaaaa
     4681 gtcttctggt tccaaagaag ccaagtcgat cagctgatcg ttgatcgaaa tatctatcgc
     4741 gataaagttc aaggcgttga tcacttttcc aaagtattct ggataaccat ttttccacca
     4801 gccgacacgt gtcttatcgg gataccagac acccgccaaa taagtacctt gatggtggtc
     4861 ccctgagtaa gtttcttcaa agttcccgcg catccccata taaccgtttc caatacttgt
     4921 taaagattct tgcagtcgaa gatttttttt atctaattgt gtcgtgctga ttttccatgg
     4981 atcaatagca aatagtcgtt tgatctgttt catttttctc ctcctaatga gctgttttat
     5041 acgttcataa taaacgcaac cgattgcaca agtcaataga caaaagaaaa tatttcatag
     5101 aaaaaagctt aaaaaaataa atttttagga gaaaattata gaagggaaca cacgtattat
     5161 aggttttata gaatggtata gacagaaaaa taaaaaataa agcatttaca aaaaagaaaa
     5221 cgcacgctat aatacgttta acgaaaccga ttgcgtaaag gagctaaacg gaatgaaaaa
     5281 gatatttagt tttgagtttt ggcaaaaatt tggaaaggct ttaatggttg tggtagcagt
     5341 tatgccggca gctgggttga tgatcagtat agggaaatca attcccttga tcaatcctga
     5401 ttggacgccg cttgtcacaa cgggcggagt aattgaaaat attggttggg caattattgg
     5461 taatcttcat attttatttg ctttagcaat aggaggaagt tgggcaaaag aaagagcagg
     5521 cggagcattt gcagctggga tctcatttat tttaatcaat cggatcactg gggctatctt
     5581 tggtgtcaca agtgacatgc ttgcgaatga agatgcattt acccacactc tgttcggtac
     5641 gaagatcatg gttaaaggat tttttacaag tgtattggaa gcaccagctt tgaatatggg
     5701 agtattcgta gggatcatcg ctggatttgt cggggcaatg gcttataata agtactataa
     5761 ttatagaaaa ttgccggatg ctttgtcatt tttcaatgga aagcgctttg ttccgtttgt
     5821 tgttatctta tggtcaacaa tcgtggcatt gattcttgct gtggtgtggc caaacgtaca
     5881 ggctggaatc aataattttg gtttatggat tgcagaatca caagaaagcg caccgattct
     5941 agcaccgttt ttatttggaa cattagaacg tttactattg ccatttggat tgcatcatat
     6001 gctgacgatt cctatcaact atacacagct tggcggatcg tatgagattt tatcaggcgc
     6061 acaggcgggg acacaagtat ttgggcaaga tccgttatgg ttggcttggg ccactgattt
     6121 agtgaatctt aaaggtgcag gggatatgtc gcaatatgag tttgtgttgc ataactggac
     6181 gccggctcgt ttcaaagtag gacaaatgat tggttcttca ggtatcttga tgggaatggc
     6241 ttttgcgatg tatcgtaatg tcgatcctga taagaaagca agatataaat caatgtattt
     6301 ttcagcagcg ttggctgtct ttctaacagg tgtaacagaa ccattggagt tcatgtttat
     6361 gtttgctgct gtgccattat atgtcattta ttcagtgatc caaggggctg catttgcgat
     6421 ggcagatatc ttgccattgc gggtccattc atttgggaat atcgaattgc tgactcggac
     6481 accgttagct atcaaagcgg gtctaggtgg agatcttatc aacttcgtgt tgtgtgtgat
     6541 cgcttttggt gttgttacgt atttcttagc aaatttcttg atcaaaaaat tcaactttgc
     6601 tacaccggga cgtaacggaa attacgacaa tgacagtgaa gaaaccgttg tatctaacag
     6661 cggaacagga acagcagatc agcaagtggt ccagatcatt catttgctgg gaggaaaaga
     6721 aaatatcaaa gatgtcgatg cttgtatgac tcggttacgc gtaagtgtaa atgatcgaga
     6781 aaaagtaggg actgaagaag cctggaaacg agcaggggcg atgggattga tcgtcaaaga
     6841 caatggagtc caagcagttt atggaccaaa agcagatgtc ttgaaatcag atatcgaaga
     6901 tctgctgcaa tcaggagcag agattccaga gccagaaatg gtttctacag gaaatggaca
     6961 agcaggtagt aagcaatact taggtataga acaagaacta gtttccgcag cttctggaga
     7021 agtgattcct ttatctgaag taaaagatcc ggtattctca caaaaaatga tgggtgacgg
     7081 ctttgctgtt attccaacat caagagaagt tgttgcgccg atcgctggaa aagtgacaag
     7141 tattttccct agcaaacatg caattgggct ggaaacgaaa gatggtatcg aagttttgat
     7201 ccatatggga atcgatacag tccaaatgaa tcagccagct tttgagattg ctgtcaaaga
     7261 aggccaagaa gtaggaacag gaacgaaatt agctgagatg gatcttgatg tgatccaaaa
     7321 cgaagaaaaa gatccaacga tcatgatcgt atttacaaac gataaagtag aagaagtagt
     7381 gatcaagcaa ttaggtacag tgacagctgg taaagtgatc ggctccatta aaatgtaagg
     7441 agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa cagccaaaca aaaatagctc
     7501 ctcctatttt tcgtttggtt gggcttatta ccgagaagca gcagtttgaa tactgtttag
     7561 tggagaggtc gtgaaaaagc tgccctgcac caagtgataa aaacagccaa acaaaaaata
     7621 gctcctggtc cattgagctt attactgagg agttagcagg agaacactat ttgctggaga
     7681 ggttgtgaca aaaattttgt cacaaccctt ttgtttcgaa atgaagacaa ggagaaaacc
     7741 aatgaaattc atgacactca acacgcatag ctggcttgag ccagaaccag agaaaaaatt
     7801 gcaagaatta gcagataaga tcctgttaga agattatgaa gtgattgctt tacaagaaat
     7861 caaccaatta ctggaatctg aagaagtaga acctgcgaag ctaatgaagt tctgtccggt
     7921 aaagaatcaa gtacctatcc gtaaagataa ttttgcctac cgtttagttc agcttttaaa
     7981 ggaacacgga aaagagtatt tttggagttg ggaaatgagc cacatcggtt atgataaata
     8041 tgaagaagga aatgcactgc ttacgaaaaa aacattggaa agtcaggtcc ttaccgtctc
     8101 ccaatttcaa aagaaagaag attatcagac acgcaagatc ttgattggga aaacaaagat
     8161 agatgatcag gacatttttg tctcgagctg ccacttttct tggtggacag ataaaaaaag
     8221 tggcttttat tttgagtgga aaaacttgga aaactacttt ttggaaactc gcgtaccact
     8281 ttttttccta ggtgatttca ataatccagt agactctcaa ggttattata ctgtaagaga
     8341 aagttgcctt cttcttcaag attcgtatgt tgtcgcgaat gaaaaaggaa aagcagctac
     8401 tgtggaaaag aaaatagatg gttgggaaca aaatacagaa aaattgcgta tagattttat
     8461 tttcgtccct gaaggaatgc aggttaaaaa atatcaacgg atttttgatg ggatcgatag
     8521 tccaatcatc agtgatcact acggggtgga aatcgaatta gatgtgaacg aataaaaaat
     8581 ataacatata acataaaaaa actgattaaa ataaaactct ccaaacgatt ataccgtttg
     8641 gagagttttt tgatcggatg tatttatccc ttttgcctaa gcacgtaaaa gcttgtatct
     8701 atgataaata aataaacata gcaaggtaaa ccagccaatc aaagtaagag aaagaatgaa
     8761 atagaaatag attggcgttt gataatgaag cgtgagctcg ttgattcctt tttgctgctt
     8821 cacgattggg gtcccgatat ctgttacttc ataatcagtc aatgtttttc cattttgctg
     8881 tagcacagtc cgatcgtaga cgataacagg gatatgaaca gttttgtccc cagtgttttt
     8941 ccacttgatt gtcaaaagac cgcctggatg tgtttttata aaagttgttt cgttttctag
     9001 tatttcttcg ttataccggt cgtatttatt gtcttttgtc tccttataga ttggcagata
     9061 gtcaggtgtg gttttttgga aaacgaacaa gctggtagac atatctttgc tgaagaatgt
     9121 tttccttgct tcttccggcg tatcgaatag ataagtatgt cgactgacaa atgtttcatt
     9181 ttcccacttt tccagatgct ggtataagtt ctggatcgtc tgtgctgaaa aaataagaat
     9241 gccaaatata cttaaaagac ggaaccactt ttgttcagac cactggttca gtgaaagggc
     9301 aaggtaaagt agaagcaata gcgtaaatgg cacgaaaaat cgaaatggaa actggatcaa
     9361 actaataaaa ggcacatttt tttcgatcaa tgtcgtccat ggaatcaagc tagtagaaag
     9421 aaaaaagaaa acaagacttg tgtacaacac gattttttgg aataaggtgt tcttttgttg
     9481 aaagaaaata aaaagcaaaa aacctgataa tatcaatata ggaaaggcct tagggtaatg
     9541 gagccaataa ctgccatcca gatctatcgc cttttgactc atttctctgt taacaaaggg
     9601 aggaagaatc ttattcccac tgtaaacaat agctaatcct gcccaaatat ttgctgtcag
     9661 aacaaaataa agcaagaccg acttaatcag ttgaagcagt gcttttcctt tttcagtctg
     9721 gttaaaaaag taataaagat aaaaagggat atacatcaaa gctaacatga tcgaagacag
     9781 taaatggatc tgtgtcataa gtcccactga gacagcgact ttgataacag ggaacttttt
     9841 atgaaggacg aaatcagcaa tagggatcat acataaagga taaaaacaag tcccccagct
     9901 agtgaatcct tggttgatag accagtattg aatagaatac gtactcatga ataaaacggc
     9961 tactggtaca cttacccaat ttcgaacatt gacatatctc agtaaaatca gcatagaaaa
    10021 tcctgaaata agataaagaa tgaaatttga aagaatctga taatgaaacc agctacctgc
    10081 aagcaacacg atcaatcctt gtaaataagc gaaaaatggg ccgtaaacgg cattcacgat
    10141 cctgccactt tgctggaatc cataaaggga aggaaaataa tggaaatccc catatttaat
    10201 ttgttgcgcg gcatcataga accgattgta atggaatggg gcatcagcac ccaaaattag
    10261 tttatgtgtg aataaatgag gcatgagaaa tgccaaagca atgaaaaaaa ttgctaaaaa
    10321 tgggccatat tttttcaatt gtcgaactcc tttgcaaact ttagataaat tcatcatata
    10381 tcctatgcga gaggtaggca agaaattaga aaattcatga aaatatttat gaattttaca
    10441 agaaaaacat aagtgaaagt tctaaggaga gaggatgcga cataagtttt gttgcatctc
    10501 cttgttttga aacagaattt ttcttaatac tcgaaacaaa gtagcttttt catgacctct
    10561 cggtaaacgg tgttcaatca gctgcttctc ggtaataagc tcactagcct agaaaatatg
    10621 agaagctatt ttctgactag cgctcttatt actcaaagca taacgcagtt ttcacaacct
    10681 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc caacaaaaaa
    10741 tatgagtagc tatttttttg ggctgtactt attacttgat gcaggacagc tttttcacaa
    10801 cctcatcggc tttcattttc tatttttttc ctgaacccat ataataaaag aaagaacaaa
    10861 aaaacttgca agggaggaca catgtgaagc agagtaagca aaaacagtct cgtcgacttt
    10921 tgtttcgttt gtttttatta atatgtatcg tcgtagggtt actgatcgct ttatatccat
    10981 tttatgtgga ttctttaaat agttttattg atcaaaaacg tatcgaacaa gtacaaaaac
    11041 ggaccgcagc tgaaattgaa gtgcagcgaa agaaaatgga ggagaaaaac caacgattaa
    11101 ctgaccaagg attcaatcct ggtactgatc ctttcaatga aaagaacaga aatgaaagta
    11161 caacatccag ccaattagag gaatggctga tcggaagtat caatatacct aagatccaat
    11221 taaatatttc actttatgat cggctcaatg gtaggatcct tgaaaatgga gcaggcgtct
    11281 tgcaaggaac ctctttcccg cttggtggaa attcgacgca ttcagtgatc tctgctcaca
    11341 gcggtttacc taatcgacgg ttatttaccg aattagatcg tttagaaaac ggagacacgt
    11401 ttattttgac tgttttgggg gagaaattgg cttatcaagt taaaaatata caagtggtac
    11461 tgccagatga cacgtctgtt ctaacgattt cagaaggaga agacttagtt accttgctta
    11521 catgtacgcc gtatatgatc aacacgcatc gtttgctagt gacaggacat cggattcctt
    11581 ataacgaatc ggtaaaaaaa gaagaaggaa aaggaaatca ggaaagaacc atgcggcaat
    11641 ggtttattct tgcaggaacg ataatagctg tagtcatact gttagtgttt atcggacgac
    11701 taatctatca gtatagatta agtaaaaaag tgcttgattt ttcatttatt attagtgatt
    11761 cgtcagggaa accggtaaat ggtagttcat tcattttgaa acataagaaa aaaacactta
    11821 ctagaaatgg tgtgccgttt tcagtacaaa gcgaccatca tgggaaagtc aagctggatc
    11881 aattgcctgg tggaacttat cgaatcgttt cagctgatga tcctaaagtt gctgtttctt
    11941 ttggtattag aaaagtaaaa caagagaaga tatacttttt tgaggggcga aaacttgtaa
    12001 aagagcttca aaaaaatggt ttttatttta aaatcaatga ataagtaagg agagaaaaaa
    12061 tgatccgcac aacaacaaaa ttgtctgcca aagaactgga atctgttcta tcgatttggc
    12121 tgcatgctaa cttggattct catgaattca ttccatcaac ttattgggag aatcaagtag
    12181 aatctgttag gaaagcttta ccagatgcag tgatttactt agcagaaata gatcaagaaa
    12241 ttattggttt tgctggggtg attgatcagt ttatcgcagg aatctttgtg aaaagggaat
    12301 accggcaaca aagaatcggt acgcaactaa tgaaaaaaat aaaagaaacc aataaaacac
    12361 tgactttgac agtctatcaa aaaaatcaat cagctattca tttctatctt tcacaaggtt
    12421 ttacaattca aacacagcaa aaagatgaag cagtaaatga ggaggaactt gtgatgaaat
    12481 ggaatcaaaa cgctgatttt tgatgaacag aataacgaaa taatgctccc agcctaattt
    12541 tttgctggga gcattatttc attttgaagt cactaattta actgtgtgaa tatgcagttg
    12601 ttacaatctt tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    12661 caaaatatga ggaactattt ttccatttgt ttctctaata ctcaggtcaa aacgctgatt
    12721 tcacaacctc ttgataaacg gtgttcaaac ggctgcttct cggtaataag cccactagcc
    12781 tagaaaatat gaaaagctat tttctaacta gcgttcttat tactcaaagc ataacgctga
    12841 tttcacaacc tcttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    12901 cggcaaaaca tgaggaacta tttttccgtt tctttctcta atactcaggt cagaacgctg
    12961 atttcacaac ctctctttca caaccttatt ttactcgttt ataattttca tcgaagtatt
    13021 ttccgctacg gatatcatct ggcattccat cgtatggttc ttcgctaata acatcttcat
    13081 tgtctatacc tgcatcaccc atataagtga tcgtagcaaa ttcaggaaca agtaattttg
    13141 gataagtttc atatttttca cgagctgctt ccatttctgc gatatgttcg ttttggaaag
    13201 taacagtgat ttctggatgt tcttcaaccc atttagggaa aaagatcatt ccgtgaagtt
    13261 tcttttcatt tggcataaag tccaatgcca cgtctgtccc tgttggctct gtccaagtga
    13321 ttttgaatac gccgtccgtc aatttaacga tatcagcttc ttggtctttt acccaacggc
    13381 ctttgaccat tccgccatgg atacggtaat caacagtatg gtcatttttg gcataccatt
    13441 catattccca accattgtca tacgtataga taaagtgagt tccaataaaa tcttccaatt
    13501 ctttaaattc tttcatgttt aagcatctcc ttataaatta aaacatgtaa taaactacat
    13561 gttttcgatt actcatttat gatagaatgg atttaaagat ttgtcaagga gggaaaactg
    13621 atggcaagaa aaaatacact tcgatatatt ttgttagggt tattaagtaa gaagaagatg
    13681 acaggatatg aattgaatca atcgttcaaa aatgaaatag gagaattttg gcaagcaaaa
    13741 catagtcaga tttatcctga acttgcaaaa atggaagagc aaggaatcat tcagcatcaa
    13801 gtagagatca caggtgaaaa attggaaaag aaagtttatg aattaacaga agaagggaag
    13861 gaacaattgg cagagtggat tcatacaccc accaatgaac taccggttaa tcgagacgaa
    13921 tttgttctga aattgtattt cgtcaaagac gtgaatgatc cggcattagt agaaattatt
    13981 cagcagcagc gtgaccttca tgaagaaaaa cgacttcatc tattagagcg aaaaaaaacg
    14041 atctttccta ctgaaaaaga aaaacatgaa aattacggac actacctgat tttagcgcat
    14101 gcgatcaatc gggaaacaga atatgcaaat tggctaacag atgtattaga agaaagtaac
    14161 cgaaaaaaat aaaaaaatat ccgaacagtg atggagaatt cctttctata tgaaagaaac
    14221 gcagatcacc gttcggatat tcttatgttc aaacaggcct tttttcaagt atttatgtag
    14281 catctactta ttaaaaagct tggtacttag gagattagtg gcgtttagag ttcttttaac
    14341 aactgttcgt agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa ttcgtctgtc
    14401 tcgttgtcat gaatgatgta catcttagca ttctcttctt tgaagttacc gatttgtaaa
    14461 atcgtatagc gttcgtcgat caattcgata tctttgattg cttttgaaaa aactttacgg
    14521 acatggtgca ttttcttttg gcggatttct gccttttctg cagcttttga caaagtaaat
    14581 ccgtcatcta aatatttttt gatcagttct acttttatga ctgtaggtaa acgatatttt
    14641 ctaggacaat tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc ccaatacctt
    14701 aattgacgtg gagacacacc tacgatatca cttaattcac tgatgccaac aaccaattga
    14761 tcattttcca acaaacgttt aagggaagcc ccaaccattt ttgtcactcc ttttgtcctc
    14821 ttgcttccct taattatatc cgtattaact tatgtgtcaa gtaggttgac aaatagcgaa
    14881 tttaggacta aagtattcta gtagtttcag aaaaaattca gaaagaagga gaaatatgag
    14941 taacaaccaa cccgtagata tttacggaaa accatataat cggacacttt tagttgttgt
    15001 cttattaatt ggtacatttt gtaccgtttt gaatcagacc ttattgacga cagcttttcc
    15061 tacattgatg aaagctttcg atatttcagc gtctgatgtg caatggttaa cgactggttt
    15121 cttgctagtc aacgggatca tgatcccaat aacagcatgg ctgatcaata aattcagttc
    15181 aagaaattta tatctttctg ccatgacgat ctttttgatc ggaacgatta cttgctttac
    15241 agcacctaat tttagtacat tactaatcgg tcgtttgatc caagcttgcg gtgtgggagt
    15301 atcgatgcca ttattgcaaa atatcatgct atctattttc ccgccagaaa aaagaggatc
    15361 agcaatgggg atgtcaggga tcgttattgg acttgctcct gctttaggcc ctacattatc
    15421 tggttatatc attgataact accattggcg tgacttgttt ggtatggtac tgccaatcgt
    15481 tgttcttgtc ttggttttag cattcttcct aatgaaaagt gttattccat tgtcaaatcc
    15541 tagcattgat attttatccg ctattttatc aacgattgga ttcggtagtt tactttacgg
    15601 attttctagt gtaggagatg acggctgggg aagcttgaaa gtaattggtt tcttagttgt
    15661 tggtgtgatt ttcatcgcct tgttctcatg gcgtcaatta catttggaac atccattctt
    15721 ggaacttcgt gtattcaaat catctacatt cacgattgca gctgttttag ctggtgtaac
    15781 aaatatggcg atggtagggg cagaaatggt cgtaccattg tatattcaaa atattcgtgg
    15841 agaatcagca ttccattctg gcttgatgtt gttgccgggt gcattgatca tgggggctat
    15901 gatgcctgtg acgggtgcga ttttcgataa atacggcgcg aaacgtttag ccatcacagg
    15961 gatgttcatt ttgacagcgg caactgcacc atttgctttc ttgacaaaaa cgactccagt
    16021 tttatatatc gtgatccttt atgcgatccg tatgtttggg atctccatgg tcatgatgcc
    16081 ggtaacgact tctggtatga atgcattgcc aaataacttg atcagtcatg ggacagcggt
    16141 aaataataca ttccgtcaaa tcgcaagctc gattggtaca gctattttga tcagtgtgtt
    16201 gacgaatgtg acaaaagcaa atatcccaag caaatctgta ttggataata cgccattagc
    16261 ttataaagat caggcaatca atgccacact gtcaggttac catgccgcgt ttatcgtagc
    16321 gatcatcttt gggattcttg gctttattat tgccttttta ttgaagaaaa aagaacctgt
    16381 tctaatgaaa gcgggggata atgcatgatt attggagttt tgattgtcag cttgcttgct
    16441 tttgctttaa cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc tttgattttc
    16501 ggactaatct tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa tcactttgga
    16561 atggaaaaag taacggaaac aaaaacagaa aaaattgttt ccagtgctga ttcccaagga
    16621 gcagatatgc ttttgtataa agctttaggg aatggaaaag aaaaagtgta tctttaccga
    16681 acaaatgaaa aacaagaaaa accaaaagca actggtacgg ataacgaaac aaataaagta
    16741 gaaaagacag acggcgatgc tgaaaaagtg acgaaaacta cttattggga atacaaaaac
    16801 gacatgtata aattctggtt caatattgct gataacaatc acgaatatga cagccgagtg
    16861 aatacattca aaatcccaga gacatgggta gaattaagta cggaccaagc agccaagtta
    16921 gctgaattag tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc ttatgttcaa
    16981 gacggtatgg taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga gcaagaacaa
    17041 cgtacgaaag ccttagctgc tgaattccaa caacaagcat tcgcaaaatt agtgaaagaa
    17101 gcaaaaggcg aataatattt tgaacaccat gtaggaggtt gtgaaaaagc tgctctgcat
    17161 caagcagtaa gaacaatcag ataaaaatag cactccatat ttttcatctg attgggctta
    17221 gtgccgtaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta
    17281 actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg actattgtga
    17341 cttgtgaccg aggagttggc aggagaacac cgtttattcg aaaaaaggaa ctgcgacaag
    17401 acttttgtca cagttccttc ttttttttgc tatgctatac tagatgtaaa aaataggaga
    17461 aataaaatga gggaaattta ttcttttgaa tcaaaaatct atgcttccga agtcggcaag
    17521 ggcggggcat atattatttt tccgtatgat ataagaaaag aattcggtgc tggaagagtt
    17581 aaagtccaag caacttttga caatgtgccg tatgaaggca gtatcgtgaa tatgggcgtg
    17641 aaaaatccag atggttcagt ctgctacatc cttggactac gaaaagatat tcgtaaaaac
    17701 atcggaaaag aaatcggtga tattgtagca gttacagtga agcaaaaata aaagaggttg
    17761 tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc aaaagccagt ttctcggtag
    17821 taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac aaaatttttc ttactactcg
    17881 aaacgaagcg gctttatcac aacctcttga taaacggcgt tcaatcagct gtccctcggt
    17941 attagctcaa aaacggcaaa atatgagaag ctatttttcc atttttttct ctcatactca
    18001 ggtcataacg ctgatttcac aacctcttct ttcataatgg tttttcacca tctttataat
    18061 aaactggttc tttgatatgg aaatctattt tttccaacca atctaaaatc ttgtaaccta
    18121 actcttctga catcggaaaa gaggcgttga tttgtgcatt ggagatatgg tcaacatgga
    18181 taccggaagt gatcgtacaa cttcccggaa cggcagtttt gatgatcaca gctatttttt
    18241 cggctaagac atcgtcttta tggaaccgac catcgtggct agggaaacgt acagtttgca
    18301 tctctgtatc acgagaaaat gtagtgactg ttccgatatg tggtgtatct ccgcctgtta
    18361 aagtgatcaa taagtcttta ccgattactt taacagcagc ttggatcgtg aaacctagtt
    18421 tggttacttc aactatcgtt tctttttcca tattaatcac cctgccaacg ttttgaatgc
    18481 tcatttacaa tttcaaattg atccaaaagt ttcattgtat ggtgattaac aagatcttgg
    18541 atcgtttgag gctgattata aaaagcagga attggtggta tgatttgcac acctagtcgt
    18601 gctaatttag tcaagttttc caagtgaatg gcatttaatg gcgtttcacg aggaacaatg
    18661 actaactttc gttgctcctt cattgttaca tcagcagcac gtgcaatcag gttgtcgcct
    18721 aaaccaacag agatacctgc gactgttttc atacttgccg gtacgatgat cattccatcg
    18781 gtcaaaaatg atccgctggc gacagcagca cctaaatctt tgtttgaata atggtaatca
    18841 aacaggcttt cgaattcagt tagtgataag tcggattcca gctttaagtt ctgttttgcc
    18901 catggactga tgattccgtg tgtttctatc atcggatatt cttttagttt cttcactagg
    18961 ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa tcacgatttt tttcttccgt
    19021 tgtgccaatt gttccatttt ccgcactcct ttttatgatg tcaaaacagt tgcagatcat
    19081 tcattatttc aaatattttt cccaatcttc tacttcttgg aattttgcac gtacaaaatg
    19141 ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt tttgcactta tcccacggaa
    19201 acgaatagag ccaggattat attctggtaa ttctgatggg tccaatggat ggttcctcat
    19261 tcctgggatc acgatgatat ctttatctcc ttggaaacga gtgttgaaag tccaaactac
    19321 atcgttcatg tcgaaaatat caacatcttc atcgactaaa atgactgtct tcaattcttt
    19381 gaaggctgat aaagcaagaa tagctgcttg acgctgaata ccttcatccg cttcgttttc
    19441 tttacggatt tgcatgatgg tcatcaattt accaccacct gcaggaggat tgtagacatt
    19501 tactactttt cctggaatag ctttatcgac caaggcaaga atacttgctt ctgtaggaat
    19561 accggccata gatacgtgtt cttcactggg accaatcgtt gtttgcatga ttggattgtc
    19621 ttttcggtga gtaactgctt tgactttcac tacttgtaca gctggattag catccccagt
    19681 atagccagga aattctggca ttgcttttcc tgagttcgtg ttgatatctt cttgcattgt
    19741 ttcatttggc atgatttctg cttcaatgat gaattcagca cgagcaatac ccacttcatc
    19801 gactgctata ccatcaacga tttgaatagg ttcgttacgc aaagctcctg cacaccatag
    19861 ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc gtgccaatgg caattgctgg
    19921 atctaaacca atattgatag tgataggcat tggtttattc aaggcttcga attgtttttg
    19981 gaagtgacca atgtggcgtc cgccaggcat gatgtacata ccgatcgtat ctttatcttc
    20041 tagaaccata cgatggatcg ttacatcgct cattgttttt tctggatctg atcctaagac
    20101 tacacccatt gtgatgtatg gaccagcatc ttcaggagta tttgtaggtg caggcaaaat
    20161 gttgcgaata tcgaagtctg catcggtagc tagatgaacg acttcttgtg ctggagcgtc
    20221 tgctttgtct actttcacag gtttgattgg attttctaca gcgtctttca agaaacgacc
    20281 aagtgttcga taatcatggt gcaaaatttc acctacacgt ttacgactag ccattgttcc
    20341 gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg aaagtcatag ctggtccttc
    20401 ttgagtcgga cgttttactg taccgccagc accgatataa cggtaaacac cagataattc
    20461 tgcttcagga tcgatttcaa tatctgtttc gtgatattga cctggaagtg tttttagttc
    20521 ttcgatgact tttcttaaat catacggttg atctgtcatg agagattcct ccttagttgt
    20581 ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca attcaacttg ttcggtaaag
    20641 tattctacaa tggaatggat gcgttttttt gataatagaa taaactatga tatacttatc
    20701 actaaaagaa agcgcatact tagtgttgga ggaaggaaag tgaaaaaaat ggaggagtca
    20761 tttcctaaag cagtaaaagt ggaaaatata gcgaatattt tgaaagttac atttgaaaac
    20821 ggagaagtaa aatacgtgaa atctcattgg acagaagaga taaccgatgc cttgcaattt
    20881 ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac tttctcgtaa tatgtggatt
    20941 gggactgaag taacgattga agcagatgga acagtattca taaatggaaa agacaggtat
    21001 acgccagaag agttgtggta caaaggaaag aaaagtatac cagaattata aaaaataatt
    21061 aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg tttcttgctg ttttctacta
    21121 tttcagcgct cttatgactc aaatctaaat gtggttgtca caaccttatt ctgcacgcaa
    21181 agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga agatgaccgc caagcatcgt
    21241 caatatagta gagacgatca ctagaataat cgcatggaca ggatttagtt gagcaacatt
    21301 ttttagatcc gtcatgttgt ataagatgct attgataggg aacgtagcta gccatgcaat
    21361 aaacacacct aatatcccag aagaaatacc taaaatacac gtttctgcgt caaatacgcg
    21421 agtgatgtct tttttacgtg cacctaaagc ttttagtaca ccgatttctt ttgtccgttc
    21481 aatcacagat gtataagtaa tgatactgat catgatcata cttgttacta aagagatccc
    21541 tgcaaaagca ataaggacat aagtgatagc atccatcaat ccgccagtca gttcagtcat
    21601 cgttccagct aaatcagtat aaatgatttt atcttcatct gatttgcctt tattgtaatc
    21661 atctagataa tccaagatct tttctttgtc ttcaaaattg ttcggataaa tcatgatact
    21721 tgaaggcaag ctgtctccac ctaaataact aagcaatgtt tgtttggcat tttcgtcaac
    21781 tttttcagta gttaagacat ttacaccact gtctctttga gctttgacga tttctgattc
    21841 tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta taagcgattc ctggagataa
    21901 gagattcatt gtagaagaag atttcacgcg taggatcccc gagatcttca attcatcaga
    21961 agcattctga tacatcgcat cataatctgt attagggata aaattcccag ttggaagttt
    22021 tgtatagtaa gtattgttgt ttactaattt gaatgttgta ccaacgatat catcaaaatc
    22081 aagtttttcg tcttctttca catcaaatcc aagattcttc aatgcgttga tattagtatt
    22141 attattgccg tctacgatca gcacgacatc tgttgctgag gctggataac ttccggcaag
    22201 taaagaataa ttgtctttta aaaagttttc tttgctagtg tcaagctgtg tagggaaact
    22261 tgacactccc acacctgtca ttgctgacat cgtactagat aaagaaagag attccgtatc
    22321 aggattttga ttcgagaagc tgacaggctg tactttgcca tttacatcac gtaaaagatt
    22381 gattcctgta gtacgggtaa atccgatatt attacttaag ttaggatcga tgtctgttac
    22441 gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt gctttatctt cttcgctgac
    22501 ttttgcagta atcgttttgc tgtctggata atccgctttg cttgcaccca atccggcatc
    22561 gtcacgagtc tggttagtcg ttaccttaga aatagtaatc ggaaatttcg ccatcgtttc
    22621 agcttgcgtg ttatcgattt gtttttggaa tccagaagaa agggacagaa cgatcgcgat
    22681 tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa gtacgtcctt tttttgttcg
    22741 gatattgttg aaggaaagct ttaacgctgt ccaaaaactc atctttgttc tgcgtaagtt
    22801 aaattgatca tcttttggtc gttcgatgtg aggatgggaa tctgttaaga tcttaccatc
    22861 agaaaattca atgattcgat ctgcgtattg atgggccagt tcaggattat gtgtgaccat
    22921 gatcactaat ttttctttcg ataactcttg gatcaattcc atgatctgga tacttgtttc
    22981 tgtatctaac gctccggttg gttcatcgca caaaaggata tctgggtcat tggctagtgc
    23041 tcgagcgatg gccactcgct gcatttgtcc accagataac tgatttggct ttttatgcat
    23101 atgatttgtc aagccgactc ggtgcaatgc atcttctgcc cgttttcgtt tttcgtcttt
    23161 agaaacacca cttaatgtca tcccaagttc aacgttttca ataatgccta gatgaccaat
    23221 caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt cggtaggcat cccagtcact
    23281 gtccttaaag tctttagtag actttccgtt gattaccata tcaccggaat cataattatc
    23341 tagtccacca atcacattca gcatagtcgt tttaccagaa ccactaggac ctaaaatcgc
    23401 tacgaattct ttttgtctga aagcgacaga cacaccatcc aatgctttgg tggtggtttc
    23461 gccgacttta taatattttt ttatatcttt taattgaagc ataatcaccc ttctcccgta
    23521 ttgaaatatt cggtatacta ataagtatat cagaacaatg atcgtagtat acgaaataaa
    23581 tgttaactga agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa cagagggaga
    23641 tcaatgaatc gtattttagt agtagaagat gatccgcaat tacagttgct ttaccgttct
    23701 gtgttggaac gagcgggctt ttcagtcatc accgcattga atggagagga tgcgctgaaa
    23761 aaactagaag cgacacgtat agaattaatc attacagata tcatgatgcc gaaaatggat
    23821 ggctatgact tgctagactc attacgtagc accagatttg acacacctgt tctaatgatt
    23881 acggcaaagg ccgattttga ggataagaaa cgcgggttca aattaggagc agatgattat
    23941 atgacaaaac ccatcgatgt caatgagatg atcctacgtg tggaagcatt gcttcgacgt
    24001 tcaaagatca attacagcag tagcttgata atcggacaaa caaaattaga gctagatgct
    24061 tatacagtgt tacaagatca gcattcgttg attctgcctc aaaaagaatt tctgcttctg
    24121 tataaattac tgtcttatcc caacaagatc ttcactcgcc aacagttgat ggatgatatt
    24181 tggggattag atacgaatac agaagaacga acgatcgatg tacatatcaa acgtttaagg
    24241 acccgttttg aaaagacgga agattttcaa atcatcacag tacggggact aggctataag
    24301 gcggttattt aaagatgaaa aaaaagctat tttcacaatt atggatctat tttgctagta
    24361 ttgtcttcat ttcgatctta gtgacactgc tgtgttttct cggttttatt ttcttattgt
    24421 ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc aacgttatac ccgttattcg
    24481 tctttgcggg gttcagtatg gtcgtgggta caggaatatc gatttttgtt gggcggcgta
    24541 tcctgcatcc aatcagtgcg ctagggacaa atatgagtct ggtagcaacg ggagattttt
    24601 cgattcgaat ggatgaacag cagaaagtag cagaagtaca gcaactgtat aaagatttca
    24661 acgttatggt ccaagaactc aatagtatcg agacactgag gaatgacttt gtgtccagtg
    24721 tatcccatga attcaaaaca ccactagcaa caatccaagg atatgttcaa ttgttacaag
    24781 ccccaaatct ctctgacgaa gaacgccaaa tatttttata tcggattatc gaaagtatca
    24841 cgcagctttc acaattaaca gagaacatct taaaattgaa caaactggaa aaccagcgta
    24901 tccaactgga aaagaaagag tatcgattag atgaacaaat cagagaagta atcgtttttt
    24961 ttcagcctaa atgggagaaa gaacagctag aattggatat cgaacttgct gcagtaaatt
    25021 acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa tatcatggat aacgccataa
    25081 agtacaatca aatcaatgga cagattcata tcaaactatt tgagacagcc acagagattg
    25141 tactggaagt gaccgattca ggtgtaggaa tgaatgaaga gaccagagat cgaatgtttg
    25201 aaaaatttta tcaaggagat acaagtcgtc aaatttctgg gaatgggcta ggactttcac
    25261 tggttaaaaa aattctagaa ctgcatgatg gacgtattga ttatagcagt atcgaaggag
    25321 taggaacgac agcaatgata cggttaagta aacaatagga aaaagaataa aacccctaaa
    25381 aattctattg aaggttttta ggggttttta gtcgtttttt tgtgattagg gaaatgttct
    25441 tacggttctt ttggcataaa a
//
LOCUS       DAFEZB010000039        24395 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFEZB010000039
VERSION     DAFEZB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.039"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            115..696
                     /locus_tag="LP859_001613"
                     /pseudo
     CDS             115..696
                     /locus_tag="LP859_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            700..1341
                     /locus_tag="LP859_001614"
     CDS             700..1341
                     /locus_tag="LP859_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029574.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1338..2255
                     /locus_tag="LP859_001615"
     CDS             1338..2255
                     /locus_tag="LP859_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3029575.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2252..2923
                     /locus_tag="LP859_001616"
     CDS             2252..2923
                     /locus_tag="LP859_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029576.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3464..3952)
                     /locus_tag="LP859_001617"
     CDS             complement(3464..3952)
                     /locus_tag="LP859_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL3029577.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4153..4419)
                     /locus_tag="LP859_001618"
     CDS             complement(4153..4419)
                     /locus_tag="LP859_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL3029578.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4419..4682)
                     /locus_tag="LP859_001619"
     CDS             complement(4419..4682)
                     /locus_tag="LP859_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3029579.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5158..5343
                     /locus_tag="LP859_001620"
     CDS             5158..5343
                     /locus_tag="LP859_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3029580.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5485..7308)
                     /locus_tag="LP859_001621"
     CDS             complement(5485..7308)
                     /locus_tag="LP859_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL3029581.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7906..10611)
                     /locus_tag="LP859_001622"
     CDS             complement(7906..10611)
                     /locus_tag="LP859_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL3029582.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10917..11864)
                     /locus_tag="LP859_001623"
     CDS             complement(10917..11864)
                     /locus_tag="LP859_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL3029583.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            12054..12323
                     /gene="rpsN"
                     /locus_tag="LP859_001624"
     CDS             12054..12323
                     /gene="rpsN"
                     /locus_tag="LP859_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL3029584.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12499..12861)
                     /locus_tag="LP859_001625"
     CDS             complement(12499..12861)
                     /locus_tag="LP859_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3029585.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12872..13993)
                     /locus_tag="LP859_001626"
     CDS             complement(12872..13993)
                     /locus_tag="LP859_001626"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL3029586.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(14009..14359)
                     /locus_tag="LP859_001627"
     CDS             complement(14009..14359)
                     /locus_tag="LP859_001627"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL3029587.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14489..16000)
                     /locus_tag="LP859_001628"
     CDS             complement(14489..16000)
                     /locus_tag="LP859_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL3029588.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16322..17674)
                     /locus_tag="LP859_001629"
     CDS             complement(16322..17674)
                     /locus_tag="LP859_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL3029589.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17827..18903)
                     /locus_tag="LP859_001630"
     CDS             complement(17827..18903)
                     /locus_tag="LP859_001630"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL3029590.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            19099..20844
                     /locus_tag="LP859_001631"
     CDS             19099..20844
                     /locus_tag="LP859_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL3029591.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21124..21561)
                     /locus_tag="LP859_001632"
     CDS             complement(21124..21561)
                     /locus_tag="LP859_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL3029592.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21545..22918)
                     /locus_tag="LP859_001633"
     CDS             complement(21545..22918)
                     /locus_tag="LP859_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029593.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23240..23728)
                     /locus_tag="LP859_001634"
     CDS             complement(23240..23728)
                     /locus_tag="LP859_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029594.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23750..24352)
                     /locus_tag="LP859_001635"
     CDS             complement(23750..24352)
                     /locus_tag="LP859_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL3029595.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6997 a         5196 c         4511 g         7691 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
      181 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
      241 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
      301 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
      361 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
      421 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
      481 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
      541 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
      601 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
      661 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
      721 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
      781 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
      841 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
      901 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
      961 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
     1021 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
     1081 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
     1141 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
     1201 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
     1261 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
     1321 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
     1381 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
     1441 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
     1501 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
     1561 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
     1621 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
     1681 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat ggttttgaaa
     1741 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
     1801 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
     1861 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
     1921 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
     1981 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
     2041 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
     2101 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
     2161 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
     2221 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
     2281 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
     2341 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
     2401 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
     2461 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
     2521 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
     2581 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
     2641 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
     2701 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
     2761 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
     2821 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
     2881 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
     2941 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
     3001 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
     3061 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
     3121 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
     3181 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
     3241 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
     3301 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
     3361 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
     3421 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
     3481 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
     3541 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
     3601 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
     3661 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
     3721 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
     3781 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
     3841 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
     3901 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
     3961 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
     4021 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
     4081 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
     4141 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
     4201 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
     4261 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
     4321 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
     4381 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
     4441 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
     4501 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
     4561 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
     4621 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
     4681 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
     4741 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
     4801 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
     4861 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
     4921 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
     4981 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
     5041 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
     5101 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
     5161 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
     5221 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
     5281 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
     5341 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
     5401 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
     5461 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
     5521 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
     5581 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
     5641 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
     5701 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
     5761 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
     5821 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
     5881 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
     5941 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
     6001 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
     6061 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
     6121 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
     6181 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
     6241 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
     6301 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
     6361 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
     6421 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
     6481 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
     6541 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
     6601 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
     6661 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
     6721 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
     6781 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
     6841 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
     6901 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
     6961 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
     7021 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
     7081 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
     7141 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
     7201 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
     7261 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
     7321 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
     7381 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
     7441 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
     7501 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
     7561 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
     7621 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
     7681 catctgcggt aataagctca accaaattaa aaacataaga agctgttttt atttggtcgt
     7741 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
     7801 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
     7861 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
     7921 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
     7981 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
     8041 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
     8101 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
     8161 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
     8221 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
     8281 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
     8341 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
     8401 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
     8461 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
     8521 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
     8581 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
     8641 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
     8701 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
     8761 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
     8821 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
     8881 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
     8941 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
     9001 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
     9061 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
     9121 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
     9181 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
     9241 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
     9301 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
     9361 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
     9421 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
     9481 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
     9541 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
     9601 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
     9661 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
     9721 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
     9781 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
     9841 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
     9901 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
     9961 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    10021 aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt
    10081 caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact
    10141 tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc
    10201 gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt
    10261 tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt
    10321 tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc
    10381 cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat
    10441 tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata
    10501 tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat
    10561 ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc
    10621 ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct
    10681 tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta
    10741 catattgaga aataattaag gattacagcc tttactttat catgaaataa aagggaaaga
    10801 taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt
    10861 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
    10921 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
    10981 tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
    11041 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
    11101 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
    11161 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
    11221 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
    11281 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
    11341 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
    11401 accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
    11461 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
    11521 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
    11581 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
    11641 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
    11701 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
    11761 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
    11821 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
    11881 tttctttaag tttacgttta tgtctttatt ttgacaaaaa aaatgtcttt tcgttttggc
    11941 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
    12001 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
    12061 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
    12121 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg
    12181 ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc
    12241 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
    12301 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
    12361 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
    12421 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
    12481 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
    12541 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
    12601 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
    12661 tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc
    12721 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
    12781 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
    12841 aatatctcct ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct
    12901 gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca
    12961 tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt
    13021 cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt
    13081 tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga
    13141 tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc
    13201 cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct
    13261 gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc
    13321 cataacgaat catattgccg attgtttcat catgccataa agccgttgca ctattgctga
    13381 catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt
    13441 gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt
    13501 cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg
    13561 tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct
    13621 ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag
    13681 ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga
    13741 taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa
    13801 cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac
    13861 cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt
    13921 catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc
    13981 aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg
    14041 tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa
    14101 caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc
    14161 cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta
    14221 cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg
    14281 caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga
    14341 tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct
    14401 accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc
    14461 ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc
    14521 gttttttatc attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac
    14581 cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc
    14641 caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg
    14701 tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa
    14761 ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta
    14821 gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga
    14881 atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa
    14941 tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc
    15001 cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat
    15061 agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta
    15121 caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag
    15181 acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt
    15241 cgtctacacg gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa
    15301 acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga
    15361 tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt
    15421 taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt
    15481 gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt
    15541 catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa
    15601 tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac
    15661 ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc
    15721 gccctaaacg atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa
    15781 ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg
    15841 cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta
    15901 gtggaatcgt ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt
    15961 tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt
    16021 gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt
    16081 ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga
    16141 aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc
    16201 attattatga catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct
    16261 tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt
    16321 tttatttcat ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt
    16381 ttagcataat actatctgtt gctgcaagat tttgtttgat agcatcaatc aatttattct
    16441 tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta
    16501 gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt
    16561 cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca
    16621 cagcgattct gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg
    16681 ctgttggatt tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc
    16741 attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg
    16801 acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc
    16861 caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt
    16921 ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta
    16981 ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt
    17041 ctgatacaag tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa
    17101 gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa
    17161 gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg
    17221 tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt
    17281 gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg
    17341 ttttaccatt gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg
    17401 ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg
    17461 gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct
    17521 ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta
    17581 tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca
    17641 cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg
    17701 aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc
    17761 tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat
    17821 ttctctttat tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa
    17881 ctggatcagt tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg
    17941 gtacatactg aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt
    18001 tttatttgtc aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc
    18061 taacttcgcg tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg
    18121 catttcgatt ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac
    18181 gacttcacca ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc
    18241 gcgcgcttca attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa
    18301 agcattttgc agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt
    18361 cgccggtttg acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt
    18421 aggattttct ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc
    18481 agcttgtaaa atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc
    18541 tgcgccgaca taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc
    18601 gtttggccca tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt
    18661 gatcactttt cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa
    18721 gacatctttg ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt
    18781 tgtaataaat acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa
    18841 tgcggaaaga atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt
    18901 caaaagattg tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca
    18961 aaacaaaaat aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgag
    19021 cgtcatttat tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa
    19081 agaaaaggag ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt
    19141 cgagtgttca tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag
    19201 gtaaaaaaag cctttgaaga actaggcccc accttcatca agataggaca gatgctttct
    19261 gttcgagaag atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt
    19321 gtacctagcg acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca
    19381 ttatcagata tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag
    19441 gctcatcgtg ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac
    19501 atcgccgaag agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa
    19561 tacattcctg aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc
    19621 ttgatcaatg aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag
    19681 aacaatggct ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa
    19741 gtgatcgtga tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac
    19801 aaaactgaaa cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag
    19861 cttttagttg agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct
    19921 catccaggga atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga
    19981 aaaactgaaa cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct
    20041 gcagaagacc ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat
    20101 ctttctgcag gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat
    20161 gcttatcgaa tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa
    20221 agtcggttcc accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat
    20281 gaaatcaact tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg
    20341 cagttcgatc gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc
    20401 cgcgtcctcg atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat
    20461 tttctcactc atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa
    20521 gggatgaagg ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact
    20581 tcgggacaag gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc
    20641 gaaagtatga tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc
    20701 tccctgcttg tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata
    20761 tttacgtatg ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa
    20821 atgtataaga agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa
    20881 taagaagggg ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc
    20941 gaagaggcta cttctgaaac accgagaata aaaaatgtga aaaagctgtt ctgcacccag
    21001 taataagaac aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt
    21061 ttgatgtatt cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa
    21121 accttagtca attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa
    21181 gggttttgga ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg
    21241 ttctagaagc gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg
    21301 cacacccttc ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt
    21361 cttcttaacc ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac
    21421 gatttggaca tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac
    21481 cacgtcaaga ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc
    21541 tccatcaatg aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta
    21601 atccgctcag taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt
    21661 ctatcggcac tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt
    21721 cttttgctaa aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat
    21781 tttgagtagt atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat
    21841 aatattcctt atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac
    21901 ctatcgcaaa accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt
    21961 gcatagccaa tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg
    22021 caatcaagcc tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca
    22081 gtatgccaac ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa
    22141 aagtaaacag atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa
    22201 acacgaacaa gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat
    22261 cgaataacaa tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa
    22321 caagctctcg cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg
    22381 gataatgact aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg
    22441 ctgcaatctg agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag
    22501 ctgcaaataa gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat
    22561 tgaccgtggt attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa
    22621 aataaaataa aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa
    22681 tcagcagagc gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta
    22741 aaaatagtcc agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat
    22801 tatgcgagtc tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta
    22861 agataaaggg cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga
    22921 caatggctcc ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga
    22981 aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct
    23041 tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt
    23101 cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct
    23161 ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac
    23221 tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa
    23281 atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa
    23341 cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt
    23401 gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt
    23461 tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt
    23521 aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt
    23581 tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa
    23641 tgatcggcac aaaagcttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt
    23701 cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac
    23761 cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc gaccatggtt
    23821 tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta
    23881 gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa
    23941 tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa
    24001 taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa
    24061 tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg
    24121 ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa
    24181 caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg
    24241 atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa
    24301 taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc
    24361 cccacatctt ttttcattta ttaaaactgc ctcct
//
LOCUS       DAFEZB010000040        23827 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFEZB010000040
VERSION     DAFEZB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.040"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(53..787)
                     /locus_tag="LP859_001636"
     CDS             complement(53..787)
                     /locus_tag="LP859_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL3029596.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="LP859_001637"
     CDS             1114..1524
                     /locus_tag="LP859_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL3029597.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="LP859_001638"
     CDS             1517..2218
                     /locus_tag="LP859_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL3029598.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="LP859_001639"
     CDS             2218..3804
                     /locus_tag="LP859_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029599.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="LP859_001640"
     CDS             3823..4044
                     /locus_tag="LP859_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL3029600.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="LP859_001641"
     CDS             4041..4802
                     /locus_tag="LP859_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL3029601.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="LP859_001642"
     CDS             5182..5691
                     /locus_tag="LP859_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL3029602.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="LP859_001643"
     CDS             complement(5766..6305)
                     /locus_tag="LP859_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL3029603.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LP859_001644"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LP859_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL3029604.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="LP859_001645"
     CDS             7318..8169
                     /locus_tag="LP859_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL3029605.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="LP859_001646"
     CDS             8197..8832
                     /locus_tag="LP859_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL3029606.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="LP859_001647"
     CDS             8852..9625
                     /locus_tag="LP859_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3029607.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="LP859_001648"
     CDS             10033..10830
                     /locus_tag="LP859_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL3029608.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="LP859_001649"
     CDS             10808..11221
                     /locus_tag="LP859_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL3029609.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="LP859_001650"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="LP859_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL3029610.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="LP859_001651"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="LP859_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL3029611.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="LP859_001652"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="LP859_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL3029612.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LP859_001653"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LP859_001653"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL3029613.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="LP859_001654"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="LP859_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL3029614.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="LP859_001655"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="LP859_001655"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL3029615.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="LP859_001656"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="LP859_001656"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL3029616.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="LP859_001657"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="LP859_001657"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL3029617.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="LP859_001658"
     CDS             21905..22483
                     /locus_tag="LP859_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3029618.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="LP859_001659"
     CDS             22535..23017
                     /locus_tag="LP859_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL3029619.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="LP859_001660"
     CDS             23134..23508
                     /locus_tag="LP859_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029620.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DAFEZB010000041        23571 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFEZB010000041
VERSION     DAFEZB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.041"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP859_001661"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP859_001661"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL3029621.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LP859_001662"
     CDS             complement(1172..1867)
                     /locus_tag="LP859_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL3029622.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LP859_001663"
     CDS             complement(1880..2596)
                     /locus_tag="LP859_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL3029623.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="LP859_001664"
     CDS             2926..3531
                     /locus_tag="LP859_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL3029624.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="LP859_001665"
     CDS             3856..4179
                     /locus_tag="LP859_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL3029625.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="LP859_001666"
     CDS             4361..5731
                     /locus_tag="LP859_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL3029626.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="LP859_001667"
     CDS             complement(5861..7162)
                     /locus_tag="LP859_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL3029627.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="LP859_001668"
     CDS             complement(7392..9350)
                     /locus_tag="LP859_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL3029628.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="LP859_001669"
     CDS             complement(9527..9793)
                     /locus_tag="LP859_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3029629.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LP859_001670"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LP859_001670"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL3029630.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LP859_001671"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LP859_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL3029631.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="LP859_001672"
     CDS             13137..13700
                     /locus_tag="LP859_001672"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL3029632.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="LP859_001673"
     CDS             complement(13953..14474)
                     /locus_tag="LP859_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL3029633.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="LP859_001674"
     CDS             complement(14477..15319)
                     /locus_tag="LP859_001674"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL3029634.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="LP859_001675"
     CDS             complement(15312..16037)
                     /locus_tag="LP859_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL3029635.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="LP859_001676"
     CDS             16213..16509
                     /locus_tag="LP859_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029636.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="LP859_001677"
     CDS             17077..18669
                     /locus_tag="LP859_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3029637.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="LP859_001678"
     CDS             18682..19422
                     /locus_tag="LP859_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3029638.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="LP859_001679"
     CDS             19590..20939
                     /locus_tag="LP859_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3029639.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="LP859_001680"
     CDS             complement(21067..21657)
                     /locus_tag="LP859_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL3029640.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="LP859_001681"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="LP859_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL3029641.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DAFEZB010000042        22852 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFEZB010000042
VERSION     DAFEZB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.042"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(99..1331)
                     /locus_tag="LP859_001682"
     CDS             complement(99..1331)
                     /locus_tag="LP859_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL3029642.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1586..2155
                     /locus_tag="LP859_001683"
     CDS             1586..2155
                     /locus_tag="LP859_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3029643.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2333..2773)
                     /locus_tag="LP859_001684"
     CDS             complement(2333..2773)
                     /locus_tag="LP859_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL3029644.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2931..3695
                     /gene="map"
                     /locus_tag="LP859_001685"
     CDS             2931..3695
                     /gene="map"
                     /locus_tag="LP859_001685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL3029645.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3727..4650
                     /locus_tag="LP859_001686"
     CDS             3727..4650
                     /locus_tag="LP859_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL3029646.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4726..5865
                     /locus_tag="LP859_001687"
     CDS             4726..5865
                     /locus_tag="LP859_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL3029647.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5858..6658
                     /locus_tag="LP859_001688"
     CDS             5858..6658
                     /locus_tag="LP859_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3029648.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6658..7485
                     /locus_tag="LP859_001689"
     CDS             6658..7485
                     /locus_tag="LP859_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3029649.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7463..8197
                     /locus_tag="LP859_001690"
     CDS             7463..8197
                     /locus_tag="LP859_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3029650.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8297..9163
                     /gene="rfbA"
                     /locus_tag="LP859_001691"
     CDS             8297..9163
                     /gene="rfbA"
                     /locus_tag="LP859_001691"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL3029651.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9177..9749
                     /gene="rfbC"
                     /locus_tag="LP859_001692"
     CDS             9177..9749
                     /gene="rfbC"
                     /locus_tag="LP859_001692"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL3029652.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9771..10799
                     /gene="rfbB"
                     /locus_tag="LP859_001693"
     CDS             9771..10799
                     /gene="rfbB"
                     /locus_tag="LP859_001693"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL3029653.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10897..11748
                     /gene="rfbD"
                     /locus_tag="LP859_001694"
     CDS             10897..11748
                     /gene="rfbD"
                     /locus_tag="LP859_001694"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL3029654.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11782..13815
                     /locus_tag="LP859_001695"
     CDS             11782..13815
                     /locus_tag="LP859_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029655.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13859..15138
                     /locus_tag="LP859_001696"
                     /pseudo
     CDS             13859..15138
                     /locus_tag="LP859_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15348..16154
                     /locus_tag="LP859_001697"
     CDS             15348..16154
                     /locus_tag="LP859_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029656.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16166..17386
                     /locus_tag="LP859_001698"
     CDS             16166..17386
                     /locus_tag="LP859_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029657.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17376..18962
                     /locus_tag="LP859_001699"
     CDS             17376..18962
                     /locus_tag="LP859_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3029658.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19001..21139
                     /locus_tag="LP859_001700"
     CDS             19001..21139
                     /locus_tag="LP859_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3029659.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21507..22490)
                     /locus_tag="LP859_001701"
     CDS             complement(21507..22490)
                     /locus_tag="LP859_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL3029660.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7171 a         3899 c         4712 g         7070 t
ORIGIN      
        1 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
       61 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
      121 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
      181 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
      241 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
      301 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
      361 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
      421 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
      481 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
      541 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
      601 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
      661 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
      721 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
      781 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
      841 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
      901 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
      961 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
     1021 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
     1081 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
     1141 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
     1201 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
     1261 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
     1321 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
     1381 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
     1441 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
     1501 taattttgca tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat
     1561 aaaaaaatta aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat
     1621 tttggcagct gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa
     1681 catcgcgaac aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga
     1741 tgacttgaga caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac
     1801 tgttgagcat acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag
     1861 aagagagaag aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat
     1921 gcagaatttc tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa
     1981 tctttcagaa gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat
     2041 cgcagctttg atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat
     2101 ccaagacagt atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata
     2161 ctcaaaagtt ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt
     2221 ttttcttaca gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata
     2281 aaagagccat acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt
     2341 ttgcgactag ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca
     2401 gatccacttt aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga
     2461 aatcatccac cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt
     2521 aaatttttcc caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag
     2581 gcagatcgcc atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt
     2641 catctgcatc tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg
     2701 cttctgcaac aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt
     2761 ttgctaaagc catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca
     2821 aaacatcatt gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat
     2881 gtatgccgct atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt
     2941 taaaatcagc acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac
     3001 ataaaaatct gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg
     3061 tcagaaacta tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg
     3121 aatatgcgac ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc
     3181 ctttgaaaaa cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt
     3241 ctgattcttg ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg
     3301 aagtaacgaa gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg
     3361 gtgacatcgg tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg
     3421 attttattgg tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg
     3481 gagaagctgg aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg
     3541 ttaataccgg aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag
     3601 acggcggact ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa
     3661 ttttgacatc acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag
     3721 aggtacatga aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag
     3781 acgactcaaa agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat
     3841 tatttactct tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc
     3901 cacatcgatc aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac
     3961 cagtttatcg gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc
     4021 gtttctgcat tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg
     4081 atgaataaag cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca
     4141 ctaattgtca ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg
     4201 ggacagctga ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat
     4261 cagttccaaa ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg
     4321 atttatttaa ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc
     4381 ttcgctactg tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt
     4441 gcaacgagga tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt
     4501 aattttgctg ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac
     4561 gtgacgggtc atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg
     4621 aaaactaaat tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc
     4681 agtaatttat aataattatg actcttaatt aaagtaggtg cccatatgct tgtttctttt
     4741 agtatggtta taagattttt tctgacaatt ttattatcac tgatattaac acctgtattc
     4801 aaaatcattt cagtacaaac aggtatggtc gacaaaccaa atgaaagaag aataaataaa
     4861 gttccaatgc cttcagcggg cggactgcct atctttattt catttgttat atcgacttta
     4921 ttcttattcc gtaaaattat ccctcaatcg tatattgtac cgatcttgat tgcttcttta
     4981 gtaatcatag tcacaggagt gctcgatgat aaatatgagc tgacacctaa acaaaaaagt
     5041 atagggatct tgattgcttc tcttattatt tattttgtag cagatatacg tattgattcc
     5101 ttcacgcttc cttttattgg atatatccaa ctaggctggc ttagttttcc cgtaacgatt
     5161 ttttggatct tcggtatcac gaatgcggtc aatctgattg acggtttgga tgggctggcg
     5221 gcagggattt cattgatcgg tttaattacg ataggaatca ttggatattt tttcctccat
     5281 gcttctacag tgtatattcc agttgttatt ttctgtcttg tagcaagtat tatcggtttt
     5341 tttccttaca atttttatcc ggcaaagatt tatttaggcg atacaggagc tctattccta
     5401 gggtttatga tggccgtcct ttcactacaa gggctgaaga acgtgacttt tgtttcatcg
     5461 atttctttgc tgatcgtcat gggagtgccg gtaacagaca cgttttttgc tatcattagg
     5521 agaaaagcaa accgtgtttc tttttctaca gcagataaaa aacatcttca tcaccgacta
     5581 atcagtttag gctttactca taaaggtgct gtcctgacga tttatgcgat ggctttgatg
     5641 ttttctttca cagctatggt catgaattat actggaagaa tcggaacgat catattgatt
     5701 attgccatgt tgtttgctgc aattttatta tttgaattga ttggtctgat caatgagaaa
     5761 catcagttta ttttaaaaac attgcgattt ttagggaata aagagtaccg tacgcaagta
     5821 aaacagaaat atttgaagaa atggaaccgt catatagatg aataacaaat taaaccagcc
     5881 gatccttatt agtattgtga cggcaaacag tgataaaatt tttcggacac tagataattt
     5941 tatttcaact gtaaaagatg aaacagcagt aaaaatccga atttttgaca acaattcact
     6001 tcccgagtac aaaaaaagac taacagagta taggaaatat ccctacatcg atatctattt
     6061 tcatgatgaa aattccggat ttggttatgg acataatcac aatctgatag ctagcgattc
     6121 tcgctatggc attatttgta acccagatat tttagtggat gaagagacag ttacagcatt
     6181 ggtcttgtta ttgaaagaac atccggaatg tgcgatgtta gcacctaaga ttcttaatga
     6241 agatggaaca actcagcacc tcatccgtga gcagctggct gtttttgatt acgttttgcg
     6301 ctttgtgcct ttcaaatggg tcaaaaaaaa gtttgacaaa cgactggctg tgtttgaatg
     6361 ccgaggattg cctactgatg ctaatagtta tgtacggatg atctcaggta gttttatggt
     6421 agctgatatt gataaattta aagagataaa ggggtttgat gagcgtttct tcatgtactt
     6481 tgaagacaac gacttgtgtt tgagttttga aaaagcaggt tacaagttat tgtacacacc
     6541 cttttatgca gttatccatt tttacgggaa gggtgcgcat cgaaatgcaa aactgtttcg
     6601 cgtgtttttg acttctatga tgaaattttt caataagtgg gggtggcgtt ttttctgatg
     6661 aaaccagaaa tattagtagt cgttgtgctt tatcagcaac tattcagtca atctccagct
     6721 tatggaccat taagcaaagc acttagcgaa aagcaaattc agcttattat ttacgataat
     6781 agtcctcaaa ggcagcaaaa tgagctgttt gaaaaagaaa atatcgttta tcatcatgat
     6841 ccgcaaaatc ctggtttagc ggtggcttat aattttgcgt tgtctcaagc agatgaagga
     6901 acacgttgtc tcgtcactct ggatcaggat acgaggctag tcgacgatta ttttgaacta
     6961 ttgcgtaaag taacctttac agatgaatgt gttgctgcag ttccgatgat tttttctggg
     7021 agccgacaag tttctcccct atattcagat cgttatatca atcgtacagc tcaagcagta
     7081 gaagttgaag gcccaacagc tgaacgaatc atggcaatca attcaggtgt tgcctggtct
     7141 gtcaaatttc taaaaaagat tggcgggttt aatcctgctt tttctttaga ttttttggat
     7201 cattggctgt tttggaaagt ggatcagttg gaaaaaactg tggagatcct tcctgtacga
     7261 atggaacatg atctttcggt tttagactat gaaaaagttt caagtaatcg ataccgttct
     7321 attttacaag cagaaaatcg attttatcat gaatatgatc aaaggcattt atccagtcac
     7381 cgacgacaat tattgttaag aacagctaaa cagttcttga ccgtcaaaaa ccggcatatc
     7441 tggcggatga ctttgcgctc atatgttaag aattggaagg tgtgaattcg tgcgttcggt
     7501 ttgtatagct acctttaatg gaggcagata tttaagagag cagttagata gtattttatc
     7561 tcagctttca gcagaagatg aagtagtcat ttctgatgat gggtcagtag atgatacatg
     7621 ggatatatta actgaatacg cagaaaaaga taagcgaatc cagctttttt cggggcctcg
     7681 tcaaggattg atcgctaatt ttggttatgc gatccagcag acgaaaggag atctaatctt
     7741 tttggcagac caagatgatg tttggttgga gacaaaagtg aaaaagatca ctgactattt
     7801 tgagcagaat ccggatcagc tagtagtagt ttctgattta atcattgtgg acagcgagct
     7861 gcaagagatc catgattcgt atttttcgtt tcgaaattcc aaagaaggat ggcttaataa
     7921 tgtgataaga agtcactata ttggtgctgg tatggctttc cggtcttctt taaaacccga
     7981 tattttgccg atcccgtcag aagtgccgat gcatgatatg tggattggtt tgctgggagg
     8041 aaagaagact gggttcctaa gagaaccgtt aactctgtac cgaagacatg atttaaacgc
     8101 gagtgagatt gaaacacatt ctaaattacg gcaaaaaatt tcttggcgtg ttcatctgat
     8161 tacggcgttg atcaaacgaa aatggtttaa acgttgataa aagatgttta aatccttaat
     8221 atttccatga caaatataac aattgatgag ataataacaa agtgaaattt gaaatcaaaa
     8281 aggagagcag atatccatga agggaatcat tcttgctggg ggcagcggaa cacgtctgta
     8341 ccctttaaca aaggcaacat cgaaacaatt gatgccgatt tatgacaaac caatgattta
     8401 ttatccaatg tctactctga tgttggcggg aatcaaagaa attctgatta tttcaacgcc
     8461 acaagataca ccacgtttca aggaattatt tggaaacgga aatgatttag gactgcagat
     8521 cgaatatgca gtacaagaaa gtccagatgg attggcacaa gcgtttatca ttggagaaga
     8581 atttattgga gatgatagtg tctgcttggt attaggggac aatatttact atggtggcgg
     8641 tctgtctaaa atgttgcaac gagcagcttc aaaagataca ggcgcgacag tatttggcta
     8701 tcatgtcaat gatccagaac gctttggtgt agtagaattt gatgagaata tgcaagcttt
     8761 gtcgattgaa gaaaagcctg ctcagccaaa atcaaattac gctgtaactg gcctttattt
     8821 ttatgacaat gaggtaattt ctatcgctaa aggtatcaaa ccttcagaac gtggagaact
     8881 agagattacg gatgtcaata aagcatattt ggataaagga aaactatccg tagaacttat
     8941 gggacgcgga tttgcttggc ttgatacagg aacacatgaa tcattgcttg aagcatctac
     9001 ctttatcgaa acaattgaaa aacgtcagaa cttaaaagtg gcttgtttgg aagaaattgc
     9061 ttaccgtatg ggctacatcg ataaggaaca attgctggcc cttgcacagc cattgaaaaa
     9121 aaatcaatac ggtcaatatt tattgaattt ggccgctgaa taaggagtcc ttacagatga
     9181 aagtaacaga tacaaaatta acagatgtca aaatcgtaga aatggacgtc tttggcgatc
     9241 atcgcggttt cttcacagaa agttattcaa aagcaaaatt tgcagaacat ggattgaaat
     9301 atgactttgt tcaagacaac cattcactat cagctgaagc tggtgtcctt cgggggcttc
     9361 atttccaaaa aggagaagct gcacagacaa aattgattcg ggtagtcact ggagcagttt
     9421 tagatgtagt agtggatatc cgcaaaggta gcccgactta tggtcaatgg gacggattta
     9481 tcttatctga acataatcat cgacagttgc ttgtacctaa aggatatgcc catggattcg
     9541 ttactttgac accaaatgtg aacttccttt ataagtgtga taattactat aatgcagaag
     9601 cagatggggg catcgcattt gatgatccag atttagcgat tgattggcca attgatccat
     9661 caagagcgat tatgtcggaa aaagatcacc gtcatccgac attaaaagaa tttgaagcag
     9721 aaaatccatt tgtttacgga gaaatctaag aattaaggag aaattttctt atgaaaaaca
     9781 tcattgttac cggaggtgcc ggattcatcg gctctaattt cgttcattac gtggtaaaca
     9841 atcatccaga agtgcatgtc actgttttgg ataaactgac ttatgcagga aacaaagaga
     9901 acttggctgg acttcctagc gatcgcgtag aattggtcgt aggagatgtt gcagatgcag
     9961 aattagtgga ccgtctagta aaagaagcgg acgcagttgt tcattacgcg gcagaatccc
    10021 ataacgataa ttcattaaaa gatcctttcc catttgtaca aacgaatatc attggtacgt
    10081 atacactttt agaagcttgc cgcaagtatg atgtacgcta tcatcacgta tctactgacg
    10141 aggtttacgg cgatcttcca ttacgtgaag acctgccagg acatggggaa ggcgaaggag
    10201 aaaaattcac tgcggaaaca ccatataatc cttcaagtcc atattcttct acaaaagctg
    10261 gttcagatct gttagtaaaa gcatgggtgc gttcttttgg cttacgtgca accatttcaa
    10321 actgttcaaa caactatggt ccttatcagc atattgaaaa atttattcct cgccaagtaa
    10381 cgaatatctt aagcggtatt cgtccaaaat tatatggcga aggaaagaat gttcgtgact
    10441 ggatccatac gaatgatcat tcttcagcag tgtggctgat tttgacgaaa ggcagaattg
    10501 gcgaaaccta tctgatcggt gcagacggtg aagaagataa taagaccgtg atggaattga
    10561 tcttagaaat gatgggtcaa ccaaaagatg cgtacgacca tgtaaacgac cgtgcaggac
    10621 acgacttgcg ttatgcaatc gattcgacaa aattacggga agagttagga tggaagcctg
    10681 agtttacgaa cttccgtgat gggttggctg acacgatcaa atggtacgaa gaacatgaag
    10741 actggtggaa aaaagaaaaa gaggccgttg aagcagcata tgccaaaaat ggacaataat
    10801 acagagaaaa tgtcactgta ttaatggaaa aacacatgtg aggcaggcgc tcgcctgtct
    10861 cttttttaaa ataaaaatca aggagcggat aatcatatgt ttttattaac tggaggaaat
    10921 gggcaactag gtacagaatt gcgccattta ttagatgaaa aaaatctgca atatgtttca
    10981 acagatgcaa aagacttgga tatcacagat gccgagaaaa caatggctta catcacagaa
    11041 ttaaaacctg atgttatttt ccattgtgca gcttacacag cagtagataa agcagaagaa
    11101 gaagcaaaag aattagatga gaagatcaat gttgatggta caagaaatgt agcacttgca
    11161 gcaaaagctg tgaatgctca attcgtctat atcagtacag actatgtttt cgatggaaaa
    11221 aacaaaaatg tagaatacga agtagacgac caaacgaatc cactaaatga atatggtcgt
    11281 acgaaattat taggcgaaaa agcagtagaa gagattttag aagactatta tatcatacga
    11341 acttcttggg ttttcgggat ctatggacac aatttcgtct tcacgatgca acgcctagcg
    11401 gaaacccgcg acaaactgac tgtagtaaat gaccagtttg ggcgaccaac ttggacacgt
    11461 actttggcag aatttatggt atatgtcatc gaagaaaaag caccatttgg tatttatcat
    11521 ttgtcgaacg aaaatagctg ttcttggtat gaattcgcaa aagaaatatt gaaagataca
    11581 gatgtagaag ttgcacctgt tacgtcagaa gaatttccgc aaaaagcaac acgtccgcag
    11641 tattcagtca tgagcctgaa aaagacagaa gagttaggat ttgtcatacc aacttggcaa
    11701 gaagcacttg cgcaaatgct ggaagctgcc aaaaaaatga ataagtaatt tccttttatc
    11761 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
    11821 ccaaaagcga accaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat
    11881 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
    11941 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
    12001 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
    12061 caagctgaag aggactttcc gttggtgaac gaggattatt cattaaacgg acaaaacatg
    12121 atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg
    12181 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
    12241 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagagaaa aaaagctcta
    12301 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
    12361 catgtgggcg atttgatttt ctttactttg ggactgatga ctgcttttta tcattatttc
    12421 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
    12481 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
    12541 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
    12601 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
    12661 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
    12721 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
    12781 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
    12841 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
    12901 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
    12961 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
    13021 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
    13081 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
    13141 tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt
    13201 gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac
    13261 ctcgcttttg ccgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
    13321 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
    13381 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
    13441 aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg
    13501 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
    13561 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
    13621 gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac
    13681 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
    13741 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
    13801 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atgttagtag aaaggagagt
    13861 ggagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
    13921 tttgttactg acgctagtct tttttgtttg gacagcaggg ttaggttttc cagcaacttt
    13981 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
    14041 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
    14101 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt
    14161 tcctttgatt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
    14221 cggtatttat ctgattgtct tttcggtctt cctcggaatc acgtttatcg tgtatttaca
    14281 gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct ttctgatatt
    14341 tggatcaata cgaatacaat cggaagttct ttgatgactc ttgctttatt gatcagtgga
    14401 tttgccagtt cttttgaacg atggtatgta cgaatcatcg gtgcagtagc agttgttgca
    14461 gcttttctcg ggatttgggt ctgccaatct agaggtgcat tagtcgcgtt gattgttttt
    14521 gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc atttattgct
    14581 tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt ctctgaaaaa
    14641 gtcaatttgt ttaccggtcg agaagatatt tggcataaat tctatcagac gctaggagaa
    14701 aaatctgaac aaattttact aggaatgaaa acatttatct tccaacgagg aaaccaattt
    14761 ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat aggatttggg
    14821 attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc agatttatcc
    14881 aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc atttatggag
    14941 gatacattga cttcataacc atgggttccg attatttata tcctgttggc tatggccgtt
    15001 catcgatatg atgaaccaga agttgcggag gaattctatg ttatggaaga ggcatatgac
    15061 gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca ttcttccagg
    15121 atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac cgtcgcagcc
    15181 ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc
    15241 ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa aatatggtaa
    15301 aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg tttaaagatt
    15361 tgtatttatt tatgaaggac atttatcaga acagaaaatt attgctacag ttttcgatca
    15421 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
    15481 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
    15541 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
    15601 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
    15661 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
    15721 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
    15781 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
    15841 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
    15901 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
    15961 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
    16021 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
    16081 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
    16141 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
    16201 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
    16261 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
    16321 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
    16381 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
    16441 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
    16501 aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
    16561 tgaatttgct gacatcggag accatattta tcaacctgta aaaacttatt caagcgggat
    16621 gttcgttcgt ctagcttttg ctgtagcaat caatgtgaat cccgatattt tgattgtgga
    16681 tgaagcattg gcagtaggcg atcttgaatt tcaactaaaa tgtatggaaa aatttacaga
    16741 aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatatcagtt cgattcgtcg
    16801 tttttgtgat cgttcctatt ggttgaaaaa cggagaagtc gtcgaatatg gcgatacgat
    16861 ggaagtaacg acgaattatg aaaacttttt aaagaaaaaa tcagttaaaa cagtggatcg
    16921 gaataaaaac caagtagaac ggttcgccgc tccagatatc gtcgacgtga cgaaagcgga
    16981 attgttggat aaagacaaaa atccggtgga tatgctggaa caaggagaaa aactatacgt
    17041 taaagtaact tatgaagtaa aagatgatac aatcaaaaat ccggttttag gaattgcgct
    17101 acgaacagtc gacaattttt atgtatgcgg acttaatact ctactagatg gtatcaagat
    17161 tccttggaaa aaaggagaga atatctttta tctggagtat gaaaagttag gtttgttagc
    17221 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
    17281 taagacaaag tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
    17341 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
    17401 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
    17461 tagagtttgg tcccggaaac ggccgattaa cgaaacattt aatcggagca aaacaatgtg
    17521 aagtgagcat tgttgagtta gataaggaat tgttcgattt tgtcagcgaa tttgctcaag
    17581 atggatttta tggtgatatc gaaagttttg agtgggcaaa ctattatgct ggacagacat
    17641 ttgattacgt cttgtttgcg gatgttttag aacatttggt agatcctgga aaaacattga
    17701 agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc cctaacttgg
    17761 cgcataattc tgtcatgatc gatttgttca ataaccaatt gccgtgggct tcttatggtc
    17821 tcctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa gtttttgaaa
    17881 aagcgggatt attcattaat attgaagatt atttgtactt agctgtaggt gatacagaat
    17941 taaaatcaac ttatgaagaa ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat
    18001 ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagca caaagttcca
    18061 ttgcagagcc acaaaattct aattatgtaa aagtgttaga agtaacccag caaacgaaac
    18121 aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa
    18181 gtttccccgt atcagaaaca acagaaagaa tggtgtttcg cttcgcgcaa caacctagct
    18241 ttatcgaatt cagcgcagaa gctgccggag aaaaactaac atttatcgat tcgaatgctg
    18301 tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaattacca gagttcgttc
    18361 taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat
    18421 tgacaccaac aatgaaagaa ttgttggaaa cgattcgtcc catggctgaa gtcacaaagc
    18481 agctttctga gaaaaatgac gaattagaaa gagaaaatca tcatcttctt gaggaaaata
    18541 caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg
    18601 atgaatggac aataaaacat agactaggac gacgtaagaa agaaacttcg aaaaaaatcc
    18661 aagaaaaaga actttctata tgtattgacg agaaaatctg ggatgcagaa acaaaaatat
    18721 taaaaatcat cggatgggga atcgctaatt cggatcgcca gcccttgtcc tataagttat
    18781 cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagaagaa gtcaatcaag
    18841 cagaacagct cgcaaaagga acagaagcgg gatttgagtt acgaatcctt tgcgagcaag
    18901 aacgttcatt cttagtggaa gcagtagctc aaaatggcca atcatggatc attgaaatgt
    18961 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
    19021 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
    19081 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
    19141 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
    19201 gacccaaagc ggttttaagg tacgtttatc agggaaaatg agaggaaaag ccatcttaga
    19261 cttccaaaca gcgagacatc aaaatggcat agctgtgaaa ttaaacggta aataccctta
    19321 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
    19381 ttatgcaagg actcatggcg taaaaaaagc gatccgtcgg gtcaaattgg aattgaagcc
    19441 aggatcgatc gactatgcag agtggatcag tcgtcatgag aaaccagatt tgaaagagca
    19501 aaggaaagaa gcacaaggat ttgcgtaccg tccattgatt tccatactta tgcctgtcta
    19561 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtactggatc aaacgtatga
    19621 tcattgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
    19681 ggaatcctat caagcaaaag atgatagaat caaagtcgtt ttccgaaaag aaaatggaca
    19741 catcagtctg gcaactaatt ctgcattgga aatagcagaa ggagaattta tagcactctt
    19801 agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacgt
    19861 acatccagaa ctggatgtga tctacagtga cgaagataaa atcgatgcag atggcaatcg
    19921 ctttgatccg catttcaaag cagactggtc gcctgataca ttgatgggaa ataattatat
    19981 ctctcactta ggcgtatatc gaacaagcat tgttaaagaa cttggcggtt tccgtaaagg
    20041 ctatgaaggt tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga
    20101 tcatatttac cacattgacc gagtcttgta tcattggcgg acgatcccag gatcaactgc
    20161 aagtaatggc gaagccaaaa gttatatcta tgattccgga gtgaaagcat tgacagatgc
    20221 cctgtctaga agaaacatca aaggaagcgt acgtcctggc agaatttctg gattttatga
    20281 gattgcttat gacgtcttgc aagaggattt ggtcagtgtg atcattccaa cgaagaatgg
    20341 ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt attcaaatta
    20401 tgaaatcatt attgcggata acggaagtac agatccaaaa atgcaagaat tatttgcaga
    20461 atacaagcat cagctgaaag accgctttat cgtagaattg attgacattc cattcaatta
    20521 ttctcgaatt aacaatttag cagcagaaaa agcaaacggc aaatactttt tattcttgaa
    20581 taacgataca gaagtgattg agccagattg gatgacggtg atggtttctt atgcacagtt
    20641 tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc
    20701 aggagtatta ttaggaatcg gtggtgttgc agggcatgca ttgaataact atgatcgaac
    20761 acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
    20821 ttgtatgatg gtcaaggcag ctgacttcaa tgctgttaac gggtttgatg aaacattaga
    20881 agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc gttacaatgt
    20941 ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt atgaagatac
    21001 tccagaaaaa caaagacgtt ttgctggtga aatcaaaaaa atgcaagata aatggccagc
    21061 atatattgcg catgatccat tttacaatga taatttaaca aaagaaggaa tcggcgactt
    21121 ttcgttgaga ccagattaaa ctataataaa aagctgttat gttgtgattc gctcgcacat
    21181 aacggctttt tatagttttt tgtcaaaaaa aggcagaagg tgccgactca aaaaaataag
    21241 tcgaggttgg gacagaagtg tttaactccg agaaataaga agaaattcac gaaaattgct
    21301 tttcaaattt ttgtgaattt cggcttattt aagaagaagt tgcttctgct cccaccgttt
    21361 attcgttttt aaagaatgag acaaaagtgt ttcttacttt tgtcccacgc tcttattcat
    21421 gctcagagcg ccaatagata caaaatcata gccaaagttt cacgaaccct agctgtcaaa
    21481 gaatcgatat tttattttaa tataggctat tttaaaataa atggtttaag gtattcctct
    21541 acaaaagcca ctctatcaat tatcatgggt ttaaaataag aagtgatgga gataaccaga
    21601 ttgttcttag cgtcaatata aagaacattt ccactattac caattgctga atatatttcg
    21661 ttctcagaat ctaataccca ccataaatag ccatatctca tattttgtaa gtcttctaaa
    21721 gcactggttc taggtttgaa catctcgttt atccacatgg gtggtacgat ttgttttcct
    21781 ttatacatcc ctttatttag gcataataca ccaatttttg ccagatcact tgccgataaa
    21841 cacaaaccat atcctgccgt cccgatattt agtggatcac aaaaccagac attggtttta
    21901 ggagacttat ccattgtaaa ctgcttatgt tcttcagctg tttcagcata ataattgaca
    21961 tggtttggta tccctatagg atcaaataga tacttattgg caaaatcaac agttgttagc
    22021 ttacttgtct gatacaacaa gccagttaat atgtgtaaac agacagtttg atagttgaat
    22081 tcgccggtta taccttttct tcctcccaat aaatctagtg atgtttttgt ccaatcttca
    22141 cttgaacaga cttttgtcca tggatcgcct ttacatttgt agggtgcttt cattgttagc
    22201 aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt atactctgga
    22261 aaataatcta atactttatc atcgatactt ttgattaatc cttgctctaa agcaatgcca
    22321 atcagcaaag atacaatact tttcgttgct gacatgatat gacaagtatc gtctttttta
    22381 taaccattcc attcatctgt ataaacttct accccatcct tatatgcgac aatttgacat
    22441 atatttggtt gtcgaaaacc aataaaatca tgcaactctt gtttattcat aagatattac
    22501 ctcactttct tctataagca taatgtcatt aaaaatcgat ttcaattttt tcgattcttc
    22561 acgtaataca ggtattctta tggactagac agcgctttgg gaagatggat tctctgaaaa
    22621 atataaaggt tcactttatg agtggataaa aaaagcagca aatacttcga cttctttaat
    22681 ttaccaacat tagtgaaaat cgattcatct ggagatttgt aatttagagt ccttgtcttg
    22741 attagaggtg tataaagctt tttattagac atttttaaat tccattacaa tttgcttata
    22801 aactaggttt ttaaaggtga ctatggtaag ataatagaga tattattttg tg
//
LOCUS       DAFEZB010000043        22740 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFEZB010000043
VERSION     DAFEZB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.043"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            103..273
                     /locus_tag="LP859_001702"
     CDS             103..273
                     /locus_tag="LP859_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029661.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            493..1200
                     /locus_tag="LP859_001703"
     CDS             493..1200
                     /locus_tag="LP859_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3029662.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="LP859_001704"
     CDS             complement(1259..1903)
                     /locus_tag="LP859_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL3029663.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="LP859_001705"
     CDS             2030..2317
                     /locus_tag="LP859_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029664.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="LP859_001706"
     CDS             2425..2745
                     /locus_tag="LP859_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029665.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="LP859_001707"
     CDS             2826..4139
                     /locus_tag="LP859_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL3029666.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="LP859_001708"
     CDS             4136..4855
                     /locus_tag="LP859_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL3029667.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="LP859_001709"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="LP859_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL3029668.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="LP859_001710"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="LP859_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL3029669.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="LP859_001711"
     CDS             join(8416..8487,8489..9517)
                     /gene="prfB"
                     /locus_tag="LP859_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL3029670.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="LP859_001712"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="LP859_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL3029671.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="LP859_001713"
     CDS             10292..11185
                     /locus_tag="LP859_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029672.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="LP859_001714"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="LP859_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL3029673.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="LP859_001715"
     CDS             13122..13979
                     /locus_tag="LP859_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL3029674.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="LP859_001716"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="LP859_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL3029675.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="LP859_001717"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="LP859_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL3029676.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="LP859_001718"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="LP859_001718"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL3029677.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="LP859_001719"
     CDS             16735..17493
                     /locus_tag="LP859_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL3029678.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="LP859_001720"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="LP859_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL3029679.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="LP859_001721"
     CDS             18406..19437
                     /locus_tag="LP859_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL3029680.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="LP859_001722"
     CDS             19434..20201
                     /locus_tag="LP859_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL3029681.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="LP859_001723"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="LP859_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL3029682.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22045..22353
                     /locus_tag="LP859_001724"
     CDS             22045..22353
                     /locus_tag="LP859_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL3029683.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            22353..22703
                     /locus_tag="LP859_001725"
     CDS             22353..22703
                     /locus_tag="LP859_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL3029684.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7462 a         3922 c         4908 g         6448 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatactgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
//
LOCUS       DAFEZB010000044        21922 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFEZB010000044
VERSION     DAFEZB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.044"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            282..521
                     /locus_tag="LP859_001726"
     CDS             282..521
                     /locus_tag="LP859_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029685.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="LP859_001727"
     CDS             complement(883..1143)
                     /locus_tag="LP859_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029686.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="LP859_001728"
     CDS             complement(1328..1825)
                     /locus_tag="LP859_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL3029687.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="LP859_001729"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="LP859_001729"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL3029688.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="LP859_001730"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="LP859_001730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL3029689.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="LP859_001731"
     CDS             complement(4547..5209)
                     /locus_tag="LP859_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL3029690.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="LP859_001732"
     CDS             complement(5219..6034)
                     /locus_tag="LP859_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3029691.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="LP859_001733"
     CDS             complement(6296..6739)
                     /locus_tag="LP859_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL3029692.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="LP859_001734"
     CDS             complement(6873..7211)
                     /locus_tag="LP859_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3029693.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="LP859_001735"
     CDS             complement(7199..7576)
                     /locus_tag="LP859_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3029694.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="LP859_001736"
     CDS             7800..8993
                     /locus_tag="LP859_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3029695.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="LP859_001737"
     CDS             complement(9159..9581)
                     /locus_tag="LP859_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029696.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="LP859_001738"
     CDS             9976..10170
                     /locus_tag="LP859_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029697.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="LP859_001739"
     CDS             10167..10661
                     /locus_tag="LP859_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029698.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="LP859_001740"
     CDS             complement(10823..11995)
                     /locus_tag="LP859_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3029699.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="LP859_001741"
     CDS             complement(12201..13016)
                     /locus_tag="LP859_001741"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3029700.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="LP859_001742"
     CDS             complement(13166..13612)
                     /locus_tag="LP859_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL3029701.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="LP859_001743"
     CDS             complement(13609..14613)
                     /locus_tag="LP859_001743"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3029702.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="LP859_001744"
     CDS             complement(14653..14982)
                     /locus_tag="LP859_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL3029703.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="LP859_001745"
     CDS             complement(15108..17435)
                     /locus_tag="LP859_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3029704.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18278..18571)
                     /locus_tag="LP859_001746"
     CDS             complement(18278..18571)
                     /locus_tag="LP859_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL3029705.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18974..20151)
                     /locus_tag="LP859_001747"
                     /pseudo
     CDS             complement(18974..20151)
                     /locus_tag="LP859_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20255..20569)
                     /locus_tag="LP859_001748"
     CDS             complement(20255..20569)
                     /locus_tag="LP859_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029706.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20676..20891
                     /locus_tag="LP859_001749"
     CDS             20676..20891
                     /locus_tag="LP859_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029707.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21191..21433)
                     /locus_tag="LP859_001750"
     CDS             complement(21191..21433)
                     /locus_tag="LP859_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029708.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21420..21848)
                     /locus_tag="LP859_001751"
     CDS             complement(21420..21848)
                     /locus_tag="LP859_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3029709.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6454 a         4313 c         3706 g         7449 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
    17521 tttggggctt tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa
    17581 gattttattt ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa
    17641 cttgattaat tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag
    17701 gacgggatta tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga
    17761 gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caactagctg
    17821 atcttgtatt cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc
    17881 taacgctcag gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt
    17941 ttcaagaacg aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga
    18001 ggtacgtttt ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca
    18061 tccaattgat tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac
    18121 tgcgaatcgc tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt
    18181 tgtattatag atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca
    18241 tacttgaact tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct
    18301 gatatgcccg accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt
    18361 gttcgacata atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg
    18421 tataagtcac tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt
    18481 ttgttgaact tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca
    18541 tcttttcttg ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac
    18601 tccatttatg ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat
    18661 tatcatagtg ctcttttttt tactactact tataggtaga tttattctaa tccattattc
    18721 tcccctacca ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa
    18781 aaagaaccct tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat
    18841 ttctttgata cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct
    18901 gactacatat tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc
    18961 tttcaccgtt tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat
    19021 ttgacgttaa gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt
    19081 tcaagacttc ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat
    19141 gagtatgaaa tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta
    19201 catactctcg acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg
    19261 tttcttctat aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt
    19321 gtagcttttc cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag
    19381 tagtttcaat tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa
    19441 tttcatttct tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc
    19501 agccagtgta aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc
    19561 cataattcaa aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta
    19621 tggcttcttt taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg
    19681 gattttcact tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa
    19741 taagtgattg taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct
    19801 ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac
    19861 caatccatcg aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt
    19921 gttcatttaa cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat
    19981 tgcttgcaac aattttttct taagcctttg ttgtttctcc atttccagat aagcgagttg
    20041 cgcctcacgt tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt
    20101 gatcctacca gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt
    20161 cctcctattt tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc
    20221 atcattgtac cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt
    20281 tttctcgata gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat
    20341 agtgtcctcg aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca
    20401 gtgtactatc acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta
    20461 aatgactgac tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat
    20521 agaactctat ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct
    20581 ctcaaaatca aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact
    20641 ccatcaaaac ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa
    20701 aaataattaa agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa
    20761 tcaacttgag tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc
    20821 ttgccgatgc tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa
    20881 tatggaaata gtagtttttc tgatctatca gctgttttgc caaagcacat aggaatctca
    20941 cctcccccca gtaatggcgg gccgatttgg ttacggaagt atcattatcg tagtcgcgat
    21001 tctcatatta aaatttgtcg cttaaaagct aaccttgtca tggcgtaact agagaatcaa
    21061 aatctacatg tactgataga tttgctattc atttgtcaaa gagcgctagg ctattttcag
    21121 cctatggaaa tcctttattt tgacccgata aaaaacttcc atagaatgaa aaggaaaggg
    21181 aaaaaatact ttaacgagtt gtatcaaatt caagtatctt ctcaatcaat tttgtttcca
    21241 gttgtctacg catatattca tctacataca taaccattcc tttttcttct tcataaactg
    21301 gtttcatggc taaccgtgaa atataatttt gatattttga cagaacgatg gacatggcaa
    21361 ttgaatcacc agaagctgcg gctttgattg tcgttagtgg tatgaatggt atatatcgtt
    21421 tattttgctt catatttgac acccaaatac tttcctagct cttccaaagc catttgtcgt
    21481 tggtaccaaa cactgccgag acttttccca ataatatcgc caatttcacg atcattaaga
    21541 ccgatgaaga aggacataaa aatgatttct tgtctttgac taggtaatgc tgcaatcgca
    21601 tttgccaact cctcattgga aataaagatt ttcataccaa ggacaataaa agtaccacta
    21661 ctattcaaat ttggttccaa ataatagaac tctgtaccta accctttttg ttccaatatt
    21721 tccattgata tttgccgttg gcgaaatcta gcgtacttct tttgaatatt taacgcctca
    21781 ttgcagacta ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt ttcctctgag
    21841 tcgcccataa tattctcccc ctcaaaaaat tcattcaact cgttttactt ctatcccttt
    21901 ccattattac tacaatctag ga
//
LOCUS       DAFEZB010000045        21505 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFEZB010000045
VERSION     DAFEZB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.045"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            5..403
                     /locus_tag="LP859_001752"
     CDS             5..403
                     /locus_tag="LP859_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL3029710.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="LP859_001753"
     CDS             387..1082
                     /locus_tag="LP859_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL3029711.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="LP859_001754"
     CDS             complement(1666..2553)
                     /locus_tag="LP859_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL3029712.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="LP859_001755"
     CDS             2738..3577
                     /locus_tag="LP859_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL3029713.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="LP859_001756"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="LP859_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL3029714.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="LP859_001757"
     CDS             5045..5401
                     /locus_tag="LP859_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029715.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="LP859_001758"
     CDS             5404..6561
                     /locus_tag="LP859_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029716.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="LP859_001759"
     CDS             complement(6587..7027)
                     /locus_tag="LP859_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL3029717.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="LP859_001760"
     CDS             7187..7693
                     /locus_tag="LP859_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3029718.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="LP859_001761"
     CDS             7861..9774
                     /locus_tag="LP859_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL3029719.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="LP859_001762"
     CDS             9779..10780
                     /locus_tag="LP859_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3029720.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="LP859_001763"
     CDS             complement(10982..11188)
                     /locus_tag="LP859_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL3029721.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="LP859_001764"
     CDS             complement(11834..12682)
                     /locus_tag="LP859_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3029722.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12681..13748
                     /gene="gnd"
                     /locus_tag="LP859_001765"
     CDS             12681..13748
                     /gene="gnd"
                     /locus_tag="LP859_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL3029723.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="LP859_001766"
     CDS             13750..14751
                     /locus_tag="LP859_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL3029724.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="LP859_001767"
     CDS             14748..15032
                     /locus_tag="LP859_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL3029725.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="LP859_001768"
     CDS             15065..15862
                     /locus_tag="LP859_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3029726.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="LP859_001769"
     CDS             15876..16709
                     /locus_tag="LP859_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3029727.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="LP859_001770"
     CDS             16727..17584
                     /locus_tag="LP859_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3029728.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="LP859_001771"
     CDS             17606..18115
                     /locus_tag="LP859_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029729.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="LP859_001772"
     CDS             18152..18553
                     /locus_tag="LP859_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029730.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="LP859_001773"
     CDS             18665..19018
                     /locus_tag="LP859_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029731.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="LP859_001774"
     CDS             19052..19495
                     /locus_tag="LP859_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL3029732.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="LP859_001775"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="LP859_001775"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL3029733.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="LP859_001776"
     CDS             20590..21303
                     /locus_tag="LP859_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3029734.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7401 a         3302 c         4491 g         6311 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttcca
//
LOCUS       DAFEZB010000046        20664 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFEZB010000046
VERSION     DAFEZB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.046"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            168..362
                     /locus_tag="LP859_001777"
     CDS             168..362
                     /locus_tag="LP859_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029735.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="LP859_001778"
     CDS             631..930
                     /locus_tag="LP859_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029736.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="LP859_001779"
     CDS             complement(1027..1461)
                     /locus_tag="LP859_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029737.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LP859_001780"
     CDS             complement(1693..2403)
                     /locus_tag="LP859_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL3029738.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LP859_001781"
     CDS             complement(2393..3253)
                     /locus_tag="LP859_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL3029739.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LP859_001782"
     CDS             complement(3258..3896)
                     /locus_tag="LP859_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL3029740.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LP859_001783"
     CDS             complement(3911..4210)
                     /locus_tag="LP859_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029741.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LP859_001784"
     CDS             complement(4253..5737)
                     /locus_tag="LP859_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL3029742.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LP859_001785"
     CDS             complement(5757..6218)
                     /locus_tag="LP859_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3029743.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LP859_001786"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LP859_001786"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL3029744.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="LP859_001787"
     CDS             7521..8291
                     /locus_tag="LP859_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3029745.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="LP859_001788"
     CDS             complement(8564..9391)
                     /locus_tag="LP859_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3029746.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="LP859_001789"
     CDS             9959..11194
                     /locus_tag="LP859_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL3029747.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="LP859_001790"
     CDS             complement(12193..12453)
                     /locus_tag="LP859_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL3029748.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="LP859_001791"
     CDS             complement(12480..13781)
                     /locus_tag="LP859_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3029749.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="LP859_001792"
     CDS             complement(14073..14531)
                     /locus_tag="LP859_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3029750.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="LP859_001793"
     CDS             complement(14544..15875)
                     /locus_tag="LP859_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL3029751.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLIGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="LP859_001794"
     CDS             complement(15886..17328)
                     /locus_tag="LP859_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3029752.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="LP859_001795"
     CDS             complement(17315..18079)
                     /locus_tag="LP859_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL3029753.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="LP859_001796"
     CDS             complement(18183..19058)
                     /locus_tag="LP859_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3029754.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="LP859_001797"
     tRNA            complement(19313..19397)
                     /locus_tag="LP859_001797"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="LP859_001798"
     tRNA            complement(19415..19486)
                     /locus_tag="LP859_001798"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="LP859_001799"
     tRNA            complement(19496..19568)
                     /locus_tag="LP859_001799"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="LP859_001800"
     tRNA            complement(19630..19714)
                     /locus_tag="LP859_001800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="LP859_001801"
     tRNA            complement(19735..19805)
                     /locus_tag="LP859_001801"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="LP859_001802"
     tRNA            complement(19851..19922)
                     /locus_tag="LP859_001802"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="LP859_001803"
     tRNA            complement(19934..20006)
                     /locus_tag="LP859_001803"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="LP859_001804"
     tRNA            complement(20009..20082)
                     /locus_tag="LP859_001804"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="LP859_001805"
     tRNA            complement(20089..20169)
                     /locus_tag="LP859_001805"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="LP859_001806"
     tRNA            complement(20171..20246)
                     /locus_tag="LP859_001806"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="LP859_001807"
     tRNA            complement(20282..20354)
                     /locus_tag="LP859_001807"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="LP859_001808"
     tRNA            complement(20364..20436)
                     /locus_tag="LP859_001808"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="LP859_001809"
     tRNA            complement(20452..20523)
                     /locus_tag="LP859_001809"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="LP859_001810"
     tRNA            complement(20538..20627)
                     /locus_tag="LP859_001810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6531 a         4318 c         3275 g         6540 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ttaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DAFEZB010000047        20605 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFEZB010000047
VERSION     DAFEZB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.047"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(51..152)
                     /locus_tag="LP859_001811"
                     /pseudo
     CDS             complement(51..152)
                     /locus_tag="LP859_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            415..1716
                     /locus_tag="LP859_001812"
                     /pseudo
     CDS             415..1716
                     /locus_tag="LP859_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1914..2510)
                     /locus_tag="LP859_001813"
     CDS             complement(1914..2510)
                     /locus_tag="LP859_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HBL3029755.1"
                     /translation="MKTVKKMIKLVFASLGIIVFLGACSNQSESNNSKSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            complement(2531..2737)
                     /locus_tag="LP859_001814"
     CDS             complement(2531..2737)
                     /locus_tag="LP859_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029756.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            complement(2752..4146)
                     /locus_tag="LP859_001815"
     CDS             complement(2752..4146)
                     /locus_tag="LP859_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3029757.1"
                     /translation="MRKRTIKTQLVLSFLAIATLIIGSISLVALSLTNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKALENNIYFSIEDEQGNMVWELKGK
                     NLKSAQDKLKKNALKVSEKKSVKLDETIEAKKKLINDGNEFGKVTFYYFGPFAYTEHD
                     ALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIPQ
                     ETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGVW
                     KITPERLQSCYDEIDRLTRLIGNIEVINKIEAKYDHLNKTEFNIYKLIESVIENFASK
                     IESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVLL
                     IVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVVEAHKGSITVKSK
                     LGLGTKFVIKLPFQ"
     gene            complement(4146..4826)
                     /locus_tag="LP859_001816"
     CDS             complement(4146..4826)
                     /locus_tag="LP859_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3029758.1"
                     /translation="MKILIVDDEEKILEIIDAYLVANHYSVYKAISGAMALEKFEKNN
                     PDLVVLDLMLPDTDGLTVCRKIREVSNVPIIMLTAKSDEDDILTGLKLGADDYMIKPF
                     SPKELVARIQTVLRRTETLSNPNKLSSSNGELIVYPESRQVYLHQVELNLTTTEFDIL
                     HALISTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHIKNLRHKIENDAKSPMYILTVHG
                     TGYRFGGH"
     gene            complement(4854..6437)
                     /locus_tag="LP859_001817"
     CDS             complement(4854..6437)
                     /locus_tag="LP859_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HBL3029759.1"
                     /translation="MWGNQHKISTYFFYGNYIIDMGDDGLKNNKYIWLSATISMLTLG
                     GVFILIQQQNKEEHAEVVFQEEQRQISSRMSNMMGDNQEADLKVVHQTERQLLIPPVL
                     EPSSKSKENVIYDIVAQNGEVQIMDGEKTETLGYNGDFLGPVIRLKKGQKVTINTNNN
                     LDASTSFHWHGLKVASDADGGPHQIIETGQKKSVTFEVDQEASTLWFHPHPEGETASQ
                     VYKGLAGLMYIDDDNSKSLDLPSKYGVDDIPLIVQDKSFSSTNQINYENDFNSDGTKG
                     ETLLTNGTINPYIEIKNRWMRYRIVNGSNSRNFTFTLDKDESFYQIATDGGFLNTSVK
                     LSKLLLAPGERAEILVDTQNYKKGEVIHLLANNLVALTMRIENTIENKEFTPSDSLNT
                     ISTLDEKKLEDLARQSINLRGMSHMVNINNKQFDMERIDLYKKLGTQEIWEVNNISSM
                     MGGMIHPFHIHGVQFQILSRDGNQPALNEQGWKDTVLVNPDETVELLVKFDREGIFMY
                     HCHILEHEEYGMMGQMEIK"
     gene            complement(6448..6672)
                     /locus_tag="LP859_001818"
     CDS             complement(6448..6672)
                     /locus_tag="LP859_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL3029760.1"
                     /translation="MMKYLQIVVIDGMNCSRCASKVRNALEAEPTVSVKIVDLARGIV
                     TFDADSKLTLTFLNQLLKNTKYTVTRNISI"
     gene            complement(7305..7523)
                     /locus_tag="LP859_001819"
     CDS             complement(7305..7523)
                     /locus_tag="LP859_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL3029761.1"
                     /translation="MTEHLQPLFISGMACLGCAGEIKYALEKNPSISVRKVNLEEGLV
                     TIATKTDLSISVINQLLKNTNYKALQVW"
     gene            complement(7540..9990)
                     /locus_tag="LP859_001820"
     CDS             complement(7540..9990)
                     /locus_tag="LP859_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL3029762.1"
                     /translation="MISKYNLYGMTCAVCATTIEKKIHELDGVYFAKVNLTTEVLKLE
                     YDEGILSNHTVITAIQDIGYDAEIRKKTEIKVFGISGMTCVSCANKIENVVKSMTYVK
                     YANVNFEAEKLSVISTDPSAVAKISDVVKILGYQLYALSEDEEQVIYKTKEQEQQQQK
                     LRRRFITSMLFTIPIIYVASANILELPLFEIIDPTTNPSFFSMTQLILTAPVILANKD
                     YYKIGFSSLMKKHPKMDILIALGTSIALLYGIFATIQIILGNYLYTKELYFGASAVIL
                     TLIRLEKYLELVTKDKTSEAIQKLMRMTPKTAKTIRHNKEQTVPIESVLVGDVIIAKP
                     GERLPLDGIVISGKSLVDESMPTGENLPVEKKANDRVIGPSKNKNGVIRYETTHIGKD
                     TTLAQISKLVDDAQSSKAPIEKLADIISSYFVYIIIVVAIGSSLLWVLSGQSISFSFS
                     VLISVLVITCPCAFGLATRTAIMVGMGKGAENGILIKSGEALETTHHVKTILFDKTGT
                     ITKGKPVVTDIILAENMDRNFLLFLAASAEKASEHPLGEAIVKEAEIVGIDLVEPSIF
                     ESFSGLGVNAILKGTEVSLGNEEFFKQLNIDISKFKLISDNLVSKGKTPVYIANDRIF
                     LGIIAIEDTIKPASALAIQKLNKLGLNVVMLTGDNKLTAQAIANQVGITNVISEVLPQ
                     EKAYYVKMLQSKEHRVAMVGNGINDALALAQADIGFVMGSGTDIAMESADIILMNDDL
                     QLLMSTIDLSKKTIWNIKSNLYWAFAYNMLSVPIAMGVLNIFWRSLLSPLIAGVAMSF
                     SSITVLLNSLRLKNFKVK"
     gene            complement(9974..10339)
                     /locus_tag="LP859_001821"
     CDS             complement(9974..10339)
                     /locus_tag="LP859_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBL3029763.1"
                     /translation="MVLVEHKIQVSNSELDVLKFIWRYEPVTCGLITHGMQERNSWHP
                     STTKTLIRRLLDKNVITFNTSKNQRYYTSLIKKQEFLETEIQRLLSGMDEGCISEVSY
                     YLNGLVKRSDTEEFNDFKI"
     gene            complement(10355..10969)
                     /locus_tag="LP859_001822"
     CDS             complement(10355..10969)
                     /locus_tag="LP859_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL3029764.1"
                     /translation="MKKSMLFLLFFVFALSLAITLTINFPGLLKINLFFQSDINFQNW
                     SRSQVNQDFRNLMGYLNNPFQKELVFDNLYVSDRGINHFKDVKFLFQLNYSLLLSTSS
                     VLLYLNRKKLVTRDQVREITSLIKWMIISVCVMALLFFDKAFVLFHQVFFDNDDWMFD
                     YRTDPIISFLPETFFFLCFLLIVTISVSTLTTIHHLFNKEERTL"
     gene            11125..11928
                     /locus_tag="LP859_001823"
     CDS             11125..11928
                     /locus_tag="LP859_001823"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL3029765.1"
                     /translation="MREPYDRVFISFGPFTIYWYAIFIVFGIIIGYFVANRRAKRAAL
                     PEDTIVDLLLYGLPVSIISARLYYVLFELPLYIDDPLSILKIWEGGLAIHGGLIGAVL
                     TGVIYCKKKNLSFWNVADVVAPSIVIGQIIGRWGNFMNQEAYGEIVDIEFLQSLKLPS
                     FIIDQMYIDGAYHHPTFLYESLWNIGVLIILLLVSRNRMFFGQIFLIYVSLYSVGRFW
                     IEGLRTDSLMLTANLRMAQVLSIVLLIGSILTYIYLKKSKEEDLHGSIT"
     gene            11912..12136
                     /locus_tag="LP859_001824"
     CDS             11912..12136
                     /locus_tag="LP859_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBL3029766.1"
                     /translation="MEALLNLLPLLLLLICPISMMFMHKGHGHAGHHHMHGEDHSKHT
                     GSENDIASLKIQTEQLKEEVATLKNLVKER"
     gene            12139..12318
                     /locus_tag="LP859_001825"
     CDS             12139..12318
                     /locus_tag="LP859_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBL3029767.1"
                     /translation="MQWLLLLLCPIMMIFMMRGMHSGSHNEKDDEQLKQDLENLKEQN
                     DILSSELSDLKSRLK"
     gene            complement(12432..13982)
                     /locus_tag="LP859_001826"
     CDS             complement(12432..13982)
                     /locus_tag="LP859_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBL3029768.1"
                     /translation="MYTNYNMNQLTLDLSTSFSPKENHVAVFIHELVEALQINDPYLF
                     GRPREYDLGAMMKVVLFAYTRETFTSRKIERLAEENLYARWLTQERVPTYRTIARFCV
                     SNDVQELTNKGLDQLTEYLRARNLIDDALFIDGTKILADANKYSFVWKKNTIGFDQMN
                     REKLLTMMTELKEAYALKQIPEGTTLTVDMIDELLTRMELRLDELENEVEATKRLSPN
                     PAKQQRRTLKNQVRKLNAKREKLLEHQTQFSIYGTRNSYSKTDHDATFMRVKEDHMKN
                     GQLKPAYNLQISTCNQFVLGYDVFQNPTDTKTLKPLLEKMKTAQKSPHYLVADAGYGS
                     ESNYRYIEDELPQHTALIPYGTMFKEQSKKWQTDDRKVMNWVYEPNEDFYIDPKGVRF
                     NFHAYRQRTDADGFVRDFKEYQAEKTDANQALIPEALTPKGNRRKITVNPSWEYHKEK
                     QKERLSTPDIQKIYGRRKVDVETVFGFMQACSGFTRYTVRGLEKVRKQTGLLITAINM
                     MKLTKIGT"
     gene            complement(14693..15976)
                     /locus_tag="LP859_001827"
     CDS             complement(14693..15976)
                     /locus_tag="LP859_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HBL3029769.1"
                     /translation="MVGVITVDNTYLTDVFNQYKLRENLLYQTWFIKSEKRLKAFKKV
                     RKYVQVLIKHVNNGTFPTDFKNSALEDIMNVIAEQTEIFKGAKHAFMWKPKLRIPDIY
                     ENRENQLAFSLCLDQILKANQEIKMLFAVNILAEKKIRGLGPAVANILYFLEPTIFPP
                     FNTAIVNGYNYLTGSKIRLGKWEDYFKLRDGMIELNCLGGLFSKDLGAISAFLFDIGK
                     LNYIVRENEEEYVRISESNTQKLIKQKLEKEDGKNVHQKIQYILATLGNEIGYHAWIA
                     SNDHNRIVDGNRLGDVSIPLLPETIYQLPNPLSRTIGLIDVIWFSKNGEPICAFEVEK
                     STSIISGMNRMDDLYSIHQENLMMYIVSPDQREEEIRAQFNRPHYMRNQNLHDHLRYI
                     LFTDLEKHANFMKKFGKDFTILAPISHYPSGELVR"
     gene            complement(16155..16562)
                     /locus_tag="LP859_001828"
     CDS             complement(16155..16562)
                     /locus_tag="LP859_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL3029770.1"
                     /translation="MNKTLRLMKPWDYFIITAVIMASFVPYLLFDTNNDENNNRESTD
                     VIAVVKIDGEVVDKFNLSQVNESFEKTYYPQKGKYNIIEVAPGKIRVKEDNSPDQIAV
                     RTGWISRPGEMSICLPHKLIVEILGVEENPLIY"
     gene            16834..18333
                     /locus_tag="LP859_001829"
     CDS             16834..18333
                     /locus_tag="LP859_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL3029771.1"
                     /translation="MKKTFISTSVIVFSLFFSNGLLAKAQTIEEKIKEKDDKIEAIVK
                     NKESAENYLTDLDNKITQLEKEYKKVLVEKNTSEKKLTEINQKITALEERIRLRNKLI
                     EEQARNAQVNQKSDSIISVMVNAESLSDAVTAAIGMTKLISASNEIMQAQIDDKENLD
                     ELKKEAEKKVTDAEKETRSLEEKENDLIEAKLTQSIKINEISASLATEQSEKAKFENQ
                     KQEAEKRREEELKAIAEEKRKEAEAAAILKAQEAEAASIIFENQKQEAEKRREKELKA
                     IAEEKRKEAEAAAILKAQEAEAASIIKDQEKDVSQLADTNDIGIGQENTDTQKEEQIP
                     DETSEQDSNSVNQNEYQDNNSSSDNTQIQSPSSSGWGAPVANIIVTSPFGGRADPTGF
                     SGSFHNGIDMGGTSSTPIMASRSGTVVQASFDGSAGNYIIIDHGDGYYSYYLHLSNYI
                     ATPGQSVSAGQTIGTMGTTGNSTGVHLHFGIATSSNWSGFVDPAPFLGI"
     gene            18336..19024
                     /locus_tag="LP859_001830"
                     /pseudo
     CDS             18336..19024
                     /locus_tag="LP859_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(19346..19552)
                     /locus_tag="LP859_001831"
     CDS             complement(19346..19552)
                     /locus_tag="LP859_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029772.1"
                     /translation="MDGEEYPYKVAFDDLIQLSNLPKDSNMKIAGEFFAPGFLVGLVI
                     SSIATGCFLFLLFLDYIKKTEDIP"
     gene            complement(19562..19858)
                     /locus_tag="LP859_001832"
     CDS             complement(19562..19858)
                     /locus_tag="LP859_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029773.1"
                     /translation="MANNLHYVTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFS
                     LNFIMIDYYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            complement(19940..20206)
                     /locus_tag="LP859_001833"
     CDS             complement(19940..20206)
                     /locus_tag="LP859_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL3029774.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
     gene            complement(20400..>20605)
                     /locus_tag="LP859_001834"
     CDS             complement(20400..>20605)
                     /locus_tag="LP859_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HBL3029775.1"
                     /translation="KVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGY
                     RNQRLAQRILDNELGCAIASEAV"
BASE COUNT         6526 a         3824 c         3302 g         6953 t
ORIGIN      
        1 acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatatca caattcctga
       61 acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct
      121 tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc ttttttctca
      181 attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccaataa gagaaccaaa
      241 aatcagaact actggatgta gttctgattt ttgattctac aatatttagt gttggtggaa
      301 aatctacaga ttttctactg acactttttt tgtaaaaaaa gatagaaaaa gagatagcct
      361 aactggtgga atttagtcgt ctaaaacaaa accataccaa gaaaggatat ctccatgtat
      421 cattgtacta gaaaattgtt aggtttaaca gatgaaaatt tattcttcga agaagaatgg
      481 ttagagacag tagaagaaga tggttttcgc accaatctta tccatgctaa acttagctat
      541 atactaagcc attgccgtaa atgtggcatc aaaaatgaag gacaaatcat caagaatggc
      601 tcacataaaa caaaagtcca acttttgcct tatcgagcaa ccaaaacaga attacgcttg
      661 gtacgtacgc gcttttactg taaagaatgt cagtcgacat tcaacgctca gaccaatttg
      721 gtagatgaaa actgctatct ctcaaaggaa ttaaaggtac aaattgcctt ggagctggcg
      781 aagaatacaa caagaaaaga aattgccgat cgctattttg tctctgacgt aaccgttctg
      841 cgtgtcctgc acgcctgttt aaaaacgtat catcctcgtt ttgatacgtt accttcggtc
      901 ctttgttttg atgagttcaa atcgatgaag tcctgcagtg gaaaaatgag ttttgttttt
      961 atgaatgggc agacccagca attaatcggt gtattggaga atcgtcgcct tgctttctta
     1021 aaaccatatt tccttaaatt cactaaaaaa gcacgagcga acgtgaaata cgtcgtgatg
     1081 gatatgaatg ccccttattt tgaacttgta aaagcggtct tcccaaacgc caaaatcgtg
     1141 accgatcgct ttcacattgt ccaacaaatc actcgtacgt taaatcagtt gcgaatcaaa
     1201 acaatgaaca ggtttcaaaa aacggaaccg acaaagtatt gacgattgaa gcgattctgg
     1261 aaattactcc ttacgcatgc ctatgatctg aatagttccg attatcaata tgatcgatcc
     1321 tttcgcagac caatgacgca aaaagcgatg gtcgatgagc ttttgagtta cgacgagcaa
     1381 ttaaccaggg cttacgagac ttgccagctc ctcctctatc actttaaaca caaggacaac
     1441 caaagttttt tcgatacgat caacagctta gatcaatgcc ttccacaatg gttttgtaag
     1501 aagctgacgt ttttaaataa atacaaatta gggattcaat atgctttaaa acccaggtat
     1561 agtaatggtg cattggaaag aacaaataat aagataaaag tgataaaacg agtagcctac
     1621 ggctatcgaa atttccacaa ctttcgagca agaatttatc tcattcaagg cttgatcttc
     1681 caagtaaaac aaaaaccagt taagcactct gcctaactgg ttatgactaa attttcagtt
     1741 gtctctctac cagcactaaa tgtcgaagag ccaaaaatct gagccagaat gaggttcgtt
     1801 ctgacccaga tttgattttg cacaactcat cagtccttat taacaaagag ctcatattcc
     1861 tcatgttgag aatacgaagg ccatgtacca gcttattggt ttagtatata aaattactca
     1921 ttattttcgt catattccat ctcatcttca gttacccaca tatgattctt aacttcttct
     1981 tgaccatcgg ttggagtgta attaacaacg taaacagttc ctgtaataga tttatctact
     2041 tttgcttcag ctcccatcat cccatccatg tgatcggcat tcaagataac agtatcgccc
     2101 tcatttgcta cagtctcagt atcttttaac tcttcttgta ctacccatcg atgattgtta
     2161 accattggtc ccccagtttt tggctcatag cttacttcat acattgtagt atcaaaggcc
     2221 cctacaactt gagcatttgc acctttcatc ccctccatat gatcgcctag cagtataaca
     2281 ttacttccca ccgggaattt agggttcgca gcatctttca ttgaagaagg aaccatacct
     2341 tcgtgattca ttccctccat actattcatt tcactagacg ctgtagaagt cgattcctcg
     2401 tttgtgcttt tagaattatt cgattcactt tgattgctac acgctcctaa aaatacaata
     2461 attccaagac ttgcaaatac taattttatc atctttttca ctgtcttcat cctcctttaa
     2521 ttaatcacat ttaggtcatc tcataaattt gtaatccaaa aacttctttt ttaattgtag
     2581 ctattgataa gtccttctta gaaaagatta ctacagaatt tgataagata tctaaatgaa
     2641 ctttttcgac actatctatc gaaatcagtt gaaaaaatat tttttttgcc caacattcac
     2701 atactagttt agcaattttt atctgttttt gaaacaaata actctcctcc tttattgaaa
     2761 tggtaacttt ataacaaatt tagttcctag acctaattta ctttttacag ttatactacc
     2821 tttatgcgct tcaacaacgc tctttacaat agctaatccg ataccttgcc cccctaaagc
     2881 tctacttctg gaggggtcag ccatataaaa tctatcaaaa atatgaagtt gctgatcttt
     2941 ttctataccg atgccgttgt cttcaacaat taaaagaaca tggtctttat ttttacttat
     3001 agaaaactta atggttccct ccttttgcgt aaatttaata gcattattca aaagattgat
     3061 aacaacttgg ttcattttat ctttatctgc agatatattg atgttatccc cttggatttc
     3121 aacatgaagg ttttttgact caattttgct ggcgaaattt tcaataacgg attcaatcaa
     3181 cttatatatg ttaaactcag ttttatttaa atgatcatat tttgcttcaa ttttgttgat
     3241 tacttctata tttccaatta aacgagttag acgatctatt tcatcataac aagattgtag
     3301 gcgctcggga gttattttcc agactccgtc aatcattcct tccacattgc ctttaagggt
     3361 agctaatgga gttcttaatt catgtgaaat atcagtagtt aatctttttc gaagtccatg
     3421 ctgtttctcc aactgattac ttaaatcatt tagggaatca atcaaggaat taatttcaat
     3481 aattgaagtt tcttgtggga ttttatcagc atattcccca cgggttaatt tatgtgtaaa
     3541 gtcgcttaca tgctttagtg gtaatcctaa tctagcagag atccacgaag caagtataaa
     3601 tgatacaaga agagctgcaa tagcaacata cattaaactt tgtttcatac tggagataaa
     3661 caaggcatcg tgctcagtat aagcaaaagg tccgaaataa taaaacgtta cttttccaaa
     3721 ctcgtttcca tcattaatta acttcttttt cgcttctatt gtttcgtcta gtttcacact
     3781 ttttttctca gaaactttca aggcattttt tttcagtttg tcctgtgctg acttcaaatt
     3841 ttttccttta agttcccaaa ccatgttgcc ttgttcgtct tctatagaaa aataaatatt
     3901 attttctaat gctttctcag aaagtgctgc aagttcctcg ctgttccatg tttctccact
     3961 atttaaccac aataaatcaa ttgtatatac atactgattc aacaaatctt cttgtcgctc
     4021 ttctacgtat ttactaaaat gattgttcgt caaacttaat gcgactaaac taatcgatcc
     4081 tattattaat gttgcgattg ctaaaaagga aagaactaat tgagttttta tcgttctttt
     4141 tctcattagt ggccaccaaa tctatatcct gtaccatgga ctgtcaaaat atacataggt
     4201 gatttagcat cattctcaat tttatgtctt aagtttttta tatgagaatc gatgctccta
     4261 tctagtccat caaattctat tcccttaact ttctctatca aatcactacg agaaaagact
     4321 ttattaggtg tggatattag ggcgtgtaga atatcaaatt cagttgttgt gaggtttaat
     4381 tccacttgat gtagataaac ttggcgactt tcaggatata ctatcaattc cccattactt
     4441 gaagaaagct tattaggatt tgataaggtc tcagttctgc gcaatacagt ttgtattctc
     4501 gcaactaatt ctttgggact aaatggttta atcatatagt catcggcacc taacttgaga
     4561 ccagtaagaa tatcatcctc atcagactta gctgttaaca ttataattgg tacatttgat
     4621 acttctctga tttttctaca cacagtcaat ccatcagtat ccggaagcat taaatctaac
     4681 acaactaaat caggattatt tttctcgaac ttttccaatg ccattgcgcc acttattgct
     4741 ttataaactg aataatgatt agcaactaga taggcatcaa taatttctaa tattttttct
     4801 tcatcgtcca caataagaat tttcatagac aatcatcctt cttaaattat tttctattta
     4861 atttccattt gtcccatcat tccatactct tcatgttcta atatatgaca atgatacatg
     4921 aaaatacctt cacggtcgaa tttcacaaga agttctactg tttcatccgg atttaccagc
     4981 acagtatctt tccatccttg ctcgtttagt gcgggctgat ttccatctct agataaaatt
     5041 tgaaactgaa caccatggat atggaacgga tgaatcatgc ctcccatcat actactaata
     5101 ttgttaactt cccaaatctc ttgagtacct aattttttat ataaatctat tctttccata
     5161 tcaaattgct tattgttgat gtttaccatg tgagacattc ctcttaaatt aatactttgt
     5221 cttgctaaat cttccaattt cttttcatct aaagtactaa tagtatttag actatcagag
     5281 ggagtaaact ctttgttttc tatcgtattc tcaattctca ttgtcaaggc aaccaaatta
     5341 tttgctagta aatgaattac ctcacctttt ttataattct gagtatccac taatatttct
     5401 gccctttccc cgggggctaa aagtagttta ctaagtttaa cagaagtatt cagaaaacca
     5461 ccatctgtag caatttggta gaatgattca tctttatcca aagtaaaagt gaagtttctt
     5521 gaattagatc cattaacaat tctatacctc atccatcggt tttttatctc aatatatgga
     5581 ttgatagtgc cattagtaag caacgtctcc ccttttgttc catcagagtt aaaatcattt
     5641 tcatagttaa tttgattagt ggagctgaaa gatttatctt gcacaatcaa aggaatgtcg
     5701 tcgacaccat atttggaagg taaatctaat gattttgagt tgtcatcatc aatatacatc
     5761 aaacctgcta aacccttata aacttgagat gctgtttctc cttccggatg gggatgaaac
     5821 cagagtgtag aagcttcttg atcaacctcg aatgttacac tttttttctg tcctgtttct
     5881 ataatctgat gtggacctcc atctgcatca gaagctactt tcaaaccgtg ccaatgaaaa
     5941 gatgtgctag catcaagatt attgtttgta ttgatagtaa ctttttgacc ttttttcagt
     6001 ctaataactg gtcctaaaaa atccccgtta tagcctaatg tttcagtttt ttctccatcc
     6061 attatttgaa cttctccgtt ttgagcaact atatcatata tcacattttc tttgcttttt
     6121 gaagatggtt caagaacagg gggaatcaat agttgtctct ccgtttggtg tactaccttc
     6181 aaatcagctt cttgattatc ccccatcata ttggacattc tacttgagat ttgtcgttgt
     6241 tcttcttgaa agacaacttc agcatgctcc tccttgttct gctgttgaat caatatgaaa
     6301 acaccgccta gggtcaacat tgatattgtt gctgataacc aaatatattt attattcttc
     6361 aacccatcat ctcccatatc tattatgtag ttaccataaa aaaaatatgt ggagattttg
     6421 tgttgattcc cccacatata cttatactta tatagatata ttcctagtta cagtatactt
     6481 tgtatttttt agtagttgat ttaagaatgt taaagttaat tttgaatcag catcgaaagt
     6541 aactatacct ctagccaaat caactatttt tacactgact gttggctctg cttccaaagc
     6601 atttcttact tttgatgcac accttgagca attcattccg tcaataacca ctatttgcaa
     6661 atatttcatc ataaaattcc ttctttctta aaaaagtcgc ctatattgga tttactaatc
     6721 taatcaatct tttaagaatt gtcttacaag tatcataagg aaaatttatg gagaaaccat
     6781 gtatagagtt tgttttagag cttttaaata taggatagat acagattgat ttgtttagtg
     6841 tatgattgca tatcatggac atatatcgct aagtatcgcg cccaaaacat aggtttcgag
     6901 tttcatgaat atcctaacag gtagaagtgc tatgatgata ttcgataagc atcctaatat
     6961 gaaatataag tttagaactg ctattttgga tagttcttcc tcctattcaa ataaataatt
     7021 gttctacata agaatttcac aatcctctct accctcctct actaccaagt gaaaatccat
     7081 ttcaactaat accaaaaacc tacattggga ttccaaagga tttttgagtt caaaaaaaca
     7141 tgaaatgcct ctcagaaatc cgtccattgg attcaacata tacttatgca caaaaagcag
     7201 tgaataaatt taaaaaactg gtttttctat atatctcaat ttgaaaaaag tagaatatag
     7261 aggaactttc ttaccataag aattcacata tttaatgtga tgattcacca aacttgtaat
     7321 gccttataat tcgtattttt caacaattga ttaataacgc taattgataa atctgtcttt
     7381 gttgcaatag ttactaaacc ttcttcgaga tttactttcc taacagaaat tgagggattc
     7441 ttttctaatg catacttaat ttctccggcg catcctagac aagccattcc actaataaat
     7501 aagggctgta aatgttcagt cataagacaa tcacctttcc tatttgactt tgaagttttt
     7561 taagcgaagt gaatttaaaa gtactgttat tgagctaaaa ctcatagcaa ctcctgcaat
     7621 taacgggctt aataaagacc tccaaaaaat gttcaaaaca cccatcgcta ttggaacact
     7681 taacatgtta taagcaaatg cccagtacaa atttgacttt atattccaaa ttgttttttt
     7741 gcttaaatca atcgtggaca ttagcaattg cagatcgtcg ttcatcaata ttatatccgc
     7801 agattccatc gctatatcag taccagaacc catcacaaaa cctatatccg cttgagcgag
     7861 ggcaagtgca tcgtttatcc cattacctac cattgcaacc ctatgttctt ttgactgcaa
     7921 cattttaaca taataggcct tttcttgagg taatacctca ctaataacgt tggtgatccc
     7981 aacttgatta gctattgcct gtgcagtaag tttattatca cccgtgagca taaccacatt
     8041 gaggccaagt ttattcaact tttgaattgc taatgcactt gctggcttta tagtgtcttc
     8101 aatcgcaatt attccgagaa agatacgatc atttgcaata tacactggtg tctttccttt
     8161 agatactaaa ttatctgaaa ttaacttaaa tttactaatg tctatattca actgcttaaa
     8221 gaactcttca tttcctaaac ttacttcggt acctttcaat atagcattaa cgcccaatcc
     8281 agagaaagat tcgaatatac taggttcaac cagatcaatc cccacgattt cggcttcttt
     8341 tacaattgcc tccccaagcg gatgctcgga tgctttttca gcagaggccg ctaaaaacaa
     8401 gagaaagttt ctatccatat tttctgcaag tattatatct gttaccacag gttttccttt
     8461 tgtaatcgta ccagttttat cgaataatat tgtttttacg tgatgagttg tttctaaagc
     8521 ttcaccactt ttaattaaga taccattttc agcgcctttt cccataccga ccataatagc
     8581 tgtccgtgta gctaaaccga atgcacaagg acatgttata actaaaactg aaattaagac
     8641 tgagaatgaa aacgaaatac tttgtccaga tagcacccaa agtagactag atccaatcgc
     8701 aactacaata atgatatata caaagtaact tgaaatgata tcagccagtt tttcgattgg
     8761 agctttcgaa gattgtgcat catccactag tttgcttatt tgagctaaag tggtgtcttt
     8821 accaatatga gttgtttcat atcttattac gccattttta tttttactgg gtcctattac
     8881 tcggtcatta gctttctttt caacaggaag attttcacct gtaggcatag attcatcaac
     8941 taaagactta ccacttatta caataccatc cagtggcaaa cgctctcctg gttttgctat
     9001 gatcacatct cctactaaaa ctgattcaat aggaacagtc tgttccttat tatgtctgat
     9061 agttttcgct gttttgggag tcatcctcat taatttttgt attgcttcag atgtcttatc
     9121 tttcgtcacc aactctaggt atttctctag tctgattaac gttaatatca ctgctgatgc
     9181 tccaaagtag agctcttttg tatagagata atttcccaat ataatctgta ttgttgcaaa
     9241 gatgccgtat aacaaagcaa tacttgtacc taaagcaatt aatatgtcca ttttcggatg
     9301 ttttttcatg agtgaggaaa atccaatttt ataatagtct ttattggcca atataaccgg
     9361 agcggtaagt attaattgcg tcatcgaaaa aaaactaggg tttgtcgtag gatcaattat
     9421 ttcaaatagg ggtaactcaa gtatatttgc actagcaaca tatataatag gaattgtaaa
     9481 tagcattgac gtgataaatc ttcgccttag cttctgttgc tgttgctctt gttcttttgt
     9541 tttatatatt acttgttcct catcttcaga aagagcatat agctgatatc caagaatttt
     9601 gacaacatca cttatttttg caaccgctga agggtcagtt gatattaccg agagtttttc
     9661 tgcttcaaaa tttacgttgg catatttgac ataagtcatt gatttaacta cattttcaat
     9721 tttgttagcg caggacacac aagtcattcc acttattcca aataccttta tctcagtttt
     9781 ttttcgaatc tcagcgtcat aaccaatgtc ttgaatagct gttataactg tatgattaga
     9841 caaaatacct tcatcatatt ccaattttag aacctcagtt gttaagttaa ctttagcgaa
     9901 atatactcca tccaactcat gaattttttt ctctattgtt gttgcgcaaa ctgcacaagt
     9961 cattccataa agattatatt ttgaaatcat tgaattcctc cgtgtcagaa cgtttaacca
    10021 atccgtttaa atagtagctt acttctgaga tacaaccttc atccattcca gaaagtaacc
    10081 tttgtatttc ggtttctaaa aattcttgct tcttaatcag tgaagtgtag tatctttggt
    10141 tttttgaagt gttaaacgtg atcacatttt tatctaacaa ccttcttatt aacgtttttg
    10201 ttgtggaggg atgccaacta ttacgctctt gcattccatg tgttattaaa ccacatgtta
    10261 ccggttcata tctccagata aactttaaaa catctaactc tgaattcgat acctgtattt
    10321 tgtgttcgac caagaccatc cctccttagg ctattcataa agtacgttct tctttattaa
    10381 acaaatgatg aattgttgtc aaagtagaaa cacttatagt tacaattaat aaaaagcata
    10441 aaaagaaaaa tgtttccggt aaaaaactga taattggatc tgtacgatag tcaaacatcc
    10501 aatcatcatt gtcaaaaaat acttgatgaa aaagcacaaa tgctttatca aaaaacagaa
    10561 gtgccattac acagactgat attatcatcc acttgattaa actagtgatt tctctgactt
    10621 gatcacgcgt tactaatttt ttcctgttta ggtataaaag aacgctactc gtagaaagaa
    10681 gcaatgaata gttaagctgg aacaaaaatt taacatcttt aaaatggttt ataccgcgat
    10741 ctgagacgta tagattatca aaaaccaatt ctttttgaaa aggattattt aaatagccca
    10801 ttaaatttct aaaatcttga ttaacttgag atctactcca gttttgaaaa tttatatcgc
    10861 tttgaaagaa taaattgatt ttgagcaaac cagggaagtt gattgtcaat gtaatcgcga
    10921 gggataaagc aaagacaaaa aataatagaa atagcatgct ttttttcatt aaattaatat
    10981 ttatattaga tcactccttt taatataatg ataaaaaaac ttgtgtagaa aatgtgtacc
    11041 aatgtatctg tcaaaaagtc tacataaaat ttccataata gcctgctata gtcaaaacaa
    11101 gttcattagg aaggagggaa gaatttgaga gaaccctacg acagagtatt tatttcgttt
    11161 ggtccattta ctatttattg gtatgccatt ttcatagtgt ttgggatcat aatcggatat
    11221 tttgttgcta atagaagagc aaagcgtgcg gcattgcctg aggatacaat tgttgatctg
    11281 ttgttatatg gattgccagt ttctattata tcggccagat tatactatgt actttttgaa
    11341 ttacctttgt acattgatga tcctttaagt atcttaaaaa tttgggaagg tggtctagca
    11401 atacatggtg gattaattgg tgctgtattg acaggggtta tttattgcaa gaagaaaaat
    11461 ctttcttttt ggaatgttgc tgacgtagtt gctcccagta tagtaattgg acaaatcatt
    11521 ggacgatggg gaaattttat gaatcaagag gcctatggag aaattgtaga catagagttc
    11581 ctgcaatctc tgaaactccc atctttcata attgatcaaa tgtatattga tggggcctac
    11641 catcatccga cttttttata tgagtctcta tggaatatag gagtactcat tattttgctt
    11701 ctggtgtcta gaaatagaat gttctttggg caaatatttt taatttatgt ttcactatat
    11761 tccgtcggga gattctggat tgaaggatta cggaccgaca gtttaatgct aacagcaaac
    11821 ttgagaatgg ctcaagtcct gtccatagtc ctattaattg gctctatatt aacttacatc
    11881 tatctcaaaa aatctaagga ggaagattta catggaagca ttacttaatc tcttaccact
    11941 actattacta ctaatttgcc ctatctcaat gatgtttatg cataaagggc acggacatgc
    12001 tgggcatcat catatgcatg gagaagatca ttcaaaacac actggttccg aaaatgatat
    12061 agcaagttta aaaatccaaa ctgagcaatt aaaagaagaa gttgctactc taaagaactt
    12121 agttaaggag cgatgacaat gcagtggtta ttacttttat tatgtccgat aatgatgata
    12181 tttatgatga ggggtatgca ctcaggatcg cataatgaaa aagatgacga acaattgaaa
    12241 caggatcttg aaaatttaaa agagcaaaat gatatcttaa gtagtgaact aagcgatttg
    12301 aagtcaaggt taaaataagt taaaggaggc gtgacataag ttacctagtt acgctctcca
    12361 aaaaacaagg agcacttgga aatcatgaga tttttcgagt gcttcttgtt tttttattca
    12421 ttttgagtag ttcaagttcc tatttttgtc aatttcatca tattgattgc cgtaatgagt
    12481 aaacccgtct gttttctgac tttctctaag cctcgaaccg tgtagcgagt gaagcccgaa
    12541 caagcctgca taaatccgaa gactgtttcc acgtcgactt ttctccgccc atatattttt
    12601 tgaatgtcag gcgttgataa ccgttctttt tgtttttctt tatgatactc ccaagacgga
    12661 tttacagtga tctttctacg attccccttt ggggttaagg cttcaggaat gagcgcttgg
    12721 ttcgcatccg ttttttctgc ttggtattct ttaaaatctc ggacaaagcc atccgcatct
    12781 gttcgttgac ggtaggcatg aaaattgaat cgtacccctt tggggtcgat gtaaaagtcc
    12841 tcattgggct cgtaaaccca attcattact ttccggtcat ctgtctgcca tttcttactt
    12901 tgttctttaa acatcgttcc ataaggaatc agtgcagtat gctgaggaag ttcatcttct
    12961 atatagcggt aattgctttc tgaaccataa cctgcatcgg cgaccaaata atgaggggac
    13021 ttttgtgcag tcttcatttt ttccagtaat ggtttcagtg ttttagtgtc tgttgggttt
    13081 tgaaagacat cgtagccgag cacaaattga ttacatgtag agatctgcag gttatacgca
    13141 ggtttaagtt ggccattttt catgtggtct tcttttacgc gcataaatgt cgcatcatga
    13201 tcggtttttg aataactgtt tctagtccca taaatagaaa actgcgtttg atgttccaga
    13261 agtttttctc gctttgcatt tagtttgcgt acttggtttt tcaacgttcg ccgttgttgt
    13321 ttcgctggat ttggcgaaag acgtttcgtt gcctcaactt cattttctag ttcatccaga
    13381 cgcagttcca tccgtgttaa cagttcatct atcatgtcta cggtcaaggt cgtcccttcg
    13441 ggaatctgtt tcagggcata tgcttctttt agttccgtca tcatagtaag caatttttcg
    13501 cgattcattt gatcgaaccc gatcgtattt ttcttccaga caaagctgta tttattcgca
    13561 tccgccaaga ttttggtccc gtcaataaac aacgcatcat caatcagatt ccgtgcacgc
    13621 agatactctg tcaactgatc taaaccttta ttggtcaact cctgtacatc gttggataca
    13681 caaaaacggg cgatagttcg ataagtcgga acccgttctt gcgtcaacca gcgagcatat
    13741 aggttttctt cagctaagcg ttcaatcttg cgacttgtaa aggtttctcg cgtatacgca
    13801 aaaagaacca ccttcatcat ggcacctaaa tcatattctc ttggtcgacc aaaaagatag
    13861 gggtcattaa tctgaagcgc ttcgaccagt tcatggataa aaacggcgac atgattttct
    13921 tttggagaaa atgaagtaga tagatccaaa gtgagttgat tcatgttata attagtatac
    13981 attttgttta gtcctttcag gattaaatga tggtgcattg gtgggaaccg gtgctccttt
    14041 ttattttacc caaaaaaccg caagaaatga acgctttctc gtcatttctt gcggttttct
    14101 tctatggact tatgtcacag tccctttaaa attcactaaa ttcatggttc agaatagaat
    14161 tgattctctc aaaatattta gtctaacttt actctttata aaacgaataa ggcatgcgtg
    14221 gtttataatc tttctttatg tatttctgac accaaagaaa atgatcctca tcattccatc
    14281 cactcgttgc attccaccaa gagtacataa catcccctgg tgtatagggt agtccataca
    14341 ccaggggatt ctagaagtaa gatttattgt ttctttttta agtgcaaata aaaccattat
    14401 cttcgatgaa tataaggaaa ataccttttt agataacatt tctaggttat gaatagatag
    14461 tattcactct atccgcaatt ttcttcaacg aaaagttact aaaatggaag catgggcagc
    14521 attaatatga tgattttctt ctggacgttg ctaagacatt caatagcaat gagcgccatt
    14581 ataaatgtgg aagctactcg taaaaaaagg gaagattgat ttccaattct gcccaaatgc
    14641 ttatttatac tacaattaac cgtattaatt tcaacttctt tgtccagaat aactaccgaa
    14701 ctagttcacc acttggataa tgagatatcg gggctaaaat tgtaaaatct ttgccaaatt
    14761 tcttcataaa attagcatgt ttctctaaat ctgtaaatag aatgtatcgt aagtgatcat
    14821 gcaaattttg attacgcata taatgtggtc tattaaattg tgctcgtatc tcctcttcac
    14881 gttgatccgg tgagacaata tacatcatca aattttcttg atgaatcgaa tacaaatcat
    14941 ccattcgatt catcccagaa attattgacg tcgatttttc aacctcaaaa gcacatatcg
    15001 gttccccatt ttttgaaaac caaataacat caatcaaacc aatcgtccta cttaacggat
    15061 ttggtaattg atagattgtt tctggtaata gaggtataga cacatctcct aagcgattcc
    15121 catctacaat tctattatga tcgtttgagg caatccaagc atgataacct atctcattac
    15181 ctaaagtagc taagatatat tggatttttt ggtgcacatt tttgccatcc tctttttcca
    15241 acttctgttt tattaatttc tgggtgttac tttctgaaat tctgacatac tcttcttcat
    15301 tttcgcgaac aatataattt agtttcccaa tatcaaataa aaaagctgag atagcaccca
    15361 aatctttcga aaaaagtcct cctaagcagt tgagttcaat cataccatca cggagtttaa
    15421 aatagtcctc ccatttacct aagcgaattt tcgagcccgt aagataatta tatccattta
    15481 caatagctgt gttaaaagga gggaaaatgg taggttctag aaaatataaa atgtttgcga
    15541 cagctggacc aagccctctg attttctttt ctgctagaat atttacagca aataacattt
    15601 taatttcttg attagcttta agaatttgat caagacaaag tgaaaaagct aactgatttt
    15661 ccctattttc ataaatatcc ggaattctca atttaggttt ccacataaat gcatgcttgg
    15721 ctcctttaaa aatttctgtt tgttctgcaa ttacattcat tatgtcctct agggctgaat
    15781 ttttaaaatc tgttgggaac gttccgttat tgacatgttt gataagtact tgtacgtatt
    15841 tcctaacttt tttaaacgct ttcaaacgtt tttcactttt gataaaccaa gtttgataca
    15901 gtaaattttc gcgtaattta tattgattaa acacatctgt taaataagta ttatccactg
    15961 taatgacacc taccatccct attaatattt tataaactcg ttagctctaa tatggctttc
    16021 tatatgcaaa attccgtaac caccttaaat gaagaataat gtaaacaggt tttagcgatt
    16081 ttagttaacg gtctttcctt agattactcc gtccatgtct ctataatagg attcgtggta
    16141 cttattagtt catactaata aattaaagga ttctcctcta ctcctaaaat ttcaacaatc
    16201 aatttatgag gcagacatat actcatttcc cctggtctac taatccagcc tgtacgaaca
    16261 gcaatttgat caggactatt atcttctttt accctgattt ttccaggagc tacttcaata
    16321 atattgtatt tccctttttg agggtaatat gttttctcaa aagactcatt aacttgtgat
    16381 aagttaaatt tgtctacaac ttctccatct attttaacaa ctgcaattac atctgtactt
    16441 tccctattat tattttcatc attattagta tcaaatagta aatatggtac gaatgatgcc
    16501 ataatgaccg cagtaataat gaaatagtcc caaggtttca tgagtctcaa tgttttattc
    16561 actaaattcg ttctcctgta ttaacttttg atatcatact ttacagttgt gtgaatttta
    16621 tgtaggtatg tatatgctcc ataaaatctc cataagtttt agttattttg aagctgtcta
    16681 ttaaattgaa atatagcata tggatcctat tactttttgt atagtaattc caatttcaat
    16741 tcctaataga acctgtagca acgataaaat agagaggaga atattatgta aaatgtttct
    16801 tcacaaataa aataagggaa gtgaaaaatc aatttgaaaa aaacatttat atctacctct
    16861 gtcattgtgt tttcgctatt ttttagtaat ggtctcttag ctaaagcaca gactatagag
    16921 gaaaaaatca aagaaaaaga tgataaaatt gaagcgattg taaaaaataa agaatcagct
    16981 gaaaattatc ttactgattt agataacaaa ataactcagc tagaaaaaga atataaaaaa
    17041 gtgctagtgg aaaaaaacac atctgagaag aaacttaccg agattaatca gaaaattact
    17101 gctcttgagg aaagaatacg ccttaggaat aaacttatcg aagagcaggc taggaatgca
    17161 caagtaaatc aaaaaagtga ctctataata tctgtcatgg taaatgccga atcattatca
    17221 gatgcagtca cagcagccat tggtatgact aagcttattt ctgctagtaa tgagattatg
    17281 caggcccaga tagatgataa agaaaattta gatgaattaa aaaaagaagc tgagaaaaaa
    17341 gttacagatg cggaaaaaga aactcgatct ctcgaagaga aagaaaatga ccttatagaa
    17401 gccaaattaa ctcaaagtat aaaaatcaat gagatatccg cttctttggc gacagagcaa
    17461 tccgaaaaag caaaatttga aaatcaaaaa caagaagctg aaaaaagaag ggaggaggag
    17521 cttaaagcta tagcagagga aaagagaaaa gaagcagaag ctgcggctat tttaaaagct
    17581 caagaggcag aagccgcatc tattatattt gaaaatcaaa aacaagaagc tgaaaaaaga
    17641 agggagaaag agcttaaagc tatagcagag gaaaagagaa aagaagcaga agctgcggct
    17701 attttaaaag ctcaagaggc agaagccgca tctattataa aagatcagga aaaagatgta
    17761 tcacaactag ccgataccaa tgacatagga ataggtcaag aaaatactga tacacagaaa
    17821 gaagaacaga taccagatga aacttcggag caagattcga actcagttaa tcaaaatgag
    17881 taccaagaca acaatagttc ttccgataat actcagattc aaagtcctag ttcttctggt
    17941 tggggcgcac ccgtagccaa cattatcgtc acaagtccat ttgggggaag agctgatcct
    18001 actggatttt ctggatcttt tcacaatggt atagatatgg gtggaacttc ttcaacgcct
    18061 atcatggcat caagatcagg aactgtagtg caggcgtcat ttgatggatc cgcaggtaat
    18121 tacattatta ttgatcacgg agatggttac tattcatatt accttcacct aagtaattat
    18181 attgctacac caggccaatc agtgagtgct ggacagacca tcggtaccat gggaactaca
    18241 ggaaattcaa ccggagttca tttgcatttt ggaatagcga ctagctcaaa ttggtctgga
    18301 ttcgtagatc ctgctccatt tctaggaatc taaaaatgat gaaaaaaatc aagcatcttt
    18361 ctttaaattt acttctaatt agcctttttc ttataggctt agttttaata tttaatttcc
    18421 aaattcgatc ttacttactg aataaacaag tccacaatat gacatagcaa attattcaca
    18481 gaaagatttg gaagacaatc tgaaaaggaa aggaaacttt gattttgatt ctgttgaatc
    18541 tatatcaaat gagttagtat taaaagcaca attcaagaat aatgatttgc ctataatagg
    18601 tggtatcgct atacacaatg taaaattatc tttaccaata tacaacggat tggacaacac
    18661 ctcgctcctt tctggggctg gaacaatgaa accagatcag cagatgggaa aacgaaatta
    18721 cgctttagca agtcatagtg ctaaagataa aactatttta ttttccccat tagagtttat
    18781 ttctctaaaa gaaaaaatat atgtaacaga cttaaagaat atttatattt accaagtgtc
    18841 ctttaaagaa aaagtggatc catcaagagt cgaagtcatt gatgatattc ctaataaaaa
    18901 tatgatcact cttgttacat gcggagattt ggctgctgta acgagattga ttgtacaagg
    18961 tacactgatc gatacagttg aatttaagaa tgctaatcaa tatatccatt ctttatttag
    19021 ctaatataaa aagtcttatc aatcctgaca gattacgagt agtacttttt attaaagaaa
    19081 gaaggtgctt tctaaattca aattggttta tggtcataat aaattaaata gggaaacgaa
    19141 cgcgattata agttcgtttc tctattttgt atgagtgtct ttaatctaat tgaaaagagc
    19201 cctttttgat ccaacctcaa gaaaattttg aaaaaatctt actatggagc taactagtaa
    19261 cgcaccctat ttaatcattt aggaatttgc ttatatcccc cttgtcattc tatagtttct
    19321 agggaagatt tagattgtga ttttcctaag gaatatcctc tgttttcttg atataatcca
    19381 gaaaaagtaa aaataaaaag cacccagttg caatactaga gattaccaag cctaccaaga
    19441 atcctggtgc gaaaaattct ccagcaattt tcatattact atcttttggt aagttagaca
    19501 attgaatcaa atcgtcaaaa gctactttgt aaggatactc ttccccgtcc acagtaaaaa
    19561 cctaacctgg ttcatagaga ataggtataa caagattatt gctttcctca ttttgaattt
    19621 ctccagaaaa gcttttctgt ttgaatgaat ctatgtccat accataattc gggaaccgtt
    19681 gcattgatgt cgccaaatta tagtaatcaa tcataataaa atttaatgag aaatcacgaa
    19741 ctacttgatt ttcttcccaa cctagcaaca attctttcgt ttgagcatca atttgatgcc
    19801 agcagggatc aaattgtcga ctttccaaat tggtcacata gtgcaaatta ttggccaagt
    19861 tgacgggaaa ataaacataa agtaactcat cttttgttac ttctagaacg tcaaatgaaa
    19921 aagaagcaat atctgaggtt taactcactt cgctttctat aatctttaca ccgctagtcg
    19981 cttgatgctg gatttcttga ttaggaatta cttggtattg gttgcttagc aaaatatccg
    20041 taataactgt accaccattc cgtttagcac ggacgttact tgttgagtac cctaatctcg
    20101 ttaacattag taaatatttt ttattgttca ttgaactaaa tcctgatact gaggggaaat
    20161 catagtagag attgtcttta tcaaaggatg cataagttga agtcactttt tttagtggtt
    20221 cccaatattc ttgatatact tcaaattctg ttagagtttg gttgattgtc ttggaatggc
    20281 aacagttttt ttgacaaatt ttataaggtg cagaacttct ttccgtatgc tattccgatt
    20341 gtccttgaca atgagcctcc tcggatgtga cgatcttcgg caggagctaa cagttaaagt
    20401 tagactgcct cgctagcgat agcacatccg agctcattat caaggattcg ctgcgccaat
    20461 ctctggttac ggtaaccaac cggtgtctcg tagccaagtg taccgtgcaa ccgaaggtgg
    20521 ttccaccaat tgacatagtc aaataactcc aaatccaatt gttgtaaggt ttcaaatgtg
    20581 tattgataga caaattctac tttca
//
LOCUS       DAFEZB010000048        20521 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFEZB010000048
VERSION     DAFEZB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.048"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(434..1747)
                     /locus_tag="LP859_001835"
     CDS             complement(434..1747)
                     /locus_tag="LP859_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL3029776.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1992..2216)
                     /locus_tag="LP859_001836"
     CDS             complement(1992..2216)
                     /locus_tag="LP859_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029777.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2236..2658)
                     /locus_tag="LP859_001837"
     CDS             complement(2236..2658)
                     /locus_tag="LP859_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL3029778.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(2800..3510)
                     /locus_tag="LP859_001838"
     CDS             complement(2800..3510)
                     /locus_tag="LP859_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029779.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3528..4024)
                     /locus_tag="LP859_001839"
                     /pseudo
     CDS             complement(3528..4024)
                     /locus_tag="LP859_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4061..4513)
                     /locus_tag="LP859_001840"
     CDS             complement(4061..4513)
                     /locus_tag="LP859_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3029780.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4648..5253)
                     /locus_tag="LP859_001841"
     CDS             complement(4648..5253)
                     /locus_tag="LP859_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL3029781.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5703..6323)
                     /locus_tag="LP859_001842"
     CDS             complement(5703..6323)
                     /locus_tag="LP859_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL3029782.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6325..7194)
                     /locus_tag="LP859_001843"
     CDS             complement(6325..7194)
                     /locus_tag="LP859_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL3029783.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(7217..7624)
                     /locus_tag="LP859_001844"
     CDS             complement(7217..7624)
                     /locus_tag="LP859_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL3029784.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7643..8932)
                     /locus_tag="LP859_001845"
     CDS             complement(7643..8932)
                     /locus_tag="LP859_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3029785.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8959..9261)
                     /locus_tag="LP859_001846"
     CDS             complement(8959..9261)
                     /locus_tag="LP859_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3029786.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(9286..11379)
                     /locus_tag="LP859_001847"
     CDS             complement(9286..11379)
                     /locus_tag="LP859_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3029787.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11868..12596)
                     /locus_tag="LP859_001848"
     CDS             complement(11868..12596)
                     /locus_tag="LP859_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL3029788.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12742..15363)
                     /locus_tag="LP859_001849"
                     /pseudo
     CDS             complement(12742..15363)
                     /locus_tag="LP859_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(15477..16808)
                     /locus_tag="LP859_001850"
     CDS             complement(15477..16808)
                     /locus_tag="LP859_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3029789.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16774..18252)
                     /locus_tag="LP859_001851"
     CDS             complement(16774..18252)
                     /locus_tag="LP859_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3029790.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18557..20392)
                     /gene="tdc"
                     /locus_tag="LP859_001852"
     CDS             complement(18557..20392)
                     /gene="tdc"
                     /locus_tag="LP859_001852"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL3029791.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         5929 a         4407 c         3442 g         6743 t
ORIGIN      
        1 atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa
       61 cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg
      121 aggaaattcc ctcaactgtt ttttgaaagg attgatttat tcagggaaga atgctgattt
      181 cacaacttct ttataaacgg tgttcactca gctgtccctc ggtattagct caaaaaacgg
      241 caaaatataa gaaactattt ttttcgtttt tttctctaat actcaggcca gaacactgat
      301 ttcacaacct ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaaa
      361 ggcaaaatat gaggaactat tttttcgttt ttttctctaa tactcaggcc agaacactga
      421 tttcacaacc tctttatttc acattcaata tccacatgaa tcccatgaat acaaagaaca
      481 gaccatacat gatgggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
      541 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
      601 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
      661 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
      721 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
      781 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
      841 aagtacctaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
      901 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
      961 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
     1021 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
     1081 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
     1141 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
     1201 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
     1261 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagca ataaatagac
     1321 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
     1381 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
     1441 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
     1501 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa atacagccga
     1561 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
     1621 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
     1681 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
     1741 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
     1801 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
     1861 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
     1921 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
     1981 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
     2041 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
     2101 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
     2161 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
     2221 tctcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
     2281 agggtccgaa aaattgaaag tcaagacgcc attgttgtct gtgatttctc ctggttcttc
     2341 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
     2401 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
     2461 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
     2521 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
     2581 catcacacgt gttttttgta tcatcgtttg acccttcttt ctatttgatt ttctttcctg
     2641 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
     2701 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
     2761 aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc
     2821 atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt
     2881 caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac
     2941 cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag
     3001 acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc
     3061 atcggaatct gcacctaaag gcaggaatga ttcgtcacct tgggcttttc ctaaatgcag
     3121 atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat
     3181 actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg
     3241 tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc
     3301 ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct
     3361 tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac
     3421 gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca
     3481 gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc
     3541 ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat
     3601 ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg
     3661 agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat
     3721 gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa
     3781 tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc
     3841 tcttgatcga tactcactag gaaaaaatct gtttctttat cttttagctc ttctgtaagt
     3901 ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtaa ttatcttttc
     3961 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
     4021 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
     4081 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
     4141 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
     4201 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
     4261 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
     4321 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
     4381 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
     4441 ctttgaattc gatattgcta atagagtcta gcgctcgggc aatcattccg atgtctcgaa
     4501 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg
     4561 ttgcaaatgc aactattttt aataaaaaaa tcaagttctt ttttaataac tagtaaagta
     4621 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
     4681 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
     4741 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
     4801 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
     4861 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
     4921 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
     4981 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
     5041 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
     5101 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
     5161 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
     5221 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
     5281 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
     5341 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
     5401 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca tcaaacgaaa
     5461 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
     5521 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
     5581 tcaaacatga gaagctgttt ctttgaaatt gctccttgtg actcggagct gaacgacagg
     5641 ctcacgacct ctcggtaaac ggtgttcaaa agccaatttt tcgatattaa gccatttctt
     5701 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
     5761 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
     5821 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
     5881 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
     5941 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
     6001 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
     6061 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
     6121 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
     6181 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
     6241 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
     6301 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
     6361 cataaacagt ccaatcttat gcttgacaat ttcttttact gctttatttg ctggaatcac
     6421 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
     6481 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
     6541 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
     6601 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
     6661 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
     6721 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
     6781 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
     6841 tgcttttgtc ttcaagatat tttctttgaa aggtagatgg gacccgtcat acatgacaga
     6901 agtatatccg ctatcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
     6961 taagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
     7021 catgccgata tatttagcgg tatcgacact tgtttgaata atgattggcg aacccatttc
     7081 ttttgctgcg cctagcatgg caggaagcat ttctaggtta tgcgtattaa aagctccaac
     7141 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
     7201 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
     7261 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
     7321 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
     7381 tattttccaa gctcttttct aaatattgga tggcttgatc gttctcatgc aattcagaaa
     7441 atttaagtcc aacttgttca taaagttccg ctaatttatg cctatcttgg gtagtggaaa
     7501 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
     7561 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
     7621 acatcatttt ccctactttc aattaaaacg ctttcaaaac tgggcttaag atccaggcat
     7681 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg
     7741 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
     7801 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
     7861 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
     7921 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acggcatttg
     7981 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
     8041 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
     8101 atatatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
     8161 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
     8221 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
     8281 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
     8341 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
     8401 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
     8461 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
     8521 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
     8581 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
     8641 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
     8701 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
     8761 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
     8821 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
     8881 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgctc atgtttttac
     8941 cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc
     9001 ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac
     9061 tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat
     9121 ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa
     9181 aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcgc cgcatacgaa
     9241 taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac
     9301 tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt
     9361 ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatggg agtaggagtc
     9421 tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat
     9481 tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc
     9541 atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg
     9601 aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc
     9661 tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt
     9721 caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc
     9781 gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat
     9841 gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg
     9901 tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat
     9961 cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag
    10021 attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc
    10081 gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg
    10141 gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat
    10201 cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac
    10261 ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg
    10321 gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc
    10381 agccatgtgc ttatagagat tttcgaataa ggcttcttcc tttagatgga aaggtacgtg
    10441 tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc
    10501 gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct
    10561 ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga
    10621 aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt
    10681 cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc
    10741 ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt cctccaaagg
    10801 aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa
    10861 gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc
    10921 tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac
    10981 cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc
    11041 ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc
    11101 aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa
    11161 tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa ttgtacggat
    11221 ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct gacgaatcgt
    11281 ccgctgactg acattgaact cacttgctag atgttttgct tttactggat attgggctgt
    11341 catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt
    11401 tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa
    11461 agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa
    11521 agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa
    11581 agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc tattttttgt
    11641 ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg agaagctgtg
    11701 acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt
    11761 acaaaatcgt gccttattgc cgataggcta tctctggaac accgtttatt cagaaaaagg
    11821 agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata
    11881 atcggttgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat
    11941 atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga
    12001 tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc
    12061 tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct
    12121 gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt
    12181 ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg
    12241 atttccaaaa actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc
    12301 gatttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct
    12361 actgacaaaa cagctaatgc aagaaacccc gtttgctcat aactagaagg acctgtactt
    12421 ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata actgccccca
    12481 tatgaacgac cgccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca
    12541 tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac
    12601 aaaatgattg ctaaataatt gattttttca ttgtcttcct cgttatcttt tattacaaaa
    12661 aactattttt atcatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
    12721 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacagatgt atgtgtcggc
    12781 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    12841 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    12901 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    12961 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    13021 ttgtttctgt acatatgaat cattatctag ctccttagct gcttggattg caagcctgat
    13081 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    13141 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    13201 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    13261 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    13321 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    13381 ctcctgatca atagcataat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    13441 cgtcttagca gacttatcca aaatgcgccg accttctttt acggcttttg cgatcgtatg
    13501 gaaattatca tctcctaaaa ccatatcagc tgcctgtttg cttacttcac tacctttgat
    13561 tcccatagcg attcctatat cggctctttt caaagcaggt gcatcattta cgccgtctcc
    13621 ggtcattccg acgatttctc catttttctg caaagctttc acgattcgca gtttgtgttc
    13681 tggtgtcgtt cgagcaaaga cgtcgacttt tggtacttgt cgaatcaact cttcttcaga
    13741 cataagatcc aaatcgatcc cttctaaaac tttagcagta tgttttaagc caatttgttc
    13801 cgcgatcgct tttgctgtat ctttgtgatc ccccgtaatc attttcactg agattcctgc
    13861 ttgttggcat tcctttacag ctttgactgc actttctttt ggcggatcaa tcaacccagc
    13921 aatccctgca aaagttaatt gggttaaaga ctcatgcgtg agttcctgtt tcgaatctac
    13981 ttttttgtaa gcaaatccta aaacacgctg accttttttc gcaaacgctg tggctgtttg
    14041 accccatgct tcttattctg aagaagaaag agttgattta gccaataata cttctggcgc
    14101 acctttgacg taaatcaccc ctcctttttc ctccgcatgc atcgttgcca tatatttgta
    14161 ttcagagctg aacggaattt ttgcttctat ttttttcaaa ggcagatcgg ctgtttcggt
    14221 gtggcgaatc agtgcaagtt ctgtcggatt gccacttaaa ttttctgttt tctgttcttc
    14281 tttcgtcttt aattcctgac aatttttcat aataaacagt acgcgagttt cttcctttgc
    14341 tgctacatcc acaactgtca tttcattttt tgtcaatgtc cctgtcttat ctgaacagat
    14401 cactgtcata gaacctaatg tttctacaga aggcagtcct ttgatgatcg ccttttcttt
    14461 tgccatttca tgtactccga cagataggat catggtcaac acagctggca atccttcggg
    14521 taccattgac accaccaaag cgatcatagc tgaaagaagg atatgccaat ccatgccata
    14581 gcggaatgtt gtaaaaacaa ctaagaacaa gatcaaacaa agaatgcctt gaaaaatttg
    14641 tttgttcaat cggttgatct tcttgatcag aggcgtcgtt tgttgttcga ccgattgcag
    14701 tgcttgattg attttaccga tttctgtcgt gtttcctgtt tctacaacga caccgactgc
    14761 agaaccagat tgtacaagcg ttcctgaaaa agccatattt ttttggtcac cgattgattc
    14821 ttctgctgcc aaaacggaat gatttttttc agatgcttct gattctcccg tcaaaatcga
    14881 ttcttctact agtagatcat gtacttcgat caaccgaaca tctgctggaa cgacgtctcc
    14941 ggcacgtaag acaatgacat ctccttttac tagttctttc gcagggattt tttcttgcat
    15001 cccgttcgat agtactatcg cttcttgtcc catcagctgc ttcaaaccat tcaacgattc
    15061 ttctgctttt ctttcttgcc aataactaac gagtccgttg acgatcacta cgaataaaat
    15121 aatacttcct tcgatgtatt cgctcgaagc aaatttcaaa aaagaggcaa tgatcaacac
    15181 gatcatcagc agatcagtaa agtgttttgc tatttttttc catcgttttg tctgctgtct
    15241 tgcttctatc tcattatatc cttgatcagt caggcgtttc tgccgttctt cttgcgacaa
    15301 gccttctgtt gatgtttgat agtttttcaa tacatcttct accgtcaatc tgtaccagtt
    15361 catcctatcc cctcctgttt aattgatact gttattatac gcagggaagt tttttcaggc
    15421 gtatcgatta tttcaggggg gtctgaaata attcgttttg cagaatgtat ctgggcctaa
    15481 ctgatatttc tattgcttat tcttttatat acaaaattta agaatacaaa ccagataacc
    15541 aataaaaaag gaataaagcg tgtcgtgctt tcgaataaca agctgaaagc aattgaagta
    15601 aaaaacacca acaaagcaag atctttatat ctttgtgtag tgttccgttt cttttcttta
    15661 ataaattgaa catgtgcaca caagatcgaa gaccacacaa atagaaaaca actcgttgtc
    15721 atgcctgcca aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg tagacaaaga
    15781 ccgacaaaaa ataacatccc tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa
    15841 agcgcacaaa accaatgggg caaacttttc tcttttgctg tttgatataa aatcctgcta
    15901 gtactgtaga ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat tagattgaca
    15961 attgctgctc cgctttggca atggatcact tgcagcatag tcactaaagg actttctttt
    16021 aaatctaaac aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa
    16081 aagaggatac gccaaggcag acagttgatt gcatttttca aattcttttg tgggtcactc
    16141 gtctcaccaa ctgttaatcc gatcatttca attccagcaa aactgaataa aaccatagga
    16201 aatgaagcaa tcattccagc aaagccaaac gggactaatt cattgagctg tccaaaattt
    16261 gctttgattc caatagactg ggtatgaaaa tctgagacac tcaatcctat acccaataag
    16321 ataaaaagga aaatccccaa gatcttgatg attgagaaaa aagattccaa ttcaccaaac
    16381 cacttgactg caacaaggtt gactgccatt aagatcatta gtgcgctacc tccgcttatc
    16441 aaagctggta catgtacaaa ccagaaatgg atatacaatc ctattgctgt taattccgca
    16501 agtccagctc caagccagca aagccaataa gtccaattga caaaaaatgc ccactttttg
    16561 cctaaataat agtcaacgaa atcactgata gagttaaatg tactatttgc taaaaataaa
    16621 tcacccatga cccgcatcaa accataaata gccaaaccgc atatcaaata aacgagaata
    16681 acagctggcc ctgctgatgc aattgccttt gctgaaccta aaaataatcc tgttccaatt
    16741 gcgccaccta atgcgattaa ctggatattt cgattagtta gagtccgttt cattgccggt
    16801 ttttccatca agttcacctt tttctttttc aagcagcatg gcgacttcct gttcccaaga
    16861 aggtttcttt attttttcaa tacagagtgg tactacaaac aaaatgacac tgatggctgt
    16921 gactgcaatt agattgatcc atgtcccagc tttcatcgtc agtccgacag aaaatccgat
    16981 tgccgcaaat aaaacaataa cgacagtcca aactccaaga ttgctctttt tctctttttt
    17041 tccaattcta aatggtcgat tcaactttgc atgtgtataa cgcaatctca aaactgcgat
    17101 ccccatgatg acatagacga tacagtagat aacagtagtt gaatttacga gcatcaaaaa
    17161 tgcttgattg acgccaggaa tcagcaaata aaaaagtgca aagattgaga taatgacaga
    17221 ctgagtcaaa ataacggatt tggatacgtc aaattgattc gtcttccaaa aattgaattt
    17281 tggtggatac gcaccttttc tagcactttc tgtcactgtc tttgctggtc ctgaggccca
    17341 agcagataat tggactgcta caccaatgaa gacaagtaaa ctaaacagat tcacaataag
    17401 atgtggcaaa ccaagaattt ggcaataaat cgaaatagat tgcgcgatat tattcaattc
    17461 catttgtcct tttggtacga catttgctac aactaaagca ttcaaaatat tgacgaaaaa
    17521 tataaaaatt aacgcggcaa atactccgcg aatatacgtt ttaactggat ttttcaaccg
    17581 agtgatatac acagaggaca tttcgatacc agtaaaaata aacatgatcg gcgcaaaata
    17641 aacaaatgag gatgcagtcg tagtatctgg tattaatttg tcccatgaaa aactgcctaa
    17701 tgttccggtt ggtacgatcc caactttgat ccacgcagca aaccctaata agagcatcat
    17761 cgtcaaaggc acgtacaatc ctaaccaaac accgactttt ccgccgattt ttgccatatc
    17821 aaatttcaaa ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa
    17881 tttgctgttc aaaccaagcg gcacattatc gataatatta ccaaacaacg gagcaagtgc
    17941 tgaagcgacc atcaccatcc caggaaacat ttggacccat aacagccatg agacaacgaa
    18001 cccccatttt tttcctagag cattcgatac ccaaagttct ggaccacctt tttggggaaa
    18061 cattccggca aattcaccag agatcaatga gattggaaaa gcgtacaata atactgcaac
    18121 cagcatgtaa aaaatcatcg tccagctcgt tgctgctaca tctggaatat ttcgtacact
    18181 tgctacaagg gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc
    18241 agtatctttc atctttattc cttctttcaa tagcgacata tgtttttgtt ttcttttatt
    18301 tttgttcaag aacagaaatg acaaggtttc atcgattcag tacaaaaccc agtatcaccg
    18361 aaacatccat tgaacgatct tagtgaactg ggtttgatac atgaaacgaa cgatccaact
    18421 tatagaacgt tttataaaaa agaatatctt ggcgaggaat atatgaaaaa cagggggaaa
    18481 tacaattgtt ttgatgaagc aaataattgt ctgttctcca tatgtctctt gttattcttt
    18541 cacacaaaaa tcagttctat tggatgattt cgtttagttt cttttccatt gctttggcga
    18601 tttcttttgt ataaaaattg aaaattcgat catcattcaa ataaggagtc atgatagccg
    18661 cacgtagcag agtgattttc tgttcttttt tccattcttc tttcgttaga cccattcttt
    18721 caacaaaacg aatcggagaa tcaccgtaat cttcttgtgt aaatgtagta tgagaagtaa
    18781 taaaccgttc accataaaca tcgccatcca tataagaaga ccgatcgaac attttttcat
    18841 ttaattcatt gatagcgttc aaatctgtac agccttgttc tttgaatacc caattgacca
    18901 tattaaaatc aggatgattc aatggataga cttcgattgt ttttccttta acagtaaaag
    18961 tcagatgatc gagaaattct ctgaatcttt gagctgcctc gattgaagcc ccaatcaatt
    19021 gaccatatcc ggtcacattc aatggcaata cacgatgtgc tgtccagact gcggcggcgg
    19081 ttgctccagc ttttgatcct tccaaaatat aagctcctaa catatctgga gcctttactg
    19141 atttttcaaa cacatatggc gcgaaataag aaatgatatt tctcatattt ttatgtttga
    19201 ttacgattcc tccagcagca taagggacat agcccatctt atgaggatct atcgtgacag
    19261 aatccgcttc tgaaatagct ctgaaccctt catatacctc tttgtcaatc gttacgcagt
    19321 ggtggaaaac atgatgttct tcaaagaatt cagctaagga agcatacggc acaaattctc
    19381 ctgcttcgtt tagaaacagt gaacgcgcat agccgccata tgcggcatca acatgtaaat
    19441 agaaatagat cccttcatct tttaaccttt ctcttaattg gacgatttta tctacactgt
    19501 ctacttgacc ttcttctgtc gttcctacga cagcaaccac acctagaatt gggatttttt
    19561 gttcagctaa ttcacgaatt gttttttcca aagcattaat atccattcga taatcctctt
    19621 gaactgggat agcaaccatc tgatctaagc ctactccgca aatatctaac gccttcatcc
    19681 aagaatagtg ttttgtttga ggcaccagcc atttccctaa tctttggata ttttttccgc
    19741 tacgggaaga agctgctttg acctcatcca tttcttcgtc cgtaaagctt tcggtcattt
    19801 ccaaaatttc ttcaacagat aaattcaaaa gttcccactc agacattttt ttcacttgtt
    19861 caggcagcac ttccttgaca gctaaaggaa tcgatttgat gcagcgggcg taccataatc
    19921 cttcaagatt agcaatagaa ccatctgctg caatgtgtcc ccatccgtct gtcatattga
    19981 ataatgaagc aaaatcctgt cctacttctg cttccatttg cgaagttgcc atagaagatt
    20041 ccaatgcaac attatttggg ttccacagca tcgcatagtt gtaagctaat aaagcaggca
    20101 tcaatgtttc agcattcatt tgtccccagt aacggcctgc tgaatgccat gggatcgatc
    20161 ctgcgcgtaa tcgctgactg acttcatcca agacctctat catatggtcg cgggtggcta
    20221 gataatctgg agtgagctta tctccttcgc taattgcagg catatctgaa ggaatataat
    20281 tttctctcca tccaagatgt tcatctacca tcttgttcaa caacatttta taaactggtc
    20341 cattttcagc tttgtcgcct ataaagacgg ccttgatatc catatctttc atttttcttc
    20401 cctccatatt ttgttggtca atgtctcgca gatacatgtg tttatctgta acacctcttc
    20461 actatagcgc caaaaatgag aaattttttt ttataacatc aaatccacgt agatttttac
    20521 a
//
LOCUS       DAFEZB010000049        20362 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFEZB010000049
VERSION     DAFEZB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.049"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            291..1430
                     /locus_tag="LP859_001853"
     CDS             291..1430
                     /locus_tag="LP859_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL3029792.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="LP859_001854"
     CDS             complement(1497..2072)
                     /locus_tag="LP859_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3029793.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="LP859_001855"
     CDS             complement(2077..3051)
                     /locus_tag="LP859_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL3029794.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="LP859_001856"
     CDS             3334..4302
                     /locus_tag="LP859_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3029795.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="LP859_001857"
     CDS             4415..4693
                     /locus_tag="LP859_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL3029796.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="LP859_001858"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="LP859_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL3029797.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="LP859_001859"
     CDS             5150..5905
                     /locus_tag="LP859_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3029798.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="LP859_001860"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="LP859_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL3029799.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="LP859_001861"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="LP859_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL3029800.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="LP859_001862"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="LP859_001862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL3029801.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="LP859_001863"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="LP859_001863"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL3029802.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="LP859_001864"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="LP859_001864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL3029803.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="LP859_001865"
     CDS             10642..11184
                     /locus_tag="LP859_001865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL3029804.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="LP859_001866"
     CDS             11207..12763
                     /locus_tag="LP859_001866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL3029805.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="LP859_001867"
     CDS             12777..13679
                     /locus_tag="LP859_001867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL3029806.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="LP859_001868"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="LP859_001868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL3029807.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="LP859_001869"
     CDS             15122..15544
                     /locus_tag="LP859_001869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL3029808.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="LP859_001870"
     CDS             16013..16243
                     /locus_tag="LP859_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL3029809.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="LP859_001871"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="LP859_001871"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL3029810.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="LP859_001872"
     CDS             17654..17830
                     /locus_tag="LP859_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL3029811.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="LP859_001873"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="LP859_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL3029812.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="LP859_001874"
     CDS             18325..18708
                     /locus_tag="LP859_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3029813.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="LP859_001875"
     CDS             18877..19761
                     /locus_tag="LP859_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3029814.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="LP859_001876"
     CDS             19875..20111
                     /locus_tag="LP859_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL3029815.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="LP859_001877"
     tRNA            20199..20279
                     /locus_tag="LP859_001877"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="LP859_001878"
     tRNA            20286..20357
                     /locus_tag="LP859_001878"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat ac
//
LOCUS       DAFEZB010000050        20329 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFEZB010000050
VERSION     DAFEZB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.050"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..209)
                     /locus_tag="LP859_001879"
     CDS             complement(<1..209)
                     /locus_tag="LP859_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3029816.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="LP859_001880"
     CDS             complement(308..622)
                     /locus_tag="LP859_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029817.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="LP859_001881"
     CDS             complement(690..1184)
                     /locus_tag="LP859_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029818.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="LP859_001882"
     CDS             complement(1177..1491)
                     /locus_tag="LP859_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029819.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1707..3098)
                     /locus_tag="LP859_001883"
     CDS             complement(1707..3098)
                     /locus_tag="LP859_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL3029820.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="LP859_001884"
     CDS             complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="LP859_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL3029821.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3698..3895)
                     /locus_tag="LP859_001885"
     CDS             complement(3698..3895)
                     /locus_tag="LP859_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029822.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3886..6564)
                     /locus_tag="LP859_001886"
     CDS             complement(3886..6564)
                     /locus_tag="LP859_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029823.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIEKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6551..6769)
                     /locus_tag="LP859_001887"
     CDS             complement(6551..6769)
                     /locus_tag="LP859_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029824.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6845..7897)
                     /locus_tag="LP859_001888"
     CDS             complement(6845..7897)
                     /locus_tag="LP859_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3029825.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8050..8376)
                     /locus_tag="LP859_001889"
     CDS             complement(8050..8376)
                     /locus_tag="LP859_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029826.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8388..8588)
                     /locus_tag="LP859_001890"
     CDS             complement(8388..8588)
                     /locus_tag="LP859_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029827.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8711..9313
                     /locus_tag="LP859_001891"
     CDS             8711..9313
                     /locus_tag="LP859_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3029828.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9368..10534
                     /locus_tag="LP859_001892"
     CDS             9368..10534
                     /locus_tag="LP859_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3029829.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            10951..14178
                     /gene="fss3"
                     /locus_tag="LP859_001893"
     CDS             10951..14178
                     /gene="fss3"
                     /locus_tag="LP859_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL3029830.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14291..14605
                     /locus_tag="LP859_001894"
     CDS             14291..14605
                     /locus_tag="LP859_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3029831.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14618..14992
                     /locus_tag="LP859_001895"
     CDS             14618..14992
                     /locus_tag="LP859_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3029832.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            15002..15337
                     /locus_tag="LP859_001896"
     CDS             15002..15337
                     /locus_tag="LP859_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL3029833.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            15420..16436
                     /locus_tag="LP859_001897"
     CDS             15420..16436
                     /locus_tag="LP859_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL3029834.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFPDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            17030..18904
                     /gene="ltrA"
                     /locus_tag="LP859_001898"
     CDS             17030..18904
                     /gene="ltrA"
                     /locus_tag="LP859_001898"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3029835.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            18976..19350
                     /locus_tag="LP859_001899"
                     /pseudo
     CDS             18976..19350
                     /locus_tag="LP859_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346898.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            19445..20120
                     /locus_tag="LP859_001900"
                     /pseudo
     CDS             19445..20120
                     /locus_tag="LP859_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            20113..20277
                     /locus_tag="LP859_001901"
     CDS             20113..20277
                     /locus_tag="LP859_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029836.1"
                     /translation="MTDKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         6603 a         3568 c         3499 g         6659 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttacccg
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac
     2281 tttcttgcac taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct
     2341 cttcctaaag taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga
     2401 acatagccaa gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt
     2461 gccaaatgat ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg
     2521 atcgcatttt gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg
     2581 tttgaataaa gattggttct cttttctaag ttttcatagt actcattatt cactgattca
     2641 agaccatgtt cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg
     2701 tcaatacgca accttttaca tgtttccatg ttaatattat taatgacaag atgactatga
     2761 aaatgatctt tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct
     2821 gcaaattcga cagctatttc gtgcatttta tcaatactta actctttaga agtactctta
     2881 tcaaacgaat gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta
     2941 cgtactaaat tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag
     3001 actgcaacac atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa
     3061 tttttcttgc gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta
     3121 aacttgcaat aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt
     3181 ctttcgtacc cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt
     3241 ctgataattt aaaaaaagag ggggcactcc gattggactc caaaaaaatg tcatctggat
     3301 aactaatcat caactttttt gctatgctat tcgcaagttc atctttaaaa atgttgtaac
     3361 ggctagcatc aaaactaaaa tgtaggctct tttctttttt atcttcagat tcttctctaa
     3421 aatatttagc aatgatattt aatgccttta ccgcttgatt caaattgtta ccaatttgat
     3481 ttaactgttt ggttaaaact accctatcat cttcagctaa caacggcggt tcatatttga
     3541 gtccctttaa ccgctcacgg caaacgtctg ctctagaaat tcccctttgt tttgcaatca
     3601 atgtcagttc aagataagtc tttttatcga cacgaaaatt taaggcctca ctatgtttgc
     3661 gatttttttc gtacattttt tgtttatcat ccattgatta tcccctcaca tttttttcta
     3721 gagagcaaca tttgaaattg ctctctattt catttttcat ctttaattct tcttgaatca
     3781 tcagttcaat ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac
     3841 ataattcgtc aagttctaag aaatcatgcg tactaatctt tagaattaat tgcatgacaa
     3901 tccccccctt ttatttctta ataaattatc gaaaagtgga ttaggatcat taagtgtatc
     3961 tctgaataac tcattgtctt cataagtgaa tcttcgtctt gattttgatc ccttatcatt
     4021 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     4081 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt
     4141 ttgaatgttc tcccgaacat ctactgtttt gaatccttga ctacttgtag aactttgtac
     4201 attttctcgg acatctactg tttttggttg acttgcactg ctaccttgca cattctctcg
     4261 gacatccact gatttaaacc cttgactact tgtagaattt tgtacatttt ctcgaacatc
     4321 tactgttttt ggttgacttg tactgctact ttgcagattt tctcgaacat caattgtctt
     4381 agtaccttga gcatttgaag aactttgtaa attctctctt acatcaacat tccgtccaac
     4441 atgaccattc agtgaacctt gaatattttc tcggacatct actgatcggc caccatgact
     4501 acttgtagaa ttttgtggat tttctttgac taatttttct aatcctgtat ttgaattttt
     4561 agcttcttga attctttctt tggtcaacgc ttcttttccg tcaatattag cgcctgtccg
     4621 cccttctaca cgtttcctta acattatttc ctcgcgattc attgcttcag gcgtgtattt
     4681 cgcttcatga cgcttctgat taattaattc ttcagcagac ttagcttgac cgtgcatagc
     4741 atttttacgt tcttcaatat cttgttggac tttttgctga attgcgcttg caggattatt
     4801 attcgattca gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct
     4861 atcggcattt tcaactttag caccatcttt tctatctgaa agacgtgttg gcacggaatc
     4921 agcatatttt ccagtttcat cttctttatt tttagctaac ataggtttgc tatttttatt
     4981 aatagcggac actgtaccat catctaaacc ttctttaaac ttattcttgg tatcattagc
     5041 tatatcacgc atactcttaa atggttttgt tgctctttca ctagccattg tagctccatc
     5101 tgaagctaaa ccggataatc cacgttcatg ggcatatccc aaagctcttc cagttttgcc
     5161 agcttttttt gatagagttt cgccgaaatt cttttctggt tttcgatctt ctccttctcc
     5221 gcttccttta gcacgattaa atccatttga tccttttcta aacagcattg cgcccattcc
     5281 aaaagcagaa gcaagttgcc catagccttc tttgagtcca atatcaacac caaagtatct
     5341 catgactgtt ccactgcctt tgattaaaac aaaaacggca cagatataag caattgtttt
     5401 tataaaaata tttgttgaga taccttgtcc tgagttcaga aaattgataa acatagcaaa
     5461 cattgataac ccaaatcctt gaaacccaac agttaagtaa cattgtagaa tatctgatag
     5521 aacaactttt gaacgctgtc ctgtctcaat atctgttgca aaaacaagaa cacctaacac
     5581 acgtttaaat gcaagttcta gtattgctgt aatgatcaca aacgcgctga acacataggc
     5641 gattgctaac gctgtcaacc caattgaaat cccccataga tttgcttggt atcggtaata
     5701 gcctgatttc aagctatcag aaaatgttga taagaagttg tcagaaaact ttgtagcaac
     5761 aaattcatta ttagaatttt caactaattc ataacgaaga ttgtccgcaa ttggattttc
     5821 tttttccaat ttctcaatca ctttatccgt taacacttgt gtgagatccg tctgttgaaa
     5881 attttttcgt gttaaattat ttcgatcctc cgttttatca atgcttgagt actgatctgt
     5941 tttattaata tatgctaaat ctgttactcc ttgttttatc aatgaccaag cgactccgtc
     6001 atctgaattt gtaatcgtag tagagtcctt ataaaatgtt ttagcaaatt gaataccact
     6061 agaaatcatg gttggcatta aaagtattaa tgctatagac atcactacgt tcattccaac
     6121 cgattttaaa tccactgttc cttttccagt aatcattttg taaccaacaa acatcaatga
     6181 aaggatcatt aatccaatga caattgtatt tacggctgat tgataaacat tatttagccc
     6241 tgtactttca atgaaattga acaaatccag cacatctgga atcatatttt gaatcgaatc
     6301 tgtcacagta aacatgcctt ttaccacagc ccatccgagc caaatcccga actgactaag
     6361 gaaatctcca ttgtactttt ttaggtaagg tctataaaga gaattattca aaaactctgt
     6421 ttgacttttt gtgtcatcaa accaattgaa aatactcgca tgagcaatat tacttgtaaa
     6481 aataatcata gctatcagaa caagaaaatt aacaaccaac caatttattt ttttattggc
     6541 aatctttttt tcaattattt tcatcgttac ctcccttagc ccattttctg aggctctcag
     6601 agagcttctg agccattttt tgattaatta tgacatgagt tacacaatta tcattttcag
     6661 ctcttaaatc gagaactata gtgttgaagc aaatagtctc gattaattcg agactatctg
     6721 tattttgatc tagacaccaa acttgatcta ttaaacgatt tttaatcaaa ataacccctc
     6781 ctatattcaa agttctcaaa tgaagtggag atacccctcc atttcatttg actcaattct
     6841 attcttattg cttatctatt ccttcgctga tttgcaattc aattagttct aacgctttat
     6901 tttttagaga agcaaagata taatcgtctt tttgtttgat acgatgatct gtttttgctc
     6961 ttcgtatcaa gtcacgtaaa gtcattgata gctgttctct aacatgatct acaaaatact
     7021 tgttattaca aatttcatag ataattttca gttgaatttt ttgttctttt tcagcagatc
     7081 taatcgcata tttgaatttt ttgtaccagt tgtcgaattg acgataatca ctattgctag
     7141 ccagttttaa gcaattcaca atactttctc cccatatttg tttaagaaga ttttcttcaa
     7201 taaatgtttc gatcaattct tcatctaatt tattattttt agttgaaagt gattcaagaa
     7261 gatcatcatt atgtgattct ttaatatctt tactatcttt ataagactta tataattcta
     7321 gttcattttt tgaactatca gcaaaattgt ttttgtctct tgttttcatt gatgtgtcgc
     7381 atagttcaaa ttttgaatta tcaacagaat attgatttgt tccatagttc aaattttgaa
     7441 ctatcaagtt tttttgtcta ttttcttgat gggttatctt attaatttta taaacatcat
     7501 ctttgcacag aacaggcttt aacaaataat aaaaatttgg gaacatttgc attttacttt
     7561 tattgaagcc attttttctt ttcacaagaa gtttaacttt ttgaagttca tctagtgctg
     7621 ataagattgt attttttgac attcccgaaa tttctacgag atttttgatt tcaaatatca
     7681 aaaaaatttt ttgattctca tcatacaagt tatttttcaa tgaaagtcta tttctatctt
     7741 ttagtaacat ataaactaac ttagaactta gtttcatttt cttatatttt tcatttacgt
     7801 ttaaaaaaat aggatattga tagtataatt ctgcatatat atcagaatta gaatagcctt
     7861 gcttttcaac taagctttca acttcttttt cattcattgt cacacctgct tctgatttta
     7921 tttgctataa tagattcaaa taaagttttc tcagaacttt attattgagt gttcccccga
     7981 tctgcaaact gacgggaaca ctctttttaa ttttttctac ttcatcaaat ttaaaaaacg
     8041 aggtgttttt taaatttgat gactcaatat ccattttttg cattcttctt ttgaaaacaa
     8101 ctccattttt tctaataaaa taactggcaa tcccattctt ttaaatttca aaaaagttgt
     8161 atatgataca ttcaaatatt tagccatctc ttttcctttt agaaatggtc tttctaatcc
     8221 ggtatcaatt cttgcttgat caatagcctc ttttgtcatt tcaaaaacat aatttttcaa
     8281 ttgattcgtt tgttgtttat ctaacattac tgtaattcct gcatccatta tttttctcct
     8341 ctcattttct atttcttcaa tcagccaact cgccaagttg tatttattta gattttcaaa
     8401 tcattaagta accaatttac caacttttga taagtagatg ctctaatact ttctttgctc
     8461 ccattttcga ttttgacata tgttttccga tttataccta tcaattttgc tgcatcgttt
     8521 ttagtaagta taagatcggc tctatgtctt cttaagcttc taatgaattg atcattaaga
     8581 gttatcattc agcgcctcct ttcaaattat tcactttgtg gtttttatct taataagcac
     8641 attttaaacc acaaaacgag taatatcaat taaaatatta ctcaatttgt ggttttcatg
     8701 ctataatagt ttggaattta ctataggggg cgttactttg aatagaataa aattttatat
     8761 tgattccagt aaaaagacca ttaaacaaat tagtacagaa actggtatta actatacaac
     8821 tcttacgaac tattaccgtg gtgagcgaaa tcctagaaat actgaaacat ggaatatcct
     8881 tgctgattat ttccatgttc ctgtaagtta tttgatggga ttagacgaaa caaattccat
     8941 agaacaacct aatcttgata gaataaaaac tgctcgttta gaaaaaaagg acaaaatagt
     9001 caagaatatt gccagtcttg actcagatta ttttactgaa ttatctaatc tatacttttc
     9061 catgacttct ttacttgaag tcaaacttca agaagatgac tccgactttc tttttttaaa
     9121 aagtattcta gaggactcct ttgaatacac gattctattt gccaaacaaa agaagtttat
     9181 taataaagct ttaaacttaa atatcgatga aagaaaagag ttagaatctg agttatttgg
     9241 caacttaacc caagtacagg ttaacaatct aaaaagagag ataacagctc gattaatatc
     9301 aaaacttcaa taaaactaat tattcaatca gccaactcgc caagttatca gattggagag
     9361 attagttatg tcaatacaaa agtacacaac aaaaagcggt gcacgatata gtgtccgctt
     9421 gtatgttcgt aaagatgaaa atggaaagca agaatactat gtaaaaaaag ggtttaaaac
     9481 tgaaaaagaa gccaagttac acgaagcaag aaaaaaggtc gagattgaag ataccggcta
     9541 tcataagcct tctaatgact tgtttataac agtgtatgaa gaatggttgc cttcttatag
     9601 gaatcgtgta caagaaacga catatcaacg tacaaaagac cttttccgct tgcatatact
     9661 accaaaattc gggaataagt ccatttctaa gattactcct gttcactgtc agaatgcagt
     9721 caatgaatgg gctttaaaat tcaagaatat caaacagctt aagtcttaca gctctcaaat
     9781 atttgagtat gccatttttg cagagctatt acaccgaaat cctatggcaa atacaaatct
     9841 accaaagcgg gaaaagaaag aaaatgaaaa ttacttttca ttagaagaac taaaagcttt
     9901 ttgggatatt ctcaataaag aagagccttt aaaacatatt ttaatcttcc agcttttgat
     9961 tactactggt atacgtaaag gagagctagc tgctcttcat tggtctgaca tttattttga
    10021 agaacagcta atgtatgtta gaaagagcta tgcaactatt cgaaatgaag agaagaatgc
    10081 aagaagaaaa actgtcagga ttcaaaaaaa tacaaaaaat atatcttccg aacgaatatt
    10141 accaattgat gcacaaacta ttgaaatgtt aaaaatctgg aaaaaagaac aagctcgaga
    10201 gcttttgcag tttggcataa atacaaatac aaaaaatcaa cttatcttta cctatgtaaa
    10261 tgcagacggt gacattaatc aacctttgca tgcggactat tcaaataata tattgaaacg
    10321 tttagagaag aaatataaat ttaagcacgt tactattcat ggattccggc acactcacgc
    10381 tactcttcta ctagaaggtg gagcaagtat caaagaaaca caagatcgat tgggtcataa
    10441 aaatgctgaa actaccctta acacttattc tcatgttact gaaaaagctc aacgaaatgc
    10501 gttagaaaaa ttcattgctt acactggttt ataatattgt cattaccttt ctcattacca
    10561 gaacgaaaaa aagacacctt ctcgcaatga gaaagtgtct gaaatcgttg gtataacaac
    10621 gattaacgtt ttgaaaactg tgaagcttta cgtgctttct taagacctgg ttttttcttc
    10681 gtatcattga ataagtttga aaaaccttga tttataaagg ttttcccgta aatatatttt
    10741 attatatttg attaaatttg aaatgtttgt gttctaaaat gtgttctaat ttaaaggcag
    10801 tatatttcgt attttagatt aaatttttag gctcattcat tctcaaatcc attctattca
    10861 gtcaataaaa attttaaaaa gactttataa acaaagatgt tcaagcgagc atcttatttt
    10921 tttgtgctta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa gaaattatgt
    10981 gtattaacta ttttgagttt ggctgttcaa gcaattgttg gagcaattgc tcctacgatt
    11041 acttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga tttagcacat
    11101 caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg
    11161 cattatgcag tgtacaacgg ctttaaacaa gatattttct gtattgaacc aggagtcccg
    11221 atttacaatg agttcactcc tggctatgag aaaaacccgt taccagatat gtccgataaa
    11281 gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac tcatattgtg
    11341 gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat aaaacgatca
    11401 gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa taaatcaagc cattgacgat
    11461 tatcagaaga aaccaacctt ccataatagt acggctaaaa cagtattagg gcaatcgact
    11521 actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc
    11581 gcaaacattg attatcgtgt gaatggtaat cagttgataa tcaccccaaa tgccaattca
    11641 aaagaaagtg gtgtgctgac gcttaagaaa tctgctggta ccggaacacc tgtcgcctat
    11701 aaaaaggctg gccaacagac tttaatggcc ggggcgattg ataaacctaa tacctacaca
    11761 atcaaaattg atgtggaaac ggagggttct ttaaagatca aaaaagtcga taaagaatcg
    11821 ggtgatgttg taccaggaac ggttttccat ttagactttg gaaaaacttt acctgcaaaa
    11881 gacgtgacta ctgacaaaga aggcattgct acattggatg agattcctca tggtacaaaa
    11941 gtaaccatta ccgaaaaatc agtaccggcg ccttatacga ttgataccac ccccatgact
    12001 accaccatta aagcaggcga aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg
    12061 caaatcattc tggataaaag cggagttgaa acagggagtg atctttggaa tgacaattat
    12121 tccttagctg gtaatacgtt tgccattcgc aaagacagtc caaccggtga aatcgttcaa
    12181 gaaatgacca cggatgaaaa cggtcatgcg gaaacaccaa aagagattgc taatgcgttg
    12241 gaattgggaa cctattatgt gaccgaaact aaagctagtc atgggttcgt gaatacgttc
    12301 aaacctgtaa aagtcgaatt gaagtatgcc aatcaaaccg tagcccttgt taccagtaac
    12361 gtaaaagggc aaaatcaaga agtcactggc gaaaccactt tgacgaaaga agacaaagat
    12421 actggggata aagcacaagg taaggcagtc tttgagggtg ctgaatacac cttgtttact
    12481 gctaaggatg gaaaagctgt taaatggagt gaggctttta aaccagagtt agcgaaggga
    12541 acgaaaggtt ctgatgaaac ggtcactttg actttagacg aaaagaacca agttgccgtt
    12601 aaacatttag ctatcaacga gtattattgg caagaaacaa aagcgcccga aggatatagc
    12661 cttgatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc
    12721 gtgattaccc gaaatgttac ggcaaaagaa caagtgattc gctttggctt tgatttcttt
    12781 aaatttgctg gatcagctgc gggcactacc gaaactggct tcaatgatct gacctttaaa
    12841 gtgtcgccat tggaagggac caatgaaatc acaggtgccg aagatgaagc gaccacagct
    12901 tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc ttatggggat
    12961 tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc attagaaatc
    13021 cgttctactt ttaaggaaaa caaagaagac tttgtgaaga gtgagtatgt ctttaccatt
    13081 accgaacaag accaaaaaca accaattaaa acggtgaccg ttccttacga gaagctgacg
    13141 aacaaagcat tttctgttag tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa
    13201 gatagtttaa cttctcttgc gacttggaaa gacggaaaca aagaattaac ttctctggat
    13261 tctaccgagc tagttgataa attgagttac aatctacatg aaatcaaaga agactggtat
    13321 gtggtagcgc aagccattga tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa
    13381 gccaaaccag tggtgattgc agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga
    13441 acttggaaaa ttcagcataa attaaccgcc gaacaagttt taaataaaac tattgtctta
    13501 ttcaactatg tgtatgaaaa taaggaagct tttgaagcag gcgataagcc agtagcgaag
    13561 gatgttagct tgaataatca agcccaaact gttaattgta cggtggaacg tcatgtttct
    13621 atccaaacaa aggcccacct agaaaacggt tctcagacct ttactcatgg tgatgtggtg
    13681 gatatgtttg atgatgtgtc gattactcat gatgtactgg atggctcaaa agaagctttc
    13741 gaaacaattc tgtatgcttt attaccggat ggtacaaaca aagaaatttg gaaatctggc
    13801 aagattgatt atgaagtaaa tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta
    13861 gataccggaa aatatccaga aggaaccaag tttacttttg cggaaatcaa ttacgataaa
    13921 gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca aaccttaaca
    13981 ccaaaagaag ttccaaccat actaagtacg ccgaaacaac cggaaacacc aactgttcca
    14041 agtggctctc aagaatctag tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat
    14101 tccaatgttt tactgttcat tggttttact ttgatttttg cgacggcggg ctattatttc
    14161 tggaatcgcc ggaactaagg tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg
    14221 cgaccgcctc ctaaaaaagt agtcgcgcta tttttgtatc caaaactaac aatgaaaaga
    14281 ggaaaagaag atggaattga aatttgtcgt gccagatatg gctgaaacct ttggaaagtt
    14341 aagttatgcc ggtgaaggag aaattataac agaaggatat ggacggaata ctacggtgat
    14401 tggtcgcagt tatcatttgt attccagtaa gcaacgggcc gatgatattg aggtggtagt
    14461 agctgcggaa gccggtgaaa aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc
    14521 ccatttggtt gccaaaggct atgagattga gaatcgtggg tttactgatt acgtgttgta
    14581 tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag aaggtattgt
    14641 cgtagattca ggtttaacat ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg
    14701 gaagcaaaat gaggatggaa caattggtaa tgaggtaaaa gaacgaacct ataatttaaa
    14761 atcctctgca caagggcgaa tgattcaagt tagtatccca gctaatgtac ccttacgcga
    14821 gtttgcttat gatgcagagg tagagttggt caatccaatc gtggataccg ttgccaatta
    14881 tgtttttcga gaaggaacta ccgttaattg gttcattaag gcggatgatt tagtcttgaa
    14941 gcggcagccg aatcaaggaa atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat
    15001 tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa aaggcgagct
    15061 gactgaggaa caactcgtta aagcattaac ctctgatgaa aaaatgtttc tgattataca
    15121 tcaaggttta cttggagaaa atagcgacag tgaagaagac tctgatgtac tagactggtt
    15181 aggagaagaa gagtctttct ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc
    15241 agaatccatt ttggaagaaa ttggtgtgga gatgccggat gctattgaag gctttttaaa
    15301 tcaattggtt gaaacgaaac aactcccctc ctcgtagcag taaatgacta gcataacttt
    15361 ttgaaattgc ctaatgaaat cctattgggc tttttcgatt ggaggtggaa accatcaaaa
    15421 tgtacaaagg acatcgcata cgcgcaggag atcagcactt ggtttatcac tttgttcttg
    15481 gctggctact cgctctgttc atcggttgga tgagcgtctt ttattttcaa gagtttagac
    15541 aatttgatat ttctaagcta tcactttcta ctattgaaat cgtttggtct ataaaagatt
    15601 tagtatgtct gcttggaagt ctcgcttttt caggagctat gatattgctt tatattcact
    15661 tttttcctga tcattggcga agcttgtggc atcgacaaaa gctagctcgg atgattctag
    15721 agaaccattg gtatgaagtg aaacaaactc aaagtgaagg ctttttcaaa gatttgaata
    15781 gtagccgaac cagagagact atcagttact tccccaaaat ctattatcga atgaaagatg
    15841 gattactgtc tattcgcgtt caaatttcac ttgggaaata ccaagatcag cttttaaaat
    15901 tggaaaagaa gttagaaagc ggactgtatt gtgagttagt agaaaaggaa ctcaaagact
    15961 cctatgtgga gtatacttta ctgtatgata tgatcgccaa tcgaattggg atagacgaag
    16021 tagtggcaga aaatgggact ctgcgattga tgaaaaatca agtatgggct tatgattctt
    16081 taccccatat gttaatcgct ggtggcacag gtggagggaa gacctatttc ctcctcacca
    16141 ttatcgaagc actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcag
    16201 atttagctga tttaggtacg gtgatgcctc atgtttattc acaaaaggaa gaaatttctg
    16261 cttgtgtgga agatttctat gaacgtatga tggctcgtag caaggcaatg aaagaaatgt
    16321 ctaactacaa aacaggagag aattatgcct atcttggact tccacccaac tttttaatct
    16381 ttgatgaata cgtggcttat atgggtgcga atcgttttcc tacaagcatc gaatagatgt
    16441 gaaatggaag cctattgaga ttatcaatag tgacaactaa tgttttgaga gatggaatga
    16501 aagccttcaa gttgagctga aacatccccg ctaatactcc tacatgctct aatcaatgaa
    16561 gtagattggt tttggtatga agctaggtga agtcgtctga aatctgccta agtgcttgag
    16621 cataaggctg actatagggc gggcggctga aaaagataac tatggtaaga atgtctgaaa
    16681 atgactgacg aatctgcgaa tgtacgggtc taaaaggctg atcttattga aagataggaa
    16741 catacggacg tatggtgaga taccgacaag taaggttcca tgatatgaaa atcgggatag
    16801 ctaacgaaga agctataatt tcacaggctc atagtagata cctaaggtta tacatgatag
    16861 ctaaaaacat tggaacgatg taagcagaaa actatcaatt aaatgactac ttctagatag
    16921 gtgcatataa gtaataacga aattgctttg tttctgtgaa agtaagggca cagtaccgat
    16981 gaagcgtcta acaaatgtgg agggatagcc ctaagtctat ttaataacaa tgaatgttga
    17041 aacagatgaa gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg
    17101 cttgaacaca aaaatacgtt actgggaata ttatgatcta caagaaacat tcgatcagct
    17161 atttactcaa agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa tcatttctga
    17221 aaataatatt cttttagcat accgtactat caaggcaaac aaggggtctt cgacaccagg
    17281 aacggattcc ttcacaattg ataactataa ggaaatgaac caagcagagt ttatccatct
    17341 tattctcagt catttagaaa attacaaacc aaaatccata aagcgagtaa tgatacctaa
    17401 acccaatggt gagaaacgcc cattaggaat tccatgcatg attgatcgaa tgatccaaca
    17461 gatgttcaag cagatactgg agccaatatg tgaagctaag ttttatgaac atagctacgg
    17521 tttcagacca ttgagaagtg caaagcatgc tctaggtcgc attatgtatc tcataaacat
    17581 cagtaaaatg cactatgcag tagacattga tatcaaaggt ttctttgata atgtaaacca
    17641 ccgtttactg ataaaacaat tatggaatat aggaatttgt gacaaacaag tcttagctat
    17701 tctttcaaaa tctctgaaat cacccattca aggagaaggt atcccgagca aaggaacgat
    17761 ccaaggaggt atcatttctc cactattatc taatgttgtt ctaaacgact tagaccattg
    17821 ggtctcaaag cagtggcata cctttgaaac aaagtatccc tatactaaag gttacaataa
    17881 atttcgtgct ctaagagata caaatctaaa gcaaggctat attgtcaggt acgcagatga
    17941 ctttaaaatc atgactaatg attatccgac agcattaaaa tggttccacg ctgtcaagct
    18001 ttacctaaaa gacagactta aattagatat ttccaatgag aagtctaaaa tcgtcaattt
    18061 gcgtaaatgt aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa aagggaaaaa
    18121 atgggtttgt aattcacgta tttctaacaa aaaaaaggat cagattaaag aagagattaa
    18181 acaaagaatc aaagatattc aaaagagtcc tacagcacaa aatgctctcc tgtttaatgc
    18241 tctcgtgctc ggtgttcata attatttcag gtatgccact catattaatt tagatttgaa
    18301 taggatgaac tacgttcttt cattcactac ttacaaccga ttatcaaatt gtagtacaag
    18361 atgttacccc aacaaagctt ccccaactta caaaaagttt tactcaacaa cgatgaaaac
    18421 gtataaagta gctggagtct atttgtaccc tctttgcgac gtatctacaa aaacaattta
    18481 cggatttaat acggaggata caccgtttac tccgtttggt cgtcaaagat tggagcacaa
    18541 aagcctacag tctcttgtgt tccaagaatt aagaaaattg atggaatcga aaataccgaa
    18601 tagaacggtt gagtatttag atagtagaat atctcgttac tcaatgaaat caggcaagtg
    18661 tgagattaca aaacaatttt tgccagcaaa agccgttcac tgtcatcact atttgccaaa
    18721 aagtcttggt ggagacgaca aattcgataa cttgagaatt attcataagg atatccatct
    18781 tctgatccat acaacaaata aaatgataat cgatcactac gtgcatgaac tgaaactggt
    18841 gccagagcaa atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc aaaacattca
    18901 atagaaaata aatagatgga acgccgtatg aggtgaaagt ctcacgtacg gtgtggagcg
    18961 ggggaaaaga tggagattat atcaaattct tacctatcgc tatagatgtt aacgacgaaa
    19021 gaaagtgcgg tgattttgaa taagctcaaa caaatcgtga tgttgggtcg tcaatctggt
    19081 ttctttctga ttttggcttg ccaaaggccg gatgcgaaat atttaggaga cggaatccgc
    19141 gatcaattca actttcgcgt ggctttgggt cgtatgagtg aactcggata ttccatgatg
    19201 tttggggagg tcgaaaaaaa cttctttatg aagcacatca aaggtcgagg ttatgtggat
    19261 acaggtggca gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc
    19321 ttagattcaa ttagtaaatt agtaaattag aaaagatgaa tgattgagtg atcttcaata
    19381 ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa aggggataat
    19441 taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg ataagtttat
    19501 ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt ttatggcagg
    19561 gagtcctgga gctggaaaat cagaggttgc cattggatta gctgagaatt atgataacca
    19621 tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg gaaaaaaact
    19681 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
    19741 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
    19801 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctacgtttat caagacccta
    19861 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
    19921 aggtatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
    19981 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
    20041 attatgctgc ggacaatcta gaattatttt taccactagg gtatagtagg aaagatttgg
    20101 aggttcaatt acatgactga taaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
    20161 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
    20221 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
    20281 gacaaattaa taatgtatga gccaagagca aaatgttctt ggctttttt
//
LOCUS       DAFEZB010000051        20074 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFEZB010000051
VERSION     DAFEZB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.051"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(266..1633)
                     /gene="nhaC"
                     /locus_tag="LP859_001902"
     CDS             complement(266..1633)
                     /gene="nhaC"
                     /locus_tag="LP859_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL3029837.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1742..3163)
                     /locus_tag="LP859_001903"
     CDS             complement(1742..3163)
                     /locus_tag="LP859_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3029838.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3406..5283)
                     /gene="tdc"
                     /locus_tag="LP859_001904"
     CDS             complement(3406..5283)
                     /gene="tdc"
                     /locus_tag="LP859_001904"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL3029839.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5574..6830)
                     /locus_tag="LP859_001905"
     CDS             complement(5574..6830)
                     /locus_tag="LP859_001905"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL3029840.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6942..7147)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7410..8711
                     /locus_tag="LP859_001906"
     CDS             7410..8711
                     /locus_tag="LP859_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL3029841.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8780..9379)
                     /locus_tag="LP859_001907"
     CDS             complement(8780..9379)
                     /locus_tag="LP859_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3029842.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9633..10514
                     /locus_tag="LP859_001908"
     CDS             9633..10514
                     /locus_tag="LP859_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029843.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10527..12137
                     /locus_tag="LP859_001909"
     CDS             10527..12137
                     /locus_tag="LP859_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL3029844.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12214..12873)
                     /locus_tag="LP859_001910"
     CDS             complement(12214..12873)
                     /locus_tag="LP859_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3029845.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13156..13821)
                     /locus_tag="LP859_001911"
     CDS             complement(13156..13821)
                     /locus_tag="LP859_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL3029846.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13968..14573)
                     /gene="dhaL"
                     /locus_tag="LP859_001912"
     CDS             complement(13968..14573)
                     /gene="dhaL"
                     /locus_tag="LP859_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL3029847.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14601..15611)
                     /gene="dhaK"
                     /locus_tag="LP859_001913"
     CDS             complement(14601..15611)
                     /gene="dhaK"
                     /locus_tag="LP859_001913"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL3029848.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15586..16032)
                     /gene="dhaM"
                     /locus_tag="LP859_001914"
     CDS             complement(15586..16032)
                     /gene="dhaM"
                     /locus_tag="LP859_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL3029849.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(16071..17030)
                     /locus_tag="LP859_001915"
     CDS             complement(16071..17030)
                     /locus_tag="LP859_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3029850.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            17200..17520
                     /locus_tag="LP859_001916"
     CDS             17200..17520
                     /locus_tag="LP859_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL3029851.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(17632..18501)
                     /locus_tag="LP859_001917"
     CDS             complement(17632..18501)
                     /locus_tag="LP859_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3029852.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(18597..19901)
                     /locus_tag="LP859_001918"
     CDS             complement(18597..19901)
                     /locus_tag="LP859_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3029853.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(19898..20059)
                     /locus_tag="LP859_001919"
     CDS             complement(19898..20059)
                     /locus_tag="LP859_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029854.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT         5725 a         4147 c         3663 g         6539 t
ORIGIN      
        1 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
       61 cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
      121 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
      181 acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt taaacgcttc
      241 tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc aataaaaaac
      301 cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca
      361 acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac ggtacgagag
      421 aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt gataagccaa
      481 cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga tactgttctc
      541 caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa cgagaaggtc
      601 ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat ttgatcagta
      661 acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata ctcatgatcc
      721 cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca taaccattca
      781 tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa gaaagaacga
      841 gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg attgcccccc
      901 caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt
      961 tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg
     1021 tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat
     1081 gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg
     1141 agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga
     1201 atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg
     1261 aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac
     1321 gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc
     1381 atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga
     1441 ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa
     1501 acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga
     1561 tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa
     1621 tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta
     1681 ttcacttggc aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt
     1741 tttatttttc ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat
     1801 tgatgatcaa tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac
     1861 tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc
     1921 agctgactac ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt
     1981 ctggatgttt cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat
     2041 aaataacagt tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac
     2101 cataaagcaa agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata
     2161 cgccgtattt gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta
     2221 cttggatgat tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa
     2281 taaataccat gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt
     2341 caatcaagat aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg
     2401 ctacaagcat cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc
     2461 cttttgtata attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga
     2521 tcccaacaaa aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt
     2581 cagggaaagc ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt
     2641 tgaaggctgc caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc
     2701 atacaccgat gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga
     2761 tccagtagat aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat
     2821 taccaaatgt gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga
     2881 acatttgcac ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt
     2941 ttacccataa ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca
     3001 ttgatatcgg accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac
     3061 cgactgcggc taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga
     3121 taaaggtccc aagacccatt ttacttactt cagatttact caccgttgac actctccttg
     3181 attttttgga taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca
     3241 gcgaatattt gaggggattc tttgagttta ttgcttgaag ggtttcttca aagacaacct
     3301 caaaacaaag ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc
     3361 agaaacagtt agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct
     3421 tgccaataat tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt
     3481 ttcagcatat tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg
     3541 caatacagtt acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac
     3601 aaattgtaat gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa
     3661 ctcattaccg taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa
     3721 tttattcatt gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt
     3781 gaaatctgga taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa
     3841 gtcattcaag aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc
     3901 atatcctgtg acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc
     3961 ccctgctttt gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt
     4021 ttcaaataca tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac
     4081 gataccaccg gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc
     4141 tgcttcttcg attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc
     4201 tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt
     4261 gtcttcgtct aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta
     4321 gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc
     4381 gattgctcct tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc
     4441 agctgctaga ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc
     4501 aactggcact ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca
     4561 tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct
     4621 acgtgctgag tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat
     4681 gatttcttct gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg
     4741 tgtcacttct ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc
     4801 taagtttgct aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa
     4861 ttttgcaaat tccattccta cttcttcttc catttggctt gttgcaggag aagattcata
     4921 ggcaacgtta ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa
     4981 tgtttcagag ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt
     5041 acgcatccgc gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa
     5101 actagcactg cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg
     5161 acgccagcct aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt
     5221 ctcagctttg tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact
     5281 catagtgaaa ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt
     5341 cttctattct gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat
     5401 tatccgaatt gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa
     5461 actccctcgc aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa
     5521 attggaatgt tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca
     5581 attactttgg ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg
     5641 aattcatcgg atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt
     5701 atagctccgt ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa
     5761 acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg
     5821 aatccttgcg cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc
     5881 gttatttttt gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct
     5941 gccaaacggc gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct
     6001 tcgatttctt cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca
     6061 tcttgattca accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa
     6121 acggcaccgc ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc
     6181 gttaaaccga acgcttctgc ttctggtcct tctaattttc ggattaggtc taatccagct
     6241 gtgatatttc cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc
     6301 agatgcaaaa agtcgataga ttgcaggatt tggtaagtaa actctgtaaa tgagattcct
     6361 acttccaaac ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta
     6421 ccgtagtctc tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca
     6481 aatgtgatat ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg
     6541 ttatgttgca cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg
     6601 ccgatcgttc ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat
     6661 cttttcatca tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca
     6721 acgccgcaat aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt
     6781 gtttgctgat taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc
     6841 tccgtgtagt ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc
     6901 aggataggat aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc
     6961 ggtaccaccc aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc
     7021 gcgtggcaat cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt
     7081 tgcagcgacc accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg
     7141 tcttgttatt attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa
     7201 aggatatttt ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat
     7261 tttattttca attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg
     7321 agaagtcact ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta
     7381 aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag
     7441 ccacttctgc tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat
     7501 ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga
     7561 cagatgataa gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg
     7621 accaagcgat tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc
     7681 ttcttcaaac actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga
     7741 tctctcatgt ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac
     7801 aggcacaagg atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac
     7861 aactttatca aaatgatttt gttttagtta atttgaatga tttgatccaa aaagacgaac
     7921 aaggaaatct ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt
     7981 cacaagatga tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg
     8041 tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg
     8101 gtgactttga catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt
     8161 atcgaggcgc aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa
     8221 catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa
     8281 aagtggcgga acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact
     8341 tgaatatgac tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag
     8401 aagttgcacc tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca
     8461 gcgactggca gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt
     8521 tcgatatttt ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt
     8581 tctatcgaaa tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa
     8641 aaaatcctat tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg
     8701 aatctgcttg attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca
     8761 gtttcggctt ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg
     8821 tccattctgc tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta
     8881 ccattgcttg aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt
     8941 ctttccgccc tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat
     9001 ccatcgcgtc agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat
     9061 aaaattgttt gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg
     9121 tttcttcaaa tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca
     9181 taaatgcaaa aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg
     9241 tcgctttact tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta
     9301 tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc
     9361 tcttttcaaa accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat
     9421 agttcaaatt tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa
     9481 ttccttgttt atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa
     9541 taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag
     9601 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
     9661 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
     9721 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
     9781 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
     9841 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
     9901 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
     9961 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
    10021 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
    10081 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
    10141 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
    10201 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg
    10261 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
    10321 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
    10381 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
    10441 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
    10501 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
    10561 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
    10621 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
    10681 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
    10741 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
    10801 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
    10861 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
    10921 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
    10981 tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
    11041 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
    11101 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
    11161 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
    11221 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
    11281 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
    11341 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
    11401 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
    11461 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
    11521 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
    11581 tcgtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
    11641 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
    11701 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
    11761 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
    11821 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
    11881 gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact
    11941 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
    12001 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
    12061 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
    12121 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
    12181 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
    12241 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
    12301 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
    12361 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac
    12421 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
    12481 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
    12541 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
    12601 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
    12661 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
    12721 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
    12781 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
    12841 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
    12901 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
    12961 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
    13021 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
    13081 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
    13141 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
    13201 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
    13261 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
    13321 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
    13381 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
    13441 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
    13501 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
    13561 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
    13621 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
    13681 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
    13741 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
    13801 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
    13861 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
    13921 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg
    13981 atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt
    14041 ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg
    14101 tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg
    14161 actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga
    14221 atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg
    14281 acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc
    14341 ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca
    14401 ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca
    14461 tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt
    14521 gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca
    14581 cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct
    14641 ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag
    14701 gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct
    14761 aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact
    14821 agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc
    14881 tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata
    14941 ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt
    15001 acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc
    15061 agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt
    15121 acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg
    15181 atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt
    15241 gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt
    15301 ccactatcgg cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca
    15361 tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca
    15421 tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca
    15481 acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc
    15541 atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt
    15601 ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt
    15661 gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg
    15721 ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc
    15781 cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat
    15841 cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga
    15901 tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat
    15961 aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg
    16021 tttcgctttc attaatgaac aattcaagta tataataaat ttttcgtttg tcaaattaag
    16081 ctagacaaat catctttacc tacgtaactc aaaaatactt ccaatggtgt gcgatagtgt
    16141 aatgattttc taggaatatg atttcgtttt gatgccaccg attggataaa ggtttcggat
    16201 accttattaa aatccatagc tttatataag ccatctttac gtaataagcc attcgaatgt
    16261 tcatttagtc ctcgttgaga aggtgttcct ggatcagcga aataaatcga aatatcattg
    16321 gtattactaa tctttttcca attcgagaac tcttttccac aatcaaatgt gatggattta
    16381 aacaagtgtt tcggtacaga ttgaaaccat tgattcagtc tattctcaat atctatagct
    16441 tgcctaccag cgggtttcaa ggtgatgatc actttagata atcgctcaac taacgtgatc
    16501 accgcacttt tatgttttcc accgacaatg gtgtcacctt ctaggtgacc aaactcatga
    16561 ttgaactgta tataatcagt tgttctatcc tgaatcgaac ggcgaaaagc ttgttttcct
    16621 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
    16681 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
    16741 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
    16801 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
    16861 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
    16921 gtttgtcgag aacgttgtac cagatgggct acttttgaaa cagagtgtga ttgattgaaa
    16981 taagcttcta tcattacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac
    17041 tccttataat tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt
    17101 ctagcttaat tttacaatcg gcgatttcta attttgggaa aatatgtgta gtgcttcttg
    17161 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
    17221 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
    17281 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
    17341 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
    17401 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
    17461 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
    17521 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
    17581 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
    17641 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
    17701 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
    17761 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
    17821 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
    17881 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
    17941 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
    18001 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
    18061 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
    18121 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
    18181 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
    18241 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
    18301 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
    18361 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
    18421 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
    18481 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
    18541 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
    18601 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
    18661 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
    18721 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
    18781 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
    18841 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
    18901 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
    18961 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
    19021 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
    19081 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
    19141 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
    19201 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
    19261 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
    19321 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
    19381 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
    19441 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
    19501 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
    19561 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
    19621 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc ttcgatcaca
    19681 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
    19741 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
    19801 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
    19861 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
    19921 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
    19981 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
    20041 attcttgtct ttttttcaat ttttcatctt cata
//
LOCUS       DAFEZB010000052        19366 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFEZB010000052
VERSION     DAFEZB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.052"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            99..746
                     /locus_tag="LP859_001920"
     CDS             99..746
                     /locus_tag="LP859_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL3029855.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="LP859_001921"
     CDS             1139..3784
                     /locus_tag="LP859_001921"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL3029856.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="LP859_001922"
     CDS             4094..5407
                     /locus_tag="LP859_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL3029857.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="LP859_001923"
     CDS             5397..6050
                     /locus_tag="LP859_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3029858.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="LP859_001924"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="LP859_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL3029859.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="LP859_001925"
     CDS             complement(6671..8023)
                     /locus_tag="LP859_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3029860.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="LP859_001926"
     CDS             complement(8089..9378)
                     /locus_tag="LP859_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL3029861.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="LP859_001927"
     CDS             complement(9465..10169)
                     /locus_tag="LP859_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL3029862.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="LP859_001928"
     CDS             10369..10635
                     /locus_tag="LP859_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3029863.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            10648..10806
                     /locus_tag="LP859_001929"
     CDS             10648..10806
                     /locus_tag="LP859_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029864.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(10803..11033)
                     /locus_tag="LP859_001930"
     CDS             complement(10803..11033)
                     /locus_tag="LP859_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL3029865.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="LP859_001931"
     CDS             11105..11260
                     /locus_tag="LP859_001931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029866.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            11417..11572
                     /locus_tag="LP859_001932"
     CDS             11417..11572
                     /locus_tag="LP859_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029867.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(11569..11793)
                     /locus_tag="LP859_001933"
     CDS             complement(11569..11793)
                     /locus_tag="LP859_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL3029868.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSPGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="LP859_001934"
     CDS             12060..12395
                     /locus_tag="LP859_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029869.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="LP859_001935"
     CDS             12628..13569
                     /locus_tag="LP859_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL3029870.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKGIK"
     gene            13571..14461
                     /locus_tag="LP859_001936"
     CDS             13571..14461
                     /locus_tag="LP859_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL3029871.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="LP859_001937"
     CDS             14504..15304
                     /locus_tag="LP859_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL3029872.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="LP859_001938"
     CDS             15319..16170
                     /locus_tag="LP859_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3029873.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="LP859_001939"
     CDS             16167..16526
                     /locus_tag="LP859_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL3029874.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="LP859_001940"
     CDS             16541..16702
                     /locus_tag="LP859_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL3029875.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="LP859_001941"
     CDS             16699..17025
                     /locus_tag="LP859_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029876.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="LP859_001942"
     CDS             17022..17837
                     /locus_tag="LP859_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBL3029877.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="LP859_001943"
     CDS             17976..18287
                     /locus_tag="LP859_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL3029878.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="LP859_001944"
     CDS             18284..18514
                     /locus_tag="LP859_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029879.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18521..18724
                     /locus_tag="LP859_001945"
     CDS             18521..18724
                     /locus_tag="LP859_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL3029880.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18721..19020
                     /locus_tag="LP859_001946"
     CDS             18721..19020
                     /locus_tag="LP859_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029881.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            19010..19174
                     /locus_tag="LP859_001947"
     CDS             19010..19174
                     /locus_tag="LP859_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029882.1"
                     /translation="MKFKIFEEDTRYKLEKELNDFAKNNEIQHISLATSKRGYANYYA
                     AVVSYVSREV"
     gene            19263..>19366
                     /locus_tag="LP859_001948"
     CDS             19263..>19366
                     /locus_tag="LP859_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL3029883.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSL"
BASE COUNT         6538 a         3199 c         3954 g         5675 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ccgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgg gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaaac ggacgaaaaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
    13381 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
    13441 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaagggg
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac aacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
    18541 agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
    18721 atgaaactaa aagacggatt ttacgctagt agtcacggca tcggcggttt aatgctagat
    18781 atgccgacaa agaaccctaa aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt
    18841 cgctgtgaag cagaagagtt catttatccg tttcgtggat atgtagaaca cgtctataat
    18901 cactcagcga tcattcgtat tgaaaacaca atggaatgcg ataagtggtt agcgaaaagc
    18961 aaagagaatt tagctgtagc gagattggtg gatatggagg ttataaacaa tgaagtttaa
    19021 aatctttgaa gaggacactc gttataaatt agaaaaagaa ttaaacgatt ttgcgaaaaa
    19081 caatgagatt cagcatatat ctttagcaac ttctaagaga ggttatgcaa attactatgc
    19141 agctgttgtg agctatgtaa gtcgagaagt gtaactcggc aaataaaaaa agccggatcg
    19201 ctccgactga ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact
    19261 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
    19321 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgat
//
LOCUS       DAFEZB010000053        19309 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFEZB010000053
VERSION     DAFEZB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.053"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            968..2218
                     /locus_tag="LP859_001949"
     CDS             968..2218
                     /locus_tag="LP859_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL3029884.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="LP859_001950"
     CDS             2237..3247
                     /locus_tag="LP859_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBL3029885.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="LP859_001951"
     CDS             3257..3910
                     /locus_tag="LP859_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3029886.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="LP859_001952"
     CDS             3929..4927
                     /locus_tag="LP859_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL3029887.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="LP859_001953"
     CDS             5003..5764
                     /locus_tag="LP859_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBL3029888.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="LP859_001954"
     CDS             5782..6888
                     /locus_tag="LP859_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3029889.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="LP859_001955"
     CDS             6878..7111
                     /locus_tag="LP859_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029890.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="LP859_001956"
     CDS             7310..7765
                     /locus_tag="LP859_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HBL3029891.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="LP859_001957"
     CDS             7779..8552
                     /locus_tag="LP859_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3029892.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="LP859_001958"
     CDS             8539..9669
                     /locus_tag="LP859_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3029893.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="LP859_001959"
     CDS             9700..10935
                     /locus_tag="LP859_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029894.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="LP859_001960"
     CDS             10960..12069
                     /locus_tag="LP859_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029895.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="LP859_001961"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="LP859_001961"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBL3029896.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="LP859_001962"
     CDS             13480..15117
                     /locus_tag="LP859_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBL3029897.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="LP859_001963"
     CDS             15219..15614
                     /locus_tag="LP859_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBL3029898.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="LP859_001964"
     CDS             15632..16363
                     /locus_tag="LP859_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3029899.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="LP859_001965"
     CDS             16387..17838
                     /locus_tag="LP859_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL3029900.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="LP859_001966"
     CDS             17864..18475
                     /locus_tag="LP859_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL3029901.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="LP859_001967"
     CDS             18657..19172
                     /locus_tag="LP859_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HBL3029902.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4218 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatattg
//
LOCUS       DAFEZB010000054        18329 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFEZB010000054
VERSION     DAFEZB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.054"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            270..539
                     /gene="rpsN"
                     /locus_tag="LP859_001968"
     CDS             270..539
                     /gene="rpsN"
                     /locus_tag="LP859_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL3029903.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            554..733
                     /gene="rpmF"
                     /locus_tag="LP859_001969"
     CDS             554..733
                     /gene="rpmF"
                     /locus_tag="LP859_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL3029904.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            765..1142
                     /locus_tag="LP859_001970"
     CDS             765..1142
                     /locus_tag="LP859_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029905.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1163..1312
                     /gene="rpmG"
                     /locus_tag="LP859_001971"
     CDS             1163..1312
                     /gene="rpmG"
                     /locus_tag="LP859_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL3029906.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1336..2319
                     /locus_tag="LP859_001972"
     CDS             1336..2319
                     /locus_tag="LP859_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL3029907.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2349..2471
                     /locus_tag="LP859_001973"
     CDS             2349..2471
                     /locus_tag="LP859_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL3029908.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2489..4012
                     /locus_tag="LP859_001974"
     CDS             2489..4012
                     /locus_tag="LP859_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL3029909.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIIGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSKHEIAQEMMAH"
     gene            4036..4275
                     /locus_tag="LP859_001975"
     CDS             4036..4275
                     /locus_tag="LP859_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029910.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4468..4929)
                     /locus_tag="LP859_001976"
     CDS             complement(4468..4929)
                     /locus_tag="LP859_001976"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3029911.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5124..5342)
                     /locus_tag="LP859_001977"
     CDS             complement(5124..5342)
                     /locus_tag="LP859_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029912.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5460..7076
                     /locus_tag="LP859_001978"
     CDS             5460..7076
                     /locus_tag="LP859_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL3029913.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7317..8657)
                     /locus_tag="LP859_001979"
                     /pseudo
     CDS             complement(7317..8657)
                     /locus_tag="LP859_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8933..9088)
                     /locus_tag="LP859_001980"
     CDS             complement(8933..9088)
                     /locus_tag="LP859_001980"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL3029914.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9384..9716)
                     /locus_tag="LP859_001981"
     CDS             complement(9384..9716)
                     /locus_tag="LP859_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3029915.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRLAISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10230..10421)
                     /locus_tag="LP859_001982"
     CDS             complement(10230..10421)
                     /locus_tag="LP859_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029916.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10515..10901)
                     /locus_tag="LP859_001983"
     CDS             complement(10515..10901)
                     /locus_tag="LP859_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3029917.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11595..12614
                     /locus_tag="LP859_001984"
                     /pseudo
     CDS             11595..12614
                     /locus_tag="LP859_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
     gene            complement(12691..13449)
                     /gene="istB"
                     /locus_tag="LP859_001985"
     CDS             complement(12691..13449)
                     /gene="istB"
                     /locus_tag="LP859_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL3029918.1"
                     /translation="MTTNHQKVLTNLDELGLLKMKEYYPHYLDSVRNQTISLTDALYN
                     LTQKQLDYQKEMIDKRAIKLARFPTIKSLEEFDFEFQPSINKHDILQLKSFHFLESST
                     NICFLGNSGVGKTHLATAIGIEACIQGVKTRFILFNDLIEKLTRASQRGTLVRTLKIF
                     TNVPLLIIDEIGYTPISKTQADWFYQLMARRYERASTIITTNIPFSMWAKNFDNTTAS
                     AAILDRLIHHSKVFKITGNSYRLKNYHLDKETIG"
     gene            complement(13465..14691)
                     /gene="istA"
                     /locus_tag="LP859_001986"
     CDS             complement(13465..14691)
                     /gene="istA"
                     /locus_tag="LP859_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL3029919.1"
                     /translation="MRKDVYEGVRFFMINDIKPNFNELGRQYNCDPRTVKKYYLEGSE
                     NSVKKDKDKPKRPSKLDPFKEIIDQKLELNCSAMAIFKFLKKIGYQGGYTIVKDYCRN
                     KKHTEIKKATLRVETVPGTAAQVDWKEDMTLHDKFGKSYTFNLFLYVLHFSKLKFITL
                     TWDRKQDTLFSCLYEAFNHTGGVPKEIWFDNMKTVVDRARTQYRKVVFNTRFYEFSKD
                     AGFEAIACRSYRPQTKGSVESLAKLVERLKPYDYEFYDAVDLISLVNTLCYDLNYEEI
                     SQSTYQRPVDLWEREEKGKLHPVNEALLTPYFEDNIVRIVTNESMVNFRKSKYSVPTK
                     YIGCEVEIEIDPTEKFIEIFYQGEVVQSHELTGKRLNYKETDMFEILKSDVMKHKDDE
                     EIYEYIQRTLTQYDEL"
     gene            14799..14990
                     /locus_tag="LP859_001987"
                     /pseudo
     CDS             14799..14990
                     /locus_tag="LP859_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            15007..15441
                     /locus_tag="LP859_001988"
     CDS             15007..15441
                     /locus_tag="LP859_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029920.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            16058..>18329
                     /gene="esp"
                     /locus_tag="LP859_001989"
     CDS             16058..>18329
                     /gene="esp"
                     /locus_tag="LP859_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL3029921.1"
                     /translation="MISKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVKAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTDNWEIRLQLDERLAKYVENIQVDSKKVAGISYRRTF
                     VRINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKNLDEIFQEIGED
                     NLKSDRLMYRIYLVSHQDNDKIVPGIESTGYFITDQDNFYNKLDVSENNPDQFKHGSV
                     STKYEKPNVQTKDGSGSTGANGAIILDHKLTKEKNFSYSVSATGTPWYANYKIDERLV
                     PYVAGIQMHMVQADRVVYDVSFESGTKVADLAIERREGHENYGVGSITNNDLTKLIDF
                     SNASPRPVVIRYVLQLTEPLDEILAEMKTAAQVEGNEPFGEDFIFDSWLSDTNKKLIQ
                     NTYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAG
                     TGHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSNKLIAKDELKVQIVS
                     PGFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTS
                     NVADSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTIQSGKPTFD
                     KPDVPLANDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGETEPITGTIPIKIVYQ
                     DGSVGTTDLAVTVSKDI"
BASE COUNT         6189 a         2810 c         3427 g         5903 t
ORIGIN      
        1 aaaagctact taaaacggta tttgtgtttg attggactaa taaaagtgaa ttcttatttt
       61 tcagagccta aactgcatat tcttttaaat gatttgatta tctctatgta ccgataaatt
      121 cgttttcaaa tagttatcta ttcatatgag ttttatttca tgttatttat tgttgacaag
      181 agcaactctt gttgataaag tatcagtgtc aaaacgtaac gattacgatt tgaggtgtct
      241 aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt gctaaagcaa
      301 agaaacaaat ggcgatgatt gaaaagtatg cagataaacg tcaggaatta aaagccgcag
      361 gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt ctgaggcttc
      421 gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg tcccgtatta
      481 agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca agttggtaaa
      541 aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga aacggttaag
      601 aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga atggtgatta
      661 tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag taattaaagg
      721 agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa attatttacc
      781 cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa tcgctagata
      841 aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg ggtttaccga
      901 cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa ggcttatcat
      961 ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag ttatttcaat
     1021 gtatcgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt tcacaattgg
     1081 aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt gaagatcgat
     1141 aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg tcgaaactgg
     1201 agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt tagaattaaa
     1261 aaagtattcg ccaaaattgc gcagacgggc aatttttaaa gaagtgaaat agctaagtgg
     1321 taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt aggggctgga
     1381 aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga agaaattatt
     1441 ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt tcatagcgag
     1501 gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag tgatttatta
     1561 gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat ttcacaaatc
     1621 atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact tttaacagcg
     1681 cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga tagtgacagt
     1741 tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatggc agatcgcatt
     1801 tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca gagagaaatc
     1861 aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac gattcaaaaa
     1921 aaggattttt ataatttgaa aaaatttaat catcccttag tttttgaaag tgattcatca
     1981 gaaaatcact ataatggcaa atactatcat caacaccatc aagtacatga gtttaaatca
     2041 attctcttaa cttcagatca agacataaaa aaagattttt tactccaatg gattgactgg
     2101 ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc tttacaagag
     2161 caggatttta tagttgccat gcaagaggta aacgaacagg ttaattttca atacaccact
     2221 cgacaaaaag accaaaccac tattattcta atcggaaaaa tgttggatga agaaaggatc
     2281 aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtagt gattgggggt
     2341 aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa agcccgaata
     2401 ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat aaaatgaaga
     2461 aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac ttattgggat
     2521 agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag tatcgaatga
     2581 taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt tcaccaaaaa
     2641 catcatcggc gatgaagcga acgtggattt aatggttcca gcgggatcag aacctcatga
     2701 ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt ttgtatatca
     2761 taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga aagctgggcc
     2821 taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag aagaagatca
     2881 tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg tttcacccaa
     2941 aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt atccgaaaaa
     3001 agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac ggttggatgc
     3061 tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacgc aacacgcagc
     3121 ctttggatat ttagctttgg attatggatt gaaacaagtt cctattgctg gactaagtcc
     3181 tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta aaaaaaataa
     3241 gatcaattat atttactttg aaaagaatgc taatgataaa attgcaaaaa ctttggccaa
     3301 cgaagctggg ataaaattgg aagtactgaa tcctcttgaa agtcttacaa aagaacaaat
     3361 ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt tagaaaaaac
     3421 aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg aaaagacagt
     3481 tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg attatacagg
     3541 cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag ttttcgatta
     3601 caaagcaaaa atcaataaag acatgacagc tgccgagtac aaagattatt atactactgg
     3661 ctataagaca gatattgata ctatcaacat taaagacaac accattgatt ttgtagtcaa
     3721 cggtgaacat catcaataca catacaaata tgttggatac aaaattttga attatgaaaa
     3781 aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg gtcgttttaa
     3841 atatattcaa tttagtgatc atggaattgc accaagtaag gcagagcatt tccatatttt
     3901 ctttggtgga gaaagtcaag aaaaactcta taatgaaatg cataattggc ctactttcta
     3961 tccagcttca ttgtctaaac atgaaattgc tcaagaaatg atggctcatt aaaatccaat
     4021 ttttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa gaatattgat
     4081 gcttatcggc gttatcgatt tgcaaagcaa atgagattaa atgggagtac attagctgat
     4141 attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa acgggatcct
     4201 gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa acggcataat
     4261 agcttggcca attaattgat aataccagag tagatcattt ttcccttatt tggatttgat
     4321 ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca aagaagactt
     4381 cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt tcaatcatct
     4441 gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag tattctcgaa
     4501 tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat ctggaaaata
     4561 aaagggcata ttattcaatg cttctgtaac tgcgaaatga ttggagaatt cttttcctct
     4621 atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt caatcatttt
     4681 ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa ggaagcatga
     4741 ttttctgtct gtcaaagtca ctaaacaagt accaccagtt ttgccagcta caatatcggc
     4801 tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggacggccat ggatcgaatg
     4861 agtaaggcaa atctttccac gagactcgac atgatttttt gtatgtcttg tttttcctcg
     4921 atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg tatcgaaaag
     4981 atcattgcaa attgcacgtt agatcgtgat aaaactaatt gaaaagtcca tatgttcatg
     5041 cttcaaacga ttcgagattt cctctggcga ccactgaaaa tcgacaaata aatgacgaac
     5101 aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt ttcttcgata
     5161 agcataattt ttttccgctt tggaaggtga atagtttttt tcttgtttct tctcagttca
     5221 tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat agaaaatcct
     5281 tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag taagatgacg atactggctc
     5341 ataattattt tccttctagg agtgttctcg acaaacttat actagttgga tatcgattat
     5401 gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag aggttcccta
     5461 tgaaaaaaat acctattaca atcttaactg gctatcttgg ttcaggaaaa acgatacttc
     5521 taaatcatat tctgtacggt gatcacggaa aggaaattgc tgtcattgta aatgacatag
     5581 gggaagtaaa tgttgatggg gtattagtcg acaagaaagg ttttaaaaga actggagaaa
     5641 aacttgtttc gatgccgaat ggatgcattt gttgtacatt acgcgaagat ttgattgttg
     5701 aactagatca attagtgcaa ttttctgact tgaatcaaat tgttattgaa gcttcaggaa
     5761 tcagcaaacc aattccaatt attcaagcaa ttatattagc taaaaaccct ttagacattg
     5821 atttgaccca acgattagag attggtgcca ttattatagt agtggatgta aaacgttttt
     5881 gggataattt cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt aatgtagttg
     5941 aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt aacatattac
     6001 ttttaaataa tagtgactta gtgtcaacca agacgattta caaaattaac ggtctaattg
     6061 ctaagttaca gtccaatgcc aaaataatta ggacaattaa aggtgaagta gacttatcga
     6121 aaattctaaa tacaaatctt tttaatatag atgagacttc ctattcagtg ggctggttca
     6181 aagagttaga gcttggctat caaaatcata ttcctgaaac agaagaatat ggtatctcaa
     6241 gttttatcta tcgaaatcgg ttactccttg acgcacaaaa atttgcccat cttacggata
     6301 aaaattttcc tggcaatatt acaagagcaa aagggtttct ttggtttgcc caacatagtg
     6361 atttttcgac gatctttgaa acatcgggta gctataaaag tattgaatca ttaaattatt
     6421 gggtagctag tttacctaaa attgagtgtc aaatgattat acaaaaaaat cccgaattga
     6481 aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt attggtatga
     6541 agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat tctcttaatg
     6601 cagataattt ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg tttagccaga
     6661 tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc ataattattg
     6721 gcattttgat tattacctct tttattccct tatttgtatt ctctatagaa aaggagaatg
     6781 aaacaactga agatgctatc aaatatgcca tcgttcgaat tgatggcaag gaaatggatc
     6841 gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg aagggacaat
     6901 ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat agtcctgatc
     6961 aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt tgtttacccc
     7021 ataagttagt tattagtatt gagaaaaaag aaagcacaga ttactacatt tactaatctg
     7081 gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat agcaggggct
     7141 ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac aggcacaagt
     7201 atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt attttggctc
     7261 ttcgtcaatg agtgttggtg aagaaattca atagaataat cgagatactc acacggctag
     7321 acagctatct tgtctagccg tgttgttgtt ctggctttag tcagttgggt agtattctta
     7381 gttttcttaa aatattttcc tcgatataaa aagattcgtt ctcggtagtt ttgaaagttt
     7441 ctaaagccat aggcaattcg ttttagtact ttgatatggt tattcaaaca ttctataggg
     7501 ccgttggaat agggaacgcg caacgctcgt ttgatttctg tttggtattt cttgaaggtc
     7561 gtgaacactg gttggtagtg ctctggcaac tccttgtagt cttcttttag gagcgcgacg
     7621 aattttggaa cagcttgtct tctaatagcg gaaagaaaat cctggtagac ttcgtagcct
     7681 cgctttaagg agtcatcata agataatagc cgatcgacta tctccgcttc tgtcagatga
     7741 tctcgaaatg acggacgcca gtagcgatgc tggcccttca attcccacgc attcttttga
     7801 agtagtctcc agtatttctt taagtgcttc gaccgacgat cccttttttt aaaggtcttc
     7861 attacttgaa ttcggtgatt ggtaaaggcc cgatcgatgt gttggctaat atgaaagcga
     7921 tcggcgacga ttcgggcatt cggaaaacgc tctttcacca atgtgcgata gggtgtatag
     7981 aaatctgaaa ccactagacg tacttgttca cggacttttc tagggtagcg caagaaatag
     8041 ccgcgtaaag gattgagttg ccgattttcc acaatatcaa tcagttgata ggtcttgccg
     8101 tccatcatga caaagctcat ggctcccgag acatttttca ctgatttgaa ttcatcgaaa
     8161 cacaccactt ctggtaacag ctggttcaaa gaaatggttt taggccgaag gctgcgaagt
     8221 attcgaatga cagtggtcgg agaaatgtgc ttcatacgtg cgatcaaaga catagaaaga
     8281 ggttcgctta atagctcaag aatggcttgt ttgactctgc gagcaatgga gcagcgacga
     8341 tcactaactg aatcttctgc taaaaaagtc tttccacagt gtttacattt gaagcgctgt
     8401 ttgttaatcc gcagaatggt cttgtagtta ctgacatcat tcaataagat ggatactttt
     8461 ttccagccct atttaatgat cgtttgttca ttttgatagt ggcgatgaaa gcattcctcc
     8521 ggttgatagg tcaatgtgcc atcaaaagca aaacatgttt cgtcattgat gcgctctttg
     8581 cgaaaggcat cttcatgaaa ggtaagattt aggtctagaa tatctagtaa ctctttggta
     8641 aactctgaat aggacatctt gaaacactac tttgaatggt ttttgtcgac tttattctaa
     8701 aggattttaa ggtgtctgtt tgcgtttatt agaaaaaaga aaaagtattg gcaacagaag
     8761 gcgtcaccaa cactagatat tatagatccc ttattttctt cgcttcaaat tgtcaaggag
     8821 tcatcataaa ttttgtatgg attcttttgt ttgggtagta gtcttctaaa taattttgga
     8881 ttaacacctt catctctatt ttacttttat atttgttgga gtcgattaat tctcatttga
     8941 ttgatcgatg aaacgcttca attactgcat tgtcaacagg agtcccagtt tggctcactg
     9001 aatgtgtgat gtggtggtcg ttcaacagct cttgataaat aaaagaccga tgctggctac
     9061 ctctatcgga atgaattacc gtaggcaatt ctccttaata aatttgtaat ggacggacga
     9121 caatctcttt tttcatgtgt gaatctacca tatagtaacg tagaatacga gttgctaagt
     9181 caatataggt ggacaaatag aggcgaccgt cattacatgg aatgtaagta atatccatca
     9241 cccaaacagc atcaatttgg ttctgttcaa attatcgatt caacattttt ttaccatttc
     9301 tttgattgtt ttttctctac catgagaaga tttgcgtcga tgaaatcctc ttgtgcataa
     9361 attcatttca cgcataagaa cagctacaac acgtttattg atttcgattc cttcatcgta
     9421 aagtttttga gtgatccctg gagatccgta tcgatctttg tgttcgtaga acactcgtct
     9481 tcctctttta gtcgtttatt ttcctgttca agaatgtaga aatctgcttt atcaggtaat
     9541 ctttttcctt gtccagcaaa agcatttttc ccgtatcgac gatattctga aacccatcgg
     9601 gaaattgcta gacgagagat attgtattct tcagcaattt tcttggtagt tacttttttc
     9661 tcaaggatat cctgtgaaat ctttagttta aatttacgat catatgtttt tctcaataga
     9721 gttcattcct tccggtaacg taactgatag tcaagaaaag tgtatcatag cccaacttag
     9781 tatcagggtt tgtcaactaa actgtggaag ttgatagata agagttttta acaaccaaaa
     9841 ttctcttgac tatttttgaa atcagacaaa ttttgattgg acacatagtt gaataaatct
     9901 accgtcgtta cggaaggtaa atcgcatgct tttcaattct agaggagaag tcctcgttga
     9961 agtaccatag cccagttctg gattcgacct cctgcccact tcatacgtaa ataaagtaat
    10021 tttaggagtg agactgtgac aaaagttatt tttaagctaa aaacgaatta aaaaacgttg
    10081 ataattaaaa tcattcgttt tagattctat gaatcattat agaacgctgt aaaatcgata
    10141 ctttataatt ataaagaaga tagtttcaaa tttattgttc gaaatttgaa actatctttt
    10201 tacttatgtc acagactccc ttagttgagt tattctttaa tttcgaattt cgagatagct
    10261 agatatattg caagcataca ttcttgataa caatcttgat catattttcc agttctccag
    10321 gattgcttat gaatcaaaga gctaaatctt ttgagaattg cctcaacagc ttcttcattt
    10381 ccagcctttg ctaaaatgaa atcttctttt aaagaattca taaatctacc ttcttcccat
    10441 ggacttataa tctatttctt tgggaaggag gagaacgagc aaagaagcat acaaaattag
    10501 ttattatgca gatattaaac cagcattttt tgaatttttt tgtaaagtcg atgttttaaa
    10561 tttgtcgcac cctgtcttgt aatccccaga acctttccaa tttctttatc agttttatca
    10621 aagataaatt tttcgataag aaaaatttgt tcaggttctt tcaacaaagg aaggattttt
    10681 gctaatttta tatcttcaac aaggagagtc acatttaaaa cattaatcgg ttgtttaaat
    10741 tttatagtat ctttagtaaa caaatatggt gggagttgat caagttgttc atgtttttga
    10801 atttcttctt gttttttata ataagtatta gcggtgttca aaatgacctt ttcaaagaat
    10861 cgaatcaact gggcatttac gcgttcgtcg ttatctgaca tattgcacac ctcctccatt
    10921 aagatttaaa gggaaaagag gcaaaaaaca cgaaaagaag ctaatgcaat tatgctgcgc
    10981 tgcgaataac ttctataagc tcacatgcat tgataggact gctgatgttt attttcaaaa
    11041 atgttttttt agttgataat ggatagttat ctttatactt agaaaagaaa attcatatat
    11101 cttcttaaat atcaaacatg tccttatggc taagaaaatg tcttgagctt tttacgtata
    11161 ataaatcttt tctaaaacct ccttttggat aatattctta ttcgtgtata gattgtattt
    11221 tttatttctt cccccttcat aattaacaac tagaaaagag ttcgaataga cacgtaattg
    11281 aacgaaaatt tttattggat tcccaaattg gaatatttag aaacgaaaaa ttcctattat
    11341 ctgtatagtg cttagataat aacggatcat aataattatt gtctttgaac tagatatatg
    11401 taaatagtat tcatcatgat gaaaaatgat gagttctcgg tgataaaatg tactttttat
    11461 ggtggaatat acatgaaagc gataagtgga tattttataa tgaagtaagt tagcttattt
    11521 tgacagagga atagtatttt agataacgta taaaatacta tttaatgatg tagaaaggaa
    11581 agagagaaac aaaagtgttt tcagataaag aagtcttatt aaagtctctt catgtttgtt
    11641 taaagttacc tatcgtggtt tttaattccg aattcaatat gataaaagag tatcgctcag
    11701 atagaactac ttattttttt tatgattata aaaagttact aactgataat attaaaaaaa
    11761 ctaaagattt ccatttcttt actggtgatt ttaatgagat gtttcttcta tacatgttta
    11821 aaaccagata ttacttattt ggtccattta gagcaaataa tattgataaa gattttttta
    11881 aactaaaaat gaacaatttg aatgttgcaa tggctgatag agaacgactc tataattcat
    11941 tgcaaaattt gactttgtat tctttaggag acattcgaga tattctgatt ttagttcatt
    12001 atttttttac tggaaaaata gaagatctat tccataaacc gttaattgaa tatacagaaa
    12061 atctaagtaa aattatcgag caaatcaaaa tagataatct gttatcacaa aattacgatc
    12121 cagaaatata tctgtttctt tatgaaaata aaatactgga atatgttaaa aatggagata
    12181 ttcgaaattt agaaaacatg gtttttaatc tgagtaatgg aataattcca agtgttagtg
    12241 gagatacgat tcggtctgaa aaaaattatt ctatcattgt ttttgaaaag ttagcacaaa
    12301 cgtctataac attaggaatg gatataatag aagcgtatca aagtagagat gctttgatac
    12361 aagaaaacga attagctgta tcgttacctg aggtcttaaa agtgagggat tctgggattg
    12421 tttattatac taaggaaatc ggaaaaacta agatagagca tctttctccc ttaatttctt
    12481 ctgtcgttca attcatcggc ttaaatatct ataaaagaat tactgtaaag gaaatagcta
    12541 attatttttc tgtaagtgaa actaaattaa gagaaagctt taagaatgaa atgcatatta
    12601 cgatttataa ttattgtcaa tggcggaata aaaatgctgt tttatggcgg tttaaaaatg
    12661 ttgtttttcg gcggtaataa atgcctgtta ttagccgatc gtttctttat ctaagtggta
    12721 atttttcaat cgataagaat taccagtaat tttaaaaact ttagagtgat ggattaatcg
    12781 atctaaaatt gctgctgaag ctgtagtatt atcaaaattt ttcgcccaca tagaaaatgg
    12841 aatgtttgtt gtaataattg tagaagcgcg ttcatatcga cgtgccatca gttgataaaa
    12901 ccaatcagct tgtgtcttac taatcggcgt ataaccaatt tcatctatga tcagtaaagg
    12961 aacattggta aatattttta gtgttctaac taatgttccc ctctgactcg cacgagtcaa
    13021 tttttctata agatcgttaa aaagaataaa ccttgttttg actccttgaa tgcatgcttc
    13081 aatccctata gctgtagcca agtgagtttt tccaacccca ctattgccaa gaaaacaaat
    13141 attcgtacta ctttctagga agtgaaaact tttgagttgc agaatgtcat gcttattaat
    13201 gctaggctga aattcaaaat caaattcttc caatgattta atagttggga agcgcgcaag
    13261 tttaattgca cgcttatcta tcatttcttt ttgataatcc agttgctttt gcgtaagatt
    13321 atacaatgca tctgtcaaag aaattgtctg attcctcacg gaatcaaggt aatgaggata
    13381 atactctttc atttttaata agcctagctc atctaaattg gttaacactt tttggtgatt
    13441 tgttgtcatt attatttcag ctccttaaag ttcatcatat tgagtcaatg ttcgttgtat
    13501 atactcataa atttcttcat catctttgtg tttcatcacg tctgatttta atatttcaaa
    13561 catatcagtt tctttatagt tcaggcgctt accagttaat tcgtgagatt gtacaacctc
    13621 tccttggtag aatatttcga taaatttttc ggttggatca atttctattt caacttcaca
    13681 accaatatat tttgtaggga cagaatattt tgattttcta aaattgacca ttgattcgtt
    13741 ggtgacaata cgaacaatat tgtcttcaaa atagggagtt aacaacgctt cattaactgg
    13801 atgtagtttt cctttttctt cacgttccca tagatcaaca gggcgctgat aagtggattg
    13861 agaaatttct tcataattca aatcgtaaca gagcgtattc actaaactaa ttaagtcaac
    13921 ggcatcatag aattcataat cataaggctt cagtctctca actagcttag ctaaagattc
    13981 tacggagcct ttagtttgag gtcgataact ccggcacgca atagcttcaa aaccagcatc
    14041 tttactgaac tcatagaaac gagtgttaaa aaccacttta cgatattgtg tacgtgcccg
    14101 atcaaccaca gttttcatat tgtcgaacca tatttctttt gggacacctc cggtgtgatt
    14161 aaatgcttca tacaagcatg aaaataaggt gtcctgcttt cgatcccaag ttaatgtaat
    14221 gaatttcaat ttagaaaaat gtagaacgta cagaaagaga ttgaacgtgt aagatttacc
    14281 aaatttatcg tgtaaggtca tatcttcttt ccaatcgact tgagcagctg ttccaggaac
    14341 tgtttcaaca cgaagtgtcg cctttttaat ttctgtgtgt tttttattgc gacaataatc
    14401 cttaacaata gtatagccac cctgataacc aatttttttc aaaaacttga aaatagccat
    14461 agcggaacaa tttaattcta atttctgatc aattatttcc ttgaaaggat ctaacttgga
    14521 gggacgcttg ggcttatctt tgtctttctt tacagagttt tctgagcctt ccagataata
    14581 ctttttgaca gtacgtgggt cgcaattata ctggcgtccc aattcattga aattaggttt
    14641 aatatcgtta atcataaaaa atcgaactcc ttcgtataca tcttttctca atcaattaac
    14701 ctccattcag attgagttaa gaaaagtgta tagaaaaaaa tgtaggaaaa acgacatttt
    14761 tattccgccg ttttcctaca ttttatagcc gccatttaca ttatatctct aagcgaaaaa
    14821 tttctacggc taaaataatg ttgaaatcaa accatactat cagtgaagtt tctttaggtt
    14881 taggattttc agattcttca cacttttcga gagtattcaa aaagtacgca ggtgtatctc
    14941 ctaagcaata tcaattaggt ttagtggaca atttcaacaa cattagctga gaggttttat
    15001 tttattatga gactatatca tttacgtagt ttggaggagt tgaggaaatt ggattttaac
    15061 aaaaaatttt tttgggaaat ttctgatttg agttgtttcg tagaatctga gcgtctaagt
    15121 aatggtaata atagtgtgtt aaatgaatat gacaaagtag taattaataa agtaaaaagt
    15181 ttatgtgaag aagctagaga atctatcagt catattaatg gtagcaattt taagcaaaca
    15241 ttatttaatc tgattaagct agatgctaaa atatcaagtt atttattctt tttaatacat
    15301 gatgaattta tggattatca aaaattggat cagttaattg aaagagaaag ctgggaagat
    15361 tattacgtgg gtctaacttt ttcagaaaag ttaaacaact acaagttaat agattataag
    15421 tatttgagtg aaagcaatta attattcatg tagagtacta gacactttaa atatggacaa
    15481 ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg tcattttttg
    15541 tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta agattctagg
    15601 aatcattcag aaatgctgtg aaaccgatac tttataatta taaagaaggt gatttcaaat
    15661 tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt cagtaatgcg
    15721 ggataaggct ccttattccg ctatttgaat actctgaagt ttaatctaag aaagagtaat
    15781 attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga atacatacat
    15841 atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca
    15901 tgaagaaatg tacttttttt gttgtaatgt gcatgaaatt ctttaagtaa attagttaac
    15961 ataagtttaa taagattcct attctgtgaa taatacgtat cagatttatc ttttattctt
    16021 actaggaaaa taactattaa gggagttgat ttgaataatg attagcaaga ataataagag
    16081 agtatttcta gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa aattaagtgt
    16141 tggggttgca tctgttttgg ttggtgttgg tcttgtcttg ggaacaactg aactagttaa
    16201 agcacaagat gaaatttctc caagcacccc acttgaaaca gcgataagct ccgtacaagt
    16261 aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat cctggttata cgaaaaatta
    16321 taacttttcg gatttacaat ttagtcctca agaactaact ggagacacac taaaaggcaa
    16381 tacaattgga tttgaagttt acggaaaaca taatattgct gcttctaccg ataattggga
    16441 aatccgcctc caattggatg agcgattagc caagtacgtt gaaaacattc aagtagattc
    16501 taaaaaggtt gcggggatta gttacagaag aacttttgtc cgaatcaatg attcattagg
    16561 tagacccacg aacatttgga aagttaatta tattcgtgca agcgatggat tatttgctgg
    16621 ggcagaaaca actgatacac aaactgctcc taacggggta attacatttg aaaagaatct
    16681 agatgagatt tttcaagaaa ttggtgaaga taaccttaag agtgatcgtt tgatgtatcg
    16741 aatctatctg gtaagtcatc aagacaatga taaaattgta cctggtatag aaagtactgg
    16801 ttactttata acagatcaag ataatttcta taataagtta gacgtatctg aaaataatcc
    16861 tgatcaattt aaacacggat cggtaagtac caaatacgag aaaccaaatg ttcaaacgaa
    16921 agatggatcg gggtctactg gtgctaatgg cgctataatc ttggatcata agttaacaaa
    16981 agagaaaaat ttctcttact cggtttctgc aacgggaacg ccttggtatg ctaactacaa
    17041 aattgatgaa cgtttagttc cgtatgtagc gggtatacaa atgcatatgg ttcaggctga
    17101 tcgagtagta tatgacgttt catttgaatc aggaacaaaa gtagctgatt tagcaatcga
    17161 gcgacgggaa ggtcatgaaa attatggtgt aggttcaata actaataatg atttgactaa
    17221 acttatcgat ttttctaatg caagtccacg tccagttgtt attcgttatg ttcttcaatt
    17281 gactgaacca ttagatgaaa ttttagcaga aatgaaaaca gctgcacaag ttgaaggaaa
    17341 tgaaccgttt ggtgaagact tcatctttga ttcttggttg tcggatacga ataaaaaatt
    17401 aatccagaac acttatggaa caggttatta ttatttacaa gatattgatg gtgatggaaa
    17461 ccctgacgat aaagaagaga gtggagacac gaatccatat atcgggaaac ctgaattaga
    17521 agaagtatat gatgttgata caacagttaa ggggaaagta ttcatccacg agttagcggg
    17581 aacaggtcac aaagctcaac ttgttgataa agaaggtact gtattagcag aaaaaactat
    17641 tgctccaaat gaaaaagatg gagctccaat ttcggatact gtagaatttg aatttacggg
    17701 tgtggattca aataaattaa ttgcgaaaga tgaattaaaa gttcaaatcg tttctccagg
    17761 ttttgataaa ccagaagaag gttcaaccgt tatcaaggaa tcaccaaaag cggttgataa
    17821 acaaaccgtg gtagttggat ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa
    17881 cttacctgaa gatgcagagt attcatggaa aacagagcct gatacttcta acgttgctga
    17941 tagtacgaaa ggtattgtaa ctgttaaaat cggaaatcga acttttgacg tagatgtaga
    18001 gtttgctgta aaagcttctc aagctatgga aaatgatgca acatacgtac ctataacaac
    18061 aaccccagaa acgacaattc aaagtggtaa acctacattt gataaaccag atgttcctct
    18121 agctaacgat gccttttcaa ttttagatgt ttataataag gacttcggca atgcaagtgt
    18181 tgacgcaaat actggtattg ttacattcac tccagccaaa ggggtaggag aaacggaacc
    18241 tattactgga acaattccta tcaaaattgt ttaccaagat ggttctgtag gtacgaccga
    18301 tttggcagta actgtaagta aagatattt
//
LOCUS       DAFEZB010000055        17303 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFEZB010000055
VERSION     DAFEZB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.055"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(301..615)
                     /gene="trxA"
                     /locus_tag="LP859_001990"
     CDS             complement(301..615)
                     /gene="trxA"
                     /locus_tag="LP859_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL3029922.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(702..3062)
                     /locus_tag="LP859_001991"
     CDS             complement(702..3062)
                     /locus_tag="LP859_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL3029923.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(3177..3725)
                     /locus_tag="LP859_001992"
     CDS             complement(3177..3725)
                     /locus_tag="LP859_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL3029924.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(3783..4220)
                     /gene="zapA"
                     /locus_tag="LP859_001993"
     CDS             complement(3783..4220)
                     /gene="zapA"
                     /locus_tag="LP859_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL3029925.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4422..5339
                     /locus_tag="LP859_001994"
     CDS             4422..5339
                     /locus_tag="LP859_001994"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL3029926.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(5717..6628)
                     /locus_tag="LP859_001995"
     CDS             complement(5717..6628)
                     /locus_tag="LP859_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3029927.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(7029..7607)
                     /locus_tag="LP859_001996"
     CDS             complement(7029..7607)
                     /locus_tag="LP859_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3029928.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(7627..8295)
                     /locus_tag="LP859_001997"
     CDS             complement(7627..8295)
                     /locus_tag="LP859_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3029929.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(8310..9380)
                     /locus_tag="LP859_001998"
     CDS             complement(8310..9380)
                     /locus_tag="LP859_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3029930.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(9551..10915)
                     /locus_tag="LP859_001999"
     CDS             complement(9551..10915)
                     /locus_tag="LP859_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3029931.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(10912..12471)
                     /locus_tag="LP859_002000"
     CDS             complement(10912..12471)
                     /locus_tag="LP859_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3029932.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(12527..15007)
                     /locus_tag="LP859_002001"
     CDS             complement(12527..15007)
                     /locus_tag="LP859_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029933.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(14991..15326)
                     /locus_tag="LP859_002002"
     CDS             complement(14991..15326)
                     /locus_tag="LP859_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029934.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(15340..16374)
                     /locus_tag="LP859_002003"
     CDS             complement(15340..16374)
                     /locus_tag="LP859_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL3029935.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(16464..17144)
                     /locus_tag="LP859_002004"
     CDS             complement(16464..17144)
                     /locus_tag="LP859_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL3029936.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         4958 a         3597 c         2980 g         5768 t
ORIGIN      
        1 cgatttttta atattttggc tttgccgaca taaatgatcg ttccattttt atctttcatc
       61 aagtaacagc cgggctgatc aggaagtaaa gctaatttgt ttttgattcg ttcgttcatc
      121 tggtactccc cttcatattc aattaaagtt attatagcat gtctgtcaaa aaaacaatcc
      181 agcctgacaa cgtaattata ctcaaaaaat attaatttga ataaccttaa ctcaaccgca
      241 aaaaaaccga ggacggaact ggattcagtc ccaccctcag cttatttttg ctgtaagttt
      301 ttataaatat tgatcgatca tttgacgtag ttgttctttt gaatgaacac caacagcttt
      361 ttctactact tgaccatctt ttttcaacat taatgttgga atgctcatga taccgaattg
      421 ttgaggagtt tctggatttt catccacatc cattttagca attctgaatt cttcttcatc
      481 atattcttgt tccaactgat ccaaaatcgg tgcttgcata cgacatggac cacaccaagt
      541 tgcccaaaaa tcaatcaata caagtccttt atctgtttct tcattaaatg tcttatctgt
      601 aattacttgt gacatatcaa agacctccat ttttaaaatc tatgactagt atagcatttc
      661 tatacttgga tagcgaacca tctgcttacc tttagtaagc tttacttaaa tttaacaatt
      721 gtcgcaccat ttccgccttg gtttgccggt gcaaactcaa agcttttgac gctccgatgg
      781 tttttaagat aatcagtaat tcccttgcga agtgcgcctg ttccttttcc gtggacaata
      841 gtcacttgag gataaccagc tagaatggca gaatccaaat attgatctac ctcacttagc
      901 gcttcttcgt atcgttttcc tcgaaggtcc aattgattcg gcacgtgact actagattca
      961 gatcgtacag catggataac tcgttgtgtc ggctcttttt gaggagcaac aggcgtcagc
     1021 tcgtcttcgg acacattcat ttttaatata ccgatttcta cttgccactg attcccgttt
     1081 ttacgtaaaa gcgtccctct ttggccgtaa gtcgtaacca acacttcgtc tcccggtttc
     1141 aacgtttttt gttctttcgc cttcttcaat actttatttt tcttcaaagt ttcttcttga
     1201 tgtagatttg ctaattgggt ctttgcatcg atcaactgat gctctttcac atttccttgc
     1261 ccaatctgct gttgcatttt acgaatatca gcaatgattt tttctgcttt ttcttccgcc
     1321 tctgacacga cttcatttgc ttttttcttt gctttttcca tttctttttc acgttcttca
     1381 aagaaatagc tgtaagcttc tttcaaatca tcgtgaagtt cttgtgcctc agaaacaaaa
     1441 tgacgcattt ccagatattc ggtctctgcc attttccggc gattctctaa atctgtgatc
     1501 atttcgttta aatcttggct ctcatcattc atcaactgct tcgcttcatc aataacttct
     1561 gtatcaagac ctaatcggct agagatttca aacgcattgc ttcgtccagg aacgccaatc
     1621 agcaaacgat acgttggact taatgtatcc acatcaaact ccatgctggc attaatcgta
     1681 tttgcccgat tatatccata aactttcaat tcaggataat gcgttgttgc catcacataa
     1741 gcagactttt taccaagatc atctaagatt gcgattgcta gagcagcacc ttcttgcgga
     1801 tctgttcctg caccgagctc gtcaaataat accagacttt tttcattgac ttttgataaa
     1861 atatctacag tgttcgtcat atgagaagaa aaggtcgata gactttgctc aatcgactgt
     1921 tcgtctccta tatccgcaaa tacttcctca aaaataccga tttgactttc ttcacctgca
     1981 ggaatcggta atcctgcttg ccccatcagt tgtaacagac ctaaagtctt caacgtgatc
     2041 gttttcccac ctgtgttagg tcctgtaatg acgatggctt gatagtcttt tccgattgtg
     2101 atgtcattag aaactacttt ttcctgatcg atcagtggat gtcttgcttg tttcaagacg
     2161 acatgattgt cttcactgat tcccggcacg atggctttta cttctttacc aaatcgtgct
     2221 tttgcattca tcagatccat tttaccaatc acataagtat tgtggatgat ttcttgacga
     2281 tggggaacaa gttcagcaga aagttcactt aaaatacgtt gaatctcatt tcgttcagcg
     2341 atttggtatt gacgcaaacg attattcaaa tccaccactt gttttggttc aataaataaa
     2401 gtctgacctg aagcactttg atcgtggact acaccgccaa atacaccgcg gtattcttgt
     2461 ttgactggga taacgtagcg gtcatttcgc atggtgatga tcgcatcact taaatacttt
     2521 gcatttttcc cacggacgat cccatctaac tgttcacgaa cagcttgttc acttcgccga
     2581 atgttctgtc ttatgctctt taactcagga gaagcgtcat cagtcacgcg tccgtcttca
     2641 tcgattgctt cctttaaacg gcggctcaaa acagggatcg tgactaactg atctgcccac
     2701 atatacaaac gtgcaaattc gatttcactg tcagaaagat catcaatgaa acgattcaat
     2761 tcgcttgtcg tagacaatac tctggaaact tgtgcaagct ccactccatt caagtctgct
     2821 ccgatctcaa tccgtttcat atggggacgg atattttcaa ttttaggtat agggatcccg
     2881 cctcgcagac gttgcacttt caatccatcc tcagtttctt gaagccagtt tgtgatcgtt
     2941 tgtttctcac tcactggtac caattctgcc agttcttctt ttccttgtgc agtcactaca
     3001 aattgtctga ccatctgttt gactttttca aattccaatg tttcaaggat tcggttattc
     3061 attttatcac cttatctttc actatgaaaa aagagctgtg ccggacatac gctacgcagc
     3121 cttcttaaat aaactactct gtacaaaaaa cgaaatattt tccagcacta ttttccttaa
     3181 ttcaaaatat tcgttaccca aagctcatac atcttgttgg taagaaatgg cgttctctca
     3241 acgataaaac gagcgaaact atgctcgccg aattgatttt ggaccacatc taatggaaca
     3301 aaggcaagta actgcaaaat caaaaatata gtaacgtaag ccatgattag tgacaggatg
     3361 cctcctgcaa tccagtctac ctgtctcaag atcggcacat acgtcaatcc atggacaaaa
     3421 acccccacaa atcgggtaag cagccagcca attagcaaaa taatcagaaa agcagttcct
     3481 gcataaaaag cttcatctag gtgaaaggca aatacctgat caaaaaatgc taactttgat
     3541 gttggggtga cagctgggta tggaatgtat aattccaaat gacttgctaa tggtttgtaa
     3601 aaatgctggg cagcaataaa tgacaataca tatccaatcg cgaatatgat ttgcaaagca
     3661 aacccgcgac gtgctccaga aaaaaacgca attagcaata aaaataaaat cagcaaactc
     3721 agcatgctac gttcctttct cttctaactt ttttaaattt ttcttatttt gataaaagga
     3781 tcttaattag aagatttttg tttaatttgt ccttttcgtt tctcgtttaa aatctgttgt
     3841 gcttccacat gatttttaat ggtatgatct gcttgaccat tttctttcaa tacttcccga
     3901 gcttcgttct cgattgcttc aatgcgtttc acacgatttt caagctctgt aaatttgatc
     3961 atttttttct ttaattctgc tgtttcttct tccagctcca atattctttc ttgtttcttc
     4021 aattgatccg aaagcgcatt gacagccatc aagattgctg cttgttcatt atccatttgt
     4081 ggtgataatt gtttcaattc tgctaactgt tcattgatca gcttcgttac gatatccata
     4141 tgatgcttgg tttcccgtcc gataatagta tatgtttggt tggcaatcac cgctttgtag
     4201 cgtgtttttt cgtgcgccat acgaatcatt tcctcctatt tgatgaaagt tttatttagt
     4261 gtaccatgaa gttggttctt tcaccgttct ccacaaatat ttttcacata cttaactatt
     4321 atatgataaa ctttaaaaaa atgctatgtc tcgcaatctc ttagaaaacg aaaagtcgtg
     4381 agttgcggta tagttttatg tataaaaacg aggagaattt tatgagcaac gtcattatca
     4441 aagcaacaaa cgaccaactt agtcaaatga aagcatacta tcgctcttat ttgttaaata
     4501 aacagattcc ttatactagc tttgctgcaa aaaaagacgg gacgaccatt accgcttata
     4561 catccggaaa agtgatgttt caaggtacga atgcagcaat agaagctgat aaatgggggg
     4621 aggcacagga taaacaagcc aagccagcaa aaacagcctc ttctttgcca aaaaatatca
     4681 gtcaattatc cgttttaggt agtgatgagg tcggaaatgg cagctatttc ggtccgctaa
     4741 cagtatgtgc tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa cttggtgtta
     4801 gagattcaaa agagttaaaa gatagccaaa tcatccagct tgctgacatc ttaaaaaaaa
     4861 ccatccccta taaactgctt gtcttatctc cagaaaaata caatgagatc caaccaaaat
     4921 acaatgccgt tcggatgaaa gtcgctttgc acaatcaagc gatccatttg ctgctaaaaa
     4981 aaatcgagcc agaaaaacca gaagcgattt tgatcgatca gtttacgccg gaagcaaatt
     5041 ttaaaaaata tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac ttcgttacta
     5101 aaggagaaca atatcattta gctgttgctg ctgcttctat catcagtcgt gccagctttt
     5161 tggaagagtt agacaaagca tcagctgaag ctggaatcac ccttccgtca ggagctggaa
     5221 cgaaatctga ccaagtagcg gcaaaactat tggagaaagg cggcatgcct atgcttgaaa
     5281 aatatgccaa acttcatttt gccaatacag aaaaagcttt gaagctacta aaaaaataat
     5341 ggaactgtga caaaagtcat gtcacagtcc cttgttccga ataaacggtg ttcaatcatc
     5401 tgatcctcgg tattagctca acaaacggca aaatatgagg aactattttt ccgtttgttt
     5461 ctctaatact cagaacaaaa cgttgatttc acaacctttt tcttaaacgg tgttctcatg
     5521 ccaactcctc ggtcacaagt cacaatagtc atcaaacatg agaagctgtt ttttgaaatt
     5581 gctccttgtg actcagagtt gaacggcagg ctcacgacct cttccttaaa cggtgtttaa
     5641 aagcttgcat ctgcgataat aagcccggcc aaacgaaaaa tatgaggagc tatttttgtt
     5701 tggctgggct tatttattac tgaaaataag tggtcaatgc ttgtgtcaaa cttgcagcaa
     5761 tctgctgctg ataactgtct gttactaatt cctttaaatc actatcatta ttcatataac
     5821 ccatctcgat taaaatagaa ggttgaagat tttctcgaag aacttgatag ttcccagtgg
     5881 agattcctct tgaatccaga ggcagatcag tgaaggagtc agcaattgtc tgtgctaatg
     5941 tttcctcttg tccatagtaa taatatgtcg tgattccagt tgcttcattc gcatattccg
     6001 tagagtctgc gtgtaaactg ataaagacat ctgccttttt ttcttcactg tagtcgcata
     6061 tatctccaag ctgtactaat tcatctgtag tatgggtcaa ataaaccgtt gctccagcat
     6121 cctctagcgc cttttttact tttatggctg tacttaatgt tatatctttt tcttctgttt
     6181 gttcatcatt gcttaatgcc ccagtatcat ttccgccatg cccaggatct aatacaatca
     6241 ctgcatcaga tagctcttcc ggttttgttt gtatattctt tgtgactttg gttccatctt
     6301 cgagggctaa atatccagta tagccatcat ttgtcttgat ctgataagca ttttttgttt
     6361 cagaaagaaa ctccgccagc tctccgccgt ttatagttgc aagtggggta gttagcgagt
     6421 catctgtata tagtgtacgg tcattttttc cgattttgat taatgtctct tgttctgtat
     6481 catcaaatga ttcctttgtc tttgtaaccg tactgttcgt actgctgacc gtttgtccaa
     6541 ctatcttttt ttgaccaaaa acatctattt gaaaacagat aataatgata atgattgcaa
     6601 atacaaaagc tccaattatt tttttcattc ttgactctca cctcgtagat ccagactaag
     6661 ggccaaaact taaaatcagc ttaagtaagc aaaaaagggg ctgtaaaaga tataaccgta
     6721 gaaaataaca ttactgctaa aatgattttt ttcatttttg gcatatcctg aaatctcctt
     6781 ttgactattc gtggtgattc atctacccaa taaatagttt ccttcattct acaaacaatt
     6841 aatttcttcg ttattaaatt aaaacacttc ctggaacacc gacataataa ataaaaatat
     6901 accctgataa tcatgagaga ataacaaatt tgtatttatg aaaaactacc tatataaaga
     6961 agcaagatat aacgatcttc gtcatatctt gcttcttcac ctaactattt tctccctata
     7021 taacagcttt acgcttctcc taatacacca ttcaaatata aatcgatcaa ttgcgccaca
     7081 tttagcggtt cttccacagg tcttttcgac cgagtgatgt aaggtgctaa cactaagaaa
     7141 aaatgccttg ctgcgatttc ttttgaaatc ccatttctta gcacagattg gtttgctgaa
     7201 aagatttctt caaaaggttc acgataattt ttcttccata gaaagaaaag atgatctctt
     7261 gtttcctttg aaagattgct ttcaagatcg tgaagcatca gaaacaaatc gaccagatgt
     7321 gtctcaatca aaaactgcgc catttttgtc aaggttactt gaaatcccgt ttcgcttgtt
     7381 tgaacgattt ctctcaaagc ttttccaaca tcttttaagt ggttttctaa aactgccgta
     7441 tatagttttt ctttatcacc gaaataatga tagagattgg gctgggtgat cccaactttt
     7501 tgtgcgattt ctcttgttga agtgccgaga tatcccttgg acataaacaa gtcagtcgct
     7561 tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat ggaccatttt tccacctgct
     7621 ttttggttat tctttttctt tcaatacacc atcttccatc ataaatattc tatcgcattt
     7681 gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt cttttgattc
     7741 ttttgccaag atatcgacta cttctcgtgc cttttctgaa tccaaactag cagttggttc
     7801 atctgctaga ataatggacg gatcattgta caatgcacga gcgattgcga ccctttgccg
     7861 ttctccacca gatagttctt ctggatactt gttgatcaac ttatccacgc caagctgctc
     7921 aaataattcg tttgcttctt tttgacgatg ttcatgttta atcttatcta ctaataataa
     7981 ctgctttttt actgtcagaa aaggaaccag attggaagct tgcaaaataa agccaatttc
     8041 tttaaaacgt agtttaacgc gttcgctttc tttttgatta ctgaattctt gatcattgat
     8101 aaatacagag ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa aagtactttt
     8161 acctgaacca gaaggaccga tgattgcaac aaattcccct ttatcgatac tcatggttgt
     8221 ttctttcaat gctttaacta cttggtcgcc atcttggaaa cttttttcta cttctttaaa
     8281 ttctattgct ttcatgttct tcctccttct tatccaattg ccttcaacgg atcgattttt
     8341 acgattgtcc gaacagaaaa tagtgcgcct aacacagcta ctactaacat caacaagcta
     8401 atgacgccaa aaaacagcca attgctttgg aatggaaccg catcaggtag aacaagggat
     8461 gttcccactg ttcctaatag accaagaaga ataccaacaa atgataaaat gaatgtttgt
     8521 gccactacag aacgcgcaat aaatcctcct gtgatccctt gtgctttcat tacaccaaag
     8581 atattaatct tttgcatcgt cagtacataa atgaaaatcc cgatcacaat agccgcaata
     8641 acgatcaaaa atccgatcat aaatccaaaa gtcaatactt gcgcattata tccaggtaat
     8701 tcattgatga atttagaaat tttgatctgt tctaaatctt caggtaaatt actgatttta
     8761 cctctgacaa ctattgcgtt aatccgtgca ttttcacttg tatcttcttt ttcgaagcgg
     8821 atttcttggt aagcattgat ggttgtatag agaactggtg agacattgaa tttggcatga
     8881 tccgtaaagc cagttagttt aaacgtcttg tcactaccag ataacttaac tgtatctcct
     8941 atggctagac catattcttc tttcaaacta atatcgccta ctgcctcatc atcgttatca
     9001 aaagcttttc cttcaacaag attgggcatg atgaattgat ttttatcgat accgaagaag
     9061 ctaacattga tttttccttc ttctgtactg tctttactag tcacgactcc aggtgtttgg
     9121 gcaagatacg ccactttccc accttcgact tcttctgcta tcttcttagt aatcatggac
     9181 atccctagat ttgaatttgc atctttcgaa agcacaatcg aatctgcctc ccacttgtcg
     9241 actgctgttc gattatcttg agctaagcca taagctaacc cagtcaaaaa aaataccagg
     9301 taggcgatca aaaacatcac ccctgcaact aacgcataac gtaactttga atgcataatt
     9361 tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat aatcatttta tacaacaatt
     9421 ttatcacttg ataaattttt ggcgaatatt atgctcaaaa aattcttccg ctatttcatt
     9481 atatgaattt tttattctcg attgattaat atttattata tccccacaca ataaaagacc
     9541 gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta gctagtactt
     9601 taaaagctaa ctcattgagg tagtgaaaac gagaggtgta ttcagacaga aatccaaaat
     9661 cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca agcaggttca
     9721 aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct aatgtatccg
     9781 gatggtagat cagctttgca tttactgttt tgttttcttc gtcgtattgt ggaaatagat
     9841 caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca tcttctgtca
     9901 gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt tgtctgaggt
     9961 cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata tgtacagctc
    10021 gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact gggacgatat
    10081 ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact atcacagtct
    10141 catcctcata caaagccact ttttctttta acaatgtcgt attaggacga gtttgggcag
    10201 aaaaaatgac catatctgcc tttattgttt gagacagaag ttgtacggat actactttct
    10261 cttctgattt tttccaatcg atcaagaatt catttggata aaaatttatt tgcttagttt
    10321 gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat aaaacattcg
    10381 gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct gctaattcca
    10441 atccaatgta accagcaccg aagatcacta cttcttttgc ttcttttaaa gcggcaagta
    10501 cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca atttgtttat
    10561 acaaggtgac aggagaggaa cctgtcgcta cgattaattt atcataggtt atggtctcgt
    10621 tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt tcagttactc
    10681 tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta tgggcaactt
    10741 cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa acatagccta
    10801 gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga agttttctta
    10861 gatttagggc agctgtgatg ccttcatggg aagcaccaat gatcacgatt ttcatgtttt
    10921 attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt tcaaataggg
    10981 gaaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact tctgtgacgg
    11041 gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct cgtattcgac
    11101 taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca cgatatccgc
    11161 cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta actgcttgat
    11221 cagcgtccgt aaaatagatg catagcgagc aaataattca aagcgggcat tttcctcgtc
    11281 tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac ataaaaccag
    11341 gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc caaaaatatt
    11401 catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca acgcaatctt
    11461 ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg attgtttaaa
    11521 cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca aaaactcctg
    11581 gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat aaaaaaccaa
    11641 cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca aaaatgtatg
    11701 gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc tataatctgg
    11761 tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata tttcaaattt
    11821 caatcgtcct tcttctgtaa tagcaaaact aaactctttt tgcagcagat ctaaaaaagt
    11881 gtttcctgat aaatcactgt attctttttt gttttcaaga tagggacgtc caaaccaaaa
    11941 taattggaaa taaaaataac gaatttgact ttctggaccg gtcagccgcc cgctttttat
    12001 tttcagatga aactcattta aagaatcatt taattccttc actctgcgag acaaagtagc
    12061 cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat tttccaacag
    12121 ataaagaagt atctgatatt ttgtagaata gcgcaagtaa caatgataat aaacatcgac
    12181 tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa caatttccaa
    12241 ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca aggtagcccg
    12301 atcaactgat aagcgggtaa gtacgttatt aacggaaacc tctccttttt cctctaataa
    12361 aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga aaagttccaa
    12421 gtccactcct tcaagcattt ctgattttaa cagcagaagt ttccttctca tccttgaaaa
    12481 agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag ctggttggcc
    12541 cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga gcattcgtac
    12601 tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga acctctcctt
    12661 tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc aattgctttg
    12721 atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga tcattcttaa
    12781 ataaagaagt caatgtacct gtcagttgcc aaggagacgt gcttggaagc cgagtatccc
    12841 gcacatcaaa agaaaaatcg ccttttgatt cataataact tgtcttcaac ggtttgggga
    12901 gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct tgtttgggga
    12961 caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca ccatctgcta
    13021 atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat tgtaaagaag
    13081 tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct attttgctca
    13141 atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata gttgtaccat
    13201 ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc gtgatttttt
    13261 tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct tgaatcgtat
    13321 ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg ttttttagct
    13381 gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga gtggtgattc
    13441 cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta agtacatcgt
    13501 ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt gtagaagtag
    13561 ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct gtccatcctg
    13621 tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc ttttttagtt
    13681 cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc tttccccact
    13741 gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac tgctccaata
    13801 cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg tacacgatat
    13861 tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt gctccttgtg
    13921 taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat ggcaactgat
    13981 caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag cctggaaggt
    14041 tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat tttcctgttg
    14101 aatcacttgg tgagatgtaa ctattccacg tatttatctc tgcacttgca gaaaatgcat
    14161 atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct gcagctggag
    14221 ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa aaatcaacag
    14281 aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa attttgtttg
    14341 gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct gtaacatcaa
    14401 attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca ataatatctg
    14461 tcaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg cttcctgctg
    14521 ataataaact tccgctagta accttgatgg gggcaccggc aatatacaag taggcgttgt
    14581 tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa ttgatgatcg
    14641 ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt gttagtttca
    14701 catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt gaaccatcgt
    14761 tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg acaagcttcc
    14821 cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac gtgtatggat
    14881 cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa aagataaata
    14941 tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat ttattttgta
    15001 gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc aagaagccat
    15061 aattctatac caatgatcaa ataaagaaaa cggatctttt gttcattcgt ctccgggaat
    15121 acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt tgatgcgcca
    15181 ttgactgaac cacttggatg cggaattgga cttagatcat aaaatgtaat cgtacctgaa
    15241 cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact tgcaataatc
    15301 cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt ctcctttttt
    15361 ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa ctgcagcaac
    15421 caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg aattttcatt
    15481 tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc attcattact
    15541 gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt tatcaccagc
    15601 taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaatgcat tagacgtatt
    15661 ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat tgctgataat
    15721 cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga cctcgtccaa
    15781 atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt gtaaaacgac
    15841 tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat tttctgtttc
    15901 agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa agctttttgc
    15961 tgggattttc acttctatag gtacagttgt ttctgcatgt gctgatacat tgatttcctt
    16021 tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta acgttgggtt
    16081 tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga cagagacctc
    16141 aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct gattttgttc
    16201 cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct ctggaaatac
    16261 cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg gaaaaatgaa
    16321 aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat tcattgtcca
    16381 ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat tttcaccgca
    16441 gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt ccaagtcaaa
    16501 tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt tgctggatca
    16561 taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat tggtgtcgct
    16621 gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct
    16681 gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa
    16741 ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca
    16801 cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact
    16861 tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc gggaactaca
    16921 tccagtgtta aaggacctgt ctggccagtt gctggatttt ccggatctgc tggctctact
    16981 ggttttgaag gatcagtagg gttaagaggc tccacggtat ttgttggttc agtaaaggta
    17041 atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga
    17101 agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt
    17161 ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc
    17221 ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa tcgtttacat
    17281 gaatattctt ccttttttat taa
//
LOCUS       DAFEZB010000056        17173 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFEZB010000056
VERSION     DAFEZB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.056"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..288
                     /locus_tag="LP859_002005"
     CDS             <1..288
                     /locus_tag="LP859_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL3029937.1"
                     /translation="TDMPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQI
                     LYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            301..687
                     /locus_tag="LP859_002006"
     CDS             301..687
                     /locus_tag="LP859_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029938.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1238..1462
                     /locus_tag="LP859_002007"
     CDS             1238..1462
                     /locus_tag="LP859_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029939.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1480..1908
                     /locus_tag="LP859_002008"
     CDS             1480..1908
                     /locus_tag="LP859_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029940.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1886..3304
                     /gene="terL"
                     /locus_tag="LP859_002009"
     CDS             1886..3304
                     /gene="terL"
                     /locus_tag="LP859_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL3029941.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3316..4851
                     /locus_tag="LP859_002010"
     CDS             3316..4851
                     /locus_tag="LP859_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL3029942.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4931..5809
                     /locus_tag="LP859_002011"
     CDS             4931..5809
                     /locus_tag="LP859_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL3029943.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5811..6128
                     /locus_tag="LP859_002012"
     CDS             5811..6128
                     /locus_tag="LP859_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029944.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6255..6878
                     /locus_tag="LP859_002013"
     CDS             6255..6878
                     /locus_tag="LP859_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL3029945.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6893..7783
                     /locus_tag="LP859_002014"
     CDS             6893..7783
                     /locus_tag="LP859_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL3029946.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7806..8021
                     /locus_tag="LP859_002015"
     CDS             7806..8021
                     /locus_tag="LP859_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029947.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            8033..8374
                     /locus_tag="LP859_002016"
     CDS             8033..8374
                     /locus_tag="LP859_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029948.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8374..8745
                     /locus_tag="LP859_002017"
     CDS             8374..8745
                     /locus_tag="LP859_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029949.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8738..9133
                     /locus_tag="LP859_002018"
     CDS             8738..9133
                     /locus_tag="LP859_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL3029950.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            9135..9512
                     /locus_tag="LP859_002019"
     CDS             9135..9512
                     /locus_tag="LP859_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029951.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9513..10121
                     /locus_tag="LP859_002020"
     CDS             9513..10121
                     /locus_tag="LP859_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3029952.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            10121..10462
                     /locus_tag="LP859_002021"
     CDS             10121..10462
                     /locus_tag="LP859_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029953.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10704..13493
                     /locus_tag="LP859_002022"
     CDS             10704..13493
                     /locus_tag="LP859_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL3029954.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            13483..14223
                     /locus_tag="LP859_002023"
     CDS             13483..14223
                     /locus_tag="LP859_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3029955.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            14220..16931
                     /locus_tag="LP859_002024"
     CDS             14220..16931
                     /locus_tag="LP859_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL3029956.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            16944..>17173
                     /locus_tag="LP859_002025"
     CDS             16944..>17173
                     /locus_tag="LP859_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBL3029957.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT         6114 a         2451 c         3611 g         4997 t
ORIGIN      
        1 acggatatgc cgaaggcacc gaagcacggc aataaggcag aagacgcgat cattcaaatg
       61 atggatatag aagcggagag agacgcgatt ctagtggctt tgatggctct tagtctgatt
      121 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacaggatt
      181 agccgtgaag tgggttattc agaaagaagc atacaaagga tgaagtcgga agctctaata
      241 gagtttgcag aagcgtataa acacggaaga ataattgctt ataaataggg ggaccatata
      301 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
      361 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
      421 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
      481 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
      541 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
      601 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
      661 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
      721 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
      781 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
      841 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
      901 atctatcatc cgttgctgtc tattgttaca tacagatcgc ttaggcggtc tttttatttt
      961 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
     1021 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
     1081 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
     1141 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
     1201 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
     1261 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
     1321 tcaaatgaag gcgaagtatt cgattgtaga aatgatcaga gaggcaacgc cagtagagat
     1381 tgattattgc aagctagtct attgtgggtg cggccgttgg aaagaggatc atgtacaaaa
     1441 aaatatcagc aagtacatat agagaggcgg tgattaagga tggaaaaaag cgatacgaaa
     1501 gcgaacagat acaaacctac tgctgcagag aaaaaactct tggaagtatt gataaatcca
     1561 gataacttag gcaaaccggt gcaagagttg tgttcactgg cgaaaatagg taggaccaaa
     1621 tattacgacg ccatgagcaa agatggcttt gttgatctag ttaacgaaac aacaatggat
     1681 ctgatcaaag gaaaagcttc tgatgtcctt aatgccactt acaagtatgc attgactgaa
     1741 aaaggtcatc aggataggaa aatgattctg actattgctg gaatctatgc agacaaacaa
     1801 gaactcaaac attctggcgg cgtcgatatc cgaaaacagt atgagaaaat gtccgatgaa
     1861 gaattagaag agttggtgaa acggtatgaa aaaatcaacg actcttaatc gtgttgagta
     1921 tgccgttgtt caaagagagc tagccattcg aaaagctagg aaaagttttt ggctattctg
     1981 ccagcttctt tatccagagt tttacaaaga tgatcggttg tatttgaaag atttatgtca
     2041 tacgctacaa gatttttacg aagataaaat cgacaaaaaa attttggtta ttaacatgcc
     2101 gcctcgacat gggaagactt ttacagcgcg gctattcgtt ttatggatgt ttggacaaaa
     2161 tccaaagacg aaaattatta ccggatctta taaccaaatc ctgtcatctt tatttgcgca
     2221 acaaacacgt gatggaatca tggttgaaaa cgaaaatgtt aaggctgaat actttaaaga
     2281 tatttttcca gaggtgcaaa tcaaatatgg agatgctgca aagggtttct ggtctttaga
     2341 tggttcagaa gaaaagaatt atttagcgac ttctcctggt ggaacatcca ctggtattgg
     2401 cgcgaattat gtaattgttg atgacatcat taaaaccgca gaagaagcct ataacgagcg
     2461 tgttttagat tctcactggg aatggtataa caacacgttg gcccaacgaa tggaacggcc
     2521 acgtaaacag atattgatta tgacacgttg gtcaagtaat gatttagccg gaaaaatgtt
     2581 gacgagacga aaaaataatg ttcatcagat ctgttataaa gcagtacaag aagacggctc
     2641 gatgttgtgc aacgagatta tgacccacga tgagtactta gatgtggtac aagaaatgaa
     2701 tgtagatatt gcagaagcca actatcagca agaaccaatt gaccaaaaag gcaggctata
     2761 tcagaagttt cttacttatg atacgttgcc tgataacatt atcaaaatat ggaactacac
     2821 cgacaccgcc gacaagggtg ccgattactt tgcgtctcct gtttttgctg agacgtctga
     2881 tcatgatgct tatattatcg atgtcatgta tacaaaagag ccgatggaaa caactgaaat
     2941 agcccacgct aatatgatta ttcgcaatcg agttaataag gtacggattg aaggaaacaa
     3001 tggcgggcgt ggattcagaa gaaattcaga aagaattaca aaagagcgag gctatcgagg
     3061 tgcagtattt gaagactttc atcaatcgga taataaactg tctagaatac tggcgaatag
     3121 tgcttgggtg gaaaatcatg tttactatcc agatgattgg gcgacacgtt ggccggactt
     3181 ttatgaggct atggttagtt atcaacgtga aggtaagaac gctcatgatg atgctccgga
     3241 tgcagttaca ggaattgcag aaacgattat agaagcagat gacttcgagt ttattgctta
     3301 ttaggaggtg ggtctttgtt ttattctgga atcacattga aaaaatacaa acgtttaaga
     3361 acgaaatatt caacgcaaat cgaagaagga ttattcgatc ctaacgcttt tatggaagat
     3421 atgaaaccct ttttcgatga tcgagaaaga aagtatcttg cttatactag cgagaaaaac
     3481 gagatcgaca gtcgtgagaa accaaaaaca aaaatcatta aggtcaataa caagattcat
     3541 gcgggaatgt attcagtggt cgttgatcaa gcagttaacc atttcacagg aataccaatt
     3601 aaatgggatt atgatgtttc ggaacagaaa aggacattca ttcaacgaat gagagacaaa
     3661 ttactgggta atgatatcag cgccccttct accccagaag aatttgaaac actcacgaga
     3721 caattaagta ctatgcggtt tggaatgtta gaccccgata ctgcaacatt tcaaggagcg
     3781 acagggcttg ctttcagatt actggagcct gttgagacaa atgatggttg ggaactaaga
     3841 gcaagcaaca ttgagccgtg gaaagctgaa aagtacgaaa acggcgcagt gtacattaaa
     3901 gagaaatacg atccatatca aaagaagttt tatgaagaga tgaaggttgt cacgccgaaa
     3961 tcaatattta cttatgctcg atatactgat taccctggca cggtacaagg agcatttaaa
     4021 acgctgggta atgttcctaa tcctttaggc gttatcaatc ttacggagtt taaaaacaat
     4081 acaaaccgtt actgtgactt tgaagttgca gaggagatta gtgatgctat tgatcgtgtt
     4141 ttatctgatc agcagaacga gattgaacaa ttcaagttag cttatatgct gatcactggg
     4201 gcagatttgt ccgaggataa agccaagaag atgatggagc aactagggat catcaatctg
     4261 aaagacccta atgcgaaagc cggctacgtg acaaaggatc tacaaaagga tttcaacgag
     4321 tatcattttg atcttcttaa aaagcaattc tacacggtgt gtaaagcaat tgatttcaat
     4381 gacgaagtct ttaagtcgaa tagttctggg gaagcccgaa agtggcaaat cattagttta
     4441 gaagctaaga caaataccaa agaacagtac tttcgagaag gattgaaaga gtgtgccgag
     4501 gttatggctg cttatttgga ttttgccgag aaagttacaa ttgatccgga aaagatcatt
     4561 ttcaccttct ctcgttcgct tccaacagat ttaagctacc tagctgaggc attgcctaag
     4621 ttggcaccat acgtttctaa gaggacgatt caaagtcaga tcccgttcgt cacagatgtt
     4681 gattatgaaa acgaaatgat tgacgtcgaa agcagacaat cttacccaga cggcgagtat
     4741 ggaggtggca tcgttgagtc aaatagaaac gaagtactgg accaaacgtc gagaactgga
     4801 agatttagca agactcaaaa tggaaaatca aacgctaaag aaactaagta gtgtctttcc
     4861 tgatgcgttg aaagaaattc aagcggagtt attatcccaa gctgatttac acgatattac
     4921 ctatgctgag atgatggaat tttatagtac aagtgatcag aaaaaatatc gtgaatacgt
     4981 tgaaaagaac tacaaatcac tgaagatgta tgatgcaaaa tacaaagagt tcatcgaaga
     5041 atactttcct tctttcgact acgccaaagt gaatcggctg cttcaaatga ggtcagatat
     5101 ttttcgaatt cttgcagaga gtgcaataga tgcagatgtg aataaatatt tttcggatcg
     5161 gttagaagat atcttgcaac gttcgtatgt atcaaacgca aacgtatttg cccagatttt
     5221 gaacgtagat ttgccgaatt acttatcaaa ggaagaactt gaacgttacc tgaactatcc
     5281 ttggtcaggt aagacattct cgcgtcgttt atggggcaat atttcatctc tagaacaaaa
     5341 gctatctaac gccattacaa ctagtgtagc aagtggcgaa ggagttctcg tggcattacg
     5401 aacaatgcga gaagacccag aaatatgcga tatgttcaaa agggaggaat cgaagtacaa
     5461 caaggcgatc gaaaatcttg tgagaacaga atacgctaaa ttcgctcaag atggtattga
     5521 gaaatcttat attgaaactg gcgtagacga atacaatgtc ttaactgcaa aagatgaaaa
     5581 ggtatgttca atatgcggtg gcaaagcaaa gaacaaccca tacaagttga gtgaagcgat
     5641 cattggtgag aaccgtgcgc cttttcatgg ccgatgcaga tgtacagatg ttcctaatat
     5701 gccgaaatta ggaaaagata ttgacgaaga gtatgagcgt ttatttggcg atttattaga
     5761 tgagtttgca catgattctt ttggaattaa tttgaaacgg aggaagtaga atgaaagatt
     5821 tttttgaagc agtactaaca attaatgtaa atgctgatat tgcagaagcc tacaaaacag
     5881 ctattgagtc tgagaaccat cctaatggct tgagagacca ttggaatggc aattatgcct
     5941 acgtggttat tggcgatcaa actgttaatt atcaagataa tactccagtt gataagaata
     6001 ccgttaattt aacgattcaa ttattatctc attcattacc aaatttaaaa gaaacagttg
     6061 attggtatga aaatatggga tgtattgttg ttagaactga ctacaaagaa ggaaagtcta
     6121 gtaattaggc ttttttattt tgtccgaaat gacgttaaac tagcgcaata ctgggcttgg
     6181 ttgaatggtg gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa aacgaatcgt
     6241 ggggcgaaag gagaatgatt atgaaacaca aatcattaat gccaatgaat ttgcaatact
     6301 ttgccgaagg tgacgatcaa aagttttctt ttgatgactt caaatctttt gtggaatcca
     6361 atgaagaagc tcaaaaattt attcagtcac aatctcaaag tgttgccgac aaacaattgg
     6421 aagcttggaa acaaaataac ttagataaaa ttaaacagga taccatcaag gaatatgagg
     6481 aatctaagaa aaacaagtca cctgagcaaa ttcaactgga aaaactacaa gctgaatttg
     6541 aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc atttgttgca gaacaaattg
     6601 ctggattaga actagatgga gagttaaaag agtctatttc tcaatttatg ctaaataatc
     6661 ttgttagttc ggatacagat ttcactaaga atgctgttga aggttttaca agtgttttga
     6721 atgcaattaa tgagaaacat gcagatgcat taaaagaact acaaatgaag tctgcatttg
     6781 gtggaactca acaatcgaat aaccaggttc agcagaacaa tgaaacatta acaaatccag
     6841 aagaacaatt aggacaaatt cttcaacaat ttaactagga gagtgaaaaa ttatgaaaaa
     6901 aacatcttta aataatttag agtatttgga tatttcacca gcgattaatg ctatgcaagt
     6961 accaaataca ccttttttaa gctatttact tggtgctgga aaaacagagc cagcaaactc
     7021 gacagaaatt aaatggcgtg aatatgatat caacaacgat gattcttctg aaaaacttga
     7081 gggcggagaa tatccagatg ctgaatcagg tcgaacttgg tttaacaact atactgaaat
     7141 ttttagaaaa tcaacctctg tatctggtac attagatgct attaatgtga atggagtcgg
     7201 aaatgaatta actaatcaag tagcactacg tggtatggaa atgaaaattg atttgaaccg
     7261 aaaattgatt actggtgtaa aagctgatga aaatggttct aaaggtcgtc gaatgaatgg
     7321 gattttgaac ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg gtgcagtaac
     7381 aagaaaagat atcgatactt tatttaaatt gatgtatgaa aaaggttata tgggagaaaa
     7441 actatgcttg atttctccag atatgcagga gttaatgact gatgagttag atggaaaatc
     7501 aacaaaaatt gttcagttcg gggaaagagt aacttttgga ttgcaaattg gaaatatcgt
     7561 gtctaattac ggtacaggta ttgctctact agaaccatca ttgccaaaag gaacaattgc
     7621 cgcaatcgat actaattatg tgaaactacg tccattacgt gaatggagag cagaagaact
     7681 tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt gaatactctc ttgaatacaa
     7741 tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa taaaagggag ttagtactcc
     7801 cttttttgat aggaggaatt acagtggtaa aaaaagatga aactaaaaaa gatgaagtcg
     7861 tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct aaaactcgta
     7921 gatttatcac agcagattca aataacgaat ttatcatttc tatagatgat aaagaagcaa
     7981 ttgcaatttt ggaagatgca attgatgtta atgaaattta ggaagtgatc tgatggatga
     8041 atcgctaaaa acggaaatca ttgagtctac aaaagaagat tttccagatt tgagtgaaga
     8101 acgcataact aatttattag aaataatttt gctagaaatt gaatcataca acacttgtaa
     8161 aaatgatatt tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt ataaaataat
     8221 aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa cgattagtta
     8281 tgcttcaaca acaaatgatg ttagtgagtt acttctaggc tatggtgatt tgatacgaag
     8341 agttattggt tgtggaggat tggagttttt ttaatgaatg aagcagatat tttagaaact
     8401 acttatgaag atagttgtat tattgaaaga ctgacggaca ttgaagattc taatacaaat
     8461 attactattc aagattacaa aaaagtatat gataatccta tttcttgcgc tctttcacaa
     8521 ggtcaaatcg atggactagc agtcatagaa gatggagaga tggtaaatgt ttcaactgac
     8581 acatataaat tatttgttca tcctaagatt aaactaaaaa aaggagatcg aataacaata
     8641 actcaaaagg ccagtggctt aattttttct ctatttgcta ctaagccttt ttactatcct
     8701 agtcattgtg aagtgaattt gataggaagt gagaaaaatg ggtaatctca aatatgaatc
     8761 aaatgcagaa aagatcattg agaattttaa aaatatgact gtaattgctc aaaaagaagg
     8821 aatatccttc gttaacgatt caatgaataa agtcgttagt ttagctaaac cattaactcc
     8881 tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa aactatcaag
     8941 cggtcgaatt gttggagcag ttataaacaa tgaacattat tttaaatatg tagaagaagg
     9001 tcataggact aaaaatggtg gctttgtcaa agggaaattc atgttaactc gtgcaacgaa
     9061 tcttgcaaat atgtcttata ttcctcgaag atttaaacaa atggcaataa aaatcgttaa
     9121 gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa ttaaaagaaa
     9181 tctatcctga tgggacaata tatcttgatt cagttatgca gtcaactaaa gatttttatt
     9241 tcgttttatc agtaatggaa tctggaactg aaaatgtagg aattgatatt caaaatgttt
     9301 ctttcttaat tgatattgca ttgattgata ataaacctaa tagaaattta attaatgaat
     9361 tagtctcacg ttgtgggact ttttttaata cgattacaat cgatgagcaa atattatttc
     9421 caaaagccta tttacctgat gaagcagatg gtgttcaaca tattcgtttt acattagaat
     9481 ttccacaata tattgaatgg agtgaaagat aaatgggaga aaaaagaagt aaaaccggaa
     9541 ttatttctgt cgaaaaacct acttggtttc ctttggaaga tgagacaggg aatttcccgg
     9601 tatatggtac tgcgttgcca atgggaacag cagtaagtat caaaccaaca gctaattatg
     9661 aaacaacaca agattatggg gattcagtgg ttcaagatca atttacagct tttggtggtg
     9721 cagaagttga actggaagca aatggatata ctcataaagt tttatctaca attactggag
     9781 ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa gatggtgcgt
     9841 ttgcatatag acgtaaaaaa tcgaatggga aataccgtta tacggtcttt tataaaggac
     9901 agtttgctct agattcagat gaaacgtcaa cgattgaagg aagtaaagta agttttactc
     9961 atccaacttg gaagggttca tttgtagatg taccaggact tggatatatg tattcagttg
    10021 atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca aaagtagcaa
    10081 ttccaattga agaagcagaa ctttcaggag gtacagaata atgtcaaaat atcaaacaac
    10141 gattaaatta acgaaaaaag atgaagaagg taaatatgag caagtacaat ttaaatctgc
    10201 tgaattttta ccaggaacag ttgtagaaga tgctgcagga gttatggaag aaatgcaaac
    10261 agcgactgat aaacaatcag ttaaaaaagc tttaagtcgt gcttattcat ttattgcaga
    10321 tactttattt gagggacaat ttactggtga agattattgc aaaggaattg atgctcgtga
    10381 gattgcctca ttgacaggaa aattattgaa gtctgttact gcaggttttg atgaaactta
    10441 tacagagacg aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa gtattcgatt
    10501 acttaccgag aattagatat aaagacgcaa ttactcgaag caggttggac gttaccagaa
    10561 attgaacgta ctgacttgga tgagttaatg cgtctttttg cttttagaga tgcggtaaaa
    10621 gagcatgaag atgttgagta ctatgataat ttcactcaat tttaggaggt gatactttga
    10681 ataacgatga cctaattctg aagatgatac tagatgaatc tggttttact gccggtatga
    10741 ataatgctgt caaaaagttg aactcttttg atgaaacaat cgaaagaaaa agtagaaata
    10801 gtggcagttc attaggtagt atttggaaaa tatttgctgg aagttttcta gctagcggag
    10861 taactagaat cgtaggagct ggctttgatc taatcaaggg ttccataagt ggagcggttg
    10921 atcgagtaga tacgatgaat aacgctctac gaaatttcca aaatatggga tttagtaatt
    10981 cagaaattat gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt caaggacttc
    11041 ctactgcttt gaatgatgcg ataagtcatg ttcaacttct tgcttcttct acaggtgatt
    11101 taactcgttc aactcaaata tttaaagctt taaatgatgg aattcttggt ttcggtggtt
    11161 caactgatca agttaatgaa gctgttattc aattatctca aagtttctca aacggaaaag
    11221 tagacgcaca aacttggaac tcaatgatca acgctcaact tggacctact ctttctgcta
    11281 ttgctaaaaa gatgggaatt acaatgggag atctgaaaga aggtttatct caaggtaaga
    11341 tttctgttga agaattccaa aatcaattaa tagaaatgga taccaaaggt ggcggaggac
    11401 ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg aattaaaacc tctatacaaa
    11461 atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat agaaggatta aataaagcat
    11521 tagtagattc agatttaggt ggatttaagg gaattattga taaagtgggc agttcaatgg
    11581 aatcgttctt aaaagtaatt gctgcaaata ttcctacagc agtatcattt ttaagtaatt
    11641 tatttaacga aattcaaaaa tttggttctg ctttgaaatt catgatgccc tttcttgttc
    11701 ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa aggtatacca gcaataataa
    11761 aaagcttcaa taattttaag aatgccataa ttggcgttgg aaattcatta aagattatgg
    11821 gggcaatagc tgctgctaat tcatttgttt taattattgg tgcagtcgtt ggtgcaatag
    11881 ctgttttcgg ttactttatg gcaaccaatg aagagtttag aaacaaggtt atatctattt
    11941 ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa tataaaggac tggggaattg
    12001 atacttggaa ttctgctaaa gaaatggcat caaatgctgt tgagagtgtc aaggatgctt
    12061 ggtctggaat aaaggaatgg ttttcaaata cttggcaagg cattaaaaat ggagcaacag
    12121 gtttatttga taaaacagta gaaacatcta tgaatgcggt tgatagtgta aaaaacgcat
    12181 ggtcaaatac aaagcaatgg ttttctgata tttggcagag cataaaagat tcagcaactg
    12241 aaaaatggaa tgaaattaaa ggttctatca tggaagttgc tggtccattg attacaggtt
    12301 taaagaacgc attcttgcat gtcacttttt atttagagac cttatggaat aatctagttg
    12361 agattggtaa aaatgttttt gagattttaa aaaatgttat acttacgcca gttttgttta
    12421 ttacctccat gatttctggt ggatgggaag aaacaaaaaa caacatgatt ggtgtatgga
    12481 ataatattaa agaaagtgct ctaaatattt gggagtctat aaaaaatatt tttgttagtt
    12541 acattacaaa tatttatttt gctgcgctta atatttggac agggttcaaa cttactttga
    12601 taaacatttg gaatgaagta gtaagtcaag ctaaatcaat ttggattaat gtaaaatact
    12661 tttttattaa tctttggatt gacattaagt attttgctat tcaaaaatgg attgaattaa
    12721 aattcgggat tattcaaact tggattgatt taaaatataa tgctattact acttggaaca
    12781 atattaaaca gttcttcaaa gatacttggc agaacattaa agatatagca tacaatacat
    12841 ggatttctat aaagaattcc atgatcaata cttggaataa catcaaggaa tctttctgga
    12901 atattgttac tggaattgtt aactccgctg aaaatgcatg gactaatcta aaaaatggtg
    12961 tttcaaaagc aattaatcgg gtgaaagaaa tctttgattc attaagggaa atcaatttat
    13021 tcgaaatcgg taagaatatc attgatggac ttatcaatgg tgtagtagaa aaatggaatg
    13081 cattgaaaaa gactattaaa ggaatagctg gaagtatcaa agattctatt aagggtgcat
    13141 taggtattca ttctccatct agatggatga gggatatggt aggtaaaaat attgttcaag
    13201 gtattatcgt tggtattgat aaagaaaaaa gtaaattgga tcagacaatg actgatttag
    13261 tgaaaactcc gtcggttcaa ccagttatta cagggtctaa tagtcaacca gtggtacaag
    13321 ctaaacaaaa tacatcttca aatgcagtga atgaaattca cttgcattta aatgtttatg
    13381 gagatctacc tgattcgatg attaaacaaa tcgccaagaa gatgaaaaca gaattgacga
    13441 gacaaatgaa acgagatgct gatgcagtag gagggacatt atatgcaact taaaagagga
    13501 caatttttta tcaatcaaca ttattcttct gagtttaacg tatatattca aaataggcct
    13561 gcctctgttt cagctagtcg tgtaattgaa ttgagagaac gtgaaggcaa tgactcaatt
    13621 attatcgata aagcctatta caaaaacgtt actagaaaaa ttgaatgtta ttacaaagca
    13681 ccatcgattg atttagtgca ggaatgggaa gatcgaatta ctgaatggtt agatatgaac
    13741 tcttacagtg attttattct ttattacgat caacaatata tctatcaagc tatagtaacc
    13801 gaagcgccag aatttaaagg aacaagaaaa acagggaaca tagttccatt tgaatttaca
    13861 gttagtcttc gaccattcaa agaaaattat agtggtcgtt ttgctattca acagataaaa
    13921 gctttcgagt tatttaatcc agagaagtat gcttcaaaac cgcttattaa attgagtggt
    13981 tctggagatg cttcttttta tattaataat gataaatatg atttgaaact attagataga
    14041 gaattatata tagattctaa actagaagag tcttatcgaa agctagatga caatttagaa
    14101 catcaagatc atgtcacttt atttttagat tttccatttc tataccctgg aaaaaatgaa
    14161 atcaaatgga cgaataacat acattcgttt gagataatac caaggtggtg gagaaaagta
    14221 tgataccaag aatatatagt cctaccgaga cagatttttc tacgaatggt ttagggattt
    14281 tgaaggacac cacaaaatgt gagatatatg aagtagcaaa tggaaaatat gaattagagt
    14341 tggagtatcc attaggtact cgatttgatg aatattttga aaatgactat caaataaaag
    14401 caaagtcaaa tgatcaagaa gagtatcata tcttttttat tgatgataaa gatattgata
    14461 cctttttaga taccgtaact atttatgccc agagtcgtac aaatcggctt ggaagacgag
    14521 cggtcacttt tgctggagtc gactctaaaa ctggtcgaga agcaatggca attattgaaa
    14581 acaatatgga taaaaaatca gatatacgct tatattctga tattacaact gtatcaagta
    14641 caacttttga agcaagaaat gttttaaatt gtattgctgg tgaacaagga tctttacttc
    14701 agtattgggg cggagaaatt aaacgtgagc cttttaagct atctttatta aagcgaagag
    14761 gtcgtgataa tattggcaca atccgatatg gaaaagattt atctgggtta aaagttaagt
    14821 tagattggac aggcgtgaaa acaagaatta ttccgtatgc tgatcctcaa agtgatgcag
    14881 gtacaactag ccgaatttat ggctcgccag tagatagcga atatattaat aattatcctg
    14941 atgtatatac agaacacgtt caattcacag aagaacaggg agttaaagac gttaatagct
    15001 taaataaaat agctaagaat tatttcaaaa caattaatcc aggctgtgat aaaccgaaga
    15061 tttctattac cgttgagttc gataaattaa cggacaccga agaaggaaaa gaattcgcta
    15121 agataagaaa ttatgggtta tttgatacgt ttaaaatata tcatcgaaaa tataaactat
    15181 attttgaatc gaaagttagc ggtgttcaat atgattcatt atctgaaaag gttttgaaat
    15241 tggaagctgg ggatgctcaa gttgcttttt atcaacaaca agctgttact attcaagata
    15301 aattaaaaga ttatgctacg aataattata tgagtagttt taatgattat gtgtcctcta
    15361 tgattgcagg tcaaggaaat gcaggcggtt atgtagtttt atggccaaaa gaaaaacctt
    15421 caaatatttt tataatggat agccctgatt taaacaaggc aaaagaagta ttaagaatga
    15481 acaaaaatgg aattgccttt tctaaaaatg gttggaatgg tccttttaat tccgcttgga
    15541 cattggatag tatattcaat gctaatttta ttcaaacagg attaataaag gcagatatct
    15601 ttcaaaactc ttttaataaa actggagatg tattgaaatt agttaatgga ctacttcaaa
    15661 tttggaataa caaaaagaaa ataatggagt taaccaaaaa aggaatggag ttttggaatt
    15721 ctaatagttc aattggaacg attggaacaa ctgattctgc tggtaatcct tttcctggag
    15781 cttctactcc cacacctatt gaagataatt ctttagttat tcgtacaaat ggagacggca
    15841 aatatatttt gatttctcct aaagttggta aaggattagt tttattagga aatggtaaag
    15901 caatttattt tggagactta gatgtacaag gcaaactcac agttaacgga aaagaaatca
    15961 cagggaacaa cagtggagga agtgatcctg gaactatccc tcctcaattg acgacggaag
    16021 ctgaaaaaag agcatggaaa atttggacaa tgctgaaagc tcgtggctat tctgaatatg
    16081 cagctgcagg tattctagga aatattcagg gggaggttgg agcaagtatg aaccctgata
    16141 cagaacaact tggtggtcca gcttatggga ttgttcaatg ggatggttct gcttatccgt
    16201 tagtcggatc accaacttgg aacggacgag agtatgttca gcgtttgatg aacaccgcag
    16261 ggattcaaga agattatcga agtattgaag cccaagtaaa attattagat tggtgtatgt
    16321 tcaacggtca atggctcgga aaagtaaatc caaccacagt atcaggattt aaatcgatca
    16381 atgatgccaa aagtgcagcg tatgcttttg aaatgaactt cgaacgccca gcttctgcac
    16441 atccagaacg ccaaaattat gcccaatctt ggtataacaa attacatgga ttaactagtc
    16501 cagaacctgg agggaatttc atttgtccaa ttcaaaaacc agtgacagtt acttcagaat
    16561 gtggatggag aactagtcca ataaatggcg gccaagaatt tcataatgga attgatcttg
    16621 taaatggaaa tcctaataca cctgtttttg cagcattaga tggggaagtt gttcaggctg
    16681 gtgctaatta ttatgactgg tatggtaatt acgtggttat taaacataat aatgggaagt
    16741 ggacaggtta tgctcatttg tctcgtattg atgtttctgt tggacaaaaa gtccagaaag
    16801 gtgctcaaat aggcttgatg ggaacaactg gtccatctac aggagaacat ctacattttc
    16861 aaattatgaa aaattattgg ccacagccag ttgttgattt tgagaatcca agaaattata
    16921 tccaatttta aggtggtgat tctatgagta aatggaatgt cgttttaagt acaacagaac
    16981 catataatta tgtggggatg attcaagttc gacaaggcaa taagaataca gaggttatgg
    17041 aagcgactat agttgaaaat ggtcttccct acgatttatc agaatgtaag gtatattttg
    17101 aatcagttgt aggtgggaaa tatccagtcc aattagaaac aaaaattgtg gatgctaaaa
    17161 aagggaaaat taa
//
LOCUS       DAFEZB010000057        17107 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFEZB010000057
VERSION     DAFEZB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.057"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            7..1398
                     /locus_tag="LP859_002026"
     CDS             7..1398
                     /locus_tag="LP859_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL3029958.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="LP859_002027"
     CDS             1414..2454
                     /locus_tag="LP859_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3029959.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="LP859_002028"
     CDS             2473..3558
                     /locus_tag="LP859_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL3029960.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="LP859_002029"
     CDS             3591..4553
                     /locus_tag="LP859_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL3029961.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="LP859_002030"
     CDS             4546..5979
                     /locus_tag="LP859_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL3029962.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="LP859_002031"
     CDS             5976..7046
                     /locus_tag="LP859_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3029963.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="LP859_002032"
     CDS             7033..8454
                     /locus_tag="LP859_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL3029964.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFITIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="LP859_002033"
     CDS             8467..9474
                     /locus_tag="LP859_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3029965.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="LP859_002034"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="LP859_002034"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBL3029966.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="LP859_002035"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="LP859_002035"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL3029967.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="LP859_002036"
     CDS             11608..12285
                     /locus_tag="LP859_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL3029968.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="LP859_002037"
     CDS             12275..13417
                     /locus_tag="LP859_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL3029969.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="LP859_002038"
     CDS             13430..14407
                     /locus_tag="LP859_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3029970.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="LP859_002039"
     CDS             14407..15315
                     /locus_tag="LP859_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBL3029971.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="LP859_002040"
     CDS             15316..16068
                     /locus_tag="LP859_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL3029972.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="LP859_002041"
     CDS             16073..17020
                     /locus_tag="LP859_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL3029973.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6165 a         2190 c         3089 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattacgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DAFEZB010000058        17005 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFEZB010000058
VERSION     DAFEZB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.058"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..124)
                     /locus_tag="LP859_002042"
     CDS             complement(<1..124)
                     /locus_tag="LP859_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL3029974.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="LP859_002043"
     CDS             complement(228..539)
                     /locus_tag="LP859_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL3029975.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="LP859_002044"
     CDS             complement(541..738)
                     /locus_tag="LP859_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL3029976.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="LP859_002045"
     CDS             complement(1132..2859)
                     /locus_tag="LP859_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL3029977.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="LP859_002046"
     CDS             complement(2852..4045)
                     /locus_tag="LP859_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3029978.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="LP859_002047"
     CDS             complement(4232..4876)
                     /locus_tag="LP859_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3029979.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="LP859_002048"
     CDS             complement(4970..5104)
                     /locus_tag="LP859_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL3029980.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LP859_002049"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LP859_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL3029981.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="LP859_002050"
     CDS             complement(7235..7708)
                     /locus_tag="LP859_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL3029982.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="LP859_002051"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="LP859_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL3029983.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="LP859_002052"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="LP859_002052"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL3029984.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNSLYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="LP859_002053"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="LP859_002053"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL3029985.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="LP859_002054"
     CDS             complement(11755..12894)
                     /locus_tag="LP859_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3029986.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="LP859_002055"
     CDS             complement(13203..13916)
                     /locus_tag="LP859_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL3029987.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LP859_002056"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LP859_002056"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL3029988.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="LP859_002057"
     CDS             15105..16637
                     /locus_tag="LP859_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3029989.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="LP859_002058"
     CDS             complement(16828..>17005)
                     /locus_tag="LP859_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3029990.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2908 g         5324 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca attcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DAFEZB010000059        16931 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFEZB010000059
VERSION     DAFEZB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.059"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..439
                     /locus_tag="LP859_002059"
     CDS             <1..439
                     /locus_tag="LP859_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3029991.1"
                     /translation="SGATLGLIIFIAFLAKSDQLKVLGKASLVPGLFNINEPIIFGMP
                     IVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSPVGIGAFVGSGGDWKA
                     AVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK"
     gene            complement(523..2028)
                     /locus_tag="LP859_002060"
     CDS             complement(523..2028)
                     /locus_tag="LP859_002060"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL3029992.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(2120..2710)
                     /locus_tag="LP859_002061"
     CDS             complement(2120..2710)
                     /locus_tag="LP859_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3029993.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2889..4535
                     /locus_tag="LP859_002062"
     CDS             2889..4535
                     /locus_tag="LP859_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3029994.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4584..5303
                     /locus_tag="LP859_002063"
     CDS             4584..5303
                     /locus_tag="LP859_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL3029995.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5782..6729
                     /locus_tag="LP859_002064"
     CDS             5782..6729
                     /locus_tag="LP859_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3029996.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6741..7661
                     /locus_tag="LP859_002065"
     CDS             6741..7661
                     /locus_tag="LP859_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3029997.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7702..9159
                     /locus_tag="LP859_002066"
     CDS             7702..9159
                     /locus_tag="LP859_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3029998.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9504..10118
                     /locus_tag="LP859_002067"
     CDS             9504..10118
                     /locus_tag="LP859_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL3029999.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            10111..11835
                     /locus_tag="LP859_002068"
     CDS             10111..11835
                     /locus_tag="LP859_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3030000.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11832..13283
                     /locus_tag="LP859_002069"
     CDS             11832..13283
                     /locus_tag="LP859_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3030001.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13756..15909)
                     /locus_tag="LP859_002070"
     CDS             complement(13756..15909)
                     /locus_tag="LP859_002070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL3030002.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15911..>16931)
                     /locus_tag="LP859_002071"
     CDS             complement(15911..>16931)
                     /locus_tag="LP859_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3030003.1"
                     /translation="FNDLQTAILTGELPVDSQLPTEKELSRTYKVSRITSKRALTELE
                     QLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFSKGLNPVMQD
                     KQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKEFPVVILDKK
                     IYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRYLGYLEALDK
                     AGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQHGYHIPEDFS
                     IIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSKFPVSFIKRQ
                     STKEK"
BASE COUNT         5379 a         3023 c         3381 g         5148 t
ORIGIN      
        1 atcaggtgcg actttaggat tgatcatctt tattgcattc ctcgcgaaat ctgatcagct
       61 gaaagtgtta ggaaaagcct cacttgtacc aggattattc aatatcaatg aaccaatcat
      121 tttcgggatg ccaatcgtct acaatcccta tttagcggtt ccgtttttct tagctccgat
      181 ggcttctgct acactcgctt attttgccat tcggctggaa atcgtcaaac cgatgcttgc
      241 acagatgccg tggccttcac cagtcgggat cggagcattt gttggtagtg gtggtgattg
      301 gaaagctgct gtgttagcag tcatttgtgc aatcatcgca ttcattatct ggttcccatt
      361 tatcaaattc tatgataata aattattaaa tgaagaaaga gaaaaggcag cagaattagc
      421 tagtgaaaag ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa tcacagtccc
      481 tttttttctt agaaatggga aaccactagt atataaatac cattattctt caatcaaccg
      541 cttcacgatt gcataatccc cttcataagg ttcatctaca ccattgattt tttgataggg
      601 atccacgcct tttcccgcaa taacgactga atcttgcgga gcgctttctg ctaaagcgcg
      661 tcgaatcgct tcaggacgat ccatttcata atgaacgatc aaatctggat tagtaattgc
      721 ttcattgatt tcttccgcga tttttttcgg atcttcaaat gctgggtcat ctgccgtcaa
      781 aaaggcgatg tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac gggagattgc
      841 tttgtttcct gggctgccta atacaacaat tacacggcca tctggatgtt catttttggc
      901 aaatttcagc aatgttttca agcttagata attatgtgca tagtccacat agacatgcgc
      961 tccattggat tggatcaatt gatccattcg gcctggtaca cgagcttctt ttaatccgtc
     1021 ttgtgcatct tgactggagg cacctgttag tgctgcggca atgatcgcac tggctgcatt
     1081 ttcttggttg aatcctccag ccagcaaaat atcataagca gtatcgcaga tagctaagcg
     1141 atcttcatga gaagataaag taaaaccgtg agtaccttta tctgaacgga tcacttgata
     1201 attgctttct tccgaacgac cataagtgat cgttggaatc tggaatagat cggctgtttc
     1261 cctaagtaaa tcaaaatagt cactttcatg atttaaaaca actgtttttg aattcaagat
     1321 caattgtcgc ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg gactgatatg
     1381 gtcaggcgag atattcaaga agattccgac atctaaagtc aagccgtata cgcgttgtgt
     1441 tttgtaagct tgtgaagaaa cttccatcac tagatgagtc ataccatttt caactgcttc
     1501 tgacatcata cgatataaat ctaatgactc aggcgttgtc aaatgggatt tgaaatatgt
     1561 tttgccatct aacgttgtat tcatagtaga aagcaatgct gtttttttat ttgttgtgtg
     1621 atccaagatt gcttttgtaa agtacgcagc agttgtttta ccttttgttc cagtgaatcc
     1681 gatcactttc aatttatttt gcggatagtc gtaaaaagcc atagcaagaa gcgccattgc
     1741 tttacggata tccgttacga tgattcctac tgctgaattt tcgtatggtt gttctgatac
     1801 ataatattga ataccctgtt cgattgctga atccaaatat tcttctttga aattcattcc
     1861 tttacagaaa aacaaagtag aagcatcagc ttttcttgaa tcataactta actgaagaaa
     1921 ttcagctttt tctggtacat caagatgcca gcctcttgtt gaaacaaatt cttttaatag
     1981 attttctttt aatagtaaat gacggatgtc ttctaaagag aagctcattg attgatccct
     2041 cgctttattc aatttcttgg tttattatag cacactcttt ttaatatatc gtttgtttcc
     2101 gctatgacaa aatccagctt tattcaaatt ttcttgacac aggggacaag cggatgagta
     2161 ttttcataaa caatgaactg actgtaatag ataggagaac gaatagtgtc tccaacaatc
     2221 caaaaacata ataattcaaa aagacgataa gactattggt aagtaaaagt ccttgcggca
     2281 ttggaagccg aaaaaatttg tggaaaataa tcggcggtat cgttataccg ccatttgagg
     2341 ccccgactgt gtacaatgtg taaaccccaa gactgaacaa aagacttcct gaaatcagtg
     2401 aaaagaaatg tgaggagatc atttccactt ttggaacaat ctctaaaaca acaggaaaaa
     2461 gcagacttcc cgctacagtc ttaaaaaata ctgattttcc taataggaca gcggctagaa
     2521 gcagcatcat aagattgatg ctccaaacag taactgagcg ttggatagaa aaggccgttt
     2581 caactaaatg tccgatggcg cccactccgg cggaagctat atcgtgaggg agcaagaacc
     2641 aattcaatcc aactgtgata aagaaaagac ctaaaaatat aacgggtagt ttttttattt
     2701 cttgtgtcat tgatatctcc tataaatgtt agccataaaa ggctgtatat ttctttactt
     2761 taccaaaaaa acgaaaccga attcaaaaaa acaattgttc ttataaaagt tatataaaat
     2821 cgattgccag tttctgtttt accaattaaa atgatagtaa tgttttgtag aaagttggaa
     2881 tctgaccgat ggataatcaa agaaaaccag cattagatca attaacaccg caagaaaaaa
     2941 tggcacgagg ctctgcttgg atgaccgcca gtaatatcat ttcaagattg ctaggcgctg
     3001 tctatatcat tccctggtat gcttggatgg gcgaaaatgc taaggctgcc aatggattat
     3061 tcaatatggg atacaatata tatgcattgt ttctactgat ctctacagca ggaatacctg
     3121 ctgcgattgc aaaacaaact gcacgctata attcattgaa tgaatatggc acttcacgac
     3181 gattatttat ccgtgcatta cagatgatgg gcgggttagg cctgttgttt gctttgttca
     3241 tgtacatcgc ctctccttgg ctagctcatg cttctggtgg cggagaagaa ctgattccaa
     3301 cgatgcgttc tttgagtatc gctgtattgg tttttccttg tatgagtgtg atacgagggt
     3361 atttgcaagg caatcaagaa atgatgcctt atgcgctgtc gcagattgta gaacaggtgg
     3421 cacgtatttt ttatatgcta ttggctacat tcatcatcat gaaagtcatg gatggtgact
     3481 atgtgacggc ggtcacgcaa tcaactttcg cggcatttat cggtatgcta gcaagtattc
     3541 ttgtcctttt gtattttttg aagaaacatc aagcgtatac atcggcgttt attcactaca
     3601 gcgaaaacaa aataaatatt gcaacgaagg aattgttgtt agataccgtt aaagaagcga
     3661 ttccgtttat tattgtgggt tcaggggtaa cgatcttcaa attagtcgat cagttcactt
     3721 ttatgaagat catgtctgca tccaccgagt attccaatgc acagttactg gatctttttt
     3781 cgatttttag tgccaatcca gataagttga cgatggtagt tattgcattg gcaacatcga
     3841 tcgcgtctac tgggttaccg ttgattacag aagctgttac gttgaaagat cgagtaggtc
     3901 tggcaaaatt gacaagcgga aatttacagt tgttttcatt ttttatgttt ccggcgacat
     3961 ttggtgttat gttacttgcc tatccgctaa atacattatt ttatacaccg gattctctag
     4021 gaagtaatgt actgatccaa gcaagttttg taggactctt cctaggacta tatatgctag
     4081 tttccaacat gctgcaagga atgtttgaaa acaaggcggc aattaattat ttggttattg
     4141 ggtttctagt gaaactcctt cttcaatacc cagccattcg tttatttgaa gtatacggcc
     4201 ctttattggc gacaatgatc ggatttgctg tgtcttgtac cttgatctta aagagaatcc
     4261 atgaagtagc ccgctttaaa ccaagttttg tctggcgcag gacattgctg atctttattc
     4321 ttaccttgat tatgcttttg gcttcgtgga tcacaaagat gatttttggt gcttttttaa
     4381 acgaagatag caaattccag tctctattgt tgattcttgt agtagcaggt ataggggctt
     4441 tagtgtatgg ttatttagct ttgaaaatcc gcttggcaga aagactacta ggaccaggaa
     4501 tagcgcgttt acgacataaa ttaaaaataa aataagtgaa tcagaagagc tagattgatg
     4561 ctcttttgaa atgggggaaa cgcatgcgtt tagataagtt tttagcagat atgggacttg
     4621 gcagccgcaa agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc aatggacaag
     4681 tggtgaaaag cgataaagcg caagtaaatg aattcgaaga tgcggttact tatttaggag
     4741 aaatcattac gtatcaaaaa gatttttact acattttgca taaacctgca ggagtcattt
     4801 ctgctactca ggataatcat gatcaaaccg taatcgattt attggaagac caggattatc
     4861 gggaagatct ttttccagtc ggccgattag ataaagacac agagggcctc ttgattttaa
     4921 cgaatgacgg ggtgtttgcc catcagttac tatcacctaa aaaacatgtg gagaaagaat
     4981 atctagctga ggttagcggt ataatgacag aagaagatat ccgtttattt gcagaaggac
     5041 tgattattga tggcgatgag cagacattgc cggcaaaact ccaaattgat caagtagatc
     5101 aagagacaga aacttcaatt atccgtctga ttttgcatga aggaaagttt caccaagtta
     5161 agcgaatggt caaagcagta ggaaaagaag tcttatattt aaaaagaatc cgtatgggaa
     5221 aattttggct gcctaaagat ttgaataaag ggcaatatcg acccatgacc aaagaagagc
     5281 tagaacaagt gagagaaaaa taattgaatg gatagctaaa agaataagca gagtagttgt
     5341 agcgccatct gaaaagcaga cttgtccgtc tgactttcgg atggcttttt tgtgaaattt
     5401 cggcttattt ccgtagaagc tacttctgga acaccgtttg ttcggaataa gggactgtga
     5461 caagactttt gttacagtcc ccattgttcc ttgtgactca gagttagata gtaaggacac
     5521 gatctcccta atactcaggc caaaacgctg aattcacaac ctctgataaa cggtgttcaa
     5581 tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttcccgtt
     5641 tttttctcta atactcaggc caaaacgctg atttcacaac ctctcctata ctttgtttca
     5701 ttttccctat actttgttta tttattctca ttattgaaag cgctataatt acgttgcgaa
     5761 tgaatcgaaa ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt ttttatgaat
     5821 ctatggcgtt ataaagcact tgtcctgatg gctgtaccgg gtatgctttg gatgattttt
     5881 ttcttttaca tacctgtaat ggcgaatgtc gtagcattca aaaattttca tatttcagca
     5941 gatgggttct tggctagctt gagggaaagt ccttgggtgg gttgggaaaa tttcaaattt
     6001 ttattttctt cggatcaagc atttctcatc actaaaaata caattttata taatgtagca
     6061 tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag cgagttgaga
     6121 aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg
     6181 gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag
     6241 tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg
     6301 tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt
     6361 gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc
     6421 aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta
     6481 acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct
     6541 aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc
     6601 ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc
     6661 ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca
     6721 ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt
     6781 tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta
     6841 ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc
     6901 acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc
     6961 agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta
     7021 cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc
     7081 cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca ccagggatgg
     7141 tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga
     7201 tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt
     7261 cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat
     7321 ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg
     7381 cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat gataagctag
     7441 ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt
     7501 caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa
     7561 tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc
     7621 attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc
     7681 ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca
     7741 gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa
     7801 gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat
     7861 caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc
     7921 aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa
     7981 aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct
     8041 gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca
     8101 tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc
     8161 tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt
     8221 gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa
     8281 ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat
     8341 cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc
     8401 aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa
     8461 caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat
     8521 acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca
     8581 aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct
     8641 caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta
     8701 gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc
     8761 gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt
     8821 tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca
     8881 caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa
     8941 acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat
     9001 atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat
     9061 gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa
     9121 gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg
     9181 ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt
     9241 tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct
     9301 tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta
     9361 accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct
     9421 ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa
     9481 acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga
     9541 tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca
     9601 tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg
     9661 agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag
     9721 gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac aatttatatt
     9781 tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct
     9841 tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct
     9901 ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc
     9961 taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga
    10021 tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc
    10081 gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa
    10141 ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg
    10201 actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac
    10261 atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt
    10321 aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac
    10381 ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat
    10441 gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt
    10501 gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa
    10561 ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct
    10621 atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta
    10681 gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga
    10741 acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga
    10801 caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg
    10861 gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta
    10921 ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt
    10981 atcattattt tagacaatat actgcttatc tttctttata aaacattcaa acgatattca
    11041 cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg
    11101 cggatcgaca taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa
    11161 atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa
    11221 gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta
    11281 gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat
    11341 gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa
    11401 gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta
    11461 gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc
    11521 cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg
    11581 ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga
    11641 aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg
    11701 gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc
    11761 agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca
    11821 ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga
    11881 atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag
    11941 agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg
    12001 atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc
    12061 ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta
    12121 tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa
    12181 gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa
    12241 tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag
    12301 aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa
    12361 gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc
    12421 aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg
    12481 gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg
    12541 aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac
    12601 agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa
    12661 caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattgg
    12721 aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc
    12781 cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg
    12841 atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc
    12901 aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca
    12961 gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac
    13021 tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa
    13081 aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac
    13141 aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca
    13201 acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc
    13261 gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac ttataaaaaa
    13321 ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag ctttttcatg
    13381 acctctcgat aaacggagtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    13441 aatgtggaaa gccattttca caaaattttt cttaatactc gaaactaaac agctttttca
    13501 cgacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaatggc
    13561 aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga acgctgattt
    13621 cacaacctct caataaacgg tgttcaaaag cttgcatctg cggtaataag accaaccaaa
    13681 cgaaaaatat gaggatctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt
    13741 tttcacaacc tcttgttaat tatagtttga taaggaaaaa ggcaaatctt ttgcttgata
    13801 tttcttaggg tgagggatga tccctaattg gtaagagatc gtgccgcctt tcagcaaatc
    13861 ttgatgggta aaatataatt tcgtatattc tttgtccatc cgtttgattt tatggataaa
    13921 ttgttgctgc ggccgattag gcgttgcttc aatcgtcaat tcctttccat ttgacaaaag
    13981 caaaacagcc ttgtcaatta atggcatacc gataacatat tcaccggttc caggcgtgac
    14041 aggataaaat cccagactat tgaagatata ccaagcggcc atacttccat tgtcctcatc
    14101 tccagggtaa cccattggag aatcgtcgaa ggtctgagtc atcaactgtt taagcagtgg
    14161 ctgtgccatt tcaggttgtc ctatataact gaacaagtaa ggatagtgaa aacttggctg
    14221 gtttgaaata gctaattgtc caaattctat cgcggccatc tcactcattt cgtggatctc
    14281 aaaaccatat cctcctgggt caaacgttgg gggctgattg cacagctcaa tgagtttttc
    14341 tttatatctc tcactgccac catattttcc gatcagtcct gaaaaatcgt gataaacagc
    14401 aaagctgctt tgccaagcac ttccttctgc ataatcttgt ccccaacgat tagctgaaaa
    14461 tggttctctg aacttgccat ctttatcttt tgaacgcata aatcctgact catagtcaaa
    14521 gatattttga tagttcaatg attgctgctg atatttcttc gtcatttcct cttcttctag
    14581 taccttggca acttggctaa tacaaaaatc gctataagca tagtctaatg tgtgattgac
    14641 tgattcgtgg tagtgattag gaacatagcc atatgtcaag taatcttctg ttccttggcg
    14701 tccataattt ggatgtttgc tttgaaccgt agcacctttt tccattgccg ttagaaattc
    14761 aggcatcaag tctcgtcgga tatcttttac agcagcatct gcaatgaccg catcgatcaa
    14821 tgtcccaggc ataagtccac gttcatcagg agacagccat tttggcaaat agccactttc
    14881 tctatagctg ttcaaaaaac cttccagcat ttcttcgtat ttttctacag caatcaaaga
    14941 ataaagagga taaaccgttt tatacgtatc ccaaaaccca ttatttgtat agagaacacc
    15001 aggttttata gatcgactag tcgtatcata gtggattttt tgcccactag gatcaatttc
    15061 ataaaatgtt tgtggaaata aaaacacccg atataaatta tgataaaacg tccgcaattg
    15121 ttgttgatca tggtgtgtga gctgtatacg atcaaaatag gactgccatt gttcttgact
    15181 gtgagcaagg taagtctctt tgctttggtt ttgttctagt tccaggttat aagctgcttg
    15241 ttccgagctg ataaaagatg tcccaaatcg gatagtttgt ttttttgtct gttcaaaata
    15301 caacgggatc gagtcgctat ttttgttgga aacagctaac tcatcaattg ttaaaggata
    15361 ttcaaatctc aatataaaat aaaaagaaaa atcaggatct tctgaaccag caaaatgtgt
    15421 gatttttcct tccactgtgt aaggatctat aacaaagagc tgatggcgtc ccggaaagga
    15481 taaaagtaaa ccgctgtcat ttttgctgta atcaatagat aagattccgc catacatgct
    15541 aggtatcaat gtagaacaga tgccataacg taattgacag atcgacagat gagtcgggca
    15601 aaatatactt tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt tctcctgtag
    15661 ttttccattg atcggcgtaa acagcatatg actgaaatca cccatccaag gacttggttg
    15721 atgggtaaga cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc agctgccgtt
    15781 ttgatccgta gtttgcggtg caaaataatt catcccaaaa ggaacgccag tataaggctg
    15841 gcagttccca ttagagaaat ttggctgatt cgctgttgca tgtctagtat ctatttgacc
    15901 gatattcatt ttatttctcc tttgttgatt gtcttttgat gaatgaaact ggaaatttac
    15961 tatcggatgg tcgatctttt gtctctaacc aagtgaccaa agcatctccc gcaagcttac
    16021 ctaactgttc aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga gctgctgctt
    16081 ggatatcatc aaatccgata attgaaaaat cttcagggat atgataacca tgctgtctca
    16141 agagattcat ggtctggata gcgacaacat cgttttcaca aacaaaagca gttacttcat
    16201 acttagtgat ataagaaagc aattcatcta atgtcgcgct tttgtcatcc agtgaagtgt
    16261 ggaaagtcaa accagctttg tccaatgctt caaggtaacc aaggtaacgc tgtcgaacgg
    16321 attgtggatg agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc tgttcgatca
    16381 aataagaggc agcaagcgca ccgccttgga aattatcgga aagaacggta ggaaaaggta
    16441 actcatagat ttttttatct aatatgacga cagggaattc tttcaatgaa agctcaaaaa
    16501 gtaagtcaag atgttgttct gtatcaagag cataataaat caatccgtca aattcattga
    16561 ttagctcttt tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg acatcaatct
    16621 gtttatcttg catcacaggg ttcaatccct tgctaaaatc accaacagac aaatcagcga
    16681 catatggaag aagaaacaga atccgtcgtg tttttgtagg agaagcaaga atctgttccg
    16741 gagaagcgga tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt cctaattgct
    16801 ctaattcagt caatgcacgt ttggaagtga ttctgcttac tttataggta cgtgaaagtt
    16861 ctttttcagt tggtagttgg ctgtctacag gtaattcacc agttaaaata gccgtttgca
    16921 aatcattaaa t
//
LOCUS       DAFEZB010000060        16823 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFEZB010000060
VERSION     DAFEZB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.060"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(39..405)
                     /gene="ssrA"
                     /locus_tag="LP859_002072"
     tmRNA           complement(39..405)
                     /gene="ssrA"
                     /locus_tag="LP859_002072"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(576..1349)
                     /locus_tag="LP859_002073"
     CDS             complement(576..1349)
                     /locus_tag="LP859_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3030004.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1493..1843
                     /locus_tag="LP859_002074"
     CDS             1493..1843
                     /locus_tag="LP859_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL3030005.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1824..2540
                     /locus_tag="LP859_002075"
     CDS             1824..2540
                     /locus_tag="LP859_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3030006.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2540..3988
                     /locus_tag="LP859_002076"
     CDS             2540..3988
                     /locus_tag="LP859_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3030007.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4058..4567)
                     /locus_tag="LP859_002077"
     CDS             complement(4058..4567)
                     /locus_tag="LP859_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030008.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4557..5282)
                     /locus_tag="LP859_002078"
     CDS             complement(4557..5282)
                     /locus_tag="LP859_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030009.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5540..6880)
                     /locus_tag="LP859_002079"
     CDS             complement(5540..6880)
                     /locus_tag="LP859_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030010.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(7047..9167)
                     /locus_tag="LP859_002080"
     CDS             complement(7047..9167)
                     /locus_tag="LP859_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL3030011.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9397..10575
                     /locus_tag="LP859_002081"
     CDS             9397..10575
                     /locus_tag="LP859_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL3030012.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10591..11349
                     /gene="aroD"
                     /locus_tag="LP859_002082"
     CDS             10591..11349
                     /gene="aroD"
                     /locus_tag="LP859_002082"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL3030013.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11372..11857
                     /gene="ybaK"
                     /locus_tag="LP859_002083"
     CDS             11372..11857
                     /gene="ybaK"
                     /locus_tag="LP859_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL3030014.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11928..13181)
                     /locus_tag="LP859_002084"
     CDS             complement(11928..13181)
                     /locus_tag="LP859_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3030015.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13298..14647
                     /gene="gdhA"
                     /locus_tag="LP859_002085"
     CDS             13298..14647
                     /gene="gdhA"
                     /locus_tag="LP859_002085"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL3030016.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14759..16105
                     /locus_tag="LP859_002086"
     CDS             14759..16105
                     /locus_tag="LP859_002086"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL3030017.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16413..16799)
                     /locus_tag="LP859_002087"
     CDS             complement(16413..16799)
                     /locus_tag="LP859_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3030018.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5407 a         3035 c         3443 g         4938 t
ORIGIN      
        1 aaggatttaa gcatctagcg aaatctgctg aaatctcaca atggagacgg cgggagtcga
       61 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      121 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      181 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      241 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
      301 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
      361 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
      421 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
      481 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
      541 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
      601 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
      661 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
      721 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
      781 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
      841 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
      901 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
      961 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1021 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1081 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1141 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1201 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1261 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     1321 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     1381 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     1441 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     1501 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     1561 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     1621 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     1681 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     1741 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     1801 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     1861 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     1921 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     1981 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2041 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2101 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2161 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagtagttt
     2221 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     2281 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     2341 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     2401 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     2461 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     2521 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tctgcggaat
     2581 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     2641 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     2701 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     2761 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     2821 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     2881 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     2941 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3001 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3061 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3121 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3181 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3241 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     3301 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     3361 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     3421 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     3481 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     3541 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     3601 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     3661 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     3721 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     3781 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     3841 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     3901 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     3961 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4021 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4081 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4141 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4201 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4261 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4321 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4381 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4441 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     4501 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     4561 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     4621 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     4681 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     4741 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     4801 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     4861 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     4921 aaaaggaata atttggtttt agaaagacca gtcaggtgcc aattgatcaa ttcctgcttg
     4981 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5041 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5101 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5161 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5221 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     5281 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     5341 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     5401 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     5461 gcagcgacaa taattttggg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     5521 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     5581 tagtcagttg ggtagtgttc ttagttttct tgaaatattt tcctcgatat aaaaagattc
     5641 gttctcggta gttttgaaag tttctaaagc cataggcaat tcgttttagt actttgatat
     5701 ggttgttcaa acattctata gggccgttgg aatagggaac tcgcaacgct cgtttgattt
     5761 ctgttcgata tttcttgaag gtcgtgaaca ctggttgata gtgctctggc aactccttgt
     5821 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata gcggaaagaa
     5881 aaacctggta gacttcgtag cctcgcttta aggagtcatc ataatataat agccgatcga
     5941 ctatctccgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgaccat
     6001 tcaattccca cgcattcttt tgaagtagtc tccagtattt ctttaagtgc ttcgaccgac
     6061 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaag gcccgaccga
     6121 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     6181 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgtacttgt tcacggactt
     6241 ttctagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     6301 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacgtttt
     6361 tcaccgattt gaattcatcg aaacacacca cttctggtaa cggctggttc aaagaaacgg
     6421 ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac
     6481 gtgcaatcaa agacatagaa ataggctcac ttaatagctc aagaatggct tgtttgactc
     6541 tgcgggcaat ggagcagcga cgatcgctaa ctgaatcttc tgctaaaaaa gtctttccac
     6601 agtgtttaca tttgaagcgc tgtttgttaa tccgcacaat ggtcttgtag ttactgacat
     6661 cattcaataa gatggatact tttttccagc cccatttaat gatcgtttgc ttattttgat
     6721 agtggcaatg gaagcattcc tctggttgat aggtcaatgt gccatcaaaa acaaaacatg
     6781 tttcgtcatt aatgcgctct ttgcgaaagg catcttcatg aaaggtaaga tttaggtcta
     6841 gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat
     6901 ggttttcgtc gactttattc taaaggattt caaggtgtct gtttgcgttt attagaaaaa
     6961 agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat cctaattttg
     7021 tcgctgctcc ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt
     7081 tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag
     7141 acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc
     7201 ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga
     7261 agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt
     7321 gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta
     7381 ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa
     7441 gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt
     7501 gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg
     7561 tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt
     7621 tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt
     7681 ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca
     7741 tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg
     7801 gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct
     7861 tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa
     7921 aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc
     7981 aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa
     8041 tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg
     8101 aataccgcac taatgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta
     8161 ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc
     8221 atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa
     8281 ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga
     8341 ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata
     8401 taaatcacat tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt
     8461 tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg
     8521 tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg
     8581 accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata
     8641 aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct
     8701 cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata
     8761 aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga
     8821 ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct
     8881 cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc
     8941 gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta
     9001 gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg
     9061 aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa
     9121 aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt
     9181 tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca
     9241 tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat
     9301 taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt
     9361 tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga
     9421 gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc
     9481 caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa
     9541 ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca
     9601 tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt
     9661 ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc
     9721 ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta
     9781 tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga
     9841 ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga
     9901 aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta
     9961 aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca
    10021 gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct
    10081 gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt
    10141 ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc
    10201 attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac
    10261 atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca
    10321 aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt
    10381 attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag
    10441 gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat
    10501 ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt
    10561 aaaaataacc attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga
    10621 agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt
    10681 gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat
    10741 cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt
    10801 gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata
    10861 tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg
    10921 gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag
    10981 tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct
    11041 gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca
    11101 gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat
    11161 gcttttttct ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt
    11221 ctggttggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact
    11281 tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa
    11341 ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa
    11401 tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga
    11461 cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat
    11521 atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg
    11581 agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat
    11641 gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat
    11701 aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat
    11761 cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc
    11821 attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag
    11881 gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc
    11941 ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca
    12001 gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc
    12061 tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc
    12121 tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct
    12181 tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga
    12241 aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg
    12301 gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa
    12361 caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt
    12421 gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta
    12481 tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa
    12541 agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc
    12601 gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta
    12661 gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac
    12721 tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact
    12781 cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata
    12841 atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt
    12901 acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta
    12961 tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc
    13021 gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt
    13081 tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt
    13141 gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag
    13201 tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag
    13261 ctaaaatgag ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt
    13321 tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc
    13381 aatcgatgaa ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga
    13441 aaagaatatc ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg
    13501 gcaagatgat gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc
    13561 tatcggccct tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa
    13621 atttttggct tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg
    13681 aaaaggcgga agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg
    13741 tcaaagtttc atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg
    13801 agatatcggt gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa
    13861 aaaatatgat actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg
    13921 tacggaagct actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa
    13981 agattcattt aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc
    14041 aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta
    14101 catctatgat ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag
    14161 acaacgtttg acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc
    14221 tgtttggaca ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat
    14281 agacgaaagc ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc
    14341 taatatgcca tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg
    14401 tccaggaaaa gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa
    14461 cgtccagcga ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga
    14521 aacgatttac catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct
    14581 tcttggagca aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt
    14641 agtttgaaaa atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa
    14701 ataataaaaa aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat
    14761 ggcacacatt cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg
    14821 gtacatgcaa agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg
    14881 aagcgacttc cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg
    14941 tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat
    15001 cggtggttct tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa
    15061 tttattagat agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag
    15121 ctcaacatat attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt
    15181 tatttcaaaa tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct
    15241 tcttgttaag aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa
    15301 agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc
    15361 tgatgacatc ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt
    15421 tagtggtgca gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc
    15481 aagcgacaaa ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta
    15541 ccgtaaaggc aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc
    15601 agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc
    15661 ttcaagcgca aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg
    15721 tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc
    15781 tgaacaagca gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt
    15841 aaatacaaaa gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt
    15901 gctagtcaaa attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga
    15961 aattgctgta ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt
    16021 agaagcttac aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc
    16081 taaagaattg aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg
    16141 atagaaacat gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg
    16201 catcgtttat tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat
    16261 agaaatcgat tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct
    16321 gacttcgaag gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca
    16381 cttcgagtgc ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca
    16441 gatgagggta ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct
    16501 ttcaactgca agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta
    16561 atgacatcga aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat
    16621 ttaaaacctc ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt
    16681 ggagctttga ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa
    16741 cctttccatc ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg
    16801 aaagatctcc ttttttattt tta
//
LOCUS       DAFEZB010000061        16527 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFEZB010000061
VERSION     DAFEZB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.061"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(141..416)
                     /locus_tag="LP859_002088"
     CDS             complement(141..416)
                     /locus_tag="LP859_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL3030019.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="LP859_002089"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="LP859_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL3030020.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LP859_002090"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LP859_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL3030021.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="LP859_002091"
     CDS             complement(3515..4195)
                     /locus_tag="LP859_002091"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL3030022.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="LP859_002092"
     CDS             complement(4244..4879)
                     /locus_tag="LP859_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3030023.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="LP859_002093"
     CDS             complement(4945..6348)
                     /locus_tag="LP859_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL3030024.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="LP859_002094"
     CDS             complement(6335..7366)
                     /locus_tag="LP859_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030025.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="LP859_002095"
     CDS             7397..7618
                     /locus_tag="LP859_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL3030026.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="LP859_002096"
     CDS             complement(7783..8520)
                     /locus_tag="LP859_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL3030027.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="LP859_002097"
     CDS             complement(8673..10013)
                     /locus_tag="LP859_002097"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL3030028.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="LP859_002098"
     CDS             complement(10250..11773)
                     /locus_tag="LP859_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL3030029.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="LP859_002099"
     CDS             complement(12151..12762)
                     /locus_tag="LP859_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3030030.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="LP859_002100"
     CDS             complement(13092..13808)
                     /locus_tag="LP859_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL3030031.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LP859_002101"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LP859_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL3030032.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="LP859_002102"
     CDS             complement(14367..15164)
                     /locus_tag="LP859_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL3030033.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="LP859_002103"
     CDS             complement(15134..15505)
                     /locus_tag="LP859_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL3030034.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LP859_002104"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LP859_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL3030035.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5507 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DAFEZB010000062        15881 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFEZB010000062
VERSION     DAFEZB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.062"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(57..1367)
                     /locus_tag="LP859_002105"
     CDS             complement(57..1367)
                     /locus_tag="LP859_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL3030036.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1379..2797)
                     /locus_tag="LP859_002106"
     CDS             complement(1379..2797)
                     /locus_tag="LP859_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3030037.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3037..4605)
                     /locus_tag="LP859_002107"
     CDS             complement(3037..4605)
                     /locus_tag="LP859_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL3030038.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4609..5019)
                     /locus_tag="LP859_002108"
     CDS             complement(4609..5019)
                     /locus_tag="LP859_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL3030039.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5016..5939)
                     /locus_tag="LP859_002109"
     CDS             complement(5016..5939)
                     /locus_tag="LP859_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL3030040.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5936..6814)
                     /locus_tag="LP859_002110"
     CDS             complement(5936..6814)
                     /locus_tag="LP859_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL3030041.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6801..8138)
                     /locus_tag="LP859_002111"
     CDS             complement(6801..8138)
                     /locus_tag="LP859_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL3030042.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8141..9244)
                     /locus_tag="LP859_002112"
     CDS             complement(8141..9244)
                     /locus_tag="LP859_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3030043.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9318..10826)
                     /locus_tag="LP859_002113"
     CDS             complement(9318..10826)
                     /locus_tag="LP859_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3030044.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10792..11484)
                     /locus_tag="LP859_002114"
     CDS             complement(10792..11484)
                     /locus_tag="LP859_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL3030045.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11506..12480)
                     /locus_tag="LP859_002115"
     CDS             complement(11506..12480)
                     /locus_tag="LP859_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3030046.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12780..13652
                     /locus_tag="LP859_002116"
     CDS             12780..13652
                     /locus_tag="LP859_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3030047.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14110..15810)
                     /locus_tag="LP859_002117"
     CDS             complement(14110..15810)
                     /locus_tag="LP859_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030048.1"
                     /translation="MFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQ
                     TVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRN
                     LRIGLVAVSLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVS
                     RIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTAL
                     VQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEA
                     SKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSS
                     IIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLS
                     REVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPIT
                     ECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKF
                     GDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL
                     "
BASE COUNT         4678 a         3348 c         2599 g         5256 t
ORIGIN      
        1 ttaaaaaaaa taaaggtcaa gctaaaatat tatttaagct tgaccttatt ataatttcaa
       61 cgattgtcaa aagtattgtt tgcagctgtg ttgccagttt ctatagataa atcacagctt
      121 ttgaaacata agttttgtac tgcttcgatt cgcccgaatt cttttgcagt tatttggcaa
      181 tgagaaaagc agacatcttc tataggcttt tcaggaaatg ctactaaatc aaaagctcgg
      241 gaaggaagtg agtaacctgg agaaaggcat gcctgtacgt ttttgatgtg tatgtctttg
      301 actttcgtca tttgctttga ttcaggtatt tcttcagcca ctgcttgcca actttctggc
      361 atttgagcaa ggtcattgct attttttcta ttgtattcat tatgccagtc cattatccaa
      421 gaaaaaggaa attgtacatc aatcatattc agattttcta ccctgatatt ttcgatcact
      481 ccccctcttt cttttgaaga tttcattcga ataccacagt ctgactgaaa aaaatcgata
      541 tcgtgaatat atacgtcact aactccagca ctaacttcgc taccaatagt tacaccgtat
      601 cctgaacgga ttttacatct agcaacctcg attcttgatg attttcgatt gactcttgca
      661 ccattgccat ctctgccaga cttaatagca atacaatcat caccacaggc caaatcacat
      721 tcatagacac gcacatttgt tgaagaatca atatcaatcc catctgtact tggtccatca
      781 ttgttgcgga tgattacttt ttctactaat acatcgttag aataagtgat ttgcaagttc
      841 caaaaacctg atttctgaaa aatcaaatca cgaacaacaa catgttctgc atgatacagt
      901 aaacaagcgc gtggacgttt aatagcataa tccgcaatcc aacgaagtcc cttttgatcg
      961 tattcagctc ttgtcccgct tttttgatct tttccccaat acagctccca ccaatggtct
     1021 ccttgaccgt cgattattcc ttttccttct ataagaatat ctttcgccga gagaacattc
     1081 aaaatcgctg ctggccattt catttctacg ccggctactc gtgtatcgat ttctggaaat
     1141 gcttcgatat ctgttctccc cttgaggact gcaccttctt caaagataag atgactctta
     1201 tcttttaaaa aaagggctcc gactaaataa atgccttttg gaataaatac aggtaagtgt
     1261 tctttcgcac cttgatcgat tgctttttgc agtattattg tatcatcttg ttgagtgtcg
     1321 tttgttttaa agttcaaagc attgataatc cctttttttg tccgcaatac gtttctccct
     1381 atctttcatt gatttttttc aattcttcac caataaatac agtttttgaa cgattcagtg
     1441 ggtaacctac tagcaaaatg attgcggata caaataagca gaatgctggt aacaagtttg
     1501 ccatcgtata aatcctttct tgtactaaaa ctgattggat ggctccacct gttgtagacg
     1561 attggtaacc aatgagtgcc agcataaatc cgcctaccgc tcctgcacat gcttgtccta
     1621 cttttctcgc aaaagaattt actccatata ctgttccatc ttctcttaag cctgtcttat
     1681 attgatgata atcaatcacg tcagtaatga atgcccagac catcaaattg aataaaccag
     1741 atcctaaagt agcgataaat aaaagaatca gatataccca tgcatttgtt atatgaagga
     1801 aatacatcag caagtacata catgaagaag caagtaacgc aaatacgctt gcttcctttt
     1861 taccaaataa ttttgtcagc tttgtagcaa atggtgctaa aactactact gttccgtaag
     1921 taaataaaag agcaacagac attgcttgtt tattatggaa gtaatcattg aataaataag
     1981 tcatattcgt cccagctaaa atctgattga tcacaatgaa aatgtcaaca atgactaaaa
     2041 taatcagtgc tcgattagct aacaaacctt ttaccaactg agaagcagat actttttcgt
     2101 ttttgacaac tcttactctt tcagtagtca atttacaagt taacatatat gcgacaaatg
     2161 ctattgcagc acatccgatt gaaatccaaa agaaatgatt ccctgaaacg acttgttgtc
     2221 cactcttgtt atgggtatac attaaaatcg gaatgaagaa tcctgtagtt gatccgccaa
     2281 tcgcagaacc aatgcttcgg aacgtggaca acgaggtgcg gtcattagga tttgagctaa
     2341 ttgctgctgc cattgctcca tacgggatat tgattgttga gagaaaaatc ccataaacaa
     2401 tgtatgaaac aaaaatataa gcaatcttca ccgtcattgg aagatgttgg acgaatggca
     2461 aaaacaaaat gacagtcact aagcaaaatg gatacttcat ttttctgatc caaggaataa
     2521 atcggccagt ttctgttaac tctgagacat cacataatct tccaaccatc acatcagcaa
     2581 acgcatccaa aattctcgaa atcaagaata acgtacctac gattgcccct gaaacgccaa
     2641 gaacattcgt ataataaatc atcaagaaac tattgactaa acctaaaata aaacagttac
     2701 ctagatcacc gaacatgtag cctatcttat cggaaatccc aaattccttt ttttcttttt
     2761 ttattgtatt tgctgtaaca gcattttgtg aaagcatttg aattcctcct acctcttcta
     2821 gtaaacgttt tcaaaattat aactcttttt ttcttgtttt tatattcaca atgttggctt
     2881 tttattgcat tatcttgaca gataaaaaac aaaaaataat ctaaaaacat tgatgcataa
     2941 gattgataac tttttttaca taacaatata tacaaacaaa aaaattcact aactagtgaa
     3001 aataagttag tgaacctcat ttattaaaga attaaattac agatgatctt ttccatcata
     3061 cttgaaaaac tcaaaatcag cataacttcc tcttttttta tttagatcat gcactgctat
     3121 cccgataaaa tttccagtaa accctcctga gagtatacac atgttttttt gcggacctac
     3181 tttcttccat tcttcagttg atacgtcttt caaatgaaac tgtgctgtct ctttctttac
     3241 agtgactttc aaaaaagtct tcttttctgt gagtggaatc gtccaatctt ctatttgtgc
     3301 tactccgttt tctgattcta tcatacgtag gcattttccc ttgtcctcat tccaagtaac
     3361 ataacaataa atataattgg attcatttaa gtacagtacc agtccagcca tctgattaaa
     3421 attattaggc atgtattcaa atgctgtctc cgcatcaaaa gtaaaatccg tttgccgaat
     3481 agctaacaaa tgatgttcga aggtagattg aagagattca ccggaaatca gacgtaaaaa
     3541 acctggtcta cattttgtat cacaccaatc agcatttggc aaaatgcgtc tggcattcca
     3601 gttcttcttt agctctcctt caaatgtatc cataaactga tgatcttttt cttgttgtac
     3661 ctcttcattt gtctcaatca ttatttcttc catcggagtt gtctggctat ctttaagtct
     3721 taaccaatcg tcggacgtcc aatacacttc ttgaatagcg gtttctcttc ccaaaatagc
     3781 cgcaccgttt aatggtctgg tacaaagata tgtcatgtac catcttcctg acggggtttg
     3841 tactaagctc ccatgtcctg tacattgcaa tgtagaagca ggtttgtcac tagctgtcag
     3901 cataggaaaa ccaggatcaa gctcgtaagg accagtaata ttctttgctc ggcaaactgt
     3961 aactgaatgt cctcttcctg taccgccttc tgccgtcatc aaataataat acccgttacg
     4021 aaaatacaaa tgtggtgcct ccgttttcgc taattctgta cctgcaaata ttttatagat
     4081 gggaccatca agcttcttct tctctgcatc atattcttgt aatacgatac cgctggattt
     4141 attcggtgtg tccagacgat aatcccatag ttcattgaca agccattttt ttcccgtttc
     4201 aagatcatgg aacaaagaag gatcaaagcc gttactattt aaataaattg gttcggacca
     4261 aggacctgtg atctcagatg cagtgatcaa ataattgtgg acatctttaa acggacggcc
     4321 tgcacttttg acatctgtgt aaacaagata aaacaatcca tcagcataac tgatctgtgg
     4381 cgcccatata cttccatttg taggatttcc ttgcaaagat acttgagtgg tcaaaatatc
     4441 cgttgtatgt gaccagttga ctaaattttt cgactcatat actcggattc ctggaagcca
     4501 ttcaaaagag gagacaataa tgtaataccg ctctttcact cttaatatat taggatctgg
     4561 attgaatcct cttaaaatag gatttttcac tttgatagtc tgcatgattc aggctccttt
     4621 tctcattctt actcgatata aactgaatgc cccagctgcg aaataaaaaa atgcaaaagg
     4681 aaacgcaata aactgagtgc caatcaacaa tatgaatcct gttataaaat acaaaccagt
     4741 ggcagttgtt aagtattttc ctttagcagt caaaaacaat gctactgcta aaaaagcaag
     4801 tgaaataaac aacacaacgg caaacagacc agtcatgtcc tgtaataatc caaatgtatt
     4861 ttctaaagac aaagttgaat gatctacagc aagcgactga taactttttt taaactctga
     4921 cgcagacata ttacttaaag taactgctaa cccgccaaag aaaatcaaag atagactgcc
     4981 accgaccatt cctagcaata gttcccattt tcttttcatt tctgaacccc ctgcttttct
     5041 aaatgtatcc attcaggtct tttgatcgat gtttcataat tgttattacg ttcagagaat
     5101 ttgacagtct gttcattttt cttgtcatcc caattatccc aaccagttgg gtgaacatgc
     5161 ccacctatct tacacgcttc aaataagaca tttgcatacg gacgccacgg tcttcctaaa
     5221 tagacgtttc tagtatcagg ttctgcagtc aaggtacagc tttcaaaacg ataccctgtt
     5281 ttttgatttt tcggcgtaga tgctgctgtg atgtatccag gatttttact ggctaagcta
     5341 tggatatgac actgatgaaa aacggcatct gccccaccaa atatgaaatc caccgttcct
     5401 tcaatccagc aatattcata ggtctgacga caatactcag gattggcaga agccggtgta
     5461 caaaaaggag taccatcttt ttgcaaatca ggcaacggac ccgtgcataa ggtatcctga
     5521 taaccagata gtcgacagtt ttttaaagat acgcgatctc ctgaattgaa taaagcaaca
     5581 gcctgtccta ccttttctcc tggacctgct gtattttcga tgtgaagatt ttctaatacg
     5641 acattcgatc cttctaagaa aaaagtagca cttcggaaag tccctaactg gctaccgtca
     5701 agatactcct gttttgcaaa acgattaccg gagataacta ctgttcctgc tcccacaagt
     5761 ttaaaattgg ataatctgct ttctatatat tcatcatatc ttccttcttt tactatcaag
     5821 acctttggat ggctatccgg caggcctgcc aaataatcga ttccttcttg gatagtttca
     5881 aaatccgtcg gggtatctat agcattcttt ccaattatca gtttgtcttt catcctcata
     5941 aatgctccct tatgaattgt ttccaaatat ttctcatttc ttgtgtttcc atatgaccgc
     6001 ctgttaaaga aaaagaagaa aagttttctg gactacccat tgattgatac tttttgtcca
     6061 gcccttttct cagcttcagt acgccttcgt ccaagcacat cgtatcgttt tttccaacta
     6121 agctaagacg agggcgcgga gcgatttttt cttggattgc taacgttgta taagctgtca
     6181 atagatttgg cacatagtaa taaaacccat gatgatctag gcggcgatgt agcaaaagta
     6241 tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt gacacgagaa tccaatgctg
     6301 tcacccacca gcttaataag cctcccatag aaagaccaat cgttgcgagc cgttgagtat
     6361 ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat cacatcataa atacgcattc
     6421 cccataacgt ttttcctgtt aataaaaatt gtttgaacaa ctcgctttca gaaatacctc
     6481 cgcgttcttc aaatccccaa gcatcaatcg accaaacagc atatcccatt cctgtcagta
     6541 cttctgcaaa agatggagaa gctaagtaag gagcaccatg gatcaattca cttttcccga
     6601 ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgg cggaaacggt ccttcttttt
     6661 ctggataagc atagagggct ggtactgttt ccaattcatt taagtacaat tgatagtatt
     6721 caagcataaa accgttttgt ttttctcggt ataatcgttt tccactaggg gtatgtctgc
     6781 tagatggagc acctaaaaaa tcattcaact gcataagcaa agtttttacg aacaaccagt
     6841 tcatccatat tttctgattg atttgattgg aatttctcaa tactgatttc tttagtattt
     6901 tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca tctggttaaa ggaaacctta
     6961 cccgcaaagc caatataaaa tccttgtctg ctcatttcct ctattcctgt catcatagca
     7021 ggaacaccgg gaactgcatc ttgggccatc gagacatcga tattgtcaaa tgtgatttct
     7081 tgtatgaatt gttccgctaa tccataaata aaacctgcag aagcatggac attccttgcc
     7141 gtgatattcg aaaaatggat ccttctgaat gctggtgtcc gttcatctat cggataagcc
     7201 tttttttccc atacatacgg ttctttccct cgcggaccac agaaataata aagattcagt
     7261 ataaatggac acatcacatt atccatgact atattggata ctcgaatatc ttcaacgatc
     7321 ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat cagtttcttg gaagatacaa
     7381 tttgaaatgg taatattacg aatactacca ctcatttcac ttcctaatac gacaccgcca
     7441 tgtccatgga ccatcgtgca atttgtgatc gtgatatttt cgcaagcgat cctttcgtac
     7501 gtatcttcag tccctgcctt gattgcaata cagtcatctc ccacgtcaat atggcaatta
     7561 ctaatacgca catttttgca tgattctgga tcaattccgt ctgtatttgg tgaatccgct
     7621 gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc aaagaatcgg gttgatcgtc
     7681 caacttggtg actggatcaa tttaatatct ttcacagtaa ttcgatgaca gttatggaaa
     7741 ctcatcaatt taggacgagg ataagcgaga ttatctggtt catttctaaa agtatgccac
     7801 catttttttc catttccatc taatgtaccg aaaccagtga cagagatgtt ctctacattt
     7861 tgagcataga tacaggaagc ataaactttt cgatggactc cttcccaacg tgaatgaaca
     7921 acaggataat cttttggatc gtcagagaat ttcagaaccg ctcctgcgct taaatgaagt
     7981 tcgacattgc tttttaaaaa taaagctcct gttagaaatt cacctgctgg aacaactacg
     8041 gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt gtattgcttc agtattcaat
     8101 tcatcaattg atgcgccaaa tgtcaaaata tcatacatat ttataatgcc tccacttcaa
     8161 tcaatgcttg caagaatgca ccaacgcctt tttgatcatt acaaataatt ggttcactga
     8221 tgtagtaagc atatgaacca tctcgctgat ctgctccgcc taaccctgct acttgacagt
     8281 ttttattcaa gttcagccag ccttcttttg tttccagaac aaattcatca agtaatcctt
     8341 gatgtgattt cttcacttgt ggaagccatt cttctttgtt cagcagtccc aaacgtattc
     8401 ctttagccat tgcgtaaaca aacatactac tgccggaagc ttccagataa tttcctttcc
     8461 gttttgtttc tgttgttact tgataccatg tcccgctgtt ttcttcttga tatggaagta
     8521 atgcagccag tgtcttttct agaatttctt tcagcaaaga acaatctgtt tcttcatgaa
     8581 gtaattcata ggtatctgct gcagccatca aataccagcc aatcgatcgg ctccaaaaat
     8641 gatgggatag acctgttttt ggatcagccc atggttggac cttcttttca tcccatgcat
     8701 gaatcagcaa atttgttttt ggatcgaata gatgtttata actgatttca aactgtctca
     8761 aaacatcttg gtaatctttt tcttcactaa aattctggat aaattcagca taaaaaggag
     8821 aacccatata taagccatcc aaccaaattt gataaggata aatttcttta tgccaaaaag
     8881 ctccttcgga tgtgcgtgga tggtttgcca actgctggga aaggagttct gctgcttttt
     8941 tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa aagctttcct gtatttacat
     9001 ggtcgatatt gtattcttca acgctgtagc cacggatcgt tccatcttct tgcacgaaat
     9061 agtcgatatt gtcttttatg aaatcaaaat aaattttttc ttgggttcgt tcccaaagta
     9121 attggaaccc tttaagtacg acgccatagt cataagacca ttttccttta taccactttt
     9181 cttcatataa gcgaggtgtt ctttccatga tactttttgc taatttcttt gaccagtttt
     9241 ccattatttg ctctccttta aaaattaaaa taggatttgg gacaaatacc aagaagtatt
     9301 tgtcccaatc gtctatatta ctgtgccgct gctttgtatg cttcctcgta ctcttgtgtg
     9361 attcgatcgc ctccttgtgc tttccagtct tctacagctt ttttgaatcc atcttcgtca
     9421 agctgtccca tgatgtattg caccgtagca tcaatgacga ttttttcaat ttctgatcct
     9481 acttcattgt tcgtcggtga atccaatggc acagaaggat ccattacagc aaattcttca
     9541 ttttctttga tcaattgatt agagaattct ttttcagggt ctgtatcttt gaaagagaac
     9601 gtcacttcac taggacgaga gcttgataac ggttgcacct cttgttgcca aagatccata
     9661 tcgataaatt cgatagaacc atcatcattc aattcataat gcgttccttc gataccgcca
     9721 gtcatcaatt tatacacatc ttcttctaaa agatcatcaa caaattgaag taatttcttc
     9781 aattgtgctt cgtcttttac ttgagatttg ggaaatgcta gcaagccgcc aataccattt
     9841 ccttctgacc aaacatgata accgccgcct tcaccatctg agatcttgtt tacaggcact
     9901 aattccatat catcttgaat accttgaccc atcgtcttca gattcttgat atccaccatt
     9961 ccagtgtaga ttccagttct tccttgtgca aattgttctt gctgatcagt cttttgggta
    10021 actgcaaagt cttgtgccaa atagccattc tcaaatagat ctcttgaaaa attcaaagct
    10081 ttcatatact cttctgtatc aaattctgga gtgaatttgc cattatcgtc tacagcccag
    10141 ccatttggtg ctccaaaata agaagtgaaa agtttaaagc tggtataacg aatatcacta
    10201 ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat ttccatcagg atcgttttct
    10261 gtaaattgtt tagctacttc ataaagctca tccatggtag ttggaacatc tagtcctaga
    10321 ttatccaacc agtcttttcg aatgactaag ccagcacgag cgtagtcttt ttgtatcggt
    10381 acgccgtaca aggcaccgtc gatactcgca gcttcgattc tatcttcaga aatttgactt
    10441 aaatttttat agtcttttaa atattctgat acatcccaga atacgcctga ttttaacgca
    10501 ttgcggacag aagagttttt caacatcgca atttgaagtg taacgatatc tcctagttca
    10561 tcagaagcca atgcagttgt taatctttct tcttttgaag catcaggaat ccagttgaat
    10621 gtcagttccg tatcggtttt ttcttcgatc aagttcacaa tcgtgtctgt aggcggtgaa
    10681 gcatgatgca aaatatttaa ccaagtgatt ttagcttttc catcttctgt ctctgaagaa
    10741 ctgttacttg aactgctgcc gcatgcggca agtgctagaa ttcctgcaaa actaagacct
    10801 aattttacga gcgttgcttt tttcataaat tttctccttc actgttttaa taatattttt
    10861 gttttcttct gttttttctg tatatgagtt cttccattcc aaatggcaaa atatagaatt
    10921 tgctgtccac gtgactgcgg aaaagaagag actgccaccg ataaaggtta aaacacctgg
    10981 cattgtttgg atgatcatca gataaatccc taccatactt aatatcaaca ctaatgagta
    11041 atgaagatgt gagaatccga ataaaaatgc cattttcatt ttgaaaaaat atcctttcca
    11101 atcataatga gccatgattg ggaaaatata gagtgctgag agcacatata tgaacgcagc
    11161 aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa gaaagatcca caagtaaaac
    11221 aatcccgatg gcgccatata tccagctcat cagcaaggtt tcacgatagt tttctttaaa
    11281 atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt gagcgcagcc attttctaat
    11341 cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa actcccaaac ctgcgatgac
    11401 taaaccgccc cacagaaaat tcaaataaac caactgtaaa atacgattta gccttccgtt
    11461 tattttatct aatgactgca gcatatcttt ccctctcttt ctatcttagt aaacgccgtc
    11521 ttctcctagc tttttggcaa tcttattagc taaaattacc ataaacaatc ctacgacccc
    11581 tttaaacaat cccattgctg tactgaaact taactgtcca ttcttcaatc cagctgtata
    11641 aatataggtg tcgaaaattt ctgcaacgct tcgattcaat gaattcaata acaagtacat
    11701 atgctcgaac cctaagtcta atgtctgacc gatttttaga atcagcaagg taatgattac
    11761 tggacgaatt gccggcaatg taacatgcca tgttttgcgt aagcgtcctg ctccatccat
    11821 ttctgcagct tcatacaact gtgtatcgac tgcagttatc gctgaaaggt agatgatcgt
    11881 agaccagccc aactctttcc agatcacttg tccgatatat actgtgcgtg tccattcagg
    11941 ggatgttaga aaactgattt tttcaaatcc cattgttgca aggaattcat tcaatacccc
    12001 tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg attacccaag acatgaagtg
    12061 agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt ttgttcgtta cttcatttaa
    12121 tagtaaagac aaaatgattg gcatcggaaa aaacacgaag atatttaatc cgaacaagat
    12181 caacgtattt cttaataatg taaagaatgt cggttctgta aacaaacgaa tgaaatgttt
    12241 gaaaccgacc caaggacttc ccattattcc taaaaatggt tgataatctt gaaaagcaat
    12301 aacgagtccc cccataggaa gatacctaaa aatcaagaaa taaaggactc ccggcagaat
    12361 catcacatat aaaaatttat tcgttttgat tcctgcaatt attttttttc gtcttctgag
    12421 cttttcttct tttttcgata gcgtcagttg aaatggttct tgtttcttta cactttccat
    12481 aagcccctcc ttagttttgt gaatgtttgc tgcttatgac ttcatgatag ataactatct
    12541 gaattacgta tataatcatt tttaccgtaa gtaaaaacgc ttacatagca atgatttttg
    12601 tgctatacaa aacgataaag tgtatttttt cacaaataaa ctacgcatag caaaccttct
    12661 atttttttct atctgacact tagcatagga aatgattata tacgtaatct ttcaccgatg
    12721 ataaattgaa catatagaaa cagaaacatt taatttattt tgaggaggag cctatctata
    12781 tgaaaaagaa agtctctata ggagaaagaa gttttacact tttcaacaat acgtttctaa
    12841 ttttgctagc tttgatctgt atcgttcctt ttttaaatat catcgctaca tcttttgctt
    12901 ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc tactaccttt tctttagacg
    12961 cgtatcgcta cattctttct acgccgacca tttttagagc acttgctgtt tcgattggtg
    13021 ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc actgatggcc tacggtcttt
    13081 ccagaaagta tttgtttggt cgaggtttcg tcaactttct tgttgtattt tctatgttgt
    13141 tcagcggggg gatgattcca acgttcttag ttgttcgttc tctagggctg gtcaattctt
    13201 actgggccat gatcctgcct gtggctgtta atgcaatgaa tatgatcatt atgagaaact
    13261 ttttccaagc cttgccggat agtttggaag aatcagcaaa aatggatggt tgcacagatt
    13321 ttggtgtatt tttcaaaatt atgctgccat tagcgttacc gtctattgct actatctcgc
    13381 ttttttatgc tgttacttac tggaacactt atatgactgc tattttgtat atcaacgatt
    13441 catctaaatg gccgatacag attcttttac gccaaatcgt cattgtatca agtgggatgc
    13501 aagcagaaag tagtgccgtt gatgtgattc caccagcaca aacagttaaa atggctgtta
    13561 tcgttattgc aacagtgcct atgctgattg tttatccttt tgttcagaaa tattttgtca
    13621 aaggagcact cgtaggttct gtcaaagggt gattatgtac tgaacgcaaa atggtagttg
    13681 tgtcagaagt ggtcagcttc aagaaataag aaggcattta cgaaaattgc ttcttaaatt
    13741 tttgtgaaat ttcggcttat ttttcgaaga atctactcct ggaacaccgt gtatcagagg
    13801 ttgtgaaaga gacgttcagc cttgagcgat gataaaaact ctcacaaaca ttgcgacgag
    13861 taacccacag gagcaataag aagcgatttt gaaaaatagc ttcccatatt tcacaaaatt
    13921 atgccttatt gtcgatagcc tgtctcttga acgccattta ttcgtaaaaa agaactgtga
    13981 caagactttt gtcacagttc tttttgcgtg cttagccttg aaatttaact atccataaat
    14041 acaaatacta gggttattct aacttctttt attgcttgaa tcagtccaca agaaaaacag
    14101 cctcttgatt cacaaatatt tttttcgata ttctcccggc gtaatcgatt ccttcttttt
    14161 aaagaaacga ataaaatttt gcggatttcg atattgcaat ctttctgcaa tctcttttac
    14221 ggagaggtta gtctctttca accatttttt tgctacctct aaacgatgat tttgaacaaa
    14281 atcaccaaat ttttctcctg tttccttttt aaatacatta cttaaatagt tagggttata
    14341 atgcaaccta tcagcgatca catctaaaga cagatcacga tcatattctg tgtagacgat
    14401 atgcataata ttctccgaca aatttttaaa ttcatgatca gtgattgctt gagagcattc
    14461 tgtgattggc agaactaatt gttctaaaac aagtttttct agttctttgg gatgataatt
    14521 gttcagtgct tttacataaa tttgtttcat gttatcaaag ttttttgaat ctactcccaa
    14581 taactgtcct aattgaacga gttcattgat caagcggacc gaaactactt cccgactcaa
    14641 aggattttta ttttgtgcaa acaatgcatc aatcaattca tgtactaaat tcttcacttc
    14701 ctgatttcca gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg gatatctaat
    14761 aagtgttttt tcatgagcag caggaacgat ttcctgataa aaaataattg aacttggacc
    14821 agtatttaca cgataataca gggcttcttt tgcccgatca accgattgct tgctttcatt
    14881 cagcgtttcg aaccggttac tgatgcctat actgatagtt aaattcaagt attcttttac
    14941 acgtttttga atgagtcggc aatactccat gacttttttg cttgcttctg catcatcttt
    15001 atccattcga taaatagtgg cttgcatttc ttcattcaaa acgatcggca gcattcgatt
    15061 ttcctttggc acaatttccg tgatcatgtt gttgatcgct aaaagtagca aatcacgctc
    15121 cccaccgtga ctatcttcta acagatcgat ttgaaccaaa gctgtataat agcagtcatt
    15181 ttgagaacta taaccaaatt gctgcaaccg ctgatctagt tctcgccttt ctactcgatt
    15241 tctaaataaa ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa ctaagtgtgc
    15301 acttaacgct tctttttctc ctatgatccg actgactgat tgagcgacca aatctagttc
    15361 tttcccactg aatccttttg atttaagatt cagacgttcc tgtatttgag aaatcggacg
    15421 ggcaaatctg tccgaaaata agtaggaaag aagaccaatg agtcctacca gtgttaaaga
    15481 cactgcaact agcccgattc tcagattgcg gatcattgcg ccaattgcac tagagtcaac
    15541 ttctaatctg taaatccaat tattataatc tgatttcagt actatatacg ttttatcttt
    15601 ttctcttatc actttcagct gattgtaatc tagatccacg tccgttaata cagttggttg
    15661 ctctttattt tttactgtct gagtagtaaa aaggttgcca acttcgtttt caatagaaag
    15721 tatgtcttct ccactttcac caacaacttg ttctattgta tgtttctcta tcgtagcaat
    15781 tcccaatgca tatttttctt tttcaaacat tggcaaagaa atcaccattt cgattccttt
    15841 gtttttcttt tgccaaaata aattattttt gttatttatg t
//
LOCUS       DAFEZB010000063        15859 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFEZB010000063
VERSION     DAFEZB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.063"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(12..482)
                     /locus_tag="LP859_002118"
     CDS             complement(12..482)
                     /locus_tag="LP859_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL3030049.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="LP859_002119"
     CDS             complement(641..1099)
                     /locus_tag="LP859_002119"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL3030050.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2414)
                     /locus_tag="LP859_002120"
                     /pseudo
     CDS             complement(1270..2414)
                     /locus_tag="LP859_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2427..3344)
                     /locus_tag="LP859_002121"
     CDS             complement(2427..3344)
                     /locus_tag="LP859_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL3030051.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3772..4335
                     /gene="efp"
                     /locus_tag="LP859_002122"
     CDS             3772..4335
                     /gene="efp"
                     /locus_tag="LP859_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL3030052.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4436..4606
                     /locus_tag="LP859_002123"
     CDS             4436..4606
                     /locus_tag="LP859_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030053.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4821..6080
                     /gene="lysA"
                     /locus_tag="LP859_002124"
     CDS             4821..6080
                     /gene="lysA"
                     /locus_tag="LP859_002124"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL3030054.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6067..6867
                     /gene="dapF"
                     /locus_tag="LP859_002125"
     CDS             6067..6867
                     /gene="dapF"
                     /locus_tag="LP859_002125"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL3030055.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6870..7751
                     /locus_tag="LP859_002126"
     CDS             6870..7751
                     /locus_tag="LP859_002126"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL3030056.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7748..8464
                     /locus_tag="LP859_002127"
     CDS             7748..8464
                     /locus_tag="LP859_002127"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL3030057.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8470..9165
                     /gene="dapD"
                     /locus_tag="LP859_002128"
     CDS             8470..9165
                     /gene="dapD"
                     /locus_tag="LP859_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL3030058.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9181..10182
                     /locus_tag="LP859_002129"
     CDS             9181..10182
                     /locus_tag="LP859_002129"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL3030059.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10179..11540
                     /locus_tag="LP859_002130"
     CDS             10179..11540
                     /locus_tag="LP859_002130"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL3030060.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11500..12651
                     /locus_tag="LP859_002131"
     CDS             11500..12651
                     /locus_tag="LP859_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL3030061.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12626..13795
                     /locus_tag="LP859_002132"
     CDS             12626..13795
                     /locus_tag="LP859_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3030062.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13970..15349
                     /locus_tag="LP859_002133"
     CDS             13970..15349
                     /locus_tag="LP859_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3030063.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15630..15859
                     /locus_tag="LP859_002134"
                     /pseudo
     CDS             15630..15859
                     /locus_tag="LP859_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5185 a         2797 c         3378 g         4499 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatctttt ggttagaagt cactaaacca
     1801 gctactacat cgctgtgtcc gcctagatat ttggtaccgc tatggacaac gatatcagaa
     1861 ccgagatcta aaggtctttg aaaataagga gtagcaaatg tattgtcagc aataactatc
     1921 aatccatgtt gatgagccag agtggcactt ttttctaaat cagtaatctt tagaagcgga
     1981 ttgctaggtg tttcaagata aagtgctttt gtattgggtt tgatggattg ttctatttta
     2041 tcaagattac tcgtatctat gatcgtgtac tcgagaccat ttttagtaag aactttgtca
     2101 aatagacgga atgttcctcc atagacatca tctcctaata agatatgatc gccagcttga
     2161 aaaagagaaa aaacggcatg aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca
     2221 ccgccttcca aatcagcaat cagttcttcc aatgcaaaac gagtaggatt accagatctt
     2281 gaatattcat attgtttagg ctggccgacg ccatcttgcc gataagtgga tgtttgataa
     2341 ataggtacac tgacagctcc tgttgtggga tcttcactga tgcctccgtg gatcaatttt
     2401 gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt tttttgataa atagcggtct
     2461 gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg gtaaacgacg aatttctctt
     2521 aatgctgctg caaaagccgc accactcgaa cttccaacta atagtccttg ttctcgtgct
     2581 aactgcctag tatagttaaa cccttctaca tcagagattg tctcgatctg attgattgaa
     2641 aggggagaaa gaaacggcgg aatgaattct acaccgatac cctcaatttc gtgaggggct
     2701 ggatcaccac catttaacac agagccctct ggttctacac cgattattcg aatgtcggga
     2761 tatttttctt tcaaaaaggt agaagttcca gcaaaggttc caccactccc aattcctgct
     2821 acaaaactat ggatattttc tttgatttca tgaaagattt ctggccctaa cgtatgatag
     2881 taagcagcag gattatcttt attttcaaat tgaagaggca gatagctgtt tgatatctcc
     2941 tctgcaagtt ttttactttt gctgatggca ccgatgattc cttgctcact aggagaatgg
     3001 atgatctttg ctcccaatgc tttcatcaat tgttgttttt ctaagctaaa cttctctgga
     3061 acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca gagcgatccc aattcctgta
     3121 tttccggcag ttggttcgat gatcgtcgtt ttttcattga tttttccttg ggcaaatcct
     3181 tctttgatca aataactgcc caatctatct ttgatacttc ctccaggatt taaatattct
     3241 agttttgcat aaatagcaga acctttaggc atagaatagt gctgattgtc aaagcgataa
     3301 agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa
     3361 aagataataa aaaaaggagc tgactattaa atcagctcct tgaaagacat agagaaaaat
     3421 tgagtagata aaaagataag ttacctggta ctcattctat gaagggcaat taatagacat
     3481 gacaaatagt atgctgcttt tatgcaccat acaacagcaa cgattcaatt gtttttggtt
     3541 gctgactgtc atgtgtatcc ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa
     3601 taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt
     3661 tttctgacaa ataggcatga gaccttaaaa gatagagaat actctagttt tctggccggt
     3721 tgtctgatac aataaagttg attatgataa agaaaagaag gagcaaaaat aatgatctca
     3781 gcaagtgatt taagagccgg tatgactttc gtacaagacg gcaaactgat taaagttctg
     3841 gatgcaagcc accataaacc tggtaaagga aatacagtta tgcgtatgaa actgaaagat
     3901 gtccgttcag gtgcaacata cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca
     3961 catatcgaca caaaacctgt acaatatttg tacacgatgg atgatacagc tttcttcatg
     4021 gatttggaaa catatgagca atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa
     4081 tatatcctag aaaacatgga agtaaaaatc caatttttcg gtagcgaagt catcggcgtt
     4141 caattaccaa caactgtcgt attacgtgtt gtggaaacac aaccttccat caaaggtgca
     4201 acagtcactg gttcaggtaa gccagcgaca atggaaactg gcttagtagt caatgttcct
     4261 gacttcatcg aaacagatga attgttggaa atcaatacag cagaaggttc ttatgtaaaa
     4321 cgtgcgtcaa aataattcgc taacggattg ataagaattt gaaaacagag cttgacctct
     4381 tgaaaggcag atcatcggat ctgcctttca agaggttttt tgtggaatca gcaaattgaa
     4441 aaataaaaaa gtatttgatt ttatttatat tttgaaagat gaatggcaaa agctagagaa
     4501 agaaaaaact gcacttattt atgaagaaat tgaaatgatt cataaaattg aaaatatccg
     4561 aactattccg aactatattg aagcgaaaat attttttcat cactaaatag tgttgaattt
     4621 atgtttttat gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt
     4681 attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa
     4741 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
     4801 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
     4861 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
     4921 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
     4981 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
     5041 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
     5101 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
     5161 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
     5221 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
     5281 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
     5341 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
     5401 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
     5461 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
     5521 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
     5581 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
     5641 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
     5701 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     5761 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     5821 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     5881 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     5941 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     6001 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     6061 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     6121 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     6181 atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     6241 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     6301 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     6361 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     6421 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     6481 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt
     6541 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     6601 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     6661 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     6721 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     6781 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     6841 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     6901 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     6961 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     7021 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     7081 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     7141 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     7201 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     7261 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     7321 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     7381 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     7441 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     7501 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     7561 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     7621 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     7681 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     7741 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     7801 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     7861 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     7921 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     7981 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     8041 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     8101 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     8161 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     8221 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     8281 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     8341 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     8401 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     8461 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     8521 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     8581 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     8641 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     8701 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     8761 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     8821 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     8881 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     8941 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     9001 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     9061 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     9121 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     9181 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     9241 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     9301 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     9361 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     9421 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     9481 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     9541 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     9601 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     9661 gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat
     9721 ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat
     9781 acaaaagaag aacagaaaat gataaatgag aaccgtaaga ttctgcatca gcctgctttg
     9841 ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca
     9901 gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca
     9961 gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga
    10021 ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg
    10081 ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt
    10141 gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag
    10201 cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg
    10261 ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag
    10321 atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg
    10381 attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg
    10441 ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa
    10501 agataactga agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg
    10561 agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga
    10621 aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa
    10681 tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg
    10741 ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga
    10801 attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa
    10861 tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc
    10921 ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata
    10981 ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg
    11041 gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata
    11101 tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat
    11161 caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga
    11221 ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa
    11281 tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga
    11341 ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct
    11401 tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa
    11461 cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg
    11521 gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat
    11581 gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt
    11641 tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca
    11701 gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt
    11761 ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac taaacacatt
    11821 gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc
    11881 gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc
    11941 tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg
    12001 gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt
    12061 gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt
    12121 gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga
    12181 acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt
    12241 tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct
    12301 acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc
    12361 ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa
    12421 gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc
    12481 ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat
    12541 ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc
    12601 agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct
    12661 ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga
    12721 tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc
    12781 aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga
    12841 actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca
    12901 aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt
    12961 tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt
    13021 agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg
    13081 tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat
    13141 tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat
    13201 cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt
    13261 gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat
    13321 aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca
    13381 aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac
    13441 gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat
    13501 gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt
    13561 tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca
    13621 aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc
    13681 aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat
    13741 ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat
    13801 agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt
    13861 ttttaaattt agttcaaatg aacgcgtttc ttttgattta taactaatgt aatgttaagt
    13921 ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat
    13981 caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac
    14041 acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat
    14101 gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa
    14161 tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa
    14221 aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa
    14281 agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt
    14341 accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat
    14401 caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat
    14461 tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt
    14521 gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca
    14581 cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa
    14641 cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca
    14701 agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg
    14761 tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg
    14821 agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac
    14881 tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa
    14941 accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc
    15001 aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta
    15061 tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat
    15121 ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta
    15181 tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga
    15241 agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt
    15301 gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat
    15361 atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa
    15421 aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac
    15481 accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag
    15541 accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt
    15601 aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag
    15661 atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt
    15721 tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc
    15781 gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca
    15841 attcttatcg aggaaaagt
//
LOCUS       DAFEZB010000064        15313 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFEZB010000064
VERSION     DAFEZB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.064"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            278..787
                     /locus_tag="LP859_002135"
     CDS             278..787
                     /locus_tag="LP859_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3030064.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LP859_002136"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LP859_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3030065.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LP859_002137"
     CDS             2322..3302
                     /locus_tag="LP859_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3030066.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LP859_002138"
     CDS             3424..4842
                     /locus_tag="LP859_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL3030067.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LP859_002139"
     CDS             4913..6382
                     /locus_tag="LP859_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3030068.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LP859_002140"
     CDS             6392..6859
                     /locus_tag="LP859_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3030069.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LP859_002141"
     CDS             6895..7767
                     /locus_tag="LP859_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3030070.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="LP859_002142"
     CDS             7849..10383
                     /locus_tag="LP859_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3030071.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="LP859_002143"
     CDS             10539..11312
                     /locus_tag="LP859_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3030072.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="LP859_002144"
     CDS             11305..12096
                     /locus_tag="LP859_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3030073.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="LP859_002145"
     CDS             12106..12597
                     /locus_tag="LP859_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3030074.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="LP859_002146"
     CDS             12615..13025
                     /locus_tag="LP859_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3030075.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="LP859_002147"
     CDS             13029..14429
                     /locus_tag="LP859_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL3030076.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="LP859_002148"
     CDS             14452..14826
                     /locus_tag="LP859_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3030077.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4782 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttc
//
LOCUS       DAFEZB010000065        14840 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFEZB010000065
VERSION     DAFEZB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.065"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            418..1032
                     /locus_tag="LP859_002149"
     CDS             418..1032
                     /locus_tag="LP859_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3030078.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1805..3544
                     /gene="yfcC"
                     /locus_tag="LP859_002150"
     CDS             1805..3544
                     /gene="yfcC"
                     /locus_tag="LP859_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL3030079.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3701..4885
                     /gene="iadA"
                     /locus_tag="LP859_002151"
     CDS             3701..4885
                     /gene="iadA"
                     /locus_tag="LP859_002151"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL3030080.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4996..5199
                     /locus_tag="LP859_002152"
     CDS             4996..5199
                     /locus_tag="LP859_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL3030081.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5300..5962)
                     /locus_tag="LP859_002153"
     CDS             complement(5300..5962)
                     /locus_tag="LP859_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030082.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5975..7477)
                     /locus_tag="LP859_002154"
     CDS             complement(5975..7477)
                     /locus_tag="LP859_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3030083.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8025..9704
                     /locus_tag="LP859_002155"
     CDS             8025..9704
                     /locus_tag="LP859_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL3030084.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9831..10310
                     /locus_tag="LP859_002156"
     CDS             9831..10310
                     /locus_tag="LP859_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL3030085.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10529..11209
                     /locus_tag="LP859_002157"
     CDS             10529..11209
                     /locus_tag="LP859_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL3030086.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11217..12548
                     /locus_tag="LP859_002158"
     CDS             11217..12548
                     /locus_tag="LP859_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3030087.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12563..13525
                     /locus_tag="LP859_002159"
     CDS             12563..13525
                     /locus_tag="LP859_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL3030088.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13636..13974
                     /locus_tag="LP859_002160"
     CDS             13636..13974
                     /locus_tag="LP859_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL3030089.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14053..14760
                     /locus_tag="LP859_002161"
     CDS             14053..14760
                     /locus_tag="LP859_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL3030090.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3192 g         4308 t
ORIGIN      
        1 tttttttgta ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag
       61 aaacagctga ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga
      121 ccagtcacac acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc
      181 taatttattg tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt
      241 ctgcacgttg ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt
      301 gtgtttgaaa cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt
      361 ccttagatta ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg
      421 agtttatgtt attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg
      481 ctgatgcttc aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag
      541 atatatgcta gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat
      601 cttgtaccaa gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt
      661 gcaggacagg caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt
      721 tcgaatgaag gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt
      781 catccattaa tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc
      841 agtggtttgc gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat
      901 gatgcagtct tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca
      961 acgatctttt tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca
     1021 agtggaaaat agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa
     1081 aaatggagcg tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt
     1141 tttgttttag ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt
     1201 tgttattttt ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc
     1261 agaaaaataa aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt
     1321 ctcttaatac acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta
     1381 catttgttat cattattgca atgaagttta cctgatataa caaaattgtc caacaggata
     1441 cctttttcta atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa
     1501 gtgtttatta ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa
     1561 caggacgaga ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa
     1621 taaaatactt aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca
     1681 aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat
     1741 aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat
     1801 aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac
     1861 accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt
     1921 cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac
     1981 aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc
     2041 gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga
     2101 tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga
     2161 aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat
     2221 ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg
     2281 cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc
     2341 tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg
     2401 taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg
     2461 tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc
     2521 agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt taggatatga
     2581 ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg
     2641 gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg
     2701 tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat
     2761 gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga
     2821 ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca
     2881 taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca
     2941 aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg
     3001 aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt
     3061 ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat
     3121 tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca
     3181 tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat
     3241 cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat
     3301 gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc
     3361 ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg
     3421 tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca
     3481 aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca
     3541 gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa
     3601 ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag
     3661 atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat
     3721 tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat
     3781 tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc
     3841 aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg
     3901 aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac
     3961 agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac
     4021 tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga
     4081 aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac
     4141 aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc
     4201 aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg
     4261 aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac
     4321 acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg
     4381 cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt
     4441 cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa
     4501 agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga
     4561 tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg
     4621 aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt
     4681 ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa
     4741 aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga
     4801 tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg
     4861 ggggactttc gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc
     4921 gttctgtttg tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta
     4981 agaggaggaa gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta
     5041 gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa
     5101 gggaaagcac aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa
     5161 gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga
     5221 atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat
     5281 ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa
     5341 agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat
     5401 tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta
     5461 gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga
     5521 tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt
     5581 catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg
     5641 tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt
     5701 tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt
     5761 tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa
     5821 gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag
     5881 cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc
     5941 ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt
     6001 ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta
     6061 ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat
     6121 cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt
     6181 cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt
     6241 ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg
     6301 aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga
     6361 ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa
     6421 tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa
     6481 catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat
     6541 catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact
     6601 catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag
     6661 atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca
     6721 tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga
     6781 tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga
     6841 ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt
     6901 cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat
     6961 tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc
     7021 cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg
     7081 aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa
     7141 aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg
     7201 gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag
     7261 tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa
     7321 cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta
     7381 gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta
     7441 cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat
     7501 gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc
     7561 atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa
     7621 aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa
     7681 tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag
     7741 cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct
     7801 ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc
     7861 catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga
     7921 gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa
     7981 atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa
     8041 ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg
     8101 aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg
     8161 acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct
     8221 ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt
     8281 ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta
     8341 atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact
     8401 ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga
     8461 tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa
     8521 cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag
     8581 tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag
     8641 cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg
     8701 cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac
     8761 aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt
     8821 tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact
     8881 atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc
     8941 tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt
     9001 atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca
     9061 tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg
     9121 ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag
     9181 ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc
     9241 catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta
     9301 tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg
     9361 atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag
     9421 cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac
     9481 ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg
     9541 aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag
     9601 acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct
     9661 tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat
     9721 gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa
     9781 tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc
     9841 aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta
     9901 gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt
     9961 tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc
    10021 ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg
    10081 gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc
    10141 ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag
    10201 aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag
    10261 ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa
    10321 gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc
    10381 atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata
    10441 tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa
    10501 gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt
    10561 aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc
    10621 attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca
    10681 tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag
    10741 tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat
    10801 cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc
    10861 aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca
    10921 agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt
    10981 gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat
    11041 ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat
    11101 ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga
    11161 agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg
    11221 cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt
    11281 ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca
    11341 aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg
    11401 aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg
    11461 aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg
    11521 gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg
    11581 gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag
    11641 gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct
    11701 tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc
    11761 aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc
    11821 attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc
    11881 attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag
    11941 atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca
    12001 gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag
    12061 aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg
    12121 taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc
    12181 agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc
    12241 ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg
    12301 gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac
    12361 gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg
    12421 aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg
    12481 aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac
    12541 gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa
    12601 atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca
    12661 agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg
    12721 tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta
    12781 ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag
    12841 atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg
    12901 agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc
    12961 tttgatgttt cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg
    13021 aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat
    13081 tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca
    13141 aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga
    13201 aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga
    13261 ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg
    13321 gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat
    13381 catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa
    13441 tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc
    13501 gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa
    13561 tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt
    13621 taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat
    13681 gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa
    13741 gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag
    13801 ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa
    13861 caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag
    13921 gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata
    13981 ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg
    14041 agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg
    14101 gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga
    14161 aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata
    14221 ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat
    14281 aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca
    14341 gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt
    14401 tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt
    14461 tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc
    14521 gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt
    14581 aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa
    14641 gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt
    14701 tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa
    14761 aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg
    14821 aataccattt atttggaata
//
LOCUS       DAFEZB010000066        14650 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFEZB010000066
VERSION     DAFEZB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.066"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(459..821)
                     /locus_tag="LP859_002162"
     CDS             complement(459..821)
                     /locus_tag="LP859_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030091.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(895..1275)
                     /locus_tag="LP859_002163"
     CDS             complement(895..1275)
                     /locus_tag="LP859_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL3030092.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1339..2532
                     /locus_tag="LP859_002164"
     CDS             1339..2532
                     /locus_tag="LP859_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3030093.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2609..3193)
                     /locus_tag="LP859_002165"
     CDS             complement(2609..3193)
                     /locus_tag="LP859_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3030094.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3394..4392
                     /locus_tag="LP859_002166"
     CDS             3394..4392
                     /locus_tag="LP859_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3030095.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4504..5013
                     /locus_tag="LP859_002167"
     CDS             4504..5013
                     /locus_tag="LP859_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL3030096.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5043..5702)
                     /locus_tag="LP859_002168"
     CDS             complement(5043..5702)
                     /locus_tag="LP859_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL3030097.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5704..6468)
                     /locus_tag="LP859_002169"
     CDS             complement(5704..6468)
                     /locus_tag="LP859_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL3030098.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6476..7153)
                     /locus_tag="LP859_002170"
     CDS             complement(6476..7153)
                     /locus_tag="LP859_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL3030099.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7190..8575)
                     /locus_tag="LP859_002171"
     CDS             complement(7190..8575)
                     /locus_tag="LP859_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL3030100.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8785..8869)
                     /gene="ffs"
                     /locus_tag="LP859_002172"
     ncRNA           complement(8785..8869)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LP859_002172"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8892..9407)
                     /gene="tadA"
                     /locus_tag="LP859_002173"
     CDS             complement(8892..9407)
                     /gene="tadA"
                     /locus_tag="LP859_002173"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL3030101.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9514..10371
                     /locus_tag="LP859_002174"
     CDS             9514..10371
                     /locus_tag="LP859_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030102.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10452..10787)
                     /locus_tag="LP859_002175"
     CDS             complement(10452..10787)
                     /locus_tag="LP859_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030103.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10854..12224)
                     /locus_tag="LP859_002176"
     CDS             complement(10854..12224)
                     /locus_tag="LP859_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL3030104.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12302..12838)
                     /locus_tag="LP859_002177"
     CDS             complement(12302..12838)
                     /locus_tag="LP859_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3030105.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13147..14628
                     /locus_tag="LP859_002178"
     CDS             13147..14628
                     /locus_tag="LP859_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030106.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4504 a         2911 c         2376 g         4859 t
ORIGIN      
        1 tttttcgatt tcgtttttcg aaatctttca gcaactcatt tatcataact tatcgtttgt
       61 tatttgtcaa gaactttttt gaagtttttt tcgatagaat caagcttcgc aagtggttct
      121 tttttaacga ctcagttatc ttatcatgtc gttagtaaca tgtcaataac tattttttaa
      181 attttttaga aatgtgatat aacatccact tcagaatcaa cgctgttaaa tttatcatgt
      241 atttcttcct tggtcaacta taaattaagc attactttgt gaaatgattt ttcgatcaat
      301 tattattatt cgttatacaa ccgttttcaa catattttga aaataaaatg cactaaaaaa
      361 ctatctttag cagaacattt cattgtattt tttggaccaa acaataaaaa aagaagtttg
      421 aaaaaaattc aaacttcttt gttcgcttaa tctgtatttt agaagaaagg cactccctct
      481 acttcgtaag ttccaagaac agagcgcatt tgacctggtt cttcccaaat atatccttcg
      541 cctaattctt ctctttgatt cttttgttca gtttcaaaga caaacaacgg aacattttcc
      601 tctttaatat gtgtatttgt caattccatc aaactaatct tgctaacatc gatatgaact
      661 atttctttta agaaattaag tatattcgct aaaccagtct ttccttcaga aagttccgct
      721 aacgcaaatt ccaacttgtc atcaattgaa tggaccaaaa attttttagt accatcctcc
      781 aaatgcaaca taattgtacc agcaactctt ttgcttgcca aaatttccac ctcgatcaac
      841 ttgttctatg gcttacataa aaattctagc acattttcag aaaaaataca ctttttattt
      901 cttattttct ttccgttcta gttcagataa tgcttcttct atccaaatag gtaactgctc
      961 cgcaatagtg gtaaatccga tcttacgatt tttattcgtg aaataccttt ttctgcgata
     1021 tggaacagct ggatttgttt tttctaacgt tcttaaagat aaactgattt ttttggaata
     1081 ttcatcaatg tcgatgatct gaactgttac tggctgtccg acttttaaca atgaatggat
     1141 atttttcgta taaccggatt gtacttctga aacatgaata agtccttgcg tctcgttatc
     1201 aagagaaacg aatgcgccat aaggctgtat ccctgtcact tttccttcaa ttatttgtcc
     1261 aattttataa gtcatctact tccctctctt cgcatttatc attatactgt aaatagctca
     1321 aaaaaagaag cgacgaaaat gactggaggt cttaagatga tcgaatttga taaacagatt
     1381 tcaagaaaag atacagaaag tgtaaaatgg gacgcaattg ctgagacata tcaaatggat
     1441 gacttacttc ctttgtgggt agcagatatg gacttcttgt ctcccggtgg ggtagctaaa
     1501 gcgtttagtg aatacatcaa acatggaatc tttggttatg ccactctgtc tgataaacta
     1561 tatcaggcta tcattcattg ggaacgccaa caccatgctg tggagttgac gaaagaaaat
     1621 atcgttttta caagtggtgt gttaaccagt ttagctgcag cagtacaaac ttttacaaaa
     1681 ccaggcgatt ctattttgat tcatgaccca gtttatcctc cattttcctc tattatagaa
     1741 acaaatcaac gtcacatcgt tcgaagctct ttgctagaaa aaaataatca tttcatcatg
     1801 gacttgtctg acatggagaa aaaaatcgaa gaaaacaatg taaaagcaat gattttatgc
     1861 aatcctcaca atcctggagg gcgtgtttgg agcaaagaag aattgaaaca gttaagcgaa
     1921 ttgtgtttaa aacatcaagt gattctgttt agtgacgaga tccatcaaga tttaacttta
     1981 ttcgatcata catttacttc catgctaacg attgatccta atttagatga tctactagtt
     2041 gcttttactt ccgcaaccaa gactttcaat ctagctgcaa tcaaaaattc aatggtattt
     2101 atcaaaaatc cagagatgaa agctgatttt gaaaaacatt tagtgatgaa ccaacagcac
     2161 gaaatcaaca cttttggact gattggaacc caagcggctt atgagacggg tggagaatgg
     2221 ttaagccaac tgattcctta tctagaaaaa aatgttcaaa cagttaatca gttttttgaa
     2281 cagtatcttc cgaaagtgag aataatgaac cctgaaggaa cttacttaat gtggttagat
     2341 tttaccgcct atatttcaga cgaccaagaa ttggagaaca ctcttgttcg gaaaggaaaa
     2401 gtcgtattaa atcctggcat cacgtttggt ccttccggtc atgggcatat gcgcttgaat
     2461 attgcttgtc cagaagaaac tttactggaa ggacttaatc gaatcaagta tgctttagag
     2521 acaatcgatt aaatcattta ttcaaaataa aaggacgaga ccttactagg tttcgtcctt
     2581 tttcataatt actccgtacc ataccaaact attctgaaat ttcaattgtt tcaatcacta
     2641 catcatgtac aggtcggtct tgtggaccac gttgtacatt tgcgatttca tctactgtgt
     2701 ccatcccttc tagtacatgt ccaaagactg tatgtcggaa atctagccaa ggagttccgc
     2761 cacctttata cgcttcaatg atttctgatg ggaatccagc accttctagt tgtccaagca
     2821 tattagctgg cacatttgtg ttatttacaa taaagaattg gcttccgttt gtgtttggac
     2881 ctgaatttgc catagataaa gctccgcgaa ggttgaacag ttcacgactg aattcatctt
     2941 caaaactttc gccataaata ctttcgccac ccattcctgt tccagttgga tcgccgcctt
     3001 ggatcatgaa atcaggaata acgcggtgaa aaatcacgcc atcataataa ccttttttgg
     3061 aaagttcaat gaaattttga accgtcttcg gtgctagttc agggaataat tgaacagtaa
     3121 tgtcaccacg gtttgttttg ataacagctt ttggtccttt ttcacttttt aaatctagtt
     3181 gtgggaatgc catattgttt cctcctaata tttgcatact tcataataat aataacataa
     3241 tacctaacct ctcgtaaaaa tactagattt gttttctttt atttttcatc actagataca
     3301 ttctataact taccgtttcg atttcgaatt caaacatttt tgagaagcat tctcaattaa
     3361 attctgctat agtgtgaaaa gggaggaatt tttatggaaa tctatgacat tacaatcatc
     3421 ggtgcaggtc ctgtagggat gtttgccgct ttttacgcag gaatgcgcca agctaaaaca
     3481 aaaatcattg acagtctacc tcaacttggt ggtcaattag caactttgta tcccgaaaaa
     3541 tatatttacg atattcctgg gtatccagca ataaaagcaa gtgaattaat cgatcaatta
     3601 gaaaaacaat taactacttt taaccataca ttccatttaa aagaagaagt tctctcgctt
     3661 actcgtgaag acgaagttat cgagattact acaaataaag gcatccatta ttccaaagca
     3721 gtcattttag ctttaggaaa tggttcattc cagccaagaa aattgaattt agataatgct
     3781 gaatcatttg aaaaccacgg attggattat ttcgtgaatg atttgatgaa gtatgctgga
     3841 aaaaaagtcg ctattgctgg aggtggtgat tctgctatcg attggacgtt gatgttagag
     3901 cctattgcaa gcgaagttta cttgattcat cgtcgcccgg aatttcgtgc gcacgaacat
     3961 agtgtatcac gcttgaaatc ttcatctgtc aacttgctta ctccctatct catcgatgga
     4021 ttatcaggca ataatgggga actgacagat atccggttaa aaaaagtgaa aagtgacgag
     4081 acgattgatt tgatgattga ttctctgatc gtcaattatg gattctcttc taatttagaa
     4141 catctttctt cttggggact tgatagtacc agaaatgcca ttactgtaaa atcagatatg
     4201 tctacttcta ttccaggcgt ttatgcagct ggtgacatct gtacctatga aggaaaagtg
     4261 aaactgattg ctactgggct cggtgaagca cctactgcag tgaacaacgc tttacactat
     4321 attaatccaa aggaacgaac tcagcctggt cacagcacaa gcttatacga taaaaatctt
     4381 cgtcctagtt gattttttat atctctttgc atttcaagtt aaacccgaaa gcagtttctt
     4441 gcattttttt catgttccgt taacatgaaa tagagaaaga aaaaataaag ggggagcaat
     4501 aatatggtcg tcaaaactga gactttagaa gaaaaggcaa gagaactatt gaaggaacgt
     4561 ggcgtaactg tagaagatat cgcacaactt gtcatgtttt tacaaaaaga ctatatttct
     4621 gatttgactt tggcggattg tactgaaagt gtcaacagcg tgctaacaaa gcgtgaagta
     4681 cataacgcca ttatcactgg aatccaatta gatatattag cagaaaagaa aaaattgatg
     4741 agtccgttgc aacatattat cgaagatgac gaaggccttt acggtatcga tgagatccta
     4801 gccttgtcaa tcgtgaatgt ttacggtacg atcggcttta cgaattatgg ttatatcgac
     4861 aaggtgaaac ctggtatttt aaaagaacta aacagccacg atggcgttca tgtccatacc
     4921 ttcttagatg atatcgtcgg agcgatcgcc gctgctgctg ccagtcgatt agcacacaaa
     4981 gaaccagaaa aaagaagtcg ccttactcaa taattcaaca aaaaaagaaa gaaaacgtct
     5041 atttagacga gttctttctt ttttgtttgt ttcaagctat tttttcctaa gaaaagaaaa
     5101 acagcaaaaa ataattctaa taatacaaaa aataagataa agctatgcat gaaaaattct
     5161 tctggaagta cattgatgat tggatcagta acgggatcga ataaccagtc atcgttgttg
     5221 aaaaacgttt catgaaaaag gataaaaaag cgatcaaatc ctatcatcat gaaaaaaccg
     5281 aaaacaactg gcagtaacat gccgatttga aacggacgaa tcaaccgcca aagccgatca
     5341 ttcttttgca aatatttgat aaacaaaata ctcgggatca aagtgatgaa aaacacagca
     5401 taatccacta aaaacaaaat tttcacttca taaaagtgat gggctccgct agcagaaact
     5461 gggaaatctg gcaagctgag gatggttttg aagggattat tgagataatt cattaggtga
     5521 tcaaaatttt tcagtaacgt ctcttgatct aaagaagtat aatccaaaat ttgcaaatgt
     5581 ccgatatcaa atacgtatag tggtcgaaag ttgatcgtca caccaactgc cagactgatc
     5641 agtgtcagaa ataggctgaa caatccagca tattccatcc actgccatct cgaatttttc
     5701 atattagaag ctccattcat ccagtgattg tttcacatag gtagctggga taggcaaccc
     5761 aggtacatct tcttcttttg taaaacctga taaaacgagc aaagtatcta tattattgcg
     5821 tatcccagct tggatatctg tttcatagtt atctccaacc atgattactt cttctttttg
     5881 cagccctaat acttcaacgg ctttatccat aatgatggct tctggttttc cgatgtagat
     5941 cggcggcgtt tgggttgctg tctctacaaa agagatgact gaccctgctc caggaagaag
     6001 gccgcgttca gtaggtatat tcttatctgg gttcgttcca atgaaagtag cacctttttg
     6061 aatagcaagt gttgctttaa ctactttttc ataggtcaga taattatcta agcctaccac
     6121 tacatagtca ggagcctctt cttcccatgt aaatccggca gctaaaatca agtcaatcaa
     6181 tcctgcttct ccgataacat agactttttt cccttttcca tctgctttca taaaatcgat
     6241 tgttgccaaa gtagctgtgt agactgtctc tggagctaca tgaatgtcaa actcatctgc
     6301 tagacgatta gctactgttt ctggtgactt agtcgtgtta ttggtaacaa acaagaaagg
     6361 taattttctt ttttgcaatt cttccacaaa tcgtttaccg gccggtatgg gttgtgtccc
     6421 acgataaatc gttccgtcta aatcaattaa atatccttta tagtccattc atccactact
     6481 cttccctttt tctgatcgta aatgctgttt ttgttttttc tacttgttta ggttgttctt
     6541 gtttttttac tttttcagag gtttttgctg actctgtttt tcgattttta ataaccggtt
     6601 ttttgcgatt agatgcgact ggtccttttt tctcatcgat atacgcacga ttatggtttt
     6661 ttttcttttt tcggcgctga gttgtctttt cttttttacc gccgattctc tcgatgacaa
     6721 aatacgcaca accaaaattg caatattcgt aaagatagtc ttccaaggaa tcgattcgct
     6781 gatctggatg cgcccgtcga ttatccgctt ggaaaaatcc ttttaggcgt agttgttcat
     6841 aaccccaatc tcctacgata taatcatatc gggctagtac atcactgtaa cgttctccaa
     6901 gcttttctgc attaaatcct tcacgataat cggacacaag ccgataatgt ctttcaccta
     6961 tcaaaaaatt actgtcatct aacagtgtca ctagctcgcc tttgactttt tctgctttag
     7021 gctcctcaac gatttcttcc aaagcagctg tcagttcttc ggtgactgtt gtttcctgtt
     7081 cagctgatga ttttttctta gcaatttctg ttttatgctc aacgttctgc tgttcatttt
     7141 gttttgcaga catgcgttca ccacctatct tttttattat accttgtttt tatttgattg
     7201 ggtagtgagc tttcaagtaa tctcctacca cacttcggat aaattcaggg aacaaaaatt
     7261 cattttttcc agcaatctca atcgttggaa aaaatggaac aaacatcaaa tgatctacgg
     7321 tagtaaaagt ataattttta ttcagatcga ttggcttgtt cttccaaaag acttgatggt
     7381 taatagaatc atatgtgatt ccattataga ccatctgccc aaaaatctta ccgcgaaatc
     7441 ccattccctt cataggatag ttacgtaaaa agttacgatt tttttctatt tctaaaacta
     7501 agcggatcag atcatttcca cccaatgtga catttaacaa gtgcatcgga tgcggcaacg
     7561 cagtgtgaag ctgatcctga ttgacccgcc cttttggaat ttcagttaaa aacagaccac
     7621 tgtttaatat tgctgcttcc gtctttcctc gttctttcac cgcctcaaag gctacttgga
     7681 taaacgaatg ctcattgaag atatctagac tcagagaaaa tggcaaatct gctacttctt
     7741 tttcttttaa taattcatgc ccacgttcta gataactatt tatttcttgt tcatcttctg
     7801 taaaagctgt cattgtttcg gtagggatgg ctttggctga atgcttaagg atttttttct
     7861 cttcatcgat ggttaaatgg acttcaccga catactgccc gtactttcca gcggctgcta
     7921 tttggacatc attgattacg tgaccttctc tgaataaatg atgggtgtgc gatcctaaaa
     7981 taacatcaat tgctggtatt tcttttgcaa tttggaaatc atcctgaatc ccgagatgac
     8041 tcatcaaaac taaaacatcc acttgtgaac gtaaatcttc gactaattct ggcaaaatat
     8101 cataaggatt ccgtatgtcc cagccattcg gattataagt aagcggaaat ggagcagtaa
     8161 atgcaattag tccgattttc gtctggtgtt tcgttgtaat gattttatat ggtttcgtcc
     8221 atgccggtgg ctggagcgta tttttatcga ataagttatc caggatgata tcaaactcag
     8281 catgatcgta taaatgattc aactcttctt tggaatttcc tacgccttca ttgtttccta
     8341 tcgtcgctgc atcataacca acttcattca tcagcaagat attttcctgt ccattcgttg
     8401 cttctgacaa tggatgccaa cgatccacaa aatcacctag atctactgca atcgttgtcg
     8461 ttttttcttt cttttcgttt tctcgtttac gttgatccaa aaaccttctg atccttggcc
     8521 aattttctaa atgagaatgc agatcattcg tatgtaaaat cactattttt tccatgtacc
     8581 ctctcgcctc tctttatctt tatcatacca taggcagctt tttcttccat tgaaacaagc
     8641 tctatttcag agtttttcct gccttgaagc aaattgtata agtacaatat tcgataaatc
     8701 agtctggcta aaaaatcagg aacattcatt tttacagtac tataaaaata aaaaaaacag
     8761 acccgaaagt ctgttgtcgt ttcattataa tcagacacat ggcggaacct gcttagtgct
     8821 gcttccttcc agacctgaca cgcttcacaa gcccaccatt gtcctagcct ttcggcaaag
     8881 aatagtatac cttatttttc attcgttgac cagtcttttt ttaatttctt ttttcgctgg
     8941 cggagctttc ggaaaaagtc agtcaaaatc atcccacatt cgtcttctaa aatgccacct
     9001 tctacatatg cttggtggtt aaaccgctcg tcctccaaaa gattcatcaa ggtaccggct
     9061 gttccgcctt ttggatcata cgcgccgaaa taaacttctg ctacacgaga caaaatcatc
     9121 gcgccgctgc acataggaca aggctctaag gtaacaaaaa gtgcagcatc ttccaagcgc
     9181 cagcttccca ctttttcaca ggcttccttg attgcaagca tttctgcatg tgtagtagca
     9241 tcttgcgtgg tttctctaag gttatatcct cttccaatca cttttccatc caatacaacc
     9301 actgctccaa tcggaacttc atgaagcatt tctgctttct ttgcttctgc caatgcaagg
     9361 cgcatccatt tttctttttc tactgcatca agatttgtat ttaccaatta cccttccctg
     9421 cctttgtttt ttcttgtttt actttagcat atgataaaaa ggctgtaact aacttttttt
     9481 tctatggtaa aataaataca agaggtgaac aagatgaaaa tggataaatt acttgctctt
     9541 tacccacaag cacagaaaaa aaactctcca gcatcagaag cggatatcct ttcttttgca
     9601 attgatgatg cttttatctg gatcaaacaa gattcattat ctcaacaaga aaccagcctt
     9661 ttgaaagcgt tattccctgt aatcaacgat caaaaaaaac acccatggta tcgctatctt
     9721 tttaaagatg cttcttgtgt tcaagaaaaa agctttcgtt tgatccagct tcacgtcgaa
     9781 tcaaaaggag aattcttaaa aaaagaatgg caagatacta ttttggaatt atttcgctat
     9841 tctgctgatt tcttttttta tacagacaca gatgcgctct tagtcgaaca aataaaccgt
     9901 gattatcttg atgctactga aatcaacggt atttttctat ctttagatgc tgattttgat
     9961 acgacaacgt ctgtattcgt tggttctttc cattcacctg gcaccgacct cgtccctttg
    10021 tttgctgaag aaagaaaaat ttttttaaca gagcaaaaca atctttatac tcacagccgt
    10081 actttttcta tgcaagatgt ggctttacat tactatacaa aagatactgt aaaagatagt
    10141 aagttgatca aaagttacat agaactgatc aaacaaaatg aaatggaagt gatcattcaa
    10201 gaattatgga atgaattagg caatgtcagc tctgctgcaa aaacactgtt tcttcatcgt
    10261 aatactctaa aatatcgaat cgaaaaattt caagaacagt ctggtttcaa tctaaaaaaa
    10321 gccaacgatt tactgttttg ctatctttta ttgcttcatg aacataatta aagatagatt
    10381 aaaggggttg tgacaataat tttgtcacaa ccccttattt cgaatgaata gtgttcaaaa
    10441 gctagtttct cttacatcat tttttcaaca aaatgcatta gccggtcttt tacatcttcg
    10501 gttgaatctt tgattgaatc accataaaca gagattttct tttttcctga tttgattttt
    10561 gctaacattc ccatcaaaga atccaagtca ctatctgaaa gaccatctac gatttctagt
    10621 aatttttcat ttccatcaaa tttgtcatag acgaattttt ttactttcgt ccgagtagtc
    10681 atatgatgga tcttttccat tacttttccc gatacaacga ctgctgtcgc tacactggca
    10741 gcggctgcta cgcttaatcc gattgttact tttgttgatg ttttcataag tatcagctcc
    10801 tttttatcta tctccatcat agcatagaca aaaaaataaa cgaactaatc cactcagtga
    10861 aatctcaatc ctttagggaa aaagttcttt actgttcctt ttacttgttt cccaaatccg
    10921 acaggtatct tatctaatac tagcaaaacc catccttgat tcccatcacg ttgaaatgtt
    10981 tctcctgctg tgtaagattg ccattccttt tgagtgatcg gcaagcaagg tatattctct
    11041 atcttttttg tagcaagcgc taatgcatag ctaggttcaa atcgattctt tttgaaatct
    11101 cctaaatgca aacctgtacg tacgactttt aaaccatcga gagaaggggc taactcagga
    11161 acttcccaaa gatggtcgtt gaacactaaa agtcttccag tagcttcata atgaaaatca
    11221 tttgaaaact ccgtccagag tttttcctgc tcttttgtca tttgcacttt actttttttt
    11281 cgtgtcttct tttctttatg catctgattt tgtccatgaa aagtcagctt tgcaacaaaa
    11341 tgtccttcac cctgatcttt atgaggccat atacgtattg ttttttccaa accagctacg
    11401 cttccccatt cagaacgtcc tgaactaacg gattgagtca aaggaatctc ctcaatcgtt
    11461 actggataat tttctacaag ccagctaatg atctcttcat tttcttctgg tgcaaacgta
    11521 caagtcgaat aaatcagttg tcctttattt tttagcattt ttattgctga agacaaaatc
    11581 tcttgctgac gtttttgaca gtatagcgga gattcttctg tccactcttt tattgcattt
    11641 ggatcttttc ggaacattcc ttctcctgaa caaggagcat caaccacgat ccgatcaaaa
    11701 aagcccgaaa aatgagggac aagttctgcg ggtgcatgat ttgtcactat tgcgttcgaa
    11761 actccccatc gttctatatt ttctgataaa atcttcgcac gtttcggaaa gatctcattc
    11821 gtcactaata accctttccc cttcatttga gcagctaatt gggttgattt accaccggga
    11881 gccgcacaca aatccaaaac tttttcaccg ggttttgctg cagcagctgt tccaacgatc
    11941 attgcacttg gttcttggct atattcatac ccagcttgat ggaggaaaga ctttccattg
    12001 acagtgccaa gaaatccctc attgctgtag ggagcgggtt gtaattcttc gctatggtaa
    12061 gtttggacca tgtccaatcc tgccggtttt agtggattcc accgaaatcc ttttttcacg
    12121 cttccttgtt caagcgcaga aaaaaaatcg gatgcttctt cgcctaaaag aagacgatat
    12181 tttttgataa attgctgtgg taacgttgtc gcttcctcct tcattccaat gtttcgttag
    12241 acattttaca atctttttta cgaaaaataa aggtggctga tctacttttt catgttgctg
    12301 cctaaggtga ctgttccttt aagatatttt ccaagtattc atccgtcacc tcgtttttat
    12361 cccaaacagc gttcatcatt tcaagaaaat cttgagaaga atcatctgag cgcaaacttc
    12421 ccaatgcatt aagccatacc aattcaagcg acttttcttc tttgatcttc gtgtgacctt
    12481 tgacatatga gaaaaatggc cgcttaaata gcgaatccat gtctaaatca gattttgcaa
    12541 tgatttcacg aatactttca tggatagaaa aaaaatcttt caataagtag acaatgctta
    12601 aaacatcatc ggaactgcat tcagctaagt agttttcaaa ataaacagtg atttcttcta
    12661 atatcttctc ttttaacccg ctgattgatt tgaaatgtgt ataaatcggg tatgtagaac
    12721 aaccgcagaa ctctgctaac tctcttgcag taattgactt cattccatat tcttccacgt
    12781 ataccatact tttgaacagc aaatattctt tactataatt tgttactctc cccatcattc
    12841 ttacctccgt ctcttttcat ctacccataa ataaataaca taaataattc taaaaatatt
    12901 attgataatt atcaaaacat atttagttat atccattaaa aatcaatttt tccgccttaa
    12961 aaacaagaat atcttgacaa aataccctag gaaataaaca atagaataaa caaggtaaca
    13021 aaaaaattaa ataacgttat ataaaaaaga aggcgacact ctgtgagaat aacacagact
    13081 gaaatgactt tccactcaca gtttttgtct aactatatac aaaaacagaa taaaaaggag
    13141 gtatttatgc taattgaagc aagaaaaaaa gagttacttg ttctacaaat ggtcattcaa
    13201 aaaaataatt gttctttaca agaattatcc aatgacttac atattccaaa acggacaatc
    13261 aaagaactga tccgaaaaat aaatgtatca ctcaagcaat ttttattgat tgatgaattt
    13321 atttattcta atcataaagg agagatccaa accaaagaaa ccgatcaaac taaaaagtta
    13381 gctgtttttt ctaagttaaa attatgctac ctaaaagaat ccaatcgttt taactatttg
    13441 atgttactaa tcaattatcc acaaactgct gtaccaaaaa aatatttact tgaacaactt
    13501 tatatatcgc cctcctatct cgaaaaactg acaaaacagc tcaatcattc cttaaaaaaa
    13561 ttccatttac ggatcctatc tgtaaaaaat tgctgtactc ttgaaggaaa tgagttatct
    13621 atccgtctct atctttactt ttttctttca gatacatttg gaggtaacga ttggccattt
    13681 tcctcacttg aattaaatgc actaaaaatg aacagtacag atgagcaaat agaaaaaatt
    13741 tcaaaacaag aaaaaaatat ctacttgctg ttaactttat tcatattacg aaacaaacaa
    13801 caggcatatt ttccaaaaac ttcttctgaa atcactgaat tgatggactt attgcagcat
    13861 acacatgatt attcagaata ctttacttct ttttcagtga gtcgcccttg tgaagagaca
    13921 gaaacagaga gagcctactt taattatttt ttctattgtt tttttccaaa tcaaatctct
    13981 tttcgacaaa aacaggagat tggtttttta ttttccacaa gcaaaaacct tatttgtcga
    14041 caagtcggtc aattgatgac ttacttaact caacagtttc ctctcaacca tttagaaaat
    14101 aaaataccaa tttattttta ttttcttgtg ttggcagttg tttttttcga actgttgggt
    14161 gaatccagca gtagtttttc tacactttgc tttccagaag ccacgtccaa tcattctagt
    14221 tcttttatac aggaaaagaa attaagagaa aaaatcgata caattttttc aaaaaatggg
    14281 gaaagaaacc aaatcagtca ataccttagt caattgatcc aacacttact acaaacagaa
    14341 acgaaagagc agttactcat ccatttgcag ataaccaaac agtttacagg aaattatcat
    14401 cttcaaagcc atttgcagca gttatttcgc aaagatgctg ttaaaattac cgcatattca
    14461 gaacatgctg atttgatcat tacagatcag catgagaaaa tttcagatga aacggagact
    14521 ttctatctgg atacaacgca caatacagtg ttatttggca agttaataaa agcggttcat
    14581 gaactgtata tgaaaaaagg ttatgagaac aaaacaaaga ggatgtgaca aaattcttgt
    14641 cacatcctct
//
LOCUS       DAFEZB010000067        14624 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFEZB010000067
VERSION     DAFEZB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.067"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            3..815
                     /locus_tag="LP859_002179"
     CDS             3..815
                     /locus_tag="LP859_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL3030107.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="LP859_002180"
     CDS             812..1612
                     /locus_tag="LP859_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL3030108.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="LP859_002181"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="LP859_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL3030109.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="LP859_002182"
     CDS             3109..4818
                     /locus_tag="LP859_002182"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL3030110.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="LP859_002183"
     CDS             5030..9388
                     /locus_tag="LP859_002183"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL3030111.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="LP859_002184"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="LP859_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL3030112.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="LP859_002185"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="LP859_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL3030113.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="LP859_002186"
     CDS             11226..11519
                     /locus_tag="LP859_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL3030114.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="LP859_002187"
     CDS             11516..11827
                     /locus_tag="LP859_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL3030115.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="LP859_002188"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="LP859_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL3030116.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="LP859_002189"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="LP859_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL3030117.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3255 g         3789 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatca ggctctttgt caataaggac tgatgagttg
    14581 tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DAFEZB010000068        14225 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFEZB010000068
VERSION     DAFEZB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.068"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..278
                     /locus_tag="LP859_002190"
     CDS             <1..278
                     /locus_tag="LP859_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030118.1"
                     /translation="FLNNNLLDGREIQLIFTAKTPLQSEKNIDNQAVVAVDNVSNKTN
                     VVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLLLVLATFGYAVLKFN"
     gene            complement(314..2371)
                     /locus_tag="LP859_002191"
     CDS             complement(314..2371)
                     /locus_tag="LP859_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL3030119.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(2516..2707)
                     /locus_tag="LP859_002192"
     CDS             complement(2516..2707)
                     /locus_tag="LP859_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030120.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            2895..3350
                     /locus_tag="LP859_002193"
     CDS             2895..3350
                     /locus_tag="LP859_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030121.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            3347..3589
                     /locus_tag="LP859_002194"
     CDS             3347..3589
                     /locus_tag="LP859_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL3030122.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            3607..3909
                     /locus_tag="LP859_002195"
     CDS             3607..3909
                     /locus_tag="LP859_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030123.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            3978..6032
                     /locus_tag="LP859_002196"
     CDS             3978..6032
                     /locus_tag="LP859_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030124.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            6032..8644
                     /locus_tag="LP859_002197"
     CDS             6032..8644
                     /locus_tag="LP859_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL3030125.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKKAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            8641..11019
                     /locus_tag="LP859_002198"
     CDS             8641..11019
                     /locus_tag="LP859_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030126.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            11080..11622
                     /locus_tag="LP859_002199"
     CDS             11080..11622
                     /locus_tag="LP859_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030127.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            11700..12032
                     /locus_tag="LP859_002200"
     CDS             11700..12032
                     /locus_tag="LP859_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030128.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            12033..12626
                     /locus_tag="LP859_002201"
     CDS             12033..12626
                     /locus_tag="LP859_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030129.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12648..>14225
                     /locus_tag="LP859_002202"
     CDS             12648..>14225
                     /locus_tag="LP859_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL3030130.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRL"
BASE COUNT         5003 a         2264 c         2880 g         4078 t
ORIGIN      
        1 acttcttaaa taacaatctt ttagatggac gagaaattca gctgatattt acagctaaaa
       61 ctccattaca atcagaaaaa aatattgata accaagccgt agtagcagta gataatgttt
      121 ctaataaaac aaatgttgtg acaattgggg ttgatcctaa cttgccacaa gttattgttc
      181 ctaaaacagg ttctacacat ttagtaacga ttttagcggt cagcttacta ttacttgtgt
      241 tagcaacttt tggctacgca gttctgaagt tcaattaaac aaaaaaacaa ggctgaaaat
      301 cagccttgtt tttctatttg aaaatgggat tggcactaat aaatacgttg tctgaagaaa
      361 gtgatccgtc tgcagaaact tcataagcat gggataaacc aacgatactt tgattgttgt
      421 aaagcaagtt tgcgttatga gcagcgctat ctttccacat aaccatagca tttactgctc
      481 ctgtatctga aaggatctcg tcaacgtaaa ggtagaaact attgatattt tctcctttag
      541 caccgtagca gcctaagctt tctaagagat cttggaaagt accgccgcca ggtcggtaat
      601 gactgaagct tacttgtaat tcttttgcac gaatatctgc agcttgttga acgatatcat
      661 tgaattgcat tggggcaacg ccattttgtt gacggtagct atttaaccaa gcaagttctt
      721 tttgacgcac atagttgttt aaatcactta gtgagatatt tttgtatttg aaagtgatcg
      781 ttcgattttg gtcaacatta ttgattacac gttgtccatt tttatcaacg gtaacagatg
      841 aatcgtcatt tctacacaag aagtagtttt gatcggtcat atttttccaa gaagtagaga
      901 aactatctcc ctcgtaaacc gtttgtgttt cagtagataa aacagcgttt gtgtcaacgt
      961 ttatatggtt cacagtgacg ttgtattgat gagtataggc aaaattgatt ccttgattac
     1021 ttgtaacgtt gtttaaagtc acagtgttgc tagagctttc tctaggatca agaacaaaat
     1081 tagtgaacat aggtgcttca attgtgatgc tttgtccata aacgacggga taactcttac
     1141 tgtaattata gagttcttgg atatttggat cagtatccga gactttattt ggacgagctt
     1201 tcacttcaac tagaaagact tcatcatagt aaaaagtaaa tgtggtattt gtgttaatcg
     1261 tttgtgtttg tgtttcattc ccatttactt gatactgtgg tgcttcaaag ttagtcaagt
     1321 cggttctagc ttttgcagtg taacttgtac cagcttttac tgagacatgt tcttctttta
     1381 gcagcgttcc agtttgtcga ttcatatgac ggacagttac cgcatattct gaaggtttag
     1441 gtttgtgttg taacccttta aatgcagaat caagactatt tttagcatta tttacttggg
     1501 tttgagaagc tttagaatcg tctagtactt ttttagcatt ggataaagca gtctgaaaat
     1561 tattccaaga tccttctgta aaatctcctt tagcagttga ttttacttgg ttgtacaagg
     1621 cttgtaattc cgttttgttc acagtaggag ctggtttttt ctttaatccc ttgtaagcag
     1681 aatctaatgt gtttttggca ctatccactt gtttttgagt cgcttttgag tcatttaaca
     1741 cttttttagc attgtttaaa gcggtttgga atgcgttcca ggtattgtcg gtatagtcac
     1801 cttttttagt tcccttaacg ctcgtataaa gagcttctaa agctgattta ttcactttgg
     1861 gtttagaggt gtcagcacca taccaaccaa taccttcgta tttccagcca tagtctttgg
     1921 ttaaccattg tacttctttc caacttgcag tgtagtgatg cgaaccagag gtaacacctg
     1981 gaaggaaaag gcggtagatc ggtttattat ttttttcggc agaacgccag gcaggccctt
     2041 cataagtcca accgtaattt ttggtcaacc atttcacttc atcccaacta gctgtgtaat
     2101 ggtgatcacg aaggccttta ttgtataaac gatagacaag gtggccaccg ctagtcggtg
     2161 cttcccaagc ggggccttca taattacccc acttaatttt cactaggtga tcacgttctt
     2221 ttgtgctaga ggtgtaaaaa tgttcatggt tatttggatt ataaaggcga tacatgacgt
     2281 tagttgaagc agcgtcagca gatccagcaa gagcaactgt ggcagcaaat gttccaatag
     2341 ctataacagt ttttgggttt aataatttca tgttttcaac tccctattaa atgattaaat
     2401 attttgttta cattcataat ataccacggt ataccgaggt agtcaacggt ataccgtgga
     2461 aattttgaaa taaaaaagcc gccctatgaa gagtgtaagg atggctgttt atatgctatg
     2521 aagaaatgga ttcggcgtat ttggttagtt tggcagcatt atcaaatcgc attttttcaa
     2581 tcgcactttt tttctttatt aaatccgcaa ttgttgattc tgcgactgaa gaatgtttgc
     2641 taatttcata tgctgtcttg ccagtttcaa atagtaacca ttcgattttc tctttatcag
     2701 cgtacattgc aatgctccct cttgattttt tctttattat agctcttttt tgtaataaat
     2761 cacaatatca agaggaaaag tttctataga aaggtgggaa attatgagta tccgttcccc
     2821 gtgtatcaaa atgaggtttg ttgctacttt cgttatttaa tatactaaaa taaaacaacg
     2881 aaagaaggaa tagaatgaaa aagttaataa tggtatttac ttcgttgatt gccatgattg
     2941 ttattttagg tggatgtggt agtgaaaaaa ataagcaaga tagtcaagat aatggcgtgt
     3001 taaaaccagg aacagtgtgg aaagaagaag taggcggact tgttcataat ttaaaaatta
     3061 tagatgatac aacatgggaa tattccgaaa gtgtttggca tcctgaacaa gtacaaataa
     3121 cagttgagaa gctaaaagac tataaaggat tagaaagata taaggtaatc gattcaggtg
     3181 gagtagattt attcataggt aaaacagcct taatagttcc ctataacaaa gacggtttag
     3241 aaacgtttaa atttattcca acaaacgaaa aagaacatcg agataaaagt gaaatattaa
     3301 aagaatacag tactaaatct ggatacaagt tacaaaagac tagcgagtga ttgctagtct
     3361 ttttatttat cctgaaggag gaatcgcagt ggcaaaagct aaaacgccat ttgaaaaagc
     3421 cgcgtctaaa atcttagaac gtcgcggtgt atctgaaaca cagtggaaac aagaagtcct
     3481 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     3541 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     3601 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     3661 gttgatattt ttagagcatt acaaggtgca gcaactagtt taaaaaacaa tctgatgatt
     3721 tttgcggctg tagctgccgt tgcctccatt gttcttgggg ggattttctt cttctttggc
     3781 cgaagaggag ctgaaaacgg gaagatgcac gtaggttatg tattgatggg ggtaggagtt
     3841 atattagctg ccgcctctat tgtggtttcg ttatttggaa tgtttggaca aaccccacag
     3901 ggactataaa aaataaatag aaggagtaaa aagtacgtgg ctacttttta ctcctttttt
     3961 atgaaaggag aatactcatg tactttacga tttcaggcaa taccaaaaaa gataaagagt
     4021 taataaaaaa agcaacgaag aaagtgggaa agcgtactgc ctttttaccg aaaacaatta
     4081 aatattctgt cattgaaacg gaagaacagc tacatgtgtt aggacaagtg ttagaacaag
     4141 agaatcaatc ttctgttaaa ttgactctca cgttacatga taaagaagga aaaatagaaa
     4201 aggctggcac agaatacgtt tttggcccac tggattatca aatttcagaa ggtttttatc
     4261 ctacattgat ggatttgatc cgccttgatg tgtttaagga aacggatgaa accatgagtg
     4321 tggaagagtc ttatcgaaac gaaaaactgc tacaagtttt agaagaagag ttagagagcg
     4381 accaaagaaa agtgagtcaa gtgagtgaag tcgaagtaaa tgatgaagta ccatgggaac
     4441 gaccaatctt tccaaagaag gagtcaaaag aaaaagagac agtctatgtt gacgaacaag
     4501 ctgatattcc gactcagaaa tcgatatttg aggaagagaa cgatgtaatg ggtcaattgg
     4561 aggtagatcc ctttgaagaa acaatggagg atgaagagac ggccgaagta gaagttgatc
     4621 tagttgaagc taatgaagag ccaacatgga ctgaagggga ttctcaggaa tatattgaac
     4681 cacttatcag tgaagaagta gagaaaaacg aaacagagat acaaaaggca actgtatttg
     4741 attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa aataaattat
     4801 ttttaaatga gctttattcg aaatatcaat taccccaaac ctatcaagca tttttagaga
     4861 gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta gagtctgttc
     4921 aatcaacaaa ttggcatgag cttgcacaag aagatttatt agccactttt gaagaacgtc
     4981 aacaagaaac agatcaagaa ataaatgagt atgcgacgga acagtcatct aattgggaga
     5041 gaacaaaaga ggaactaaat aaagaagaac aacgagtgat tgaagaagaa gtacaaaaaa
     5101 ttaaagagcg atacgaaaag aaacgcgaca atgcgtattc cttgtgtata gagaaaatta
     5161 ataactttac taaaactaat caagaagctt tatcgaaaga aaaagaaaac ttgttaaaag
     5221 aacgtataaa agaacgaaaa gaagagttct accgaggttt acgtacagat aagctaaaga
     5281 ttagcaaaga attaaatgaa caactgaata aactttatca aacaacgact gaagttctta
     5341 tggaaagaca cgggcagatc cagcaagaat tacaagcaca aattccaaaa tggaaaaaag
     5401 agtatgaaat tcaacaacaa gaattgaaag aacaagaaga aaaacaacga caagaagaaa
     5461 aagagcgcga acaattagaa ctaaaaaaac gtgagcagca tcttgcagaa caaaaagtgg
     5521 cgatagaaaa acaacggtta gagcaagaaa aggaaaaaga agcagctgct cgtaaggaac
     5581 gtcaagaaca aatggataca ttgcgacaag cacagttgaa ttatctagca caacctcaac
     5641 cagtagctgc tgttcaaacg ccagtgaaca accaagcaaa tttaactcct tcaccacagc
     5701 gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag
     5761 gaacagtatt tgcttttaat catatcaatc aatcttcaca ggcagattct gtagccacaa
     5821 cggcgtcaat caaggaagaa gcccaggaac aagcaagaaa agagtatgaa caacaattgg
     5881 aagaattatt gaatcgtacc aatcagtcaa cagcagagtc aagtatggaa aatagcaaag
     5941 agagtgatcc tagtaaggct aaagaaggat caagcgattc aactgtacac tcaaacactt
     6001 ccgaaagttc agaatcaaat gaggtgaaat aatgcgtatt agagaaacaa cgagacgaaa
     6061 agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc gctatactcc
     6121 ttcaggagaa gagctaccag aaaaacatga caaacgtatt tctcaaaaga ttttttatag
     6181 tggcttagct tatcttcctt taaactatgt tgttacaaga ataccagaag catttcatcg
     6241 atttgagatt attccaggaa gtttacaagg agtgaatatt tttcagttac aagaaatgcc
     6301 gcagttattc cattttgatt caactggttt ttggaatcct ttgaccccaa caacaagttt
     6361 aggcacacgt ttagttgttt ctacgatttg tgttgttcca gctgtactta tgggattgaa
     6421 aatggacatg tggtttcgtc cgctagcaga tgggcaaaaa ggagataacc gattaaaaac
     6481 gttaaaggaa atccaacaaa tttatccgca aataccagaa tcaaagggaa gttttcctgg
     6541 ttatggcgga ataccaatta ctcattataa aaaatactgg tatattcaga cggatacagt
     6601 aaatacttgt attgttggta cttcccgctc agggaaaggc cagatagaag ttttagcaac
     6661 cattgacaat ttgtcgcgtg cagaaagaca aagttcaatg gttgttaatg atccaaaaac
     6721 agaattatat gttgctagta aagatgaact agaagctaga gggtatgatg tgtatgcctt
     6781 taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca ttgtgaaata
     6841 ctggaagcga ggagacatag acaccgcaac acagttaacc aatactttta cgaatacgat
     6901 ttattatgat gcaaatgctt cagacaataa atactttaac gaaaatgcgc agaaagctgt
     6961 caatgcgctt atttttgcgt tattggaaca agcggatcaa acaggggatt actccaaatt
     7021 aacaccaaat aatctcgttg aactactttc agaaattggc ggttttaatt attatccaga
     7081 tcctgagaat gaattcaagc agctcaatgc cttagatgag ttcatgaatc agttaccccc
     7141 tggaaatata gcaaaaaaac aatatggggc aacaaaaatt ggatctgata aagcgaaagg
     7201 gaatatcttg tctacggcaa ttacaggatt gaatccattt accctaacga aaattgcgaa
     7261 gatgacttct caatcgacca ttgattacaa gtccattggt tttccgaagt atttggatct
     7321 caaattacat gaaagcctat taaatcaacg cattgtcgtg gaattttatc ataagggaaa
     7381 gaaatttcac gaggaacgag tcaaagttgg ctatcgtggt ttttgtgaaa tcaactttga
     7441 ttgtcattta gagcagggag atcatgtttg cttaaaatac aatcatcaag gaaaacaata
     7501 tgttgcttgg tatgagtttt ctcaaaaagt attgaaagac gaagagggaa aaatcgttta
     7561 tcgaaaaaaa cgaggagaaa ctcatttgcc agaactagaa aaaaaagccg ttctaacatt
     7621 agatgagaag aaaagtaacc tattagtaga gtctattcgg atgcactata ccgataaacc
     7681 aatggctatt tttatgttaa cgcctgatta tgatccatct aaccatatca ttgttagcat
     7741 ctttcttagt cagttgtata aagaattatc tacgcaatgt gttaagacta aaggagataa
     7801 atgtcaccgt aggattcatt ttattttaga tgaatttgga aatatgccac cgatggacaa
     7861 catggaagga atcatgacgg ttacggcagg tcgtaatatg ttatgggatc tgtttattca
     7921 atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta tcaaagaaaa
     7981 ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag aattttctaa
     8041 aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat taggtatgaa
     8101 tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa gaatttctac
     8161 ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt tgaaaggtca
     8221 taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt atgcttatca
     8281 atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac attgtacaca
     8341 tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac cagatgaata
     8401 tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg aagaaaatat
     8461 gttttataaa atcgttcaat cttttgagaa tgagacggct aaaaaattgt taattcaatt
     8521 ttacgaagaa caagcaacgg atcgtcttca agaatatgtt gaaaagttga tcaaaaatga
     8581 agaaatttct gaaatgatta ggaaacggct gttattggaa gctgaaaagg cggtgaaaga
     8641 atgatcattg gaggaatttt ctctggcata gccgactggt ggtcaggttt attcggagaa
     8701 ggggatgcag atcccaaaac cgtaacgaaa gtcttacaag attatggtaa ttatctgcag
     8761 tatcaagatg tattcagttg gatttgtaat acttttttat ggggattgat caaagggtta
     8821 tataatctca atactttatt agaaaaaaca atttatcaaa gctttgattt aaaagactta
     8881 ttgaacgccg ctggtgttaa tgaactattt caaagtttga ttagtaaggt attggctctt
     8941 ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa acacccaccg
     9001 aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt tggtggtagt
     9061 tctttaatcg atgaagggtt aaatatcagt aaaaactttt atggagacgt cactgcagca
     9121 aataaagcag ataaaacggg ttcacctgct tttcaaatta ttcaaaataa tgtgattgat
     9181 gtcagtacat tactagcgac tgacccgaat aaagtagaat ccttaccaac agataaaaga
     9241 aatgctttaa cagcgaaaaa tttcaaaaca gcgaatatca atgggattat tacacctgat
     9301 caagctaaga aaatgtctga atcaagtgat attagtacag tacaaaaaga tcggttaaag
     9361 gcattacagt atcgactaga gttagacgat aatgggaaag aaatccctgt tcaaattagt
     9421 gatgaaggtt tagcaaaata tgtgtatacg tcaggctatc gtcgatacat tagtagtcca
     9481 gggattattt tggctgggga atcttctttg tcggtagcct atttatttat tttgtttaca
     9541 atcgttactt gcattattga gctagtattt aaaaaattct atttagtaat tgccgcgtca
     9601 acggactttg agacaggaca acggatgaaa agtgcgatag aagatatttc tcaatccttc
     9661 ttgttattgg cgtttacgat tttagaatta cgaatctata cattaatgct ttcaggtatc
     9721 ggtgatttgc atgcggtagg aaagatcaat ggattcttgt atgttgttgc tttaattgtg
     9781 ctaactattg cgctattcaa aggctctcaa tcggttacga agattttcgg tgttgatacg
     9841 agtctgaaaa acggcagcaa ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa
     9901 aatgtgggta gcggagctaa aaaccttgca agtgctggta agagtggttt aagccattta
     9961 aatgatattc gtaagaatgg atttggccat cgaaaagata ttacccatga taacggggaa
    10021 gaacaatcag acgataaagc tacaactgta cctaatagta agaagacagt ttccgaaatg
    10081 ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa
    10141 cgtgggttaa aaggaacagt tgaagatctt acagaaaaag ccaaagacac cttagaagag
    10201 aaattagacg gcacgagagc taaagaattt gctgaagctg tgaagaatcc atccgaagca
    10261 ctagaaaaac aaaaagaaaa agcttcagca gtcaaagaag gactaaaaga tgcgtatggt
    10321 gaaggacaag tcaatgcagc agtgaaggca aatacaacaa gtgaaaaaga aaatcaagaa
    10381 ggtgcagcga ttccttcagc caaagatcac caagatgagt tacattctaa cgaaaattcg
    10441 atcggagaaa aagattcaga agaaggacaa catggtcagt taccaaaaac cgatccagaa
    10501 caatatcgtt ataaatctaa cattaattct gaaaacgaaa aacgcatttc agatttacca
    10561 aaacgaaatc aagaagtaca aacggatgag gattccatcg atctgccaaa tgataatggc
    10621 atcgaagttg gaactgaaaa atcaacgaaa attggtttgc ctaagacaca aaatttacca
    10681 atgaaagata gtcgtttgaa tcaattcaaa gagaatgatc aaagctttgt tcatcaatca
    10741 cctgcaggta ctgttcaaat gaaatcaagt cgtttgcctg aaaatggaaa tctgacaact
    10801 gtttcttaca atttagctga aagtggtgaa acagagaatg cgtccgttca aaatattgtg
    10861 acaaaagaag ctgcacctaa gccaatcaat aaagttcata atggatacag accagcaaac
    10921 gtagcattta ctgcaccgac aaaaataaat gtgacaagag aaactaaaaa caggaacaat
    10981 gaattgaaag aacaactatt gaaggatatc aaaccataaa aggcctattt aagggccttt
    11041 ttctttataa aacaagttta aaatggaaag gagaaaattg tggaggatat tttaatagct
    11101 acgtttataa ctgcactggg agcattttta acgatatttg tggtgactat taaaggattt
    11161 aaagatacgg atagtttaca aaaagatcat cagtctattt tagatggtca aaaaacacat
    11221 accgaaagca ttattgtaca cctttcagaa ggattcactt atttgaaaga tgctatgccc
    11281 acattcacag taaaattaac ggaaatattt cattctgttg agcaaatatc gtcagaattg
    11341 aaagagcaaa gacaacaaaa agatcgtcaa cttggctcac ttaattttag gcaagaaacg
    11401 atacaagaat cattggagaa tgtaaatcga ttaaacgatg aaatgcaacg agttcatttg
    11461 gaaaatgaag aattacgcgc acaaaattat gatttaattc aaaagaaaag aaatctggaa
    11521 caaaaaattg ttgaattaaa agaagaaaat cgtcaattga attatcaatt acgacgagga
    11581 aaaaatcgtt ctcgtgattt aggaaatgaa ttagagttgt aatatttaga aaaacgctgt
    11641 gagaaatcac agtgtttttt atttctaaaa atacaaaata taaggggtat ggttatatta
    11701 tggataaata cattcatatg aaaaatcctc caagatttag tggggaatgg aagtttttcg
    11761 cctttttctt tttagatcta ttaacgcttg gtggctgctt tttttgggcg aaaatagtca
    11821 ataatcttgt ccacttacca ctcttttttg cactcttgaa ttatggatta tggcttgcga
    11881 tagcgttagt gattgtttgg cgaccctata ataaccctgg tcaacgccaa atttttgcga
    11941 ttatcgaatc tattttcttt acggataaga accactatca tgcgttagat cctaaccgaa
    12001 attatcatgg tacgtataag gaggatgaat aaatgcgagt caaaggacaa aattcagttg
    12061 ataagaaatc cattttgcag tttggaaaac cagaagagcg ctctatcttg gacttatttc
    12121 catataaatc ctgtacagaa gagggagtct taattactag agaagatcat tttcagcgtt
    12181 attatcgtgt cgtttccact gacgtggagg gattaaatga acaagagaaa ctagaacgaa
    12241 tgaatcagtt gaccaccgtc atgcgtacgt atgtgccaac aattaagtta gttagtttga
    12301 ccacagagac ggatctaacg gaacaaatcg tgcaaaagcg gcagttatta gataaaaatc
    12361 gtatggaaca gtcactagga agaaatctac agcgattaag aaaatatgaa aagaaattag
    12421 tagaggagat ccaggaatta aaacgggcag aacaagaacg tcctgactta agcttctttt
    12481 tcgttattga aggaaagaca atcaaggatt tacaaactag aaatcgtcag ttaatgcgat
    12541 ctagtggcat tttagggtta aaaccactct acaaaaaaga attgattaag attctttatc
    12601 gaatgaacaa catgaatgac gaataaaaaa gatggaggaa atgattcatg ctagatgtga
    12661 taaaaacagc tttttcaacg caaagtaatc aaaaggtgat tgatccatta aaacaacttg
    12721 gtaatcgtga aatagagatt ttaaaatcaa aaggttatga tttagatttc ttaaaacaag
    12781 tgatgccaca aggtgggttg aattttgatg aagaatatgt gacctatgga aatggttatt
    12841 cacgatgtgt gacagtttat cattacacaa aaaatcctac tcttttttgg ttggccaatc
    12901 tcatgaacaa tcctcataca gtggccacac tagatgttag cacagatgaa aagcaaaaag
    12961 tgattgatca aatcaaccgc gcattagatg aattaaatga tcgatcagat aatgaacgaa
    13021 aagcgactga ccgaaataaa agtgcggaag aatacctgga actagcggaa tatgcccgaa
    13081 aattgacttc taatggggaa atttctaaac aagtcaaagt gagagtacat gtttacggaa
    13141 atacgatcca agaagtggat gaacgggcgc aggaactgat cgaaaatcta aaagggatgg
    13201 attatcgtgc ggtgacttat ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca
    13261 tgttcgatga acaagctgaa tggtttggaa atatcaaagg gcaatcactt ccatcgttaa
    13321 acattggcgg aggattcccg ttccaccacc aaacgctaaa agatccagga ggcacacaat
    13381 atggggaaac catgacaggc ggcccatttg tctggaatgc cacgtataaa aatagtgtac
    13441 gtttatctac caataccttg cttctaggaa tgatgggagc tggtaaatcg accgttctta
    13501 aaatgatcgc ggaagcccat ttagctgcag gagactttat ttggggcttt gaaaagggaa
    13561 aagactttat tcctttctta aaggaatata acggtattat ggtgcgttta gacggatctg
    13621 acgggatgat caatccttta gagatctttg ccacacggac gtatgatgaa gcatccagtt
    13681 tatatgacga tggttcagtc aaagatttga agatcaatga agcagcgtcc tatcaaacgc
    13741 acttagacaa agtagtctat caggtacagt tagtgagtcc tcagctaaaa ggaacgatga
    13801 aagcagaatt caagacctac ttgaatcgtt tttatgaaga gtatggaaca gtaccacgcg
    13861 gttttacttc ctcaaattcg cgttcaaata ctgatacgca agtcacaggg aaagatccag
    13921 aagcttatcc gacgttcaaa gaatttttag cctttttagg acaactggaa ctaccagggg
    13981 cttcccaaga gaaaaagaat cggaaagaag aaatggagtc tatcgtggaa tccttatgtg
    14041 agacttatgg catgattttt gatggtcata gtacgattcg acaccttgac caacaacagt
    14101 tggtcttttt tgacattgac tctattacag gcctagatcg aggggtacaa caatgtcaga
    14161 tgtacatggc catgacgttg atttggaatc aggcattgat tcaaggacga agacaacgac
    14221 gctta
//
LOCUS       DAFEZB010000069        14115 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFEZB010000069
VERSION     DAFEZB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.069"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..552)
                     /locus_tag="LP859_002203"
     CDS             complement(<1..552)
                     /locus_tag="LP859_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL3030131.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
     gene            complement(568..1047)
                     /locus_tag="LP859_002204"
     CDS             complement(568..1047)
                     /locus_tag="LP859_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL3030132.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1053..2077)
                     /locus_tag="LP859_002205"
                     /pseudo
     CDS             complement(1053..2077)
                     /locus_tag="LP859_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2094..3200)
                     /locus_tag="LP859_002206"
     CDS             complement(2094..3200)
                     /locus_tag="LP859_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3030133.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDCLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="LP859_002207"
                     /gene_synonym="adhC"
     CDS             complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="LP859_002207"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL3030134.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="LP859_002208"
     CDS             complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="LP859_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL3030135.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="LP859_002209"
     CDS             complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="LP859_002209"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL3030136.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8171..8869
                     /locus_tag="LP859_002210"
     CDS             8171..8869
                     /locus_tag="LP859_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL3030137.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9037..11676)
                     /locus_tag="LP859_002211"
     CDS             complement(9037..11676)
                     /locus_tag="LP859_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3030138.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12295..13326)
                     /gene="queA"
                     /locus_tag="LP859_002212"
     CDS             complement(12295..13326)
                     /gene="queA"
                     /locus_tag="LP859_002212"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL3030139.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13519..>14115
                     /locus_tag="LP859_002213"
     CDS             13519..>14115
                     /locus_tag="LP859_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3030140.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3958 a         3095 c         2543 g         4519 t
ORIGIN      
        1 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
       61 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
      121 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
      181 accaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
      241 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
      301 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
      361 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
      421 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
      481 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
      541 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
      601 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
      661 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
      721 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
      781 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
      841 tacggctaaa cgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
      901 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
      961 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     1021 taataccttg tttaaaatca ttttcattac agctacgctc cttgctcttg gatcttttga
     1081 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     1141 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     1201 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     1261 gcttcttcaa tggctttttc ctgttcatca gttagctgtt ctttgatggc atcattcatg
     1321 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc taccccgcca
     1381 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     1441 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     1501 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     1561 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     1621 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     1681 atcaccttaa agaaatgttc ttcatcattg aatagataag gaacggaaaa aatcgaataa
     1741 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     1801 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     1861 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     1921 actggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttacg tctgcatctg
     1981 cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga cctaggctaa
     2041 gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt cttttaacaa
     2101 taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata aggcattgct
     2161 gtgattccga tattccgata aaaatctaaa cagtccccca ttcctgtccc gattgaaacg
     2221 ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc acgataagcg
     2281 acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat accgtaagca
     2341 aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa cgtatgccat
     2401 aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag tgtttcgatc
     2461 aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat ctctggaata
     2521 gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt atgatttcct
     2581 tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta tttgatatgg
     2641 atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag tttcccgatt
     2701 tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt cttattttcc
     2761 ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc attcatgacc
     2821 cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag
     2881 taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc gccttcttcc
     2941 atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt tgtcatctgg
     3001 taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac tgtccagctt
     3061 ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc gtctttgatc
     3121 gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt aggtacaaaa
     3181 atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt ttatatcctt
     3241 aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac tgtctaaaat
     3301 tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa aaaaagaact
     3361 tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt ttctacgctt
     3421 tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga gatcaatggt
     3481 tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat acggtctacg
     3541 gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt gatgccgaca
     3601 gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc tgttgtttcg
     3661 cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg gaaatagtca
     3721 tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat aatatgcggt
     3781 aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat tttatgtgcg
     3841 actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat tgtagaagcg
     3901 ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc taaatagtca
     3961 aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc catgactgaa
     4021 acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt gacagatgct
     4081 ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa tgcataatct
     4141 gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg cgttacttca
     4201 gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg tttgtcgatc
     4261 ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa agatgtgtct
     4321 gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc gcctccaaga
     4381 gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta tacggtattt
     4441 gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt tttacgtcgt
     4501 gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg gtcacaaacg
     4561 atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt cttttcaaag
     4621 aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc tacagttttg
     4681 acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc ataagaaccg
     4741 catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc gccttctgca
     4801 gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt gacatgcaac
     4861 tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt caacatacct
     4921 tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag aactggagaa
     4981 agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc aatcagcatt
     5041 ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc agaatgtcca
     5101 acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc taatttttgc
     5161 aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt tactgctgca
     5221 tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc attatcaaat
     5281 gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt ttcaatataa
     5341 gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta agcagacttg
     5401 accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt cattagcata
     5461 gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg agctccggca
     5521 gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg ggcacttgga
     5581 tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt gaaaatcgtt
     5641 gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg ttcagcaatt
     5701 tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc atgtttgata
     5761 ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc gtaaacaccg
     5821 cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa agctgccatt
     5881 gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat ttctttcaat
     5941 gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat tgtcttcgtt
     6001 ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg ttatttgtta
     6061 aataattcac atgtatatca tacaatattt atttttctat gtaaacactt ttttgtgatt
     6121 tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg tatagaaaaa
     6181 tatataaagt tatttattat tatttatatg acacacccga ttattcaata ttgagcaata
     6241 tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac atctttctgc
     6301 cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt ctgtctcttc
     6361 cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt ctgttgtttc
     6421 aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt tgattgctgc
     6481 acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc
     6541 gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac ctggtttcat
     6601 gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa
     6661 ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca tttttttcac
     6721 ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata acactagttt
     6781 tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt tattgaaccc
     6841 gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga ttgcttgacg
     6901 tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca aacgaatccc
     6961 aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt ttttacatgt
     7021 ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg catttttcac
     7081 tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag taggcaagac
     7141 acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag cagcaccgac
     7201 acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt ctaatacacg
     7261 attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac cagggaaatc
     7321 catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg ctccttggat
     7381 gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc gttctgccca
     7441 tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga atggtggaca
     7501 ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga ttgctttttc
     7561 tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc
     7621 gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg
     7681 ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt cttcaccagg
     7741 gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt ctttcaactc
     7801 ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg
     7861 gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt tttctttctt
     7921 gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct atataaaata
     7981 tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc cctcggcttc
     8041 cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca aaaaaaagaa
     8101 aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta aagaaaagga
     8161 gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga gtctaaaagg
     8221 acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta ttgctactat
     8281 tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg gatcagcgag
     8341 cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg gaattatttc
     8401 cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg atattttacg
     8461 tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca aaggtttgtt
     8521 tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat tatggggttt
     8581 gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct attttattta
     8641 ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga tcactgcaag
     8701 tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt catttatcgg
     8761 ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca gcccatacat
     8821 ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa agagagtgtg
     8881 acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta aaagaaacat
     8941 tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct ttttgtatag
     9001 ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt ctgcttccgg
     9061 taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata aaatccctac
     9121 caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg taaatggcag
     9181 caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca aagctttatt
     9241 ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca catgcagaat
     9301 cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac ttgcgtctac
     9361 ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg ttacgatggt
     9421 gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga tcccttcatt
     9481 tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc ctaaagcaaa
     9541 tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg taaatggtgc
     9601 tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat tacagcttag
     9661 caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac tgacagcgtc
     9721 tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt gcgccacatc
     9781 tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg gtgcatcatt
     9841 cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt tgacgatccg
     9901 gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac gcgcatctaa
     9961 ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg gatcattcaa
    10021 gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag tgatcatgac
    10081 tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg gacgtggtgg
    10141 atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt cagaagaaat
    10201 atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca gtgcccgttt
    10261 accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa agcgcggagc
    10321 aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt ggtgaactgt
    10381 tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat atttttcttc
    10441 taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta cttctgttgg
    10501 attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg tacatagtgc
    10561 agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat tcgttacttc
    10621 atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg ttcctgtttt
    10681 gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact gtcggatgat
    10741 cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa caataacagt
    10801 caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca tctcaaggag
    10861 tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta aagcgatcat
    10921 actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt gcatagtatt
    10981 ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg cagtgacgat
    11041 cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa gttggtcacc
    11101 taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt cactttctcc
    11161 agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg catcggcaga
    11221 aatagcatca ccggactcta aaacaagaat atcacccact accagttgct ctgactcaac
    11281 agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat cttgcaagga
    11341 ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt tgatcacaac
    11401 gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa atgcggtata
    11461 aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca agcttcgaaa
    11521 gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc cctgcttttt
    11581 tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt ctgttacctt
    11641 ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt aaaatcttcg
    11701 tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc aaataaaggg
    11761 cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg caaacgctac
    11821 caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga cagaagaatt
    11881 tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca cccagacatg
    11941 cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc acgaagtatc
    12001 cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg aaaattgaat
    12061 aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt tgtttaacat
    12121 gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat ttctgtttat
    12181 ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt tttctttgtc
    12241 ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat aaccttattt
    12301 cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg catgctggta
    12361 agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta aagtcgattt
    12421 tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc caggcgtaat
    12481 gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg ttccaatcgt
    12541 ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat ttttacgtac
    12601 ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat gcatatggtg
    12661 ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat gtagtgtcag
    12721 atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg taaaatgcaa
    12781 tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt gatagcgatc
    12841 tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca atgattcaag
    12901 aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt gttccaattc
    12961 ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc caactttggc
    13021 acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg ttagaagaag
    13081 gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag gcagaactcg
    13141 tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt catggaaatg
    13201 tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat gatcgcggtc
    13261 ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat cttctgttgt
    13321 taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt ttttcgttct
    13381 gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa aaaaactacc
    13441 ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt cattgaaaat
    13501 agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta taaaggtgga
    13561 aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat ttgttttatc
    13621 ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat gactgatcca
    13681 acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc agtggaattg
    13741 cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat gacgatccgt
    13801 gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg gaataaaaca
    13861 gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg ctacccacat
    13921 gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc agcgaatcaa
    13981 gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg tgattccttg
    14041 caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt tgtaacccat
    14101 gacatggatg aagcg
//
LOCUS       DAFEZB010000070        13802 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFEZB010000070
VERSION     DAFEZB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.070"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(21..251)
                     /locus_tag="LP859_002214"
     CDS             complement(21..251)
                     /locus_tag="LP859_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030141.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LP859_002215"
     CDS             complement(687..1253)
                     /locus_tag="LP859_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3030142.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LP859_002216"
     CDS             complement(1253..2209)
                     /locus_tag="LP859_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL3030143.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LP859_002217"
     CDS             complement(2209..2865)
                     /locus_tag="LP859_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL3030144.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LP859_002218"
     CDS             3235..5253
                     /locus_tag="LP859_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3030145.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LP859_002219"
     CDS             5680..8094
                     /locus_tag="LP859_002219"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL3030146.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LP859_002220"
     CDS             8442..9995
                     /locus_tag="LP859_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL3030147.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LP859_002221"
     CDS             complement(10376..12160)
                     /locus_tag="LP859_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030148.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..>13802)
                     /locus_tag="LP859_002222"
     CDS             complement(12175..>13802)
                     /locus_tag="LP859_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030149.1"
                     /translation="PKLVAKLIDTGIASGEASVIKKIGVEMLLVAFLGTIAAVASSYF
                     SALASARFGFQTREKYFRKFQQLSMKDISRFSSGSLLTRMTNDVDNVQQMIVLFCQMI
                     LPAPVICVFTILMMFRYSLLLTWVTLISVVFYVWIVYRLMKRGAPLSLSIQPKMDRIT
                     ITLREFFTGINMIRAFDNQSYEEKRTNKSFADYANQMIKVNRIFAWVTPIAFLLMGIV
                     YASILWFGGNLVAEGALQIGVVTAVVEYSMLTLAYLMIAAMVLVIIPKSFASLRRIEE
                     ILQAEIEIEEETIKTVAEPTFDQTNAAAFEHVTFKYTEISDPVLEDIDFTIPKGKTTA
                     IVGGTGSGKSSLAKLLLRLSDPTIGKVTLGGIPLTQMTQEQIRSHISYVPQKAFLFSG
                     TILSNLQMGNEYASTEELSKAIKIAQLEELVDTLPDGLDSFVAQGGSNYSGGQKQRIC
                     IARALIKPAEIYVFDDSFSALDYKTDAALRAALHKHMSEKTLVIVAQRLSTIQQADNI
                     IVLDEGKIVGQGTHEELLSNCQTYQEFAKSQGI"
BASE COUNT         4416 a         2621 c         2710 g         4055 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 ta
//
LOCUS       DAFEZB010000071        13773 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFEZB010000071
VERSION     DAFEZB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.071"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..1578)
                     /locus_tag="LP859_002223"
     CDS             complement(<1..1578)
                     /locus_tag="LP859_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL3030150.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSVEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRL"
     gene            complement(1741..2334)
                     /locus_tag="LP859_002224"
     CDS             complement(1741..2334)
                     /locus_tag="LP859_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030151.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2335..2667)
                     /locus_tag="LP859_002225"
     CDS             complement(2335..2667)
                     /locus_tag="LP859_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030152.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2719..5097)
                     /locus_tag="LP859_002226"
     CDS             complement(2719..5097)
                     /locus_tag="LP859_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030153.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMTDKDKSSLSDLQKDRLRALQYRLELDDNGKEVPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNI
                     NSENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRL
                     NQFKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREA
                     APEPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            complement(5094..7706)
                     /locus_tag="LP859_002227"
     CDS             complement(5094..7706)
                     /locus_tag="LP859_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL3030154.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYRYVPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDTKGSFPGYGGIPITHYKKYWYIQTDTVNTCIIGTSRSGKGQIEVLATIDNLSRAE
                     KQSSLVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYTKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIGVEFYHDGKK
                     FHEERVKVGYKEFCEINFDCHLEQGDQVCLKYDHQGKEYVAWYEFSQKVLKDEKGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYKQLRAKYGEDNADIIKENCQTHIYIMSSNTDTIEEFSQNVGNKTVEQENST
                     INALGMNTHINRNVDSDRILPPERIRSLLKGETIVLAPLKREDLKGNKIRPYPIFNSE
                     KTNSPYAYQYLEDFDTLGRTIHDIDIYCTHADLDLKALQIDYDYFLPDEDARDLYHGL
                     HEQLEEKIEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNEEISEII
                     GKRLLLEADKAVRE"
     gene            complement(7706..9763)
                     /locus_tag="LP859_002228"
     CDS             complement(7706..9763)
                     /locus_tag="LP859_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030155.1"
                     /translation="MYFTIAGNTKKDKELIKKATKEVGKRTSFLPKTMKYSVIETEEQ
                     LHVLGKVLEQESQSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     DESKEKETDNVDEQADIPTQKSIFEEENEVMDQLDGDPFEETMEETTEVEIDPVEANE
                     EPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNQLFLNE
                     LYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEERQQ
                     ETDQEINEYAAEQSSNWEKAKEELNKEEQQVIEEEVQKIKERYEKKRDNAYSLCVEKI
                     NNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTE
                     VLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLA
                     EQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQLQPMPSVAAVQTPMNN
                     QTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQ
                     EQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKTKEGSSDSTVQSNSSESSESN
                     EVK"
     gene            complement(9832..10134)
                     /locus_tag="LP859_002229"
     CDS             complement(9832..10134)
                     /locus_tag="LP859_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030156.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGIILAAASIVVSLFGMFGQTPQGL"
     gene            complement(10152..10352)
                     /locus_tag="LP859_002230"
     CDS             complement(10152..10352)
                     /locus_tag="LP859_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL3030157.1"
                     /translation="MAKAKTPFEKAASKILERRGVSETQWKQEVLEKAQFDLLRGKDK
                     DLEGYIYQREREKLVFDEIIKH"
     gene            complement(10392..10859)
                     /locus_tag="LP859_002231"
     CDS             complement(10392..10859)
                     /locus_tag="LP859_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030158.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLVNNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGISDFI
                     NKPDSLFIVVPYEKDGVKKMVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            11048..11254
                     /locus_tag="LP859_002232"
     CDS             11048..11254
                     /locus_tag="LP859_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030159.1"
                     /translation="MYADKNEIEWLLFESGISVKDIYMESGVARTTVIDLINKTSSID
                     KMRFDNAVKLTKMAKMRKNLYENK"
     gene            11394..13460
                     /locus_tag="LP859_002233"
     CDS             11394..13460
                     /locus_tag="LP859_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL3030160.1"
                     /translation="MKLLNPKTVIAGTFAVTLAATLSLAGSVDAASTSVMYRLYNPNN
                     HEHFYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEV
                     KWLTKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWK
                     YEGIGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDS
                     KATQKQVDSAKNTLDSAYKGLKKKPAPTVNKAELQALYNRLKSTAKGDFTEESWNNFQ
                     TALSNAKKILDDSKASQAQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLL
                     KEEHVSVKAGTSYTAKAKTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVE
                     VKARPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPTFTNFVLDPRESSSNTVTLN
                     NVTSNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDNFSTSWKNMTDQNYFL
                     CRNDDSSVTVDKNGQRIINHVDQNRTITFKYKNISLNDLNNYVRQKELAWLNSYRQQN
                     GVAPMQFNDVVQKAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYL
                     YVDEILSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFIS
                     ANPIFK"
     gene            complement(13496..>13773)
                     /locus_tag="LP859_002234"
     CDS             complement(13496..>13773)
                     /locus_tag="LP859_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030161.1"
                     /translation="FLSNNLLDGREIQLIFTAKTPLQSEKNIDNQAVVAVDNVSNKTN
                     VVTIGVDPNLPQVIVPKTGSTHLVTTLVVSLLLLVLATFSYVVLKFN"
BASE COUNT         3952 a         2789 c         2200 g         4832 t
ORIGIN      
        1 taaacgtcgt tgtcttcttc cttgaatcaa tgcctgattc caaatcaacg tcatagccat
       61 gtacatttga cattgttgta cccctcgatc taagcctgta atagagtcaa tgtcaaaaaa
      121 gaccaactgt tgttggtcaa ggtgtcgaat cgtactgtga ccatcaaaaa tcatgccata
      181 cgtctcacat aaggattcta cgatggattc catttcttct tttcgattct ttttctcttg
      241 ggaagcacct ggtaattcca gttgtcctaa aaagtctaaa aattctttga acgtcggata
      301 agcttctgga tctttccctg tgacttgcgt ctccgtattt gaacgcgaat ttgaggaagt
      361 aaagccgcgt ggtactgttc catactcttc ataaaaacga tttaagtagg tcttaaattc
      421 tgctttcatc gttcctttta gctgaggact cactaactgt acctgataga ccactttgtc
      481 taagtgtgtt tgataggacg ctgcttcatt gatcttcaaa tctttgactg atccatcgtc
      541 atataaactg gatgcttcat catacgtccg tgtggcaaat atctctaaag gattgatcat
      601 tccgtcagat ccgtctaaac gcaccataat accgttatat tcctttaaga aaggaataaa
      661 gtcttttccc ttttcaaagc cccaaataaa gtctcctgca gctaaatggg cttccgcgat
      721 cattttaaga acggtcgatt taccagctcc catcattcct agaagcaagg tattagtaga
      781 caaccgtaca ctatttttat acgtcgcatt ccagacaaat gggccgcctg tcatggtttc
      841 cccatattgt gtccctcctg gatcttttag cgtttggtgg tggaacggga aacctccgcc
      901 aatgtttaac gatggtagtg attgcccttt gatatttccg aaccattcag cttgttcatc
      961 gaacatggta aacatcgatt gccaatctcg tttggtttta tgcagataag tcaccgcacg
     1021 ataatccatc cctttgagat tttcgatcag ttcctgcgct cgttcatcta cttcttgaat
     1081 cgtgtttccg taaacatgta ctctcacttt gacttgctta gaaatttccc cattagaagt
     1141 caattttcta gcatattccg ctagttccag gtattcttcc acgcttttat ttcggtcagt
     1201 cgcttttcgt tcattatctg atcgatcatt taattcatct aaggcacgat tgatttgatc
     1261 aatcactttt tgcttttcat ctgtgctaac atcgagtgtg gccactgtat gaggattgtt
     1321 catgagattg gccaaccaaa aaagcgtagg attttttgtg tagtgataaa ctgtcacaca
     1381 tcgtgaataa ccattaccat acgtcacata ttcttcatca aaatttaacc caccttgtgg
     1441 catcacttgt tttaaaaaat ctaaatcata ccctttggat tttaaaattt ctatttcccg
     1501 atttcctagt tgctttaatg gatcaagtac tttttgatca ttttgtggtg aaaaagctgt
     1561 tttaattgct tctaacatgt acagtcacct cttcttgttt gattctttac ttggttaaaa
     1621 gattgccctt ttcccaactt acccaggctt aaagtgtgag gaaattttct agtggtctct
     1681 tccttcggaa catctttttc taaccatatc aacggatatt tcattctctc ttctccattc
     1741 ctactcatca ttcatattgt tcattcgata cagaatcttg atcaattctt ttttgtagag
     1801 tggttttaac cctaaaatgc cactagatcg cattaactga cggtttctgg tttgtaaatc
     1861 cttgattgtc tttccttcaa tgacgaaaaa gaagcttaag tcaggacgtt cttgttctgc
     1921 tcgtttcaat tcctggattt cctctactaa tttcttttca tattttctta atcgctgtag
     1981 atttcttcct aaggattgtt ccatacgatt cttatctaat aactgccgct tttgtacgat
     2041 ttgttccgtt aaatccgtct ctgtggtcaa actaactaac ttaattgttg gcacatacgt
     2101 acgcatgacg gtggtcaact gattcattcg ttctagtttc tcttgttcat ttaatccttc
     2161 cacgtctgta gaaactacac gataataacg ttgaaaatga tcttctttag taactaagac
     2221 tccctcttct gtacaggatt tataaggaaa taaatccaag atagatcgtt cttctggttt
     2281 tccaaactga aaaatggatt tcttatcaac tgaattttgt cctttgactc gcatttattc
     2341 atcctcctta tacgtaccat gataatttcg gttaggatct aacgcatgat aatggttctt
     2401 gtccgtaaaa aaaatagact cgataatcgc aaaaatttgt cgttgacctg ggttattata
     2461 gggtcgccaa acaatcacta gagctatcgc aatccataag ccataattca agagtgcaaa
     2521 aaagagtggt aaatggacaa ggttattgac tattttcgcc caaaaaaagc aaccaccaag
     2581 cgttaataga tccaaaaaga aaaaggcaaa aaacttccat tccccactaa atcttggagg
     2641 atttttcatg ttaatatatt tatccataaa tgatttctcc ttttcataca tagaaaaaag
     2701 gcctaacttg taggcctttt atggtttgat atcctgtttg atatccctca atagttgttc
     2761 tttcaattca ttgttcctgt ttttagtttc tcttgtcaca tttatttttg ttggtgcagt
     2821 aaatgccaca tttgctggtt tgtaacctgt gtgaactctt tgaataggtt ctggtgcagc
     2881 ttctcttgtc acaacatttt gaacggacgc attccctgtc tccccacttt cagataaatt
     2941 gtaagaaaca gttgtcagat ttccattttc aggcaaacga cttggtttca tttgaacatt
     3001 acctgcagtt gattggtgaa caaagctttg atcattctcc ttgaattgat tcaaacgact
     3061 atctttcatt ggtaaatttt gtgccttagg taaaccaatt ttcgttgatt tttcagttcc
     3121 aacttcgacc ccattatcat ttggcagatc gatggaatca tcatccgttt gtacttcttg
     3181 atttcgtttt ggtaaatctg aaatgcgctt ttcattttca gaattaatgt tagatttata
     3241 acgatattgt tctggatctg tttttggtaa ttgaccattt tgtccttctt ctgaattttc
     3301 gttagaatgc aactcatctt ggtgatcttt ggctgaagga atcgctgcac cttcttgatt
     3361 ttctttttcg gttgttgtat tagcttttac tgctgcattg acttgtcctt caccatacgc
     3421 atcttttagt ccttctttga cttctgaagc tttttctttt tgtttttcta gtgcttcgga
     3481 tggattcttc acagcttcag ccaattcttt agctctcgtg ccgtctaatt tctcttctaa
     3541 ggtgtctttg gctttttctg taagatcttc agctgttcct tttaacccac gttcattgac
     3601 gtaaccaaga ccttctgcgg ctttagaaac accatttttc gctttcccga acatttcgga
     3661 aactgttttc ttactattag gtacagttgt agctttatcg tctgattgtt cttccccgtt
     3721 atcaggggta acatcttttc gatggccaaa tccattttta cgaatatcat ttaaatggct
     3781 taaaccactc ttaccagcac ttgcaaggtt tttagctccg ctacccacat ttttcgcaat
     3841 atttcctaag gcaaacatgc taagaagaga attgctgccg tttttcagac tcgtatccac
     3901 accgaaaatt ttcgtaactg attgagagcc tttgaataac gcaatggtta gtacaattaa
     3961 agcaacgaca tacaaaaatc cattgatttt tcctgccgca tgcaaatcac cgatacctga
     4021 aagcattaat gtatagattc gtaattctaa aatcgtaaac gccaataaca agaaagactg
     4081 agaaatatct tctatagcac ttttcatccg ttgtcctgtc tcaaagtccg ttgacgcggc
     4141 aatcactaaa tagaattttt taaataccag ctcaataatg caagtaacga tcgtaaatag
     4201 gataaataaa taggctaccg acaaagaaga ttcaccagct aaaataatcc ctggactact
     4261 aatgtatcga cgataccctg aagtataaac atatttggct aagccttcat cactgatttg
     4321 aacaggaact tcttttccat tatcatccag ttccagtcgg tattgtaacg ctcttaaccg
     4381 atccttttgt aaatctgata aactactctt atctttatcc gtcatttctt tagcttgttt
     4441 aggagtaata atcccattga tattcgctgt tttaaaattt ttcgctgtta aagcatttct
     4501 tttatctgtt ggtagggatt ctactttatt cgggtcagtt gctagtaatg tacttacatc
     4561 gatcacatta ttttgaataa tttgaaaagc aggtgaaccc gttttatctg ctttatttgc
     4621 tgcagtgacg tctccataaa agtttttact gatatttaac ccttcatcga ttaaagaact
     4681 accaccaaaa atcaagatca tggccataaa taaattaacc aagatatttt tgacttttgg
     4741 tggatgttta ctcataaaaa atttcaagcc taaataaatt aatgtgacga cacaaaaaag
     4801 agccaatacc ttactaatca aactttgaaa cagttcatta acaccagcgg cgttcaataa
     4861 gtcttttaaa tcaaagcttt gataaattgt tttttctagt aaagtattga gattatataa
     4921 ccctttgatc aacccccata aaaaagtatt acaaatccaa ctgaacacat cttgatactg
     4981 cagataatta ccataatctt gtaagacttt cgttacggtt ttgggatctg catccccttc
     5041 accaaataaa cctgaccacc agtcggctat gccagaaaaa attcctccaa tgatcattct
     5101 ctcaccgcct tatcagcttc caataacaac cgtttcccaa ttatttcaga aatttcttca
     5161 tttttgatca acttttcaac atattcttga agtcgatctg ctgcttgttc ttcgtaaaat
     5221 tgaattaaca atttttttgc cgtctcattt tcaagagatt gaacaatttt ataaaacata
     5281 ttttcttcga ttttttcttc taattgttca tgcagtccat gatagagatc tctagcatct
     5341 tcatctggta agaaataatc ataatcaatc tgtaatgcct ttaaatcgag atctgcgtga
     5401 gtacaataaa tatcgatatc atgaatggtt cgtcctaatg tatcgaaatc ttcaagatac
     5461 tgatacgcat aaggactatt tgttttttca gaattaaaaa taggataggg tcttatcttg
     5521 tttcctttaa gatcttcacg ctttagcggt gcaagaacaa tggtctctcc ttttaaaaga
     5581 ctcctaattc gttcgggagg caaaatacga tctgaatcaa cattacgatt gatatgtgtg
     5641 ttcattccca aagcattgat cgtactattt tcttgttcca ctgttttgtt gccgacgttt
     5701 tgagaaaatt cttcaatcgt atctgtattt gaagacataa tataaatatg tgtttgacaa
     5761 ttttctttga tgatatctgc attatcttca ccatatttcg cacgtagttg tttatatgat
     5821 tgaataaaca gatcccataa catattacga cctgccgtaa ccgtcatgat tccttccatg
     5881 ttgtccatcg gtggcatatt tccaaattca tctaaaatga aatgaattct acggtgacat
     5941 ttatcacctt tggtcttaac acactgtgta gataattctt tatatagctg actaagaaaa
     6001 atgctaacaa tgatatggtt agatggatca taatcaggtg ttaacataaa aatagccatt
     6061 ggtttatcgg tataatgcat ccgaatagac tctactagta agttactttt actttcatcc
     6121 agtgtgagaa ctgcttcttt ttcccattct ggcaaatgag tttctcctcg tttttttcga
     6181 tagacgatgt ttcccttttc atctttcaat actttttgag agaactcata ccaggccaca
     6241 tattcttttc cttggtgatc atattttaag caaacttgat ccccttgctc taaatgacag
     6301 tcaaaattaa tttcacaaaa ttctttataa cctactttga cacgttcttc atgaaatttt
     6361 tttccatcat gatagaactc tacaccaata cgttgattta atagactttc gtgtaatttc
     6421 aaatctaaat atttgggaaa accaaccgat ttataatcaa tggttgattg agaagtcatt
     6481 ttcgcaattt tcgttagggt aaatgggttc aatcctgtaa ttgccgtcga taaaatattc
     6541 ccttttgctt tatcagatcc aattttcgtt gctccatatt gtttttttgc tagatttcca
     6601 ggtggcaact gattcataaa ctcatctaaa gcattgagtt gcttaaattc attagtagga
     6661 tcttgataat tgaatccacc aatttctgaa agtagttcaa cgagattatt tggtgttaat
     6721 ttggtgtaat cccctgtttg atccgcttgt tctaacaacg caaaaataag cgcattgaca
     6781 gctttttgcg cattttcgtt gaaatattta ttgtcagaag cattcgcgtc ataataaatc
     6841 gtatttgtaa atgtattcgc tagttgcgta gcggtatcca cgtctcctcg ctgccaatat
     6901 ttcacaatta aagccaaagg attatcactc atggactgta atggttcagt tagattataa
     6961 gcataaacat catagcctct ggcttctaat tcgtctttgc tggctacata caactcattt
     7021 ttaggatcat tgacgaccaa tgaactttgt ttttctgcac gagataaatt atcaatagtc
     7081 gctaatactt caatctgccc tttccctgat cgagaagtgc caataataca ggtattgacg
     7141 gtatccgttt ggatatacca gtacttttta tagtgagtga taggaattcc accatatcca
     7201 ggaaaagaac ctttcgtatc cggtatctga ggatagatct gttggatttc ttttagcgtc
     7261 attaaccgat tatccccttt ttgtccatcg gctaacggac gaaaccacat atccattttc
     7321 aagcccataa gtacagctgg aacaacacaa atcgtagaaa caactaagcg tgtacctaaa
     7381 ctcgttgctg gggtcaaagg attccaaaaa ccagctgaat caaaatgaaa gacctgtggc
     7441 atttcctgta attgaaacag attcactcct tgtaagcttc cttgaatgat ttcaaatcga
     7501 ctgaaagctt caggtattct tgtcactaaa taatctaaag ggagatagac taaaccgcta
     7561 tacattagtt tttgtttgac tcgcttatca tgcttacctg gtaatctttc acctgctggt
     7621 acatagcgat aaaaatgtga tagatatttc tttttttcta aattaaaagg cttgtctttt
     7681 cgtttcgttg tttctcgaat ccgcattatt tcacctcatt tgattctgaa ctttcggaag
     7741 agtttgattg tacagttgaa tcgcttgatc cttctttggt cttactagaa tcactctctt
     7801 tactgttttc catacttgac tctgttgttg actgattggt acgattcaac aattcttcta
     7861 attgttgttc atactctttt cttgcttgtt cctgggcttc ttccttgatt gacgccgttg
     7921 tggctacaga atcagcctgt gaagattgat tgatatgatt aaaagcaaat actgtccctc
     7981 cacctagaag caagcttaaa cttgcaatac atcccaccat ccatccgtta aaggaacgct
     8041 gtggtgaagg agttagattt gtttggttgt tcattggcgt ttgaacagca gctactgaag
     8101 gcattggttg aagttgtgct agataattta attgtgcttg tcgcaatgta tccatttgtt
     8161 cttgacgttc cttacgagca gctgcttctt tttccttttc ttgctctaat cgttgttttt
     8221 ctatcgccat tttttgttcc gcaagatcct gttcccgttt tcttatttct aattgttcac
     8281 gctctttttc tttttgccgt tgtttttctt cttgttcttt caattcttgt tgttgaattt
     8341 catactcttt tttccatttt ggaatttgta cttgtaattc ttgctggatc tgttcgtgtt
     8401 tttccataag aacttcagtt gttgtttgat atagcttatt cagttgctca tttaattctt
     8461 tgctaatctt tagcttatct gtacgcaaac cgcggtagaa ctcttctttt cgttctttaa
     8521 tacgttcttt taacaagttt tctttttctt tcgataacgc ttcttgattc gtcttagtaa
     8581 aattgttgat cttttctaca cacaaggaat aagcattgtc gcgtttcttt tcgtaccgct
     8641 ctttgatttt ttgtacttct tcttcaatca cttgctgttc ttctttattt agttcctctt
     8701 ttgctttctc ccaattggaa gactgttccg ccgcatactc attaatttct tgatctgttt
     8761 cctgttggcg ttcttcaaag gtggctaata gatcttcttg tgcaagctca tgccaatttg
     8821 ttgattggac agactctaac tgttctaaaa gtgtgccttt cccctgtgaa attaattctt
     8881 ctttactctc taaaaaagct tgataggttt ggggtaattg atatttcgaa taaagctcat
     8941 ttaaaaataa ttgatttttt tctttcatcc attcatgctc tggcggatat tgatccaaaa
     9001 tataatcaaa tacagttgcc ttttgtatct cagtttcgtt tttctctacc tcttcactga
     9061 taagtggttc aatatattcc tgagaatccc cttcagtcca tgttggctct tcattcgctt
     9121 caactggatc aatctctact tcggtcgtct cttccattgt ttcttcaaag ggatctccat
     9181 ccaattgatc cattacctcg ttctcttcct caaatatcga tttctgagtc ggaatatcag
     9241 cttgttcgtc aacattgtct gtctcttttt cttttgattc atcctttgga aagattgggc
     9301 gttcccatgg aacttcttcg tttacttcga cttcactcac ttgattcact tttctttggt
     9361 cgctctctag ctcttcttct aacacttgta gcaatttttc atttcgataa gactcttcca
     9421 cactcatggt ttcatccgtt tccttaaaca catcaaggcg gatcaaatcc atcaatgtag
     9481 gatagaaacc ttctgaaatt tgataatcca gcggtccaaa aacgtattct gtaccagctt
     9541 tctctatttt tccttctgtg tcatgtaacg tgagagtcaa cttaacagaa gattgacttt
     9601 cttgttccag tactttccct aacacatgta gttgttcttc cgtttcaatg accgaatatt
     9661 tcattgtttt tggtaaaaaa gaagtccgct ttcctacttc tttcgttgct ttttttatta
     9721 actctttatc ttttttggta ttgcctgcaa tcgtaaagta catgagtatt ctcctttcat
     9781 aaaaaaagga gtaaaaagta catagctact ttttactcct tcattttgtt tttataatcc
     9841 ctgtggggtt tgtccaaaca ttccaaataa cgaaaccaca atagaggcag cagctaaaat
     9901 aattcctacc cccatcaata cataaccgac gtgcatcttc ccgttttcag cacctcttcg
     9961 tccaaataag aagaaaagcc ccccaataac aatggaagca acggccgcaa caccagcaaa
    10021 aatcatcaga ttatttttca aactagttgc ggcagtttgt aatgctctaa aaatatcaac
    10081 cgcagctact actttcggtt gtgttaaaaa attcattgta attgattcaa acataagatt
    10141 gttcgctcct tttaatgttt aataatttca tcgaaaacaa gtttttcacg ttcacgttga
    10201 tagatatatc cttctaagtc cttatctttt ccccgtaata ggtcaaattg tgctttttca
    10261 agaacttctt gtttccactg tgtttcagaa acaccgcgac gttctaagat tttagacgcg
    10321 gctttttcaa atggcgtttt agcttttgcc attgtgattc ctcctttagg ataaataaaa
    10381 aagactagcc atcactcgct agtcttttgt aatttgtatt tatttgattg tgatccatca
    10441 tgaattaatt tctctttcgt tcgtttttca tccttactag atccaagaaa aaccattttt
    10501 tttacgccat ctttctcgta aggaacaacg ataaataaag aatctggttt attgataaag
    10561 tcgctaatac ctgcagaatc tactattttg tatctttcta aacctttata gtctttttgc
    10621 ctcttgactg ttatttgtac aggatctgga tgccatacac tttcagaata ttcccatgta
    10681 gtatcgtcta taattttcaa attattaaca agaccaccta cttcttcttt ccaaacagtt
    10741 cctggttgca ataaactatt atcttcttta gcttccgatt tcttattact tgaacatcca
    10801 cttaaaataa taaaaaatat tgcaaacata gagacaaacc atagttgctt tttcttcatt
    10861 ttattccttc tttcgttgtt ttattttagt atatcaaata acgaaagtag caacaaacct
    10921 cattttgata cacggggaac ggatactcat aatttcccac ctttctatag aaacttttcc
    10981 tcttgatatt gtgatttatt acaaaaaaga gctataataa agaaaaaaat caagagggag
    11041 cattgcaatg tacgcagata agaatgaaat cgaatggtta ttatttgaaa gtggaatctc
    11101 cgttaaagat atatatatgg aatcaggtgt cgcgcgcact acagttatcg acttgattaa
    11161 taaaacctcc tcaatcgata aaatgcgttt tgataatgca gtcaaactca ctaaaatggc
    11221 gaaaatgcga aaaaacttat atgaaaacaa ataataagaa accaatgctt attcaaaagt
    11281 attggtttct tattgtattt taatcggtat accgtcattt actcttgact ttgtcggtat
    11341 accgacatat aattaacttg taaaaacatt taatctattt aggagttgaa aacatgaaat
    11401 tattaaaccc aaaaactgtt atagctggaa catttgctgt cacactggct gccacacttt
    11461 ctcttgctgg atctgttgac gctgcttcaa ctagcgtcat gtatcgcctt tataatccaa
    11521 ataaccatga acatttctac acctctagca caaaagaacg tgatcactta gtgaaaatta
    11581 agtggggtaa ttatgaaggc cccgcttggg aagcaccgac tagcggtggt catcttgtct
    11641 atcgtttata caataaaggc cttcgtgatc accattacac agctagttgg gatgaagtga
    11701 aatggttgac taaaaattac ggctggactt atgaagggcc tgcctggcgt tctgccgaaa
    11761 aaaataataa accgatctac cgccttttcc ttcctggtgt aaaatctggc tcacatcact
    11821 acactgcaag ttggaaagaa gtacaatggt taaccaaaga ctatggctgg aaatacgaag
    11881 gtatcggttg gtatggcgct gacacctcaa aacccaaagt gaataaatcg gctttagaag
    11941 ctctttatac gagcgttaag ggaacgaaaa aaggtgacta taccgacaat acctggaacg
    12001 cattccaaac cgctttaaac aatgctaaga aagtgttaaa tgactcaaaa gcgactcaaa
    12061 aacaagtgga tagtgccaaa aatacattag attctgctta caaaggatta aagaaaaaac
    12121 cagcacctac tgtgaataaa gctgaattac aagctttata caaccgatta aaatcaactg
    12181 ctaaaggaga ttttacagaa gaatcttgga ataattttca gactgcttta tctaatgcta
    12241 aaaaaatact agacgattct aaggcttctc aagcccaagt aaataatgct aaaaatagtc
    12301 ttgattctgc atttaaaggg ttacaacaca aacctaaacc ttcagaatat gcggtaaccg
    12361 tccgtcatat gaatcgacaa actggaacgc tgctaaaaga agaacatgtc tcagtaaaag
    12421 ctggtacaag ttacactgca aaagctaaaa ccgacttgac taactttgaa gcaccacagt
    12481 accaagtaaa tgggaatgaa acacaaacac aaacgattaa cacaaatacc acatttactt
    12541 tttactatga tgaagtcttt ctagttgaag tgaaagctcg tccaaataaa gtgtcggata
    12601 ctgatccgaa tatccaagaa ctctataatt atagtaagag ttatcctgtc gtttatggac
    12661 aaagcatcac aattgaagca cctacgttca ctaattttgt tcttgaccct agagaaagct
    12721 ctagcaacac tgtgacttta aacaacgtta caagcaatca aggaatcaat tttgcctata
    12781 ctcatcaata caacgtcact gtgaaccata taaacgttga cacaaacgct gttttatcta
    12841 ctgagacaca aacggtttac gagggagata atttctctac ttcttggaaa aatatgaccg
    12901 atcaaaacta cttcttgtgt agaaatgacg attcatctgt taccgttgat aaaaatggac
    12961 aacgtataat taatcatgtt gaccaaaatc gaacgatcac tttcaaatac aaaaatatct
    13021 cattaaatga tttaaacaac tatgtgcgtc aaaaagaact tgcttggtta aatagctacc
    13081 gtcaacaaaa tggcgttgct ccaatgcaat ttaacgacgt cgtacaaaaa gctgccgata
    13141 tacgtgcaaa agaattacaa gtaagcttca gtcattatcg acctggcggc ggtactttcc
    13201 aagatctctt agaaagttta ggctgctacg gcgctaaagg agaaaatatc aatagtttct
    13261 acctttacgt tgacgagatc ctttcagata cgggagcagt gaatgccatg attatgtgga
    13321 aagatagcgc tgctcataac gcaaacttac tttacaacaa tcaaagcatc gttggtttat
    13381 cccacgctta tgaagtttct gcagacggat cactttcttc agataatgta tttattagtg
    13441 ccaacccaat tttcaaatag aaaaacaagg ctgattttca gccttgtttt tttgcttaat
    13501 taaacttaag aaccacataa ctaaaagtag ctaacacaag taatagtaag ctgaccacta
    13561 aagttgttac taaatgtgta gaacctgttt taggaacaat aacttgtggc aagttaggat
    13621 caaccccaat tgtcacaaca tttgttttat tagaaacatt atctactgct actacggctt
    13681 ggttatcaat atttttttct gattgtagtg gggttttagc tgtaaatatc agttggattt
    13741 ctcgtccatc taaaagattg ttacttaaga agt
//
LOCUS       DAFEZB010000072        13380 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFEZB010000072
VERSION     DAFEZB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.072"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(45..1364)
                     /locus_tag="LP859_002235"
     CDS             complement(45..1364)
                     /locus_tag="LP859_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3030162.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LP859_002236"
     CDS             complement(1361..2014)
                     /locus_tag="LP859_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3030163.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LP859_002237"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LP859_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LP859_002238"
     CDS             complement(2724..3440)
                     /locus_tag="LP859_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL3030164.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="LP859_002239"
     CDS             complement(3490..3699)
                     /locus_tag="LP859_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030165.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3976..5249
                     /locus_tag="LP859_002240"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="LP859_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="LP859_002241"
     CDS             complement(5356..6387)
                     /locus_tag="LP859_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030166.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="LP859_002242"
     CDS             complement(6394..7224)
                     /locus_tag="LP859_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3030167.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="LP859_002243"
     CDS             complement(7221..8036)
                     /locus_tag="LP859_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3030168.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="LP859_002244"
     CDS             complement(8033..8857)
                     /locus_tag="LP859_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL3030169.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="LP859_002245"
     CDS             complement(8847..9947)
                     /locus_tag="LP859_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3030170.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="LP859_002246"
     CDS             10125..10910
                     /locus_tag="LP859_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL3030171.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="LP859_002247"
     CDS             10943..11326
                     /locus_tag="LP859_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL3030172.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="LP859_002248"
     CDS             11402..11533
                     /locus_tag="LP859_002248"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3030173.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="LP859_002249"
     CDS             11502..11738
                     /locus_tag="LP859_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3030174.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="LP859_002250"
     CDS             complement(11816..12787)
                     /locus_tag="LP859_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL3030175.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4066 a         2918 c         2313 g         4083 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
//
LOCUS       DAFEZB010000073        13145 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFEZB010000073
VERSION     DAFEZB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.073"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(77..149)
                     /locus_tag="LP859_002251"
     tRNA            complement(77..149)
                     /locus_tag="LP859_002251"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="LP859_002252"
     CDS             530..1099
                     /locus_tag="LP859_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL3030176.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="LP859_002253"
     CDS             1086..1946
                     /locus_tag="LP859_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3030177.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="LP859_002254"
     CDS             2136..2840
                     /locus_tag="LP859_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3030178.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="LP859_002255"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="LP859_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL3030179.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="LP859_002256"
     CDS             4677..5993
                     /locus_tag="LP859_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030180.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="LP859_002257"
     CDS             5994..6863
                     /locus_tag="LP859_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL3030181.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="LP859_002258"
     CDS             6918..7727
                     /locus_tag="LP859_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3030182.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="LP859_002259"
     CDS             complement(7830..8459)
                     /locus_tag="LP859_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL3030183.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="LP859_002260"
     CDS             8629..9921
                     /locus_tag="LP859_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3030184.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="LP859_002261"
     CDS             complement(9997..10659)
                     /locus_tag="LP859_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL3030185.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="LP859_002262"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="LP859_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL3030186.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="LP859_002263"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="LP859_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL3030187.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DAFEZB010000074        12756 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFEZB010000074
VERSION     DAFEZB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.074"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(11..160)
                     /locus_tag="LP859_002264"
     CDS             complement(11..160)
                     /locus_tag="LP859_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030188.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            556..756
                     /locus_tag="LP859_002265"
     CDS             556..756
                     /locus_tag="LP859_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3030189.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="LP859_002266"
     CDS             1324..1887
                     /locus_tag="LP859_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030190.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="LP859_002267"
     CDS             complement(1948..2292)
                     /locus_tag="LP859_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030191.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="LP859_002268"
     CDS             complement(2389..2634)
                     /locus_tag="LP859_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3030192.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="LP859_002269"
     CDS             complement(2704..2958)
                     /locus_tag="LP859_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030193.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="LP859_002270"
     CDS             complement(3122..3445)
                     /locus_tag="LP859_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030194.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="LP859_002271"
     tRNA            3632..3705
                     /locus_tag="LP859_002271"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="LP859_002272"
     CDS             complement(4090..4587)
                     /locus_tag="LP859_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3030195.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAECIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="LP859_002273"
     CDS             complement(4627..5436)
                     /locus_tag="LP859_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3030196.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="LP859_002274"
     CDS             5770..6627
                     /locus_tag="LP859_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL3030197.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="LP859_002275"
     CDS             6620..7522
                     /locus_tag="LP859_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL3030198.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="LP859_002276"
     CDS             7555..7905
                     /locus_tag="LP859_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL3030199.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7954..8877
                     /locus_tag="LP859_002277"
     CDS             7954..8877
                     /locus_tag="LP859_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3030200.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="LP859_002278"
     CDS             8955..9848
                     /locus_tag="LP859_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3030201.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="LP859_002279"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="LP859_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL3030202.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="LP859_002280"
     CDS             10463..10651
                     /locus_tag="LP859_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL3030203.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="LP859_002281"
     CDS             10664..11566
                     /locus_tag="LP859_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3030204.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="LP859_002282"
     CDS             11598..11840
                     /locus_tag="LP859_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3030205.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="LP859_002283"
     CDS             complement(12096..12467)
                     /locus_tag="LP859_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030206.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4516 a         2133 c         2396 g         3711 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acattctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatgtc
    12601 agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt tataatagat
    12661 ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac gaatggtaga
    12721 aatcaatttg cgatgcttac gattgatgat ctagtt
//
LOCUS       DAFEZB010000075        12246 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFEZB010000075
VERSION     DAFEZB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.075"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP859_002284"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP859_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL3030207.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP859_002285"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP859_002285"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL3030208.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LP859_002286"
     CDS             complement(2169..2906)
                     /locus_tag="LP859_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL3030209.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LP859_002287"
     CDS             complement(2997..3524)
                     /locus_tag="LP859_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030210.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LP859_002288"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LP859_002288"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL3030211.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LP859_002289"
     CDS             complement(4401..4886)
                     /locus_tag="LP859_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL3030212.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LP859_002290"
     CDS             complement(5039..5236)
                     /locus_tag="LP859_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030213.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP859_002291"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP859_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL3030214.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP859_002292"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP859_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL3030215.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LP859_002293"
     CDS             9211..9423
                     /locus_tag="LP859_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL3030216.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LP859_002294"
     CDS             9425..11104
                     /locus_tag="LP859_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL3030217.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LP859_002295"
     CDS             complement(11358..11558)
                     /locus_tag="LP859_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3030218.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="LP859_002296"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="LP859_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3575 a         2636 c         2117 g         3918 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaat
//
LOCUS       DAFEZB010000076        12176 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFEZB010000076
VERSION     DAFEZB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.076"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(138..302)
                     /locus_tag="LP859_002297"
     CDS             complement(138..302)
                     /locus_tag="LP859_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030219.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            597..1925
                     /locus_tag="LP859_002298"
     CDS             597..1925
                     /locus_tag="LP859_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL3030220.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="LP859_002299"
     CDS             2127..2960
                     /locus_tag="LP859_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL3030221.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="LP859_002300"
     CDS             2976..3713
                     /locus_tag="LP859_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL3030222.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4836)
                     /locus_tag="LP859_002301"
     CDS             complement(3868..4836)
                     /locus_tag="LP859_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL3030223.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5068..5901
                     /locus_tag="LP859_002302"
     CDS             5068..5901
                     /locus_tag="LP859_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL3030224.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5935..6774
                     /locus_tag="LP859_002303"
     CDS             5935..6774
                     /locus_tag="LP859_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3030225.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6800..7360
                     /locus_tag="LP859_002304"
     CDS             6800..7360
                     /locus_tag="LP859_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL3030226.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7559..9280
                     /locus_tag="LP859_002305"
     CDS             7559..9280
                     /locus_tag="LP859_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL3030227.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9445..10587
                     /locus_tag="LP859_002306"
     CDS             9445..10587
                     /locus_tag="LP859_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL3030228.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10603..11814
                     /gene="thiI"
                     /locus_tag="LP859_002307"
     CDS             10603..11814
                     /gene="thiI"
                     /locus_tag="LP859_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL3030229.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3456 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4861 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4921 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     4981 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5041 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5101 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5161 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5221 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5281 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5341 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5401 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5461 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5521 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5581 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5641 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5701 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5761 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5821 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5881 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     5941 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6001 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6061 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6121 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6181 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6241 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6301 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6361 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6421 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6481 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6541 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6601 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6661 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6721 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6781 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6841 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6901 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     6961 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7021 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7081 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7141 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7201 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7261 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7321 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7381 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7441 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7501 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7561 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7621 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7681 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7741 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7801 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7861 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7921 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     7981 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8041 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8101 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8161 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8221 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8281 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8341 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8401 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8461 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8521 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8581 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8641 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8701 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8761 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8821 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8881 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     8941 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9001 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9061 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9121 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9181 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9241 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9301 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9361 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9421 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9481 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9541 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9601 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9661 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9721 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9781 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9841 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9901 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
     9961 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10021 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10081 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10141 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10201 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10261 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10321 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10381 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10441 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10501 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10561 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10621 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10681 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10741 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10801 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10861 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10921 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    10981 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11041 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11101 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11161 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11221 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11281 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11341 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11401 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11461 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11521 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11581 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11641 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11701 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11761 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11821 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11881 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    11941 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12001 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12061 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    12121 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttg
//
LOCUS       DAFEZB010000077        11923 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFEZB010000077
VERSION     DAFEZB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.077"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            255..530
                     /locus_tag="LP859_002308"
     CDS             255..530
                     /locus_tag="LP859_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030230.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="LP859_002309"
     CDS             543..2621
                     /locus_tag="LP859_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL3030231.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="LP859_002310"
     CDS             2781..4667
                     /locus_tag="LP859_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3030232.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="LP859_002311"
     CDS             4679..5626
                     /locus_tag="LP859_002311"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL3030233.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="LP859_002312"
     CDS             5646..6173
                     /locus_tag="LP859_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3030234.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="LP859_002313"
     CDS             complement(6236..6889)
                     /locus_tag="LP859_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL3030235.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="LP859_002314"
     CDS             7021..7863
                     /locus_tag="LP859_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3030236.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="LP859_002315"
     CDS             8021..8917
                     /locus_tag="LP859_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL3030237.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="LP859_002316"
     CDS             8920..9633
                     /locus_tag="LP859_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL3030238.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="LP859_002317"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="LP859_002317"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL3030239.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="LP859_002318"
     CDS             10168..10395
                     /locus_tag="LP859_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL3030240.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="LP859_002319"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="LP859_002319"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL3030241.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="LP859_002320"
     CDS             complement(11181..11681)
                     /locus_tag="LP859_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL3030242.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT         4164 a         2022 c         2430 g         3307 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgt
//
LOCUS       DAFEZB010000078        11735 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFEZB010000078
VERSION     DAFEZB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.078"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(94..969)
                     /locus_tag="LP859_002321"
     CDS             complement(94..969)
                     /locus_tag="LP859_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL3030243.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="LP859_002322"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="LP859_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL3030244.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="LP859_002323"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="LP859_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL3030245.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="LP859_002324"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="LP859_002324"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL3030246.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="LP859_002325"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="LP859_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL3030247.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="LP859_002326"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="LP859_002326"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL3030248.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="LP859_002327"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="LP859_002327"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL3030249.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="LP859_002328"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="LP859_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL3030250.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="LP859_002329"
     CDS             complement(9420..10187)
                     /locus_tag="LP859_002329"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL3030251.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="LP859_002330"
     CDS             complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="LP859_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL3030252.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11300..11698
                     /locus_tag="LP859_002331"
     CDS             11300..11698
                     /locus_tag="LP859_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3030253.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         3097 a         2553 c         2057 g         4028 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    10681 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    10741 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    10801 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    10861 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    10921 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    10981 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    11041 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    11101 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    11161 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    11221 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    11281 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    11341 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    11401 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    11461 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    11521 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    11581 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    11641 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    11701 agataactaa tctgacgttt tttataacaa aaaaa
//
LOCUS       DAFEZB010000079        11297 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFEZB010000079
VERSION     DAFEZB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.079"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            453..1025
                     /locus_tag="LP859_002332"
     CDS             453..1025
                     /locus_tag="LP859_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL3030254.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1170..1874
                     /locus_tag="LP859_002333"
     CDS             1170..1874
                     /locus_tag="LP859_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3030255.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            2102..2425
                     /locus_tag="LP859_002334"
     CDS             2102..2425
                     /locus_tag="LP859_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030256.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQI"
     gene            2425..3279
                     /locus_tag="LP859_002335"
     CDS             2425..3279
                     /locus_tag="LP859_002335"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL3030257.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="LP859_002336"
     CDS             3426..3659
                     /locus_tag="LP859_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL3030258.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3857..5761
                     /locus_tag="LP859_002337"
     CDS             3857..5761
                     /locus_tag="LP859_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL3030259.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVDVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAVPVNKQADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVGTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEETEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            6071..6433
                     /locus_tag="LP859_002338"
     CDS             6071..6433
                     /locus_tag="LP859_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3030260.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILNASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIHVFLKLSENLSMKS"
     gene            6450..7058
                     /locus_tag="LP859_002339"
     CDS             6450..7058
                     /locus_tag="LP859_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HBL3030261.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGLRQIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVALVGEEEEEEEVVEKLES
                     RGTNRFFWTVALITIASGGDNLGIYIPYFASLSFSEIVTALIVFAISVAVLCYISYKL
                     AKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(7160..8068)
                     /locus_tag="LP859_002340"
     CDS             complement(7160..8068)
                     /locus_tag="LP859_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL3030262.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIVTVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(8110..8424)
                     /locus_tag="LP859_002341"
     CDS             complement(8110..8424)
                     /locus_tag="LP859_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL3030263.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8723..10654
                     /locus_tag="LP859_002342"
     CDS             8723..10654
                     /locus_tag="LP859_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL3030264.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSINGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTDTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10948..>11297
                     /locus_tag="LP859_002343"
     CDS             10948..>11297
                     /locus_tag="LP859_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3030265.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKV"
BASE COUNT         3665 a         1872 c         2367 g         3393 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tataatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtaaaa tcagttaacg gtgttgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa gccaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg tccttaaagc aaaagtcaag gccgcttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacattct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 cgtggatgat atagatgaag gggatatctt actcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggggaagc
     4381 tgttccggtc aataagcaag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggta aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcagaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagcaatgt gggtacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg ggaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa gaaactgaag tcgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc ttagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggccacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcaattg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcga aaagaacaaa aagaagtatc agcacctgca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaatgatt aaaaaattta ataataaaat
     5941 ttaaattcct attaagttgt aacacatgaa atttcttata atagtgtatt gaaattaagg
     6001 tagattgaca aacgagtgat caaatagata tactaccttc aaacgaccgt ttgaatgtga
     6061 ggtgagatga atggaaaaag tttgccaagt gactatcgta cataaagaaa aagtaaatgt
     6121 aataaaaaaa aaattggatc aaaaagattt ttcaaactta cttgtgttag ggaaatgttt
     6181 cagcgattct tctagaatta aaatttttta tgctttagaa acctataagg aaatgtgtgt
     6241 ctgtgattta gcagaaatac ttaatgctag tgtcgctaca acgtcacacc atttacgttt
     6301 tttaaaaaaa catggaatgg caaaatcacg tcaagatgga aaagtagttt attactcttt
     6361 agcgaatgaa gacatccttt ccgctattca tgttttttta aagttatcag aaaatctatc
     6421 tatgaaatct tagtttggag gaagttattt tgttacaaag tattttttca gctatcgctg
     6481 tttatatttc taccagcatt gattatttgt ttatcttgct gattattttt tctcaaagtc
     6541 atacaaaaaa agggctccgt cagatttttt ggggtcaata tctaggaaca ggtatcttag
     6601 tagccgtaag tttatttgca gcatatgtgc tgaatttcat tccgcaagat tggatcattg
     6661 ggcttctcgg tctaattccg attttcttag gaatcagagt ggcattggtt ggcgaagagg
     6721 aggaagaaga ggaagtcgtt gaaaagcttg aatcaagagg aacaaaccgc ttcttttgga
     6781 ccgttgcctt aataacaatt gcttctggtg gcgataactt agggatttat attccttact
     6841 ttgcctcgtt atctttttca gagattgtca ccgctctaat cgtatttgct atttctgttg
     6901 cggttctttg ctatatcagt tacaaattag ccaagatttc ttttgtttcc gaaacgttag
     6961 aaaaatacga acgaatcatt gtccctataa tgtttattgc gttaggaatt tttattatga
     7021 tagagaacgg gacgatacaa acgctactag gtttttaaaa atcataatca cgaaagataa
     7081 aaagagaggc agcgattttg attagtcgct gccttttttg cttttcaaat aatgaagtat
     7141 tttttagcgt agaactatct tacgtgttgt gggagtatcc cttttcaagc tcttctttgc
     7201 ttgtagttat ttctattgtg atatgattct ctacgcttga tttgtgtgcg acttctgcaa
     7261 tttcttcttt tatttcccta gccttgtcta tgtctgaagg gtctataaga accgtgacga
     7321 taagggcgtt ttcttccccg tccagtgacc aaatgtgaaa atgtgagagg tcttttacag
     7381 caggaacatt tttaatggag tccgccaagc tctttacgtt tacattttca ggcgaaccat
     7441 tcaataaaat tttcattgtg gtgaaaaatt caggaacggt tttgtagagg atataaagtg
     7501 caatcaaaat cgataagata ggatccagac gatatgcctc ggtaaagttc attagaatac
     7561 tcacaatcaa gactccaatc caccctaata cgtcttccag catatggagg ttcaacatgc
     7621 tctcgttttt ggatgtgcct ttgcttaaaa tccacgcagc atatccattc aatgcaatag
     7681 ctacgagaga aagccagaac atgccactat aatttacggg ttgtgggtca atcaaacgtg
     7741 gcacactacg atagatcata aaaaacgagc cactaattag cacaacgcca gttatcagcg
     7801 cgcccagtaa agaaaagcgg ttatagccaa aagtgaaccg ttcatcttgt tttttctctg
     7861 aataaccttg aaaaaaccaa gcgaatccaa ttgaaataga gtcccctaaa tcatgaactg
     7921 cgtcggacat aatggacaca ctattgaaaa gaaagccaaa gaaaaattcg cttatagaga
     7981 aaataagatt tatgaagaaa gcaattttaa tattcttttt cggttgattc tgattcgagt
     8041 gattatggtc tttatctgat tttgacattt aaaaacctcc taagtttgta tgctacatac
     8101 taattggttt tatttttcta attcattaac atgagttaag acttgttcga gaatactaaa
     8161 aacatgaagg tcatctaaac tgtaaagcat actttttcct tctcttcttg atttcactaa
     8221 gcgtgcgtct ttcaacgttt ttaattgatg agaaatagcg gactgttcca tgtccagaga
     8281 aatagcgata tttccaacac tacactcttt tttctgtaaa agaaagagaa ccgacaatcg
     8341 tgtaggatca ctaatcacct taaatagttt gcttattgac agaatagatc ctttatcgat
     8401 tggagaagtt gtttttatat ccatagtgtt tttattcctt tcatatgacc tttcgttcat
     8461 gtctatatta ccatatgagc gaaacaatgc taatcttttg tttacaaaag agaagtggcg
     8521 ttctctttat cttgacaaaa tgtatatagc gatataaaat gagtatacaa acatatgatt
     8581 aattattcat atgtaaagta gattcattac gatttacttt tggaataaac gaaaggagtc
     8641 agaaatagca gaaatttatg tgattcaaga agtaaatgat ggaaaacaaa ggaattataa
     8701 attatatagg gaggcaatag aaatggaaac tatccaagaa caacagtctc aagaaaagca
     8761 taatcataat cacaaccacg atcacgacca tgatcatggg aaaatgccag tcgttttgta
     8821 ctttgttggt ttagcattag cagtgattgc tctatttttg aatgaagatt atcagttggt
     8881 acaaaatgtt ttgttctcaa tcgctacaat cagtgccggg tatcacgtta ttcttcttga
     8941 aggccttgga gaaacaattg agaattcaaa agcacaaaaa aagttcatac ctaattctca
     9001 tattttaatg ggattagcgg caattggagc ttctttgatg ggtaattttt gggaaggaac
     9061 attattgatt cttatttttt ctggcgcaca ttttttggaa gattatgcag aaggaagaag
     9121 caaacgagaa ataacgaaat tactcgaaat gaatccaacg actgcccggt taatcctgcc
     9181 tgatggtagt acaaaaaatg tagatgtcag tgaattaaaa gtgggagatc aacttcaagt
     9241 gttgaatggc gatcaagtac ctattgatgg cgtcattttg tctggatcta catccattga
     9301 tgagtcgtct attaatggag aaagtatccc gaaagagaaa tccaaaggag atggcgtctt
     9361 cgggagtaca atcaatggaa caggtacctt cacgatgaaa gtcaccaaag aaaacaaaga
     9421 tacagtattt tcaaaaattt tacaattagt gaatcagaac caagataacc aaacaaaagc
     9481 tgcgagtatt atccaaaaat ttgaaccgaa atatgtcaca gttgttttaa ttgccattcc
     9541 tctattcatg ttattggctc cttttctact tgattggaca tggtcacaaa gtgtctatag
     9601 aggattggtc cttttagttg cagcttcacc gtgtgcttta gcagcagcta ctgtctcggc
     9661 aacgctatcg acgacttcta atttagccaa aaaaggagtc ctttcaaaag gtagttctta
     9721 cctgtctcaa ttagccgata ttaaagcaat tgcatttgat aagacggaca ccttgaccaa
     9781 aggaaaacct gaagtaacca attattattt tgctgattcc gtgaacgaag aaaacatgat
     9841 tgatatcgta gtagctcttg aaaaagaatc caatcacccc ttagcagatg ctattctaag
     9901 aaagtttgag caaaaaaaca aactaactat tgaagtagaa aaccaaattg gtaaaggatt
     9961 gacgggagat tacaaaggga gaaattatcg tataggaaaa ccgacttcgt ttgatgatgt
    10021 ttcagatgaa tatagtcgtt taaataatga atgggcgtca gaaggtaaga cagtcgtata
    10081 cgtagcagta gatgaagagg ttataggcct tattgccctt atggacgttc cgagtgaata
    10141 tgctaaagaa accattgaat attttaggaa acaaggtata cacaccacat taattactgg
    10201 tgactcagaa atgacgggaa aagcggttgc taaacaatta ggaatagatg aagtgattgc
    10261 gaatgtcatg ccggaagaca aatccagaat tatagacgaa caaaaagaaa aatacggcgt
    10321 gaccgccatg gttggagacg gtgtaaatga tgctcctgct cttgttaatg cagatgtggg
    10381 aatcgctatg ggagatggga ctgatgtggc agtagaggta tctgatttag ttttaatgca
    10441 aaacaattta tctaaattgg tacaatctca taatatttct tcgaaaatga accgtgttat
    10501 ttggcagaat attatttttt caatggccgt tgttgctttt ttaattgtcg ttagtttctt
    10561 aggcttaacg gatattgcaa tcagtgtcat tattcatgaa ggaagtactt tggttgttat
    10621 tttgaatgga cttcgattgc taaaggcagt ctaaagaatg attgcacaac ttcaaattaa
    10681 tcgagtaata gagataatta aggattacac tacatttttt tatctctatt acttgtttat
    10741 atttatctag atgttcaaag tcaaaatgat atatcaacca tactcttgtg gcgcgggttg
    10801 aatataagat acatgaaatt gtataaaatc atgtatcaag aatcctatcc ttatagggtt
    10861 cttttttttt actcccccta aacacacgat ttcgtgttgt aataactata atcatgtatt
    10921 cgaacagact aaaaaggagg gattgtcatg ggctataatg ttcagccact acggtcgcaa
    10981 caggagatta atgatttttt attttgcctg cgacggaata aaaatgcgga acgtgacgtt
    11041 ttcttgtttc taattggaat taacagcggt ctgcgcatgt ccgacatcgt taaactcaag
    11101 aaaaaagacg tgattacctc aaaaaacccg cgcatcgtgg agcaaaagac agggaaaaca
    11161 cgcattttat acttaagcag cttacaggac ttgattcacg gctataccgc acccttagat
    11221 ccggaggctt acttgtttcc cagtactaag ggcggccatt tagaagtcaa tacggtctac
    11281 cagatgtttc aaaaggt
//
LOCUS       DAFEZB010000080        11236 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFEZB010000080
VERSION     DAFEZB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.080"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            82..1128
                     /gene="gmd"
                     /locus_tag="LP859_002344"
     CDS             82..1128
                     /gene="gmd"
                     /locus_tag="LP859_002344"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBL3030266.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1208..2221
                     /locus_tag="LP859_002345"
     CDS             1208..2221
                     /locus_tag="LP859_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030267.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2258..3202
                     /locus_tag="LP859_002346"
     CDS             2258..3202
                     /locus_tag="LP859_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBL3030268.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3270..4583
                     /locus_tag="LP859_002347"
     CDS             3270..4583
                     /locus_tag="LP859_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBL3030269.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4667..5569
                     /locus_tag="LP859_002348"
     CDS             4667..5569
                     /locus_tag="LP859_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBL3030270.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5574..7226
                     /locus_tag="LP859_002349"
     CDS             5574..7226
                     /locus_tag="LP859_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL3030271.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7250..8116
                     /locus_tag="LP859_002350"
     CDS             7250..8116
                     /locus_tag="LP859_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030272.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8158..8349)
                     /locus_tag="LP859_002351"
     CDS             complement(8158..8349)
                     /locus_tag="LP859_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030273.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8379..8918
                     /locus_tag="LP859_002352"
     CDS             8379..8918
                     /locus_tag="LP859_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030274.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9046..9399
                     /locus_tag="LP859_002353"
     CDS             9046..9399
                     /locus_tag="LP859_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030275.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9866..10342
                     /locus_tag="LP859_002354"
                     /pseudo
     CDS             9866..10342
                     /locus_tag="LP859_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10342..10536
                     /locus_tag="LP859_002355"
     CDS             10342..10536
                     /locus_tag="LP859_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030276.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10542..10827
                     /locus_tag="LP859_002356"
                     /pseudo
     CDS             10542..10827
                     /locus_tag="LP859_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10841..11207
                     /locus_tag="LP859_002357"
                     /pseudo
     CDS             10841..11207
                     /locus_tag="LP859_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3153 a         2303 c         2854 g         2926 t
ORIGIN      
        1 tatcgtgtat tcttgaaaag tattagaaat tacgagccat ttatatggtc ttatcactta
       61 cataggaaag gataatgtat catgaaaaaa gcgctgatta ctggtattac tggtcaggac
      121 ggttcctatc tggcagattt cctgctagag aagggctacg aagttcacgg tatcactcgc
      181 cgtgcttcta tttccaatac cgctcgcatt gaccatttga tgggcaagat cacccttcat
      241 gacggtgacc tgtccgactc atcttctctg attcgtatca tcagcattgt tcagcctgat
      301 gaaatctata acctcgctgc acagagccat gttcaggttt cttttgatgt tcctgagtac
      361 tccggcgatg ttgatgcttt gggcgttatg cgtattctgg aagcttgccg tattctgggc
      421 ctgacgaaga agactaagat ttatcaggca tctacttctg agctgtatgg caaggttgaa
      481 gaagttcctc agcgtgaaac cactccgttt catccttaca gcccttatgc tgttgcaaag
      541 cagtatggct tctggatcac taaggaatat cgtgaagctt atggcatgtt cgctgtcaac
      601 ggtattctgt tcaaccatga atccgaacgt cgcggtgaga atttcgtcac ccgtaagatc
      661 accctcgctg ccggccgcat tgcagaaggc ctgcaggacc atctggaact gggcaacatg
      721 gactccctgc gtgactgggg ctatgcaaag gactatgttg agtgcatgtg gatgattctc
      781 cagcatgaga ctcccgagga cttcgtgatc gctaccggcg agcagcacac tgttcgtgac
      841 ttcaccgaga aggcattcgc agcaaatggc atgaccattc gttgggaagg cactggcatt
      901 gatgaaaagg gctacgatgt tgaaaccggc aagatgctcg tttgtgtaaa tccacagtgg
      961 ttccgtccga ctgacgttga caacctgtgg ggcgacccga ctaaggcaaa gaccgttctc
     1021 ggttggaatc cgcagaagac ctcttacgag gaactggtta acatcatggc aaagcatgac
     1081 cgtgaattgg caaagcgcga agctgcaatg aagaacgcag tcaagtaagc tgacattgaa
     1141 taaacatatc tgtttctatc tttccagtca cggcttcgga catatcgcgc ggaatctgcc
     1201 cattattatg gcaacactct gccgatgcga agcgaagatt acactggttt gcggcgcaaa
     1261 tcatctggat tttgcaaagc gcaatttgga gcaggaactg cccaaagagc tgatagaccg
     1321 cgtgcgctat cgtccaataa acacagatat cggtctgatt aaccgtgagg gcaccctact
     1381 ggtggacacg ggtaagctga ccgagatctg tgaatatttt ttggcaaagg ttccttcttt
     1441 ggcaaagaca gaagccgaat ggctcaagcg agaagctgtc agcgcagtgc tttgcgatat
     1501 gccaatctgg gcaatggatg cctgcgatat ggcaggagta cctttcctgt atgtaggaaa
     1561 cttcacttgg acagagctgt atcgcgaatt tttacccgaa catatttgga aagaatatgc
     1621 caagcgttac ggtaaaattc agcacaccat gctttatgcg ctaaataacg aagaaatgtt
     1681 ggaatttctc caaaacagcg aaaaaacacc gacttccgtt gtggcgcgtc cgattcataa
     1741 cgacgaagtg caacgcatta aatcacagca tgaaaaaccg cctgtatttg tcgcactcgg
     1801 tatgtccgcg cggttcaaac agccaattga tgtcagtcac gcgccgtatg atttcattac
     1861 aacgctaggt gttccgcttg tgggagaaaa cgtaaccgtt atttctaaca gcacccccaa
     1921 tacacaggac tatatagcgg cggcagatta cgttattaca aaggctggat ggggaactgt
     1981 tgcggagtgt attctcgcac agcgtccgat ggcactgttc gcccgcgata cagttttgga
     2041 agatagaaca actatttgta aattgcaaaa actgggtctg gcaatttccg tcacaccgga
     2101 aaacctccac cgcgtagagc gggtgattga ggacctcaaa cagctagaca cctcaaattt
     2161 tgatacattt tatgatgctg cgagcgaaat cgcagacaaa ttgatttctc tcatgcaatg
     2221 agagatgctc taaatttaga aaggagcaag cattattatg atggaaaaag acgcaaaaat
     2281 ctacgtagcc ggtcatcgtg gcatggctgg ttccgcaatc gtgcgcgagc tgaaccgtca
     2341 ggactacaac aatattatca cccgcaccca taaggagctt gacctgtgcc gtcaggacgc
     2401 tgttgaagca tttttcgcac aggaaaagcc ggattatgta tttctcgctg ctgcaaaggt
     2461 tggcggtatt atcgcaaacc agaacgctct ggcagacttc atgtatgaaa acatgattct
     2521 ggaaatgaac gtcatcaact ccgcatggaa gaatggctgt aagaagttac agttcctcgg
     2581 ttcttcctgt atctatccgc gtatggcacc gcagccgatg ccggaaagct gtctgctgac
     2641 aagtgagctg gaaaagacca acgaggctta tgcactggca aagatttctg gcctgaagta
     2701 ctgtgagttt ctaaacaagc agtacggtac tgactatatc tctgttatgc ctactaacct
     2761 gtatggacct aacgacaact atcatcctac tcacagtcat gtgctccccg ctttgattcg
     2821 tcgcttccat gaggctaagg aagcaggtct gcctactgtt acctgctggg gtgatggttc
     2881 tcctctgcgc gaattcctat atgttgacga tctggcaaac ctgtgtgtgt tcctgatgaa
     2941 taactatagc ggtgacgaga ctgttaatgc aggtactggc aaggaactgt ctatcaagga
     3001 actgaccgag atggtagcaa aggttattgg ttacgaaggc gagattctgt gggataccag
     3061 caagccgaac ggtacacctc gtaagctgct ggatgtttcc aaggcaacca agctgggatg
     3121 gacatataag actgagttgg aagatggcat ccgtctgtcc tacgaggact tcctgaataa
     3181 cccgatgcgt gctgaacgct aatttaccga ggttgctgtg atttttgagg attgcagcag
     3241 cctctttctt ataaaaagga gagatcatta tgaatattgt tttactgtct ggcggttctg
     3301 gtaagcgcct ttggccgctg tctaatgaca tccgttccaa gcagttcatt aagattttca
     3361 aaactgaaga tggatatgaa tccatggttc agcgtgtcta ccgccagatt aagaaggtag
     3421 ataacgacgc aactgttacg attgcaacct ctaagacaca ggtttctgct atccataacc
     3481 aacttggtga ggatgtgggt gtatctgtag agccttgtcg ccgcgatacc ttcccggcaa
     3541 tcgcattggc taccgcttat ctggtagatg tgatgggcat tgatccagaa gagtccgtag
     3601 ttgtttgccc ggttgaccct tacgtagaag atgactattt tgaggccctg aaatctctgt
     3661 ccgatcaggc tgacaaaggc gaagctaatc tggtgctcat gggtattgaa ccgacttatc
     3721 cttccgagaa gtacggctac atcattcctg agagcgcaga gcagactgct ttcgtaaata
     3781 ccttcaagga aaaaccttct gaggctgtgg caaaggaata tatcgcacag ggtgcactgt
     3841 ggaacggtgg tgtgtttgtc tacaagctga agtatgtcat ggagcgtgca catcagctga
     3901 ttgactttgt agattatcag gatctgttca acaaatacga tactctgact aagatttcct
     3961 tcgactatgc cgttgttgag catgaggaca agattcaggt acagcgtttt gctggtatgt
     4021 ggaaggatct ggggacttgg aatactctga ccgaaggtat ggaggaatcc attatcggta
     4081 aaggtgttat gaatgagaat tgcgatggcg tacacatcct gaacgaactg gatgttcctg
     4141 tgttggctat gggtctgcat gatgtggtca tctctgcatc tcgggaaggt attctggttt
     4201 ccgataagga acagtccagc tatattaagc ctttcgttga caagttcgag cagcagatca
     4261 tgttcgcaga gaagtcttgg ggtagttata aggttatgga tgtagaagca gagagcatga
     4321 ccattaaggt tactctgaat gctagtcact ccatgaatta tcacagccac aagaaccgtg
     4381 acgaggtgtg ggtggttctg gccggtactg gtaagaccat cgtggatgga tatgagcagg
     4441 ttgttaaggc tggcgatgtg atcaccatga gtgctggctg tcggcatacc attatggcag
     4501 atactgagct gaagctgatt gaagtacagc ttggcaagga tatcaatgtt aaagataaac
     4561 agaaatttga gttagagtat taaaaaacgg aggagggctc gttatacaga aaccgttttt
     4621 attgaagcct tccgcaaagg attatctgtg ggggggaacc gcctgaatga tgaatttgca
     4681 aaaggaattg agatgtctcc tcttgcggaa acttggaatg ctctacgcac ccggatggac
     4741 cgagctttgt tgtgtctggt cagagtatca ggggatgacc ttgacggagg tgttgcaaga
     4801 acacccagag tttctgggaa cgcatccgca gaccaaagga gaattgccga tcctgatcaa
     4861 attcattgat gcgaaaaagg atttgtctgt tcaggtacat cccacagatg aatatgcaat
     4921 tgaaaaagaa aacgggcagc ttggaaagac agagatgtgg tatgtactgg atgccagctt
     4981 aatctatggg ctgcagaaaa taacagaaga agctgtgatc cgtgaagcga ttgctcaggg
     5041 aactgttgag cgttatcttc agaaggttcc ggtcaaaaag aatgatgtgt tctttattga
     5101 ggcgggaacg attcatgcga ttggtgcggg tgcactaatt gcagaaattc aggaaaattc
     5161 caatctgact tatcgtctct acgattatga ccgtgttgac aaaaatggta aaaagagaga
     5221 acttcatgta gacaaagcgc tagaagtagc gaacttgaaa gggttgtcag aaccgaaaca
     5281 gccgcttcgt gttctaaaat atcgtgctgg tgtagctgcg gaattgcttt gcagatgcaa
     5341 gcatttcgag gtctctcgta tgattgtcaa cactgagaga agacagacgg tacactatcg
     5401 tgcggattca gtcagcttcc gtgtgttgct gtgtgtcaat ggctgtggta ccgttcgatt
     5461 tgataatgaa tcgttggaca tctataaggg ggactgcctt tttgtgccgg ccgattctac
     5521 tgaaatcagc attcacgggt cgattcagtt tctggatgtg agaggataat aatatgaatt
     5581 ataaagtaga atatgagcgt tggctgaatc atgccaatgc agatattata gaagaactgc
     5641 gttcctattc tgagaaggac attgaggact ctttctataa aaatttgtct ttcggtactg
     5701 gtggtctgcg tggtaccatc ggtgcaggca ctaatcgcat gaacgtacac accgtaggta
     5761 aggcaagcca aggtctggca gactatctga ataagaacta tgaagccgct tccgtggtgg
     5821 ttggctatga ttcccgtatc aaatctgatg tgtttgcaaa gactgccgca gacatctttg
     5881 ctgcaaatgg tattcaggtg cacttgtggc cagaactgct tcctgttcct acagtatctt
     5941 ttgcgactcg ttatctgaag gcagatgccg gtgttatgat tacggcatct cataaccctt
     6001 ctaaatataa cggctacaag gtatacggtg cagacggctg tcagattact accgaggctg
     6061 ctactgcaat tctggcagaa atcgaaaagc tggacatctt tgctgatgta aagatggatg
     6121 gcgatgcttc taagattgca tccattcagt cggaagttct gactgctttc atcgaagaag
     6181 tgaaggggca gtccgtcctg tttggcgaaa aagtggacaa gaatgttgct atcgtgtatt
     6241 ctcctctgaa tggtaccggt ctggtgcctg ttacccgcac cctggcagag atgggataca
     6301 gcaatgtgac cgttgtggaa gaacagcgtc tgccggacgg taatttcccg acttgtcctt
     6361 atccgaaccc tgaaattaag gaagctatgt ctctgggtat ggagtatgcc aagaagtaca
     6421 atgcggatct gctgttggcc actgaccctg actgcgaccg tgttggtatc gcagttaaga
     6481 gcgcacatgg tgactatgtt ctgttgtctg gtaatgagac tggtctgcta cttctggact
     6541 acatctgttc tcagagaatt aagcatggaa aaatgcctgc tgaccctgtg atggtcaaga
     6601 ccatcgtcac catggatttg ggtgaacgta tcgcatccaa ctatggtgtt cgtaccatcg
     6661 atgtgctgac cggctttaag tttatcggtg agcagattgg tatgttggaa aaggctggta
     6721 aggctgacag ctatatcttc ggctttgagg aatcttacgg ctatctgtcc ggtagctatg
     6781 tccgtgacaa ggacggtgtt aacgctgctt atctaatctg tgaaatgttc tcttactaca
     6841 agactcaggg tatcagcctg ctggataagc tgaatgagat ttatacaaag tatggctatt
     6901 gtttgaacac cctgcattcc ttcgagtttg acggtgcggc aggttttacc aagatgcagg
     6961 aaattatggt tgaattccac aagggtctcg atataattgg tggtaagaag gttattgaaa
     7021 ctttagacta cagtaagggc ctgaatggtc tgccgaagtc tgatgttttg aagtttatgc
     7081 tggaagataa ctgctctgtt gtggttcgtc cttctggtac tgagccgaag ctgaagacct
     7141 atatcagcgt cagcgcagag aacaaggaag ccgccgaagc tatggaagct attatggctg
     7201 ctgaactggc ggaaatgttc gagtaagaaa agaaaggaga aatcttgtaa tggaggaact
     7261 gttttctaac gctttgaaat cggtgaagtc aaatcgtgtg ctgtacacgg catcgttctt
     7321 tgcaagatcc tctcttttgt atttgcagga aattggacag ctgactgctc tgaaggcaca
     7381 tacatctagg cgtacaggat tgtcatctta cctttgtttc ctggtagaag ttggttcagg
     7441 cacattggag tataaggggc agaaatatac gcttcaagta ggagatatgg tgtttattga
     7501 ctgtttgaag ccgtatgtcc atgagtcatc agaagatgat ttgtggactt tgcggtggtg
     7561 ccattttaat ggcattggta tgacggaagt ctacaaaaaa taccttgaac gaggtggtca
     7621 accggtcttt aaaacatcaa aaacagataa gtatgctgac ttgctgagga gccttcatat
     7681 ggtggcagag ggacaggagt atgtccggga tatgaaaatc aatgttctac tcagtgaact
     7741 gctgatgcat ttgatggaag attcttggaa tcctgacaaa gtgccgaatt tctctacaaa
     7801 aaaaatggaa ttgcagaaca taaaacaata tctggatgac cattacacgg agaagattac
     7861 gctggatcag ttgagtgcag agttttttat caataaacag tacttagcta agatttttcg
     7921 tgaaacttac ggtacaactg ttgtcggtta tctgaatcag aaacgaatca cacaggccaa
     7981 gcacttgcta cgctttacaa atctaagtat ggaagagatt gcaacaaaaa ttggcataac
     8041 agatgcaaac tatttttcca gaactttttt caaggtggaa ggtgttcgtc ccagcgaatt
     8101 ccgcaagatg tggtaataaa aagccggcgg tgttgagccg ccggtcagta cctgtcgtta
     8161 acccgcattc aaagctttaa tctcagcttt gatttcagca cgaacacgat agtaatcttc
     8221 agtttcgatt tcggtatcca gccgagttgc tgtctttccg cagtcaggac aacgctctgg
     8281 ctgttcgtcg gcagggaagc tgtagtggca ggaatcacag gtgtaaatgt gatcgtcacg
     8341 tataatcatc acctaccaat ccagtgctta gatttttcat gcaattacta ttatatcgaa
     8401 ttggtgattc cacaaaaatc cgcttcttga taaatgtacg attcagagtg atatatagta
     8461 ctgtactgcc aaagggaata ccacaacggc agaaatggat gattcacatg gaagaaatta
     8521 tgcagatgat tttagggaac gcaaaattcc agctcggaca ggtggtttcg acccctgctg
     8581 tattggaaac tttcaacccc ctgcagaccg caaacggcat tcccgaagcc ttgagcaacc
     8641 tttggtttag acaggaaatt agtattgcgg agatcgagga cgcagttctg gatgccaaga
     8701 acggcaagga gctagcaaac agcctcaacc gcatcaagct tttccgcaag ttcaccctcg
     8761 accgagagct ggacggcatg gtcagactga agggcgcgga cgtctggaag aacatcagct
     8821 acctcaaggt ctacaccgcc acgccgccta agaaaccgac ccccccaagg ttccagtcga
     8881 acggctggag ccttttcttc tctcataaat cggcataatg tatacaaata cagctccaaa
     8941 tgattgtgta gtatatgcct gtggattgac ttgatatagt cgagagacta tggcaatata
     9001 cacacaacca aaggggaaaa tcccaaaaac aaggaggaaa cgctaatggc aatgagcgaa
     9061 aaggcaaaga aatatttgaa ggagatcaag ggtgcaaaga ccatcccgga gctgaaggat
     9121 gtggagatcg caatcaagcg agacggtatc cttgcttggg cagagttcac caagttgaac
     9181 gaggcggtgg aagagaagaa ggtcgccctt cgcaagaaaa agcaggagac cagccttcag
     9241 gagatccttt tctgggccta caaaaaggaa tcagacaagc tgctgaagat gatggacgag
     9301 ggagcagaca aggatgccat ccagatgcag gtccagcgtt acgacagcat cggccagatc
     9361 attgcagagg cagacctcga agatgagtat gaggtttgag aatacagcct gaaccactaa
     9421 gaaccaaata ccgaaaggga cttctgaaac agacagaggt ccctttcttt tgctcacttt
     9481 ggcggtcaat tatgaaccaa aaaccagtcg aatgattgtg tacattatgg ctttcaaacg
     9541 acttgataat aatcactttt agagtgatat atgtactaca aaaaacaaaa gactggagaa
     9601 aacaacagat gagcgaatac agaatcattt atatcagcga cgaggacggc ggcgaggtgg
     9661 aaggatgtgg ctacacggct acgatggtta tgatgcggtc agaaacttct tggaatttgc
     9721 ctgcggcatc ttgaaaatcc tgcgggtgcg attcatcaga agagtggact gaaatacaag
     9781 ttagcagata gagaagagag tcaggtttcg acttggctct gttctctgtc tactggcaga
     9841 aatacattga agggaggtga cgactgtggc aaccagagga agaaaatcaa ccccgacagc
     9901 catgaagaag ctggagggaa atccgggcaa tcgcccactc aatgcaaaag agtcaacgcc
     9961 ggaaaagaag gcaccgacct gtccgaagtg gctggaacca gaggcaaaga aggaatggaa
    10021 gcgtctggca aagcagatgg aggccatcgg aatcttgaca gatgtggata tggctgcctt
    10081 tgccggttag tgccaagcct acgcccgctg gaaggaagcc gaggaattca ttacccagca
    10141 cggtatcatc gtgaaaaccc tatccggcta ttggcagcag gtaccacagg tgtttattgc
    10201 ccagacctat ctgaaaatta tgaaccgatt ttccgagcag ttcggtctga cgccttcgtc
    10261 cagaagccgt atcattgccg gtgatggcgg tggtgtcgga gcagaagatg cgatggaggc
    10321 tctgttggga ggtggtagct gatggcagaa accagaccaa agggctatcc caaattaaag
    10381 gaatacacac caaaccgatt catgctttcg gaatgtcact acgacaaagc cagagcggat
    10441 cgggcggtga actttatcgg acagctccga cacaccaaag gcaaatgggc aggcaaccgc
    10501 ttctggctct tgccttggca ggaacagatc atctagtgta atctcgctta actgtattaa
    10561 cggacgtagc gactttatta agagtgttca tggtaggagc attagttgca acaatgattg
    10621 atattaaatt tttatggtac caacgattgg cgatgtgaca ttgaatatgc aaaactatat
    10681 cgatatgata ttatctaaaa tcttactcgc tgggatcgtc gaggtgttta ttggttgtta
    10741 ggaaagaaaa atatgaccca actcgagcga tttttatcgt attacttgtg tgtggcttta
    10801 tctgcattgt gtgtgatttc gaaataaggg agagccaaaa atgagtagaa aattgatatt
    10861 gattagtcac ggaaaatttt gtgaggaatt gaaaaaaagt acaaaaatga ttatgggacc
    10921 tcaagagaat atctacacgt tacctttatt accagaagat ggcactgaaa catacagaca
    10981 aaagctctta ctgtcataga ttcgttaaat gattttgtgg tactatgcga tcttttaggc
    11041 ggtacaccta ctaatatcat ttctaaactg ataatggaag gacaacagat taaactttat
    11101 gtcggattaa tcttcctatg gtcattgaat ttatcaactc gcaagtgatt ggttcagagc
    11161 ccgagtttgt tcgtgcagga tgtgaaagca ttgtatctgt caactaatta attagtggaa
    11221 tagtagaaga tgaaga
//
LOCUS       DAFEZB010000081        10980 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFEZB010000081
VERSION     DAFEZB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.081"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(59..1435)
                     /locus_tag="LP859_002358"
     CDS             complement(59..1435)
                     /locus_tag="LP859_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL3030277.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1563..2681)
                     /locus_tag="LP859_002359"
     CDS             complement(1563..2681)
                     /locus_tag="LP859_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3030278.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2823..3257
                     /locus_tag="LP859_002360"
     CDS             2823..3257
                     /locus_tag="LP859_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3030279.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3335..3868)
                     /locus_tag="LP859_002361"
     CDS             complement(3335..3868)
                     /locus_tag="LP859_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL3030280.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3962..5140)
                     /gene="mutY"
                     /locus_tag="LP859_002362"
     CDS             complement(3962..5140)
                     /gene="mutY"
                     /locus_tag="LP859_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL3030281.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5133..5930)
                     /gene="recX"
                     /locus_tag="LP859_002363"
     CDS             complement(5133..5930)
                     /gene="recX"
                     /locus_tag="LP859_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL3030282.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6037..7419
                     /gene="rlmD"
                     /locus_tag="LP859_002364"
     CDS             6037..7419
                     /gene="rlmD"
                     /locus_tag="LP859_002364"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL3030283.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7593..8171)
                     /locus_tag="LP859_002365"
     CDS             complement(7593..8171)
                     /locus_tag="LP859_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL3030284.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8493..8963
                     /locus_tag="LP859_002366"
     CDS             8493..8963
                     /locus_tag="LP859_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL3030285.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(9071..10297)
                     /locus_tag="LP859_002367"
     CDS             complement(9071..10297)
                     /locus_tag="LP859_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3030286.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3278 a         2190 c         1844 g         3668 t
ORIGIN      
        1 agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa gtttccaatt
       61 atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct tcttcaggtt
      121 ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt cgagaacctt
      181 ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt tcgccttcat
      241 ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca tcactcatat
      301 gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct tggatgatat
      361 attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca tcatcgattt
      421 ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg ccgccatatt
      481 catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac agatcatcaa
      541 taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata tctaaattat
      601 caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt ccgactactt
      661 tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa acttcattca
      721 cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt gggaccatga
      781 cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct aattcttcat
      841 tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc atttttgaat
      901 cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa gcagaaagca
      961 gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa gtgaactttg
     1021 cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca aaaacaatcg
     1081 atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc gctgtgccgc
     1141 cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca ccagacaaaa
     1201 tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga ttctgcaata
     1261 tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg attcgattct
     1321 tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc aatgtcaaaa
     1381 cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca ttcataatcg
     1441 cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg gcactatagc
     1501 cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg tactaaaact
     1561 acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc tttgacgatt
     1621 ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc taagaagata
     1681 ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt taatggatgg
     1741 attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa ctgttggacg
     1801 attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac tgttactaga
     1861 aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt caatcccgca
     1921 atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa aaatgtgaag
     1981 gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa tgttttattc
     2041 agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa ctgttggatg
     2101 tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg tgctgtaatg
     2161 attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt ggatactcca
     2221 ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa atatttattg
     2281 atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct ttctacctgt
     2341 ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag acttgggatg
     2401 acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat tgcgacagcc
     2461 catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa taaatagtaa
     2521 agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc gatcggttcg
     2581 aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa taattcaagc
     2641 gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc tcctttcttt
     2701 cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat ttttgaaaac
     2761 ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa ggtggctaga
     2821 ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat gctgtacgga
     2881 tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa atagacaaag
     2941 atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg caaggaactg
     3001 tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg attctttctg
     3061 aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac tttgctaaga
     3121 atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact ttttacgaaa
     3181 aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct catatttata
     3241 tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa
     3301 aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac
     3361 ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt
     3421 caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg taattcatca
     3481 ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata
     3541 ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg
     3601 aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc
     3661 agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag
     3721 attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata
     3781 ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca
     3841 caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt
     3901 tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa atgagctttg
     3961 cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga
     4021 ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct
     4081 cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt
     4141 tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt
     4201 cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga
     4261 tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca
     4321 tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat
     4381 acgctccaga atgattttga agagctgcac tgatataata aacgtctttt ggctttgctt
     4441 tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt
     4501 ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca
     4561 tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat
     4621 caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga
     4681 ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg atacttgcaa
     4741 tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg
     4801 gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc
     4861 ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta
     4921 gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta
     4981 ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc
     5041 gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct tgaaattctt
     5101 ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta
     5161 atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt
     5221 agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg
     5281 actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc tatcgcttgt
     5341 tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt tctcaataat
     5401 tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg atgtgcattt
     5461 tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa
     5521 cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc
     5581 gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg
     5641 gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt
     5701 tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca
     5761 gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat
     5821 aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct
     5881 acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc
     5941 ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta
     6001 gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact
     6061 ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat
     6121 ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa
     6181 atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc
     6241 aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa
     6301 cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa
     6361 aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag
     6421 ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa
     6481 attgaagctg gtttatacga agcaaactct catcagttag tccctataac agactgcttg
     6541 gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt
     6601 gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga
     6661 atcggcgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg
     6721 caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg
     6781 caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg
     6841 ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc
     6901 ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta
     6961 gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta
     7021 agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct
     7081 gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca
     7141 gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta
     7201 gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca
     7261 aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta
     7321 agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca gactgcacgt
     7381 tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag
     7441 tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa
     7501 tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt
     7561 gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga
     7621 taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt
     7681 cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg
     7741 tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt
     7801 ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta
     7861 atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct
     7921 aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg
     7981 gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct
     8041 gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa
     8101 cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta
     8161 atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat
     8221 taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag
     8281 atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga gaatcaaatc
     8341 atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac
     8401 aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg
     8461 gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt
     8521 gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc
     8581 ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg
     8641 ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc
     8701 acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta
     8761 attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata
     8821 ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg catcatcgcc
     8881 tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt
     8941 attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga
     9001 aaaagtgcca cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa
     9061 ctattttttt ctagtaccca cttgttgttc catactcatt attctggtca taaccataac
     9121 cattttgttc ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt
     9181 actgacttgt atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg
     9241 tgttttctga ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg
     9301 tcgaatcgcc atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat
     9361 taggtaatgt atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa
     9421 gaagatcatt ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt
     9481 gcaactggtc ttgtttgatt gtttcaaacg ccggggtgta gttcgtcgct acatcaagca
     9541 tgtcatccca atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat
     9601 aattgcttac gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct
     9661 gacgacgttg tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta
     9721 aacctttttc tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa
     9781 agtcaatttt attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat
     9841 ccaaattgat cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca
     9901 tggccatttc gacaccacca aatgcgtagg catgatttaa tttatctacc gttccataac
     9961 ctacgatatt cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg
    10021 gattaactgt tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac
    10081 cggtatctaa acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct
    10141 cagaacgttt ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg
    10201 cttctcccat aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acgaataccc
    10261 ctaacaaggt taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt
    10321 ctaaaatatt gtctcatata actgttcaat cagcaaattc actttttaat cataagaaaa
    10381 ctttaaaaaa catttttgtg taacgattat tattttttaa aatttctcaa aattcttaac
    10441 tatcatacca taccgtgaaa aagatcgaaa gaccgaatac acgcagttcg caatcgtttt
    10501 atcttttttt atgtattttt tccagaaaaa gcgaacaaca aggtattttc caaataatcg
    10561 tgcagtaaaa tgatcgcaca tacctttccc atctttttaa cagtctttgc ctaccgttca
    10621 gattatcatt cgatttcacg acaatttctt cctatgactg gtccttttgt taaacccatc
    10681 ttctattcgt tttatttcca gctcactgca tggctcttaa aatgactcga actagtgatt
    10741 gcattaccat catcttttaa tggatagata tgctcctctt caaagtaaaa cgattttctt
    10801 ttaaagtaat agaattgaca tggtaatgag tatgtatact agtggtttca caaatggtct
    10861 tttattcaac atccaaaaac tgaaatttct ttaaaatcca ttactaacga agaaaatgtt
    10921 ccttgcttga taattagtta ggacatgtca agtgaacaaa tgcatttact tagccaaaaa
//
LOCUS       DAFEZB010000082        10436 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFEZB010000082
VERSION     DAFEZB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.082"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            90..308
                     /locus_tag="LP859_002368"
     CDS             90..308
                     /locus_tag="LP859_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030287.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="LP859_002369"
     CDS             complement(597..1109)
                     /locus_tag="LP859_002369"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL3030288.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="LP859_002370"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="LP859_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL3030289.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..4005)
                     /locus_tag="LP859_002371"
     CDS             complement(3562..4005)
                     /locus_tag="LP859_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL3030290.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4006..4794)
                     /locus_tag="LP859_002372"
     CDS             complement(4006..4794)
                     /locus_tag="LP859_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3030291.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4791..5732)
                     /locus_tag="LP859_002373"
     CDS             complement(4791..5732)
                     /locus_tag="LP859_002373"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL3030292.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5909..6496)
                     /locus_tag="LP859_002374"
     CDS             complement(5909..6496)
                     /locus_tag="LP859_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL3030293.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6717..8024)
                     /gene="obgE"
                     /locus_tag="LP859_002375"
     CDS             complement(6717..8024)
                     /gene="obgE"
                     /locus_tag="LP859_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL3030294.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8318..9328
                     /gene="gap"
                     /locus_tag="LP859_002376"
     CDS             8318..9328
                     /gene="gap"
                     /locus_tag="LP859_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL3030295.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9413..10348)
                     /locus_tag="LP859_002377"
     CDS             complement(9413..10348)
                     /locus_tag="LP859_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3030296.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLNQYVH"
BASE COUNT         2888 a         2355 c         1809 g         3384 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     3961 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     4021 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     4081 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     4141 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     4201 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     4261 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     4321 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     4381 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     4441 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     4501 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     4561 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     4621 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     4681 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     4741 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     4801 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     4861 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     4921 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     4981 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     5041 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     5101 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     5161 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     5221 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     5281 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     5341 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     5401 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     5461 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     5521 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     5581 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     5641 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     5701 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     5761 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     5821 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     5881 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     5941 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     6001 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     6061 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     6121 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     6181 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     6241 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     6301 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     6361 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     6421 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     6481 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     6541 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     6601 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     6661 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     6721 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     6781 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     6841 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     6901 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     6961 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     7021 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     7081 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     7141 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     7201 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     7261 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     7321 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     7381 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     7441 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     7501 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     7561 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     7621 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     7681 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
     7741 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     7801 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     7861 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     7921 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     7981 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     8041 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     8101 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     8161 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     8221 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     8281 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     8341 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     8401 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     8461 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     8521 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     8581 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     8641 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     8701 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     8761 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     8821 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     8881 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     8941 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     9001 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     9061 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     9121 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     9181 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     9241 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     9301 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     9361 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     9421 catattggtt taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     9481 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     9541 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     9601 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     9661 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     9721 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     9781 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     9841 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     9901 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     9961 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    10021 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    10081 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    10141 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    10201 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    10261 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    10321 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    10381 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataac
//
LOCUS       DAFEZB010000083         9879 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFEZB010000083
VERSION     DAFEZB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.083"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            16..88
                     /locus_tag="LP859_002378"
     tRNA            16..88
                     /locus_tag="LP859_002378"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Ala,seq:tgc)
     gene            116..189
                     /locus_tag="LP859_002379"
     tRNA            116..189
                     /locus_tag="LP859_002379"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Met,seq:cat)
     gene            203..276
                     /locus_tag="LP859_002380"
     tRNA            203..276
                     /locus_tag="LP859_002380"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Ile,seq:cat)
     gene            300..389
                     /locus_tag="LP859_002381"
     tRNA            300..389
                     /locus_tag="LP859_002381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:336..338,aa:Ser,seq:tga)
     gene            402..475
                     /locus_tag="LP859_002382"
     tRNA            402..475
                     /locus_tag="LP859_002382"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:436..438,aa:Met,seq:cat)
     gene            494..569
                     /locus_tag="LP859_002383"
     tRNA            494..569
                     /locus_tag="LP859_002383"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:527..529,aa:Asp,seq:gtc)
     gene            571..641
                     /locus_tag="LP859_002384"
     tRNA            571..641
                     /locus_tag="LP859_002384"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:603..605,aa:Gly,seq:tcc)
     gene            655..728
                     /locus_tag="LP859_002385"
     tRNA            655..728
                     /locus_tag="LP859_002385"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:689..691,aa:Ile,seq:gat)
     gene            753..840
                     /locus_tag="LP859_002386"
     tRNA            753..840
                     /locus_tag="LP859_002386"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Ser,seq:gct)
     gene            862..933
                     /locus_tag="LP859_002387"
     tRNA            862..933
                     /locus_tag="LP859_002387"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:895..897,aa:Glu,seq:ttc)
     gene            956..1045
                     /locus_tag="LP859_002388"
     tRNA            956..1045
                     /locus_tag="LP859_002388"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:992..994,aa:Ser,seq:tga)
     gene            1058..1131
                     /locus_tag="LP859_002389"
     tRNA            1058..1131
                     /locus_tag="LP859_002389"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Met,seq:cat)
     gene            1150..1222
                     /locus_tag="LP859_002390"
     tRNA            1150..1222
                     /locus_tag="LP859_002390"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Asp,seq:gtc)
     gene            1226..1298
                     /locus_tag="LP859_002391"
     tRNA            1226..1298
                     /locus_tag="LP859_002391"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1259..1261,aa:Phe,seq:gaa)
     gene            1303..1373
                     /locus_tag="LP859_002392"
     tRNA            1303..1373
                     /locus_tag="LP859_002392"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1335..1337,aa:Gly,seq:tcc)
     gene            1386..1459
                     /locus_tag="LP859_002393"
     tRNA            1386..1459
                     /locus_tag="LP859_002393"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Ile,seq:gat)
     gene            1471..1542
                     /locus_tag="LP859_002394"
     tRNA            1471..1542
                     /locus_tag="LP859_002394"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1504..1506,aa:Glu,seq:ttc)
     regulatory      1731..1810
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1808..3001
                     /locus_tag="LP859_002395"
     CDS             1808..3001
                     /locus_tag="LP859_002395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL3030297.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3131..4603
                     /locus_tag="LP859_002396"
     CDS             3131..4603
                     /locus_tag="LP859_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3030298.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4686..5474
                     /locus_tag="LP859_002397"
     CDS             4686..5474
                     /locus_tag="LP859_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL3030299.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5541..6209)
                     /locus_tag="LP859_002398"
     CDS             complement(5541..6209)
                     /locus_tag="LP859_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL3030300.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6324..8036
                     /locus_tag="LP859_002399"
     CDS             6324..8036
                     /locus_tag="LP859_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030301.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8039..9811
                     /locus_tag="LP859_002400"
     CDS             8039..9811
                     /locus_tag="LP859_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030302.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2879 a         1764 c         2253 g         2983 t
ORIGIN      
        1 tataaattta aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag
       61 gtcagcggtt cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg
      121 tgtagctcag ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct
      181 ccgccgcgat ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata
      241 accgtccggt cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg
      301 gaggattacc caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc
      361 gtgcaagggt tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg
      421 gtagctcgtc gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa
      481 aactaaaaac actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat
      541 cgcgggttcg attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc
      601 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct
      661 atagctcagc tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt
      721 taggcccatt agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc
      781 gcccctgcta agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg
      841 tttcaatatt ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      901 gcggtaacac gggttcgaat cccgtacggg tcattaattt aatatagttt tacatggagg
      961 attacccaag tccggctgaa gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc
     1021 aagggttcga atcccttatc ctcctttttc atattatcgc gggatggagc agtctggtag
     1081 ctcgtcgggc tcataacccg aaggtcgtag gttcaaatcc tgctcccgca attaaaaact
     1141 aaaaacattg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
     1201 ggttcgattc ccgtcggaac cgtttggctc ggtagctcag ttggtagagc aatggattga
     1261 agctccatgt gtcggcagtt cgattctgtc ccgcgccatt atgcgggtgt agtttagtgg
     1321 taaaaccaca gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gcttttagtt
     1381 tttatgggcc tatagctcag ctggttagag cgcacgcctg ataagcgtga ggtcgatggt
     1441 tcgagtccat ttaggcccat ttattttatt ggcccgttgg tcaagcggtt aagacaccgc
     1501 cctttcacgg cggtaacacg ggttcgaatc ccgtacgggt catggaagat actttttgag
     1561 tatctttttc tttttatctg aaagaatgat tttaaaaagc ttagcagagt tatctttatg
     1621 gatgattgtg ctaagctttt ttggacagtt ctaatgtaaa atatttttat attttttaag
     1681 caaaaaaatg aaaaaatacg agcagaaaac tgttttatct gctatacttg gttcgttgca
     1741 agttcccgaa aggatccgca ggacggagat gccttgtaac cgaatttaac tagggggaat
     1801 cattataatg gtagaaagac acttatttac atcagaatct gtttctgaag gacatccaga
     1861 taaaattgct gaccaaatca gtgatgcaat cttggatgca attttaaaac aagatccaac
     1921 agcacgagta gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat ttggagaaat
     1981 atctacgacg gcatatgtag atattcaaaa agtcgtacgg gaaacaataa aagaaattgg
     2041 ttatacacgt gcaaaattcg gatttgatgg agatacagca gctgtattgg ttgcgattga
     2101 tgaacaatct cctgatattg ctcaaggagt tgatgaagca cttgagatcc gggatgaaga
     2161 taaaaaagac gtattagatg agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc
     2221 tgtagatgaa acaccagaat tgatgccttt accaatcgct ttgagtcatc gtctggtacg
     2281 acgcttggca gatttgcgta aatcaaatga attaacgtat ttacgtccag atgcaaaatc
     2341 tcaagtaacg gttgaatatg atgatcaagg acaaccggaa cgcgtagata caatcgttat
     2401 ttcaacacag catgatgatg cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa
     2461 agtagtgaag gaagttattc cagctgaatt attagatgat caaacgaaat attatatcaa
     2521 tccgactggc cgatttgtca ttggtggtcc tcaaggggat gccggattaa caggaagaaa
     2581 aatcattgtt gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa
     2641 agatgcgaca aaagttgacc gttctgccag ctatgctgca cgttatattg ctaaaaacat
     2701 tgttgcagca gggcttgctc gcaaagcaga agtacagcta gcttatgcga ttggtgttgc
     2761 tcaacctgtt tcgatctcaa tcaatacttt cggtactgga acggttccgg aagaagaact
     2821 gattgctgca gtgagagaaa acttcgatct tcggccagca ggaatcatcg agatgcttga
     2881 tttgcgtcgt ccgatttaca aacaaacagc tgcttacggt cattttggtc gtacagatgt
     2941 agatttgcct tgggaacata cggacaaagt agatgcgttg aaagcaagtt tagcaaaata
     3001 agttatattc agctggaacg atcgccaagt ggtggttgtt ccagcttttt ttatggcagt
     3061 taacctcttg aaaaagatag taattattta tctattttta gatcctgtca taatgaaagg
     3121 gaggaataag atgaaaaaag aaacaaatgt tactttggtc acagtgagta ttttcattgc
     3181 aacgttcatg acggctattg aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg
     3241 ttcattacat ggaatggaaa tcatgaactg ggtattttcg atttatttgc tgacaaacgc
     3301 tatgcttact ccgatttacg gaaaacttgc ggataaagtg ggacggaaac caatctttat
     3361 ttttgggaca ttattgttta ttatcggttc gttattttgc ggtttatctc aaagcatgat
     3421 tgggttgatt gctgctcgtg cgattcaagg gatgggtgct ggggcaatga tgccagtagc
     3481 tttgacgatc atcggcgatc tttattctat tgaaaagcga gcaaaagttt taggtttgaa
     3541 tagttcagcc tgggggattg ccagtgtttt tggaccgctt gcgggaggat tcatcgttga
     3601 aacgatcagc tggcactgga tcttttttat caatgtgccg atcggaattc tattaattat
     3661 tttgatttgg ctatatttaa atgaaccaaa aatcatacat gagtctaaac caatggatat
     3721 ttttggtagt tttagtttga tggccatgct tgtctcattg ctgttagggt tccaattatt
     3781 aggagacgaa ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt
     3841 aggtattttt gtttttgccg aaaaaagagc taaagatcct gtcatcatga tgcatttatt
     3901 caaaaatcca ctatttgttg tggtcaactt ggctgcagca ttggctagtg gttttttgat
     3961 gggaatcgat gtttatatcc ctatgtggat gcaaggtgta ttagggctga atgcagcaat
     4021 tggtgggctg gtattagcac cattatcact agtctggatg gtcggttctt tccttgcaag
     4081 caagtggatg actcaaatga aaactcattg ggtcttgaga attggactac taatcgcagc
     4141 tattgggggc gcttggttgt atcttatgcc tttcagaagt aactatttat ggttttttgc
     4201 tatatcaacg gtcatcggta taggcttagg tgtgaccatg actacctcga ctgtcagtgc
     4261 acaaaatagt gtatctcgtg accagcttgg tgtagcaact tcttttaata cgttagttcg
     4321 aacaattggt caaacagtca tggtttctat ttttggttta ttgataaatg gggtaactaa
     4381 acgagaactt gcagcagcaa atttaacaaa tgatgcagat atcatgaata aactggtgaa
     4441 tccacaaacg gctaaatata ttgatgcgga tattttgtta ccattaagaa aaattcttta
     4501 tgatgggtta cattccgttt atctagttgg cttgttactc gtgatcattg ctttggtctt
     4561 aacgcttttc gttcatgaaa aaagtggtaa aataaaacag tagtttcggt taagatatgt
     4621 acgtagccga atcattcaaa tttaaagtta ttcaaaaata acttcttact aaggaaggcg
     4681 aaaatatggc atttattcaa gcaaatattt attcgaatgt gttagaaatg gaagtcaatc
     4741 taaatgtgat actaccgcaa acaacaaaaa aggtgattgg caccagttca gagcaagaga
     4801 caacggatat acctgtttta tatttacttc atggaatggg tggcaatcat agtgtatggg
     4861 aaagacgtac gtcgattgaa cgttatgtag caaactacgg attagctgtc atcatgccat
     4921 ctactgatct tggctggtac acagatacag tttatgacat gaagtattgg acattcattg
     4981 cagaagagct acctgagatt tgtcatgaat tattcccgca attaacaaga aaaagagaaa
     5041 aaacatatgc cgctggatta tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa
     5101 aatcagaaaa tttcgcagca gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa
     5161 tggatgatct tttgatggtt cgtaaagcat ctttttggga agggatcttt ggaccattgg
     5221 atcaaatcga aggttcaaaa aatgatccgc aatacttatt gaaagaacta gtaaaaagcg
     5281 gaaaaccagt tccaagattt tatatggctt gtggggaatc tgattttctt tatcaggcaa
     5341 atcaggaaat ggtatctgct atgcaggaac aaggcttggc ggtcaccttt gaacaaggac
     5401 ctggcgatca taactggatc ttctgggacc aatggattca acgtgcattg gcgtggctaa
     5461 tggaagaaaa ataaaaaaag agtcttaaaa agatagctcc tatagagatc acaaacatag
     5521 tgatctctat aggagctttt ttactgctcg ttttctcttc ttttattttg ttggcataag
     5581 ccttcaatat aggtttgtgc actcgttaga aagagtgctt tttcattcaa ttctgcatgg
     5641 gactgcatac tttctttaaa tccctctaat tgattttcaa ctacataatt acgcataaat
     5701 agaactgttt ctttcaaaaa ttcatttccg tccataattt tttgtcgaag ctgctgttca
     5761 tgctgctgat ccattaactg tccaatcaat aaattgtgga gagaggcaat ggcctgatga
     5821 ctttgggtga ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc caaatgttct
     5881 aatcgactcg gaaaagcggg cacagtattg atttctaatt ctgccccaca agggaaacgt
     5941 gtataaaggt catagtaatt ttccatatac tgcagcaact gttcattcca aggcaaagtt
     6001 ggatctggaa gcgctaattc ttcttccatt gtttcagcca ttttttgcaa gagctgttgt
     6061 ttgtttttga aataccaata taaagcaggt gcttgaacat gaattctgtt tgcgatgttt
     6121 cgcatggaaa gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg
     6181 atgattgctt gagaaagctt tatttccatt ttatcaacct cgtgtattgc attatctttt
     6241 taaacatggt aaattgttag tcggtctaat tttacagtgt taaattagtc ctgtcaattt
     6301 aaacaaaggg gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa aaatataaag
     6361 tagctgtttt tatttccttg ctgtccgtag cagtcatggt tgcagcagca ttatggcaac
     6421 ctaagttact acagcaagta ttggaagcta ttattacaga agataatgat gaaatgaaaa
     6481 caattggtat ttacctgatc agtctggctt tgctaggatt agccgcaggt gtaacgaaca
     6541 cgattttctc tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa gaagcatttc
     6601 gaaagatcca gacattttca tttggaaata tcgaacagtt ctctgctgga aatcttgttg
     6661 ttcgtctaac aaatgatatc acgcaaatcc aaaatttagt gatgatttct ttgcaatcac
     6721 tttttcgaat tccattctta tttattggtg ccttcatttt agctatgatc acgatgccgc
     6781 aattatggtg gatcatcgta gcgcttatcg ttacggtatt tctgattacg gcactttcat
     6841 ttacaaggat gggcaagcac ttcatgatca ttcaaaaact gattgatcgt gtgaatagca
     6901 ttgcaaaaga aaacttacta gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc
     6961 aattgaagtc attcagcaaa gtaagtgagg acttaacaaa acacaacatc atagtcggta
     7021 cattattttc tgtgatgatc ccgtccttca tgctggctgc caatttggct gtggttgggg
     7081 cgatcttttt tgtgagcgat cttgttaaag atgatcctac gttaattggt ggaattgctt
     7141 cattcatgaa ctacttgatg cagattatga tggcaatcat tattggcgga atgatgatga
     7201 tgatgacttc aagagcagcc gtatcgctga agcggattgc ggaaatttta gatgctgaac
     7261 cagatcttac ttatttagac gttccagaac aagagttgac gggttctgtc acttttgacc
     7321 atgtttcttt tcgttatcct ggtgaggata cagatacgct taaagatatc agcttttcta
     7381 tcaaacctgg agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag tcaacactag
     7441 cacaattgat ccctcgtcta tttgatccga cagaaggcaa agttgaagta ggtggtgtag
     7501 atcttcgtca agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag
     7561 caattttatt ctcaggaacg attgctcaaa acttgcgcca agggaaaaag aatgcaacag
     7621 aaaaagagat ggaacatgct tcttctattg ctcaagcaaa agaattcatc gaaaagttat
     7681 ctgatcgtta cgaagcacca atagaagaac gtagcagtaa cttttctggt ggtcaaaagc
     7741 aacgtttatc cattactcgt ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca
     7801 gtacgagtgc actggatgct cgctctgaaa agctggtccg agaagcactc gatcgagatt
     7861 tgaagggaac aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc
     7921 gcatcttagt attggatgaa ggcagattag tcggcgaagg aacccatgag gaattggtag
     7981 ctggaaaccg tgtctatcag gaaatctttg aaactcaaaa aggaacggag gagtaagtat
     8041 gactgatcta ataaaagcaa gtaaattttt ctatcactat ttgaagcggt acaaactctc
     8101 ctttttcttt atctttatca cagtgatcat tgctacctat ctacaagtaa aggcacctca
     8161 atacatcggg gaagcttttc aagaattagc taattacgta ggaggcttga tgcaaggcgt
     8221 agacgataaa agcaaatttg tttctgtaat atggaaactc ttgctgtttt atgtactagc
     8281 tagtgcagcc aactttatct atagtatttt gttcacacaa gtagtaggga agtcaacgaa
     8341 ccgaatgcgt atcggtctat tcaacaaatt ggaaaaattg acgatccgtt tctttgattc
     8401 tcatcaagac ggtgaaattt taagtcgttt taccagtgac ttagataata ttcaaaatag
     8461 tttgaaccaa gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat
     8521 catgatgttc cgccaaaatg tagagttagc ttgggctaca attgcttcca ctccagtagc
     8581 cgttttgata gctgtcttga ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga
     8641 tgaagtgggg aaactaaatg gttatatgga tgaaaaaatc agcggacaac gtgtcatcat
     8701 cacaaatggc ttacaagaag aaacgattga aggattctta gagcataatc aaagtgttcg
     8761 cgaagcaacc ttcaaaggac aagcttattc tggtctacta tttccgatga tgcaaggaat
     8821 gtcactggtc aatacagcga tagttatctt tttcggcggc tggttagcat taaatggtga
     8881 tttggaacgt tcggtggcat taggcttagt tgtgacattc gtccagtatt cacaacaata
     8941 ttaccaacca ctgatgcaga tttcatctgg atacagcatg atccaattag cgattacagg
     9001 ggcaagacga ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag aaaacggtaa
     9061 agcatttgat ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg gttattctcc
     9121 agaaaaacta gtcttgaaag atgtctcgat caatgtggac aaaggagaaa tggttgctct
     9181 tgttggtcca acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc gtttttacga
     9241 tgtcaatgat ggttcagtag tcatcgatgg aatagatatc cgagattatg atcttgatgc
     9301 attacgtaga catgtaggaa tcgttttgca agaatctgta ttgttctccg gaacgatcaa
     9361 agacaatatc acattcggca aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa
     9421 acaagcgaat attcatgact tcatcatgac gttagaaaaa ggttatgata cagagatatc
     9481 cgaagaaaat aacttattca gtacaggtca aaagcagttg atcagtattg ctcgaacgat
     9541 catcacaaat ccatctttat tgattttgga tgaagcaaca agtaatgtag acacggtaac
     9601 agaagcaagg atccaaaaag caatggatga agcaatcaaa ggaagaacca gctttgtgat
     9661 tgctcaccgg ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc gtgatgggga
     9721 agtcattgaa gaaggcagtc atcatgagtt attagcagaa aatggatttt atgcggaact
     9781 ttatcataat caatttgtat ttgaaagtta aattaaaaga acctcgttat aacggggttc
     9841 tttttatata ttttatgttg tttgttttta taaggggca
//
LOCUS       DAFEZB010000084         9736 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFEZB010000084
VERSION     DAFEZB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.084"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            86..3364
                     /locus_tag="LP859_002401"
     CDS             86..3364
                     /locus_tag="LP859_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL3030303.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3905..5812)
                     /locus_tag="LP859_002402"
     CDS             complement(3905..5812)
                     /locus_tag="LP859_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL3030304.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5942..6817
                     /locus_tag="LP859_002403"
     CDS             5942..6817
                     /locus_tag="LP859_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL3030305.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6897..7871
                     /locus_tag="LP859_002404"
     CDS             6897..7871
                     /locus_tag="LP859_002404"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL3030306.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8051..9196
                     /locus_tag="LP859_002405"
     CDS             8051..9196
                     /locus_tag="LP859_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL3030307.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9333..9677
                     /locus_tag="LP859_002406"
     CDS             9333..9677
                     /locus_tag="LP859_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL3030308.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3174 a         1797 c         2056 g         2709 t
ORIGIN      
        1 ataattttgt tttttattga cagtaaaaat aaattaggta atactttaag taaacgcttt
       61 ctaatttgtt acgggaggaa tacacttgtt aaaaaaacgt ataaaataca gtgcgctaat
      121 gatgaatctc cttatgctca gtaccccggt tttaatttct gtcgttgaag ttgctgctaa
      181 cgaacaacag tctgtgaacg aagaaaatgc acacgtgaac attgaaggga tgtcaacaat
      241 ggaggaaata ccaagaacta gtgaagatat ggatacggaa gaaggaaatg atcctatgat
      301 gaaagaatca gaaaatgaaa agacaaaaaa gcaatctgaa gaagcaaatg aagagtggaa
      361 aggggttgtt ttcggagaaa gcacaagtgc tagtaggtat gccattgaac cattagaaga
      421 cggcgtgcgg ctctcttcca cgaataacgg cggaaaattc cagtcaagcg gatcagatgg
      481 aatgacctat tattatcaag caatccccaa agacatcaac tttaggatgc gggcgacgat
      541 cgaaatagaa tcttggacct atacgaatgc ccaagaagga tttgctttaa tggtaagaga
      601 cgcagtacca aaaaatcatt tgtttgggca ggcattttat tcaaattcat ttgccatact
      661 aggcagtcgg atcgaatatg tctgggactc ggatagacaa gaagtggtca acacgagcgg
      721 cagcaaatat acgatgcgac ttggattggg gacacgggca atcacaggaa tatcatctga
      781 agatccgcaa gctgcgccag ctcctgggag tgtctcagtg gaaacaacac ctttagagac
      841 ttcagcaggg ttcttggaaa aagaaacagg ttcttataat atattcggaa acggtcatgg
      901 gaatctgttt ccggccacga actcgattac taaactggac gtagagatga aaaaaacgaa
      961 tactggttta gaggcatatt attacgatcc tgaaacagga gaagaaatgg cttctgacat
     1021 catgtatgac tgggaaaaat tatacgcttc agatgaatcg gttatttatg caggatttgc
     1081 tgcagcaagg aatatgacga tcaaggtaga aggaatcgaa gttgcttact cagatcctgc
     1141 gacagatcct cctgggcaag aaagacctac tcgcttgatt gatccgattt acactgtgac
     1201 ctctagtaat cattcaggaa accaagatca tactatgtcc tttagagcaa atgctgacgg
     1261 attattgaca attaaaaata aagcaacagg agaggtgctt aagaacgcaa aaaacttggc
     1321 tatcactgcg aatcgggaat ttgattatca gacaaaactg atggaaggaa cgaacgaatt
     1381 tactttcagt tttacgccag ataaaaacta tccgccagaa gaatacgtag agatgactag
     1441 ttatgaaaca aaagagattc ttcatacagt ggactaccgt aaacctaaat attcgacagt
     1501 ttatgtaaca ccagaagggt cagatgcagg aaacggaagc agacaaaatc cattatctct
     1561 gacacaagca ttgagatacg catatgctgg gcagacaatc gtcatggcgc ctggaactta
     1621 ttccttcgaa agaggattga cgattccaaa aggcgttaac gggacaagag aaaacccgat
     1681 tcagttgatt gcagagaaaa atccagaaaa caagcgtcct gtacttgatt ttaaaggaac
     1741 tggaaatggg atgacactgt tcagtcacta ctggggttta cgagggtttg acattacaaa
     1801 tagtgcagct atgcaaaaag gattacagat ttctggaaat cataatttga tcgaagagat
     1861 tcatgcttat cggaatggaa atacaggaat ccaaatcagc ggtagctcaa atgatccttt
     1921 cgaagcatgg cccgcgcata acttagtcaa gaactgtaca tcttttgaaa atgccgatcc
     1981 aggatttgaa gatgccgatg gatttgcggc caaactgaca ataggagaag gaaatgtatt
     2041 tgacggttgt atcgcgtatc ataatgcaga tgacggttgg gacctttttg ctaaaaatga
     2101 aacaggttcg atcggaaaag taatcatcaa aaactcagtt gcttatcgaa acggctgggt
     2161 accaggaatt gagggagaag gtaacggtaa tggattcaaa atgggcggtt cttctttaac
     2221 gggccctcat gaattgatca atagtttgtc tttcgaaaat cttgcgaaag gaatcgattc
     2281 caatagcgga acagatatta tcgtcaatag cagtacaagt tttaataacg gttcatataa
     2341 tgtggctctt tatacctctt cagcaggaaa cacagactac catgcttctg gtatcttatc
     2401 tttccgaaca gagaatcttg agatgagaga gcagatccgc ccattgaatc aaaacgaaga
     2461 tcaaatctac cactcgttga actactattg gaatgaacga gagaaacaat cagaaaatac
     2521 tcttggacat accatatcaa aagattggat cgagtctcta aatacatgtt cgaaagaagg
     2581 tcagcagcct gttacgcgct atgaaaatgg aagtatccat gttcatggac tgatgcagat
     2641 taaaccgggg aatcgtcaaa cagaaaagga aagagtggat ggtcttccat tatctgtagg
     2701 tgctattttt gacggagaaa cctcaccgtc ttcggaatat ccagagtatg caatcgtaca
     2761 agaaccaaat cagacaaaac caaatgaaca aaataatccg attattatac aagaagcaaa
     2821 tggtgtggcg gagaatgcag aaggaaatat cattgaactg aagcctttta cagtttttgt
     2881 gtcaactttc acaccaaatc aaggaacgcc tgtctttgaa gatatgcgtt atttcccaga
     2941 aaagatccaa atcgttgaaa cacagtcaag tgctcaattt tctaatcata ccgctatttt
     3001 ttcagcacct tcaaaaggag aatatacgct agaagtcact tatcgttcgg aaatatttga
     3061 tggggaaaaa tggtctgctc atacagaatc tgttacccag caaaaagcga tcactgtgcg
     3121 agaaactgcc gatgtgcccc cgggaaatga caaaccagaa agagaaaatc caggaaatga
     3181 atcaccaaat ggaaaaccaa atggaaaacc ttctcatccg gataggacag caaaagaaag
     3241 tgaaaaaaat ggcactcaag gactcccaaa aacgggagaa tttaataacc cgttgctagc
     3301 attagctgga ggaattttgt taatcggtgt agttgtttat gtcatgaaac agcgaaaaaa
     3361 ataagtggaa tttctccttt tatgatgtaa aacaattgac tgaggaaagt aaaaatagaa
     3421 ggaaatgaga ccaatagatg tctcacttcc ttctatcttt ttatagatga ggttctcctt
     3481 gctgcttttg attggctgtt cttattactt gatgcaggac agctttttca caatctctcc
     3541 actaaacggt gttcaatcag ctgaccctcg gtattagctc aacaaacggc aaaatatgag
     3601 gaactatttt tccgtttttt tctctaatac tcaggtcagg acgctgattt cacaacctct
     3661 cgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg
     3721 gaaagccatt ttcacaaaat ttttcttact acttgaaact aagcagcttt ttcacaacct
     3781 ctaatacatg gtattccaga agtaacttct tcgaaaaata agccgaaatg tcacaaaaat
     3841 ttgagaagcg attttcccaa attccttctt atttcttgaa gctgaccact tctggcacaa
     3901 cctcttacca gattcgcact cttttttctg gcgctagata catagcgtct tgttccgtga
     3961 taccaaatgt ttcatagaat tcgtcaaggt tttttggttg cacgtttgct cggagtttag
     4021 caggggcatg gacatcgatt tgcagtaaaa gttgttggta ttcttttttt gcttttgtac
     4081 gccaaatcgt tgcccaattg acaaagaatt cctcgataga aagatctgat tccgtttttg
     4141 ctgcttccaa tgcacagctt agaccgcctg catcagcaat attttccgaa acggttaatt
     4201 ttccgtttac ttttccgtca gcaaaaggca gaccatcgaa ttcttcgatc attgcttgtg
     4261 ctttgttttg aaattgtgta agatcttctt ctgtccacca gttgttcaag ttgccatatt
     4321 cgtcaaataa agcgccgtta ttatcaaatg catgggaaat ctcatgagcg ataacagctc
     4381 cgattccacc ataattagca gagcttgatt gttctagact gtaaaatggt gcttgaagga
     4441 tcgctgccgg aaagacgatc acattccgaa atggatgata ataagcattg accgtgtcag
     4501 cactcatttc ccattctgaa cgatccactg gtttgttcca tttgctgaaa cgatctttta
     4561 atgtgagttt gctgaaaaca agagtatttg aaagcaaagt accaccctga tctgcaggta
     4621 ctgttttgaa ctggtcgaat aatgctggga tcttatcggg gtagcctact tggataccta
     4681 agttatttaa ttttatgacg gctttttctt ttgttgcatc cccaagccag tcattttctt
     4741 ctagacgttt tttataaacg gcgatcattt ttttcaccat ttgttctacg tcttttttag
     4801 cagcttcgcc aaaatatttt tttccgtagt aatcgccaac tacttgatcg aattgtccag
     4861 aggcaaggta atacgctgct tttttctgag gcattgcttc gtctgttcca gacaatacgc
     4921 gagaatagat tccgctgact tggcggaatt cttctgatag ataaccacta agagaacgta
     4981 ctgttttaat gatcatccag cttttgaata acggaaaatg agcttctgtc aggatgtcat
     5041 tcaaatgatc gaagtacact ggatcagtta cgatcacttt atctggagtt gtcccaatca
     5101 aacccgtgac aagttttttc aagtccagtt tatctgtgta tttaataaat gtatcaaaag
     5161 attgcggatt atacattttg ctgtaatctg cgttttcttc tgcacttttt acatgaggag
     5221 caatcaattt atcaaattgc actgcttgtt ccgtgattgc ttctgcctga cttttttctt
     5281 tgccggcaag ctctaataac tgaacagcca tatcaaagaa aacgcctatt agttgtgcac
     5341 cgttgggatg gtctgatgca taatacgtct tatctggaag gaataaagaa ggcggataag
     5401 caaagaaggc gttcgtttgc gcattcttca tatctgcatc tacatccaga ttaaatggca
     5461 atggtaatgt atccaacagc cattctggaa attgcttatt caattcgtcg aaagactgca
     5521 atgattcaac tttttcgatt ataggttgaa gcggttgagc atgcaactca tttcttcggt
     5581 cataatcgtt tgccagttga taatatgcaa ggaaatgttt cattttttct gaagacactt
     5641 tttctggatg ggcaagcaga tcgtctgttt ctcccatcaa aagttggtcg atcccgtcaa
     5701 ctaaatcttg gaaaccgcct gtagctggtt tatctgcagg gatttttgct gttttttgcc
     5761 attcttcatt gactgcttca tagaaatctt gtttcaatcg ttctttgttc attcatcgat
     5821 cgctcctttt ttgtttcgtg tgcccattat cttacaaaat gaaggaatta acaaatagaa
     5881 cataaaaaat tgaaaattcg aacaatcaaa ctgatttgtt tttttcaatc cttcacttat
     5941 aatgaaaaaa aggaggcgat cccaactgaa tcaagtagtg gataaacctt tatatttcaa
     6001 agtagataaa aaaattaaaa aattagccag cactcagcag ctgcagtcaa gaaaaagtaa
     6061 attgcttttt ttagctctgg tttttgaaga tcaaagttat gtgattatcg atcaatcagg
     6121 acatccggta gaatattctc cagcaaaata tacttatcaa gagggtcttt cttgtaaaaa
     6181 atggaagctg atcaatgaag agccaatcga gttatctaga tggatcaaca gaaaagaaga
     6241 tattcccgtt ttgatcgaag aaaaaaggtc aggaaaggaa ctcgcaaatt gctgggtggg
     6301 attgccagaa gaacgttttt tgaggtataa gaaatgggct accccctcag gctatctttg
     6361 tggtacttac gcggcagcag ttctccttgc ctattatcaa gactatcgta aggggtggat
     6421 gcttccaaat gagattcgca agaaaaatac ggctgattcg ttggttttga cgaaggcatt
     6481 gcgtagccag atccaaccac taggacttcc tacgattcct tttcaggtta gtacagggat
     6541 ttctagtttt ttaaagaaga atggcaatca tgagcgtgca cgagcaactc ttctaggttc
     6601 atggcagcga gctacaaaac gtatacgtga aggcaaacct gtgatgattg gtattttgaa
     6661 agtacttggc agtacatatg gcaatcattg ggtgacagcg tacgcttatt ttgaaactga
     6721 aacaggagaa cgttattaca aagtacacga caactggggc gattatcata aagtcattcc
     6781 tgccagttgg agtaacggaa cagtttcact tccttaagaa tttgctgaaa ttaacagaaa
     6841 atcttggaaa aatatgttaa actatcgaag aatagagaag ttttggggag gactacatga
     6901 gtaataagta tcaggatgac acgttgaaga tttttagttt gaatggcaac cgtccattag
     6961 ctgaaaaaat cgcgaaagtg ttcggaacag aattaggtaa aagcgtcgtg aagcaattta
     7021 gtgatggcga gatttccatc aatatcgaag aaagtattcg aggggatcat gtgtatatcg
     7081 ttcaatcaac gaatgcacct gtcaatgatt actatatgga actactgatc atgatcgatg
     7141 caatgaagcg agcaagtgcg aaaacgatca atgtcgtatt gccttactat gggtatgcta
     7201 ggcaagaccg cacggcgaaa cctcatgaac cgatcactgc gaaattgatc gctaacttga
     7261 tcgaagaagc aggggcaact cgagtactga ctttagatct gcacacggtt caagtacaag
     7321 gattctttga tattcctgta gataatttat ttacgatgcc gttgtttgct cattattacc
     7381 gtcaattagg tctgacggga gatgacatcg tagttgtttc gccaaaaaac agcggtgttc
     7441 agcgtgcacg tagtctttca gaatatttga acagcacatt agccatcgta gatcatgcag
     7501 atgatgcttc aactgagggt ggttatgtga tcggtgatgt tcaaggaaag acatgtatca
     7561 tggtagatga tatcttgaac acaggagcaa cgcttgcacg agcagcaaat gtattaaaag
     7621 aaaacggcgc aaaagaagta tatgcctgtg cgtcacatgg attattgtcc gatcatgcga
     7681 aaagtatttt agacaatgca ccaatcaaag atatttgtat cactgattct gttttcacag
     7741 aagaaaatcg tcatccagaa aacttaacaa tcgttacatg ttcagaatta atgggtgaag
     7801 cggtcaaacg catccatgaa aatacaccga tgagtccgtt attccgtctg gaagaaaaag
     7861 aattcgaata aacaaaaaaa agagggaaag aagttgtgac agaagtgagc agcttcaagg
     7921 agctgcgaca agacttttgt cacagctcct tttttctatg cccatgaaac gtcctctaga
     7981 caaaaatgag ctcttctttg gtttgttcgc tattttatgc tagaatgtgc ctagcaataa
     8041 tttggaaggg atgaatgaag tgaaaactat ttatctggat catgcggcaa ctacccccat
     8101 gcatccacaa gtaattgaag cgatgacggc aatcatgcaa gacacgtttg gaaatccttc
     8161 aagcatacat ggatttggtc gccacgcgca tgaaaaattg gaaacagcaa gacaaatcat
     8221 tgcagacagt ctgcaagcaa aacctcacga gatcatcttt aacagtgggg gaacagaagg
     8281 ggataataca gctatcttag aaacagcgtt ctcgcgtcaa aaagaaggca gacatctaat
     8341 cactacagct atcgaacatc ctgctgttct taataccatg aaatatttag agacaaaggg
     8401 gtttgacgtt acatatctgc ctgtagatga aaaagggaat ctgtcgatcg aagaggtcaa
     8461 aaaagcgtta cgagaagata cgatcttggt ctctgtgatg tacgggaaca atgaaatcgg
     8521 aaatttgatg ccaatcaaag aaatcggtga acttttagcg gatcatccag cttatttcca
     8581 tacagatgcg gtacaagcgt acggaaacca aacgatccat ccagaagaat taggaatcga
     8641 tttattaagt atctctgctc ataaaatcaa tgggccaaaa ggcgtcggtt tcttatataa
     8701 acgagacgga gtaaatatcc ctgcattgct tttaggcggg gagcaggaag aaaagcggcg
     8761 tgcaggtaca gaaaatttag cagggatctg gggtatggca aaagcagttg aactgctgtc
     8821 acctgaagaa cgtgaagcac gtaacaacaa atataaagga ttccagcaat tgattcttga
     8881 tacattagaa gcaaacgaaa tcccttatca agtaaatggt gatttaacga ataaactccc
     8941 tcatgtgttg aatcttcgct taattggcga agcaaatgat ttgctactga tgaagcttga
     9001 tcttaatggg gtcgctattt ctactggttc agcttgtaca gcaggaaata tcgagccttc
     9061 acacgtatta gaagcaatgt atggagaaga tacaccgata ctaaaagaat ccatccgtat
     9121 cagctttggt ttgggaaata ccgaagacga catccagtat ttccttgatc aattagttcg
     9181 tgctgtcaaa aaataactag tgaagtcatt cttatatgaa cagatgtctg agagaacgcc
     9241 ttcatttttt agggaactta actgaaaaac ctagtaaaaa tcaggataaa cttttataat
     9301 aaagagaaac taattttatc gaggtgaaaa tcatggcatt tgaaacaaca gcaacggtac
     9361 tgggcgcacc aattagctat cgtttgcatc ctgatgcaaa aagatataca ttgcgcgata
     9421 atggatttac agaaacaaac ggaggcaatt atcaattgat ccgaccatta gatgccacgc
     9481 cgcaaagcaa agaaggattc aaattaaaaa tcactgtatc aaaagatatt caaacattga
     9541 aaatgtcgat cactacagca aatggattga aagcagtcaa tattttcaaa gatccaaaac
     9601 agaaaatgag ccaagataaa ttttattttc tgatggatgg gatgattagc cgtgggttat
     9661 tcgaaaaagt cgaatgatct gtggatataa aaagacaact agttttgaaa cggcctattg
     9721 ttagatgact aacttt
//
LOCUS       DAFEZB010000085         9725 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFEZB010000085
VERSION     DAFEZB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.085"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1017
                     /locus_tag="LP859_002407"
     CDS             <1..1017
                     /locus_tag="LP859_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3030309.1"
                     /translation="EGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLN
                     KEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVT
                     DTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKG
                     TVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDG
                     SQYHGTADKNGNFTVKVPKLEAGTKVRVTATDESGNTSEPTNVVVSSNKKDSGKNGSK
                     GSKTDNQDGNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1151..2494
                     /locus_tag="LP859_002408"
     CDS             1151..2494
                     /locus_tag="LP859_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3030310.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKLILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GKEKIEKIVTTRDNKYAMDMVIQAIGFLSHSPIGKKEFLRDVSGAYLTDEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINDQSFTPSGSQFTNGFSIFG
                     YNFYAAGLNLKTARLLSEAIDYIEIEDYIRPIFSKTNNIVKLRISFEKKAKRIVGVQF
                     YSQEDLSGLLTMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNSGA"
     gene            2606..4009
                     /locus_tag="LP859_002409"
     CDS             2606..4009
                     /locus_tag="LP859_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL3030311.1"
                     /translation="MKKSLKLASAVLLMSHLLIATPFHVLASENSTYKIAKERTKTTE
                     MSNSDVTAEGSEFSTTSSSESSTTLNAESSTTSSSESSMTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTSQQIESTETFQSQPV
                     LSGNEEIHFDKNESVENFIMKIGDSARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGSFVIMVTNEDLGNGTLYKTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNGLIKTYDLTDYDKEVQGP
                     QMNKNGYQIPIHNYSISSLFGNRGDEFHRGLDMAAAQGEPIHAVKAGKVIKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVVQGQMIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGN"
     gene            4427..4642
                     /locus_tag="LP859_002410"
     CDS             4427..4642
                     /locus_tag="LP859_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030312.1"
                     /translation="MNQEIADIKLIKEVLQESTANAIASGSGISLSTAKKLKSGERNV
                     EKLNLSDAIKITEFAIKNRHVKIEIWK"
     gene            complement(4728..5639)
                     /locus_tag="LP859_002411"
     CDS             complement(4728..5639)
                     /locus_tag="LP859_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3030313.1"
                     /translation="MKIKIERNQKEKSPKKNKERVISVGKHRKMVLALWLLLSCSLAF
                     GIYRNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWRNNKEAIEKRMSNL
                     EQYLTENGLALSQDMVRADIPTSSEVQSIKILDVEKHSEEFIVSFLVEQQITEGKKTQ
                     SISSAYRVTIFEDENGSHIVTSLPTMIAKPEKAKYESKQIGIESDIDAKTTEEVTDFL
                     ETFFKLYPSASEKELEYYVENKAVQPINSNLKLVELVNPTFKLKGKEIQGEIFVKYLN
                     NTTKVSNIFEFTLTLQKVKNWKIIDIG"
     gene            complement(5658..6662)
                     /locus_tag="LP859_002412"
     CDS             complement(5658..6662)
                     /locus_tag="LP859_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL3030314.1"
                     /translation="MNVKKLSIISLTILTLSFMVVLMCVGLLFGEDSEGSNSSGASSG
                     GSNVSEEVLKHRAMVEKYAKESNISEYVLILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVAKNGKKHNFALA
                     ESFSKEKSSGQKVDYPNPIAIKKNGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGASPETGFDCSGLTQWCYAKVGLKLPRVAQDQYNAMTHIDVKDA
                     KPGDLVFFHSTYDTGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(6659..8782)
                     /locus_tag="LP859_002413"
     CDS             complement(6659..8782)
                     /locus_tag="LP859_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3030315.1"
                     /translation="MKKKLLRIVGIIAISLCTILLLLSLIGTVAEATGLVDDTVKSGN
                     LYSQYSLDNYQLDFFVDSSWGWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQVSGFYFGFLLLMILALGVYVAYI
                     GLLKREATKAVRAVINFVTIFLLSGSFIAYAPTYITKINNFSSDVSEAALSLGTELVV
                     PNSESKGKDSVDLIRDSLFSIQVRQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRIMRKPHMLMNRKLR
                     QLNRNVIRTLAFGGGAAVGHKLEKEQSKSKFKPSGSSLRKNSRLPNDHEVPSDSTKEN
                     PVSNNKKQSRMNLAGRKMGKVLDTQALVKDKTKQVRDQVRNTPTNLKYNFHKGIEKTK
                     KAPKEFKRGLVQEKANRAELREKQRQHRDEKMAEKRKVLNETGNRHKKRRENVSIKPE
                     RQPQKDRAPKKEAPKQIVRVNPNPEIKRKLSSHEIVSKRISQPSSKKNSFQQVKQRST
                     LPKRTNQKVQKRMNPRPKSRSGEKK"
     gene            complement(8787..9698)
                     /locus_tag="LP859_002414"
     CDS             complement(8787..9698)
                     /locus_tag="LP859_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3030316.1"
                     /translation="MASEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLT
                     GISSRDGEKFPVLRKAIRTVTQSNQRGLLQVITELRNDGTPVAENIADHIESFTDYDF
                     AHLLFSDGSITQSISLEKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFA
                     LDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLD
                     ERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDESNQKRLRDLENGQCLMQDLYGRV
                     GVVQVHPVFEELFHAFDTRPPVQKRME"
BASE COUNT         3272 a         1740 c         1715 g         2998 t
ORIGIN      
        1 gaaggtgtca ttgaagtaac gtatccagac ggtacgaaag atacagtgaa agttccagta
       61 gaagtgacag acaatcgttc taatgcagat aaatacaatc ctgaaggaca aaaagtgacg
      121 acagacttaa ataaagagcc agatgcatct gagggaatta agaacaaaga agatttacca
      181 aaaggaacta cttatgcttg gaaagagaaa gtagatgtta gcacagctgg aaacaaaaaa
      241 ggtactattg tagtgacata tccagatgga tcatctgatg aagttgaagt agacgttaca
      301 gtgacagata ctcgttccga tgcagataaa tacaatcctg aaggacaaaa agtgacgaca
      361 gacttaaata aagagccaga tgcatctgag ggaattaaga acaaagaaga tttaccaaaa
      421 ggaactactt atacttggaa agaaaaagta gatgttagta cagctggaaa caaaaaaggc
      481 actgttgtag taacatatcc agatggatct aaagaagaag tagaagttac tatttctgta
      541 gtagataaaa aagcaccgaa taaacctcaa gttgatccta ttacagaggg tgaccaaatt
      601 gttactggta aaactgaacc aaatgcagag gtaacagtta cattaccaga cggaagtcaa
      661 taccatggga cagctgataa gaatggtaat tttacagtta aagttcctaa attagaggca
      721 gggacaaaag tgagagtaac tgcaacggat gaatctggta atactagtga acctactaat
      781 gtagttgttt cttccaacaa aaaagacagt ggaaaaaacg gtagtaaagg tagtaagacg
      841 gacaatcaag atggcaatag caatcaagac aaaaatagag gcaaaagcca gtcctcaaaa
      901 gtttttccta agacgggaga gagtgacagt aatatcttta ctattagcgg tggtttaata
      961 ctgttaggaa cgttaggatt attagggtat aaaaatcgaa aaaaagagaa tgaatagaaa
     1021 atcaatttct taagtcttct ctgagaagag gaagaatctt tttcttcctc tttttgcttt
     1081 tttatgatgg gaaataagtg ttagttaaag atgaatatac tttttataga aaggaaaaga
     1141 atgtaattaa gtggaaaaaa tagtaattat tggttcaaac cattcagggc tagccgcagc
     1201 tcgatttcta aacttaagcg attcaaatta tgaagttatt ctaatcgata aaaaatcaga
     1261 aattagttac ttggaatgtg ggacatcgtt atttataaaa ggagaagtaa gtaatttaga
     1321 agatcttttt tatcaaaata gaatgacact aaattctgag ataaaaaagt tatttctaga
     1381 gacagaagtt gacaaaattg atattcaaaa taaaaagatt agttgtgtaa aaaaagatgg
     1441 cacaagattt tcccaagaat atgacaaact tatattagct acaggttcaa agcaaattat
     1501 gttagatata ccggggaata atttagctaa tattttttcg ataaagaatt ttccaaatgc
     1561 caaagagatt ttcaaagctt tagatgataa tactattaaa aaagtaggta ttattggagc
     1621 aggatatatt ggagttgaaa ttgctgaagc tataagaaaa agagggaaag aagtatatct
     1681 tttcgatgtt gcagatagag tgttgtcgac ctattacgac cgtagttttt cagataaagt
     1741 tgaagaaata ctatcaaaaa acgggataca tctttgtcta tcagaaagtc ctaaaaagta
     1801 tttaggaaaa gaaaagattg aaaaaatagt gactacaaga gataacaaat acgctatgga
     1861 tatggtcata caagctatag gattcctatc tcatagtcca ataggaaaaa aggagttctt
     1921 gagagatgtt agtggagcat atctaacgga tgaataccaa caaacaaata taaaagacat
     1981 atatgctatt ggagattgtg ctacaacttt ttttacatcc attaatagaa gtagcgttga
     2041 tttttctatc tctaatgctc taagaacggc ttatattgca actcaacata ttaacgatca
     2101 aagttttaca ccttcggggt ctcaattcac aaatggcttt tctatatttg gttacaattt
     2161 ttatgcagca ggtttgaatt taaaaacagc acgcttatta agcgaggcta tagattatat
     2221 agaaatagaa gattatatta gaccgatttt ttctaaaaca aacaatattg taaaattaag
     2281 aattagtttt gaaaaaaaag caaagagaat agtaggtgta caattttata gtcaagaaga
     2341 tctatctgga ttattgacaa tgttctctct tgctatagaa gaacaaatta cattagacaa
     2401 attgaaaatg ttagattatt tatttcatcc tagttttagt caaccctata attttatttt
     2461 aaaggcagta acaggattaa atagtggagc ataatcgtta ttaaatgaaa tcaagaaata
     2521 tactgtatta ctcaattcat tgaaaatatt tgtcatattt tttaatagaa aacactatta
     2581 aagaaaaaaa agagaggtaa atcaaatgaa aaaatcttta aaacttgcat cagcagttct
     2641 attaatgtcc catctcctta tagcaacacc tttccatgtg ttggcaagcg aaaattcaac
     2701 atacaaaatt gcaaaagaaa gaaccaagac gactgaaatg agtaattccg atgtaacagc
     2761 agagggtagt gaattttcaa caacttcatc aagtgaaagt agtacgactt taaatgctga
     2821 gtcttcaaca acttcatcaa gtgaaagtag tatgacttca aatgccgagt cttcaacaac
     2881 ttcatcaagt gaaagtagta cgatttcaag taccgaatct tcaacaactt caagtagtac
     2941 aagtgaaata acttcttcga aagttacaaa gccaaaccat atagagtctt caaaaaagga
     3001 agcgccaaaa acggaaggac aaacaaaaac tttttcgaca tcacagcaaa tagaatcaac
     3061 agaaactttc caatcacaac cagtactatc tggtaatgaa gaaattcatt ttgacaaaaa
     3121 tgagtctgta gaaaatttta ttatgaaaat tggtgattct gcgagaaaaa ttggtcagaa
     3181 atatgattta tatgcttctg ttatgattgc acaagcaatc ttagaatctg ccagtggtca
     3241 aagccaatta gctcaggcac cgaattacaa tttatttggg ataaaaggaa cttataatgg
     3301 aagctttgtc ataatggtaa caaacgaaga tttgggtaat ggaacgcttt ataagactca
     3361 atcgaaattt agagtgtatg aaaactatga ggaaagtttt gaagattatg ccaaattatt
     3421 aactaaggga attagtggaa ataaagattt ttatgccgga gcattaaaag ctaatagtaa
     3481 aacatatcga gaagcaacta aattccttac aggacggtat gcaacagata ctcaatacta
     3541 tttaaagcta aatggattaa ttaaaaccta tgatttaact gactatgaca aagaagttca
     3601 aggaccacaa atgaataaga acggatatca aatacctatt cataattatt caatatctag
     3661 tttgtttgga aatcgtggag atgagttcca tcgaggactt gatatggcag ctgcacaagg
     3721 tgaaccgatt catgcagtta aagctggtaa agttattaaa gcagagtttc actattcttg
     3781 gggaaattac gttgttatac aacacgaaga tggatcaaca gcgttatatg ctcaccaaca
     3841 acaatactta gtaaaaactg gagacaacgt tgtgcaagga caaatgattg gctatgtagg
     3901 aagtactggg aatagtacag gaccacacct ccatttggaa atttgcaaag atagtagtct
     3961 ttctcaaagc atgttagttg atccgaaaac cttattattt gggaactaaa aagaaaaaga
     4021 gcattttatt agaagtatgg attagggaat atagtagtag tgggctctaa agattatgaa
     4081 atctttaaga gctcttttta agacgattgt ttaaaacgaa ttaaccaagt aggagctttc
     4141 ctagaattta taaaattata gtttcttgta tgacatgtat atagcatgct aaaataccaa
     4201 taaataaggt ttaagataaa cgcaaaaagt cgaaaaatta acacttattt tgtgatatta
     4261 attttaattt gcaaaacaag ctatatggaa aaagtttaaa ctttttctag agtccctatt
     4321 actagttaaa aagaccactg tagtcctctg ataaatcatc ataaagacca cagtagtccc
     4381 cttaaagttt ttccaaatta actatctgag aaaggagaga agatttttga atcaagaaat
     4441 agctgatatt aaattaatca aagaagtttt acaagaatct accgcaaacg cgatagcatc
     4501 tggatcaggg attagcttaa gtacagcaaa aaaactaaaa agtggcgaga gaaatgtaga
     4561 aaaactaaat ctttctgatg caataaaaat cactgagttt gctattaaga ataggcatgt
     4621 gaaaattgaa atttggaaat gacggataca actattatag ttttgttttt tctaagtatc
     4681 atatttatga atatgctttt gctaaacatc taattataga ttaattttta tccaatatct
     4741 ataattttcc aattttttac cttttggaga gtaagggtaa attcaaagat attgcttact
     4801 ttagttgtat tattaagata cttaacaaaa atttctcctt gaatttcttt tcctttcagt
     4861 ttaaaagtag gattaaccaa ttcaactaat ttcaaattac tattaattgg ctgaactgct
     4921 ttgttttcaa catagtattc aagttccttt tccgatgctg atggatacag tttaaaaaag
     4981 gtctctaaaa aatcggtgac ttcttccgtg gttttagcat caatgtcaga ttcgattcct
     5041 atttgttttg attcatattt tgccttttct ggctttgcaa tcatagtagg caaactcgtt
     5101 actatatggc tcccattttc atcttcaaag attgtcactc gatatgcaga agaaattgat
     5161 tgtgtctttt ttccctctgt aatctgttgc tcgactaaaa atgaaacgat aaattcttcc
     5221 gagtgttttt caacatctag aatttttata gattgaactt cggaactcgt gggaatatct
     5281 gctcgaacca tatcttggct taaagcgaga ccgttttctg taaggtactg ttcgagatta
     5341 ctcattctct tttcaattgc ttccttgtta tttctccatg aaaaatattc tttcgcaaaa
     5401 tcctctgtaa aactttcaac gcctgaagta ttcactacct tctcttttat aactactttt
     5461 tcatgagtcg tatgctgatc aatggctgtg aaatttctgt agattccaaa agcaaggcta
     5521 caagacaata aaagccaaag agcaagcacc atttttcgat gcttgcccac tgaaatgact
     5581 cgttctttat tcttttttgg gctcttttcc ttttgattcc gttcaatttt tatcttcatg
     5641 ttttttcctc ttttctctta tttgtaccgt ccagcaccga taagatgctg ctgccaatag
     5701 ctttctgtta gatttgtcca gcccactgga tctcctgcat gaaacatgcg attctctcct
     5761 gcataaatac cgacatgggt cacataagta ccggtatcat aagtcgaatg aaagaaaacc
     5821 aaatcacctg gtttggcatc ttttacatca atatgggtca tggcattata ctgatcttga
     5881 gccacccgag gaagtttcag accaactttg gcataacacc attgagtcaa gccactacaa
     5941 tcaaacccag tttcgggact tgctccacca aacacataag ccgttccttc atacttaaac
     6001 gcctcatcaa aaatcccttg gactgtttta tcatcaaact gtgttgtagt taggtattgc
     6061 ttcactaata agcaataaaa catattgcca tagttatacc gccacccacc atttttctta
     6121 atcgctatgg gattagggta atcaactttt tggccgctcg atttttcctt tgaaaaagat
     6181 tcggctaaag caaaattatg cttcttcccg tttttcgcga catgatccag atacccgcca
     6241 ccatagttat acgcttgaat aatgctatct tgatcacacc ctagttcttt ggaacgctta
     6301 accaaagacg caaaatagag acatccttgt ttgatggatg cctcggtact taatgaattc
     6361 ggtggcaacc cggctgactc cgaactctgc atcacatctt ccattgtgcc accagattcc
     6421 acttgaataa tcgccaaaag aatcaggaca tattctgaga tgtttgattc cttggcatat
     6481 ttttccacca ttgctcggtg tttcaaaact tcttctgaga cattacttcc cccagaagaa
     6541 gcacctgaac tattacttcc ttcactgtct tccccaaaca acaagcccac acacatcaag
     6601 acgaccataa aggagagtgt caagatggtt aacgaaatga tacttaattt ctttacattc
     6661 attttttctc acccgacctt gattttggac gagggttcat tcttttttgt actttttgat
     6721 tggttcgctt gggcagagta gacctctgtt tcacttgttg gaaagaattt ttcttacttg
     6781 atggttgact tattcttttt gaaacaattt catgactaga tagttttcgt ttgatttcgg
     6841 gattcggatt tactctcaca atctgttttg gtgcttcttt ttttggcgct ctatcttttt
     6901 gaggttggcg ctctggtttt atcgaaacat tttcccttct tttcttatga cgattaccgg
     6961 tttcgttcaa tactttgcgc ttttcagcca ttttttcatc tctatgttgt cgttgttttt
     7021 ctctcaactc cgctcgattt gctttttctt ggacaagtcc gcgcttgaat tctttcggtg
     7081 cttttttcgt cttttcaatt cctttgtgga agttgtattt caagttggtc ggcgtattcc
     7141 ttacctgatc ccttacttgt ttagtcttat ctttcaccaa agcttgggta tccagaactt
     7201 tgcccatttt tctcccagcc aaattcattc gggattgttt cttattattt gaaactggat
     7261 tctccttagt cgagtcagat ggtacctcgt gatcgttagg taatcgtgaa ttctttcgta
     7321 gagaagaacc ggatggctta aatttggatt ttgattgttc cttttccaac ttatgtccaa
     7381 ctgctgcacc accgccaaaa gccagagttc ggatcacatt tcgattgagt tgccgcaatt
     7441 tacggttcat caacatgtgc ggtttccgca tgatgcgcct tcccattgac tgactatcat
     7501 tgctttggag attgaacata ctcatgatgt cacctaattt gaaatagata ccggcaaagg
     7561 tcacgatttg caggaacgca accataaaga aaggataggt agctgaaata ttgaaaaaca
     7621 tggtggagat actaaaagca gtcgtaatga ctaaggtaac tcccgctcgc atcataatcg
     7681 tattgaataa acgaataatc gccttctttc ccaagctctc ataagttggt aacatggata
     7741 acagaaaact aattggcaga aacatggcga aaatgataaa gagaatttgg gagaacaaca
     7801 taatccccgt cagtaagaag acaaagaatg agataccaat attaaacaac accaaaaaga
     7861 ccacggtccc tagtcggttc atggtctttg taatacttag attcgcgttg tcattgtctt
     7921 caatttcagc ttttaccgct tcttctcgat cctttccctt attttcatct ggactcacag
     7981 ataaaatttt attgacccgg tcttcaccaa tttcttctat gttggaatca tcaaattgca
     8041 acaagagcca aggttgtcgg acttgaatag aaaacaaact atcacgaatc aaatccacac
     8101 tgtcttttcc tttgctttcc gaattcggca caacgagctc tgttccgaga ctaagagccg
     8161 cttcactaac atccgaacta aagttattga tttttgtgat ataggtaggc gcatacgcga
     8221 taaaggaacc agagagaagg aagatcgtta caaagttaat gacagcccgg actgctttgg
     8281 tagcttctcg ctttaatagt ccgatatacg ccacatagac tcctagagct aaaatcatta
     8341 ataatagaaa tccaaaatag aaacccgaga cctgtaatcc attttcagta ataccggcta
     8401 gcgtttggat atttttccca atactttctg ccgtatccga aataaaatct aatttgtacg
     8461 cttcttgtac cacatacccg gtcgcatttg ataaatacaa actaacggtc caaatgaaat
     8521 ttgtaatggc gtagagaccg tacatcacac tctttcctaa cccatcgccc cagttccaag
     8581 gcagccagcc ccacgaacta tctacaaaga aatccaattg atagttatca agtgaatatt
     8641 gagaatagag attgccagat ttaacggtat catcaactaa tccagttgct tccgctactg
     8701 ttccaatcag actcaaaaga agtaaaatgg tgcagagact aattgcaatt attcccacaa
     8761 tccgtaaaag tttctttttc atctcctcac tccattctct tttgaacagg cgggcgtgta
     8821 tcaaaagcat ggaagagttc ttcaaagact ggatgcactt gtaccacacc aacgcgacca
     8881 tacaagtcct gcatcaaaca ttgcccattt tccaaatctc gcagacgctt ttgattgctt
     8941 tcatcttctt gatcaagacc aaagaattcg agtgtcttct tgatttcatg aatatctgtg
     9001 cttcgaaagg caaatttcag cccaatgttg tttttcagac gttcatctaa taaatcatca
     9061 ctattttgag tgacaaagta aacgccggca ttcatagcac ggccagcacg aactagttta
     9121 ttcgaaagag tttttccttg tgcgacttga aggaagctcc aggcttcatc caaatccacg
     9181 attttgaaaa tttctcgatt gctatgaata aaatccaagg caaatgtgga gataacaatc
     9241 aacatggaaa cactaagcaa ttccatcgta gtgtactctt caaatgtagt ctccgcatcc
     9301 ggcaaaacca aatctgccac ctgaatgata ttcaattgtt tctctaaact aatcgattgt
     9361 gtaattgaac catctgaaaa aagtaaatgc gcaaaatcat aatcggtaaa cgattcaata
     9421 tgatcagcaa tattttccgc aactggtgtc ccatcatttc gaagctcggt aatgacttgt
     9481 aacaacccac gttgattgct ttgggtaacg gttcgaatag cttttcgtaa aacgggaaat
     9541 ttctctccat cccgactaga aattccagtt agaaaagtca gaatatcaat tgctagactt
     9601 tcagaatcct tctttttctt catgatcaca tagggatcaa gtaacccttg attttctggt
     9661 ttactggtta ggttgaggat attaatttca ctcgcaattt ctggtaaggt ttctttccag
     9721 ctacc
//
LOCUS       DAFEZB010000086         9674 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFEZB010000086
VERSION     DAFEZB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.086"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(131..973)
                     /locus_tag="LP859_002415"
     CDS             complement(131..973)
                     /locus_tag="LP859_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3030317.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1010..2476)
                     /locus_tag="LP859_002416"
     CDS             complement(1010..2476)
                     /locus_tag="LP859_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3030318.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2532..4376)
                     /locus_tag="LP859_002417"
     CDS             complement(2532..4376)
                     /locus_tag="LP859_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3030319.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4978..5928
                     /locus_tag="LP859_002418"
     CDS             4978..5928
                     /locus_tag="LP859_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3030320.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5973..6606)
                     /locus_tag="LP859_002419"
                     /pseudo
     CDS             complement(5973..6606)
                     /locus_tag="LP859_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6866..7414)
                     /locus_tag="LP859_002420"
     CDS             complement(6866..7414)
                     /locus_tag="LP859_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030321.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7415..8272)
                     /locus_tag="LP859_002421"
     CDS             complement(7415..8272)
                     /locus_tag="LP859_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3030322.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8558..8845)
                     /locus_tag="LP859_002422"
     CDS             complement(8558..8845)
                     /locus_tag="LP859_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL3030323.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8835..9164)
                     /locus_tag="LP859_002423"
     CDS             complement(8835..9164)
                     /locus_tag="LP859_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3030324.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9241..9387)
                     /locus_tag="LP859_002424"
                     /pseudo
     CDS             complement(9241..9387)
                     /locus_tag="LP859_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(9439..>9674)
                     /locus_tag="LP859_002425"
     CDS             complement(9439..>9674)
                     /locus_tag="LP859_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3030325.1"
                     /translation="FYQGTEHRTIKYLNNLIEQDHRPVKRHNKFYRSLRTASTTIKGM
                     EAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIQA"
BASE COUNT         2907 a         1950 c         1491 g         3326 t
ORIGIN      
        1 agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata acaaacagct
       61 ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca tcaatagtat
      121 tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca cctaggtata
      181 caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct ttgatcttag
      241 ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat tcttcctcac
      301 catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca tgtgtaatga
      361 agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac cggaccaaat
      421 tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca ggatcttccg
      481 attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga agatcaattc
      541 catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct ttgggatagt
      601 agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt gcgcgttcaa
      661 ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt tttttatcta
      721 atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca ataggtattt
      781 cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga aatattttat
      841 ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc cctttaccta
      901 atgcaatcat ttctttgcca ttttcttcaa caagtaccac acttgaattc aatacttttt
      961 ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac tactcgaata
     1021 aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc tttttcaagc
     1081 ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca tatcgtttag
     1141 acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta taagctatca
     1201 aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta aagtagtcga
     1261 tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc ataccatttt
     1321 ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa attttcaaac
     1381 cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc ttaaccccta
     1441 aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc atggacatgt
     1501 aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga tcttcatctt
     1561 ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca ggatattctc
     1621 ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag gtcagctcaa
     1681 cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata caaccaactt
     1741 ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca acaaattgat
     1801 gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc tcttggataa
     1861 ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat gtcagccaat
     1921 attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca aaatcatcaa
     1981 ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc atttcataat
     2041 gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag acgttttcat
     2101 aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac aagcgagtcc
     2161 aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct aaatcttctt
     2221 tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca gatttgtctt
     2281 cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa tctttgttgt
     2341 ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg tctacattcc
     2401 aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct tttggaaaac
     2461 ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata ttttttttca
     2521 ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt ggttaccaat
     2581 ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac acagataact
     2641 gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag cttttgtcct
     2701 ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc taactgaaca
     2761 gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa accaatagca
     2821 tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa tatcccttct
     2881 tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt gtcttttact
     2941 tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc tgaaacaacg
     3001 gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata aagaaccaac
     3061 gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca aaccagattc
     3121 attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc cactaaagca
     3181 aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga aaacataacg
     3241 gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc agtaattccg
     3301 aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc ttttgttttg
     3361 atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga agccgggaga
     3421 taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat cattgcttta
     3481 tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt tgcaacccat
     3541 ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac attataacct
     3601 aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat cataggtaca
     3661 aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag ttgagcataa
     3721 aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt atccaatcca
     3781 aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag gatcaatgca
     3841 ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt agtcattgcc
     3901 accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat ctgataggtt
     3961 tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga cttcaatact
     4021 cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa atctaataga
     4081 actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac attgtcacct
     4141 aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat tacttggcat
     4201 tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct catatttacc
     4261 ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa atgggatatg
     4321 ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta attcatggtg
     4381 tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat cctcaacaca
     4441 aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga aacgcataca
     4501 ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg tttaagttat
     4561 attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt aatcattttt
     4621 tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt ttctaagaat
     4681 atcttccaac atatataact cttattctct tctatcttct agcagatgtt ctgtcttttt
     4741 aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga ggttttttat
     4801 ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta ctaaaaaatg
     4861 ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata tcgatattgc
     4921 ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt ttataagatg
     4981 aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat cagtaattta
     5041 gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg aagaacaaat
     5101 gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat caatactcgt
     5161 ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc ggtaaaagat
     5221 gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac agattccatt
     5281 ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca aatgaaaact
     5341 gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac tggagttgga
     5401 aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt tattcatgag
     5461 aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga attattgtcg
     5521 gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga tcgaaatgga
     5581 ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag attgcctcct
     5641 gaagggcaag agaaactatt tgtttatatg gatggcggaa cttattctag agtcggtgaa
     5701 actatccatc acgcctccaa tgtacgttta gtttttgcga ctacagaagg caaagaggat
     5761 tttctggaaa cctttatgcg aagaatccct atacatatcc atatcccaaa tattgaggaa
     5821 cgaggtattt tagaaaaaaa acaaattatc gaaaatactc tctttagcga aagcaaaaac
     5881 tttagcaagg ctattgatgt ttctcctaaa gtcactagtg ttgcataaat atagtcagaa
     5941 tattctgaat tattattttc ttgaaaaaag taaaaaaaat cgtatcccaa actttcatct
     6001 acatttccag aaagatcttc atgttgataa tcaattcctg aatcaatgac cgctaccttc
     6061 acatccttag aacccttggt ttcttcccaa gcggatggca tgctaatctc tcttaaatta
     6121 gcttgtttac tccaattagg atcgttatcg atttcctaaa gaatcagttg tatttggctc
     6181 ctcaacatca ttcaacggct ctagttcaac cacatagttt ggctctgcat aattgaccat
     6241 atcagaagca ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac
     6301 taataatact tgatctcgct tattataatc gctttcattt gtcctaagag tcaaatcttt
     6361 ttccaccata acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct
     6421 ttgtgtagcc tttaaactga caattagctc attgggtaca tactctattt gagagtcatc
     6481 taattggctc ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc
     6541 catgaaccca aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt
     6601 tttcatctac atctcctcct aatagtttta ttttagagtt agctctatta cttatttact
     6661 aactcaacaa cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca
     6721 ccctaaaatc ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa
     6781 aaataaatcc cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg
     6841 tagttactta gttcttcttt gattattatc taactttatt attcattccc caccctttgc
     6901 gcagtgagtc ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat
     6961 catctaattc tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt
     7021 cattaaacaa atcttgttta tcttcttgcg taagattctg tttaatatac tcatctaaag
     7081 cttttctaag caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg
     7141 aagtcgcatt taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa
     7201 actttctgat tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg
     7261 aggaagtttt tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa
     7321 ttgcttgctg ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt
     7381 tcccaaaaat atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct
     7441 cgtgttcttc caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt
     7501 gttttaagag acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta
     7561 agcgcgcatt tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt
     7621 gtttatacat ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag
     7681 caacatataa atttgtatca aattctttca caaaaaagtc catatactca agatattgca
     7741 tcgcatttct catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt
     7801 acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa
     7861 actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat
     7921 tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat
     7981 agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt
     8041 cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga
     8101 agttcatatg cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta
     8161 tcaaggttct ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac
     8221 ttttacctac gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac
     8281 ctcttttctt ttagcaatat tcatttgata ctattataac atatttattt ttagttacta
     8341 ccaaaaaaaa gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat
     8401 aaggaaaagc tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt
     8461 tatcgagaag cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa
     8521 aaaagaagcc gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt
     8581 cataaaagtt ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct
     8641 cgtaagctac tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata
     8701 aatccccagt tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat
     8761 tcaaaaattt ttctttcaat ggcttctctt ttagcttttt taaatactta atgacatgtt
     8821 ttgaaggaac gatttcataa gccaatttct gttgccaact cctctttagt cattacttta
     8881 gaattagtaa gtgtatcttc agcaatatct ctaaaggaag aatgcatttt gttttttcta
     8941 ttcttaaatt ctacaagaat ctcatcttta tcgtagcctt ctgcaattat atcagacaaa
     9001 atatcttcgc taaaatcata aaattctttt tgtggttcaa caaattcata aagaatacca
     9061 ttctctacca actttgcttc aacttcgact ccattaggaa cattaaaatc tttaggtact
     9121 gtcaacatga ttgaattgcc ttgttttctt gtttttgtaa tcatttaaat ccctcatttc
     9181 taacagtatg taattactta gtaaatacat tataccacga gataatgtat ttacaagtaa
     9241 ttactgtgac tcgtaatgcg tccttgctga ataaatttca accacgtgtt catcttcatc
     9301 taccttataa acaacccggt gttgtctatt caatcttcta aaatacctac cttcatgtgt
     9361 aggttttaac ttctcaaatg attgggttct gttgcaaagt tttaaataaa gaataaaatc
     9421 ccttacggta tctatgattt aagcttggat tcccaataat accttgattt cagtacagac
     9481 cgaaaacccg aagagagtgc cttcttttcg ggttttctta tataatcctc gaatggcttc
     9541 catgccttta atcgtggtag aggcagtgcg taaacttcga tagaatttat tgtgtctctt
     9601 tactggacga tggtcttgtt caatcaaatt attcaggtat ttaatggtac gatgttctgt
     9661 cccttgataa aagc
//
LOCUS       DAFEZB010000087         9665 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFEZB010000087
VERSION     DAFEZB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.087"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..650)
                     /locus_tag="LP859_002426"
     CDS             complement(<1..650)
                     /locus_tag="LP859_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3030326.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="LP859_002427"
     CDS             975..1391
                     /locus_tag="LP859_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL3030327.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="LP859_002428"
     CDS             1388..1858
                     /locus_tag="LP859_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3030328.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="LP859_002429"
     CDS             1872..2645
                     /locus_tag="LP859_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3030329.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="LP859_002430"
     CDS             2659..3471
                     /locus_tag="LP859_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3030330.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="LP859_002431"
     CDS             3525..4490
                     /locus_tag="LP859_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3030331.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTIVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="LP859_002432"
     CDS             4606..7329
                     /locus_tag="LP859_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3030332.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="LP859_002433"
     CDS             7342..7974
                     /locus_tag="LP859_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3030333.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="LP859_002434"
     CDS             8873..>9665
                     /locus_tag="LP859_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030334.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3261 a         1477 c         1884 g         3043 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactatcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAFEZB010000088         9317 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFEZB010000088
VERSION     DAFEZB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.088"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..2122
                     /locus_tag="LP859_002435"
     CDS             <1..2122
                     /locus_tag="LP859_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030335.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LP859_002436"
     CDS             2119..2739
                     /locus_tag="LP859_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030336.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LP859_002437"
     CDS             2853..2990
                     /locus_tag="LP859_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030337.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LP859_002438"
     CDS             3432..3812
                     /locus_tag="LP859_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL3030338.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="LP859_002439"
     CDS             3794..4708
                     /locus_tag="LP859_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL3030339.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LP859_002440"
     CDS             4948..5637
                     /locus_tag="LP859_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030340.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="LP859_002441"
     CDS             complement(5758..5844)
                     /locus_tag="LP859_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL3030341.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="LP859_002442"
     CDS             6489..7226
                     /locus_tag="LP859_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3030342.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="LP859_002443"
     CDS             7254..7793
                     /locus_tag="LP859_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL3030343.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7809..7979
                     /locus_tag="LP859_002444"
     CDS             7809..7979
                     /locus_tag="LP859_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030344.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8291..8752)
                     /locus_tag="LP859_002445"
     CDS             complement(8291..8752)
                     /locus_tag="LP859_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3030345.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="LP859_002446"
     CDS             8885..9259
                     /locus_tag="LP859_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBL3030346.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3597 a         1414 c         1454 g         2852 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt atcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt
     8101 aaaaaaatga cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag
     8161 ctttagaaag ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     8221 ttggtagagg ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaataactag ggggaaaaac attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccatg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DAFEZB010000089         9248 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFEZB010000089
VERSION     DAFEZB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.089"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            154..1443
                     /locus_tag="LP859_002447"
     CDS             154..1443
                     /locus_tag="LP859_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL3030347.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            1456..4158
                     /locus_tag="LP859_002448"
     CDS             1456..4158
                     /locus_tag="LP859_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL3030348.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            4143..5000
                     /locus_tag="LP859_002449"
     CDS             4143..5000
                     /locus_tag="LP859_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3030349.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(5071..5970)
                     /locus_tag="LP859_002450"
     CDS             complement(5071..5970)
                     /locus_tag="LP859_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL3030350.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            6139..8310
                     /locus_tag="LP859_002451"
     CDS             6139..8310
                     /locus_tag="LP859_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL3030351.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            8378..8719
                     /locus_tag="LP859_002452"
     CDS             8378..8719
                     /locus_tag="LP859_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL3030352.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(8995..9225)
                     /locus_tag="LP859_002453"
     CDS             complement(8995..9225)
                     /locus_tag="LP859_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030353.1"
                     /translation="MLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEAD
                     KIVVIDEGKIVEIGTYEKLLAQKGYFYRLKNE"
BASE COUNT         3153 a         1647 c         1990 g         2458 t
ORIGIN      
        1 tcatataaac acctcttaat gatatatcat attcaaataa taccataatg gatgaaaaag
       61 taaataaaaa gatatttttt gaaagcgtag acataattgg ctgtatgaag tataatcttt
      121 aaagatatat caaatagaaa gaaggtttta ttagtggctt ataaagatat accaaaatcg
      181 gtgcagaaat ttatggaaga tatcacacaa aaatgtgggg atatccatgc ggactgggca
      241 aagaatttta cagcagcatt tgccaatact ctgttgacaa cagtcaaaaa gcaaaatgac
      301 ggaaccacct ttttattgac gggagacatt ccagcgatgt ggttaagaga ctctactgca
      361 caagttcgtc cttatttagt gattgcaaaa gaagatgagg aattagctga catgatcagc
      421 gggttagtca aaaagcaatt tttttatatc aatatcgatc catatgcgaa tgccttcaac
      481 gaagaagcta acggtgctgg ccaccaaaca gatcatacaa aaatgaacga ttggatctgg
      541 gaaagaaaat atgaaattga ttctttatgc tacccaatac agttggccta tcttctttac
      601 aaaaatacag gaaaaactga tcaattcaat caatctttta ttgaaggtat caaaaaaatc
      661 ttggacgtgt tcaaaacaga gcaagatcat gaacactctc cttatacatt tgtacgagac
      721 acgacaagaa aagaagatac gctgatcaat gacgggcgag gtgcaaaaac agcttttaca
      781 ggaatgacat ggtctggttt ccgtcctagc gatgatactt gccgttatgg atatctggta
      841 ccatccaata tgtttgcggt ggtcgtgtta ggttatgtgg aagaaatttt tcaaacgata
      901 ctcgatgata aagaaattgt acagcaaaca cagaaattga agaaagagat ccaagagggg
      961 attcagaatc acggacgaac ggttaatcaa aaagggcaaa cgatttatgc atatgaagta
     1021 gatggaatgg gaaacgccac agtcatggat gacagtaatg tgcccaatct gatcgccgca
     1081 ccttacttgg gatattgttc tgaacaagag gaagattatc tgcaaacaag aagaacattg
     1141 ttgagtaaag aaaatccata tttctatgaa ggaaagtacg caaaaggaat tggatcctcc
     1201 catacgccag aaaaatacgt ttggccgatt gcattagcta tggaaggaat gacaacgtca
     1261 gacaaatctg aaaaagagcg gatattgaat ttacttgtag caactgacgc aggaacacac
     1321 cttatgcacg aaggattcga tgtgaacaat cctgaaaatt atacgagaga atggttttct
     1381 tgggcaaata tgatgttttg cgaattagtc atggattact ttgatattag agtagaaaag
     1441 taggaggtat cgtaaatgaa aaagaaagta tatatcattt ctcatagcca ctgggatcgt
     1501 gaatggtaca tggcttacga acagcatcac atgcgactga ttgaattgat agacgatcta
     1561 ctagagctat ttgaagttga tccgtccttc aacagttttc atttggatgg gcaaacgatt
     1621 attttagatg actatcttca agtaaggcca gaaaaaagac aggcaatcca acaagcaatc
     1681 aatgaaggaa aattgagaat tggaccattt tatatcttgc aagatgattt tcttatcagc
     1741 tcggaatcga acgtgagaaa tatgctgatt ggcatggaag aaagccgtaa atggggaacc
     1801 cctgtcatgt taggctattt tcccgataca tttggaaata tgggacagac gcctcaatta
     1861 atgaaacaag ctggtatttc agcagcagcg tttggccgtg gtgtaaaacc aattggcttt
     1921 gataatcaag ttttggaagc agaaaactat tcttcccaat actcagagat gtggtggaaa
     1981 ggacctgatc aaacagcaat ctttggtctg ctatttgcca attggtatag caacgggaat
     2041 gagattccag tagaaaaaga agcagctctt gccttttgga agcaaaaatt agcggatgca
     2101 gaacaatatg cctctacgaa ccacttattg atgatgaatg gtgttgatca tcaaccggta
     2161 caaaaagata tatcaaaagc aatccatctc gcaaatgaac tgtttccaga ttacgagttt
     2221 attcattcta attttactga ctatctggaa gcagttcaaa aagatgtgcc agaagactta
     2281 ggctcagtag aaggagaatt gacaagtcaa gaaacggatg gctggtatac tttagccaat
     2341 acagcatctg ctcgggttta cttaaaacaa tggaatacca aggtccagcg acagctagaa
     2401 aatattacag aaccacttgc gacgatggct tatgaagtta gtggaaacta tccccatgat
     2461 caattagatt atgcttggaa gacattaatg caaaatcatc ctcacgacag tatttgcggt
     2521 tgttcagtcg attctgtcca tcgggaaatg atacctcgtt ttgaaaaagc agatgaagtt
     2581 ggaaagtatc ttgcacaaga ttccttagaa cagctgaccg cagcaatcga tacgaccgga
     2641 ttcccaaaag acagctttcc atttgtcatt gtaaatactg caggaatgga taaaactggt
     2701 gaagcagaaa taaccatcga attagaaaga aaaagattcg ctgaaggaat acctgaacaa
     2761 ctatatcaag agttagaaaa tttgcctaaa cgtaagtatc atgtagaaac aaaaagtggg
     2821 gccactatac cagctatttt atctgaagaa actgtccaat ttggttatga tttgccgaaa
     2881 gaccgtttcc gtgtgccata tatggcaaga atgatcaaag tgactttacc attagaaaac
     2941 atgccagcat tttcttggga aacattcgcg cttgtagaag gggaagcgaa agcagaagaa
     3001 aaggaaacaa tgattcatca gagtggacga atcatcgaaa acggccctct acatttgacg
     3061 attgaaaaaa atggtaccat tacaatggaa gatcgcaaaa acaaacgaaa gttgaataat
     3121 ctgcatattt ttgaagatat cggcgatata ggaaatgagt atatctttaa acaacctttc
     3181 tgtgatcagc caatcctatc cagtaacaaa gaaaactctg aagtaaaagt attagtagat
     3241 acaccggaaa tagcaaaaat aagtattcta caggaaatgg aaatccctgt ttctgcagac
     3301 gagcgtttag aaaaagaaca gcagatggtt gtggaatttc gttatcgaaa agcagaacga
     3361 tccaaagaaa aacgaatcct tcaaataaaa acaatcatga caatcagaaa agatagtaaa
     3421 aaaatcgact ttgaaacgac tctcgacaat cagatgaaag atcatcgctt acgtgtcttg
     3481 tttcctacaa agttacatgt ggaacatcac gaagcagata gtatttttga agtggtgaag
     3541 cgcccaaacc atgtgtctaa aagttgggaa aaccccacga atccgcaaca tcagcaagca
     3601 tttgtcaata ttcatgatga agagtacggt gtaacagtcg gaaactttgg tttgaatgaa
     3661 tatgaagtaa cagaagacgg acaaatcgca gtgacgctac ttcgcagtgt gggagaacta
     3721 ggcgattggg gatattttcc aacaccagaa gcacagtgtc taggtgagca tcggttcaat
     3781 tacagcattg aactacatgg accagaagaa aaattctcta cttatttgca tgcatacgca
     3841 gcacagattc ctttttccac tcaacaaatc aaacatcatg aaggaacatt gatttcaaaa
     3901 caacaatatt taacgataaa aagtgaaacg tttgcaatta cagctttgaa acgcagcaag
     3961 tttagtgata aagtagtagt aagaggtttc aatatgtcta gtcatttaga aaaattggaa
     4021 ataactaaag acaatggaaa aactgtcata ttgaatctgc ttgaagaacc aacgaagcaa
     4081 gcggttgtcc caataatcca accttatgag attcggacaa tcggattcga ggaggaaaac
     4141 gaatgtatgg cggaatagaa gcaggcggaa caaaatttgt gtgtgcggtt tcagatggtg
     4201 gagaaatttt agaaaaatta agtatcccta ccacaacacc agaagaaaca ttggatcagg
     4261 tatttgaatt ttttgatcgt tatgaattga agggaatcgg gattggctca ttcggcccga
     4321 ttggtatcga caaaacaaaa gataattacg gatacgtttt ggcaactccc aaaaaagggt
     4381 gggcacaatt cgattttctt ggcacaatca aggaacgcta caatattcct gctgcttgga
     4441 caacggatgt caatgctgct gcatatgggg aattgaagca aggagcagca caaggaaaaa
     4501 atagttgtat ttaccttacc gtgggaacag gaatcggtgc aggtgtcgtg atcaatgaag
     4561 aaattttttc agggatcgca catcctgaaa tgggacatat ttgggtgaaa cgccatccag
     4621 atgaccatta tgaaggtact tgtccttatc ataaagattg tttagaagga ttagcagcag
     4681 gaccgtctat tgaggcacgc acagggatca aagggcaaga tctgccgcaa gatcatcctg
     4741 tttgggctat tcaggcttat tatattgctc aagcactgat caactatacg ttgacgttag
     4801 cacctgaaaa gattatttta ggcggtgggg taatgaatca ggatcatctc cttcaaaaaa
     4861 tccgtcagca atttgttgaa ttgatgggtg gttatatgga aacacctcaa gtaagtgaat
     4921 atatcgttcg ttggggactg ccgaatgaaa gtgggattat cggtagtttg ctgttagcag
     4981 aacacgaaca tcaaaagtaa gcagacaaat tacacaaaaa gaacagagtc ttatcattat
     5041 ctgctgataa gactctgttc ttttttattt ctattcccat aatccaggta aagctttacc
     5101 gactattttt tcttgagtaa atggatggat catgtgcagt tctaatgcat ggagcatcag
     5161 tcgttcagct gttctatttt ggtagagagg gtctccaacg atcggatgtc ccttatgagc
     5221 taaatgaact ctgatctggt gtgtacgtcc ggtttccaaa acacaataga cttgtgtttg
     5281 ttcagaaagt actttgttga cagtgacatg tgtcaaggcg ctctgaccat ttttatcatc
     5341 tacgaccctt tttctccgat catggcgatc tcgtccaatt ttattggtga tggtcatatc
     5401 tttggcaatt tctccccata cgatggcttg atatctccga tagatcattt tttgttccaa
     5461 cattcttcca agtatcggca agacaaatgg attttttgca aataaaacgg caccgcttgt
     5521 ttctttgtcc aagcggtgga caacgtaagg tcgttgctgt ttagtgtcga gataagcagc
     5581 taaatgattg agtaaggtac tgttttctgt cggttcattt ggatgagtct tcattcctgc
     5641 aggcttatcc actataatca aatgctcatc ttcatataaa acacggatgt gcttcgcatc
     5701 tcctaattga acttcttgat aattataatc tgtttcttcg aataacaatg tgatctggtc
     5761 attgcccttt acttcatgat ggaacaaagc aggttgttga ttgatccaaa cgtttttacg
     5821 tatacgcaaa aagtgtcgaa cctttctagg aactaaccat tccttttcaa gcaagtcgcg
     5881 aattgttgtt tcaggatggt tcataggtaa agtgatcgtg attttcacaa gtgtactcct
     5941 ttcagtgcaa tcgcagacta attgtaccat ttttaaaaca tatttaagaa aaaacttaca
     6001 tttttctgtt cattcttatg ttactctact tgcactaacg taaaaaaaca tattgaacta
     6061 caacttaagc aggaagaaaa cactctttca ctatggtaaa ataagacgat tgaatcaaat
     6121 agacaggaga agaagcaaat ggattttcaa agcattttag aaaagataaa agctgctttg
     6181 atcagttttt ggcgttggat cagaccatat ctcggacaat tccatcgatg gcgaaagcgc
     6241 atctggaaaa aatatcatgt gaacaaattg attttgctga ttggtttgat ctgtgtctta
     6301 gtgacaagta tctacctctt tattttagca aaacaagcaa atgtcgaaac gttaaaatca
     6361 ggattaagcc aatcgacagt catttatgat aagaaaaacg aagaagctgg cacactgtac
     6421 gcccaaaaag gaacatatgt agaattaaac gcgatttctc cctatatcca agatgcagta
     6481 atctcgacag aagataggaa tttttacgag catcacgggt ttgatatcaa agggattgcc
     6541 cgtgcagcag tccgaatggt cttgtctggc ggaacatcag ggggcggagg aagtacgatc
     6601 actcagcagc ttgccaaaaa tgcgtatctg acattagacc aaacgtttga tcgaaaagcg
     6661 aaggaattat ttttggcaat cgagatcgaa aagaagtaca gcaaagaaga aatcttgacc
     6721 atgtatctta acaatgccta ttttggcaac ggggtatggg gtgtccaaga cgcttccaga
     6781 aagtattttg gagtagatgc aacgaatgtt acgctgtctg aggctgctac tttggcagga
     6841 atgctgaaag gtcctggtat ttataatccg atcgatcacc caaaaaatgc gaatgatcgg
     6901 aaaaacacag tactaagtgt aatggaagaa aacggaaaaa tcacaaaaga acaagaacaa
     6961 gcagaaagcc agacagatat cagcgcgtat cttaatgaca catacgataa ttcagattct
     7021 ggttatcgtt atccttatta ttttgatgca gtgatcgatg aagcagttaa cgagtatggt
     7081 cttgatgaag aagatgtcat gaataaaggc tataaaatct atacagcttt agatcaggat
     7141 tatcaaaagc aattagaagc tacttatcaa aacagtgcat tattcccagc aaatgcttca
     7201 gatggtgcca tggtccaatc tgcttcagta gctttagatc cgaatacagg tggtgtacaa
     7261 gcactagtag gacgaagagg agatcatgtc tttagaggat tcagctttgc tacacagatg
     7321 cgtcgttcac caggatcaac gatcaaacca ttaagcgtat atacaccagc actggaagct
     7381 ggatacaagc cttccagtat cttgaaagac gaaccgcaaa gttattacga tgcgcataat
     7441 tttgacggga cataccaagg agaagtaccg atgtatcaag ctgttgcaca aagtttgaat
     7501 ttgcctactg tatggctttt gcatgagatc ggtttacaaa aaggatatga taaagcagaa
     7561 gaattcggcc tgcctttagc aaaatctgat aagtattatg gcttagccct tggtggactg
     7621 gaaaaaggag tatctccatt gatcatggcg ggtgcttaca gtgcatttgc caatgatggt
     7681 cagatgtata cgccgcatct tatcacgaaa atcgttgatt cgactggagc ggtgatcgtc
     7741 gataacacaa acaaaaaacc aaaacaggtc atatcaaagg aaacagccga tgaaatgacc
     7801 agcatgcttc ttggtacatt ttcaaatgga acagcggctg ctgcgaatcc tgcaggttat
     7861 acgatagcag ggaaaacagg aaccacagaa actaatttcg atgccacaaa agcaaatgac
     7921 caatggatga tcggttacac gccagacgta gtgatttcga catggatggg ttacgaagaa
     7981 tccagcaagc tgcactattt ggaagggaca agtggaaatg ttgtcgggaa agtcttcaaa
     8041 tcagcggcag aaggcatcct tccgtatact tcaaaaacag gatttagtgt agcagatgcc
     8101 tatgccacag gaggacaagt ggtagctgca gatcaagttg gaaacacagg aaattctaat
     8161 ggagagtctg gaaattggca agataacttg aatcaatatg gtgaacaagc aaaagaaggg
     8221 ctgaaaaact tcggcgatat ggtgaaagaa ggcgttcagg gaattggtga agcggctaaa
     8281 gacctctggc ggaaatatca aggagagtag cccctttgaa aacgaataac cccatgttac
     8341 aatagagagt aactactaaa agaaaagagg gcataacatg tcaaacatct acgatagcgc
     8401 gaatcaaatc gaacgtgaga tccgtgaatt accagaattc aaagcgttga aagaagcgta
     8461 cgagaaagta caagcaaacg aagaagcaca caaactgttc aaagacttcc aagcgatgca
     8521 aatcgaatta caagaaaaac aaatgagtgg acaagagttc agcgacgaag atgcaatgaa
     8581 agcacaagaa atggctgtga aaatccaatc agaagaagta atcaatgact tgatgcaaaa
     8641 agagcaaggc ttcagtacaa tcatcaatga cttgaaccgt atcatcatga caccagttcg
     8701 cgacctttac agcgaataaa actattttat gaaacaacag agggtgtgaa agagacgttc
     8761 ggccttgagc gatgacaaaa ttgcttctta ttgccgatag gctgtctctt gaacaccatt
     8821 taccaagagg tcgtgagcct accgtttaac tctgagttac aaggaacaat ttcaaaaaac
     8881 agcttctcat gtttgatgac tattgtgact tgtgaccgag gagttggcag gagaacaccg
     8941 tttattcaga aataggagct acgacaaaag tcttgtcata gctcctattt tttgtcattc
     9001 atttttcaag cggtagaaat agcctttttg agctaacagt ttttcgtaag tacctatttc
     9061 aacgattttt ccttcatcga tcacaacgat tttatctgcc tctttgatcg ttgaaagccg
     9121 atgagccaca ataaaagtag tgggtgtttc tgtcaggtaa ttcagggctt gctggatttt
     9181 tttctctgac tggttatcta aagctgaggt tgcttcatct agcaaaatga tttttggctg
     9241 acgtatca
//
LOCUS       DAFEZB010000090         8966 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFEZB010000090
VERSION     DAFEZB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.090"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(54..347)
                     /locus_tag="LP859_002454"
     CDS             complement(54..347)
                     /locus_tag="LP859_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL3030354.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(375..1040)
                     /locus_tag="LP859_002455"
     CDS             complement(375..1040)
                     /locus_tag="LP859_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL3030355.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1180..1812)
                     /locus_tag="LP859_002456"
     CDS             complement(1180..1812)
                     /locus_tag="LP859_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3030356.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1819..2886)
                     /locus_tag="LP859_002457"
     CDS             complement(1819..2886)
                     /locus_tag="LP859_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3030357.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2883..3614)
                     /locus_tag="LP859_002458"
     CDS             complement(2883..3614)
                     /locus_tag="LP859_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL3030358.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3783..4262)
                     /gene="greA"
                     /locus_tag="LP859_002459"
     CDS             complement(3783..4262)
                     /gene="greA"
                     /locus_tag="LP859_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL3030359.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4398..5573)
                     /gene="mltG"
                     /locus_tag="LP859_002460"
     CDS             complement(4398..5573)
                     /gene="mltG"
                     /locus_tag="LP859_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL3030360.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5777..6946
                     /locus_tag="LP859_002461"
     CDS             5777..6946
                     /locus_tag="LP859_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL3030361.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7213..8847)
                     /locus_tag="LP859_002462"
     CDS             complement(7213..8847)
                     /locus_tag="LP859_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030362.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2854 a         1701 c         1333 g         3078 t
ORIGIN      
        1 attttcaaaa gaaaaaagag aaggtgacaa aaattgtgtc accctctctt tttttattgt
       61 tttttaaagt gataggttgg ctcctccagg ttgggatcat aatcaacaag taaatcatat
      121 cctttaaaga accattcatc tgcttcctcg ataaaaaaaa gtgtcccatc aatttccact
      181 ttctttacag gatgttctgg ctgttcaact gacattccaa tcgaaaaacc atcatgaacc
      241 tctgttttgc cgtagacttt tccaaaaaaa cgtattccgt aagtttctgg cagatcaatt
      301 tctgagtcaa accacttttg tgcttttggc gttaccgtta atttcatccc aacaacctac
      361 tttctctttt ctttctattg attcatctta tcgtctagaa tatccacatc gcctgtctct
      421 ccaataacca gtagattatc attttcacga acatattcat ctgctgcagg agaagcgata
      481 accgtttgat ctactcttcg tatggcgact actgttaagt caaaacgttg cctgaaattt
      541 aattcagcta atgttttgtt gaaaaattta ggattggaga ctcggatttc tgctaaagaa
      601 tatttatctg acaactctag gtaatctaaa atattctttg acactaaatt atgcgcaatc
      661 cttattccca tgtctcgctc gggatggacg acgcgatcag ctccgatctt ttcaagtact
      721 ctggcatgat actcattttg ggcttttgct aaaatatttg gtacgcccat ttcctttacc
      781 atcagagtaa ctaagatact ggcttgaata tcttctccaa tagcgacaat tacatgatcg
      841 aaatttctta tgcctaatga tcgaagtgtc atttcatcct gtgcatttgc cacaacagca
      901 tgagtggcta tattcatata ttcattgacc cgatcttcat tactatcaat agccagtact
      961 tcttgaccag cttcgatcag tgttcggcag atacttccgc caaatcgtcc aagacctaca
     1021 attgcaaaat tttgtttcac cgttttcctc ctttagtatc ttcttttctc agtataacaa
     1081 agcctttaca aaaacaaaag caagcatcat tattgtaaaa aagccaagaa aaataatagt
     1141 tattttctca gcttttgatt atcctcttta tcatcgtttt tatttcacca aaccatgttt
     1201 gaatgcatag atagcggctt gtgtacggtc ttccacttct agcttagcca atatattgga
     1261 aacatgtgtt tttactgttt tcagtgtaat aaacaattcg tccgctattt cctgattact
     1321 ttttccttca gaaatcagca ttaaaatttc attttcccga ttcgttagtt cttcatgtaa
     1381 tacaggctca tttcgccgac tcattttttc catcattttt gttgtcactt ctggttctaa
     1441 tactcgctcg ccacgttgcg ttgcccttat agcatcagca atttcatgtg cggtagaggt
     1501 cttcaacaaa taccctgctg cacctgcttc aatggcagga taaacttttt cgtcatcaat
     1561 aaaactggtt acgataatga ttttagcttc tggccaatct tttaagattg cttttgttga
     1621 ttcgatacca tccatttctt ccatgactaa gtccattaaa atgacgtccg gtcgaagtgt
     1681 catcgccttc tcatagcctt gtcgtccatt ttcagcttct ccaattactt caatgtcttc
     1741 ttgaatagat aaataagaag agacgcctaa gcgcaccatt tcatggtcat ctactaataa
     1801 aacttttatc atccttatct atgcctcctt catcaaaggg accttgattt ctacacttgt
     1861 accttgacct ttaaaactaa tgatttttac tgttccacca ataccagcta ctcgttcttt
     1921 gatattgttc aatccataac ttcccgtttt cgtttcattc atattaaacc ctgttccgtc
     1981 gtcaataata cgtaacaaaa gattattgtc tattttgtgc aaatatacct ctaattcatt
     2041 ggctttagca tgtcttaatg tatttgataa caattcttga acgattcgga acaagtgatc
     2101 ctcaatggaa ctagatagtt ttacatcttc tacatcccat ttcagcgaaa tctggatttt
     2161 attttgcaat tccttcaata actgctcgat accttgcttt aaacttttct cttctaaatt
     2221 gactggacgt agatgaagaa gcagcgcacg catctcagac tgggaagcgt tgatgatctc
     2281 tgctaccatt ttcaactgct tgcgaaacat ttctggtgtc tcgctttttt ctgcttgctc
     2341 cgtcaatgcc gacatcatca tcattgctgc aaacaattgt tgcgaaactg aatcatgcag
     2401 ctcacgggct aatcgatgtc tttctaattc taaaatttct tctttggttt gtccatctac
     2461 atattgggga cggctcgtta caatttgtaa ttcactagat acttcaatca ttttttcttt
     2521 tatcttagtg atttccttat caatatcttt gatgtataaa tcgtccgatg cacttgggat
     2581 cggaagaagc aacaatttgg attcataatt gccagaagaa agtgcacgca gcttttcttc
     2641 gattttcccg tattttgtct tttgaataag tgataataat agaaagacaa ttaatcctac
     2701 acctaaggaa atggctaaca aatggaagat aagcggaaca tataataaac gtgcacgcag
     2761 taattctccc caccaatgac tttgattgct ggcataaaaa taagtgaaaa gtgaaaatag
     2821 aataataagg aaagccgcaa tccccgaata aacagctagc atcgctctgg atatttttcc
     2881 catcatattc gtatcacctc gacatctcca atcaaggtat tagtaataat cttcaatcta
     2941 cgagggtttt catcataatc actgctgtag attttgattt gttcattttt caaagaaaat
     3001 tgctcttctt caaacaagac atttcctact aatgtcgcat gctctaatcg gatggctacg
     3061 cccaaaggta ctaagatccg tgttctgcct atcccttttc ttacaatcac aatattatca
     3121 tcttttggca aaagcgtatt gcccaaatct atgatagtat caccagaaat gacggcgatg
     3181 ttgatatcgt cccattcata gacgtcgttt ccaatacgtt ggttaccgaa taattgctgt
     3241 cttttccgct cattattatg tgtttttaat tgctctgttt ggatcatcat gatctgtttc
     3301 tttcgccaaa aagcattttt ggttaaatct atccctgaaa tctctacacc tttcagacca
     3361 ataaataaaa cggcaaatac agccatcatc cataaagcag gactgttgac taagctaaaa
     3421 aaaatgacca aacttcctaa tatcaattga aaattttgaa atttagtacg aggatgtttg
     3481 cgcatagcta gatatatatt gaaaataccg aatatcacta aaagcaaaag tccagtattg
     3541 tttacgattt gccaaacagc aaataacagc agtagtgctt caactaccac aaaaaaacgc
     3601 caagaactat tcatcgctct ttctccctcc tgacacattc tcatttcatc atacctattt
     3661 ttttaactgt tggctatagg actttagatg taagatcgtt tgttgaaatc taaaatacac
     3721 aaagagaaaa agatctagag tgtatcatca gtaagatgaa ctctctagat ctttcagtca
     3781 gactacgctt gttctacttt gacgatttta actaacatat cgccacctgg agtagcaata
     3841 gttacttcat catttttacg tttgccaatc aatgcttgcg caataggaga atcattggaa
     3901 attttgccag aaaacggatc tgcttcagcg cttccgacaa tcgtgtactc ttcttcttct
     3961 ccatctggta gttcgatgaa tgtaactgtt ttaccaattg atacctcatc tgcatctaca
     4021 ccgtcgttat cgatgatttg agcaaaacga atcatatttt caattgttgt gattcttcct
     4081 tcaacaaatg cttgttcatc tttcgctgat tcatattcag agttttcgga aagatctccg
     4141 aaaccgcggg caattttgat tcgttcaatg atttctccgc gtttgactgt ttttagttct
     4201 tctaattctt tttctaattt ttctttacct tctagagtca ttggataaac tttctctacc
     4261 atgtgtaacg tctccttttt tcgataaaat accgcaaaag gtaatctttt gaggaatata
     4321 gtccaaaaaa gattaccacg cttttcttca aatgtaaatg tttttctaaa aatgatcaat
     4381 gtctagagcc tgtttttcta agaattattt acatattttt ccactaattc catatgttct
     4441 tcataagttt tagcgaaata aacatttcca gtgctcgtat ctgctacgaa ataataatag
     4501 tcattttctg ctggttctaa tactgccttg attgcctctt cgcttggatt gtcaaaaggt
     4561 ccaggtccat agccggtatt ggtatacaaa ttatatggtg aatctacagc tgtatcttca
     4621 tacgtcacta attctttgtg ttcacctaat gcgtataaaa cagagatatc tgactgaagt
     4681 ggcatatttg ctttgattcg gttaaagaag acttgtgcaa tgtttttacg atcattttct
     4741 tttacgcctt ctttttcaac tagagaagct agagttaaaa cttcttgtac agtcaaattt
     4801 ttctgcttga tagctgaata atagttttgc atgaccgtat tcgtcttgtt caccatttca
     4861 atcactagat ccttaagcga agtactcgtg taataatcat atgtggctgg gaataagtaa
     4921 ccttccaaac ggtattttgt gttctcagct ttagatgcac tttccaacag ttctgggaat
     4981 ttttggtgca actcgttaaa gaaagtctca tcattcacta aatctaaaaa ttctttcttg
     5041 tctttaccag ttaccttcgc tacccgttca gcaatctgat cgatatcgta cccttctgga
     5101 atggcaattt tcccatctgc aacttttgtt ggctcagaag taccgccttc ttgcaactgt
     5161 ttgccaatct cgtcaagagt catattaggc gctaactgat aatagccagc ttgaaagcca
     5221 gttaaatttt taaattttgt ataataattg aaaacaatcc cgcttttaat aatattgtcc
     5281 ttctccaaaa tctcaccaat ctgcttgtta gaagagccac tcgggatctc gacttggact
     5341 aattggtcat ctgatttatc taaaggcttc aacccgctat ctacataacg atacacggta
     5401 aatccaagaa atccgccaat aatcaataat gtaagagcaa tcactaaaat gatcttcctc
     5461 acaatcttat cttctttttt acgcagattt ctccgcttgt cgattatatc tgctgtagct
     5521 gccttactgg cttctttttt atctttactt gggtgttgtt tgtttcctga caaaacaact
     5581 cctccttact atcaagttcg taactttatt cattatacat gatatttgaa ttggcgtgaa
     5641 tgattttatt taaatattct atcaaaaatg taagtagtaa atccattact ataagattaa
     5701 aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa aaagaaagga
     5761 gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat attcattttt
     5821 aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta ttcttggatg
     5881 gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag ctcaaaaaaa
     5941 gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac tattaagaaa
     6001 aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg
     6061 ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt attatcagat
     6121 tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg agcgtgggca
     6181 cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca gccagcaaat
     6241 tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt ctccaaccat
     6301 accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat ttacagatta
     6361 tcagacctct cattatgatt cattgaactt taccatattg actggaattt tgatcaaatt
     6421 aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt taggattaca
     6481 gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat cctatcaaat
     6541 gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga tccataacga
     6601 attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt ttacacaagt
     6661 tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg aaaatgcgaa
     6721 tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt cgcaaggaaa
     6781 tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg ctatcatcat
     6841 ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc taagaaacca
     6901 aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg atgaaaagat
     6961 agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact aaacgtagga
     7021 aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc caaataaaca
     7081 gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat ttctctttac
     7141 caacattatt tgacaaaggg cctattaaat ctaaaatagt atctttagat tgctaaaata
     7201 tgtgctagca aatcattcat ggcttgttct tatttttaat tttatgcaat aaaaaagaag
     7261 aacaattagc gtgattatag aaataaataa tgagatttta gaaaaaaatg tgtatgaata
     7321 cctgattgtt actttattta ttccactttc actttttact gtaacaaaag gacctccttg
     7381 ttttagtact gtagcatttt ttgaattaac agtaaccgtt aatcctttat accggaatac
     7441 aggtaaaact attgttttgc cttttgaaga tttaagcgta aatgtaattt catctttatt
     7501 tggagaaaag ctactataaa taacttgacc atcttctgta acttgatggt ttttcaaaag
     7561 caattgatct tttttatagc ctataggtac ataatcaaaa ttctcaaatt cttcaagttt
     7621 attttccatt tgttcattag aataagttcc ttgaaaattc atttctttca ccatgttaac
     7681 tactgatgaa taatttaaaa ttgaaatagc taatattaat aatagtataa aatttcttct
     7741 ccttgtataa gaaaattttt ccaactgtgg agaaataaat agagatactg aaaaagatat
     7801 gaacaatgtt gaaaatatca taaaacgcca aggagcttga ataatattta aaggagtatt
     7861 ttgtaaggca aaccatggaa atatttttgt acaaagtaga atagatatta tacccattat
     7921 ataagtgatt ttagataaga ttcctaattt tttaaagtta aatgttagta taattaaaca
     7981 aataagcaaa gttaagccaa taccaaatga tagtaattct gaatctatac ttccaatcaa
     8041 aaactcttta cccagataaa ttgctctttt ttgtaatata tccacataag gtctattcag
     8101 atcttgatac aatgtctgct caatcatagg acctataaac gctagtgaaa gaagtaaagt
     8161 aactatagca gcttttatta atttaataat taagaagatt tctattttct ttttccgata
     8221 aaattgaata attaaagcaa ttataatcat taccatagtc atagctacag aaagaacatg
     8281 agtgtaaatt aataaagtca tgctaatcgc caacataacc caagattctt tttttgagtc
     8341 gtaaagcttt ataaacccga gtaaaataag aggaagtatt gacattgcga gtaattcccc
     8401 cattgcccct cgaagaaata tttctacact cctaaaagaa gatgttgtat atataatagc
     8461 gaaaaaggag ctagtaacat gttttttggt aatttcatac attacataat gacatataaa
     8521 tagagtaatg agcgtaacaa tataaagaaa aaggttatag cctattgcta aattagtaaa
     8581 cttgatcaat aaaaataaag gatatagagt taaccatggg taaaaattat ttacttgcat
     8641 gccatatgat ctaaacgttt caaaattaat aggggagcta aaaatagatt ctaaacttaa
     8701 cgcacgatta atgtgaaatg ccgtatcata aaagccaaat atttccatgt ttttaaatgc
     8761 tggaaataaa tataataaac taacaaatgc ataaactata aatattttta cagaaaaaat
     8821 ttttttcaaa ttcatatacc acctcatgtt tttttatatt acatatagta tcatttattt
     8881 ttttaaaaca ctagattagt cactatataa aatgtgatta attttattat taataaaata
     8941 ttttttatat tacaaaagta gtgttt
//
LOCUS       DAFEZB010000091         8603 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFEZB010000091
VERSION     DAFEZB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.091"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP859_002463"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP859_002463"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL3030363.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LP859_002464"
     CDS             complement(1098..2201)
                     /locus_tag="LP859_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL3030364.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LP859_002465"
     CDS             complement(2291..3247)
                     /locus_tag="LP859_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL3030365.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LP859_002466"
     CDS             3528..5462
                     /locus_tag="LP859_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3030366.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LP859_002467"
     CDS             5919..6335
                     /locus_tag="LP859_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL3030367.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LP859_002468"
     CDS             6349..6915
                     /locus_tag="LP859_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL3030368.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LP859_002469"
     CDS             6938..7924
                     /locus_tag="LP859_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL3030369.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="LP859_002470"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="LP859_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL3030370.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1658 c         1750 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DAFEZB010000092         8490 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFEZB010000092
VERSION     DAFEZB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.092"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..173)
                     /locus_tag="LP859_002471"
     CDS             complement(<1..173)
                     /locus_tag="LP859_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3030371.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LP859_002472"
     CDS             complement(593..1714)
                     /locus_tag="LP859_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL3030372.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LP859_002473"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LP859_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL3030373.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LP859_002474"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LP859_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL3030374.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LP859_002475"
     CDS             complement(4304..5215)
                     /locus_tag="LP859_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3030375.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LP859_002476"
     CDS             complement(5232..6239)
                     /locus_tag="LP859_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL3030376.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LP859_002477"
     CDS             complement(6236..8365)
                     /locus_tag="LP859_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3030377.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="LP859_002478"
     CDS             complement(8370..>8490)
                     /locus_tag="LP859_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3030378.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DAFEZB010000093         8323 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFEZB010000093
VERSION     DAFEZB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.093"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="LP859_002479"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="LP859_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL3030379.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="LP859_002480"
     CDS             complement(738..1460)
                     /locus_tag="LP859_002480"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL3030380.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="LP859_002481"
     CDS             complement(1596..2477)
                     /locus_tag="LP859_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL3030381.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP859_002482"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP859_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL3030382.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="LP859_002483"
     CDS             3717..4196
                     /locus_tag="LP859_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL3030383.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="LP859_002484"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="LP859_002484"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL3030384.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="LP859_002485"
     CDS             5497..5625
                     /locus_tag="LP859_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL3030385.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5629..6174
                     /locus_tag="LP859_002486"
     CDS             5629..6174
                     /locus_tag="LP859_002486"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL3030386.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LP859_002487"
     CDS             complement(6628..8319)
                     /locus_tag="LP859_002487"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL3030387.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2300 a         1692 c         1517 g         2814 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaaccca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc ttt
//
LOCUS       DAFEZB010000094         8298 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFEZB010000094
VERSION     DAFEZB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.094"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            47..679
                     /locus_tag="LP859_002488"
     CDS             47..679
                     /locus_tag="LP859_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL3030388.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="LP859_002489"
     CDS             complement(681..1586)
                     /locus_tag="LP859_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3030389.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2330)
                     /locus_tag="LP859_002490"
     CDS             complement(1671..2330)
                     /locus_tag="LP859_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL3030390.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="LP859_002491"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="LP859_002491"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL3030391.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="LP859_002492"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="LP859_002492"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL3030392.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="LP859_002493"
     CDS             4573..5658
                     /locus_tag="LP859_002493"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL3030393.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="LP859_002494"
     CDS             5655..6719
                     /locus_tag="LP859_002494"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL3030394.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="LP859_002495"
     CDS             complement(6968..7771)
                     /locus_tag="LP859_002495"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL3030395.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="LP859_002496"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="LP859_002496"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8298)
                     /locus_tag="LP859_002497"
     CDS             complement(8078..>8298)
                     /locus_tag="LP859_002497"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL3030396.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2505 a         1740 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgccc
//
LOCUS       DAFEZB010000095         8290 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFEZB010000095
VERSION     DAFEZB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.095"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(45..1511)
                     /locus_tag="LP859_002498"
     CDS             complement(45..1511)
                     /locus_tag="LP859_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL3030397.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="LP859_002499"
     CDS             1898..2494
                     /locus_tag="LP859_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL3030398.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="LP859_002500"
     CDS             complement(2552..3034)
                     /locus_tag="LP859_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030399.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="LP859_002501"
     CDS             complement(3140..3337)
                     /locus_tag="LP859_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030400.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="LP859_002502"
     CDS             3632..4390
                     /locus_tag="LP859_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL3030401.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="LP859_002503"
     CDS             4450..4878
                     /locus_tag="LP859_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030402.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="LP859_002504"
     tRNA            4924..4996
                     /locus_tag="LP859_002504"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="LP859_002505"
     CDS             complement(5146..5517)
                     /locus_tag="LP859_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3030403.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="LP859_002506"
     CDS             5732..5917
                     /locus_tag="LP859_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030404.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="LP859_002507"
     CDS             complement(6000..6188)
                     /locus_tag="LP859_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030405.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="LP859_002508"
     CDS             complement(6212..6475)
                     /locus_tag="LP859_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030406.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="LP859_002509"
     CDS             6633..6890
                     /locus_tag="LP859_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL3030407.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="LP859_002510"
     CDS             complement(6961..7152)
                     /locus_tag="LP859_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030408.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="LP859_002511"
     CDS             complement(7293..7613)
                     /locus_tag="LP859_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030409.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="LP859_002512"
     CDS             complement(7636..8070)
                     /locus_tag="LP859_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3030410.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DAFEZB010000096         8251 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFEZB010000096
VERSION     DAFEZB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.096"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..197
                     /locus_tag="LP859_002513"
     CDS             <1..197
                     /locus_tag="LP859_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030411.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LP859_002514"
     CDS             314..811
                     /locus_tag="LP859_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3030412.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LP859_002515"
     CDS             898..1290
                     /locus_tag="LP859_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3030413.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="LP859_002516"
     CDS             1274..3721
                     /locus_tag="LP859_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3030414.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="LP859_002517"
     CDS             3724..5907
                     /locus_tag="LP859_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL3030415.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="LP859_002518"
     CDS             5904..6905
                     /locus_tag="LP859_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL3030416.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="LP859_002519"
     CDS             6920..7834
                     /locus_tag="LP859_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3030417.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="LP859_002520"
     CDS             8079..8195
                     /locus_tag="LP859_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL3030418.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DAFEZB010000097         8066 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFEZB010000097
VERSION     DAFEZB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.097"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..882
                     /locus_tag="LP859_002521"
     CDS             <1..882
                     /locus_tag="LP859_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL3030419.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            930..3059
                     /locus_tag="LP859_002522"
     CDS             930..3059
                     /locus_tag="LP859_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL3030420.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3034..3723
                     /locus_tag="LP859_002523"
     CDS             3034..3723
                     /locus_tag="LP859_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030421.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4131..4817)
                     /locus_tag="LP859_002524"
     CDS             complement(4131..4817)
                     /locus_tag="LP859_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3030422.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4953..5147)
                     /locus_tag="LP859_002525"
     CDS             complement(4953..5147)
                     /locus_tag="LP859_002525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030423.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="LP859_002526"
     CDS             complement(5197..5637)
                     /locus_tag="LP859_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL3030424.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="LP859_002527"
     CDS             complement(5641..6486)
                     /locus_tag="LP859_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL3030425.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="LP859_002528"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="LP859_002528"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL3030426.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2357 t
ORIGIN      
        1 atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct
       61 aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa
      121 agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt
      181 gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca
      241 aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca
      301 gttttagggg tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc
      361 tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt
      421 gcgaaattat ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc
      481 ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga
      541 gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta
      601 ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg
      661 ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg
      721 attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca
      781 aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg
      841 atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa
      901 aaacgattag ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg
      961 ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa
     1021 aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt
     1081 cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac
     1141 attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa
     1201 tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg
     1261 atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa
     1321 acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg
     1381 tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg
     1441 aagcagcatc tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg
     1501 gattgacaag aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag
     1561 atacggcaac ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga
     1621 ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa
     1681 ccatgctttc cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta
     1741 atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac
     1801 gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct tcattagatg
     1861 ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg
     1921 atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt acggagacag
     1981 aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt
     2041 acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca
     2101 atgttcttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa
     2161 aagccataca ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga
     2221 tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc
     2281 acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta
     2341 cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt
     2401 cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc
     2461 caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag
     2521 gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa
     2581 cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa
     2641 cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct
     2701 tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg
     2761 gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat
     2821 gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg
     2881 cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag
     2941 tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat
     3001 ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag
     3061 ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg attctaccag
     3121 tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa
     3181 caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc
     3241 gatgtaggag aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa
     3301 ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg
     3361 gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa
     3421 gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat
     3481 acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt
     3541 cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt
     3601 tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg
     3661 atcttagaag aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa
     3721 taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc
     3781 ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa
     3841 attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc
     3901 aaaagcttgc atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta
     3961 cttggctgtt cttattactt gatgcagggc agctttttca caacctctca ataaacggtg
     4021 tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt
     4081 tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg
     4141 ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc
     4201 tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca
     4261 atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa
     4321 atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc
     4381 caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DAFEZB010000098         7901 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFEZB010000098
VERSION     DAFEZB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.098"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..871)
                     /locus_tag="LP859_002529"
     CDS             complement(<1..871)
                     /locus_tag="LP859_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3030427.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANY"
     gene            complement(871..1389)
                     /locus_tag="LP859_002530"
     CDS             complement(871..1389)
                     /locus_tag="LP859_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030428.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1405..1731)
                     /locus_tag="LP859_002531"
     CDS             complement(1405..1731)
                     /locus_tag="LP859_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL3030429.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(1744..3723)
                     /locus_tag="LP859_002532"
     CDS             complement(1744..3723)
                     /locus_tag="LP859_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3030430.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(3744..4058)
                     /locus_tag="LP859_002533"
     CDS             complement(3744..4058)
                     /locus_tag="LP859_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL3030431.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4226..4519
                     /locus_tag="LP859_002534"
     CDS             4226..4519
                     /locus_tag="LP859_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL3030432.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(4597..5709)
                     /locus_tag="LP859_002535"
     CDS             complement(4597..5709)
                     /locus_tag="LP859_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3030433.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            5910..6086
                     /locus_tag="LP859_002536"
     CDS             5910..6086
                     /locus_tag="LP859_002536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030434.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            6096..6275
                     /locus_tag="LP859_002537"
     CDS             6096..6275
                     /locus_tag="LP859_002537"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030435.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6469..6639
                     /locus_tag="LP859_002538"
     CDS             6469..6639
                     /locus_tag="LP859_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030436.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7063..>7901)
                     /locus_tag="LP859_002539"
     CDS             complement(7063..>7901)
                     /locus_tag="LP859_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3030437.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         2454 a         1619 c         1286 g         2542 t
ORIGIN      
        1 gataatttgc gccttgtgcg ttagctgcca tattagctaa tacgatcggg gtcaagaaag
       61 aagtgatgat attcgctcca tggatcccta cgatccacaa tgcatggatc aagaaataaa
      121 tcaccatgac accaagccat gtattcgtca actgcgtaac aaacccaaat gggatagcaa
      181 tcattttgaa gatatccgtt cctagtgcaa ctaataatcc attgatgatc atcaccacaa
      241 acgcaatgac aaatgttggg atcagagcag tgaaagaacg agaaactcct ggcggaacaa
      301 catctggcat ttttatgacc caatttctct tgacacacat acaatacaat tcgaccgcaa
      361 taatcgacat aataatagct gtaaagattc ctgaagttcc taaacgttcg acaaaagagc
      421 ccatcctaat accatttact acagtttctg tctcattgat agaagcaatc aagctgattt
      481 taccatcagt catgatcaat tcagggatac acataaagaa tgcaaaaaca gatagtaatg
      541 cccccgtgag cggattaaca tttaattttt gttcatctcg ttcaattgac gttaattcat
      601 aagcaaacac aatggcaaag taaatagata aaatccccat cgtcattgta ttgacgatca
      661 tgtacaagtc actaactttg aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg
      721 gtaatggcaa aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag
      781 ccatccccgc tgccatgaca gcccgaacga ttttaagttg cgcaattttc cccataggtc
      841 ccatcaggta tttctgcaaa aaatcaaaca ttagttattc tcctttccta aatataacgc
      901 tttttcatat tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa
      961 acgatactgt ataaatgcac tggcaaaaat accgcttaca acaagaatga aaacaaataa
     1021 ctgtcctcca ttgttatttg ccataaaagg atacaagaat gtaaatcctg aggtaaacaa
     1081 cactggaatc actaataaat taaagacact ctggaacaaa aaataccact ttgtccatgg
     1141 aacgttcgtt tggtgttttc gataaagctt tacttgttct cctactgcag gcaaaatagc
     1201 taaaatcaga atcatcggta aactacttcc taaactttta gctgatagaa gcagaatcat
     1261 ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta gataacgatt
     1321 gaaatacatg tttttcaaac taagttgctt tttttctaat tcgattttcc tttttactgt
     1381 atcctgcaca atgatccttc ctttctattt atttttttga tacaaataat tcatttcctg
     1441 tgcaacttcc aataatgtca tcgtcgtcat cagatgatct tgtgcatgga ccatgataat
     1501 ttccatttct atttttgtcc cgctggcata ttctttcaga agttgagttt gtgactgatg
     1561 cgcatgaaga atctcttcat ttgcttgtcg caacttctct tctacttctt caaacatctc
     1621 tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc tatggaaaat
     1681 gatatcaaaa gaagtcactt gtagtgtttc actgtttatt ttttcagcca tctgttttcc
     1741 tccttattcg atcagtttga taaatttact gctgaaattc tcaaaaatag gttcagctaa
     1801 gatttcctgc tgtactttta gctggtcaac taacctaacg attgctttag tcacgaccgt
     1861 aattccttca ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa tttcttgata
     1921 ttgatcccac tgcatcccag aaggaatcaa tgcaacggct acctttgttt cgattccaac
     1981 tggaacagcg ggatgtggga ctgccactgt atcactgaat atgatttgtc ccatattttc
     2041 acgttgctgg atctgctttc gcatttctct tatatatcct tcttcttcat ttatgctcag
     2101 cttttgcagc aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa
     2161 aaatttttca gacaactgtt cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg
     2221 tatcggtcta tctgaggaat gatagggtat agattctcca actacctttc gtattttacg
     2281 aacatcatct tcattcaaaa aaatactgac atgtaaaact ggaattttga aaaacattgt
     2341 cgaaagatca atagaagaaa taatcaggtc gattcctttt aaagttgttt cattgatctc
     2401 ataatagcct tttacactta ccactgtaat atttttaccg aattcactga ctacgcgatt
     2461 ctttaatagt tgggcactgc cgtaacccgt tgcacagata atcaatgcat gtagtttatg
     2521 cgcagctctt tctttttcca atgccgccat gagatgcaaa gccaaatagg cccattcatc
     2581 ttcattgatg gtatagtttt tcaaagccgg catgttgctg aaatgccttt ttactgcgtc
     2641 aaagactttg ggattttctt tctttatctc ctcagtcaat gggttctcaa tgcgaatacc
     2701 tcggctcaat cgaatcatca tcggtgacaa atgatcgagt agtccattga aaagctggag
     2761 atcctctact aatgaaaaat gaaattcctg agacaatctt ttcagaactt tcgctaattc
     2821 ctttgatagt tcctgatcat tttgattttc tttgtgattc gacttagcca tgagatgtaa
     2881 tgtcaaataa gcaatttctt cttcgggaaa cgatacacct gcgattacct caatacgttg
     2941 aacgatccgc tgcgccactt gatactctac tcgcttagaa acttcttgtt cgatgcctaa
     3001 ttccttgatt tcaaaccctt ctcggagccg tttgatcccc aaagccaagt gtaaagtcag
     3061 attctgtatg ataaaatccg aaacttttaa cttagcttct cttgtttcat cgagaatgat
     3121 aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt
     3181 caatgaattt ccataatggt ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct
     3241 ttcttcccct tcgatccatg tcccttgacc atgcttagaa acgattttta gtgaatatgg
     3301 aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag
     3361 ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat
     3421 atacttttgt cgatcttcgg actcataaaa tccttgcggt tctctagcat atccaggtac
     3481 tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta
     3541 gccataacct ggtttagcaa tgatcttacc tccgtttttt tctactaact ctttgatccg
     3601 ctgcaagtaa ttacggattg tccgatcaga taataacaat tcagaagcta aacgttgact
     3661 ggtgacatac tccttcttgt gatcaatcaa atatctcaac agcaattttt ccttctgttt
     3721 cattatcatc cactcctatt ccatcatatg ttctcttcga tcacctctgc taacttctgg
     3781 atgcctgtag ggattgggac atacgcttgg aaagggatac taacaacagg ctttccaaca
     3841 gattctccta attttttgaa tttatccaaa aacatcgtcg tttgcgggct gatcagaaat
     3901 aaatcaaaat cactattctt aatcatatta tcgccttctg tagctgaaac tgcatctacg
     3961 agtacctctt ctcttttttg cttaaagaaa tccgttgtct ttgaggccat catcgaggat
     4021 gacattcctg ccgcacaaat aatcaatgct tttttcataa atcaatcact ccttttattt
     4081 ttataaacca agcatatctt gtaagcgttt tctattcaaa atatatattt ccgtagcatt
     4141 gcggaaaaaa tgtgtatcat atcttgaata ataatttctg gcaccgtgat atactagtga
     4201 tagaattttt tcaggagggg atttagtgag tatcacagtg aatattttgt atacaagtac
     4261 aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat
     4321 tcgtgcagaa gaaggaaacg agaaatatga ctattacttt ccactagaag atccagaatc
     4381 tcttttactg atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc acaaatccga
     4441 aatgatggct caaataaacg aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg
     4501 tttcactgat ttaccgtaaa aagatacaca tggaatgata gcaatttatc atccatgtgt
     4561 atcttcactt tttccttatt ttttattcga tctcatctaa tgattcaaac cgtgtttttc
     4621 tcttattttt cggataagta tttcagtcac cccaatatac atgattccta aacatacccc
     4681 aaggatattc tcaaaaatgc ggatcgttac tgctcctgga accccgaata aactggcagt
     4741 gattgccaaa gcaccaaaag aattaaaaac tgttttatat ctataggtag aacaaacacc
     4801 taaagctaga ccacctaata taccgaccca gttaaaagga ataaattggc tgataccaat
     4861 aaataaaaca catccaatca atgagccaat gattcggtct actgctcgtt ctttgaaacc
     4921 aatcgacatc aatccatagc tagatacaat agaagaaaat gcaaaccctg cccacatcaa
     4981 tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat
     5041 gagcatccaa attttttcct gcgaatacat gcctttcaaa aaaatctctt taaacaagct
     5101 tttgtctttg ttcttttctc gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa
     5161 caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa
     5221 cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc
     5281 actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac
     5341 tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa
     5401 acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat
     5461 acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg taattataat
     5521 gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata
     5581 catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc
     5641 tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt
     5701 tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct
     5761 tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt
     5821 aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa
     5881 aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac
     5941 agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa
     6001 attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga
     6061 aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa
     6121 agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc
     6181 gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa
     6241 aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca
     6301 cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt
     6361 tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc
     6421 ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta
     6481 acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg
     6541 aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat
     6601 cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg
     6661 aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa
     6721 actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac
     6781 ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt
     6841 gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc acttctcaac
     6901 gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct actccgatac
     6961 taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta
     7021 gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt
     7081 tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata taaactggct
     7141 aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc
     7201 tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc
     7261 ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc
     7321 ttgagtttct accagctgat atgctcctgg tgctaagcca tctatcgcca gttttccatc
     7381 tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac
     7441 agctccttgc aggacttctc cgctttggtc atcgatcttt gtcaacacga caccgcctgg
     7501 tgtaagtcga ttttctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa
     7561 ctcgattggt gtcgcatcca attcataccc cgttggcgct tgggtttcta ccagctgata
     7621 cactcctggt gctaagccgt caatcgccag ttttccatct gctcctgtgg ttagacctga
     7681 ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca gctccttgca ggacttcccc
     7741 gctttggtca tcgatctttg tcaacacgac accgcctggt gtaagtcggt ttgctttcgt
     7801 caattctacc accgctgtct gacttctttc gatctcaaac tcgatcggtg tcgcatctaa
     7861 ttcatacccc gttggcgctt gagtttctac cagctgatat g
//
LOCUS       DAFEZB010000099         7871 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFEZB010000099
VERSION     DAFEZB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.099"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(663..2009)
                     /gene="gor"
                     /locus_tag="LP859_002540"
     CDS             complement(663..2009)
                     /gene="gor"
                     /locus_tag="LP859_002540"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL3030438.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2143..3396)
                     /locus_tag="LP859_002541"
     CDS             complement(2143..3396)
                     /locus_tag="LP859_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL3030439.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3402..4325)
                     /locus_tag="LP859_002542"
     CDS             complement(3402..4325)
                     /locus_tag="LP859_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL3030440.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4546..7038)
                     /locus_tag="LP859_002543"
     CDS             complement(4546..7038)
                     /locus_tag="LP859_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL3030441.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7045..7512)
                     /locus_tag="LP859_002544"
     CDS             complement(7045..7512)
                     /locus_tag="LP859_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL3030442.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7648..7721)
                     /locus_tag="LP859_002545"
     tRNA            complement(7648..7721)
                     /locus_tag="LP859_002545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7685..7687),aa:Arg,seq:acg)
     gene            complement(7779..7852)
                     /locus_tag="LP859_002546"
     tRNA            complement(7779..7852)
                     /locus_tag="LP859_002546"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7816..7818),aa:Pro,seq:tgg)
BASE COUNT         2195 a         1706 c         1268 g         2702 t
ORIGIN      
        1 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
       61 tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt aaaatcagtg
      121 ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
      181 tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag tgactaacta
      241 tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc agcgaacttg
      301 acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc gtttgcttct
      361 tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat acatttctat
      421 ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa tggattcatt
      481 tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta tttcgttttg
      541 agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga aaatcagtat
      601 actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact gttaatatta
      661 aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac tgtattgtca
      721 aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag catctcatcg
      781 acaccaacac caatagcatg taagccgacg attctttctt cttttccgac acagatcaat
      841 ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt aaagcgtgaa
      901 cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc tgtcaaacca
      961 attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata gtcaagataa
     1021 gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc gattgcaaca
     1081 ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc atccgttgta
     1141 ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt tgtattttcc
     1201 agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca atcaccagtt
     1261 aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt aatttcttta
     1321 gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat caaattgtcc
     1381 gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca catgacttca
     1441 ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc acctaaaacg
     1501 attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat tgcgtattct
     1561 ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat tttaggagca
     1621 cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc gtaacctttt
     1681 atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag aaaatcaata
     1741 tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc tgcttggata
     1801 ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg ccacataact
     1861 tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc ttcaatcagc
     1921 aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc agagccgcca
     1981 ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa
     2041 aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt cttgaatttt
     2101 gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac gttttcggat
     2161 catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca ttggatttgg
     2221 tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt gactgaaatg
     2281 actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt ttctctgacg
     2341 tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa atttatatac
     2401 tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat tgtagtaaaa
     2461 acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt cttgcttgca
     2521 gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga catttgacac
     2581 cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt ctacgccgtt
     2641 ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca tcgagaattc
     2701 ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag gcatatcgaa
     2761 caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat cccgcatcga
     2821 catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac catggataaa
     2881 tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca ttgaaacttc
     2941 acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg tctcaaaatt
     3001 agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg tcgcacaaat
     3061 ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac gcaaactgcg
     3121 gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag cagcaacgta
     3181 taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt cataagtaaa
     3241 attacctgca cggcttctta aaccatatgc attcccgcca atatatactg catctgctcc
     3301 ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga tttctggtcg
     3361 tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat agcagaagga
     3421 tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt actgatttcg
     3481 ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg tttttttgct
     3541 tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa tccgtccaat
     3601 ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag attcagatca
     3661 ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg tgtctcttct
     3721 tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc ttgtttcgta
     3781 tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt agcaccatac
     3841 acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc ttcaaaagga
     3901 acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt gatttgacgt
     3961 gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc aggatttttt
     4021 ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt tatttctttt
     4081 aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat cccatttaca
     4141 gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg ctgttcttct
     4201 cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata tagtgtatct
     4261 gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt tatgagttcg
     4321 atcataattt tctcctccta caacttcata atagtagcat agctctaaaa actttccaat
     4381 acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca aaataaagtt
     4441 cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca caacctctta
     4501 atcaagtctt actattataa aaattcagct acacaggcca agtaactata gggaacctac
     4561 tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag cacctactgt
     4621 tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat
     4681 ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa atccagcttt
     4741 cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt ctttcaatcg
     4801 tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt tactcaatga
     4861 atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag ctttctttaa
     4921 ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt ggacattgaa
     4981 tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca taatcatgat
     5041 tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat catccaatac
     5101 ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta agagaatcac
     5161 tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat accctacgta
     5221 tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt ctgacatatc
     5281 aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt tggccaattc
     5341 tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac gatcaggatc
     5401 tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag cctcatcttg
     5461 accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc tttctttttt
     5521 ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa catcttcttc
     5581 tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa ctttttgcag
     5641 ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa agtcttgttt
     5701 ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga tcacagttga
     5761 ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat cgatcaaatc
     5821 aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat tgactgctgc
     5881 tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg tacgtaatcc
     5941 ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc gagcaaaacg
     6001 tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg tcgttgctcc
     6061 gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag catcaattgc
     6121 tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata agattacttg
     6181 accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt caccgcgata
     6241 tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt cttgcatgtc
     6301 ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg ctgttttgcc
     6361 tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa gaatctggat
     6421 aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt cttttgctaa
     6481 tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt
     6541 atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat caccagctag
     6601 ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta agttgatcat
     6661 gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat gttctgttcc
     6721 aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa cttgtttcat
     6781 tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac cataaccaat
     6841 cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac gtaatacttt
     6901 tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg aactaactga
     6961 ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt gggctctttc
     7021 agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac gttccagaaa
     7081 tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca atgtgttttt
     7141 tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt cttcataaag
     7201 tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata actgatttat
     7261 ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta tataaccacc
     7321 accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg tattaatcac
     7381 atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc
     7441 gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat ccgaggtatt
     7501 ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat aacgaatttg
     7561 ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc aacaaaaaaa
     7621 aatacggaat atactattcc gtatcgacgc gcccaagagg agtcgaaccc ctaacctttt
     7681 gatccgtagt caaacactct atccagttga gctatgggcg catattcaat ttaaaaaaat
     7741 tataaatcat ttgaaaacaa aaagcaagat aaaaaaaatc gggaagacag gattcgaacc
     7801 tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc cgtttaaaaa
     7861 ataaaaaaga c
//
LOCUS       DAFEZB010000100         7826 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFEZB010000100
VERSION     DAFEZB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.100"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..1127)
                     /locus_tag="LP859_002547"
     CDS             complement(<1..1127)
                     /locus_tag="LP859_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL3030443.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
     gene            complement(1163..1873)
                     /locus_tag="LP859_002548"
     CDS             complement(1163..1873)
                     /locus_tag="LP859_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3030444.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1941..2756)
                     /locus_tag="LP859_002549"
     CDS             complement(1941..2756)
                     /locus_tag="LP859_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBL3030445.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2767..3735)
                     /locus_tag="LP859_002550"
     CDS             complement(2767..3735)
                     /locus_tag="LP859_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3030446.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            complement(4468..5670)
                     /locus_tag="LP859_002551"
     CDS             complement(4468..5670)
                     /locus_tag="LP859_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HBL3030447.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            5691..6464
                     /locus_tag="LP859_002552"
                     /pseudo
     CDS             5691..6464
                     /locus_tag="LP859_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            6643..>7826
                     /locus_tag="LP859_002553"
     CDS             6643..>7826
                     /locus_tag="LP859_002553"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030448.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHT"
BASE COUNT         2401 a         1591 c         1236 g         2598 t
ORIGIN      
        1 ttttttataa cagttgacat gtaagcctct tctaacgttt gattcaagcc gagaatttgt
       61 ggatatacct ctgctactac ttgtccttcg ttcatttttt ctgctgtctt ttgagtaacc
      121 atttccaaat cactaatcga attaaaaacg ccttcaggta accgctttcc aaattcgaca
      181 agaattgacg cagctttata acccgcttta tctccttccg ttgtcaaaca gcaaggaatc
      241 tctacttcat tgaatgcagc aacttctgct ttaattgcct catagtcaat ctcgggtgaa
      301 gctactcctg actgataaac accgtaaata tctccacttc tatccgcaac aacaccatct
      361 accatgatct tcactccacc aaagcggacc ttttcaggga aaactttttc cttatactca
      421 tatagaagtt catgctgtag aggttcttgg actgcctgca catataggtt actaagtatt
      481 ttcctctgag gtatattaga aagaaattct gcgtcatcaa aaacgatttc tttcattgat
      541 ataacacctt ttgaaagcag taatgcttca aattcagcgt aaacctctgc taaaagcttt
      601 ttgtttgcta gcatttcctt aattaataat actcgacttt cagcactggt ttctttttct
      661 gtaaaaccat aagtctgctt agcaatagca ttcatccaac aatggctgcg atttttgtcg
      721 atcgctgtga ttggaccaaa gtggtttatt tcattcaata gctcttctga tgggtcagtt
      781 ttccacaggg cagcatccca gccatgagca taaattggtt catctgcttt tttatggttc
      841 attacagctt taatacgctc tacagcctcg ttagctgaag atgtatcgct taaatctatg
      901 ccagcattca tagccaaata accactgaaa aatacatgat tatcatgaat tgctggcata
      961 acgaattgat catcataagc aattaattcc gtgttttcat ttaatagatc actgtaattc
     1021 cagttgtttt ccaccttttc aatgatccct tcattgatta caatataacc tttgaaaggt
     1081 ggctgctctc catcaaaaat catttttgac cgcaatattt gcttcataaa taaaatctac
     1141 tccttgaaat caaaacagga aatcataagt aaaaacgata tacttctccc aggtaatatt
     1201 gattacctac atgaattggt atgttgtttt gatcgttgat taaagtatcc ataaagaaaa
     1261 agggcgtccc aacagctact tccattattt tggcaatttg gtcatcagct gtgacaattt
     1321 caagtgcagt ttccccagga ttgatggcta aggtaccttg gactttaagt acttcataca
     1381 aagaatcagt cagctcttcc tccattaaga aactatatct ttcataagaa aaataattgt
     1441 tttctaacat aatggggatg ttatccgcca ttcgctttct ctggatatat aaaacatcat
     1501 caccttgagc gatatcgagt ttttttgcca tcccttcagt tgctggaatg atcttgcgtt
     1561 ccaaaacaat gctagtcggt gtaaaaccat tagatttaca actctcactg aaacccataa
     1621 catattcaat tttacgaaaa actttatgcc ggttgacaaa agttcctttc ccttgttttt
     1681 taagtaggta cccttcttct accagttctt ctactgcttt tctcactgtg attcggctga
     1741 cctcataact ttctatcagc tctgattcag cgggaatttt cgtattctcg ggatactccc
     1801 ctgtattgat tttatttttt aatatttctt ttaattgtaa gtacaatggt aattgtgcat
     1861 ttgtatccag cattttttta acctccactt taccgggatt tctttcttta ggttatcaaa
     1921 tgcaggaatt taaaacaagc tcaatcgtaa tgaataccaa agccgaacga accgtcaacc
     1981 agacaagact tcccagcaaa ttcattcccc tttttcatcg cggtgactat atcgtcccct
     2041 tcgagaaaat aaagtaggaa agctgttaag aaagaatctc ccgctgccat tgtatcaact
     2101 ggttcttcta gcacggggtt cacttcataa aactttgctc catcataacc aatagccgtt
     2161 ttttctcctc gggttgcaat caatacatca tttcgctcac aaaaatgctt catcaaaaac
     2221 tgctttactt cttcatcgga aaagtcttct atagaaaaga agccaaaatc aactttatca
     2281 attgcccgct gaacctcttc ttccgtaaag tcgtcagaaa aatcataaga aatctttcca
     2341 gaaacatttt gtaactcttc taatagatga ttgacatggc tatataatcc catatgaatc
     2401 aattcaaact ggtttaaata ttctctgtct tcttcagtaa gctgaatcgg gtattcacga
     2461 gtaacacctc ccgaattgct gccaaggaaa atccggtcgc cctcatcaat cttcactctg
     2521 gcacagccat tctcgccttt ttcaaagcga gagtgactgt tgtccagtcc tatttctttt
     2581 attgctgata aaacatgagc tgcctcctca tctgagccaa aaactcccat aaagctactt
     2641 tcatgtccca gtttttttgc aaaaactgca aaattcaatg aatttcctcc aggatacata
     2701 attcccagat tttcatattt atcgacgacg ttatcaccta agcctaatac cttcatttta
     2761 atcctcctag tactccactt tccccatata gcgacgaaca tctaaatcat gattgcgtgc
     2821 atctgctaaa gctgaacgat attctgaaag aatactgtag aataaaatgg gattgaagaa
     2881 ttcagaaact tcatctgata tcatatccaa tcctagttcc tttgcatcaa cgatttctac
     2941 tctttctgca tatttatcaa ggaatcgttt tgcccgttca tctaaagcac gagtgcgtcc
     3001 aatgctcatc aagagaataa aattcgtatc tttgtctgtt acttcaaaag gtccatggaa
     3061 gaattctcca gaatgaacag aagttgaatc aagccattgc atttccatca aagagcaaat
     3121 tgaaaaacca taagcatgtc catatgatgg tccactacct aagatataaa acattgtttc
     3181 atctttgtac ttatcagcaa acactttaat acgatcctgc acctgcttaa tgccattttc
     3241 aattacggta tcgataacct tcatgccttc tctgaattta ccgtaatgtt caaatgtttc
     3301 ggtaacattg accaaagata cagttaaatc aagaatgatg gccataggat tattgactat
     3361 ttttgcatca tctccccatt cgtaaaggaa actatgatca gaaaccttta ataaatccgc
     3421 agttttatta tgagtcagac caacagtcac agctcctcta gcttgagcga cttctcctgc
     3481 ttttaccgtt tctgctgtat ttcctccatg agaacataga atgacaacag atttatcatc
     3541 taattgcttt ggggttacat ggttaaattc gttcgctgta tataagcctg taatacactt
     3601 cgatgcttcg gctttaagaa aataatctga gacatacata tctaccagcg aaccgccaca
     3661 agccaccaaa taaattccag aaatctctcc atcatttttt tctacaattg cgttaaccgt
     3721 ttttgctaca tccatcaaca tttcctcctt aaaattttaa aaatgttaat tttatccaaa
     3781 ataaaagtaa ctgattaaat gtaaagatat tacataacgt catataacgt catgtgacat
     3841 aattgtattg tatccgcttt ctttgtgttt gtcaatagga aaaatttatt tctctaaaaa
     3901 atataaataa tctgcagtga aacttgagta catatactaa tttcgaaaaa tgcctatgat
     3961 ttaaataatt atggtgctgg gatgaaggtt tataagtttg tcttgatttg tttagaagaa
     4021 atcgttttgc acacagttga attaggaaac tactggagtg gaaaataaag tgatttgacg
     4081 ggtagatggg tgcacttaaa tttgaaaatg gcttaaaatg ctttattttg aattgcagaa
     4141 tttcttttag tacgcaattt atggtcttaa aattgattaa aaagtgatac attgatttta
     4201 atacaattta tgatacaaaa aatgacctcc tttcttaaat aaaagaggtc attttgtttt
     4261 gtctcataat cgattgtttt tgatacagta gttatattca gaaaaaataa agactcttct
     4321 gagtttataa tcctttggat tatatacgga tacagatgta gacataaaac aagctcaatt
     4381 ttcaaaaatg gcattactca cctttttggt gaattattaa actaaaaaag atgaattttt
     4441 ttatgtacta taagatgtag gtgtactcta gttaaagtaa ttctttatgt actctgtaac
     4501 atcgaataca tcttctaata aaaatgcacg caataatctg tcaattagca tatttacctt
     4561 gattggtatt accattttac ctaaattttt ctcgatatat ccactgaaat gacctacgtt
     4621 aacacttgat tttaatttat caaatcgact atattcatta atgtttacct cggttaagcg
     4681 taaatccaac atctcacgta ataatgcctc attaatattg gttgaatcca ctaaatcttg
     4741 gataaaatcg tcctcttcac taagctgata ttcattgagg taatcagtaa attcccatcc
     4801 ttctttgata actaattccc cattatttac atcatttaga atcatctcag catatggctg
     4861 taactccact ggtaattgcg caaatgtgcg atgtaagtcg tttaacaatg attgaattaa
     4921 attcgaatca cgttcttctt ttatattcat gtattttttg aagtttgtgt ttaaatactc
     4981 ataattaata cgactacgac taccttcagg taattggtaa tcaatgtcat acgctatact
     5041 atcatttccg tctctaggtt ctctctctat ctcgctgtaa cgttgtgcca agatattaaa
     5101 ttcatattct gtgattacta ctgttatagg ttcataatcc tggatttcat aagtcgattt
     5161 tgcccatgtg aatccctgta tcatcgctga ttcataacaa tggattagtt gattataaag
     5221 atttacaaat tgcttaattt cccctggaac ctccggtaaa tgactgaagt tttctatgcc
     5281 agaaatatta aatacggact taatttcttc gaatatatcg ttcaactttt ctaagttatt
     5341 atataatttt tcaacaaata acccttttgc ttgtttgcct gagtataact caatagcctc
     5401 ttcaatgttc tgttccatcg tatgtgcttt acgataataa cgaatggttc cgaacggttt
     5461 ttcaggacca ttaagacgat ttgtgcgtga aaaagcctga attatgttct gatattcaag
     5521 tgacttatct aagtataggg tgttgaccca ttttgaatcg taacccgtta agagctgatt
     5581 aacaactata acaagatcta attgggttgc ttcatctact gttttgtaag gattcttatg
     5641 cgctgtgttt tgcaatagtt ttgtacacaa gattgcaaag aaaagtacac aatgttgcca
     5701 cttctggttc catgagtttt atttgtgttg atgggacaac caatcagcta tttacgaccc
     5761 ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct agaaaggccc
     5821 gtttaaaggt caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa
     5881 cggtctttcc aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc
     5941 gcagcttcaa tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa
     6001 atcagaagaa gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga
     6061 catgggaggc actcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag
     6121 aaatcgttaa tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc
     6181 aattattacg ctattatttc gttaaacgag atacggtagg ctttcaagaa acccttaaaa
     6241 caatttcatc tacgttacct cagtcgttta aaaagaaatt cacgatcttc catcgctacc
     6301 aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa cggcgtcact gaggggctgt
     6361 ataacaagat caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc
     6421 gggcacggat ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca
     6481 cagaacgcgc cgcatatcgg actaagtttt attggctcta taatttctag gactgatgga
     6541 aattttctat ctgtcctagt ttttttgtaa aaaaacaaaa agacatacaa aatttccggt
     6601 agaatagagt ctcctacaac atcattctgg gggaattctt gtatgtccat gaatcatttt
     6661 atcaaacaac tctttactat taaagactca aatatccaaa ttcaatcctt tgaagaaact
     6721 ttttataaaa gcagaaaaag tatcgttatt gagggaactc tgatccgtac ctacagacgt
     6781 tgtccgtgtt gtaaagattc tacaaagcaa atcgttaaaa atggcaagaa gatctctatg
     6841 attctcctga atcgtagcgg taataaacgg acctatcttc ggttgaagaa acagcgatac
     6901 ctctgccgag catgtaaaaa atattttacc gctcggacgt atctcgttac cccattctgc
     6961 tttatttcta agcagattca ttacaagatc ttagaagaat tgacagagcg tcaatctata
     7021 aaagcaattg gtaaacattg cgatgtttct gtgacaactg ttcagcggac attgtccgcc
     7081 ttgtcatcag atatccgtca gaatcttgag tatttacctc ggtgtctatt attcgatgag
     7141 tttcgttctc tttctacatg gcacggtaaa ttttcttttt cttgtatgga cggtgagacc
     7201 gggaaactgt ttgagatctt acctagccgc cgtaaaaaag atctcgttgc ttttttcatg
     7261 cgtttttctc tcaaagcgag gttgggtgta cattacatcg taacggatat gaacgctcct
     7321 tatttctcac tagttaaaga gtgttttcca aatgcacaag tgatcgtcga tcggtttcac
     7381 attgttcaac atctgaaccg cttcttcgat gcggttcgaa aacgtgtgat gaaaacactt
     7441 gatcaaaaag acccaataca agctaaacag tatcgccaat tgaaatcctt attcaaatta
     7501 cttttaaaat cagaggatcg tttagattac aatacactaa cgaaaagacg caatttcaat
     7561 tggagactat tgactgaaac agaagtagtt gaccgccttc taaagatttc tcctgaactt
     7621 aaaacagctt atcgttacta ccaagacctg cttactgcgt atcaggaaaa agatccagat
     7681 acttttttcc agctgcttcg agcaatgcct ggagaagttc cattggaact gcaccatatc
     7741 aaaaaagcct ttttcaaata cgagaaaggg attcggctgg cattactgaa gaagtattcg
     7801 aatgcacgac tggaaaatct ccatac
//
LOCUS       DAFEZB010000101         7606 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFEZB010000101
VERSION     DAFEZB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.101"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(37..642)
                     /locus_tag="LP859_002554"
     CDS             complement(37..642)
                     /locus_tag="LP859_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL3030449.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(838..1302)
                     /locus_tag="LP859_002555"
     CDS             complement(838..1302)
                     /locus_tag="LP859_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3030450.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(1324..1644)
                     /locus_tag="LP859_002556"
     CDS             complement(1324..1644)
                     /locus_tag="LP859_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL3030451.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="LP859_002557"
     CDS             complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="LP859_002557"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL3030452.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPADT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            3066..3416
                     /locus_tag="LP859_002558"
     CDS             3066..3416
                     /locus_tag="LP859_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL3030453.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            3601..4554
                     /locus_tag="LP859_002559"
     CDS             3601..4554
                     /locus_tag="LP859_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3030454.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            4567..6354
                     /locus_tag="LP859_002560"
     CDS             4567..6354
                     /locus_tag="LP859_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030455.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            6341..7543
                     /locus_tag="LP859_002561"
     CDS             6341..7543
                     /locus_tag="LP859_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030456.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         2168 a         1445 c         1367 g         2626 t
ORIGIN      
        1 aaaaagaggt tgtgacaaag acttgtcaca acctctttat tttggaaata cttctccgac
       61 tacggcatcc tcaggcaata ctaagattcc ttttttttct ggagcatttg gcaaatgcaa
      121 ttcacgagca gaacaaatca ttccatagct ggctacccct cgcaaagttc ctggccagat
      181 catcattcca tctggcatca tggctcctgg ttttgcaacg acaacttttt gtccagcttc
      241 gatatttggt gcaccgcaaa caatttgcaa tactgtccca ttgtccactt ctgtctcagt
      301 aatcgataaa tggtcagagt caggatgcgg gacgcatgtt tttacatacc cgacaacgat
      361 cttcggttca tgatcagctt caagctgttc ttcgaatcct gcttctttaa tcaatcgatt
      421 caatttcgct aattcttcat ctgttaagct tacttggccg ttcccttcaa ttgttagatg
      481 atcggagata tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc
      541 aacattccat ttacgttcga cagtgttttc tgttccttta tcgtcggcta tgataaccat
      601 caaagtgtct ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc
      661 ctttgctgag atttatctta acaaaaaatt gcattcactg catgagattt ttttcatgct
      721 ttatttccga agaagcttct tctggaacac catttattcg taacaaggga ctgtgacaag
      781 acttttgtca cagtcccttt actgtttgat taaaaaggtt atacaagttc cacttgctta
      841 attactcggc tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat
      901 gacgtagctg gctacacttc ctgtcataaa gcgttcgact gcatttagtc cagactgccc
      961 aaccatgatt agatcgattg catatttttt tggcaatttg actgccatgg attcacgaac
     1021 agagccgtat gcaataattc cttctatatt tgtgtaatca acagattttc catattcttt
     1081 acattcttcg atcatttgct tggctgtttc agtttgctga tcgactagat tttgatcaat
     1141 aggagcatag cccataaaat tataaaattg ctgatcgatt acgtttgcta catatacttt
     1201 cccattatta cggcgagcca cttctaacgc ttttttgaaa gcagcaagtg cttgttcact
     1261 accgtcaata cctactaaaa tgttcttata ttcttgattc atcttgaccc ctcctaacag
     1321 atgtcagcct tttattacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc
     1381 tttattcact agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc
     1441 aaaaatgttc cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg
     1501 ccaatcagaa aagtcatttt cgatttcagg catttgcggt ttgataaaac ggcagtctcc
     1561 gcaccagccg gcagtaaaga aaaatacact ttttccagtt tctacgtagc ctgctaattc
     1621 ttctaaacta tttgggataa tcattctttt tcctctactt tctgtttatg tatttttcaa
     1681 ttaaattata cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat
     1741 ctgacaaaag gttgcttttg ccagatttta tagtttcact atcgcgttat ttcccgtata
     1801 cgatcgtgtt cactgttgat ttatctaaac ctttcaatgt ttgaatcaac atttctcgtg
     1861 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     1921 cgccaatcac tgtacttggg attccttcat tttgtgtatg agcagcacct gcatctgtac
     1981 cgccttttga aacaaagtat tgataaggga tgttgtttgt ttcagcgata tctagcaagt
     2041 actcacgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     2101 ctcctaaatg tccgaatgtg ccatttttcg tcactgtgtc atctgctgca gagcagtcta
     2161 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     2221 cttcttcttg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgtaacg
     2281 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccacgat ttgctgatga
     2341 tattcttccc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctgctggaa
     2401 gaacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatctg
     2461 ttacttctac tgatttttgt ccgcttgttc ctcgtaataa atgtggggga acggaagaag
     2521 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgttga gcagaaacaa
     2581 cgtaagggtt ccatccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     2641 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacgaggt gcatccgctt
     2701 cttcatggtg gcgcagccca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     2761 gcggttccat atgactttcc atataggcgc gaacgtcttg ttcaaaaccg cttgttcctt
     2821 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     2881 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     2941 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     3001 aaaatatgag agtgtggtaa aataaacaca aagagtagca agtgagaaga aggaggtata
     3061 tccacatgta tgaagaaagc gaattaagct attttaaagg cgggttagcc gttgggatcg
     3121 gtctaggatt attgagtggg gtagcttcaa cgatttggta tcatcgcaaa aaagtgttat
     3181 cagcagacca agtgcttgaa aatatcaaaa catctttttt aaaagaaggc gcaatcgaag
     3241 gtgcttggat ctcttttgaa aaagaaccta ttcggaaatt cgccgttcgg tcgagtgctt
     3301 atcggggtgg gctttcccgg atagaagatg gcgaaactgt ttattatgaa tttttagcag
     3361 atgctcatac aggaaccgta ttagacattt caagaaaaaa gggcgcaata gattaatacg
     3421 tcaaaaaaat gaaataacga atacgcaccc actatctaaa agagaggtat cctatccttc
     3481 ttttatagtg ggtgattttt ataaaaatag ggctatttcc tcttggaata gctagtattt
     3541 gtagttatta tacggtattc ttgaaataag attagtttta ttatataagg agagtgacaa
     3601 atggaatcac gtgaaaaaat tgccgtgctc attccttgtt ataacgaaga ggcgacaatt
     3661 tcaactgtta ttgaagattt taaaagggaa ttaccagaag cagatattta tgtctatgat
     3721 aataattcga ccgatcgaac atacgaattg gccgttcaag gcggagcaat cgtaaagaaa
     3781 gaaccacgtc aaggaaaagg gaatgtaatc cgtcaaatgt tttttgatat tgatgccgat
     3841 tattatttga tggtagatgg tgatgatact tatcctgcag aagctgcaca tggtttgctt
     3901 gagaaattac gttctggcga agcagatatg gtcattggcg atcgtctatc aaatggaacc
     3961 tattttgatg aaaataagcg tcctttccat gattttggga ataatctggt taaaaataca
     4021 attacttact tatataaaac aaaaattcgc gatgtcatga ccggctatcg aggattcaat
     4081 cgaatttttg tcaaaagctt ccctattatg agttctggtt ttcaaatcga aacagaattg
     4141 acgattcatg ctttagataa aaaattcaag ttcgttgaga ttccaatcga ttatcgcgat
     4201 cgtcctgaag gtagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     4261 atgatcatca aaatgtgcaa agattataaa cctttgcttt tctttgggat ctggaccatc
     4321 ttcttctttt tatttggatt ggtcatgggg attcctgtga ttcgtgaata tatgctgaca
     4381 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagccctt
     4441 ctttcattag taacagggtt gattttggat acggtcgtca caaatgccaa gaaagaatat
     4501 gaattgaatt tataccatgt ctaccacgaa tatcaaaata aagaaatgaa gtaggaggcc
     4561 ttttttatgc ataagaaaaa ggagaatcta tttcctactt tttttgcaac tttatttgcg
     4621 tcaggttttg ctttttttac ttcttatggt ctaggaactt tgaatccaac agtaatcatt
     4681 ggtttagcca tggttttgct acttgctttt aagcaatatg gaatagagtt gttttcaata
     4741 aaaaacaaag ttgcagctat tgcccgattg tttttttctg cagcttttgc tcttacactt
     4801 tctattggca gacagataca atttcagtat gtctcagcaa actatacaaa gacaacgatt
     4861 gctgatggaa gtatttttta ctttcttgtt tatttctcca tttttgtgta ttctttttta
     4921 tttttacaat cgatttattt atcaatgcca aaactggaaa cattttttac tgctcaaaac
     4981 acacaggctt tctcttcaag aaaatatttt ttattcttta ctgtcttttt agttctgtgt
     5041 tggcttcctt atctgttggc attgtatcct ggagtggttt tgcctgattc gttaagttcg
     5101 gtcgcccaat ctatgggtga tgttcctata agcaatcatc atccagtctt attcacctta
     5161 ctggtcaagc tatttgttca tgatttacca actgatacga tcaatcacgg tgtattttta
     5221 ttttcgattt ttcaaacatt gataacagct tcagcgataa gttattctct ttgctggctg
     5281 ttaaaaagaa acatccattt ttttccggta ctcattagtt tatgctattt tgcatttgca
     5341 ccggtttttc caatctacgc cttgaacatg caaaaagata ctttgttctc tatcgcttgc
     5401 tttttatttt ccttagcggt ttttgacttt atttttcaac gagaaaagcc aacgattctc
     5461 aatcagattt acgtaatcat ccttgcttta ctgaccacct atctaagaaa taatgggatt
     5521 tatgtagtaa ccggcacagg tttgatttgc ggcttgatta ttttgaaaca aaaaaggtat
     5581 caagcattct ttcaagtggt gctgggttat acagtgattg ctggactact catccacccg
     5641 ttgatggctc gttatgctat tcctacagaa actgctgaac aattaggtgt tcctttgcag
     5701 caaatttcta gaactattgt tatggatggt gacttgtcta agaaagataa agcattcatg
     5761 aatcagctac tcccgataaa agattatgaa gcgtattctc ctagtcttgc agactcaatc
     5821 aagtggcata aaaattttaa caatgagttt ttgaatacac ataaaaaaga atttctatca
     5881 ctatgggcaa gaaatttacc aagtaatata aaaatctata cagatgctta tattttagaa
     5941 acgttcgggt tctgggtacc tggtacaaaa aatagttacg gctttttaga tactcgtgta
     6001 aacacgaata attatggtat gaagcaagta gatttgattg aacatcttac tggattgtca
     6061 ggaatgaaaa aaatcatcga tgctcgtgat ttctttggtt ccggcacatt actgtggtta
     6121 gctatcggca gtgcactttt agcaatcttt aagaaaaaat atcggtattt actccttttt
     6181 cttcccggta tgcttgtttt tgctactatt atgattgcta ctcctgtggc ttttagttta
     6241 cggtatgtgt ttgttttagc attaggtcta cctgtttata tcctctctcc ttttttacta
     6301 gataaaagag aaaaaatgaa aacagatata ggagatactc atgaaactat ttaaaaagat
     6361 attttttgct tttgttctct tgatcgctag ctattgcgta ctactgacaa ttactttttt
     6421 cattccgcag cactctattg aatcgaatgc agaaaaatct ctacagatgg ttgaaaaaga
     6481 aggaatgtat ccttctatca atcaaggaaa tatgctaggc actcggttag ataattttac
     6541 tgatcatttg atgatacgaa aaacaaaagc agatcctgaa ttgaactcct tggagaacgc
     6601 gatgtctatg gcggattatc cccgttattg gcatggttat cagctttttt tacgaccttt
     6661 actgctaggg atgagtttgg ggagtattcg aatgatttac gcagccattc tttttctgct
     6721 gattggattg acgagctact ttttaaccaa gtgtagcgac atctatttaa cgacagcctt
     6781 gttgatttcg cttgctatag gaaatgctgc aacctttttc ttcagtatgc aattttcaaa
     6841 tatttggatt ttgactttat tgtctgtatt actattttta acaaaaccac aactctccaa
     6901 caccaaccaa caattattct tgtatttctt tgtcattggt tctttagcta acttttttga
     6961 cttgttgaca acaccggtca tctcttgggg gatacctgtg atcattattt attatgtaac
     7021 aaataagtat ttaatggata aacgcagttc attgagcaaa caagtaggca gttttgtttt
     7081 taccggtatc ttttggggat taggctatgg tttgacatgg gtggctaaat ggatcctatc
     7141 atcgatcata ttgaacaaaa acattgtaaa agatgcaatc aaccaaatcc tctttcgaac
     7201 ggaaggaaac gacaaatacc cgttgcatcg attagagatg ctgaaaagca atattagatt
     7261 gatgtatcca aaagtagcaa tattattgct attgattact tgtcttgttt ttctctattt
     7321 tgcttatcgt aaacgaaata gctggcgtcc gttccaactt ctagttttgt ttgttttagc
     7381 tgctgtaacg ccgtatattt ggtacaatat tttagctaat cactcacaga ttcattattg
     7441 gtttacgtat cgtacgcaaa tcatcacaag ttttgctata cttagtgctt tcgccttttt
     7501 gatccctcct gagaaatcaa aagacgaact aaattacttt taagaatacc gatgtactaa
     7561 ttcattttta aacataaaaa ctccagctgt cagagatagc cggagt
//
LOCUS       DAFEZB010000102         7600 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFEZB010000102
VERSION     DAFEZB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.102"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..476)
                     /locus_tag="LP859_002562"
     CDS             complement(<1..476)
                     /locus_tag="LP859_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL3030457.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYY"
     gene            complement(617..1219)
                     /locus_tag="LP859_002563"
     CDS             complement(617..1219)
                     /locus_tag="LP859_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL3030458.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1464..1670
                     /locus_tag="LP859_002564"
     CDS             1464..1670
                     /locus_tag="LP859_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030459.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1982..2701
                     /locus_tag="LP859_002565"
     CDS             1982..2701
                     /locus_tag="LP859_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL3030460.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2782..3465)
                     /locus_tag="LP859_002566"
     CDS             complement(2782..3465)
                     /locus_tag="LP859_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL3030461.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3683..5869
                     /locus_tag="LP859_002567"
     CDS             3683..5869
                     /locus_tag="LP859_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL3030462.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            6215..6625
                     /gene="spx"
                     /locus_tag="LP859_002568"
     CDS             6215..6625
                     /gene="spx"
                     /locus_tag="LP859_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL3030463.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            6639..6995
                     /locus_tag="LP859_002569"
     CDS             6639..6995
                     /locus_tag="LP859_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL3030464.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         2844 a         1237 c         1427 g         2092 t
ORIGIN      
        1 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
       61 tgaaaccaag tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg
      121 gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
      181 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
      241 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
      301 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
      361 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
      421 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
      481 tacagctccc attgattttt accaaattat agcacaattg gcgtattatt tttttaattt
      541 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
      601 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
      661 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
      721 tagaaaagta agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga
      781 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
      841 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
      901 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
      961 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
     1021 agatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
     1081 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt
     1141 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
     1201 tcacaacttt atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat
     1261 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta
     1321 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
     1381 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta
     1441 taaaaggtga tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat
     1501 atgcatttaa atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg
     1561 aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata
     1621 atctgcactg gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa
     1681 aatgttgaga gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa
     1741 tgttaaaaaa cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg
     1801 agaatactgt ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc
     1861 atgaatagga gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt
     1921 caaggctttt atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag
     1981 gatgccgata attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac
     2041 tgaaatgctt gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga
     2101 agtattgccg ctattttatt ctaatccaga aaagtatact tttttattac agattttctt
     2161 tttgaacaaa cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga
     2221 ccgctccatc tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc
     2281 tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga
     2341 aatttcgcca caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat
     2401 gttagcgaga atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact
     2461 ctattcctac tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat
     2521 atgtcccaaa atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg
     2581 agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc
     2641 ccagtcgatc cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata
     2701 atataaaaac taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt
     2761 agtttttttg attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa
     2821 atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg
     2881 ggcgccaaac agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac
     2941 gacacttaga tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc
     3001 atataagttc gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg
     3061 cccgttgatt ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt
     3121 tactaatgcg ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc
     3181 ataatataga acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag
     3241 atactttgtt agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac
     3301 acttagccat tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc
     3361 agaggcaaaa gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt
     3421 cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt
     3481 ttatgaatat ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt
     3541 cagaaaacta aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa
     3601 gaaaaaatag acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa
     3661 agatgggaag gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt
     3721 gaaacgctgg gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat
     3781 cattgctacc tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca
     3841 agtaaaagaa agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa
     3901 tcgcttcaat caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat
     3961 catagcagga gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat
     4021 gacgcaaaaa caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc
     4081 aaatgaacta aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg
     4141 aacattccat agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt
     4201 agcaatgcaa ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt
     4261 tttgcaacag ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac
     4321 gattgcttct cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca
     4381 gttagttaaa gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg
     4441 actgtatgga ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt
     4501 caatgatgta ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat
     4561 cggccaaaaa tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg
     4621 ggaaatcgtg aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga
     4681 tgatcagagt atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa
     4741 agaatatcct accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt
     4801 ccaaggagca aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat
     4861 tccagcacaa aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga
     4921 aagtcagtgg ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga
     4981 atcaatcata ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa
     5041 aacgaatatt ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa
     5101 gtctttccaa catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa
     5161 gaaaaatcag gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa
     5221 aagtgaatgc gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat
     5281 tttgtcgata agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc
     5341 gttgcgtaaa acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct
     5401 gccacgtata gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa
     5461 gcagcatgct actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa
     5521 agaattaaaa acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca
     5581 gagcattcac aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga
     5641 aggaagtttg ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa
     5701 agcagaacca gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag
     5761 agcaagaaat cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt
     5821 atcgagattt atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg
     5881 gtgtgacaga agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa
     5941 atttttgtga aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg
     6001 aaaagggagg tgtgacaaga attttgtcac acctccctta tttattttga aaatgagaat
     6061 actattatca aaatagagaa tatatagaaa aattctcatt attcaaacgt aaataatcag
     6121 ttattttgat tttttttata ttttattagc taataattgg tattttaaaa gaaattactg
     6181 tagaattgga ttataattaa aaaggagtga taaaatgata aaagtatata cttcgcctag
     6241 ctgtacttcc tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga
     6301 gaagaatatt tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac
     6361 tgaaggagga gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat
     6421 agattttaac gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt
     6481 attgagacga cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga
     6541 gatccggtca tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca
     6601 aaacaataac atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat
     6661 cagcgcactg tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt
     6721 atcaatttgt tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca
     6781 gttttgaaga agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca
     6841 ttgcgattga cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata
     6901 ccaatctcct cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg
     6961 agcagttgat ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac
     7021 tgagcatttc atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt
     7081 tttttgactt tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat
     7141 ctaggtgggg atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca
     7201 tttctgttgt tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt
     7261 tgttgacatg ttagaaatga catgataaga taactgagtt gttaaaaaag aaccacttgt
     7321 gaagcttgat tctatcgaaa aaaacttcaa aaaagttctt gacaaataac aaatgataag
     7381 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     7441 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg
     7501 aatcggggaa cactcctgat gaagtagacc tttgaaaact gaacaaagta agacaaacca
     7561 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca
//
LOCUS       DAFEZB010000103         7595 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFEZB010000103
VERSION     DAFEZB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.103"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(939..1352)
                     /locus_tag="LP859_002570"
     CDS             complement(939..1352)
                     /locus_tag="LP859_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL3030465.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="LP859_002571"
     CDS             complement(1808..2293)
                     /locus_tag="LP859_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030466.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2677)
                     /locus_tag="LP859_002572"
     CDS             complement(2432..2677)
                     /locus_tag="LP859_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL3030467.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2870..3670
                     /locus_tag="LP859_002573"
     CDS             2870..3670
                     /locus_tag="LP859_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3030468.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3689..5557
                     /locus_tag="LP859_002574"
     CDS             3689..5557
                     /locus_tag="LP859_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3030469.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5550..6041
                     /locus_tag="LP859_002575"
     CDS             5550..6041
                     /locus_tag="LP859_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL3030470.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6029..6583
                     /gene="srlA"
                     /locus_tag="LP859_002576"
     CDS             6029..6583
                     /gene="srlA"
                     /locus_tag="LP859_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL3030471.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6601..>7595
                     /locus_tag="LP859_002577"
     CDS             6601..>7595
                     /locus_tag="LP859_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL3030472.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1442 g         2233 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaagt
     2641 tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat
     2701 tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc
     2761 tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta
     2821 gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg
     2881 gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag
     2941 tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg
     3001 ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat
     3061 agaagaaggg atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa
     3121 tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga
     3181 attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat
     3241 gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc
     3301 ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc
     3361 gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt
     3421 aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc
     3481 tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag
     3541 tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta
     3601 tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac
     3661 aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt
     3721 gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac
     3781 cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc
     3841 aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga
     3901 tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat
     3961 cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc
     4021 tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat
     4081 gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg
     4141 agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac
     4201 gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa
     4261 tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat
     4321 ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat
     4381 ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca
     4441 atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat
     4501 aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg
     4561 attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc
     4621 tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat
     4681 agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc
     4741 tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt
     4801 agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac
     4861 aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat
     4921 acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc
     4981 tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac
     5041 taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt
     5101 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     5161 agaatattta aacaaattag tcgctccttt gatcagaagc tattatgtgg atgaagaatt
     5221 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     5281 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     5341 tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga
     5401 agaaatgacg gctgaagttg agaatgaatt atttgagcta tgcgatatcc tatttgaact
     5461 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5521 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5581 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5641 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5701 tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca
     5761 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5821 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5881 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5941 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     6001 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     6061 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     6121 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     6181 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     6241 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     6301 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6361 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6421 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6481 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6541 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6601 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6661 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6721 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6781 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6841 atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca
     6901 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6961 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     7021 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     7081 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     7141 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     7201 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     7261 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     7321 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7381 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7441 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7501 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7561 attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAFEZB010000104         7289 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFEZB010000104
VERSION     DAFEZB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.105"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            59..142
                     /locus_tag="LP859_002578"
     CDS             59..142
                     /locus_tag="LP859_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL3030473.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            267..1004
                     /gene="erm(B)"
                     /locus_tag="LP859_002579"
     CDS             267..1004
                     /gene="erm(B)"
                     /locus_tag="LP859_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL3030474.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1174..1434
                     /locus_tag="LP859_002580"
                     /pseudo
     CDS             1174..1434
                     /locus_tag="LP859_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1597..2262
                     /locus_tag="LP859_002581"
                     /pseudo
     CDS             1597..2262
                     /locus_tag="LP859_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2298..2543
                     /locus_tag="LP859_002582"
     CDS             2298..2543
                     /locus_tag="LP859_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3030475.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2646..3515
                     /locus_tag="LP859_002583"
     CDS             2646..3515
                     /locus_tag="LP859_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL3030476.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3496..4230
                     /locus_tag="LP859_002584"
     CDS             3496..4230
                     /locus_tag="LP859_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3030477.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4263..5171
                     /locus_tag="LP859_002585"
     CDS             4263..5171
                     /locus_tag="LP859_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBL3030478.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5168..5710
                     /gene="sat4"
                     /locus_tag="LP859_002586"
     CDS             5168..5710
                     /gene="sat4"
                     /locus_tag="LP859_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3030479.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5803..6597
                     /locus_tag="LP859_002587"
                     /note="aph(3')-IIIa"
     CDS             5803..6597
                     /locus_tag="LP859_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL3030480.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6873..>7289
                     /locus_tag="LP859_002588"
     CDS             6873..>7289
                     /locus_tag="LP859_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3030481.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADN"
BASE COUNT         2550 a         1101 c         1530 g         2108 t
ORIGIN      
        1 taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat
       61 gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa
      121 cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg
      181 actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca
      241 gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt
      301 taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata
      361 ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta
      421 aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa
      481 aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca
      541 aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa
      601 aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca
      661 agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc
      721 agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct
      781 taataaaact tacccgccat accacagatg ttccagataa atattggaag ctatatacgt
      841 actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc
      901 atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat
      961 tgtctatttt taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt
     1021 tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta
     1081 ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa
     1141 ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc
     1201 aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt
     1261 actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg
     1321 gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc
     1381 gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg
     1441 ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg
     1501 gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat
     1561 ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac
     1621 caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc
     1681 attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg
     1741 ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa
     1801 ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac
     1861 ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt
     1921 atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc
     1981 ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa
     2041 tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc
     2101 cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta
     2161 aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa
     2221 aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt
     2281 aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa
     2341 ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg
     2401 ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta
     2461 gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata
     2521 aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt
     2581 gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat
     2641 gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata
     2701 tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata
     2761 tagatatcgg catctattac aattcagaat catttgacat aaatactatt aatcaattcg
     2821 ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg
     2881 attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac
     2941 gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca
     3001 attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga
     3061 ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa
     3121 cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct
     3181 ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg
     3241 tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta
     3301 tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata
     3361 ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca
     3421 tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa
     3481 acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac
     3541 agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa
     3601 aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg
     3661 cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat
     3721 aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt
     3781 gccatagaag atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg
     3841 tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct
     3901 ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac
     3961 tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag
     4021 ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc
     4081 acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag
     4141 ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg
     4201 ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt
     4261 aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac
     4321 gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat
     4381 ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg
     4441 actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat
     4501 tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg
     4561 atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa
     4621 aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt
     4681 atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg
     4741 taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt
     4801 ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa
     4861 caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg
     4921 atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag
     4981 cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc
     5041 attatgccta tccggagtat gataggaata taacaaaata taccagggac atgtataaaa
     5101 aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc
     5161 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
     5221 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
     5281 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
     5341 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atctttacta
     5401 ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta
     5461 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
     5521 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
     5581 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
     5641 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     5701 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     5761 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     5821 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     5881 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     5941 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     6001 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     6061 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     6121 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     6181 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     6241 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     6301 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     6361 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     6421 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     6481 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     6541 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     6601 ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg
     6661 aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga
     6721 tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt
     6781 ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac
     6841 tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta
     6901 ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta
     6961 atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa
     7021 ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa
     7081 tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg
     7141 gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga
     7201 agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat
     7261 ctggacagaa aaatacattg gcggataac
//
LOCUS       DAFEZB010000105         7087 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFEZB010000105
VERSION     DAFEZB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.106"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(82..354)
                     /locus_tag="LP859_002589"
                     /pseudo
     CDS             complement(82..354)
                     /locus_tag="LP859_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            536..871
                     /locus_tag="LP859_002590"
                     /pseudo
     CDS             536..871
                     /locus_tag="LP859_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1036..1263
                     /locus_tag="LP859_002591"
                     /pseudo
     CDS             1036..1263
                     /locus_tag="LP859_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1389..2360
                     /locus_tag="LP859_002592"
     CDS             1389..2360
                     /locus_tag="LP859_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3030482.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2501..3814
                     /locus_tag="LP859_002593"
     CDS             2501..3814
                     /locus_tag="LP859_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL3030483.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIISLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3857..4723
                     /locus_tag="LP859_002594"
     CDS             3857..4723
                     /locus_tag="LP859_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3030484.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4720..5568
                     /locus_tag="LP859_002595"
     CDS             4720..5568
                     /locus_tag="LP859_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3030485.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5582..7054
                     /locus_tag="LP859_002596"
     CDS             5582..7054
                     /locus_tag="LP859_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL3030486.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT         2217 a         1197 c         1467 g         2206 t
ORIGIN      
        1 tccatgttcc ccactcctca agtgttttct ttagtatacg cctataggag gaaaaacgaa
       61 tacaatttaa ctcaatttat atagtatttt taatgctact tttttctgtt tcataaaggt
      121 aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac
      181 acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcact ggatatcttt
      241 accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg
      301 tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac
      361 tccttttaaa aaatgatata atttacatgt aaaatttact tgtagcttta ctgttatcat
      421 agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa
      481 tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca
      541 gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg
      601 caaactttat caaacttatt acgacggtat ggagaaacag ctcaacgcat taggattagt
      661 aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa
      721 aacggaaggg ttcgactgtt ccgaagaagt gatacgcaag caatctccat taattagtga
      781 tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat
      841 actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc
      901 attttcgcac ggatgttcca gatactattt ttgaatgtat aaaactgaaa acacaagaat
      961 cctattaaat caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta
     1021 tgttccatgt accccaatct tgaaaaatta attaaagact tatataaagc cattgatgac
     1081 ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt
     1141 taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa
     1201 ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa
     1261 aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta
     1321 atttggaatc gtttccttct tttggtataa taatacctga aattcttttg agaggagttg
     1381 ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta
     1441 cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg
     1501 ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata
     1561 gcagcaatac aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg
     1621 cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga
     1681 acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg
     1741 gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg
     1801 tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag
     1861 gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg
     1921 ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt
     1981 gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttgg
     2041 aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca
     2101 atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta
     2161 aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac
     2221 tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg
     2281 cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg
     2341 gagaaacgac taaagagtaa aaaaccggtt tataacgatt ttttttgaga taggttgaca
     2401 gaaagtggtt tcaatgatat cttaaaacca attaattgga aacgttacca attaattgga
     2461 aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg atgaaaagga gaatgttagc
     2521 aattgcagca attttagcat taacagctgg aggacttgca ggatgtggca gtaaggagaa
     2581 aagttcaggc agcagtgatt caggagaaat taagatttgg gttcagtttt ctgatgaaac
     2641 gcctgaagga aaagcttggg cagagattgt gaacaatttt aatgatgaat acgagggtga
     2701 atataaagct gttactgagt atattcctcg tagcggaagc ggcggtggat atgaggataa
     2761 agtaaatgct gcaattacaa ccaataccct tcctgatgtt attacgttag atggaccgaa
     2821 tactgcagct tatgctcatt caggagtcat tgctccatta gatgattatt tgaaagatgc
     2881 ggacatggat gatgtattag acagcattat tcagcaagga acctacgatg gcaaaatgta
     2941 tgcgtttggt ttctcagagt ccagtgtggg tgtctattac aataagtcgt tgttcaaaga
     3001 tgctggaatt acagaagatc aattgccgac gttagaagat ccttggactt ggacggaatt
     3061 tcaagcaatt tgccaaaagc tgactgataa atacaaaacg cctgcaattg atatgcaaat
     3121 ggcgtcggga gacgaaatgc tgacctacgc atatacacca tttgtatggt cgaatggcgg
     3181 cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat tttaacgata aaaaatccgt
     3241 tgaagcgttg gaatttattc aagaattggt tgaaaaaggt tacaccacaa tcagtcctat
     3301 tgaaaaagga tttgaaactg gcaaatatgc catgctgctg agcggttctt ggacaatggc
     3361 agatttagag gaaaactatc aagagattga ttatggtatt ttgccttacc ccgtttcaga
     3421 tgcaactggg gagttagttt caccaaccgg cagctggcag ttagcaatga ctgagaaaac
     3481 agagaaaaag gatgcttctg cggcctttat taagtttgca accagtacag aatctagtcg
     3541 tattattagt ttagggaaca gtgttctacc tatcagaaat tcaacagttg atttaattaa
     3601 agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa aaccaaattt ctggacatgc
     3661 ccgcccagtg gttgtagctt atccgcaagt atctagagca ttccaacaag cagttcaaga
     3721 tattagctac ttcgaggaaa acccagatgt acaaaaagta gcagatagtc gcgctgctga
     3781 aatgcaacag gcaattgata cttcactaaa atagaagttt ctaaaggggg ccaatcaagt
     3841 gttaaaaaaa ccgacaatga aagacaattt gataggttac ggctttctag cgccagcttt
     3901 gctgctgctg acgatttttc tgatcatacc cgtaatcatg gtaatttatt atgcctttac
     3961 tgattattat ttgttaacgc cagatgctag acaatttatc ggcttagaaa attttaagcg
     4021 actgttcgct gatccaattt ttgtaaaaag tatttggaac acggctaaat ttgttgtttg
     4081 gattattccg gtccaattag gggcagcttt aggaatggca cttattatca acaaaaatcg
     4141 taaaggaaat atctttttta aagtagcctt ttttgcgcca gtcgtgatgt ccttagtcgt
     4201 tatttcgatt ttatggttgt acttgttaaa tcctaatgaa ggtctggtta atacgctatt
     4261 tgcaaagatt ggaattgctt cgcagccttt tttgactagt cccaaacaag caatgtatac
     4321 gcttgttttc gtatcggcat ggcaaggtgc aggatatcag atgctgctgt ttttaggggg
     4381 aatgcaaaat attccgcaag acgtttatga ggcagccgaa attgatggtt tttctaagtt
     4441 tcagcaattt cgctatatca ccatgccatt attaaagccc acggcgattt ttattttact
     4501 taccacattg atttcggcct ttaaactaat cattcagcca atggtaatga ctcaaggtgg
     4561 accaatgaat tctactatga caatggttta ttacatttac caaactggat ttacagaccg
     4621 tatggtaggc tactccagct ccattgcgtt aatctttacc accatgattg gtttaattac
     4681 aattgctcaa agaaaattgg taaaggagga tgatccagca tgaccaagaa atttggaccg
     4741 ctaatgatcg tagaatattg tttgctgttc cttttagcgg cattattcat tttcccaatg
     4801 ctgtggatgg tagtttcctc tatgaagcct gaagcagatg tctatcaaaa tttagcttcc
     4861 ttcaaagctt ttttacctag tttgaatcca gctaattggt ttaaaactta tcaagaagtt
     4921 atttcccggt tcagtgtggg aacttattta ttaaacagta ttttttatgg tttaaccttt
     4981 gcctgtgggt caatcattgt aaactctttg gcgggttttg cctttgcgaa ggttaatttt
     5041 tcaggcaaga aaattatctt tggagctttg ttagctctgc tgattgtccc gatggagacg
     5101 gttttaattc cgcaatttac catcgttaat tcactaggct tagtgaatac tcgcttggca
     5161 gtgattttac cagcgatggc cagcgcgttt aatatctatt tgtttagaaa tttctttatc
     5221 gcgattccag aagaaatcat tgaatcagcg aaattagatg gtgcaaatat tgttaaaatt
     5281 ttctttcaca tcatgctgcc aatgtctaaa ccagccatag ccacagtagg tgtcttatcc
     5341 tttatctcca gctggaatga ttatatttgg ccgttaatgg tcttaactga cagcagcaaa
     5401 ttttcaatgc aagtggcaat tactacgatc aatactacac agccagttta tatcaatcaa
     5461 gtgatggctg ttttaaccat tgcaacgatt ccactgatta ttgtttatat cgttgcacaa
     5521 aaatatattc tgcagggact tggcggttct ggaacgggta taaaatagga gtgaatagtg
     5581 aatgttgcaa aaacttttaa aggccaatga gtttattgag aaaaatgctc aaacagtcaa
     5641 tcaagaatac cggaataaat ttcatttgat ggctccgatt ggctggatca atgatccaaa
     5701 tggttttgtt tattatcaag aggagtatca cttgttttat caatattatc cttacgacag
     5761 catctgggga ccaatgcatt ggggccatgc taaaagcaag gatctaattc actgggagca
     5821 tttgccagta gcactggcac caggtgaaag ctatgacaag gaaggctgtt tttccggcag
     5881 tgccatagaa aaggatggca agctgtattt gatttataca ggacacaatg ttgtggaagg
     5941 gcaagttcga caagttcagt gtttggcagt ttcagaagat ggcattcact ttgaaaaata
     6001 tgccggaaat ccaatcatcg cagaagatca cttagcttct gttgcaacga ccgaagattt
     6061 ccgcgatccc aaagtgtttc agcgaaaaga agactatttt actgtagtcg ctactaaaac
     6121 tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa gatttactaa cttgggagtt
     6181 ttattccgta ttgctggagg gaactccaga acaaggcatt gtatgggaat gcccagactt
     6241 gtttgaacta gatggcaaag aagtcttgat tctttctcct attcaaatga ctccacaagg
     6301 caatgcttac caaaatacga actcaactgt tgctttcatt ggtactgtcg attggcaaac
     6361 aggcaaattt ctagtagaga attttcatga aatcgatggc ggtttagatt tttatgcccc
     6421 ccagacctgt gaaaacgaag caggccaaag aataatgact gcttggatgc aaatgtggca
     6481 gcgtaaaata cctacgaatg atctgggcca tggttggtca ggttccatga ccttgcctag
     6541 agagctgagg gtggctgata atcaattgtt gcaacggcca gtttcatcaa tttatcaagt
     6601 aattgatccg caggaaagga ttgaatgggc gaagactgaa gagaatcgat tcttactgaa
     6661 ggacttagta tatgaccagc actatttacg cttggaaatc aatttaaaac aggcaacgaa
     6721 actagaactt cactatgcga agggacccca ccatacatta ctgcttaact atgatgttga
     6781 acgggagtgc tttactgctt ccagggaaca catgccgtat gatttatcag gagctgaaaa
     6841 ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa aataattgtc taaccatcga
     6901 aattttccgt gatacttcag ctattgagct ttttctaaat gatcgtctga ctatgagtac
     6961 aactttttat gagacagaaa aaggacagga ttttcttttg ctggcagaca gtgaagtaac
     7021 cttttcattg gaaagcggct taattaatct atagaaggga gagctcagtg atgatttatg
     7081 gttcaat
//
LOCUS       DAFEZB010000106         6643 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFEZB010000106
VERSION     DAFEZB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.107"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(1..859)
                     /locus_tag="LP859_002597"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LP859_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LP859_002598"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LP859_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL3030487.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LP859_002599"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LP859_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LP859_002600"
     CDS             complement(2143..2373)
                     /locus_tag="LP859_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBL3030488.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LP859_002601"
     CDS             complement(2603..3421)
                     /locus_tag="LP859_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3030489.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LP859_002602"
     CDS             3582..4271
                     /locus_tag="LP859_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL3030490.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LP859_002603"
     CDS             4285..5787
                     /locus_tag="LP859_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3030491.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LP859_002604"
     CDS             5800..6276
                     /locus_tag="LP859_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3030492.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LP859_002605"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LP859_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAFEZB010000107         6465 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFEZB010000107
VERSION     DAFEZB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.108"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(109..564)
                     /locus_tag="LP859_002606"
     CDS             complement(109..564)
                     /locus_tag="LP859_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030493.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="LP859_002607"
     CDS             complement(718..1926)
                     /locus_tag="LP859_002607"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL3030494.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="LP859_002608"
     CDS             complement(2038..2370)
                     /locus_tag="LP859_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL3030495.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="LP859_002609"
     CDS             2518..3393
                     /locus_tag="LP859_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3030496.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="LP859_002610"
     CDS             complement(3475..4056)
                     /locus_tag="LP859_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL3030497.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="LP859_002611"
     CDS             complement(4062..5321)
                     /locus_tag="LP859_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL3030498.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="LP859_002612"
     CDS             complement(5323..6369)
                     /locus_tag="LP859_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030499.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1802 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataa
//
LOCUS       DAFEZB010000108         6410 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFEZB010000108
VERSION     DAFEZB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.110"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            75..1379
                     /locus_tag="LP859_002613"
     CDS             75..1379
                     /locus_tag="LP859_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL3030500.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1471..1836)
                     /locus_tag="LP859_002614"
     CDS             complement(1471..1836)
                     /locus_tag="LP859_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL3030501.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="LP859_002615"
     CDS             complement(1833..2198)
                     /locus_tag="LP859_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL3030502.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2390..2638)
                     /locus_tag="LP859_002616"
     CDS             complement(2390..2638)
                     /locus_tag="LP859_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030503.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2637..2765
                     /locus_tag="LP859_002617"
     CDS             2637..2765
                     /locus_tag="LP859_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL3030504.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2822..4516
                     /locus_tag="LP859_002618"
     CDS             2822..4516
                     /locus_tag="LP859_002618"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL3030505.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4520..4639
                     /locus_tag="LP859_002619"
                     /pseudo
     CDS             4520..4639
                     /locus_tag="LP859_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5167..6300
                     /locus_tag="LP859_002620"
     CDS             5167..6300
                     /locus_tag="LP859_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL3030506.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         2121 a         1222 c         1169 g         1898 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatt gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 ggactgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2281 tctaatgata acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat
     2341 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2401 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2461 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2521 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2581 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2641 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2701 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2761 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2821 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2881 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     2941 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3001 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3061 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3121 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3181 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3241 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3301 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3361 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3421 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3481 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3541 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3601 agatgaaatc actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca
     3661 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3721 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3781 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3841 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3901 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     3961 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca
     4021 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4081 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4141 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4201 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4261 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4321 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4381 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc
     4441 acgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4501 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4561 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4621 agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4681 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat
     4741 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg
     4801 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4861 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4921 cattttttca acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc
     4981 aatgattacc attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga
     5041 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5101 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5161 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5221 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5281 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5341 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5401 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5461 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5521 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5581 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5641 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5701 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5761 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5821 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5881 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     5941 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct
     6001 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6061 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6121 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa
     6181 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6241 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6301 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6361 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg
//
LOCUS       DAFEZB010000109         6338 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFEZB010000109
VERSION     DAFEZB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.111"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            290..1135
                     /locus_tag="LP859_002621"
     CDS             290..1135
                     /locus_tag="LP859_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3030507.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="LP859_002622"
     CDS             1416..2636
                     /locus_tag="LP859_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL3030508.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="LP859_002623"
     CDS             2633..5353
                     /locus_tag="LP859_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3030509.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="LP859_002624"
     CDS             5337..6281
                     /locus_tag="LP859_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3030510.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          982 c         1223 g         1770 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttt
//
LOCUS       DAFEZB010000110         6132 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFEZB010000110
VERSION     DAFEZB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.112"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(63..1268)
                     /locus_tag="LP859_002625"
     CDS             complement(63..1268)
                     /locus_tag="LP859_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3030511.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="LP859_002626"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="LP859_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="LP859_002627"
     CDS             complement(1447..2832)
                     /locus_tag="LP859_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL3030512.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="LP859_002628"
     CDS             complement(2861..3247)
                     /locus_tag="LP859_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3030513.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="LP859_002629"
     CDS             complement(3263..3577)
                     /locus_tag="LP859_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3030514.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="LP859_002630"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="LP859_002630"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL3030515.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="LP859_002631"
     CDS             4837..6126
                     /locus_tag="LP859_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL3030516.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         1774 a         1243 c         1154 g         1961 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aa
//
LOCUS       DAFEZB010000111         6059 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFEZB010000111
VERSION     DAFEZB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.113"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            825..1559
                     /locus_tag="LP859_002632"
     CDS             825..1559
                     /locus_tag="LP859_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3030517.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1575..2744
                     /locus_tag="LP859_002633"
     CDS             1575..2744
                     /locus_tag="LP859_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3030518.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2760..3755
                     /gene="lacD"
                     /locus_tag="LP859_002634"
     CDS             2760..3755
                     /gene="lacD"
                     /locus_tag="LP859_002634"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL3030519.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3834..4760
                     /locus_tag="LP859_002635"
     CDS             3834..4760
                     /locus_tag="LP859_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL3030520.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4779..6029
                     /locus_tag="LP859_002636"
     CDS             4779..6029
                     /locus_tag="LP859_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3030521.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          996 c         1243 g         1795 t
ORIGIN      
        1 cagcgaatcc ttgataatga gctcggatgt gctaacgcta gcgaggcagt ctaactttaa
       61 ctgttagctc ctgccgaaga tcgtcacatc cgaggaggct cattgtcaag gacaatcgga
      121 atagcatacg gaaagaagtt ctgcacctta taaaatttgt caaaaaaact gttgccattc
      181 caaagtttga aaaatgctat ttcgaggtag aaggatatta tataagcacg gttgactaaa
      241 cgaagtgata ataaaaaaag ttattcaaga ataagaaaaa ttatgatacg aattcagata
      301 aactggagaa tacagagacc tttaaaaggg ataagaaaca acataaattc ctcttaagag
      361 agaacgaaag gacaaagcca gcaacttgat acacgagcta gtattagagg aatttattct
      421 catagactcc cttttgaata cgtttcaact ttaattttca tgttcataaa tgatttgaaa
      481 ccaaagcaaa tgcgttgcac ttttcctccg tcttccgaaa atcgttcaat tcactggtaa
      541 gtagtcattt gatagtttga ggcaaattgt tgattgatga agggtaggag ctaaaaaaag
      601 ataccagttg aagagagaaa aagaaaaaaa tagagggtgt tcttgaatga aaaaagttgg
      661 aaaattattt ttcatttcct tttattccta ttaaagtcag tgatgtcaat catattaaca
      721 agactaaaaa tgaaaataaa aaaaagattg ttcttagtat tgactcataa aaaataccac
      781 tactatactt acattgtggt tatagccagt taggagagac atcgatgcaa aatacctttc
      841 aatcaaaatc actttattat cagctggtag aaatattgaa acaacgaatc gaaaacagta
      901 tggtcgctca tgacaagctg ccatcagaaa gagagttgac cagtttttat ggcgttagtc
      961 gcacgacggt gcgactagca ttgcaagaac tggaaaaaat gggctacatt tatcgacaac
     1021 atggaaaagg aacctttgtt tcggaattaa ggacaaatgc gactgatcta gctggcgcat
     1081 atagttttac cgaacaaatg cgggcaatgg gcaaacatcc tgaaaccaag atattggcct
     1141 tcaagaagat tgaagcaact aaatatttag cgagtcatct gaatgttcaa ctaggtgagc
     1201 ctttatttca attagaccga ttgcgcttag ctgatgattt gccaatgatg atcgagaaat
     1261 cctatttgcc agtgaagcta tttatgtccc tttctcaaga aattctattt ctgaaacctt
     1321 tatataattt gttttcagag aatttcaatc aaatcgtacg gttggctgac gaggaatttt
     1381 atgccagcat tgtccagaaa gaagatagtg agttgctaaa tattccagaa ggatcaccag
     1441 tgctccattt agttcgctca acctataatc tcgagaacga agtgattgaa tttacattaa
     1501 gtgtagcgcg ggctgataaa tttcattata agatcaggca tctgcgttcc actaactgaa
     1561 ttggaggaaa gaaaatgttt atgttctcaa aagaagaact acgaaagcta ggagcagaaa
     1621 atactacccg agaaattcga cagcaaccgg aactgtggca tgagacgttt gaccgttatc
     1681 aagaaacagt tgcagattta gatcgtttgt ttacggatgc gaaacaacat gctggagagc
     1741 gaccaatccg agtcattttt acaggggccg gaacctcgca atatgtggga gatacactaa
     1801 ttccgtttct tactagtcaa ggaaatacga accagtttat ttttcaaagt attggttcga
     1861 ccgatatcgt tgctacgcca aaagaatacc tgattcctga tgagacaacg atcttggtct
     1921 catttgcccg tagtggaaat agtccagaaa gtctagccgc agttaagatg gccaatcagc
     1981 tggtcaacaa gatctatcat atcgtgatca cctgtgcacc agacggatta ttggctacac
     2041 aaatgcctac aaaaaaagaa aatctagttt tactaatgcc aaacaaagcc aatgacgctg
     2101 gttttgctat gacaagcagt ttcagctgta tgacgctgac tgcgctactt atttttgatc
     2161 aagcaagcaa tcagcaaaaa gaaagatatg ttcatcaatt aagccgacta ggagaagaag
     2221 tcatccaacg agaatcagag atccaagggt atctggcatc agactttcat cgcatcattt
     2281 acttaggttc gggaagctta ggcggattga ctagagaagc gcaattaaaa gttctcgagt
     2341 taactgctgg aaaaatcgca accgtatttg atacatcttt aggattcaga catggtccca
     2401 aatcttttgt caatgagcaa acattggttt tcctgtttgt cagcaatgac tcatatacgc
     2461 agaaatacga tgtagacatg ctggaagaaa tcaaaaatga cggtattgcc caacgcgttg
     2521 ttggaattgg tcaaaagggt tcattgttta gtggtgattc ctttgaatta gttgcagatt
     2581 cgttactgcc ggatggctat cttgctttag cagatatcat ggtagcccaa acgatcgcat
     2641 tactttgttc ggtgaaggtt ggtaatacac cagatacacc atcgccaagt ggaacagtca
     2701 atcgtgtggt caaaggcgta acgatttatc catatgaaaa ataggaaaga gtgtgaagca
     2761 tgttagaatg gtcggaaaat aaaagacaag caatggaacg tctatcgacg ccagaaggca
     2821 ttattaccgc tttggcgatc gatcagcgag gcgcgctgaa gaaaatgatg tcggctttag
     2881 ggatggaagc gacaaaggaa aaaattgagc agtttaaaac actaatttcc caagaattga
     2941 ctccttatgc ctcagcaatt ttattggatc ctgaatacgg actgcctgct gcacagaagc
     3001 gtgcagcagc gagcggtctg ctgttggcat atgaaaaaac tggttatgat caaaccgtac
     3061 ccggccgctt gccggatctg ctgtcggact gttcagttct gcgattgaaa gagttaggtg
     3121 cggatgcggt gaaattttta ctgtattacg attatgaaga agcagaggaa atcaatcggc
     3181 aaaagcaagt ttttgttgaa cgaattggaa gcgaatgcat ggctgaagga ctgccatttt
     3241 atttagagtt attgtcatat gatgctaaaa atgtagatac gaaatcgctt tcctatgcca
     3301 aagcaaaacc gcgcaaagtg atcgaaatga tgaaagaatt ctcgaaaacc cagtataaag
     3361 tagatgtctt aaaggttgaa gtaccagtaa acatgtcatt tgttgaagga aatgcaacag
     3421 gggagatcgc ctatacacgt caagaagctg ccgcttattt taaagaacaa agcgatgcaa
     3481 cagatttgcc gtatatcttc ttaagtgctg gggtatcagc ggcattattc atggagacat
     3541 tgcgttttgc caaacatgct ggggcacgat tcaacggtgt actctgtggc agagcaactt
     3601 ggaaagatgc agtcgctcct ttcgttgaaa aaggagaagc ggcaacttta gaatggctga
     3661 cagaacaagg gacacaaaac attcgtcaat tgaatgaagt gataagagaa actgccatcc
     3721 catggtatga gaaagtaagt gaaagcactt gttaatgttt ctaatatatt agtagtgata
     3781 agagatatgt gtgattataa tttatcatta ttgaaactgg gaggatattt tatatgaata
     3841 aaagattgtt agattattca gcttcagatt ttttgaaagc taagccgatg gaattaaagc
     3901 aggcaatttt ggcttctgaa ggtagaacaa ttatggcaga aaatgtgcca agtagctgtc
     3961 cttacatggg aaaatcagtg acaaatgcag aattggagag agctgctgga gctgatttaa
     4021 ttttgtttaa tgcactagat ctttttaatc ctcaaatttc aggtgtacct ttaactctaa
     4081 aagagaatcc aataatctgg attaaaaaag ctttaggaag agctattggt gtaaatttag
     4141 aacctgtaga caaagaagtt ttgatgagtg aaaatcgaac tgaaatttca agtggtagaa
     4201 tcgtttctcc tgagacttta aaaaaggcaa atgaattagg ctttaatttt atttgtttaa
     4261 ctggaaatcc aggaacagga gtatctaata gaactattga gaaagcaata acattatcca
     4321 aatcaatttt taatggatta attatcgctg gaaaaatgca tggtgctggt gttgatgaac
     4381 caattatgaa cttagatata gcaaaaagat ttattaaaac aggtgtagat attttattag
     4441 ttcctgctcc atacactgtt ccgtatttca tggaaagtga tttgcgagga atcgttgatt
     4501 atataaaaga ttttaataga aataaatcta ttgaggaaaa agttctagta ctaacggcta
     4561 atggaacaag ccaagactct tctgaccaag aaactattaa aaaaattgca ttagcatcaa
     4621 aagcatgtgg agctgatatt caacacatag gggattcttt aaatggtatt tgtcttccag
     4681 aaaatatttt tgctttgggt caagcaattc ggggatatcg tcatcaaatt attatgttag
     4741 gaaaatctaa ttatcgttaa ttaaggagga aatgaaagat gatcgacaaa gttcaaaaaa
     4801 tagctgcaaa aattagtaat caaagacatc tactagcttt aagagacagc atggctgcag
     4861 ttatgccatt aatcattatt ggttcctttt ttatgttatt agcaaatttc ccgatagaag
     4921 gatttacaaa atggttatct gaaattggag tacaatcctt tttgaacaaa gcatcagatt
     4981 caacatttgg tattattgga ttagctgtta cctactcttt gggacacaat ctagcaaaga
     5041 cttatggtgt agactcatta tcagcaggat tattatcttt gagtggatat gttttactta
     5101 cacctttatt ggatgcagat ggttctatag gatttccaac aaaatacttt ggtacctcgg
     5161 gtctttttgt tggtattatt gtagcacttg tatctacaga aattttccgg tggtttgtac
     5221 ttaaaaatat tacaattaaa atgccggcaa gcgttccacc aaatgtaagc cgagctttcg
     5281 ttgctattat tcctggcttt tttgtcgttc tactatggtt tattgttgtt gtaatttgtt
     5341 ataaacttgg aattgaaaat gttcatgctc ttattgcaga tacactcgca aaaccactat
     5401 ctttactgac gaaaacttta ccaggaatta tattggttat acttattcaa tgtttctttt
     5461 ggatgtttgg tattcatggt gcacaggtaa cggggccaat aattgaaccg ttattattgc
     5521 aaaactctga tgtaaataga attgcttatc aagctggaaa agaacttcct aacattataa
     5581 cttatgaatt cttatataac tttgtttttt ctggtggtgc aggttgttta tttgcacttg
     5641 ccattttaac atttttcttt tcaaagagtc aggaaaataa aactttaggt aagttatcaa
     5701 tcgctccagt aagttttcaa gtagcagagc cccttctatt tgggtttcca actatattaa
     5761 atataaaaat gttaattcca tttgtaactg caccagtagt aacaacatta attacttatt
     5821 tttcaatgag tatgggatta gttgcaaaac ctgtaggagc aacaatccca tggacaactc
     5881 caccgataat tgccggattt ttagcttctg gtggacgaat aagtggtgca gtgattcaag
     5941 taataacaat tgcaataaat gttttgatat actatccatt ctttaaatta gatgataatg
     6001 ccaagctaaa aagcgaaaaa aatgattaga actacctata tgtttctaac tgtagttaa
//
LOCUS       DAFEZB010000112         6038 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFEZB010000112
VERSION     DAFEZB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.114"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..335)
                     /locus_tag="LP859_002637"
     CDS             complement(<1..335)
                     /locus_tag="LP859_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3030522.1"
                     /translation="MHVRCGAGENLEITSKGYLSLFTSDFLAGLGFGATQMLGELEGI
                     YFGYNVDTGRNVYLKPALASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFG
                     GQAVIVDPKG"
     gene            complement(375..2141)
                     /gene="ltrA"
                     /locus_tag="LP859_002638"
     CDS             complement(375..2141)
                     /gene="ltrA"
                     /locus_tag="LP859_002638"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3030523.1"
                     /translation="MQETFDWLYERSSKQATKGIDLYKIIMSEENILLAYRIIKSNTG
                     SKTSGIDNQTISDFKFQNKQKFVNDIRKTIENYQPQPVRRVNIPKSNGKTRPLGIPTM
                     KDRLIQQMFKQVLEPICEAKFFKHSYGFRPNRSTEHAIARCNFIAHKCKCHHVVDVDI
                     KGFFDNVHHGKLIKQLYTIGIKDRRVLAIISKMLKVTVAGQGTSTSGVPQGGVLSTLL
                     SNVVLNDLDWWIANQWETFQTKRKYSSVDNMHHALVKSKLKKMHIVRYCDDFKIFTTN
                     HKHAVKIYHAVKGYIKQRLNLDISPDKSKITNLRRNYSEFLGFELKVIKQKKQQYVTV
                     SRVSKKSKSRIKKEIREKIKAIQKSPTRKTVNQYNQYVRGIQNYYQKATLINHDFSSI
                     YYSCLPALYNRLKPFGKYEIPRSPPITYKKFYPLTKRTFCVRKNYLFPLDSVKWKRLE
                     FFKPHINNYTQDGRLAKLRKLKPSVYSELVTLSTMETDESLEYLDNRLSRYSMQNGKC
                     SVTGIFLRTQDVHCHHIIPRKFGGTDEFDNLVIIHKWVHRLIHAKTKETIKEYLQLLQ
                     LTNKQMKKLNKYRLECNLTEFH"
     gene            complement(2885..4114)
                     /locus_tag="LP859_002639"
                     /pseudo
     CDS             complement(2885..4114)
                     /locus_tag="LP859_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            complement(4101..4490)
                     /locus_tag="LP859_002640"
     CDS             complement(4101..4490)
                     /locus_tag="LP859_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3030524.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHMPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(4573..5298)
                     /locus_tag="LP859_002641"
     CDS             complement(4573..5298)
                     /locus_tag="LP859_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030525.1"
                     /translation="MLEVMIIMNFDFDLKSFIDIFVPVLTSFAVCFITLFFTNKQHKK
                     AMKQQERQHEESLDLSKQQYADEKQRNEEQDRLSYYPYLKLIPKISEHRFAGKMVKSD
                     ENDVFKLPFELINEGLGMAFSIDFVYLEETINTNDHEVNLLSLLSAQKHIDDSLDALG
                     VSAPIDTDILRVGKKTEFELYWMKYNNEVMSIPKYSVSKWEIKIRFSDFQDRIYQQRY
                     TFITSAGYQEIHRLWSDKPQLID"
     gene            complement(5347..5850)
                     /locus_tag="LP859_002642"
     CDS             complement(5347..5850)
                     /locus_tag="LP859_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3030526.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFSPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPIGEVALEIQHAFFNSFEEMVEH
                     VDDIICYPDCNDMSDVAYYLIEETGAFGEVPTHLQNYIDYEAYGRDLELEGNYLVTNR
                     GTFEFIG"
     gene            complement(5862..>6038)
                     /locus_tag="LP859_002643"
     CDS             complement(5862..>6038)
                     /locus_tag="LP859_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030527.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
BASE COUNT         1767 a         1209 c          965 g         2097 t
ORIGIN      
        1 cctttgggat caacaatcac cgcttgaccg ccaaacagga cagcatagta aactaaaaga
       61 ttattactaa aggatttccc accaccaagt gaaccaagaa aagcggcggc cagtgcattg
      121 gtgacggaac ctttgacccc ttgtgaagcc aatgccggtt taagataaac attgcgtcca
      181 gtatccacat tgtagccaaa ataaattccc tccaattctc ctaacatttg ggttgcccca
      241 aagccaagcc cagcaagaaa atcagaagta aatagcgata ggtaaccctt tgaagttatc
      301 tctaggtttt cccccgctcc acaccgtacg tgcaactttc atcgcatacg gcgttccaac
      361 ttgttcgtat ttctttagtg aaattcagtt aaattacatt ctaaacgata tttgtttaac
      421 ttcttcattt gcttatttgt tagttgaaga agttgtagat actctttaat cgtttctttt
      481 gttttggcat ggatgagtct gtgtacccat ttatggataa taactaggtt atcaaactca
      541 tctgtacccc caaatttacg aggaataata tgatgacaat gaacatcttg cgttcttaga
      601 aaaatccctg taacggaaca ttttccattt tgcatagaat atcttgacag tcgattgtct
      661 aagtattcaa gactttcatc tgtttccatc gtactaaggg taaccaattc agaataaacc
      721 gaaggtttta atttcctcag tttagccaat cgtccatctt gcgtatagtt gttgatgtga
      781 ggtttgaaaa attctaacct tttccatttc acgctatcta atggaaataa ataattcttc
      841 ctaacacaaa acgttctttt tgtgagagga taaaactttt tatacgtaat gggtgggctt
      901 ctagggattt catattttcc gaatggtttt aaacgattat atagtgctgg taagcaggaa
      961 tagtagatag aagaaaagtc atggttaatc aaggtggctt tttgataata attctgaata
     1021 cctcgtacat actggttgta ttggttaaca gtttttctag taggagattt ctgaattgct
     1081 tttatctttt ctctgatttc cttcttaatt ctactttttg atttctttga gactctcgaa
     1141 acagtaacat attgttgttt cttttgtttt atgaccttta gctcgaatcc tagaaattct
     1201 gaataatttc ttctaagatt tgtaattttt gatttgtctg gtgaaatatc taggttcaac
     1261 cgttgtttaa tatatccctt gaccgcatga tagattttta ctgcatgttt atgatttgtt
     1321 gtaaagattt taaaatcatc acaataacgt acgatgtgca ttttctttaa tttcgacttt
     1381 accaaagcat ggtgcatatt atctacactg ctatattttc tttttgtttg aaatgtttcc
     1441 cattgattgg caatccacca atctaagtca tttagaacaa cattcgataa taatgtagat
     1501 aacacgccac cttgtgggac gccagaagtg gaagtgcctt gtcctgctac tgtcactttt
     1561 aacattttag agattattgc taatacgcgt cgatctttta taccgatggt ataaagctgt
     1621 ttgattaact ttccatgatg gacgttatca aagaatccct taatgtcgac atctaccaca
     1681 tgatgacatt tgcatttatg tgcaataaaa ttacatcttg ctattgcatg ttctgtagag
     1741 cgattaggtc ggaacccata ggaatgtttg aaaaattttg cttcacatat gggttctaac
     1801 acttgtttaa acatttgttg tatcaagcgg tctttcatag tgggaatgcc tagaggacga
     1861 gttttcccat tactttttgg aatgttgact cgtctaactg gctgcggctg ataattttca
     1921 attgtttttc gaatatcatt tacaaacttt tgtttgtttt gaaatttgaa atcactgatt
     1981 gtttgattat ctatcccaga tgttttagaa ccagtatttg atttaataat tctatatgct
     2041 agtaggatat tttcctctga cataattatt ttataaaggt caataccttt tgttgcttgt
     2101 ttgctacttc tttcatagag ccaatcaaaa gtttcttgca tttgatagta ttcataatat
     2161 cgtaattctg tactcactga tggattattt ctcctaactt taaaattagt cccatcctct
     2221 taccagatct tgtgagtttc atttaactgt attcactttt caaagaacaa gtcttggggc
     2281 tatccctcca cgttgattag acgcttcaca ggtactgtgc ccctactctc gcagaaatta
     2341 aggattttcg actattagtc ttatatccaa cgctccaata acagttgttg aataagtgtt
     2401 ttctgttttc ctcgttccag ttattctagc catacatcta tccttaggtg cttactttga
     2461 gcctgaatat tatataaggt cattgttcct tatcccgaat ttcataaata cgactcttac
     2521 ttttcggtaa tattcgttgc ttttgcaacc caattatctt tcgataaaag tcatccttta
     2581 gacccgtaca ttcgcaagtt cgtcagttcc gtaggaacat tctcaccata gatagtttgt
     2641 ggcgtcccgg catatccttt accatataag ttattactta cttaggtatt gttctgccga
     2701 ctttacctag cttcacacga atattcactg ttatgaatat tcgcatgtag gagtatcaga
     2761 ccagttgctt caaccaactt gacggttttc atttcaactg tgaaataact agttgtaatt
     2821 atctttagtt cttgataatt ctcccatttc atgcctatgc acttataggg aaacgagtcg
     2881 caccatattg gatatagtca ttcatgtatc gcttgctagc tggcaaaaac tcttcatgaa
     2941 gtcccatcat atcgccaaag ggacgaacca attttacatt ggtatcatca taaaaatcta
     3001 aaacctcatc acaccgacgt tttaattcat ccaatgattc tgcactcact cgaacaacat
     3061 agcttaattt gtacatggat tctttggatt gatccaacgt tgtttctagt tcatccacgg
     3121 agtccaacgc atccaagaca ttggaattgg tttcattgtc ggactggtac gcatggttgt
     3181 ctaagtcttt cagttctttt ttcttattgc gaactgtcgc cagtgccttt ttattcgtca
     3241 cgatttctac attcatactc gtgtcgattg ggaaagtgaa ttgttgttgc tggtaataaa
     3301 agagttcaga agatggaaac tccatctcac cgacaatcgt attaatcgtt agataggcca
     3361 cataagattc atgattttca tgttccatgt gtaagtaccg aggcttctct tcaatcaaac
     3421 agcgacttgg gcgaagtagg tcatagcgct ttatcaatgt ttcagatttt agctttttct
     3481 tagacaactg aaattcatat tcttcaaatg gtgttccttt ttctccataa atgtgttcaa
     3541 tcaaataagt tagatcacta ggtgttactc gtcttacttt gaatctacga gccaatttac
     3601 tttccatcaa tttttcaagc ttagtatacc ggcgaatttc ttcattggat aacgaaacaa
     3661 aatcacccat caaatgatga ttcactccat agacaaactc ttgaagtccg gataaaaaag
     3721 agttcttgag acttttcagg tttacttctt cgtctgtgac aatcagtttg aagccaataa
     3781 aaaagcgata gtcaatttga ctatcgccaa tcatcgatac caatgcatcc gtctgtaagt
     3841 cgattctctg ttttgcgact tctttcaaac gaccagttat ctcttttttc gaccgttcct
     3901 gcgtggcacg aatgctgctt tctgtcgcaa tttgtaacgc atgaatctta ccttcacgat
     3961 tttgcgcaat caattggcga aagttatcgt ggacttgata cttctgttca agtgataaga
     4021 aggagtaatt gtaagggact agttcgtagt aagcaaagca ctctccctct tggttaaaaa
     4081 caagattatt ttcaatatat ttaatcgggt acataattca cactcctcac tagggtgatt
     4141 ggttcatccc atcgtttttt ctgttctttg actttctttc ctgcaaaagt aaccttaaat
     4201 cgcaagtgat aactaataaa ggatcgtaaa aaccctaatg gcttctttcc atcaaaggtc
     4261 ttttgcgaca taaaccaagt gactgccaca ggtatgccca agtattttaa aaatgccccc
     4321 tctatcatag atagtggagg catatgagca aacacaatga caaacagcaa ggataagaca
     4381 aaccaagcca tttgattaaa ggttacagga aaaggcagct gaaagtcatt gattgcgtaa
     4441 atgacttttt caactgccca gatactggta tagcttttta tttttttcat tagtttcact
     4501 tcctaaaaaa gcgcctgata aagacgactc tgcatgaata ttaattttct ttttaaaagt
     4561 gtattccaac tttcaatcta tcaactgagg tttatcagac cataatctgt ggatttcttg
     4621 gtatccagct gaagtaataa aagtatatct ttgttgatat attctatctt gaaaatcact
     4681 aaaccgtatt tttatttccc atttactcac tgaatattta gggatactca ttacttcgtt
     4741 attatatttc atccaatata attcaaattc tgtttttttc cctactctta gaatatctgt
     4801 atcaataggc gctgaaactc ctaacgcatc cagactatca tcaatatgtt tttgagccga
     4861 aagtaatgat aatagattta cttcatggtc gtttgtgttg atagtttctt ctaaatacac
     4921 aaagtcaatt gaaaaagcca ttcccagacc ttcattaata agctcaaatg gtaatttaaa
     4981 aacatcattt tcgtcagact ttaccatttt accagcaaaa cggtgttcag atattttggg
     5041 tataagtttt aaataagggt aataacttag acggtcctgc tcttcatttc tttgtttttc
     5101 atcagcatat tgttgctttg ataaatcaag tgattcctca tgttgtctct cttgctgttt
     5161 catcgctttt ttatgttgtt tatttgtaaa aaaaagtgtg ataaaacata ctgcaaatga
     5221 agttagtaca gggacaaata tgtctataaa tgatttaagg tcaaagtcaa aattcataat
     5281 aatcataacc tccagcatta tatatttatc aataattata tccctaaacc tatatcatga
     5341 aaatgtttat cctatgaatt caaatgttcc tctatttgtc acaagataat ttccttctag
     5401 ttccaagtca cgtccatatg cttcatagtc aatatagttt tgcaagtgtg taggcacctc
     5461 tccaaaagca cctgtttctt caatcaaata atacgccaca tcactcatat cgttacaatc
     5521 gggataacag ataatatcat ccacatgctc caccatttcc tcaaaagagt tgaagaaagc
     5581 atgttggatt tccaaagcaa cttcaccaat cggtgttcct tctagttcct ctgctaaacc
     5641 acacaaccga ttgatttcct caattggtgt atattcatca atctcaaaag gcaactcata
     5701 atcatggatc gcatactctt cgtattcgtc atttaaacca atccgttctt tcacttcgtc
     5761 aaaatcgaca ggtgggctaa accaagcccc caccaattca ccttcgttat attttccaag
     5821 gttcgcaata tatacacgca tctgctccat aatggtcccc cttacgcacc gacaatccga
     5881 ttaaacaagt tgagtaagac atctttcaca ccagaagcgt tgaagaccag tcctaccgca
     5941 atcagcgcaa tcaccaaaaa accaatcagc ttggaaaact cacgtttgaa gcctaaaaat
     6001 aaaccaatga ccacaattgc caataagact aaagattg
//
LOCUS       DAFEZB010000113         5639 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFEZB010000113
VERSION     DAFEZB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.115"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..184
                     /locus_tag="LP859_002644"
     CDS             <1..184
                     /locus_tag="LP859_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL3030528.1"
                     /translation="GVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQCPTCGKKYT
                     LSYGDYILIEEKGKKR"
     gene            184..435
                     /locus_tag="LP859_002645"
     CDS             184..435
                     /locus_tag="LP859_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030529.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            451..888
                     /locus_tag="LP859_002646"
     CDS             451..888
                     /locus_tag="LP859_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030530.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            1331..1510
                     /locus_tag="LP859_002647"
     CDS             1331..1510
                     /locus_tag="LP859_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030531.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1578..1967
                     /locus_tag="LP859_002648"
     CDS             1578..1967
                     /locus_tag="LP859_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030532.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEIAVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGITEEKVVWVNNHTGLGALAFEGTVLDNISIVFEEESF
                     TLECDNLIRYIDPEFYEDKNLAWELAL"
     gene            1949..2215
                     /locus_tag="LP859_002649"
     CDS             1949..2215
                     /locus_tag="LP859_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030533.1"
                     /translation="MGTSFIDRVIVQVKEERKITMKYNSLDHKRMWVEKLEEDLEQLE
                     LLGYNKTSTVYKEGLKRTKKVQKELATLESSVQKKEVELTHAYL"
     gene            2225..2884
                     /locus_tag="LP859_002650"
     CDS             2225..2884
                     /locus_tag="LP859_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HBL3030534.1"
                     /translation="MADKRKEKIQKLLALAADANDEESMSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLASIIAQNFRTEFYYREGSPIDLCFTGLA
                     SDVQIAEITFQYASGSVSYCARKYMQQPEIKRKRKRKWQLKQDYIQGYLSGLSKVFKQ
                     QVLTNGYELALQLPEAVKSYMEDLGLVPGKDTSHKVKDNEAYFHGYQEGLKFKKTELL
                     E"
     gene            3191..3808
                     /locus_tag="LP859_002651"
     CDS             3191..3808
                     /locus_tag="LP859_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3030535.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTPKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            4397..4597
                     /locus_tag="LP859_002652"
                     /pseudo
     CDS             4397..4597
                     /locus_tag="LP859_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5084..5263
                     /locus_tag="LP859_002653"
     CDS             5084..5263
                     /locus_tag="LP859_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030536.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(5309..>5639)
                     /locus_tag="LP859_002654"
     CDS             complement(5309..>5639)
                     /locus_tag="LP859_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3030537.1"
                     /translation="AQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGV
                     LLAKKAGRYKGRPIEYSENSTDPQKRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYK
                     IKKANDL"
BASE COUNT         2030 a          872 c         1134 g         1603 t
ORIGIN      
        1 gggtgttccc ttcagcgaat tattcccaaa ctatcatgca ccacatactg tcgaatgcga
       61 ttgtggagaa agagcgaaat gtatcaaaag ctattgtttg tatcaatgtc caacatgcgg
      121 gaaaaaatat accttgtctt atggcgatta tattttgatt gaggagaaag gaaagaaacg
      181 ctgatgaaaa cacaacacaa attattgact gtaaaagaag tcgttcaaat tttggtggac
      241 aaagaagtca tttctgaaaa agtagcagaa gctttactta ttccgcggta ctggacgata
      301 gaaacccctt tagtagagga tgcaccgact atgctatttg aagacctagt gaagattgca
      361 ggatgtgaac tgcattatca agaaaacttg gaaacttatg aaaacgaata ttggttaacc
      421 aaattagaga actaaaaagg agaaaaaaat atggctcaaa attactatga cgaatttgtc
      481 aaattacctt tggataaaat ggcacaaaaa atggaggata tgacgttcct atacaacgaa
      541 acacgtgttc caaaaaaaca ttataaagaa aaattgtcgg tagctgtgga agaaatgatt
      601 gaatcaggtg tcgaaatgaa tcttattgcg acgtactatc gaacactgga agaactgaaa
      661 aagcaaaatg caaaatggtt tttccaagcc ttactatgct ttgaaatagg tgtcaaacca
      721 agcactatta aaccttctga ataccaggct ttagaactaa cgtatgcgaa atttatcgaa
      781 acgaaaaaag ccaagactgt ttcaagtgaa tggttagatt attttgaaaa tatcaataag
      841 tatggcgcat attacaccat gaaaaaggaa gacaacgaaa atgaataagg tgatttatca
      901 actaacaacg gattccatat caacgatttg aaaggaaaaa aggtcaataa ccatttatac
      961 cgaaaagtga ggatgtgtga aataagaaca aaaaaccttg aaattaaggg aatggttctt
     1021 atttcgcacc aattgctcca accgcccccg ccgcttaggt ggtgcgaaat aagcgggcaa
     1081 agcccgataa cctaagagaa agaaggagat ccatatgaat aaaatcattc ctctattgtt
     1141 cgtactgttt gttggcgaga tacttatgcg tcatggtgca gctctacatt taaacttcgt
     1201 agtattctta ggaatactgg tattttgttt gatcttttga cgctttgtgt taacccttgt
     1261 gagtctaata ttcaggttga ttgcagccat atttttcata gggttatttc gatgaaagga
     1321 gagagtacca atgccgattg caataggaaa taaacggtta cctgttacgt tggatgaaaa
     1381 gagacaaaaa gaattgcaac aactaaagca gaagtacggc aaaagtgaat ccaggattat
     1441 gtgtattgcg ttagatttat tgattgccca agaaaaagca ggatttgagg taccagcact
     1501 caaaaagtga cgtcactttt ttatactaaa acctaaaagt gatatcactt ttaaacaatg
     1561 gaaaaggtgg aaataagatg aagaaaaaac aaactaaact atcgttagca gatattttaa
     1621 cacagttaac cgcaactgaa gatggtgttg tagaatttga acgatctgaa attgcggtag
     1681 tagatgatcg ttactttaaa atgccttact tctttgacca agcaaaggtc atttgccttt
     1741 gtggctacga tggggtacgt gattactttg gaattactga agaaaaagtc gtttgggtaa
     1801 acaatcatac aggattaggt gcgttagcct ttgaaggaac tgtattggac aatatctcta
     1861 ttgtattcga ggaagaaagc tttacgcttg aatgcgataa tttaatccga tatatcgatc
     1921 cagaattcta tgaggataaa aacctggcat gggaactagc tttatagata gagtgatagt
     1981 gcaagtaaag gaagaaagga agatcaccat gaaatacaat tcattagacc ataaaagaat
     2041 gtgggtagag aaattagagg aagacttaga acagttggaa ttgttaggtt ataataagac
     2101 ttcaacggtg tataaagaag ggctaaaacg aacgaaaaaa gtacaaaaag aactagcaac
     2161 actggaaagt agcgttcaga aaaaagaagt agaattaaca cacgcttacc tttaggagta
     2221 gaaaatggca gacaagagaa aagagaagat tcaaaaatta ctggcgcttg cggcggatgc
     2281 aaatgatgag gaatcaatga gtgcattagc caaagcccag caattgatga tggactatga
     2341 tcttactgaa gatgagattt tcaattatca tgagcagcaa cggatagaag cagtagaaac
     2401 gcacgtgatc tatcgtggac gtccgcaaaa atggctgtat cgtttagcgt cgattattgc
     2461 gcaaaatttt agaacagaat tttattatcg agaaggatca ccaatcgatt tatgttttac
     2521 tggcctagct tcagacgtac aaatcgctga aattactttt caatacgcaa gcggaagtgt
     2581 ctcctattgt gcaagaaagt atatgcaaca acctgagatc aaacgcaaga gaaaacgaaa
     2641 atggcaatta aagcaagact atattcaagg ctatctcagt ggtttatcta aagtatttaa
     2701 acagcaggta cttacaaacg gatatgaatt agcattacag cttcctgaag ccgtgaagtc
     2761 ttacatggaa gatctcggtt tagttcctgg aaaagacact tcacacaaag taaaagataa
     2821 tgaagcttac ttccatggat accaggaagg attaaaattt aaaaaaacag aattactaga
     2881 ataagaagtg cgcccaacag ggtataccct gttgggctct tggtgttcta ttgaatgtaa
     2941 atgagtgtca tttttatatt taattggcac agttttttct aattatttgt catattcata
     3001 gtcaactata gctacaaaag caactaattt tggagataga ttgagatatt taaaaatcta
     3061 tcatgaaaaa ttagttgctt ttgtcactta gcgctgaaaa tttatgccat ttatctagaa
     3121 aaatgacaac agagttgggc aacagaattt aaaaatgatc cggatttcag aattttaaag
     3181 gagttttatt atggcgaaaa ttgggtatgc tcgagtaagc agcttagatc aaaatttaga
     3241 ccgacaatta gaattattat cagagtgtga taaaattttt agtgataaag caagtgggaa
     3301 agataccaat cgagaaggat ttcaagaaat gatgaaattc atacgtgaag gtgatattgt
     3361 cgttgtgaca gaattaaaac gacttggacg aaataataaa gagctaacag aaacgatgaa
     3421 tttaattcag ctgaagaaag ctacactgga agttttgaat ttgccagcgt taacaggtat
     3481 cactgatgat aatttacgtc gattacttaa taacttaatt atcgaactat acaagtacca
     3541 ggccgaagaa gaacgtcggt atattcgcga aacgcaacaa caaggaattg cactcgctaa
     3601 agcaaaaggg aaatacaagg gaggtacgcc aaaatatcgt gagaatgatc cgcgacttca
     3661 attagcattt aatttgtttt tgactggatc tacagataaa gaagttgaag agctaacagg
     3721 gattaatcgt agaacgttta gaagatatcg aatgaaacat ggtattacta atgaagttag
     3781 ggagcaaaaa aataaatctt tcagttgagg ctaatataat tttgcaagaa agggattttt
     3841 atgaaagaaa ttagattttc aaatcaaaat atgcagaatt actataatga aattatgaat
     3901 atggaaacta gtgaattgaa tttaattata gccgaaaaaa agttgcattg gataggaata
     3961 aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta ggtcttccac
     4021 aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat tctatattaa
     4081 cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt aagcatgttg
     4141 aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa tatgagaaca
     4201 ccacgcatag tttttatttt gagatatcct agaatgaaac acattaagaa cgaaagcaaa
     4261 gcaaaagacg gcagaacttc tactaccttc taagtttttt catatttgtt tgttatgcta
     4321 tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag gcagcgctct
     4381 ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc agctagcatc
     4441 aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg ttgactagac
     4501 atataaggaa aagaaatact tactttggct gtgccagtta gatatagata cctttgaaac
     4561 atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg aagccttctc
     4621 tcggttcgct tgttttgcta tttattagtt ggctcagggc aattctaagc tcgtttgaaa
     4681 gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat gtacgtatta
     4741 ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt gcagataaat
     4801 agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca tccagggcaa
     4861 caaactgctc cactttcata tttaatagat gaataacctt ctgtataata gaaaggcata
     4921 cctttcggta tgacaactta tacaggatgt tattttattg gttcactatc gaaaaatttt
     4981 ggagctttat ttttccggta tttctcaacg aacgattagt tcaactgtgg gaagctccag
     5041 acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg aactaaccgc
     5101 tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa caacagcacg
     5161 aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa agaatatgac
     5221 attgaaactc cttcatagag aatatggggt gcatggaaca tagaaacgaa aatgcacggt
     5281 agaaaagcga atgaatgtag tataaactct acaaatcatt cgctttcttt attttatata
     5341 tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact ccttgattta
     5401 acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag ttctcagaat
     5461 attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact ccttgccgct
     5521 gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat gataaaaaca
     5581 tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa aactgtgca
//
LOCUS       DAFEZB010000114         5460 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFEZB010000114
VERSION     DAFEZB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.116"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..177
                     /locus_tag="LP859_002655"
     CDS             <1..177
                     /locus_tag="LP859_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030538.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="LP859_002656"
     CDS             190..690
                     /locus_tag="LP859_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3030539.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="LP859_002657"
     CDS             787..2634
                     /locus_tag="LP859_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL3030540.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="LP859_002658"
     CDS             2637..3533
                     /locus_tag="LP859_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3030541.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="LP859_002659"
     CDS             3583..3972
                     /locus_tag="LP859_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3030542.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5460
                     /locus_tag="LP859_002660"
     CDS             3959..>5460
                     /locus_tag="LP859_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3030543.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         1864 a          789 c         1159 g         1648 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
//
LOCUS       DAFEZB010000115         5412 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFEZB010000115
VERSION     DAFEZB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.117"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..384)
                     /locus_tag="LP859_002661"
     CDS             complement(<1..384)
                     /locus_tag="LP859_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL3030544.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSL"
     gene            616..1608
                     /locus_tag="LP859_002662"
     CDS             616..1608
                     /locus_tag="LP859_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3030545.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1668..3089
                     /locus_tag="LP859_002663"
     CDS             1668..3089
                     /locus_tag="LP859_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL3030546.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3082..4581
                     /locus_tag="LP859_002664"
     CDS             3082..4581
                     /locus_tag="LP859_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL3030547.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4620..>5412
                     /locus_tag="LP859_002665"
     CDS             4620..>5412
                     /locus_tag="LP859_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL3030548.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLS"
BASE COUNT         1783 a         1010 c         1073 g         1546 t
ORIGIN      
        1 caacgatcca ttgattactc cccatttacc agccaaactg actaaatcat aattattatc
       61 atccataaca atcgtttcta taaaatttag ttctttcctg atttcagaat aagtggagta
      121 atctgtatat gtcagcggat gcttgaatat gtccgagaaa tttttggtcg aaccatacgt
      181 agaaaacgaa tattcaatcg ttctaatttt ccattcaacg ttagttttaa cttgcctgag
      241 caaattcttt ttgtctgtta aataactcga ttcgatcgat tcataggtat tctggtaaat
      301 gaataaacta aatcctatga caagtacggc tcctaataaa aacatcggta aaaatatttt
      361 ataaaatact gtacttttct tcattttctt cctctcctcc ttttaaaata aaaggctttc
      421 atttttctgt aaaattgata ctcatctaat taaatgagca ttccccgctt tcaagaatag
      481 cataattaaa aataaaaatc caagtaattt ttaacgtgtg caatcttttc atatcaaaga
      541 tctattgact tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt
      601 aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta aagcagctgg cgtttctcat
      661 acgacagttt ctcgtgcttt gaataacagc agtttaataa aaaatgaaac aaaacaaaaa
      721 atacgacaac tagctaagga aatgaattat tcgccaaact atagtgcaaa aggtttagtg
      781 aatcaaaaaa aatatctaat cggactattt ttctcaagtt tgcataaagg aacttcatca
      841 aatttcctag cagattccat tgcaggcatc cattcagtat tagacacaaa ttacagttta
      901 tctgttgaaa gtatcgatgt tgttgattta acagaaatca acctgcaacg ctacgatgga
      961 ctgatcgtta tgagtcaaag tgatgatgat cagcctttca tcgactatct caaagaaaaa
     1021 gaatttcctt tcgttgttgt caatcgtcac atcaatgatc cagcggttat caacgtcact
     1081 gccgatgatg cggccggtgt tatgcaggca gtagattacg gtattggatg cggacatacg
     1141 aagatcgctt atattggcgg aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg
     1201 gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc cagcctattt tttaacagga
     1261 gattacagct tagaaagcgg cctccaaaac atggaatacc tattatcact ttctgacctg
     1321 cctactctgg ttttttgcgg caacgacgac atagcgattg gtgcattgag agctgtacat
     1381 ttagctggcc tccgtgttcc ccagaacatt tctctgatcg ggttcgatga ttctccagtc
     1441 gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa
     1501 gaaggaacaa agcttttact agatttgatc gaaggcagga cgatcgaaca atctaaaatt
     1561 gagctctcca ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat
     1621 tttatataaa aatgttaacg tgtgcataac acagaaagga agtttatatg aaaaaactga
     1681 caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca
     1741 attttatgcg tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta
     1801 aaggtaatgt tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc
     1861 aagataattt atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt
     1921 ccgagattat caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc
     1981 tatctctagc agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa
     2041 tccaattcgt ggagactgat aatttaacag atactccccc acagagcctc ccgggcaaac
     2101 tgacagcctt tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt
     2161 gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa
     2221 aatgggattt agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta
     2281 gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac
     2341 ggcataacta tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg
     2401 aagggcctaa agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca
     2461 tcacagatga tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata
     2521 cagcgatggt ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag
     2581 atcctttatt ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt
     2641 ctttacctaa agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc
     2701 cttttgccca tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc
     2761 gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca
     2821 ctgtttcact agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg
     2881 atgaaaagac gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc
     2941 gattattgca aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag
     3001 atacagttca agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag
     3061 aaatgaaagg aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga
     3121 cgagacagtg tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat
     3181 cagcaaacga tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat
     3241 ttgaaagctg gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac
     3301 gtaactgccg gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg
     3361 gattatcgat atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt
     3421 caaggttact tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct
     3481 accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat
     3541 ccagatctgg gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag
     3601 cttggaaaag atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat
     3661 gccggaggag ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa
     3721 aaaattttag gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac
     3781 gatgagatcg aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac
     3841 cagcgcatat cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat
     3901 ggtctatcgg gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct
     3961 caaggaggca gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg
     4021 atggcacgag cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc
     4081 aaacagtatt tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa
     4141 gcaggtggga tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc
     4201 tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta
     4261 ttgcaagctc ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag
     4321 ttggtcttgt ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag
     4381 gtatctacga atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc
     4441 gtcttattag aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct
     4501 gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt
     4561 aaaaacggag taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta
     4621 tgtttttatc tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct
     4681 acgcaaaaaa catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg
     4741 aaaacaacaa tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc
     4801 gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg
     4861 aaaaattttt agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt
     4921 ggtcacatct tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg
     4981 cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg
     5041 aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact
     5101 tgcactatca tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc
     5161 gtccagataa agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg
     5221 aagaagcagc tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc
     5281 ggatagaata cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac
     5341 gctataaaga agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca
     5401 aactattatc tt
//
LOCUS       DAFEZB010000116         5304 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFEZB010000116
VERSION     DAFEZB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.118"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(1273..1938)
                     /locus_tag="LP859_002666"
     CDS             complement(1273..1938)
                     /locus_tag="LP859_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030549.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="LP859_002667"
     CDS             2118..4100
                     /locus_tag="LP859_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL3030550.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="LP859_002668"
     CDS             4104..4742
                     /locus_tag="LP859_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030551.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="LP859_002669"
     CDS             4770..5198
                     /locus_tag="LP859_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030552.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1750 a          805 c          965 g         1784 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatc
//
LOCUS       DAFEZB010000117         5137 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFEZB010000117
VERSION     DAFEZB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.119"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1568
                     /locus_tag="LP859_002670"
     CDS             <1..1568
                     /locus_tag="LP859_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3030553.1"
                     /translation="FQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGI
                     PGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQAT
                     QEAFEAYLKEKGVQLGKQPGTILQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDT
                     LEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRY
                     VNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTIL
                     TQVQQLQLKAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKE
                     TKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIK
                     VEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQKF
                     VSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAACT
                     SEACDFKRSEGFI"
     gene            1684..1833
                     /locus_tag="LP859_002671"
     CDS             1684..1833
                     /locus_tag="LP859_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030554.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1914..2510
                     /locus_tag="LP859_002672"
     CDS             1914..2510
                     /locus_tag="LP859_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL3030555.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            2523..3422
                     /locus_tag="LP859_002673"
     CDS             2523..3422
                     /locus_tag="LP859_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL3030556.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            3425..3909
                     /gene="ssb"
                     /locus_tag="LP859_002674"
                     /pseudo
     CDS             3425..3909
                     /gene="ssb"
                     /locus_tag="LP859_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            3931..4188
                     /locus_tag="LP859_002675"
     CDS             3931..4188
                     /locus_tag="LP859_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030557.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(4347..4481)
                     /locus_tag="LP859_002676"
     CDS             complement(4347..4481)
                     /locus_tag="LP859_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL3030558.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            4624..4884
                     /locus_tag="LP859_002677"
     CDS             4624..4884
                     /locus_tag="LP859_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030559.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
BASE COUNT         1843 a          916 c         1066 g         1312 t
ORIGIN      
        1 gattccagca attacgagac gcagaagaga cctatcccaa atttgaagaa gcacaagcac
       61 gccagatcgc tgattggttg atcggcatga acggcagccc actgtatagt ttactcttgc
      121 agcaaaaagg cataccagga agtttttcct taggcagagt ccaaacccca acgctttaca
      181 tgatttatca gctgcaggaa aagattagaa acttccaaaa agaaccatat tttgaaggaa
      241 aagcccaagt aatcgctcaa aatggcgcat ttgatgcgaa gcttgatcca aatgaaaccc
      301 aagcgacaca agaagcattt gaagcctatt taaaagaaaa aggtgttcaa ttaggaaaac
      361 agccaggtac gatccttcaa gtcgaaacag agaaaaaaag tgcggctagc ccacgtcttt
      421 tttctttatc gagccttcag tctaaaatga atcaactcat gaaagctagt gccaaagata
      481 ctttggaagc catgcaaggg ttgtatgaag gaaaatatct aagctatccg cgaacggata
      541 ccccttatat tacggaaggg gaatatgcgt atctgttgga tcacttagac gaatacaagc
      601 attttttaaa agccgaagcg attccgactc ctattcatac accgaatagt cggtatgtga
      661 ataataagaa agtccaggaa cattacgcga tcattcctac aaaaacggtt atgacagcag
      721 cggcttttga acaactaagt ccactgcagc aggctattta tgaacaagtg ttaaaaacaa
      781 cggtggccat gtttgcggaa aagtatacct atgaagaaac aacgatcttg acgcaagttc
      841 agcagcttca gttaaaggca atcgaaaagg tgccgctaga tctaggttgg aagaagttat
      901 ttggaaaaga gagcgacgga aaagagaagg aagaagagcc gctacttcct aaagtgacaa
      961 aaggagaaat ggtgacggtg gatctacaag ttcttgaaaa agaaaccaag ccaccacaac
     1021 cttataccga aggtacgttg atcactgcaa tgaaaacagc tgggaaaacc gtggatagtg
     1081 aagaagcgca atccatattg aaagaagtgg aaggaatcgg tacagaagcg acacgggcga
     1141 atattattga aaccttgaaa caaaaggaat atatcaaggt agagaaaaat aagctcgtgg
     1201 tgacgaataa agggattttg ctttgccaag ccgtggaaaa agagccgctg cttacgagcg
     1261 ccgaaatgac cgccaaatgg gaaagctact tgttaaagat cggtgaacga aaaggaacac
     1321 aaaccacttt tctagccaat atccaaaaat ttgtctctca cttattggaa gtcgtaccag
     1381 ggcaaatcca atccactgat tttggatcga cgttacaaga agtgaaagca gccagtgaaa
     1441 agcaagaagc cgcgcgtcat ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac
     1501 tctatcaaaa ggtggcagct tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt
     1561 tcatttgaag caacgattgt tttaaaagaa gacttcacta caggctttga attttctgaa
     1621 aagaaaaaaa cgaacttcaa gaaaagaaca cgccgcactg caaaataatg gagggttaat
     1681 cttatgcgta ataccacaca caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt
     1741 tcagaacagg aaaaaatgcg tgccagcaaa cgaagactgc agcaattgaa gaaccagcag
     1801 aggattcaaa aagaaatcga attagatcga taaaatcttc ctaactattg tatacgtata
     1861 aacttatgtt atactgtttt tataagttta tacgtataca aaggagggag aaaatgacaa
     1921 aagcaatggt atacaagctg ctagaagaat ttaatggaac tttggctttg aaggatgcca
     1981 aaaaagcggg catttctcct gtaacgatca aacgtatggt agatcgaggg gagctggatc
     2041 gagagtatcc aggttttttc accttacctg gtcaatttcc tgatgaattg tttatggcgc
     2101 aaaaaaaata tgaacgcggc attatttcac atattaccgc gttagatttg tatgatttaa
     2161 cagatatgat ccctagacag atcgatctga ctgtacctta cggttatcac gtttctgaaa
     2221 aaggattgaa ggaattcgca gttgagctac actatacaaa agcggaatgg tatgaattag
     2281 gtaagattga aataaaaagt cgctatggga atcctattat agcttatgat ccagaaagaa
     2341 ctttatgtga catttggaat ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa
     2401 tcaaaaacta tatggagtcg gacagaaaaa atcttagaaa attgaatgag tatcgacgaa
     2461 ttttaccaac agacaaaacg atgcgttctt atattatggc tttaaattaa aggagaagat
     2521 acatgacacc tacgcagtta aaagcaaaag tgaagaatat ttctagagaa aaagaagttg
     2581 atccgcagct tgttttaaga cattttatga tggaaaaatt cttggaaaaa atctcagagt
     2641 ctccttatcg tgaaaatttt gtgttaaaag gcggcttctt gattggctca aagtatggca
     2701 ttgaaaatcg cacaaccaaa gatattgata cgactttacg ggaaatgaaa gtaaccaaag
     2761 aaacattaac aacggttcta aacgaaattt tttcaacgcc gacgaaagaa gacattcaat
     2821 ttgagattca aggaatgaaa gaaacgagag aagccgatta ctatccagga tttagcttac
     2881 gtgtgctggc acatttggaa aacatgcggc cagattttaa ggtagatgtg actacaggtg
     2941 actcgatcta tcctgcaacg atcactcaca gtcataaatt gatgtttgag gatcgaacga
     3001 ttcctttgga atcctatcct acagaacaaa ttattgctga aaaattaagt gcaacctttg
     3061 attttttaac agataattcg agaggaaaag atttttatga tctctatact attcctaaaa
     3121 tggagaagat cgatgagaag accttgaaag atagtgtgag aaatacattt acacgtcgag
     3181 gaaaaactga tccgttaaag atatactatg aaaaggatat ggatgtactg aaaaatagtc
     3241 acgagctaaa agatacctgg aagaaatatc aaaaaagcaa tccttatgct gcatcgatca
     3301 cgtacgatga gacaatggat tcgattagta atttgatgga aaaagtgatt cacgtcgaag
     3361 aaaaagagca aaaactagct tatcaaagac accaacagat gaatagagaa atggagagat
     3421 aaagatgatt aataatgtga cattagtagg acgcttaacc aaagatcctg atttacgtta
     3481 taccgcaagt ggtactgcag tagcgacttt taccctagcc gtcaaccgaa atttcacgaa
     3541 tcaaaacggc aatcgagaag ccgattttat taactgcgtg atttggcgca aaccagcaga
     3601 aacaatggcg acattagcta aaaaaggtag cttgattggt gtagttgggc ggattcaaac
     3661 gcggacttac gacaaccagc aaggacaacg tgtatatgtg actgaagttg tagcagataa
     3721 ctttcaacta ttagaaaata aagcagcgac agagagccgt gcgcacgctg atcaatcgtc
     3781 caccagtcca agcaccgcca cctttgaaca acggatacag cgacgcctaa caacaatgga
     3841 ttgaacgctt cacagaatcc atttggtgga caatcaatcg atattagcga tgatgatcta
     3901 ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa aaattagtct gacgatggat
     3961 ctattgatac ccgtaattgc atttatagac gggatttctt tgggcgtcac tgaaaatcct
     4021 ttggtaaata gaattgcgaa aggatcacta tttcttgtat tcgtgtatct tgtctgttct
     4081 tatcttttaa aaagagcagg aaaggataat tccttttggc gattgactaa agtcacagta
     4141 gcagcgacag ctgttatact tacggctatt ctaccgcttt atatgtagag ttaacgggaa
     4201 atcgttgaac ttcttgcgaa atattgctat aatgaacatg aaaaagagga gttgcgctaa
     4261 caacccctca ttaacagcac cgattaaaga acggtggcag aatattagtt atttggaaat
     4321 aaccgctgac ctgctaaagt agacggttat ttctttttgt catttttaag catatttaca
     4381 atcaaactaa tcaaagcaat ggtaaacata ccaaaaccaa gaatggtttg aattgtctca
     4441 tatgcggaca aaaggcttac tccttttata gattttagca cttacattca taagcaccac
     4501 cacctttcca gaagtcgcca ccgtcttcaa actttactgt cttgactatt atagcatatc
     4561 taaaatcgaa taaaaattct caattttaac tgtttttgct ataattttag cgaggtgatc
     4621 gtgatgaaca aatatgttgg tttgcttgat aaaatccgag taataaaaac acaaccatta
     4681 ttggttcgct ttactttaca aacaattcat gaatcgatta attgtgtagt tgcagatatt
     4741 gaaattatcg ataaattgtt aattatggat gatggaaaat acaatattgc cgtcacaggt
     4801 catttcaata aacggaatca attagtcatt gaatcgatgc aaattagaaa ccctgatcat
     4861 tttacgcgat caatgggcat ataaataaaa gaagagctgc aatcctaatg taggactgca
     4921 gctctttttg ttttacatag aaacaggaag aaagggaaag gcctgtttag gaaggcacaa
     4981 gtgccttctc aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa
     5041 aggaagtgaa cgttgcacgt tcactaagat aaggggtctt gctttgtaag acaaaatagg
     5101 aaaagcttcc tctcaccaaa aggttcgttc ctgttcg
//
LOCUS       DAFEZB010000118         5121 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFEZB010000118
VERSION     DAFEZB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.120"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            151..912
                     /locus_tag="LP859_002678"
     CDS             151..912
                     /locus_tag="LP859_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3030560.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQFLLTNTDENNIRTEYYHAWIEMKK"
     gene            1879..2147
                     /locus_tag="LP859_002679"
                     /pseudo
     CDS             1879..2147
                     /locus_tag="LP859_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            2819..>5121
                     /locus_tag="LP859_002680"
     CDS             2819..>5121
                     /locus_tag="LP859_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030561.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         1799 a          734 c         1088 g         1500 t
ORIGIN      
        1 aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca tattattcct
       61 ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt caatagaaaa
      121 aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat tggccaagta
      181 tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca aggattaatc
      241 cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga tatttttatg
      301 ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat gaagaattta
      361 tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag aagaatccat
      421 aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa aaaacagttg
      481 aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc ttttgactgt
      541 atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc taactattca
      601 agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg attttttatc
      661 gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa agatacggct
      721 gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa taatatttta
      781 gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat cggccagttt
      841 ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc atggatagaa
      901 atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt tttagttata
      961 aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt cgtggataga
     1021 tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg caattcttag
     1081 cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg tattgggtgt
     1141 tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt gttatgagtg
     1201 agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta cacttaaaat
     1261 acattgttgc ttacattatt acaatatgtt aataaggcaa aaataaatag atggattgaa
     1321 taaacatatt ttttaatcaa atagtcccta ctgtgacttt cctcagctat cggtgatact
     1381 accgatagca tttttttgct tataaaataa ttataagtat ttagagattt ttcttctaac
     1441 attcttcaaa caagaaaaat cacaaaggtt attgttattt ttatatattc tttttaagaa
     1501 tgattgtttt tttatagaaa ttttaaaaaa taaaacacgt tattgatgga tagttagttt
     1561 gttatttggt aatattatag aagttgactg gtagtgattt atttttcgtc gttttataaa
     1621 aatgttattt tttggaaaaa ttcacaaata tgtaacgaat gtttttctgg aatttgctgt
     1681 gttaaaaagc agtatgacta atataaatac tattgtcatt tatgcaattt tttatggata
     1741 gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt gcaaatacgc
     1801 cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat catcttggta
     1861 gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac caatggaaaa
     1921 atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta tgaacttagt
     1981 gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca gaaaatgatg
     2041 aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac cgagccaata
     2101 aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa agtgaaacta
     2161 cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa tgaaccatcg
     2221 attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga gaatgaagga
     2281 acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat aagcgatcag
     2341 ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa gcattgatat
     2401 atttttttgg gattgatgaa ggttaaccaa tacagggact cactgatgaa attctagagt
     2461 ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa aagtatataa
     2521 gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca ctggcaggag
     2581 gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca tacaaagcta
     2641 tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc ttacattgca
     2701 acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag ttggggaatc
     2761 tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg gagtaatagt
     2821 gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg tgatcaatca
     2881 atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc gttcgctgac
     2941 gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc gagtcagagg
     3001 aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga ttgaagggga
     3061 agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc attcaggtaa
     3121 atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa tagaaatcag
     3181 tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca caagtgaatc
     3241 ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat catctgagcc
     3301 atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt atgacatcgg
     3361 catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa agggatacat
     3421 tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac gagttcctag
     3481 agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt cacctttaaa
     3541 gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct ttgattatga
     3601 aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag ttgctttctt
     3661 tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtcttactt atgctgagcg
     3721 agcagcaaac acggaatttt caattacgcc tattccttca tcaagaccca ttttttcgaa
     3781 attatactat ggctctagag aaatcgacag ccaaggaaat cttgggaatc tgctgattga
     3841 cgggcaagac gtgaccaaca gtaataaatt tattttgcag gtaaattatg aaggatcacg
     3901 aaaagacggt gtagtggtta ccgatgtact accgcaagga acaactttgg caccgaatcc
     3961 gactacatgg agttccactg gaaatagaga acctatttca ggtggggttc ggatcgcaga
     4021 agtaacgaat ccaggtgaag caaacgttag aactcattat gtttccgagg aatttgtgga
     4081 caaagtatct tttaataagg aaacgaatac acttgttgtg gactttggta atctagcaga
     4141 gggtcaatcg tattttattg aatatgctct tgaagtgacc gatgtcgact tgttcaatga
     4201 aggaagacaa gaggatgggt tttctcggaa tcgagcgagt atgacctaca ataatcaggt
     4261 aatgagccga gactaccgct tgagaatccc tgaaagtaat cttccggcaa tctctttaag
     4321 aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat gaactaacgt atactctgca
     4381 tgtctcgatg ttttctggaa caagaaatac gcttgttatt gaagatgttt tggaagaagg
     4441 catggaattt gtgtcatttc tatcagatat tccctctttc attgatgccg aagtcaataa
     4501 tggaatagtt cgtttcagtg cgaataatac gattggtggt ccgacaagtt ttaccctctc
     4561 ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa attttaaact ttgcaacgct
     4621 tgtgaatagt gacaatcgat tgaatacgag tactgtaaga acaaggaaaa tcgatgggcg
     4681 tattaaagta attaaaactg atgagaatgg ccaaaggctg gcaggagcaa aatttgaaat
     4741 tttatcaagc gattcacaag tgatacaaga aggtataacg gcagaaaacg gagaattttt
     4801 aagtgaaccg cttcctgtag gtagctatca agtccgagaa acagaagcac cggaagggta
     4861 tcttttagat gcgacggtac atgatgtttt tataaatgaa tactccatag aagaagtgac
     4921 gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa ctaattaaac atgctgaagg
     4981 cagtggtgaa gtccttcaag gagctgtttt tgagttacag aaccgagaag gagaaacgct
     5041 acaaaccggt ctaactacag gagaagatgg aaaactggca attgacggtt tagcaccagg
     5101 agcatatcag ctggtagaaa c
//
LOCUS       DAFEZB010000119         5025 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFEZB010000119
VERSION     DAFEZB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.121"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..165)
                     /locus_tag="LP859_002681"
     CDS             complement(<1..165)
                     /locus_tag="LP859_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030562.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /locus_tag="LP859_002682"
     CDS             complement(367..762)
                     /locus_tag="LP859_002682"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL3030563.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /locus_tag="LP859_002683"
     CDS             complement(772..1620)
                     /locus_tag="LP859_002683"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL3030564.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="LP859_002684"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="LP859_002684"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBL3030565.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="LP859_002685"
     CDS             complement(2474..3334)
                     /locus_tag="LP859_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBL3030566.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..4741
                     /locus_tag="LP859_002686"
     CDS             3572..4741
                     /locus_tag="LP859_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3030567.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1616 a          940 c          634 g         1835 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat tgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     4081 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     4141 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     4201 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4261 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4321 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4381 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4441 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4501 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4561 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4621 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4681 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4741 actgtaatta acagaatgga ttttccattt acatataatt cttgacgcta ccataatcta
     4801 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4861 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4921 ctagcaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4981 cactttcaat aagaaaaaag tctttacttc catggataat aataa
//
LOCUS       DAFEZB010000120         4952 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFEZB010000120
VERSION     DAFEZB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.122"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(207..1169)
                     /locus_tag="LP859_002687"
     CDS             complement(207..1169)
                     /locus_tag="LP859_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3030568.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LP859_002688"
     CDS             complement(1171..2610)
                     /locus_tag="LP859_002688"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL3030569.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LP859_002689"
     CDS             2795..4741
                     /locus_tag="LP859_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL3030570.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1604 a          830 c          804 g         1714 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactcgat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat ga
//
LOCUS       DAFEZB010000121         4860 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFEZB010000121
VERSION     DAFEZB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.123"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1763
                     /locus_tag="LP859_002690"
     CDS             <1..1763
                     /locus_tag="LP859_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBL3030571.1"
                     /translation="FQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGI
                     PGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQAT
                     QEAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDT
                     LEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRY
                     VNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTIL
                     TQVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKE
                     TKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIK
                     VEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKF
                     VSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACT
                     SEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTG
                     FEFSEKKKTNYKKRTRRTTK"
     gene            1779..1928
                     /locus_tag="LP859_002691"
     CDS             1779..1928
                     /locus_tag="LP859_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030572.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            2004..2597
                     /locus_tag="LP859_002692"
     CDS             2004..2597
                     /locus_tag="LP859_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL3030573.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            2610..3509
                     /locus_tag="LP859_002693"
     CDS             2610..3509
                     /locus_tag="LP859_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL3030574.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            3512..3985
                     /gene="ssb"
                     /locus_tag="LP859_002694"
     CDS             3512..3985
                     /gene="ssb"
                     /locus_tag="LP859_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3030575.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            4351..4611
                     /locus_tag="LP859_002695"
     CDS             4351..4611
                     /locus_tag="LP859_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030576.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
BASE COUNT         1814 a          851 c          989 g         1206 t
ORIGIN      
        1 gattccagca gttacgagat gcagaagaga cctacccaaa atttgaagaa gcacaagcac
       61 gccagatcgc cgattggttg atcggcatga acggtagccc gctgtatagt ttactcttgc
      121 agcaaaaagg cataccagga agtttttcct taggcagagt ccaaacccca acgctttaca
      181 tgatttatca attacaggaa aagattagaa acttccaaaa agaaccatat tttgaaggaa
      241 aagcccaagt aatcgctcaa aatggcgcat ttgatgcgaa gcttgatccg aatgaaaccc
      301 aagcgacgca agaagcattt gaagattatt taaaagaaaa gggggttcaa ttgggaaaac
      361 agccaggtac gatccaccaa gttgaaaccg agaaaaaaag tgcggcaagt ccacgtcttt
      421 tttctctatc cagccttcaa tctaaaatga atcagctcat gaaagctagt gccaaagata
      481 ctttggaagc catgcaaggg ttgtatgaag ggaaatatct aagctaccca cgaacagata
      541 ccccttatat tacagaaggg gagtatgcgt atctgttgga tcacttagac gaatacaaac
      601 actttttaaa ggccgaagcc attcctactc ctattcatac accgaatagt cgatatgtga
      661 ataataagaa agtccaggaa cattacgcga tcattcctac aaagacggtc atgacggcag
      721 cggcttttga acaattaagt ccactgcagc aggctattta tgaacaagtg ttaaaaacaa
      781 cggtggccat gtttgcggaa aagtatacct atgaagaaac aacgatcttg acgcaagttc
      841 agcagcttca gctaaagaca gtcggaaaag taccagtaga tctaggttgg aaaaagctgt
      901 ttggcaaaga gagcgacaga aaagaaaagg aagaagaacc gctacttcct aaagtgacaa
      961 aaggagaagc ggtgacggta gatctacaag tccttgaaaa agaaacgaag ccaccacaac
     1021 cttataccga aggtacgttg atcacagcga tgaaaacagc tggaaaaacc gtggatagcg
     1081 aagaagcaca atccatattg aaagaagtag aaggaatcgg tacagaagca acacgagcaa
     1141 atattattga aactttgaaa caaaaggaat atatcaaggt agaaaaaaat aagcttgtgg
     1201 tgacaaataa agggatttta ctttgccaag ccgtggaaaa agagccgctg ctaacaagtg
     1261 ccgaaatgac cgccaaatgg gaaagttact tattaaagat tggtgagcag aaaggaacac
     1321 aagctacttt cttagctaat atccaaaaat ttgtctctca cttgttggaa gtcgtaccgg
     1381 gacaaatcca gtcaacagat tttggatcaa cgttacaaga ggtgaaagca gccagtgaaa
     1441 aacaggaagc aacgcgtcat ttaggtgttt gtccaaaatg tcgggaacaa gaagtcttgc
     1501 tttatcaaaa tgtggcggca tgtacgtctg aagcctgtga ttttaaatta tggacaacga
     1561 ttgccaaaaa gaaactcaca gccacacaac tgaaagagat cattcaaaac ggccgaacaa
     1621 gccaatcagt cagaggacta aaaggtcaga aaggttcgtt tgaagcaacg atcgttttaa
     1681 aagaagactt tacaacaggc tttgaatttt ctgaaaagaa aaaaacgaac tataagaaaa
     1741 gaacacgccg cactacaaaa taatgaaggg gttatcctat gcgaaataca acacacaaac
     1801 aacaagaaat cgaaaatcaa aaagcaaaaa ttcgtttaga acaagagaaa ttacgtgcaa
     1861 ataaaagaag actgcaacaa ttaaagaacc agcagcgcat tcaaaaagaa atcgaattag
     1921 atcgatagac gttaatcttg ttacaaataa actactatga tatatttgat gtagtgtatt
     1981 tgtaacgaag ggaggggaag taaatgcaag ataaactgaa agaattgttg gctattcgcg
     2041 atggaaattt atctatgaag gaagccagga aatttggaat cgcagcaacc actgtacaaa
     2101 gattggtgaa tcaaggaaaa ttgatcaaag ttgatcgtgg ttattatgta gaggatggtc
     2161 atggaataga tgatttattt tggcttcagc aacgattttc caggggaatc ttctctcatg
     2221 aaacgacttt agatatttac cagctttcta cgaatatgcc gaaattcatt cacttaactt
     2281 ttccccatgg ctataacgtt gatcgttcaa ttacaaaaga gcagcagtta caatttcatt
     2341 tcgtgaagaa agaagtttat gaactaggaa aagtagaggg aacttccttt caaggaaatc
     2401 ctattactat gtatgataaa gagcgtacac tttgtgatat ttggaatccg agatatgaat
     2461 ttgaatatga aatgaaatta gaagcattaa aagagtacat ggaagaccca ttgagagatc
     2521 caagtaaatt aagagattac atgacaaaac tacatgtaga aaaagaaatg aaataccaca
     2581 tgcaaagctt atattgatgg aggaaaaaga tgacaccgac acaatttgca gcaaaaacaa
     2641 gaaatttagc aaagagtaca ggattaaatg cacagttagt gcaacggcat tttatgatgg
     2701 atcgtctaat tgaacaaatt tctgaatcaa agtacaaaaa tgacttcatt ttgaaaggcg
     2761 gctttttatt aggatcaaaa tatggaatcg atagaaggac aacaattgat atcgatacga
     2821 catttagaaa tagcaaatta actgaagaaa aattgaagag catttttaat gatttaacta
     2881 gaaaacccac aaaagaaggc attgttttct ctattcaagg aatgaaggaa acaagagaag
     2941 ccgattttta tcctggattt caaattaaat tggtggctaa tttagagaaa gtacgtgtac
     3001 cgtttaaaat ggacgtgaca acaggagatt ctatttatcc tgaagtagat acacactatc
     3061 accaattaat gtttgaagat aaaaaagtgg agattccagc ttatccaaca gaacaaatta
     3121 ttgcagaaaa gctaagtgct acgttcagct ttggcacaga taattcgaga gcaaaagatt
     3181 actacgactt atttaccatt ccgaaactag aaaatatcga tacgaaagag ttatacaaaa
     3241 gtgttcgaaa tacatttaca aaacgtgggg atacaaaacc gttgtccttt tattatgaaa
     3301 gagaaatgga taagatcaga ggaaatgact atttggaaaa gcagtgggaa aaatatcgct
     3361 cagttaacac gtttgcgaat tccatttcat ttacagatac agtcaacgaa attgatcacc
     3421 taatgaaaag tgtgatcaaa attgaaaatc aagaaagaaa acggcagttt aaaagatcac
     3481 aacaaatgaa tcaagaaatg gagagataaa gatgattaat aatgtgacat tagtaggacg
     3541 cttaaccaaa gatcccgatt tacgttatac tgcaagtggc acagcagtag cgacttttac
     3601 cctagccgtc aaccgaaatt tcgcgaatca aaacggcaat cgagaagccg attttattaa
     3661 ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa aaggcggctt
     3721 gatcggtgtt gtcggacgga ttcaaacacg aacttatgac aaccagcaag gacaacgtgt
     3781 gtatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag cagcgacaga
     3841 gagccgtgcg cacgctgatc aatcaaccac cagtccaagc cccactacct ttgaacaacg
     3901 ggatacaaac aatggattgg acgcttcaca aaatccattt ggtggacaat caatcgatat
     3961 tagcgatgat gatctaccat tctaagtagc attttctatt ggttgcattt gctacggttt
     4021 atgttataat ttagtcaaca aaaagaattt tcaatctata accaaaaacc actagctggc
     4081 gggctagtgg ttttctttta cgtttctatt ataagaatta acgggtaggc ttactgtctt
     4141 tcttggcaaa tttacaatca agccatttgc agatatagtg cgcggctact tctgccatga
     4201 ttgcaaaaac aaaatcaaaa agaattttca aacctataac ctcctcccct tgccagatta
     4261 ggccggggta gcagacagca agactattat agcataattg aaatattcaa atgattggat
     4321 ttttgctata attttagcga ggtgattgtg atgaacaaat atgttggttt gcttgataaa
     4381 atccgagtaa taaaaacgca accgttattg gttcgcttta ctttacaaac gattcatgaa
     4441 tcgattaatt gtgtagttgc agatattgaa attatcgata aattgctaat tatggatgat
     4501 ggaaaataca atattgccgt cacaggtcac ttcaataaac gaaaccagct agttattgct
     4561 agcatgtatg ttagaaatcc cgatcagttt acgcgattga tgggcatata aaaaaagaag
     4621 agttgcaatc ctaatgtagg actgcagctc tttttgtttt acatagaaac aagaaaaatg
     4681 aaaaggcctt tttaggaagg cgcgagcgcc ttctcaattg aaaaacctct ctcactccaa
     4741 ccgccaccgc cgttcgagaa gaaaggaagt gaacgttgca cgttcactaa gataaagggt
     4801 cttgcaaagc aagacaaaat agaaaaaact tcctctcacc aaaaggttcg ttcctgttcg
//
LOCUS       DAFEZB010000122         4807 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFEZB010000122
VERSION     DAFEZB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.124"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LP859_002696"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LP859_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL3030577.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="LP859_002697"
     CDS             complement(3250..3768)
                     /locus_tag="LP859_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL3030578.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          785 c          730 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taatgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DAFEZB010000123         4805 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFEZB010000123
VERSION     DAFEZB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.125"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..411
                     /locus_tag="LP859_002698"
     CDS             <1..411
                     /locus_tag="LP859_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030579.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="LP859_002699"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="LP859_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL3030580.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="LP859_002700"
     CDS             2446..3708
                     /locus_tag="LP859_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL3030581.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4805
                     /locus_tag="LP859_002701"
     CDS             3953..>4805
                     /locus_tag="LP859_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL3030582.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1658 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaa
//
LOCUS       DAFEZB010000124         4616 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFEZB010000124
VERSION     DAFEZB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.127"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(15..962)
                     /locus_tag="LP859_002702"
     CDS             complement(15..962)
                     /locus_tag="LP859_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL3030583.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="LP859_002703"
     CDS             complement(1140..1943)
                     /locus_tag="LP859_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL3030584.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="LP859_002704"
     CDS             2089..3003
                     /locus_tag="LP859_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL3030585.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="LP859_002705"
     CDS             3051..3521
                     /locus_tag="LP859_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL3030586.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="LP859_002706"
     CDS             3606..4214
                     /locus_tag="LP859_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL3030587.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="LP859_002707"
     CDS             4348..4548
                     /locus_tag="LP859_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3030588.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttag
//
LOCUS       DAFEZB010000125         4560 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFEZB010000125
VERSION     DAFEZB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.128"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(132..464)
                     /locus_tag="LP859_002708"
     CDS             complement(132..464)
                     /locus_tag="LP859_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL3030589.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(1301..2386)
                     /locus_tag="LP859_002709"
     CDS             complement(1301..2386)
                     /locus_tag="LP859_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3030590.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIVKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(2706..3113)
                     /locus_tag="LP859_002710"
     CDS             complement(2706..3113)
                     /locus_tag="LP859_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030591.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(3132..3950)
                     /locus_tag="LP859_002711"
     CDS             complement(3132..3950)
                     /locus_tag="LP859_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3030592.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(4176..4469)
                     /locus_tag="LP859_002712"
     CDS             complement(4176..4469)
                     /locus_tag="LP859_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL3030593.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT         1395 a          743 c          650 g         1772 t
ORIGIN      
        1 ccccgaaggg aattgttgat agatcaagtc tactccttat ttttctagtg attctaagtt
       61 cctttagaag tctcgacaat attttcaagc aagcgaaaaa cgataaaata caagttatga
      121 gaatgatcaa ttcagctgat taaacgcccc atcccttttg cggtacaacg caagactcca
      181 ttttttcttc gaccaatcag tacaccgttt tggtcataaa catgtttgat tgttggatat
      241 agctctttta aagggatagg atcttttaaa attatttgtt tttgtttccc aatgtaataa
      301 atcatttctt ctgagacttt gtctttctgg attatatatt tcttacactg cacaacggtt
      361 ttactttgac cagttgatag tttcttcgtg atcttttctg tagcggtaca ccgtttaccg
      421 tagtgacaaa tatcttcgag actcaaaatt cctgtatatg acataaaata atccacccac
      481 ttactttaaa tatttttaac aagtttttct tgttattttt caaaaataga gaattgattt
      541 tcgttcatga caaacaaaaa agagggaacg cattcaaaca tgcgttccct ctaatttcag
      601 cgttcgtcat acggcaaaga gccccacgga catttttata acattatgct taggcataga
      661 ttcatcgtaa tatcacgtat ttttaacctt aacacagcca aaaaaagtgg tatcactttt
      721 gaatatctgt tatactaaat ccgttacggg taataccgta acactgaaag gtaaacgcat
      781 taaggggtat agacttaatg ccacctgtgg acgtcgacga aggttggtag ccttccgagt
      841 ctcacagggc ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc
      901 agaacgaaaa tcaattcatc acaagcatag cagacaatta aatttaaaac aactaaattt
      961 atacaaaaaa gtgtaattga atattagcag cactgcctga aaagaagtaa tatcaggctt
     1021 ttcagcttgg gtaaataagt gaattacctc tcttcatgaa ttattataac tcaaaaaaat
     1081 acatatgcaa acactagttc cctgtttttt tgaatgaaaa ataactaaaa tatctaacga
     1141 ttatgacatt aaaacctacg tttgtgattg ttttcataaa cttttaatct aaaaactccg
     1201 gaataataac tatttatttg taacatttgt aataaaatta aaattgtaaa aaagttctaa
     1261 tttaaaattg atcattttta tagtgttcgc tttttatcaa ctactctagc caattttccg
     1321 tactaactgt tggtaattcg ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa
     1381 tagctttttc aataaaccag ttttttacat acacaaaaat caaattctgc aaattattta
     1441 ctttctcagt cttttgtttt tgataagctt tcagcaacgt catatataat cctttatccg
     1501 catcaattcc gtaagattca agttcttcat acacaactac cgtcttctca agttcctcgg
     1561 ctttatgttt tgctttgtgt attgtggtga cggtttgttg ggcttcttcg taagtgttag
     1621 aaaatgtttt tatcaagctt aatacctttt ctggtacaaa tgtgtttatg ctgtcttctt
     1681 tcatgatttc aacgaagtta tctagcaaaa cttgattttg taattggtct gtttcagtgt
     1741 cttttaagtg tcttttagtg tctaaagatt tattaatata ataatcttgt gcgatttccg
     1801 catttccagt gttatcaagg gtttcagctg ttttttctcg acgtgcgatt tccgcatttc
     1861 cagtgttatc aagggtttta gcagtttcct ctcgacgtgc gatttccgca ttccgagaag
     1921 aggttgattt aacaatagtt tcgcttaaat cgtcatcgaa agaattttga ataaaaagat
     1981 catcggcact tatttcaggt tgaagtaaat agtatttatt tttttcattt tttttcgttt
     2041 ttgggttaaa ttctcctttt acgactgaaa tcaaattata tgtttctaac tcgtttaaaa
     2101 tggtttttac tttgccttgg tagcaattta atttgtccat taggtctgct tgtgtaaatt
     2161 ccaaatataa tagaccatca tcgtcgatcc atttttcatg taaggctcta cgaaattcat
     2221 ttttaaaaag catataggct atttttgagc tgtctttcat tttagagtat ttagaattct
     2281 gaattaaaaa actaggaaat ttataatatt tttcaatata aacattcgtt gctttatatc
     2341 cccaattttt aattatcatt ttttgagatt gattttctgt agacattaaa tttctccttc
     2401 ttcttagaaa cttgtatata ttcaggattt tcaacaagtg aactatacca tgtattaagg
     2461 gtgaataaaa cactttcagt atttaaggta taagttatat attaaaaaaa taagtataag
     2521 ttatacttta aatacaaata atgtataact tatactttaa ataataattt tataatgttg
     2581 atttaatcgc aaatttacac gttgtttatt aagttatacc ttaaatattt aatgtataac
     2641 ttatacttta atattcattt atctagtata acttatactt taattgaata ccgcatgaaa
     2701 aaaaattatt ttggtaattt ttcaaactct tcttgtattt cttctcttat gtgttcttct
     2761 tttgctactc ctagtctatg ctggatatat gagtctgcta atgtattaac aattttttcg
     2821 aaagaaattc tccctttttc actttgtaac tccgtcatat agtcttgtaa aattgagagt
     2881 ttgctattta atgccggaga gatcttttta ggaactgata ccttgtttct tttatcagga
     2941 attgaaactg gtcggcctct ttttttcaat gtaatatttc ttgtgttact caatgcacta
     3001 ttgctaaagt tatattcacc gcttattttc acatcttggc gtttgttatt gggtaataat
     3061 ggctcaaatt caccattctc catcatttta ggaatatcaa aatctttagg cattacttat
     3121 gcaactcctt attatttatt taaaaactct tgtctagcat ctagttctgc caaaatattt
     3181 ataaatactt ggtgtgcttt ataatcccat tgatcaaaat ttccattttt gtattttctt
     3241 aaatgtatac caattttaga ataccttctc aatctttttt gataatgaat gagatttttg
     3301 gataccacgg ttccgtattt ttcaattgct tcttctagga tttcttcatc aaaatcatca
     3361 tttggttcta ccatacaagg tacaatatct ataatttgca aatcaagatc gtatctatcg
     3421 accatgaatt tttgataatt tacatatttg ttgactccct ctaaggattc ttcagctgtt
     3481 tgaaatgaaa ttatcgaata gtctgaagct gccatagcat tatcggaaaa ttcgctaatg
     3541 gttggtggta catcaataaa tatttcttga taattgttct ttattggaag aagtaatttt
     3601 ttcaagacag taacttggtc taattcattt tcatgtttat tagcaaaagc ggagaatgat
     3661 cgaaaactag tgtcacatgc cagaaggtca agattttcat ttaccttaat aatactactt
     3721 gctaattctc catttgcgac accatttgtt atagtggttt tgatatcggt tataccgaaa
     3781 gttcttgcta tgacttgtgt taagttcgct tgagggtcta aatcaataat aagcgttttt
     3841 ctatttctta tcattgcatt gtcataagca agcatgctga ctatcttagt tttacctact
     3901 cctcctttaa aattgccaac tgtaaatgta gtcccttgaa aagtatccat gtaaatcctc
     3961 ccaatcttta attgataatt aaagtataac ttatatatcg gaaatggtca acattaaaag
     4021 tataacttaa ataataatat ctaaaaacgt ttggatatag cggtttttaa taataaaagt
     4081 ataagttata ccatatatat ttatggtata acttatactt ttattattaa aggtataact
     4141 tataccttaa atattgcttt tcgaaaatag ctattctagt ctatctgttt ctttactttt
     4201 cgctgctgat ttgaattgaa ttgatcatat tcattttcta gtattaataa cttttcgtat
     4261 acatcggtca tattcatact agaagcaaga cttagatcat atacaaatga agcaggtaat
     4321 gattttattc tacgttgtcg ggttatccag cttgcaaccg ttccttgtgg gtaggcatat
     4381 aactcacaaa attgttctac agtgattcct aaattcttga taatgtaaac attgatcggg
     4441 tgcgagtaaa caaatgtatt gatagacatg gtgcgtaagc ctactttctt ttattattgc
     4501 gtttgcaata atagtttaac acacgcatta ttctagtatc tactcctatt tgtaaaaaca
//
LOCUS       DAFEZB010000126         4485 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFEZB010000126
VERSION     DAFEZB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.129"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..244
                     /locus_tag="LP859_002713"
     CDS             <1..244
                     /locus_tag="LP859_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030594.1"
                     /translation="DDDHQHDDYGSYSFTLGDVQTYAQTSSVKEEQNFVVFVDYTIGN
                     PQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            255..1061
                     /locus_tag="LP859_002714"
     CDS             255..1061
                     /locus_tag="LP859_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030595.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            1084..1313
                     /locus_tag="LP859_002715"
                     /pseudo
     CDS             1084..1313
                     /locus_tag="LP859_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1334..2407
                     /locus_tag="LP859_002716"
     CDS             1334..2407
                     /locus_tag="LP859_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBL3030596.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            2451..2720
                     /locus_tag="LP859_002717"
     CDS             2451..2720
                     /locus_tag="LP859_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030597.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            2996..3271
                     /locus_tag="LP859_002718"
     CDS             2996..3271
                     /locus_tag="LP859_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030598.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            3313..3735
                     /locus_tag="LP859_002719"
     CDS             3313..3735
                     /locus_tag="LP859_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030599.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            3755..>4485
                     /locus_tag="LP859_002720"
     CDS             3755..>4485
                     /locus_tag="LP859_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030600.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNT"
BASE COUNT         1745 a          630 c          880 g         1230 t
ORIGIN      
        1 agacgatgat caccaacatg atgattacgg aagttactct tttacattag gtgacgtgca
       61 aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
      121 tacaatcggc aatcctcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac
      181 tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca gactttagtt atttcactcg
      241 ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca acgttttaaa aaacgaaaaa
      301 gttatcttag atggctgggg atctttatga ttctcggctt tttgattctt gctagtccga
      361 tttacacaca taaaaaggta gacataccag atgaagtaag cacgaacgca tttttaaaca
      421 cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
      481 ttgcgatcga cgatgaagat cagagcttat taaaagaact agctaatctt aattatcgtg
      541 tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg atcaaggtgt
      601 ctgatgatta ttttgtagtg aagttagcac atgtaccaga agaatatatt gctatgcgat
      661 taactattat tcctgaaaaa attgatccaa aggtggatat gcaagaaccg caagatctga
      721 ttttttatat tcacgaagat aaggtcaaag atcacgtgaa agacgaagat tatgaacaac
      781 atgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagca caaaaacaaa
      841 ttgaatcctt ccaagcaacc attgatttga atgaagaatt agtgaagaaa ctaaaagatc
      901 aattgcctta tcaagtcgca gaagatcagg aaaatactga aaataagatt aatagttacc
      961 agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagagc attcaaaaac
     1021 ttcaagaaaa aattgatcgt atgacaaaag aaaatattta gaagaatagg tggagtaaaa
     1081 gaaatgaagc gagcgaactt ggtcggcaaa gatactttcg ctgagcagaa agtattgatt
     1141 aaagcctatc tcaaagttgt aaaagctaaa gaaatatgtc aagaagtgaa ttatctaaca
     1201 caacaatatc tgagttgaag aatggtagaa agagtttaga taccttaaaa ttcagatcat
     1261 ttgaagctct atatatagct gctaaaaaat attttacagc tgataataaa taaaaaatta
     1321 aggtgggttc tatgtgaaaa aaacgtatca aaagttttta tggattagct atatcgtcct
     1381 atttatcgtt gggtttctcg tcagtgtgtc cgtgatcgtc ggaacgcttt ttcttttgtt
     1441 ctctgacgaa gatctggtac ttaaggggtt aatgagtacg ttatcgtgtg tcatgttact
     1501 ttatttatgt atacagatca acgaaattat gccccctcat tggcaagaaa agaagaaaga
     1561 atttaaggaa gaaatcgaaa taaaatccaa tgactggatt gaatacacat ggcttcctct
     1621 gaaaagaaat agcaaacgaa aggaattttg ggcattgcaa ttatggccaa taggaaaact
     1681 ttttatttat atggcttcac ttatggttac tttaatcggt tatttggtgg tggtctatgg
     1741 ggtggcctat tacttgttaa gtgagccaaa acagatcgcg gcgtcaaata ttttcacggc
     1801 agtaatgaca ttaatgtgtg tcatgtgctt ttctattttt agttacttac tcgtccaatt
     1861 tatcaggcaa aatttaacaa tgagtaaact tttcaaaagt ttattggact tacttatctt
     1921 aggcagtatg ttaacgactc taacagatcg gttcgaacaa tctgaaaaaa cggtagaggg
     1981 gataaagaac ttcttaacga cgattgatca atttataaat ggatatatgt atttgtttga
     2041 agtaggcttt attctcactt tgtttggttg tgtatttata aaactcatta aaagatcaat
     2101 cgaaaaagaa atggttattg atctaattcc gacgctgagc ggctatgagt attacgttga
     2161 ttttggaaag tatgaggagt tgatccatac ggatatcgtt cacacgagta tccatgaagt
     2221 aaccgttaag ggcatcagat ctactttaaa agatggacag gagacgaaaa caatcgaccg
     2281 aagaaaagag gtctacaatt tttataaagt tgaagcaagt ctacgatatg gcattgggaa
     2341 attgaggtta gatgggagta atattcaaca aaacaaacca tatgttttag gaaaagtggt
     2401 gatttagaac ttcctatgaa agatgaataa ataaggagaa tcttttgaaa atgattcttc
     2461 tttacagaat tgtgcttata gttggcacgg agttaaaaat cttaaagaag gagcagttat
     2521 tcaaagtgaa agaacatcat aatcagttac tatgtattaa aacgggtacg atgaacattc
     2581 aataccgaac agaatattta gaattaacga atcaaactga ttttttgtcg attcgagagc
     2641 aacataagca agaaaaatta atggctactt atagccaatt gattgatgag tgggaacaat
     2701 tcaaggaatc taaaacataa aattccattt aaacacctta ttttttaaag agcataagga
     2761 aacgtgtacc cactttcaaa ataaagtggg tagataggct gtgcctatca cgtttctgaa
     2821 aaaaataaat gaccccaaaa ttttcaattt atgtgtaggt ttatgggtag caaaaatatg
     2881 aatgtgggca gcaaaaagtt catttgtggg tagtaaaatt caaatgtgtg tagcaatgtg
     2941 tgtagctaca cacattgcta cacacgctat aacatatagt gaaaaggaga tatttatgga
     3001 agtaaaaata agagatattg atgaaaaaac gattcaaaga attgaccgaa gatgtattga
     3061 attaagtgaa cgcaccggga ctaaatggag ccgaaatcga tacctaaaag ttttgattga
     3121 aaatgatttt gaccatgaac ttatggatca tagaaaagat caatttgatc ggttgttaga
     3181 aaaatttgtg gatattcaaa cttataatac gaaaatttta gaagaatata ttgcaacaaa
     3241 taatcaatta atcgccttgt taattgaata ataaaaatag tattgtgaat ttataaaagg
     3301 aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat atctttacga tatgcatatc
     3361 gaagtacttg atgaaataat ttctactcat gaagatatat ctaattatag tgaagcagtc
     3421 agacatttgt gtatgatgta tgatccaaaa atcgaagaaa aaataaatcc aattcaaatt
     3481 aaattgaatg caatgggaaa agaaatttct atattaaccg aaatggtttc agagtttgcg
     3541 gatcttcatt tgaaagatac aggaatacta attggtacaa attcaactgt ctatcaagaa
     3601 gcaaaagagc gtgtggaagg gaaaataaaa gcgaatcaaa cgaaaaagta ttctcctaag
     3661 atcaagaaac caataaatga atctgttcat gccagagtag acccgtttga tccaacaaag
     3721 tttttaaaaa gataaaaagt agaaggggaa catgatgaat aaatcgcctg cagtcattct
     3781 cacttgtcaa tttaccttgc ccaataaaaa atctttttca acatacattg attatatgac
     3841 aagagaaaaa gcgctggaag aaaaggaacg acgcacgccg gacgaagaac aggaactgca
     3901 acgaatcaag aatgctttag aggattttta tattcctgaa ggagaaactt tttcggagag
     3961 taagacagat aaagaattca gtgaaacgtc gaatgaagct caatcgttga tggaacatgg
     4021 aatcaatttt gacaagttag aggaacaaga ttttaccaaa tatctttctt atatgacccg
     4081 tcactatgcg ttagaacaaa agaaagaact gacgcaaagg gaacaaaaag aataccaacg
     4141 gttgaaccaa gcaatgaaaa aatatcaaac agagccacat aaaaaagctt caggaaatct
     4201 tcctggagtt ttttctatgg cttcagacga agtgaaagga agtgatctga aggagatcaa
     4261 acaaatcttc caaagagggc aacaaaaagg ctcaatcatt tatcaagatg ttgtctctca
     4321 cgacaattcg tatcttgaaa aaatgggctt gtacgatccc aaaacagata ccttagatga
     4381 agaaggatta aaagcagctg gaagaaaaat gatggagaca ttattcgaga aagaacaatt
     4441 gaatgaaaca ggttactgga tggcaacgat tcaccgtaac actaa
//
LOCUS       DAFEZB010000127         4433 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFEZB010000127
VERSION     DAFEZB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.130"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(543..2048)
                     /locus_tag="LP859_002721"
     CDS             complement(543..2048)
                     /locus_tag="LP859_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3030601.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LP859_002722"
     CDS             complement(2045..2731)
                     /locus_tag="LP859_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3030602.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LP859_002723"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LP859_002723"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL3030603.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAFEZB010000128         4374 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFEZB010000128
VERSION     DAFEZB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.131"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(83..1051)
                     /locus_tag="LP859_002724"
     CDS             complement(83..1051)
                     /locus_tag="LP859_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3030604.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="LP859_002725"
     CDS             complement(1498..2904)
                     /locus_tag="LP859_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL3030605.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="LP859_002726"
     CDS             complement(3126..3488)
                     /locus_tag="LP859_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030606.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3830)
                     /locus_tag="LP859_002727"
     CDS             complement(3492..3830)
                     /locus_tag="LP859_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030607.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3809..4228)
                     /locus_tag="LP859_002728"
     CDS             complement(3809..4228)
                     /locus_tag="LP859_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030608.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="LP859_002729"
     CDS             complement(4221..4361)
                     /locus_tag="LP859_002729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030609.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DAFEZB010000129         4157 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFEZB010000129
VERSION     DAFEZB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.135"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            179..775
                     /locus_tag="LP859_002730"
     CDS             179..775
                     /locus_tag="LP859_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL3030610.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="LP859_002731"
     CDS             899..2698
                     /locus_tag="LP859_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL3030611.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="LP859_002732"
     CDS             complement(2976..3188)
                     /locus_tag="LP859_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030612.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="LP859_002733"
     CDS             3650..3799
                     /locus_tag="LP859_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030613.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4052..>4157)
                     /locus_tag="LP859_002734"
     CDS             complement(4052..>4157)
                     /locus_tag="LP859_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3030614.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1227 a          708 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacacaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DAFEZB010000130         4034 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFEZB010000130
VERSION     DAFEZB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.136"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            73..234
                     /locus_tag="LP859_002735"
     CDS             73..234
                     /locus_tag="LP859_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL3030615.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="LP859_002736"
     CDS             253..1530
                     /locus_tag="LP859_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3030616.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="LP859_002737"
     CDS             1540..2196
                     /locus_tag="LP859_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030617.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="LP859_002738"
     CDS             2196..3572
                     /locus_tag="LP859_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3030618.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="LP859_002739"
     CDS             3702..>4034
                     /locus_tag="LP859_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3030619.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          439 c          704 g         1279 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaattaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAFEZB010000131         3891 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFEZB010000131
VERSION     DAFEZB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.137"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..96
                     /locus_tag="LP859_002740"
     CDS             <1..96
                     /locus_tag="LP859_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL3030620.1"
                     /translation="PKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            149..2125
                     /locus_tag="LP859_002741"
     CDS             149..2125
                     /locus_tag="LP859_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030621.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVTSETSPYSVFKWGDTA
                     GQGLSFVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYFGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            2140..2892
                     /locus_tag="LP859_002742"
     CDS             2140..2892
                     /locus_tag="LP859_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3030622.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAAGVLLLMPAEASVAKYQQNQ
                     QIVAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEKN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2908..3669
                     /locus_tag="LP859_002743"
     CDS             2908..3669
                     /locus_tag="LP859_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030623.1"
                     /translation="MRNRKWLFVCAAALLSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKNLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIGQQT
                     GVFEFSVEVQANQKQAYYIVNETSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3682..>3891
                     /locus_tag="LP859_002744"
     CDS             3682..>3891
                     /locus_tag="LP859_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030624.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQPYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIV"
BASE COUNT         1420 a          639 c          804 g         1028 t
ORIGIN      
        1 ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga
       61 aaagaaagat acgtggtaca gggagagcta caataacaag gttttaatca acgcgagtcg
      121 ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg
      181 aatgcttgtt ggaatgttag gaatcattgc aagctgcttt caggggtatc aagcacatgc
      241 agaagatgtc acgcaaaaaa cgccaccaga gaaggtcaat atcacggttc ataaattaat
      301 gtacgatcaa ggaacgcaat tgaatgttga tattgacggg atcaaaaacg atggttatac
      361 gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga
      421 gtttacgtta ggaaatatta cggatcaagt attaccgact gaagattctg atctaaccaa
      481 cgcgaaagtg gacgaaatcg ttaaagatgt agaagacaag ggatctaaca gtgagtacgt
      541 caagaatgca accaataaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttgc
      601 ggatcaacca gcctatgtca attccaaagg gaatgtatat gtggtgtatg aaagtaaatc
      661 tgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga
      721 taatacaggt tcaggttttt taaaagacat tcacttatat cctaaaaata ttgtttctaa
      781 gttgagtttt gaattaacga aatttggtga cgatggcaca gctcaaagca aacaaacacc
      841 tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattagg
      901 agatcttgta tcggatgatc aaggaaagct gactgcgact gatttgacgt taggaaaata
      961 ctattttgtg gaagttcctt cagaagttgt agtgggatca gataaagaac caacagcaga
     1021 tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac aataaattaa cctttgaaat
     1081 tacgaatgac ggtgtgacaa gcgatttaaa agcttcatat gtgaactata aagcaccagt
     1141 gatcgataaa acagtgacaa atggaacagg acaagaacat tctttccaaa ttggtgacgc
     1201 agtgaattat caaggtacaa ttcatatccc tacagatatc gcaggtggcg cagacggtat
     1261 cacagtaaac ggtgtgacaa gcgaaacttc tccttacagt gtatttaaat ggggggatac
     1321 agcaggacaa ggactttctt ttgtcgctgc taaagcaaat atcaaagtaa caaataaaga
     1381 tggatctgtg gtattaaaag aaaatacaga ttataaaatc caaaattcag aaaatggctt
     1441 tgtgatcgat tttatcgtga ataatggcca agtaagtgat actgtagcaa gtttacacgg
     1501 acaagatctt caaatgactt ataacatgta tgtaaatgac agcgcagctg ttgccaatcc
     1561 attaacaaat agcgtggatt tcgtttataa caacaatcca tttaatcagg aagaacacca
     1621 cgaaaaaacg aaagctgacg ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg
     1681 actatttgga acaggaatca aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa
     1741 cgcagaagga aaatattttg gtggattagt tgatacggat aaagacggtg tcaaagaagc
     1801 agtatgggta gacgatgtag caaatgcagc gatcctaaaa tcagataaag aaggtcactt
     1861 tgaaatcacc ggtttaactg aaggtgaata ttcactggaa gaaacaaaag cgcctgaaaa
     1921 ctatcaaaaa ttaacgaaag agatctcttt caaagtagac aaagattcat acaaagaaga
     1981 aaaccgaatc acgatcaaaa acaatcaaaa agcttccgta ccaatgactg gtagtaacgg
     2041 tttccaaacg tatgtattga tctcttgctt gttgctagga gcaggggcac tttctgcagt
     2101 tgtttacttt aagaagaaag cctagaagga tcaaagccaa tgattatcag gaagcctaag
     2161 aaaaagcgaa tcatgggtaa atggatcatt gctttctggt tactttcagc tgcaggtgtc
     2221 ttacttttga tgcctgctga agcgagcgtg gcgaaatacc aacaaaatca gcagattgtc
     2281 gcaatcgatc gaacaggaac ggcagcagaa acggattctt ctttggatgt tgctaagatt
     2341 gagttaggcg atcccgttgg tattcttaca attccaagta tttctttaaa actaccaatt
     2401 tatgacggaa cgtccgacaa aattttagaa aatggtgtag gtattacgga aggaactggt
     2461 gatattacag gaggaaacgg taaaaatcct ttaatcgcag gtcatagtgg actttataaa
     2521 gataatcttt ttgatgattt accttcagta aaaaaaggag aaaagtttta tatcaaagtt
     2581 gatggtgagc agcacgctta tcaaatcgat cggattgagg aagtgcaaaa agacgaacta
     2641 caaagaaact ttgtcactta cttagaacca aatcccaatg aagatcgagt aacgctaatg
     2701 acatgtacgc ctaagggaat caacacacac cgtttcctag tgtatgggaa acgagtaacc
     2761 tttacaaaat cagaattaaa agatgaaaaa aataagaaac aaaaattatc ttggaaatgg
     2821 cttttaggaa gcactgtttt tctttcagtc atgatcattg gttcgctatt tgtttataaa
     2881 aagaaaaaat aaaggagggt aaccaacatg agaaatagaa aatggctatt tgtttgtgca
     2941 gccgctcttc tttctttcac tgcctacaca cagttgacgt ttgcagacag tcaagaaagc
     3001 acggatagca cagaacaaac aatccaaacg tcagatagca gcacgattca aagcagcgat
     3061 gaaaccgtcg attcgtcaac agttgattct tcagaagaac ctaaaattgc tgaagacgat
     3121 caaatagtca aacagaccat tcacgttcaa aagattattt cttccgaaca attagacaac
     3181 caggggcaaa agcttgaaaa acaagaagga gtaaatggcg cgaagttttc tgtttatgat
     3241 gtatcggata ttttacaaaa gatggatgtt aagaatctca ctactgacca aattgaatcc
     3301 caattgaaag atcgagtgaa aaaattatcc agtgatcaat tgaagctagt ttctaatgga
     3361 gaaacaaaga caattggtca acaaacaggt gtctttgaat tctcggtaga agtacaggca
     3421 aatcagaaac aagcttatta tattgtcaat gaaacaagtc ctgaaaacat ttctaatagc
     3481 gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt aaaggatgtg
     3541 tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtgaaaaa aatcgtttct
     3601 actggcgtaa agaaaaattt ctttgaaaat tgttgggatt ttgtgactca cttgttttct
     3661 agagattaag gaagaaataa gatgaaactg atcaaattat tattattcaa tctttcttgt
     3721 ttaaccgcag tctttttgta ctgcggttta ttattttctc aattatctat tttaccaagt
     3781 taccaaccat acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt
     3841 atggaaaagc tcattaggaa tccactattt actacgatca atttgatcgt t
//
LOCUS       DAFEZB010000132         3889 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFEZB010000132
VERSION     DAFEZB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.138"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..96
                     /locus_tag="LP859_002745"
     CDS             <1..96
                     /locus_tag="LP859_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL3030625.1"
                     /translation="PKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            149..2125
                     /locus_tag="LP859_002746"
     CDS             149..2125
                     /locus_tag="LP859_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030626.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            2138..2890
                     /locus_tag="LP859_002747"
     CDS             2138..2890
                     /locus_tag="LP859_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3030627.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2906..3667
                     /locus_tag="LP859_002748"
     CDS             2906..3667
                     /locus_tag="LP859_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030628.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3680..>3889
                     /locus_tag="LP859_002749"
     CDS             3680..>3889
                     /locus_tag="LP859_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030629.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIV"
BASE COUNT         1415 a          632 c          802 g         1040 t
ORIGIN      
        1 ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga
       61 aaagaaagat acgtggtaca gggagagcta caataacaag aatttaatca acgcgagtcg
      121 ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg
      181 aatgattgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc gagcttatgc
      241 agaagatgtc acgcaaaaaa cgccaccaga aagggtcaat atcacggttc ataaattaat
      301 gtacgatcaa agtacgcaat tgaacgttga tattgacgga atcaaaaatg atggttatac
      361 gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga
      421 gtttacgtta ggaaatatta cggatcaagt attaccgact gaagattctg atctaaccaa
      481 cgcgaaagtg gacgaaatcg ttaaagatgt agaagacaag ggatctaaca gtgagtacgt
      541 taagaatgca accaataaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttgc
      601 tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg aaagtaaatc
      661 cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga
      721 caacacagga tcaggatttt taaaagatat tcatttgtat ccaaagaaca ttgtttctaa
      781 gttgagtttt gaattaacga aatttggtga cgatggcaca gctcaaagca aacaaacacc
      841 tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattagg
      901 agagcttgta tcggatgatc aaggaaagct gactgcaact gatttgacgt taggaaaata
      961 ctactttgtg gaagttcctt cagaagttgt agttggatca gatcaagaac caacagcaga
     1021 ccaatatttg ttaggcgcag acgctcgcaa cgatgcgcac aataagttaa cctttgaaat
     1081 tacgaatgac ggcgtgacaa gtgatttaaa agcttcctac gtgaactata aagcaccagt
     1141 gatcgataaa acagtgacaa atggaacagg agaagaacat tctttccaaa ttggtgacgc
     1201 agtgaattat caaggtacaa ttcatatccc tacagatatc gcaggtggaa cagatgggat
     1261 tacagtaaat ggtgtaaaaa gtgaaacttc tccttacagt gtatttaaat ggggcgacac
     1321 agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa caaataatga
     1381 tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg atagtggttt
     1441 tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga atttacatgg
     1501 tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa tcgcaaatcc
     1561 attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg aagaacacca
     1621 cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag tcgatagcgg
     1681 cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg cagctaagaa
     1741 tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg tcaaagaagc
     1801 agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag agggtcattt
     1861 tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag ctcctgaaaa
     1921 ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat acaaagaaga
     1981 aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg gtagcaacgg
     2041 tttccaatct tacgtattga tctcttgctt gttgcttggg gcaggcgcaa tttctgcagt
     2101 tgtttacttt aagaagaaag cctagaagga tcagctaatg attatcagac accctaagaa
     2161 aaagcgaatc atgggtaaat ggatcattgc tttctggtta ctttcagctg ttggtgtctt
     2221 acttttgatg cctgctgaag cgagcgtggc gaaataccaa caaaatcagc agattgccgc
     2281 aatcgatcga acaggaacgg cagcagaaac ggattcttct ttggatgttg ctaagattga
     2341 gttaggcgat cctgttggta ttcttacaat tccaagtatt tctttaaaac taccaattta
     2401 tgacggaact tccgacaaaa ttttagagaa tggtgtaggg attacggaag gaactggcga
     2461 tattacagga ggaaacggta aaaatccttt aatcgcaggg cacagtggac tttataaaga
     2521 taatcttttc gatgatttgc cttcagttaa aaagggagaa aagttttata tcaaagttga
     2581 tggtgagcag cacgcttatc aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca
     2641 aagaaacttt gtcacttact tagaaccaaa tcccaatgaa gatcgagtaa cgctaatgac
     2701 atgtacgcct aaggggatca acacacaccg tttcctagtg tatgggaagc gagtaacctt
     2761 tacaaaatca gaattaaaag atgaagaaaa taagaaacaa aaattatctt ggaaatggct
     2821 tttaggaagt actgtttttc tttcagtcat gatcattggt tcgctatttg tttataaaaa
     2881 gaaaaaataa aggagggtaa ccaacatgag aaatagaaaa tggctatttg tttgtgcagc
     2941 cgctcttttt tctttcactg cctacacaca gttgacgttt gcagacagac aagaaagcac
     3001 ggatagcaca gaacaaacaa tccaaacgtc agatagcagc acgattcaaa gcagcgatga
     3061 aaccgtcgat tcgtcaacag ttgattcttc agaagaacct aaaattgctg aagacgatca
     3121 aatagtcaaa cagaccattc acgttcaaaa aattatttct tccgaacaat tagacaacca
     3181 ggggcaaaag cttgaaaaac aagaaggagt aaatggcgcg aagttttctg tttatgatgt
     3241 atcggacatt ttacaaaaga tggatgttaa ggatctcact actgaccaaa ttgaatccca
     3301 attaaaagat cgagtgaaaa aactatccag tgatcaactg aagctaattt ctaatggaga
     3361 aacaaagaca attgatcaac aaacaggtgt ctttgaattc tcggtagaag tacaggcaaa
     3421 tcagaaacaa gcttattata ttgtcaatga atcaagtcct gaaaacattt ctaatagcga
     3481 ggatattttg ctattaacac ctgtttctga taaaaatggt gaattcttaa aggatgtgtg
     3541 gatttatcct aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa tcgtatctac
     3601 tggcgtaaag aaaaacttct ttgagaattg ttgggatttt gttactcact tgttttctag
     3661 agattaagga agaaataaaa tgaaactgat caaattatta ttattcaatt tttcttgttt
     3721 aaccgcagtc tttttgtact gcggtttatt attttctaaa ctatctattt tgccaagcta
     3781 ccaactatac ctaatgagga ttttaaacat accagctgct tccatgccgc tagatattat
     3841 ggaaaagctc attaggaatc cattatttac tacgatcaat ttgatcgtt
//
LOCUS       DAFEZB010000133         3878 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFEZB010000133
VERSION     DAFEZB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.139"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(718..3867)
                     /locus_tag="LP859_002750"
     CDS             complement(718..3867)
                     /locus_tag="LP859_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL3030630.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAFEZB010000134         3848 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFEZB010000134
VERSION     DAFEZB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.140"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            108..2459
                     /locus_tag="LP859_002751"
     CDS             108..2459
                     /locus_tag="LP859_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL3030631.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2539..2934
                     /locus_tag="LP859_002752"
     CDS             2539..2934
                     /locus_tag="LP859_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030632.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3259..>3848
                     /locus_tag="LP859_002753"
     CDS             3259..>3848
                     /locus_tag="LP859_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3030633.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKE"
BASE COUNT         1354 a          494 c          775 g         1225 t
ORIGIN      
        1 atttctttgt gctgttttat tatttttagt tgttgacaaa cactaaaata taaaatataa
       61 tcaacacata aacgaacgta tgttcggcaa gaaagcaggg gatttttatg gatgagatgg
      121 ttttaagtac tcagaaatgg ctaaacaaga aatacagtaa tgttacaggg tttgataagg
      181 ttccagaaaa tgggcgaaca gggtggccta caatctatgg acttattgaa ggactacaag
      241 tagaacttgg tattactaat ctagtagcta attttggacc taccactgaa aaaatgtacg
      301 ataatcaggt tactcctaaa tggggcaaaa atctacctaa aaatattgta tttcttattc
      361 aaggtgcctt ttggtgtaag gggattaatc caggtggctt cgatggggta tatactccat
      421 atttagatac tgctgtaaaa gagttgcaga ctgatgcagg gtttaatgga agcgctgtaa
      481 ctggagtttt ggatgcgaaa tgggccaaag cgttatttga tatgagtgct tttgttcttg
      541 ttcccggcgg agatagtaaa attagaaaaa tgcagcaatg gttaaatgta aattatttag
      601 aatacacggg aataatgcct tgtgatggta tttatcaaag aaattcaaat caagcattaa
      661 tttttgcatt acaagctgaa ctaggatatt ctcccagtga agcaacaggg tcttatggta
      721 atgggacaac tagtaaaact tatcctgtgt ctgaaggtaa ctcgggaaac tatgtaagaa
      781 taattcaata tggattatat gttaatggtt attttgaaga cggtacattc gatggtattt
      841 tcactaaata tatgggttta gaagtactgg cattccgaaa atttatggta ttgcctgaat
      901 atactgaaat agcagatgtt gttgtaataa aaggactatt gtctagcgct ggagatatta
      961 gaagatctgc agatggcgca gatacttcta cgcaattaac tagatctcaa atccaaacat
     1021 tggttgataa tgatataaaa atagttggta gatatcttac tggtactgtt ggaattggaa
     1081 aagatgaacg aaataagtat ctaactacag aggaattgaa taatattttt tctaaaaatt
     1141 tatctgtgtt tcctatctat caagatggag gtgcagcatt agcttatttt acttatgatc
     1201 gtggcttatc agatggtaag aaggctatag atgcagcaaa aaatctaaat ataccattaa
     1261 caacagttat ttattttgct gttgatctag acatgttagg ggaagaaatt cttgcatatg
     1321 ctggagaata ttttaagggg gtatctgctg taatgtcata tagcgggtat caaacgggtg
     1381 tttacggaac aagaaatgtt tgttctcaaa taatcaataa cgggtatgct tcgtttgcat
     1441 ttgtttcaga tatgtcgact ggttatagtg ggaatttagg ttttcctatg cctagacaat
     1501 ggtcttttga tcaatttgtt gaatttacca taggaagtgg caacggagct gttggtatcg
     1561 atttaatcgc tacatcgggt agagatagcg gatttaatga gttatcaaat gatactaata
     1621 atgataatta cattgccaaa tacaatcaga aggttataaa tcagatggta cgtgcgtatc
     1681 aatatctatc acaagctggt cttgataatc cttggaaccc tcatttgaca ttttatagat
     1741 atgtaaatta ttctggtttg tcatgggaca tcatttcttc tcctgtaaca gagcatgata
     1801 gaaaaattta tgatgaatat aggaataaat taactaatag cgagggcctg tataattatt
     1861 ttatagatcc taatactggt tcaataatag gtttgcctca cttaattgtg acgttacaat
     1921 cacagatgtt tctagccgac acaatcaatg atagtgtttc tgactttgca ggttggttag
     1981 gagatttaat gacttgctgg ggtgaagtta agaaattagg aatacaaatg gagcaaggtg
     2041 tatttgcatt agtaggtacg gctgcaggtg gaacatttag tctagaagat ttataccaag
     2101 atgttgacgc ggtaaatgtt tataccttag caaaaagaaa ttctgattca acagctttat
     2161 cggctttaaa aacgtactat tgtgataagg gtgctattga tagatttaat caatttgtta
     2221 agaacagatt tggttcttca gataaaatcg cttcagtaac aaatagttta ttagaaaatg
     2281 acgaaacagt tgatatctta ggtgtaggga taatttcatt tttcattgat aaaatagtta
     2341 ataattcact tgggacagat tttacttatc ttgattttat tggagatgag gggaaagttg
     2401 caaatggatt ttctgaaaaa attattcaat ttcaagaaaa tagaagtaaa cttaattaag
     2461 aatcgtttac tatttaaaca gtgtcctttt cctttttaaa tatatacgat aaaataagta
     2521 tatgaattgt aaaaggacat gatgattatg gaagaaatag aatggaataa tctgactcaa
     2581 aataataaaa ataattctgg atgtggaatt atctttatgt ttgttatagt tttgggaata
     2641 gtagtacttt taataactgt caagatagta gattactttg cgaatactcc tgaagaacaa
     2701 atacagagaa aacttcctga gtttgaaaga gtaccacctt atgatccaag agataattca
     2761 tttcaagtaa aaaaattaaa agagtctcca aatgaaatgt ggtttgaagt tacagttgaa
     2821 tcaagcgact tcaaagaaaa aaagaaagaa tacaattata ttgtaaaggc aaaagtatca
     2881 aaaagattta tttttaaccc taaattagaa atatatagtg ttgtaataga ataatagtgt
     2941 taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt gaaaaataaa atataccatg
     3001 gaagacataa aagaaaaact ggataactag tttatttaat ggcagaccgt taaggcctgc
     3061 catttttatg tattttagcg gaataaatat aatcatgcgg gagtgtaaac ttactgtttc
     3121 aaaaagtata ctttttgaca tgccttgatt ttctttgttt atgtatggca taataaccac
     3181 ataatacaat ttgaaacgtt tctgatttaa tctgattgtt ttgaaacaat tttagtgaaa
     3241 aaagggtaga taaaaacgat gagaaaattt ttttatatcc gggtcagtag ttatgatcaa
     3301 tcgatcgaac gacaactgat ttccgcgaag gaattaggaa tccctgaaga atatatattt
     3361 gtagaaaaag ccagcggcaa agattttaaa cgccctgaat atcaattaat gaaacggatg
     3421 tttcgaaaag gagatttact ctatctacaa tcattagata gactaggcag aaataaacaa
     3481 atgattttag aagaatggcg agaattaact aaggaaaagc agattgatat ttctgtgtta
     3541 gacatgcctc tgttagatac tgctagttac aagcaactag atgggttaga gacgcttatc
     3601 tctgacttaa ttctgcaatt actcagctat atggccgaag acgagcggaa acgaattaat
     3661 agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg gtgtaaaatt cggcagaaag
     3721 aaaattgctt tacctcctga atttcctgcg atctattctg actggaaagc aggaaagata
     3781 actgcagtct atgctatgga tcaattgggc atgaaacgaa atacctttta tcgacgggtg
     3841 aaagaata
//
LOCUS       DAFEZB010000135         3810 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFEZB010000135
VERSION     DAFEZB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.141"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            97..2196
                     /locus_tag="LP859_002754"
     CDS             97..2196
                     /locus_tag="LP859_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL3030634.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2392..3255
                     /locus_tag="LP859_002755"
     CDS             2392..3255
                     /locus_tag="LP859_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL3030635.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3360..>3810
                     /locus_tag="LP859_002756"
     CDS             3360..>3810
                     /locus_tag="LP859_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL3030636.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1522 a          636 c          772 g          880 t
ORIGIN      
        1 ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg
       61 tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga
      121 tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa
      181 gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa
      241 tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa
      301 ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac
      361 gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca
      421 actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa
      481 caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata
      541 ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg
      601 ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa
      661 gaaactgaaa aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa
      721 gacgtaaaaa cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag
      781 gaaaaaacga ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa
      841 aagtcaacag agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc
      901 aaagagggta ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt
      961 gaagcagaag cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca
     1021 aacttagaaa aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat
     1081 ctcgttttag cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat
     1141 caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt
     1201 catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg
     1261 attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga
     1321 tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa
     1381 gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag
     1441 ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg
     1501 attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa
     1561 aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac
     1621 aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat
     1681 gaacaattga aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa
     1741 aagaaaaaag cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa
     1801 gcagcagctg cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa
     1861 gcggcatcct cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga
     1921 gcaagtgata ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc
     1981 aaagtgattg ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa
     2041 aaagccagaa tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct
     2101 gcattgatga aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt
     2161 aaaactggat ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt
     2221 gagcctgccg tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata
     2281 ctcctttttt cacctttcca aaccaaggct cagctattca agtagataac attatgttat
     2341 gatagcaaag aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat
     2401 ttattagcaa ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc
     2461 caaaaaaacg gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa
     2521 gcagttgaag gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt
     2581 cctaaaagcc gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta
     2641 ggtgcgtttg tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg
     2701 ccgacaatga cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt
     2761 gatgcaaaag accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc
     2821 aaatatggaa cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa
     2881 ttaacaggta cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa
     2941 cgatataatg aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca
     3001 gacggtgtac tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca
     3061 caaatgattt taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa
     3121 tcggatccag aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca
     3181 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
     3241 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
     3301 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
     3361 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
     3421 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
     3481 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
     3541 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
     3601 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
     3661 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
     3721 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
     3781 atggaatctg tcaggaatgc tatgaaaaag
//
LOCUS       DAFEZB010000136         3785 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFEZB010000136
VERSION     DAFEZB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.142"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..171
                     /locus_tag="LP859_002757"
     CDS             <1..171
                     /locus_tag="LP859_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL3030637.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="LP859_002758"
     CDS             406..642
                     /locus_tag="LP859_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030638.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="LP859_002759"
     CDS             649..2601
                     /locus_tag="LP859_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBL3030639.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="LP859_002760"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="LP859_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBL3030640.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="LP859_002761"
     CDS             3400..>3785
                     /locus_tag="LP859_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL3030641.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAFEZB010000137         3728 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFEZB010000137
VERSION     DAFEZB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.143"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..559
                     /locus_tag="LP859_002762"
     CDS             <1..559
                     /locus_tag="LP859_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL3030642.1"
                     /translation="THFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLT
                     IPLQKLLNRAEETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDT
                     RAGMRDQLTQFADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPE
                     DEQLLGSLVQAISYTNAKNYFGFFA"
     gene            718..1401
                     /locus_tag="LP859_002763"
     CDS             718..1401
                     /locus_tag="LP859_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL3030643.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            1403..2239
                     /locus_tag="LP859_002764"
     CDS             1403..2239
                     /locus_tag="LP859_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL3030644.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            2250..>3728
                     /locus_tag="LP859_002765"
     CDS             2250..>3728
                     /locus_tag="LP859_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3030645.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQ"
BASE COUNT         1156 a          737 c          721 g         1114 t
ORIGIN      
        1 gacccacttc tatcattctc ataattggac gatgcagctg cacatccatg cttatcgtaa
       61 ctgtaataca caaatgttgg aaaaattagg gcccgatacc ggctatgatg gaatgaatga
      121 tctttctttg actattcctc tgcaaaaatt attgaaccgt gcagaagaaa cggatcagtt
      181 gccaaagacg atcctttatt cattgaatcc gaatgactat cctgcgattt tagctttgat
      241 gggctgtttc caaaaagaac agaatggaaa actgcagtta ggttctggat ggtggtacaa
      301 cgatactcga gctggtatgc gcgatcaatt gacacaattt gctgatggta gtgctttggg
      361 gaattttgtc ggcatgctga cagattcaag aagtttcctg tcctacacta gacatgaata
      421 tttccgtcgt gttctttgtg aatttttagg cgaaatggtc atgcgaggag aagcacctga
      481 agatgaacaa cttttaggaa gtctagttca agcgattagt tatacaaatg ctaaaaatta
      541 ctttggcttt tttgcctaat tgattaatag taacagtaac gaatcaaaaa caggatagtg
      601 aaataacgga atccgtttat ccacgtttta gagaagaaga cggaccaaat ttgctccgtc
      661 ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag agataacatg
      721 cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa caagcgtccc
      781 gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca gatcaataac
      841 caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt agcccgtcta
      901 gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa cgaccagaaa
      961 atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct gcagatattt
     1021 tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt gacgcgacga
     1081 gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc aaaagcgatg
     1141 cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag atcacaagaa
     1201 ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct tatacctgat
     1261 acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc agaaggcgct
     1321 gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc tcttgctaat
     1381 tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg cccacgattt
     1441 agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact tgtcccatat
     1501 ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca tgacacttgg
     1561 gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt ccattttagg
     1621 atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat tggcaacttt
     1681 caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta cggaaaccgg
     1741 tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc agatcgccag
     1801 aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg ttgctgttga
     1861 agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa tcgatgaagt
     1921 agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata aagaaaatca
     1981 tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt atattacagc
     2041 gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac ctgttggcaa
     2101 gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc ctctaatgta
     2161 cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac acttgcagtt
     2221 tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa agttatcaaa
     2281 aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat tattggcgtt
     2341 ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta atgccgatcg
     2401 ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg caggccattt
     2461 atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg aattattttc
     2521 atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga gcgaccctta
     2581 tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct ggacgaatcc
     2641 agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa gtatttgtca
     2701 gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt tataaaagtc
     2761 ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt actctctttt
     2821 tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa gagacttctg
     2881 caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg ttagaaaaac
     2941 acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga ttccacttag
     3001 atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata tttcagctga
     3061 tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc ggcgatgtcg
     3121 tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg atcacgctga
     3181 ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg ttgattgctg
     3241 gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga aattttcgtt
     3301 ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat catttttctc
     3361 tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct ggaaaatcta
     3421 cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt tttctagatg
     3481 atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc gttgttcctc
     3541 aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc ttgaaaaacg
     3601 tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca ctgattgacc
     3661 agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg tctggtggcc
     3721 aaaagcaa
//
LOCUS       DAFEZB010000138         3720 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFEZB010000138
VERSION     DAFEZB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.144"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..184
                     /locus_tag="LP859_002766"
     CDS             <1..184
                     /locus_tag="LP859_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL3030646.1"
                     /translation="GVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQCPTCGKKYT
                     LSYGDYILIDEKGKKR"
     gene            184..435
                     /locus_tag="LP859_002767"
     CDS             184..435
                     /locus_tag="LP859_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030647.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            451..867
                     /locus_tag="LP859_002768"
     CDS             451..867
                     /locus_tag="LP859_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030648.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            849..1121
                     /locus_tag="LP859_002769"
     CDS             849..1121
                     /locus_tag="LP859_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030649.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            1288..1689
                     /gene="tnpA"
                     /locus_tag="LP859_002770"
     CDS             1288..1689
                     /gene="tnpA"
                     /locus_tag="LP859_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL3030650.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1701..2846
                     /locus_tag="LP859_002771"
     CDS             1701..2846
                     /locus_tag="LP859_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030651.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            3084..3347
                     /locus_tag="LP859_002772"
     CDS             3084..3347
                     /locus_tag="LP859_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBL3030652.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            3341..3691
                     /locus_tag="LP859_002773"
     CDS             3341..3691
                     /locus_tag="LP859_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3030653.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
BASE COUNT         1424 a          540 c          695 g         1061 t
ORIGIN      
        1 gggtgttccc ttcagcgaat tattcccaaa ctatcatgca ccacatactg tcgaatgcga
       61 ttgtggagaa agagcgaaat gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg
      121 gaaaaaatat accttgtctt atggcgatta tattttgatt gatgagaaag gaaagaaacg
      181 ttgatgaaaa cacaacacaa attattgact gtaaaagaag tcgttcaaat tttggtggac
      241 aaagaagtca tttctgaaaa agtagcagaa gctttactta ttccgcggta ctggacgaca
      301 gaaacccctt tagtagagga tgcaccgact atgctatttg aagacctagt gaagattgca
      361 ggatatgaac tgcattatca agaaaacttg gaaacttatg aaaacgaata ttggttaacc
      421 gaattagaga actaaaaagg agaaaaaaat atggctcaaa attactatga cgaatttgtc
      481 aaattacctt tggataaaat ggcacaaaaa atggaggata tgacattcct atacaatgag
      541 acacgtgttc caaaaaaaca ttataaggaa agattgtcgg tagctgtgga agaaatgatt
      601 gaatcaggtg tcgaaatgaa tctcattgcg acgtattatc gaacactgga agaactgaaa
      661 aagcaaaatg caaaatggtt tttccaagcc ttactatgct tggaagtagg cgtcaaacca
      721 agcacaatca aaccttctga ataccaagct ttagaactaa cgtatgcgaa atttatcgaa
      781 acgaaaaaag ctaagactgt ttcaagtgaa tggttagatt attttgaaaa tatcaataag
      841 tatggcgcat gttacatcat gaagtaatca gacggattca aaaagacttt agttgtatgg
      901 aagaaatgaa gtactttgtt caaaatgttg aagaacagtt atatttggaa agcgaaacga
      961 gtcaaacaaa ggtcacagtc agccataatc gtttagatgt gtataaacgt cgtcctgaat
     1021 cattaaaaaa aggagcggag cttctacgac tagaaacaga aaaatacgtc atgaatgttt
     1081 acctagaaaa aagtgagttg gtcgaaaatt ttatggacta gccgagaaca cccgtgattt
     1141 taatcatggg atgaatcgga ttcagttgtg acctagcttt agctaggtta aacaactgac
     1201 aaatataaac taaataatag tgaaatgtct tgcaataatt gattttgtag aaatacatat
     1261 attaaaataa ttgcgaggtg tttttgtatg tcagaaattc attatggacg gggttatgtc
     1321 tattctattc aatatcatct tgtttggtgt gtaaaatatc gacatgatgt tttgtatggt
     1381 caggtagata ttgatgtcaa acagtttttg aaacaaattg ctgatgataa taatataaaa
     1441 attatagaaa tagaatcaga taaagaccat attcatttac tcattgaatg caccccacaa
     1501 cactatattc ctagtattgt taaagtattt aaaggcgcat ccgctagact gctgtttaaa
     1561 aaacatcctg aacttaaaca actcctttgg ggtggacatc tttggaatcc aagctacttt
     1621 gtagctactg ttagcgaaaa tacagaagaa caaattagaa aatatataca aaatcagaaa
     1681 aagaaatgag gtgaatccgt atgctacaac atagggctta taaatttaga atctatccta
     1741 atcaagaaca agaaatatta atgactaaaa ctattggctg ttcaagattt gtctataatt
     1801 attttttgaa tttgtggaat catgaatata cgactactgg aaaaggatta tcttataatt
     1861 cttgttctgc catgcttcca caaatgaaaa gaaatgcaga gacaatttgg ttaaaagaag
     1921 ttgacagtat tgcgattcaa tcctcattga aaaatctttc tgatgctttt tctcgcttct
     1981 ttaaaaagca aaataaacga ccacaattta aaagtaaaaa acatcctata caaagttata
     2041 caactaaaaa tgtgaataat agtatcgaaa ttagaaacaa tttcgtaaaa ctacctaagt
     2101 taggatttgt taaatttgcg aaaagtcaag agcccaaagg gcgaatctta aatgctacga
     2161 ttcgaaaaaa ttcgagtggt aaattttttg tttccatcct gtgtgaagaa gaaatagttg
     2221 agtttcctaa atctaattcc gctattggaa tcgaccttgg tataactgat tttgcaatcc
     2281 tttctgatgg tcaaaagatt gataacaata aattcacgtc aaaaatggca aaaaaactca
     2341 aacgtgaaca acgtaagttg tcaaaacgtg cattactagc taaaaagaaa ggcgtcaatc
     2401 tctttgaagc taaaaattat caaaaacaaa aacgaaaagt agcacgacta catgaaaaag
     2461 taatgaatca acgcaatgac tttttaaaca agttgagcac agagattatc aaaaaccacg
     2521 atattatctg tatagaagac ttaaatacaa aaggtatgtt gcgtaatcat aaactagcaa
     2581 aaagcatttc tgatgtgtct tggtctagtt ttgtgactaa gttacaatac aaagctaact
     2641 ggtacggtcg gaaaatcatc aaagtagata aatggtttcc atctagtcaa atctgttcaa
     2701 attgtggaca caaagacggc aagaaatctc tagaaattcg agaatggact tgtcctgttt
     2761 gtcatgctca tcatgaccga gatatcaatg ctagtatcaa tattttgacc gaaggtctaa
     2821 gattacatgt catgggatta gcttagatcc aatcatcaaa aaccgtagga actacgggga
     2881 tagcttggta aataagagaa accgctgtga gtaaagaaat acgcttgcaa gtatgctcta
     2941 ttcccaagaa tctcgtgact ttagtcatga gaggttcaag gattcatgga atgttaatct
     3001 aatttaatag aaaaggataa ttaagtatat acgttttttc ttatgtatgt tatactatgt
     3061 atatacagaa agaggtgtct actatggaaa tgttacctat aaaaaaatgg ggaaatagta
     3121 atggccttag attgccaaag tatattatgg aatatcttgg aattcataca gaagacaaag
     3181 tgaagataat acaagaagaa gtaaacggtc agaagcggct aattatcgaa gcggctaata
     3241 tagaaaatga attaacaatt gaacagttat ttgaaaatta cactgatgaa agaaatcatg
     3301 tgacgattca aaatttaggt gaagcagtag gtaatgaaaa atggtgagaa aacctagaca
     3361 aggcgatatt ctattattga atacagcacc tcgatctgga cacgaacaga caggtaaaag
     3421 accttatatt gttttaagtc atgatattat tgcggattat agtaacgttg ttacagttgc
     3481 tccaatttct agtacaacta gaaactatcc gctatatgta aatataaact cagaatatga
     3541 aatgaagaca acaggaaaag tattattaga tcaactaacg acaattgatt atgaagccag
     3601 agagtgtatt tttttggaaa aagcgcatga tagtttagtt gaggagttat tgatgaaagt
     3661 tagaacagtt tttcaaaaag taagcaaata aaatagaaac agcagcatga tcaactattc
//
LOCUS       DAFEZB010000139         3419 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFEZB010000139
VERSION     DAFEZB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.148"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            164..715
                     /locus_tag="LP859_002774"
     CDS             164..715
                     /locus_tag="LP859_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBL3030654.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1391..1645
                     /locus_tag="LP859_002775"
                     /pseudo
     CDS             1391..1645
                     /locus_tag="LP859_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1715..2227
                     /locus_tag="LP859_002776"
     CDS             1715..2227
                     /locus_tag="LP859_002776"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030655.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2224..3261
                     /locus_tag="LP859_002777"
     CDS             2224..3261
                     /locus_tag="LP859_002777"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3030656.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1253 a          577 c          678 g          911 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaatt atctaaagag aattaagttt tgattatgta
     1141 ataagttttg gtactaaaaa agacttgatc taattagacc aagtcttttg ttagtgttat
     1201 attaataaca aaataaaaat aagaagtcgc tcactccccg accaaagttt gtgagcaaaa
     1261 taaacagtcg atgtttgttt cttaattgta tatcttgata ttaaacgatt taagattatt
     1321 cttcaagata tatattcgca aatgtaattg gaaaagcgac ttcttaaaca aaattaagga
     1381 gtcgcttttt atggcaaaaa taacgaaaaa tgagcaagaa aatttggtgg aaaattacac
     1441 aaaaaaaccg gcgtactcta atagccggtt agacgcacat ttcgtgtgca cttctaatcc
     1501 gaaattgagc tttgacgcta tgacgatagt cggaaacctt aataaaaaca gcgctaaaaa
     1561 gctatccgat tttatgagtt tagatccaca aattagactt tgggatatac ttcaaacaaa
     1621 atttaaagct aaagcactac aagaccccct atgttaggct agttgtcgta taggataata
     1681 agttataata aagactatac ggaggatgat taccatgaca aataaaaaca atgaacgtta
     1741 ctctaccgaa ctgaaagcga cagccatcaa gaaaatgatg ccacccgaaa atagggggat
     1801 tcgccaatta agtcaaactt tgggcattcc agaagcgact ctttgttctt ggcgtaaaaa
     1861 agctagaatt caagggaata ctacacccgg aacgaaaaaa aatgtcgaaa aatggtcaag
     1921 tgaagataaa tttttaattg tgatggaatc attctcaatg aaccaagtag aacttgcgga
     1981 atattgccga aagaagggct tatttaaaga acaaattgag gcttggaaga aaacgtgctt
     2041 atctgcgaat gaacaggaag aaaatcgaac gagagaactt gcaactgaac taaaagaaga
     2101 gaaaaaacaa gcgcgtcaat tagagaaaga attacgtcat aaggaaaaag cattagcgga
     2161 agccgctgct ctcttattac taagaaaaaa agcacacgcg atttgggggg accaagagga
     2221 agaatgattt catcttcaga tcgcaaacaa gctgttgaac ttattcaaga agccaatcgc
     2281 aatggggccc gtctcaccta tgcctgtaat gagctgaata ttagcgttcg aacctacgaa
     2341 cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc ctttggcgaa acgtcctgta
     2401 cccaaaaata aactgacgga tcaagaacgc gaaaaaataa tagaaaccgt ttcaaaaaaa
     2461 gaatttatga atttgccacc tagccaaatt gtaccaaaac ttgcggattg ctcgatttat
     2521 attgcatcag agtctagctt ctatcgtgta ttgcgagaaa aaaatatgca acatcaccgt
     2581 ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc accttgcgac gaagccaaat
     2641 gaagtttgga cttgggatat tacttggcta aaaggtccaa taaaaggact cttttaccgg
     2701 ttatatctca tcattgattt atttagccgt aaaatggtag gctgggagat ttgggaatca
     2761 gaagaagctc gtcatgccga aaaacttatt aaacagacgg tgcttaagga acgtatccaa
     2821 ggacagccgt tggttttaca ctccgataat ggcagcccta tgaaagccgc gacttttcaa
     2881 gtcttactcg aaaaattagg aattcaaagt tcttattcaa gaccacgagt gagtaatgac
     2941 aatccttatt cggaagcggc tttccgaacg ttaaaatatc gaccagaata tcccaataac
     3001 ggctttaaaa caatagatga tgctcgaaaa tggacgaaag agtttgtcga ttggtataac
     3061 gattctcatt tgcatagtgg gattcaattt gtgtctcctg cagcgtgtca tgaaggacga
     3121 catctcgcta ttttagagaa acgaaagcag gtttacgagg acgctaaaaa gcgccatcct
     3181 gaacgctggt caaaaggtgt tcgaaattgg actccacatc aatcggtggc gttgaatcct
     3241 ttaaaagaaa cagaaagtta acaggagcat aatctttcaa gattatcaaa taaccctcgt
     3301 cacccaatag gggaaaaagt tattcttccc ctattggata acgaggccaa ccgaccatag
     3361 ggaggttctg ctttttgata ggtggaaaat aaaaatacga caactatatt gacaaacac
//
LOCUS       DAFEZB010000140         3370 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFEZB010000140
VERSION     DAFEZB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.149"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LP859_002778"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LP859_002778"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL3030657.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="LP859_002779"
     CDS             complement(2261..2512)
                     /locus_tag="LP859_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL3030658.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="LP859_002780"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="LP859_002780"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL3030659.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1084 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa
//
LOCUS       DAFEZB010000141         3247 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFEZB010000141
VERSION     DAFEZB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.151"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..244
                     /locus_tag="LP859_002781"
     CDS             <1..244
                     /locus_tag="LP859_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030660.1"
                     /translation="DDNHQHDDYGSYSFTLGDVQTYAQTSSVKEEQNFVVFVDYTIGN
                     PQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            255..1061
                     /locus_tag="LP859_002782"
     CDS             255..1061
                     /locus_tag="LP859_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030661.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIISEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            1083..1313
                     /locus_tag="LP859_002783"
     CDS             1083..1313
                     /locus_tag="LP859_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030662.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            1770..2057
                     /locus_tag="LP859_002784"
     CDS             1770..2057
                     /locus_tag="LP859_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030663.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            2075..2497
                     /locus_tag="LP859_002785"
     CDS             2075..2497
                     /locus_tag="LP859_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030664.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            2517..>3247
                     /locus_tag="LP859_002786"
     CDS             2517..>3247
                     /locus_tag="LP859_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030665.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNT"
BASE COUNT         1317 a          480 c          618 g          832 t
ORIGIN      
        1 agacgataat caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca
       61 aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
      121 tacaatcggc aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac
      181 tgtcactgaa aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg
      241 ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa
      301 gttatcttca atggctggga atctttatga ttctcggctt tttgattctt gctagtccga
      361 tttatacgaa tcagaaggta gatataccag atgaggtaag cacgaacgcc tttttaaaca
      421 cgaaagagct gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
      481 ttgcgatcga cgatgaagat caaagcttgt taaaagaact ggctaatctt aattatcgcg
      541 tagaagtaaa gacagctaaa ggggattatc aagcaatacg agccaaagtg atcaaggtgt
      601 ctgatgatta ttttgcagtg aagctaacgc atgtaccaga agaatatatc gccatgcgtt
      661 taaccattat ttctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga
      721 tttattacgt gcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac
      781 acgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagct caaaaacaaa
      841 tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc
      901 aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc
      961 agcaagaaat tgaaacatcg aaaacgcaac tgaaagatca agaagagatc attcaaaaac
     1021 ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa
     1081 aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcaaaaa gtattgatta
     1141 aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaaca
     1201 caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcgt
     1261 ttgaagctct atatgtagct gctaaaaaat attttacggc tgaaaggaaa taaaaacagg
     1321 aggattttat gtgaaaaaag tatatcaaaa atgtttatgg atcagctata tcaccgtaac
     1381 acaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
     1441 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
     1501 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
     1561 ataaccttaa tttttaaaga gcataaggaa acgtgggaac actttttaaa aaaagtgttc
     1621 caataggctg tgcctattac gtttctctaa aacaaaagtg ggtacaaaat attcgattta
     1681 tggatagtgt atggatagta aaacagctat acatggatag catatggata gactccatag
     1741 actatccata cttttttcgg agggtgaaaa tggaaattaa agagattaaa gtaagaggta
     1801 ttaataaaaa atatgttcaa gaaattgatc atagatgcgt agaattaaca gaacaaactg
     1861 gtcaaaaatg gagtcgaaat gattatctaa aactattgat tgaaaacgac tttgaacgtc
     1921 ctttaatgga ctacaagaaa gatcaatttg atcgtttatt ggaaaaattt acagatgttc
     1981 agctgcataa tacaaaagtt ttggaagcat acacgaacga ggtcaagaac ttaattgaaa
     2041 tattaatcgc acattaggaa ggagataatg ttctatggtt aaagcgatag aaaagcatat
     2101 tacattttat gaagcgcacg atgaagtttt aaaacatatt atggaaactc agcctacggt
     2161 aaaaaactat tctgaagcca ttcgattttt atgtatgaat tacgatccag aaattgaaaa
     2221 aaataataat atgacgaacg caaagttgaa cgcaatggga aaggaaattt ctattttaat
     2281 agaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatat taattggtac
     2341 aaattcaact gtctatcaag aagcaaaaga gcgtgtggaa gggaaaataa aagcgaatca
     2401 aacgaagaag tattctccta aaatcaagaa atcaataaat gaatctgttc atgccagagt
     2461 agacccgttt gatccaacaa agtttttaaa aagataaaaa gtagaagggg aatatgatga
     2521 ataaatcgcc tgcagtcatt ctcacttgtc aatttacctt gcccaataaa aaatcttttt
     2581 caacatacat tgattatatg acaagagaaa aagcgctgga agaaatggaa cgacgtacgc
     2641 ctgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg
     2701 aaggagaaac tttttcggag agtaagacgg ataaagaatt cagcgaaacg tcgaatgaag
     2761 ctcaatcttt gatgggaaat ggaatcaact ttgacaagtt agaggaacaa gattttacca
     2821 aatatctttc ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa
     2881 gtgaacaaaa agaataccaa cgcttgaacc aagcaataaa aaaatatcaa acagagccac
     2941 acaaaaaagc ttcaggaaac cttcctggag ttttttctat ggcttcagac gaagtgaaag
     3001 gaagtgatct gaaggagatt aagcaaatct tccaaagagg acaacaaaaa ggctcaatca
     3061 tttatcaaga tgttgtctct catgataatt cgtatcttga aaaaatagga ttgtacgatc
     3121 ccaaaacaga tactttagat gaagaaggat taaaagcagc tggaagaaaa atgatggaga
     3181 cattattcga gaaagagcaa ctgaacgaaa caggctactg gatggcaacg attcaccgta
     3241 ataccaa
//
LOCUS       DAFEZB010000142         3199 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFEZB010000142
VERSION     DAFEZB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.152"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            301..585
                     /locus_tag="LP859_002787"
     CDS             301..585
                     /locus_tag="LP859_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030666.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="LP859_002788"
     CDS             588..1004
                     /locus_tag="LP859_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030667.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="LP859_002789"
     CDS             complement(1141..1860)
                     /locus_tag="LP859_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3030668.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="LP859_002790"
     CDS             2513..2608
                     /locus_tag="LP859_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL3030669.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="LP859_002791"
     CDS             complement(2910..3155)
                     /locus_tag="LP859_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030670.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAFEZB010000143         3162 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFEZB010000143
VERSION     DAFEZB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.153"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..554)
                     /locus_tag="LP859_002792"
     CDS             complement(<1..554)
                     /locus_tag="LP859_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL3030671.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVP"
     gene            complement(636..1802)
                     /locus_tag="LP859_002793"
     CDS             complement(636..1802)
                     /locus_tag="LP859_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL3030672.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1808..2497)
                     /locus_tag="LP859_002794"
     CDS             complement(1808..2497)
                     /locus_tag="LP859_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL3030673.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2624..2863)
                     /locus_tag="LP859_002795"
     CDS             complement(2624..2863)
                     /locus_tag="LP859_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030674.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(2930..>3162)
                     /locus_tag="LP859_002796"
     CDS             complement(2930..>3162)
                     /locus_tag="LP859_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030675.1"
                     /translation="PVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEKERL
                     RKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1003 a          622 c          458 g         1079 t
ORIGIN      
        1 tcaggaacgc catcgattaa tttaaagtac tcaggtacga taaatttacg cgttaccttg
       61 taacgataga cgccatcgtt actaactaag tccatcacct cccctaatga caacttttga
      121 atatcagaaa ataaaacacc tggcatttcc atgttatgac ctgctaggac atagtttcct
      181 ttacccattt cggcatcttc ataaaaataa gtagccgctc caagactcaa agtgtattga
      241 tttgcccctt taaaaattgg caatgctaaa ccaagtgcag gaatctgaat ttgtgcaaat
      301 acttgtttat tgacgcccat ttcgattgct tctttttgta tggacaacat ttgccccaaa
      361 gacatatcat tattgacgtt cgttgcatcc tccttataac tctttgcgtg tgcattgatt
      421 tgatctacgg gcatcttttt ttctatattt tgcttaaaca cctctccttc atgtttttgg
      481 tagctaacat agccaaacac actgcaaacg acgattaaag cgactcctaa aaaaccaaat
      541 aaccattttt tcatccatct gcctcctttc agacaaaaca aaaaacactt tttcacaacg
      601 aaaaagtgtt ttctcttatt actattctat ttcatttatc tggtggtttt atattggtgt
      661 gtagccattg ctaacattaa gaatacaatt ggcacccaac tgattgtcgt taatgtttct
      721 tccatgaacg attgacacat gatcgctaaa aaagcccaca agaatgttag ttgtccattt
      781 gtcagctcac gttttcttac gagcaaccat aagaaactaa gcaaaagaat aatcgataga
      841 acaaaaccaa tcaatccata gtgattgacg agtgcgtgat acgtattatg attacctaga
      901 atctgatttc ctctatggaa aaagaaaggt ttcatcccta caaatatttg tcctgttttt
      961 tcgcctaatg tttcatagaa tttgcgccaa atttcttctc gtcctgtaaa taagtgaact
     1021 ttgtctgaca ttgcagcaaa ataagaaatc ggtaaagcta gaaccgaaac tgctgcatac
     1081 atccaaaagg acaagtgtgt atttcgaaaa accttcttag gtaaaatttc taagataatg
     1141 aagataacaa gcgcatataa tgctgctttc gattggcaaa cccaaatcgt tgccccagca
     1201 agaatcaacg ctggtaatga tagcaatcgc atgtaccatt ttccaaatga actagcaaat
     1261 cctgtgataa gaagggctaa tgtcattaaa gaactaccta tcgtatttgt attgatccaa
     1321 atatctgata atttgttcga tggattccaa atactttgtc cttctggtac tggtatctca
     1381 gtaaatactc gataaaccgt tacgagtaaa aagacataga atactacaag atacaagcta
     1441 cgatcaatta cagtaaattt cgtccatttt acaatcaaaa taatagcaat aattgtcgga
     1501 acgatcagat caccatagaa agtatctcct cggtagcttc taaaaaaaga aaagaaaagg
     1561 taaaaaagaa tgcctgctgc aatgaagaat agatcgtttc tctttacctt gtacagattc
     1621 aaaagaaaca aacagcctac gaaaaacgtc actaactgtg cagcattatc ataaataaat
     1681 aatatcgtag gaggatttaa ccccaccgtc catattaaat atagaatgga taccaacagt
     1741 aacagaaaaa tggcactcat tttattgatc aatttttcta caagttcttg aatgtgactc
     1801 acttccctta tttattgatt ttttctacat tgactaattc acactggatc acatcgcact
     1861 ttttcatatt atttgcttga aaggctaaca gtgtgagtag tggtttttta cctgcttttg
     1921 gcaaggataa aaaggaattg ttttccttag attgaactac ctggctgtaa tcagaaattt
     1981 ttcctctttt ggagacttca tactgatact tgtaatgagc atcgattaga tcaatttctt
     2041 ctccttcaga taaatcagtc atatcggata ataaaacgct tggtgtatct acgttataac
     2101 cagcaagaat ataatttcca tttcccattt cagtatctag ataataagta gaagctactc
     2161 ctaaactaag cgtgtcgcta ttcgctcctt gaaaaacagg gagaataaat cctgtagaag
     2221 aaaaaacaag ttttccagca atttcacctt tgagatcctt ttctaatgcc tgtttttgat
     2281 tagtaatcag ttgtctctta gaaatatccg ttaaactggt taaattatct tgataggttt
     2341 tagcctgttc ctgaatatgg attgtttttg ttacaccatc tactttttca gcgattttgt
     2401 ccttttgata accttgataa gcaaaataac taaaaagaag aaaaacaatg attacgccca
     2461 cgtaaggatt aaataaagtt ttattgtttt tattcatcta tctaccttct atatatgaat
     2521 taatttacct acatctaacc aagataaacc atgttaaatt ttactattct ttttattcaa
     2581 taacaatgcc acatcataat tgatcatttt catatcataa tcgtcaagaa acttctaata
     2641 atagttggca attggaacct gtttttacat aagttcgcct caactgttca aagtttttca
     2701 ttgaacctaa caaacaagct ttctcttttc ctgttatctt catatcattg atatattgca
     2761 tgacagataa tcttttaagg tgtttttgtt tcatcatttt tagcaacttt tcttccattt
     2821 ctttcgacac tttttttctg ttttcgattt cttcataatt catgagtttt cctacctttc
     2881 ttgttttttg caaaaataaa aaagcctatg atcacatacg ctctacactt cagtcttggg
     2941 ttatcttttt ctttcgttta attactggcc gtgctggttt caaatccttg attccattat
     3001 gggttctcca ataatcgaat tcttttcgta atctttcttt ttccatatct gttcctttag
     3061 acagggattt gaatacatct ttaggtgaat aaccacgagc taacagctgt acataccttc
     3121 cttttgtcaa agtatccata tccatcgcct ctaaaactgg tc
//
LOCUS       DAFEZB010000144         3102 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFEZB010000144
VERSION     DAFEZB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.154"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..544)
                     /locus_tag="LP859_002797"
     CDS             complement(<1..544)
                     /locus_tag="LP859_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL3030676.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(567..1052)
                     /locus_tag="LP859_002798"
     CDS             complement(567..1052)
                     /locus_tag="LP859_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3030677.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(1042..1854)
                     /locus_tag="LP859_002799"
     CDS             complement(1042..1854)
                     /locus_tag="LP859_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL3030678.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(1993..>3102)
                     /locus_tag="LP859_002800"
     CDS             complement(1993..>3102)
                     /locus_tag="LP859_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL3030679.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT          960 a          669 c          554 g          919 t
ORIGIN      
        1 aataattcgc tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag
       61 cttgtccaag ggttttcaaa cgaggtctga caggtacttg gacatgtttg atatgcatac
      121 cgattgatgt gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag
      181 gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa
      241 caacagagac aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc
      301 ggttcaaatg ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt
      361 ttaacgtacg gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc
      421 caacgacttc gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct
      481 cagccaatat gtcattaact atttcagtca attggtcttt taattctttt tctgacaact
      541 tcattttcta aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga
      601 cttgcagtta taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt
      661 atagaagcca ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg
      721 atcataacta agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg
      781 aaagaaccag caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc
      841 ggatatcctg aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc
      901 gttggaccat aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca
      961 ttcgtagccg gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc
     1021 atagcataga tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc
     1081 agaccagaca cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac
     1141 aaatttttta ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt
     1201 tattgcagaa gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg
     1261 agaaaatacc tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat
     1321 ccttgtgccg cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag
     1381 atggatcatt tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc
     1441 caaattaggc gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata
     1501 caattgattg atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg
     1561 gatattttgt tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa
     1621 taacccaatt ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt
     1681 aagcaattcc aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat
     1741 agaagtcaag gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg
     1801 gattcctcca ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt
     1861 ttcctccatg aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata
     1921 tttttatctc atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct
     1981 ttccttaaat agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac
     2041 tttcattgta gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg
     2101 aggaagttca tggacttgct cccaccatcg attccaatcc atttctttat caggagctaa
     2161 agcaataatc ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa
     2221 atctaacgaa tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa
     2281 atccacttgc cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc
     2341 gctatcccac caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt
     2401 caaagcccgt cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt
     2461 cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct
     2521 tgcatcactt gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc
     2581 actttggcca tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt
     2641 cttcattgtc tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa
     2701 agcttctgga tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc
     2761 tagtgcaatt ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc
     2821 taaaggcatc ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga
     2881 atgcactgga gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt
     2941 tttttgccat ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat
     3001 accagttgta ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg
     3061 agaaacagac aaagcaggtt gttccattaa ataaagaata gg
//
LOCUS       DAFEZB010000145         3064 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFEZB010000145
VERSION     DAFEZB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.155"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            7..624
                     /locus_tag="LP859_002801"
     CDS             7..624
                     /locus_tag="LP859_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030680.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDTLKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1949..2503
                     /locus_tag="LP859_002802"
     CDS             1949..2503
                     /locus_tag="LP859_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3030681.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            2757..>3064
                     /locus_tag="LP859_002803"
     CDS             2757..>3064
                     /locus_tag="LP859_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030682.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYVLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT         1195 a          346 c          493 g         1030 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatac attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaataaaa atatagaaat aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aagaactagt agaagaagga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaattatga acttgcgtaa agttataaaa
     1561 tattaataaa aatattttat taatgaaaag cttggtagca cggttattca tatgctaatt
     1621 tatttcactt tatagtaagg ggagttctta gtatattgtt caaacttaaa tttcatataa
     1681 tttaattatg taaattagct attctaagaa cttctacgga taactgtccg tctaaaattt
     1741 aaataagagc ttagaatctt catataagta aggttaattt ttttgtacaa aaaagtcgga
     1801 atattctgag ttactgaaat gatataaaat cataaactaa accccttgat tttcttcact
     1861 tttaacagag tatatatata agttcatgat tatggtaaaa tatatatata atcatgaact
     1921 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
     1981 tgaagagata caagattttc agttttggct aagaagaaca agtaatccag aaagagatag
     2041 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
     2101 agttggagac atacgaggta aaagtaagcc tattattgtt gaacataaaa caggtaagcg
     2161 aaaaccgatt tttatagata atttgagaga agagatttta ttatatactg aaggtaaaga
     2221 agaaaatgat tggttatttc caagtcggca acaaggtcga catataacaa gggatcgtgt
     2281 ctatcagata tatgcagata tagcagaaaa attaggaaga gatgatatag gaacacatac
     2341 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
     2401 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
     2461 tgaaataggg gcatcgttac gtggttttaa attgggaggc taattgagat attaggatat
     2521 attttttgtg tataatgaaa acttagaagg tgagattaat gaagataact gaattttcgg
     2581 aagtatttaa aacgtttagt aacatttaca atagaatacc agagatagaa caaaatgttg
     2641 tagttgataa tgattattat atagagaatg attttaataa accaagaaaa tatgacaacc
     2701 tatacaattt tatacacaat agaacaacaa taaataacaa cagtaacact cgagttttga
     2761 ttaattcagc atattttcat attaaatatt taaagaaata tgagtatgaa gaagttgtat
     2821 tagattatgt gctaaataga cagtcatttt taatttatgc aataaataac ggaaatttgc
     2881 caagcaataa aaagaacata attattgaaa catatgccta tctggagggg ggatctaaaa
     2941 aactaattaa gtcatgtaat atagagtatc aattatcagt aggagaaatt aaatgtgttt
     3001 tcgaagaaga tttagaactt gacttaaaaa aaatttttgc cagcattcat gattatatag
     3061 gttt
//
LOCUS       DAFEZB010000146         3003 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFEZB010000146
VERSION     DAFEZB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.158"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            28..1170
                     /gene="dcm"
                     /locus_tag="LP859_002804"
     CDS             28..1170
                     /gene="dcm"
                     /locus_tag="LP859_002804"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBL3030683.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AALLRPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYIPQSRERI
                     FLIGHSRDACTEQVFPIIGPSSTSDQNIRNLVNINPSNRGMGGQVYGSDGVAPTLTGD
                     EGIKIALPARDGISVAGLLPGKFEQGNRVYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVQKCDL"
     gene            1160..1450
                     /locus_tag="LP859_002805"
     CDS             1160..1450
                     /locus_tag="LP859_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030684.1"
                     /translation="MIYDELVGEIYWVIEKIQSDPELEEQLRRLNFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            1708..2892
                     /locus_tag="LP859_002806"
     CDS             1708..2892
                     /locus_tag="LP859_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3030685.1"
                     /translation="MVQQNLDYRLLKDRRNQYGVSQNKLATACGLSRPYLNQIENGGV
                     TTSTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVQKIVQEILHLKFDYMLHED
                     YAFYSYQEQYIMGDIVVMLSHEEEKGVLLELKGRGCRQFETFLLAQKRSWYDFFDDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDKSETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIEGTTFSIINRYLRFTDKVEGKRRTNWPLNERWGRFIGRNRKEIQLTSDPKPYT
                     IERTLSWLGRQVAPTWKMAKELDRLNQTTYILDMVQNAQLSDRHKKILEQQSMAIENL
                     II"
     gene            2889..2987
                     /locus_tag="LP859_002807"
     CDS             2889..2987
                     /locus_tag="LP859_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL3030686.1"
                     /translation="MKRGSSMLGITVMCLIHTSAAADRVMERKVIK"
BASE COUNT          928 a          528 c          718 g          829 t
ORIGIN      
        1 ttttctttgc aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt
       61 ggctttcgtt taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat
      121 aaatttgcta gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat
      181 gacatcacaa gtgtatcaga tgagtttatt caatccctcg gaccagttga catcctttgc
      241 ggcggatttc cgtgtcaagc tttttcaatt gcgggaaagc gacaaggatt ttccgatact
      301 cgaggtactt tattctttga aatcgctcgg ttcgccgctc ttctccgacc taagttttta
      361 ttccttgaga acgtccgggg attgctcaat cacgaagggg gggctacgtt cgagacgatc
      421 ctccgaacgc tggatggatt ggggtatgac gtggagtggc aggtgcttaa ctcaaaggcc
      481 tacattcccc aaagccggga gcgtatcttc cttatcggac attctcgaga cgcatgtacc
      541 gaacaagtat ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg
      601 gtaaatatca atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta
      661 gcgccaacct taacaggtga tgaaggaatc aaaattgcac tgccagcaag ggatggaatc
      721 tctgtagcag gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact
      781 ggaaccgccc ccaccttgtc cacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat
      841 gaaacagaat atcaagaggt gaagccagga gacagtgtga acctggcttt tcctaattcc
      901 acaagtcgcc gaggtcgtct gggaaaacag agtgttcata ctttactgac aggagaccaa
      961 caagcgattg tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga
     1021 cttcagggct ttcccgattg ggcttttgat cgagcttctc aagtcaattc agatagtcag
     1081 ttgtataagc aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga
     1141 ttaaaggagg tacaaaagtg tgatttatga tgagttggtt ggagaaattt attgggtgat
     1201 tgaaaagatt cagtcagacc cagaattaga agaacaattg cgacggttaa actttgatat
     1261 tcgcaagaat ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc
     1321 tcgaattgaa ataaaccaag taattgcaga atttgagcgg attgcggacc aagcaaaaga
     1381 accggatatt cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac
     1441 agatgagtaa cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga
     1501 agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg cgttagccgg ccatgatgtc
     1561 tcaccccccg tatctaacag gggggtacaa aaacacaaaa aacaagcaag cagcgattaa
     1621 gaatggttgt catgacgagg atagttagct gtttgaacag gtgttaactc attttgaaaa
     1681 gttaacacct tatttggagg tgaaaacttg gtacaacaga acttagatta tcgtttacta
     1741 aaagatcggc gcaatcaata tggtgtttct caaaataaat tggctactgc ttgtggattg
     1801 agtcgtccct atttgaatca aatcgaaaat ggtggcgtaa ctacatctac aaaaactatg
     1861 agaaaaattt ttgaccaact ggaaagtttc aatcccgatt tgcctttgac gttactgttt
     1921 gattatgtaa ggattcgttt tccgacaacg gatgttcaaa aaattgttca agagattttg
     1981 catttgaaat ttgactatat gcttcatgaa gactatgcct tttattcgta ccaagaacaa
     2041 tacatcatgg gtgatattgt ggtgatgctt tctcatgagg aagagaaagg cgttctttta
     2101 gaattaaaag gtcgtggttg tcggcagttt gaaacttttt tgctcgctca aaagcgtagc
     2161 tggtacgact ttttcgatga ttgcttaaaa gctggaggcg tcatgaaacg cttggatttg
     2221 gcaatcaatg atcgagttgg cttattggat attcctgagt tgacgaaaaa atgtcagaag
     2281 gaagaatgta tctccttatt ccggactttt aagagttacc gctcgggtga acttttgaaa
     2341 gctgatgaaa aggatggaat gggaaatact ttgtatatcg gaagccttaa gagtgaggtt
     2401 tatttttgtt tatacgaaaa agactatgaa caatatatca aattgggaat tccgttagac
     2461 aagagcgaaa cgaagaatcg ctttgaaatt cgattgaaaa atgaccgtgc ctaccatgca
     2521 atacaagact tgttaaaagg tcgaagtatt gagggtacca cattttccat tatcaaccgt
     2581 tacttacggt tcacggataa agttgaaggg aaacggcgaa caaactggcc gctaaatgaa
     2641 cgatggggtc gctttatcgg gcgaaatcgc aaggaaattc aactgacttc tgaccccaaa
     2701 ccttatacta tcgagcgaac actgagctgg ctaggtcgac aggtagcacc tacgtggaag
     2761 atggctaagg agttagatcg gttaaatcag acgacttata ttcttgatat ggtacagaat
     2821 gctcagctat cggatcggca taaaaagatt ttggaacaac aaagtatggc aatagaaaac
     2881 ttgattatat gaaaagagga agcagtatgc ttggtattac cgtgatgtgc ttaattcata
     2941 caagtgcggc tgcagatcga gtaatggaaa ggaaagtgat aaaatgaatt tcggacaaaa
     3001 ttt
//
LOCUS       DAFEZB010000147         2806 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFEZB010000147
VERSION     DAFEZB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.159"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(75..425)
                     /locus_tag="LP859_002808"
     CDS             complement(75..425)
                     /locus_tag="LP859_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030687.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(418..1743)
                     /locus_tag="LP859_002809"
     CDS             complement(418..1743)
                     /locus_tag="LP859_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL3030688.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2193..>2806
                     /locus_tag="LP859_002810"
     CDS             2193..>2806
                     /locus_tag="LP859_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3030689.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT          848 a          562 c          458 g          938 t
ORIGIN      
        1 ttggccataa ccccaaatct cttctgatag ctgatcaaag gttatatact ttagctcata
       61 ttgctggata atgttcattc taatcgactc catttattat cctggtaaaa ttcgatatga
      121 cgtatttcgt cgtagtgaat tttgtctttt ccaacgtaga tccccagctc atcactacct
      181 aagatcttgc caacaatatc ttctggatag tatccttcag cgtttcgttc ttctttttga
      241 atagatactg atctattttt tatgactgca gcgttcaata cttccgcaat ctcttcagtc
      301 gtcatttgtt cttttgctaa attttgactg gttcgttgtt cattgtcggc atcaatttct
      361 tcagtatgat cagataaata aaaccctgcc cactttaaac gtccacgatc attgtaatca
      421 atcattttta attcccccat ttccgcccgc atgaccacca acaagtgtgc ttctagtaat
      481 gcttctagcg ctttctaaca ttgaagaagc atgtacaatc gacgtaaaac catatttttg
      541 ccgaatcttg tcaataattt tatctaaatt ttcttcgtta atttgctttt gtggatcact
      601 aaataaatcc agctgaagcg aatcggtgta cacaagcttg ctataggtta ttccaacgtg
      661 ccttacttct tgtccttcat aatacttatc aaacaagaaa agacaataat tctgcaattc
      721 ctttgtatta tctgtgatag gtattttcat ctggtgtgaa aatcctggtc tcgtttctaa
      781 gatcgacgta ccaatgttta aatgaacaca tcctgtttta caattatgct gcctgatacg
      841 aatggctact tgttccgcca tttcttttac caccagctcg atttcatttc ttctagtata
      901 atctcgtggt aacacttgtg aattaccata agatttttcc tttgtcggtt ctggcgggat
      961 tgctatatct gttcgatcga ttccgttagc atggaagtac agctgtaagc caatcacacc
     1021 tagtctattc ttaatcgtat aaggattcca gttagcaaga tctctaatgc tcataatccc
     1081 catctgattg agtctttttt tcattcgaga gccaattccc caaaagtccg tcatttcagg
     1141 aatattccat actttttcag gaacgttttc gtacgtccat tccgcaacaa atccttcatt
     1201 atgctttgct tcgttgtcta aggctagttt agccaaaagg ggattatctc cgacacctac
     1261 tgtagcaatc aatcctagct cttctttaat attctcctgg atcatttgag ccagcttctt
     1321 tctccgttga cttcgtgttc cttcagtcgt aaaaagattc agtgatcggg tcacttttaa
     1381 gattgattca tcgatcgagt aaatcagtag atcttcatca gccacatatc ttctgaaaat
     1441 attatttacc tgcatatttc gcttgatata taggttcata cgtggtggaa caacgtgtag
     1501 tgttttagga aatggttgtg gtaagtcacg tggtctactc acatttgtaa taccataccg
     1561 cttttttgct tcaggagaag aagctaatat caatcctgaa ccagtattat cagctcgact
     1621 cataacaaca agttctgttg ttaatggatc taaatttctt tctatacatt cgacacttgc
     1681 gtaaaagctt ttcatatcga ttaaaaagta atcatttact ggctcttttg aataatccat
     1741 cataaataac actccattct ttcacattat acaaacatat gttcgatatt gtaaagaaga
     1801 aatagctaaa tacataaaaa aagaagcata ggtatgctat caccctgctt cttcttttga
     1861 tttgtatgat caatttattc tatcacttta ctaattaatt tgaaaatcta ataaaaactc
     1921 tcactctaaa agagtgagag actgcaaatt agagtacgga cttttttgga gttccgttct
     1981 ttaatattaa caaatgaata aaatccttac aacatttgag aataagttat cagtagacac
     2041 ttacaaaaaa ttctcatact atttcctcca tcattagttt ttgtctatat aaggtatacc
     2101 ctaactagac acgaccgcga cattgaaaaa tctggttttc tcgtgtatga ttagggtgtt
     2161 ttttaaatag ttgcagaaag taggtaaaaa ttttgagagt tgcatacata cgtgtctcat
     2221 ccatcgatca aaatgaacaa cggcagattg aagaaatgaa aaaattcggt gcagagagaa
     2281 ttttcattga gaaacaatct ggcgcaacga ttactcatcg acctgttttt caagaagcac
     2341 tggatttcgt ccgagatcaa gatattttta ttgtggaagc tattgatcga cttggaagaa
     2401 attacgacga gatcatcgct tcagtcaatt acttgaagaa gaaaaatgtg cagctgatca
     2461 tcacaagtct accgatcatg gccgaagcaa ttggcaatcc tctcctggat aaattcatta
     2521 aagatttgat cattcaaatt ttggccatga tcgctgaaca agaacgtacc gaatctaaac
     2581 gacgtcaggc acaaggcatt aagattgcaa aagcgaacgg tgtttataaa ggacgtccta
     2641 agctatacag tgccaatgca aaagatcccc aacgtcgtct tgtttataaa aacatcgttg
     2701 aggatctaaa aaaaggtgtt gcgatcgcta agattgcaaa agactacaat gttacgagac
     2761 aaaccgtata tagaataaaa aaagacagca tggtcaatca cgaata
//
LOCUS       DAFEZB010000148         2786 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFEZB010000148
VERSION     DAFEZB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.160"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            783..2660
                     /gene="ltrA"
                     /locus_tag="LP859_002811"
     CDS             783..2660
                     /gene="ltrA"
                     /locus_tag="LP859_002811"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3030690.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
BASE COUNT         1061 a          436 c          615 g          674 t
ORIGIN      
        1 aacaagactt caaacgcttc ttggcgatga ttgatgagtg ccataatggt gcgacctgtt
       61 ctgcagtgaa aagctgtggg ttgcaagtag tctaaaaact attgtaataa ctcttataac
      121 gagaagtcaa atgagtgggt aacgccacaa agggcttcca ctgatactcc gacatgcaac
      181 aagtagttta tgcagaccaa cgttgtgtga agctcggtga agtcggctga aggataccgt
      241 aaggtaaaag aaatagtgaa taccacgaaa gttctttaga ggtaaagggt gtatccgata
      301 agtcgggtgt ccaaaaaaac ttaatatggt gagtatgtac aaacgtactg acgaactagc
      361 gaatgtactg tccgaaaggc atagctccta gaaatgggag agacagtgag caaaaaatca
      421 ctcattgtgc taacttgtgg ttgagtaaaa gtcgttgtta tgaaagacca tgctgcgtta
      481 caggcgtagg agaagttagg gggcaaaagc tagcatctaa gttaaggcat gtggatagat
      541 ataagggaac aggggaagcc atcaacatgg agagtgcata ctcgatgaag tggtagtaag
      601 aaatatgtga atgacaaata acttacttta gtgatggtga aagtagagtc acgactgatg
      661 aaggttctgt aatggaactg gaggaacagg ctcgattcgt ctgtaaaagt catgaaaccc
      721 gttcaaccat gggattctcg tatgactaaa aaggtcacga gccaattgga gcgtgataaa
      781 ccatgacaaa agaaacaagt ccaaaagata ggaagttgcg gcatgcagaa tattatggca
      841 tgacggaaat cttcgataag atatactcca aaagtttgaa gggagataat ttcaagaatc
      901 taatgcagat tatcatttct gataacaaca ttgtcctagc atatcgtaat atcaagagaa
      961 atcacggtag tgtgacaaga ggagaagatg gagtaacgat taaagatatt gaaaatctca
     1021 accgtaatga attcgtagaa attgttagga agcgattcgc acattataac cctcggaaag
     1081 taaggcgtgt agaaattcct aaatataatg gtaaattaag accgttgggt attccatcaa
     1141 tatgggatag aatcgcgcag caatgcatct tacaagtatt agaaccaata tgtgaagcaa
     1201 aattcaataa tcacagctat ggtttccgcc caaatagatc agcagaacac gcaatagcag
     1261 attgtatggc ccgaatgaat caaagtcaca tggaatatgt ggtagatgta gatatcaaag
     1321 gtttcttcga cgaagtcaat catactaaac tcatgagaca aatctggtct atgggtatac
     1381 gagacaaaca gcttctctta atcattagga aaatgttaag agcacccgta gtacttccaa
     1441 atgggaagtt gatataccca acaaaaggaa caccacaagg cggaatactt agcccattac
     1501 ttgcgaacat caatctcaac gaatttgact ggtggatagc aaatcagtgg gaggaaaaag
     1561 atgccaaaga gctaaaacat cattttagga aaaatggagc ttgggataaa ggtaatatct
     1621 atcacaagct cagaaagagt acaactctaa aagaaatata cctagtgaga tacgcagatg
     1681 attttaaaat tttctgtaga aattataatg aagcgaaaaa agttttctat gcaactaaaa
     1741 tgtggttaca ggagcgtcta aagttaccaa tatctgaaga aaagtcaaaa atcacaaatc
     1801 tttcaaaaga agaaagtgaa tttttaggct ttacgatgaa attagtgacc aaaggtaaca
     1861 aaaaggttgt taatacgcat ataggtacaa aagcacaaag catgattaaa gtgaaactaa
     1921 aaaaacaaat caaacgaatt caaaaaagtc ctaatagcaa agataccttt atctatattg
     1981 gagtctataa cagtatggtt attgggattc ataattactt caaaattgcg actcattgta
     2041 gtgtagcaat gcgaaaaatt gcaagagaac tcagaccaag ttttgaaata cgactacgag
     2101 aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga aggatacaag ccttacctac
     2161 ggtcaggaca aatacgatat ataagagggt ttccaatatt accagtaggg tatattcaac
     2221 acagacatcc gatgtgtaaa aaaagaaata ttaataaata caccgaagaa ggtagggaag
     2281 ccattcataa gaagcaacaa accgtagaag aatggaagat atcgtggttg tgtagcaatc
     2341 cagtgcttag taagagagca acgattgaat ttgcggacaa tcgaatttca aaattcatcg
     2401 ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat cttatctgaa atgcattgtc
     2461 atcatattat cccttatcat gaaagcaaaa gtgatagcta tgaaaaccta gtgattgtta
     2521 ctgaggaggt tcatcgggta atacacgcta cacaaagtga aacgattgag gaacttctaa
     2581 aatatctaaa attaaatcca aaacaaaaag aaaaactcaa tgagttgaga ttaaaagttg
     2641 gtaacgaaga aatatcttaa taaatatttg taggacggga aaacagaatg tcttttatag
     2701 acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt gtggactggg ggaaaagacg
     2761 gagataatat caaactctta cctatc
//
LOCUS       DAFEZB010000149         2719 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFEZB010000149
VERSION     DAFEZB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.161"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="LP859_002812"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="LP859_002812"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3030691.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DAFEZB010000150         2622 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFEZB010000150
VERSION     DAFEZB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.162"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="LP859_002813"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="LP859_002813"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3030692.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          940 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg ga
//
LOCUS       DAFEZB010000151         2509 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFEZB010000151
VERSION     DAFEZB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.166"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            349..1929
                     /locus_tag="LP859_002814"
     CDS             349..1929
                     /locus_tag="LP859_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL3030693.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2127..2351
                     /locus_tag="LP859_002815"
     CDS             2127..2351
                     /locus_tag="LP859_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL3030694.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          892 a          404 c          530 g          683 t
ORIGIN      
        1 ttttctttta gtcagctgac aaaaaaatat ttgactctta tggtttggtg gctttttcat
       61 tcgctgaatc catgctatac tgattcagtt gtagtagttg acaaaggttg tgaaaaagct
      121 atcctgcatt aagtaataag agcagccaag caaaaacagc ttttcgtatt tttggcttgg
      181 gtgggcttat tgctgatagg ctgtctctag aacgccgttt actcggaaaa agagactgct
      241 acacggcttt tgtcacagcc ctatatgaaa ttgtctttct ttaagagatc ggacaatctg
      301 tgcctagaga aaatataaat aattgaatgg aagaaatgga gttaggttat gaataatcca
      361 aaattaaagc aacaagtgga caatcgcaga acttttgcga tcatttccca tccggatgct
      421 gggaaaacaa cgattacaga acaattacta ttgtttggtg gggctatccg tcaagccggg
      481 accgtaaaag gcaaaaaaac aggtaatttt gccaaatcag actggatgga aatcgagaaa
      541 caacgtggga tttctgttac tagttctgtt atgcagtttg attatcaagg gaaacgagta
      601 aatatcttgg atacaccagg acatgaagat ttttctgaag atacttatcg tactttgatg
      661 gccgtggata gtgcggtcat ggtaatcgat agtgcaaaag ggatcgaagc acagacaaag
      721 aaattattcc aagttgtaaa aaaacgtggc atcccgatat ttacttttat caacaaacta
      781 gatcgtgatg gtcgtgaacc attggaattg ttagaagaat tagaagagct attagatatc
      841 gaatcttatc cgatgaattg gccaatcggt atgggtaaag ggctagaagg tctttatgat
      901 atctaccata atcgtgtaga gttttatcgt ccggaaaatt atcaggatga acgattagta
      961 gaattggatg aagatggact gcttcccgaa aatcatcctc taactgaaaa ttccttatat
     1021 gaacaagtct tagaagaagt agaactaatc aaagaggctg gagatgcatt taatcaagaa
     1081 aaaattgctc gtggcgatca gacaccagta ttttttggtt ctgcattgac taacttcggt
     1141 gtacagactt ttttagagac tttcgttgaa ttggcaccag ctccttatgg acacaaaaca
     1201 caagatgatc agatggtaag tccttatgag gaagaatttt caggtttcgt attcaagatc
     1261 caagcaaata tgaatccagc ccatcgtgac cgaatcgctt ttgtccgaat ctgttctgga
     1321 acattcgaac gaggaatgga tgtctggttg gaacgtacaa ataaaaaact gaaattaagc
     1381 aatgtcacac aatttatggc agattcgaga gaaaacgtcg aaaaagcagt agcaggcgat
     1441 atcatcggtg tatatgatac aggcaattat cagattgggg atacattgtt cgaaggaaaa
     1501 ctaaaagttg cttatgaaga actcccttca tttactccag agctgtttat gaaagtgact
     1561 gcaaaaaatg tcatgaaaca aaaatccttc cacaaaggaa tctatcaatt agttcaagaa
     1621 ggagcgattc agctttataa aacatatcta acggaagaat atatcattgg agctgttggt
     1681 cagctacaat tcgaagtatt ccaatatcgt atgttaaatg agtataacgc cgaagtaatc
     1741 atgtctccaa tgggacacaa aatcgctcgc tggatcgatc cagaagattt ggatgagaag
     1801 atgagttcaa gtcgcaatat cttggctcgt gaccgtttcg accaaccgtt gttcttattc
     1861 gagaaccaat ttgcagaacg ctggtttgcg gataaatatc ccaatgtaaa attaaaaagt
     1921 ttgatgtaaa gaagtcttga cacaagctgc cctgcatcaa gtaataagaa tagccaaaca
     1981 aaaacagcct ctcatatttt gtttagagcc gtgacggatt ttccgtgacg gctcttttta
     2041 ctatattaaa atcatagaaa tgtctaatca aaaaaatata aaaattattt tgcgtacata
     2101 tgattaaaga aaggagagtg tgacacatgg aaacagaatg gcaaagacgt gaaagaaaaa
     2161 aacagaaaaa agaagagatg ttacgattat gtcgattatt agatgtaaaa gactgggaca
     2221 aaaacgagca aattgcacaa aaagtacgat caatcatcca taaagaggaa aaagttaaaa
     2281 ataaagacac gcgtagaact gaaaatgaaa agagaaaagt acaaaagaaa agagaaaaat
     2341 caaaaagcta gaaagaaaaa gaggctgaga cagaagcgtt taactccaag aaataagctg
     2401 gaattcacaa aaatttgaaa agcaattttc gtgaatttca gcttatttcc gaaggagttg
     2461 cttctgcttt cgccgtttat acgtgtttag agcgtgagac aaaagtgtt
//
LOCUS       DAFEZB010000152         2483 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFEZB010000152
VERSION     DAFEZB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.167"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(1014..1304)
                     /locus_tag="LP859_002816"
     CDS             complement(1014..1304)
                     /locus_tag="LP859_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030695.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="LP859_002817"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="LP859_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DAFEZB010000153         2400 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFEZB010000153
VERSION     DAFEZB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.168"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            207..2069
                     /locus_tag="LP859_002818"
     CDS             207..2069
                     /locus_tag="LP859_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3030696.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="LP859_002819"
     CDS             2069..>2400
                     /locus_tag="LP859_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3030697.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAFEZB010000154         2318 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFEZB010000154
VERSION     DAFEZB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.170"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..141)
                     /locus_tag="LP859_002820"
     CDS             complement(<1..141)
                     /locus_tag="LP859_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HBL3030698.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAK"
     gene            398..868
                     /locus_tag="LP859_002821"
     CDS             398..868
                     /locus_tag="LP859_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL3030699.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            1090..2193
                     /locus_tag="LP859_002822"
     CDS             1090..2193
                     /locus_tag="LP859_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL3030700.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          778 a          375 c          520 g          645 t
ORIGIN      
        1 cttagccagt tcagaaaaac caaagatgtt catcagctct ttcactagga cgagacccga
       61 atcactcgat aattgaccac ctgtatgaga aatagtgata tttgaattga attttacttg
      121 gtttttgtgt aagctagtca tgaaaagaac tcctttcttt tggttggttt agtcacttta
      181 accatagcag aaaggggttc ttttttcatc acttaacggg tgaagatgaa aaagtaattg
      241 taagctgccg tgaggcagtt tcacatggtt ttaattaaaa gtatgaatta ttcaggtaga
      301 atctctgaag aataaatcac gtgatcgtga cgataaaata cacttacgtc aggtgataaa
      361 aaatacataa gtagaaaact aagaggagga acagaaaatg aaagaagttc aattgatcag
      421 aaaaagcgaa ctatcagaag gtggttgcaa tgcatgcggc gtagtagaag caacaagcta
      481 tacattaaag cttggtgcca acaaagcgat aatctctgag ttgacagtag gcggtcttgt
      541 ggacagtctg gcgcttgctg aaggttttat tggagaagat atttatgaga tgttttcaga
      601 aatccgtcag ttaaaaaaag gggaaaactg catagaggtt catcatgaga gcccgaatgt
      661 gagattcaaa cgtggtgata atgagatgat cttcaataat catgtgtccg atcacaccga
      721 gctatatgga atcgtgaatc aaatactaac tgagcttttt ggattgggac cttatgcgtt
      781 caaggaagaa aacggtaatc caaaattaaa cgaagaatgg caagaaacga ttgaaataca
      841 aagaaacaac ccgcacttat ttcaataggc atccacagta tttcgtaaaa gggactttga
      901 cacgactttt gtcaaaatcc catcgttttt tatctatttg tgttctaaac atttctttca
      961 gagtagtaac aactggaatg actgaaatgt aaacagatta tttgcctgta tcaatacgga
     1021 agagtaatat aaagtgtgta aacgctatca tattattcat tcaatttttt atagagggaa
     1081 ggcattgaaa tggaaaaaac gtatcaagca ggtacaaatg aaggaatagt ggattttatc
     1141 aatatggaag atttagaaat agccgcttca caagtgattc cagctggtgg ttatggttac
     1201 atcagcagtg gtgctggcga cttatttact tatcaagaaa atgaacgggc attcaatcat
     1261 cagctgatta ttcctcatgt attaagagat gtagaattac ccgatacaac tacacatttt
     1321 gacaaagaaa cgctgactgc accaatcatc atggcaccag ttgctgctca tggcttagct
     1381 catgtgaagg cggagaaagc ttctgcaaaa ggtgtagcag attttgggac gatctataca
     1441 gcaagttctt acgcttcttg tacattagaa gagataagag aagcaggcgg ggagaaagca
     1501 ccgcaatggt ttcaatttta tatgagcaaa gatgatggga tcaatttgga tattcttgaa
     1561 gtggccaagc gtaatggagc aaaagcaatc gtattgacag cggatgcaac agttggcgga
     1621 aaccgtgaaa cagaccgcag aaatgggttt accttcccac tgccaatgcc gatcgttcag
     1681 gcttatcaat caggagtagg ccaaacgatg gatgctgtgt acaaatcttc caaacagaag
     1741 cttagtccaa aagatgtaga atttattgct gctcattctg atttgccagt ctatgtgaaa
     1801 ggtgtccagt cagaagaaga tgtctatcgt tcattagaat ctggtgctgg cggaatctgg
     1861 gtatctaatc atggtggccg tcaactagat ggtggtccag cagcgtttga ttcgttgcaa
     1921 tatgtggcag aagcagtaga taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgc
     1981 ggacagcatg ttttcaaagc aatcgcatca ggtgcagatc ttgttgctat tggcagaccc
     2041 gttatttatg gattgtcttt aggaggaagt acaggagtcc atcaagtgtt tgatttcttt
     2101 aaaacagaat tggaaatggt catgcaatta gctggaacac aaacagtaga agatatcaaa
     2161 aaaataaaat tgcgtgagaa tcgttttatt taagaaacaa atgacgaaaa aaataggagt
     2221 gcggcagatg atgccgtact cctattttta tagttcaagg atgtcactaa tagaataatt
     2281 tgaaacgaaa aaataaaatt gaaatatttt ttcatact
//
LOCUS       DAFEZB010000155         2230 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFEZB010000155
VERSION     DAFEZB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.173"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..376
                     /locus_tag="LP859_002823"
     CDS             <1..376
                     /locus_tag="LP859_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL3030701.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="LP859_002824"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="LP859_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL3030702.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..2024
                     /locus_tag="LP859_002825"
     CDS             1857..2024
                     /locus_tag="LP859_002825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030703.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2078..>2230
                     /locus_tag="LP859_002826"
     CDS             2078..>2230
                     /locus_tag="LP859_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3030704.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          626 a          371 c          483 g          750 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt actggttctg ttgcaaagtt ttaaatctac tatcaaataa
     2041 ggtagaataa tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca
     2101 atttcagcag gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta
     2161 tcgtgaagtt cgagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta
     2221 tcgttgggtg
//
LOCUS       DAFEZB010000156         2194 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFEZB010000156
VERSION     DAFEZB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.174"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     misc_feature    109..234
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            278..802
                     /gene="infC"
                     /locus_tag="LP859_002827"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="LP859_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL3030705.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="LP859_002828"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="LP859_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL3030706.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="LP859_002829"
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="LP859_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL3030707.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1838..>2194
                     /locus_tag="LP859_002830"
     CDS             1838..>2194
                     /locus_tag="LP859_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL3030708.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          735 a          384 c          479 g          596 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggt
//
LOCUS       DAFEZB010000157         2189 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFEZB010000157
VERSION     DAFEZB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.175"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(325..528)
                     /locus_tag="LP859_002831"
     CDS             complement(325..528)
                     /locus_tag="LP859_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030709.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="LP859_002832"
     CDS             complement(987..1952)
                     /locus_tag="LP859_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL3030710.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAFEZB010000158         2151 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFEZB010000158
VERSION     DAFEZB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.176"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            526..>2151
                     /locus_tag="LP859_002833"
     CDS             526..>2151
                     /locus_tag="LP859_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030711.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIED"
BASE COUNT          857 a          345 c          483 g          466 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaattgag
       61 aagatgtttg aacaccagag aaagaccaac ggtcagccaa tgaacgataa gagaaatggg
      121 cattcttggt ttaggcaaca agaacgttta gaaaacaaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggctataaca agtacggcgg cctggatatg acaatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac
      361 caacggaaat tggaaacatt agaacagctg aaagaaagat cagaaaaagg aaaagaaaat
      421 cttcttcctg aagtacaagc aattattgac agcggacgag tgacgcaatg gaaaaaaaat
      481 ccaaccattt attttttgaa agactatcga aaagttgctt tagaactgaa tgaaaacggg
      541 ggatttgagg aatcaaaaaa ataccaagcc acgtcggaag aggaaaaagc agctgttcgt
      601 gaattacttg aagaggctgc gccaactgat tctttgaagg agaaagaaaa cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
      721 atcgtaacat tagaattgat cgatcaactc aaaacgttgg ataaccaata cgccgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccca tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaagcaaga agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcataccc attctcctga agacgtgaaa gaacttttga attttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
     1201 ttaggtcgtg ctgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaacagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttaaaacct
     1441 gaagactatc cgaaaatctt tccgaaccgt gtctttgatt ttcaattgga taaaaaagga
     1501 cagtcagaac ttcaagctgg gattcaagcg gtagccaaca atattggaat acaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcta caagtattca tgaattagcc catgcaagaa tgcacaaatt ttcagaatta
     1741 gataccgcaa ctaaagaatt gcaggcagaa atgacttctt atattgtgtg caaacatttt
     1801 ggaatggaca cttcggagaa agctattcct tatattgcag catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccaac cagcagaagt gaagcaggag cataaaaata ctgtagaaac ggcagagaat
     2041 aagaatattg tgcccgttgg ttcactttgg atcgacaaga aagacgggaa agtaatggaa
     2101 gaagatacgg gtcgaacgtt acatttgaag gatgcggcgt ttattgaaga t
//
LOCUS       DAFEZB010000159         2151 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFEZB010000159
VERSION     DAFEZB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.177"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            1..600
                     /locus_tag="LP859_002834"
                     /pseudo
     CDS             1..600
                     /locus_tag="LP859_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            634..>2151
                     /locus_tag="LP859_002835"
     CDS             634..>2151
                     /locus_tag="LP859_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030712.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVK
                     GFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIF
                     PNRVFDFQLDQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFT
                     GEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMD
                     TSEKAIPYIATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPE
                     IQPAEVKQDEKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIED"
BASE COUNT          853 a          360 c          478 g          460 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaatga tcaaatcgaa
       61 aagatgtttg aacaccaaag aaagaccaac ggtcaaccaa tgaacgataa gagaaacggg
      121 cattcttggt ttaggcaaca agaacgaatc gaaaataaag tccaaagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggttataaca agtacggtgg cctggatatg acgatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ctgccacgat ccgaaaatac
      361 caacgaaaat tggaaacatt agaacagctg aaagaaaaat cagagaaagg aaaagaaaat
      421 attcttcctg aagtacaagc gatcattgac agcggacgag tgacgcaatg gaaaaagaat
      481 ccaactatct attttctgaa aggctatcga aaagtcgctt tagaactcaa tgaaaacggg
      541 ggatttgagg aatctaaaaa ataccaagcc acgtcggaag aagaaaaggc agctgttcgt
      601 gaattacttg aagaagctac acctactgat tctgtgaagg agaaagaaac cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gcaaaagggt tgaaaagtta tggtggggag
      721 atcgtaacac tagaattgat cgatcaactc aaaacactgg ataaccaata cgccgcagaa
      781 gcaggctatt ataaattcta ttttgaagaa gtgaagaatg gagaagtagt agatcagata
      841 cgaatggatc taggggatgg cgtcaatagt aatcacccca tctatttaga gttagctaga
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaaacag tcaaaaatga acctatttca
      961 aaagtacaaa atgagattaa agagacaaaa acaaaacaaa agggttatca aaaacgaacg
     1021 gcagaagaga ttaagcaaga agtcaaatca ctttcccaac aggcgcttga tgcggtgaaa
     1081 aagcatactc attcacctga agacgtcaaa gaacttttga attttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatc gaggaacaat ttccaggcgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaacagagg ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca attgaagcct
     1441 gaagactatc cgaaaatctt cccgaaccgc gtctttgatt ttcaattgga tcaaacaggg
     1501 catacagaac ttcaagttgg gattcaagcg gtagccaaaa atattggaat tcaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcca caagtattca cgaattagcc catgctagaa tgcacaaatt ttcagaatta
     1741 gatactgcaa caaaagaact acaggcagaa atgacctcct atattgtgtg caagcatttt
     1801 gggatggaca cctcggagaa agctattcct tatattgcga catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc tgcggaacgt gggaaaatca tgaatgatgt cagtcgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccagc cagcagaagt gaagcaggat gagaaaaatg ttgtagaaac agcagaacaa
     2041 aagaatattg tgctcgttgg ctcactttgg atcgacaaga aagacgggaa agtcctggaa
     2101 gaagacacgg gccgaacgtt acatttgaag gatgcggcgt ttattgaaga t
//
LOCUS       DAFEZB010000160         1948 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFEZB010000160
VERSION     DAFEZB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.178"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            181..483
                     /locus_tag="LP859_002836"
     CDS             181..483
                     /locus_tag="LP859_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030713.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(999..1072)
                     /locus_tag="LP859_002837"
     tRNA            complement(999..1072)
                     /locus_tag="LP859_002837"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1627..1941)
                     /locus_tag="LP859_002838"
     CDS             complement(1627..1941)
                     /locus_tag="LP859_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030714.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          289 c          301 g          650 t
ORIGIN      
        1 ttttataagg gtttgagcat tttttatttt cgcagatttt cgttaaatga atgaaaatgg
       61 cattcaaaat agcattcaaa aagaacactt tttaatatat atgttccatc catattatca
      121 atgatattcc tactttacaa aaagaacatt agttcgtata ctctttttga ggtgaacatt
      181 atgttgatgg aaggaaaaac acaattatgg tttaaatttg atccttcgaa tagatttgtc
      241 aaagattttt ataaggtgtg ggattcagaa gttttctttt tagcaatcga agatagctta
      301 ttaatcaatc tctattactc taataagaac tactttaaaa ttccggcaac aaaaaccaga
      361 atgaagaaag atgtgtattt tttgtttgat atcgtgactg atgtgccaga cgcacggagc
      421 gatcatcggc gttatgacta tttgaagtat actttcgttg atccagaaag gtataaagat
      481 taaagtaggc tacctaaaaa aggtagcccg gaacggattt tatcaccata cttataaaag
      541 ggatattttt taagttagta ttaagattgt gtaatatgat gatatctata ttttatagta
      601 tcagtgctat aaaatcaaaa ataagtcact aattaactac cactccaatt ataagtcttt
      661 ttcccattat tttttacagt tatatggtat gctttttaat ggcttcaaat ataaaagagt
      721 ttaaagcgta acacacttat ggggaagtgg tttttgggga acgctttaaa ctcttcttta
      781 ttattatctc acaatataac ccaaatgtct ttccatttaa aaatcaaagt aaaacttttc
      841 aaatatacag aagtataact atgtgaaaca tcctttcatt aatccataaa aggatacata
      901 aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa
      961 aaaattctaa cacagaacga ttcaattggc tacattaatg taccctgtag gactcgaacc
     1021 tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt agtggttgtt
     1081 gccacataaa gccataaaca atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca
     1141 tatcttggag tgtgactatt tatgagtgat agtgaagata tgcgtctcca tatatgtatg
     1201 gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc
     1261 aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca
     1321 ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa
     1381 agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca
     1441 caattatggt ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat
     1501 taattcatcc taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca
     1561 gcaggcttca cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata
     1621 attgctttaa ttattttttg acttctcttc aaacacaaga agtagttgtg taataattgc
     1681 agaaataagg attccaataa tagatataaa atttgacatg gaaacgtcta taacattaaa
     1741 agaataaagt gttgaagaaa gtaaactgat tgaaataacc cctccgtaaa aaatagtctg
     1801 tgaatatacc cacttttttt cagaagacgt acttcgtttg gttctaaaac ctatccctgc
     1861 atttctactc ttatcaaaaa accaaatatt tagaagtaat aatattgcta ttattaagat
     1921 aaaattactg taaaaatcca aaagctat
//
LOCUS       DAFEZB010000161         1930 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFEZB010000161
VERSION     DAFEZB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.179"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(121..1161)
                     /locus_tag="LP859_002839"
     CDS             complement(121..1161)
                     /locus_tag="LP859_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3030715.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          674 a          287 c          231 g          738 t
ORIGIN      
        1 acttataaat tctattaaaa tagctattat tttgtaatat tcgtaatata attagaataa
       61 taaaaaattt ttaattttaa tgttactcca tgaagcaaca atcactttgt cagtaaagtt
      121 tcactctaac cagtctttcg taatagtgct tggggagctt tcataattca aacgtttttc
      181 acttgatact ttagcagcta ttggtttttc gataaaccaa ttttttacat agacaaaaat
      241 caggttctgg atattttcga ctttttcggt ttttaatttt tgataggctt ttaacagtgt
      301 cgtgtataat tcttgttctg cattgacata ataactatcg agttcttcaa aaactatcga
      361 aataccactt tctatttcag cttttttctt tgcattatga atagtctgga cggttttttg
      421 agcttcactg taacttgaag aaaatgtttt tatcaaattt aatactcgtt cagggacaaa
      481 cgtagcaatg ctgtcgtttg tcataattgt ctcaaaattg tctaacaaca attgatcttg
      541 taggcggtct ttttcagtgt ctatattgtg tctattagtg tctaatatat tattattaag
      601 ttctggtatg attttcatac cctctgattt atcaacgttt tggcacggta tgattttcat
      661 accctcttga gccccaaggg attcagcgtc tgatttttcg tcgagggtgg gtatgatttt
      721 cataccctct gatttatcaa cgttttccct attctctcta tcaaattttt cgagtaagta
      781 gatatcattt tctgagactt ttaattctgc tagatatagt ctattaggat tatttttccc
      841 taactgtttg ttaaatccca tctgcttttg taataataaa ccaaaggatt ccaactcttt
      901 tttgattgct atcgcttttt gtttttggca gtttaatact ctacatagtt catttgtcgt
      961 ataagtaaaa taaacattac cttcttcatc gatttgccgt ttgctgattg cgatctctaa
     1021 tcttgctttt aaaagcatat aggcaatctt agttgaatct ttcatttttt tgtattcgtc
     1081 agaaacaata aagacttttg gaaattgata aaataattcg ttgtaaactc tatccgcctt
     1141 aaaaaattta aaatcagtca ttgtagatcc tctactttca cgttgtttta tttatgtaaa
     1201 attacctact cttttcgaaa atagcaaaga taaataaaaa atgcaaacgc attaaaactg
     1261 ttttttaatt aaatactacc taaaatcaga aataaaatga attatgtact acctttaaag
     1321 tcattaagaa ctactttaaa taaatattat ttataattaa atactaccag aaaaacgtat
     1381 gaataaaatt tagttttaaa attaaagata attaagtact accagtgtca aaacaaaaaa
     1441 taaattaaga actaccttat gcatataaaa catactacca ttactagtaa tatatactac
     1501 cttatttcag tagtatatat tatattattc ttataagaca aggctttagg ctagaaaaca
     1561 atagattata tactactaaa aaatacaata agtactacca atgtcagata tatataataa
     1621 ttatgtacta ccattaaaat taacttattt aataagatac tactgtgtta atctgaaaaa
     1681 aattacttta aagtagttac atactacctg ttaatttaga tactaccatt aatacttaaa
     1741 ttagatacta cctatattta tattaagtac taccgttcta agaataagat aataaaaaaa
     1801 tactaccaaa ttgcaattaa atactactta aaatgtaaaa aataagaact acccaaatta
     1861 ggtagttctt aataagagaa tatcagataa atttgtacaa gtcataaaaa aattaatctt
     1921 aaatttttaa
//
LOCUS       DAFEZB010000162         1879 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFEZB010000162
VERSION     DAFEZB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.180"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            316..570
                     /locus_tag="LP859_002840"
     CDS             316..570
                     /locus_tag="LP859_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3030716.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="LP859_002841"
     CDS             571..888
                     /locus_tag="LP859_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL3030717.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..>1879
                     /locus_tag="LP859_002842"
     CDS             1447..>1879
                     /locus_tag="LP859_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030718.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT          663 a          281 c          350 g          585 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatga
//
LOCUS       DAFEZB010000163         1805 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFEZB010000163
VERSION     DAFEZB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.182"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            181..444
                     /locus_tag="LP859_002843"
     CDS             181..444
                     /locus_tag="LP859_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HBL3030719.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            444..779
                     /locus_tag="LP859_002844"
     CDS             444..779
                     /locus_tag="LP859_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3030720.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            853..>1805
                     /locus_tag="LP859_002845"
     CDS             853..>1805
                     /locus_tag="LP859_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL3030721.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLG"
BASE COUNT          588 a          264 c          406 g          547 t
ORIGIN      
        1 tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc tcaaaagaaa
       61 ccattcgtgt tcattttagt ccgaacaaac gtcaattgaa attgtttgat ggtggaaagt
      121 aacttcttgt attttggtgt atacaagtat atactaatgt gtgagagaag gagggattcc
      181 atgttagtaa aagaaatcga aatgaaaaaa tggggaaatt cacaaggtat tcgttttgga
      241 aaagaagaat tgtcagttct aggagaaact aatactgaaa atttaaaatt cgaaatggtg
      301 gtaacagaag gaaaaattgt tttaactcct aaaaataaaa agccagaaac tttagatgaa
      361 ttgttcgcca attatgaagg agaaccctta ggaaaagaag acaaatatga atggggaaat
      421 atgcaaggta gggagatcat ttaatgaaaa agaactatca tgatttatcg caaggagatc
      481 ttgtttatat tgatttaaat cctaccaaag gacatgaaca acggggaaaa cgaccatgta
      541 ttattttatc taaacctaat aaatatttga actatatgat tggtgtcgca ccgataacaa
      601 caaaagcgaa gccttttcct gtacatgttg atttgaatgg tacgcaaaaa ataaaaggac
      661 aaattctttt ggaacaccat cgaatgattg acattgagag tagaggtttt cagtttgtgg
      721 aagctgtgcc aaaggaaatt ttagaagaat gtattgaaaa aattaaatta ttttattaaa
      781 agcagcgaag aataattatt atttcttcgc ttttttgttg tttaaaatta aaaagttagg
      841 agtactgact atatggaaaa tgatacaaat atagttatgg aacatttgaa gcgtcgtgca
      901 aagcgacgct tatttttgtg gttgtttggt acttctgcag gtttgattac tattttgatc
      961 actgtgttcg taaccctttt tctaatactt gcggcgggtt cgattgataa ttcggattcc
     1021 gattcaagta gtggtggcga agcttttaca ggagagtatt cggaaggctt accgatttat
     1081 aaagaaatca aaggaagagg ccctttttct gatgagattg cgcaatatgc cgtaggtgca
     1141 gcagtgaaat ataaattatt accaagtgtg attctttcgc aatacggtta tgaatcagcg
     1201 tttggtacgt cggcgtccgc aagaaatgat ctcaactatt ttgggattac ttggtttgat
     1261 ggttgtctgt ttcctaaagg aactgcacga ggaattggtg gcattgaagg gggctggtac
     1321 atgaagtttc ccaactcaaa agctgcattc tcctattacg gctttatggt agccacacaa
     1381 tcaaatttta atgcatgtgt aggaaataaa agccctggcg caagcttatt gatcttagga
     1441 cgaggtggtt atgccgctgc aggaattact gaagatagtc cgtattatac aggttgtatg
     1501 tcaattatca cttctaataa attaaccgaa tatgatgaat tcgccatcaa acattggggt
     1561 gaaggtggca ataacaatgg cacaattact ggtgaatgga cgaatccatt ccctggcagc
     1621 agtttagata aaagttcctt cagcggtggt caattatttg gaacgaatcc aggcggtgaa
     1681 ttccgtccga acggcttcca tgatggatta gattttggtt cagttgatca tccaggaagt
     1741 gagattcatg cggttcatgg cggaaaagtc gtgtacgttg gcaatcctgg tatttcaggt
     1801 ttggg
//
LOCUS       DAFEZB010000164         1744 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFEZB010000164
VERSION     DAFEZB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.183"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="LP859_002846"
     CDS             131..505
                     /locus_tag="LP859_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL3030722.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LP859_002847"
     CDS             complement(619..1248)
                     /locus_tag="LP859_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3030723.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LP859_002848"
     tRNA            complement(1382..1454)
                     /locus_tag="LP859_002848"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LP859_002849"
     tRNA            1668..1741
                     /locus_tag="LP859_002849"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          602 a          256 c          306 g          580 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaa
//
LOCUS       DAFEZB010000165         1708 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFEZB010000165
VERSION     DAFEZB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.184"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..151)
                     /locus_tag="LP859_002850"
     CDS             complement(<1..151)
                     /locus_tag="LP859_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030724.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESF"
     gene            complement(224..1213)
                     /locus_tag="LP859_002851"
     CDS             complement(224..1213)
                     /locus_tag="LP859_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL3030725.1"
                     /translation="MTDKKQDLINGTFKMKKKNLQKVIDFGGGRFSERTVERMASCGS
                     YLEFIATADKEKKKLIQANFCTNRFCPLCAWRKARKDSLMLSIMMQAIAEEKNYQFIF
                     MTLTTPNVKADKLNEEIDLFNHALSKMFRRKRVMGAIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDPKYYIKQAEWLDLWRDVTGKTGINLDGTDEISQLDIRKVKGFQQ
                     DKAVQELAKYSAKDFEMTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYEAGELDHY
                     KKKDENEYFWFLTASWMKKESSYAINLRELTPDERKFFSGGLVEEKPVEGENE"
BASE COUNT          482 a          398 c          263 g          565 t
ORIGIN      
        1 caaaagattc aaacccaaaa cgcttgcgaa gagccttgcc cattgcagca ttttctttct
       61 caatcaatcg tttcttctgt tggcgaagtt tcgcctgttc tttttttaat ttttcttctt
      121 gcactttaat atctgctaaa gtcatcccca taaactacat ccccttttcc ttggaattaa
      181 taacagaaat ttggtttaat cgttatttta tcaggtgaat cagtcattca ttttccccct
      241 caacaggttt ttcctcaact aacccaccgc taaaaaattt cctctcatca ggcgtcaatt
      301 ctcggagatt tattgcatac gacgattcct ttttcatcca tgaagcagtc aaaaaccaga
      361 aatattcgtt ctcgtctttt ttcttgtagt gatcgagctc gcctgcttcg tatttcttct
      421 tgtactcctt gaagaccccg ttaaacgtga ttaactggcg ccctttcata gcatggtaaa
      481 acgtatcaaa gacttcttgg ctctcggtca tttcaaagtc tttcgcagaa tacttggcaa
      541 gttcctgtac cgctttgtct tgttggaatc ctttgacctt tcgaatatcc aactgactga
      601 tttcgtctgt accatcaaga ttgattcccg ttttcccagt cacatcacgc cacaaatcaa
      661 gccattctgc ctgtttaata taatatttag ggtcagtaaa atacgacttg ttcaccgcca
      721 aaatcaaatg aaaatgcggg ttgtagtcat ttcgtttctt gttgtacgtg atttccaatt
      781 tccggacata gcctttaatc gcacccatga cccttttcct acgaaacatt ttggacaacg
      841 catgattaaa taaatcaatt tcttcgttta gcttgtctgc ttttacattg ggcgttgtta
      901 gcgtcataaa aataaactgg taattctttt cttcagcgat tgcctgcatc ataatcgaca
      961 acatcaacga atctttacgg gctttgcgcc atgcacacag cggacaaaag cgattcgtgc
     1021 aaaaattcgc ctgaatcaat ttctttttct ccttgtcagc ggtggcgata aattccaagt
     1081 atgaaccaca gctagccata cgctccactg tcctttccga aaagcgcccg cccccgaaat
     1141 caatcacctt ttgtaaattt ttctttttca ttttgaatgt cccatttatt aaatcttgtt
     1201 ttttgtctgt catattgata tactgaacct acaattttgt ataaaaaggc tgaaaagccg
     1261 ataacaaaaa aaatagattg ctctccttaa tttggttggt ttttacttag acactaaaag
     1321 cttaatccaa tatgctagga aaagcaatct tttttttgtg caacccctta tgtatcaagg
     1381 tcaaaagcat actatacgca actatttcct gccgaaaata attgcgtatt agtatcaaga
     1441 taagaagaaa catcaaaaaa agaggtcgaa atcgacctct tttttctcgt ttcaaacccc
     1501 aaaactcaaa aacccacttc tgcataaaat tcggtcaatt gaccactgca tttttaaggg
     1561 aagggtcaaa ctttgatact ttaaccgcct gttagaaatg cggttaagtt tttcccactc
     1621 attcttagtc atgcaaaaat aaaaccgctc cctttgaaaa gggcgccccc aaactttcgc
     1681 taacgctagg ttggccaaaa aatggact
//
LOCUS       DAFEZB010000166         1669 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFEZB010000166
VERSION     DAFEZB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.186"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            227..>1669
                     /locus_tag="LP859_002852"
     CDS             227..>1669
                     /locus_tag="LP859_002852"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL3030726.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          567 a          316 c          328 g          458 t
ORIGIN      
        1 taacaaggtt attcgaacct tattttaagt tacttattat ttaatgtatc gaaaaaggtg
       61 aaataggttt gttataataa gaattaagta agacatgaat tgtctaagca aatgtagctt
      121 atccatttca ttaaaataca tatggtgaaa aaacaagttc aaatagtaac taatttttta
      181 aaaaactgat ttataaaaaa tacaacacga atgaggaaaa taaccaatga cagaacaaat
      241 cgaaaaaaat tcaaaaagct tatatcaggc actgtggaat tccgcagata tcctgcgttc
      301 aaaaatggac gctaatgaat acaagagcta tttactaggc ttggtctttt ataaatactt
      361 atccgacaac atgctgcggt atgtctccgt tctattggag gaagaaacgg acgacctaaa
      421 taaagcacaa gacctttacg ttgaagcatt taaagatgca gatataaagg atgatctctt
      481 gtatgaacta aaagatgagt tttcatatac catcgcaccc gcattgacct ttaccgcgca
      541 agtggcagcc attcatgatg gatcttttca attagaagac ctggtacaag gtttccgtga
      601 cattgaacaa tccagcgaaa actttgagaa cttatttgaa gacattgatc tgtattctaa
      661 aaaactgggg tcaaccccac aaaaacaaaa taaaaccatc gcagatgtta tgaaagaatt
      721 agaagggtta aacatggcgg ggcatgctgg cgatgtctta ggcgatgcct atgagtactt
      781 aattggccaa ttcgcatctg aatccggtaa aaaagccggt gaattctata ccccacaacc
      841 ggtcgcaaaa ttgatgacac aaattgtttt acaaggaaaa gaagataaaa aaggcttttc
      901 tgtttacgac gcgaccatgg gttcgggctc gttacttttg aacgctaaaa aatattccca
      961 tcaaccggga accatttctt atttcggaca agagttgaat acgtctacct ataatttggc
     1021 gcgtatgaac atgattttgc atggggttcc cattgccaac caacacttgc ataatgcgga
     1081 taccttggat caagactggc caacagaaga gccaacgaac ttcgatgggg tgctcatgaa
     1141 cccaccgtat tctgcaaaat ggtcggctga taaaggcttc ttagatgacc cacgcttctc
     1201 tgcctatggc gtattagccc ctaaatcaaa agcggacttt gctttcttat tacatggcta
     1261 ctatcatttg aaagatacgg gcgtcatggc cattgttttg ccacatgggg tgctctttag
     1321 agggggcgca gaagggaaga tacgcaaagc cttgttagaa aatggtgcca ttgacaccgt
     1381 cattggctta cctgctaaca tcttctttaa tacgtccatt ccaacgaccg taattatctt
     1441 gaagaaagac cgtgcgaaaa aagatgtgct ctttattgat gcgtcacaag attttgaaaa
     1501 aataaaaacg caaaatacgc tacgagatta ccatattgac gctattttag aggcctataa
     1561 aacccgtaca attaaagaca agtatgctta tgtcgcagaa tttgatgaaa ttgtggagaa
     1621 tgattacaac ttaaacattc cccgttacgt ggataccttt gaagaagaa
//
LOCUS       DAFEZB010000167         1597 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFEZB010000167
VERSION     DAFEZB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.187"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(407..706)
                     /locus_tag="LP859_002853"
     CDS             complement(407..706)
                     /locus_tag="LP859_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030727.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(712..942)
                     /locus_tag="LP859_002854"
     CDS             complement(712..942)
                     /locus_tag="LP859_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030728.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1192..1392)
                     /locus_tag="LP859_002855"
     CDS             complement(1192..1392)
                     /locus_tag="LP859_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3030729.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          557 a          260 c          234 g          546 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagaa agacatttgg gttatattgt gagataataa taaagaagag
      121 tttaaagcgt tccccaaaaa ccactgcccc ataagtgtgt tacgctttaa actcttttat
      181 atttgaagcc attaaaaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttata
      241 attggagtgg tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat
      301 atagatatca tcatattaca caatcttaat actaacttaa aaaatatctc ctttcataag
      361 tatggtgata aaatccgttc cgggctacct ttttaggtag cctactttaa tctttgtatc
      421 tttctggatc aacgaaagta tactttatat agtcataacg ccgatgatcg cttcgagcgt
      481 ctagcacatc agtcacgata tcaaacaaaa aatatacgtc tttcttcatt ctagttttcg
      541 cagcagggat tttaaagtag ttcttattag aatagtagag attaattaat aagctatctt
      601 cgattgctaa aaagaaaact tctgaatccc acaccttata aaaatctttg acaaatctat
      661 tcgaaggatc aaatttaaac cataattgcg tctcattaaa aagcataacc attactccga
      721 tctattttta aacttagtct ctacctctaa tatatatcga gtttttattt tgccttcaga
      781 gaatactgtt tctttttttg cagttacagg ttgtttattt tcggaaaaag ctaatatagc
      841 taaaattgaa acatccatct ggaatttgtc tttttcgttg ctttgctcat aaaaatctgc
      901 atattcatca ctaatatttt ttctaataaa ttcttccatc ataaagatca cctcgaaaag
      961 agtatacgaa caaacgttct ttttgtaaag gtggaattta ttatactaaa taaataggtg
     1021 aacaagtact tgtgccaaat tatgtgccaa aaaaaatcga atttaataaa acacaaacaa
     1081 aaagaaatcc tattatgata acatttctta taataacaaa cacaacagaa gacgtgtaat
     1141 agttagtcag gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt
     1201 ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat
     1261 atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag
     1321 tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta
     1381 gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc
     1441 tatccaacga aagatatatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa
     1501 gccaatggga atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa
     1561 tacttacaac tttccttttc aaagtaccat ataaaaa
//
LOCUS       DAFEZB010000168         1589 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFEZB010000168
VERSION     DAFEZB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.188"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            172..459
                     /locus_tag="LP859_002856"
                     /pseudo
     CDS             172..459
                     /locus_tag="LP859_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="LP859_002857"
     CDS             473..904
                     /locus_tag="LP859_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030730.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="LP859_002858"
     CDS             906..1043
                     /locus_tag="LP859_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL3030731.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1080..1373
                     /locus_tag="LP859_002859"
     CDS             1080..1373
                     /locus_tag="LP859_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030732.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1370..>1589
                     /locus_tag="LP859_002860"
     CDS             1370..>1589
                     /locus_tag="LP859_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL3030733.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          580 a          232 c          339 g          438 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccattatgca ttacagacga agaagcagat aaaatcatta
     1021 ataaagataa gagcgcatct taattgatgc gtttttattt aaggtaaagg agttgtcaca
     1081 tgattaattt aggggaatgg ggaatgatag caggatcaat aaccgctatc gtttctttga
     1141 ttttattagt aattagaccg attgctgcat ctttctcgaa aattactgag actctttcaa
     1201 aagtaagcca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
     1261 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat acagaaaaat
     1321 tggtggaaca cacgcaacaa atcaaaactt tgtttaggga gagaagaaaa tgaataataa
     1381 aacgttcgaa gtactaaaat ggttcgcact ggtaattatt cccgcactag ctactttcgt
     1441 ggggttagtt ggtaaagcgc tcaattggca gtacacagat atctgtgttg tcatcattac
     1501 tggttttggc gcgtttttag ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt
     1561 ctcggctgaa agcgaagaag gaggaaaca
//
LOCUS       DAFEZB010000169         1457 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFEZB010000169
VERSION     DAFEZB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.189"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            109..1041
                     /locus_tag="LP859_002861"
     CDS             109..1041
                     /locus_tag="LP859_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL3030734.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="LP859_002862"
     CDS             1113..>1457
                     /locus_tag="LP859_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030735.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DAFEZB010000170         1434 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFEZB010000170
VERSION     DAFEZB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.190"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            1..366
                     /locus_tag="LP859_002863"
                     /pseudo
     CDS             1..366
                     /locus_tag="LP859_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(601..>1434)
                     /locus_tag="LP859_002864"
     CDS             complement(601..>1434)
                     /locus_tag="LP859_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3030736.1"
                     /translation="VCAIGDEEMTIKERVSFPTTTPEETMPLVIDFFKQYQADLAGIG
                     IGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPISWTTDVNAAAYGEY
                     VFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPF
                     HGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFG
                     GGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLH
                     S"
BASE COUNT          426 a          317 c          275 g          416 t
ORIGIN      
        1 gaagccaaat gaaaaatggt taacagatat tactgaattt agaattcccg cagggaaaat
       61 ctacttatca ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac
      121 aaagacaact tgcttcactc tatgtctaaa aagggttatt cgccagataa ttcagcgtgc
      181 gaaggtttct ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaataataca
      241 acaatcaatc agtttataaa caaattggat aattatattc attggtataa taatcaacga
      301 attaagttat ctttaggagg gctaagccct cttcaatatc ggaagaagca aagatatatt
      361 tgttaaaaag gtccaaaaaa aatatccgca cccctttttt ttgtaaaaaa ctattgcaca
      421 atacaagtag cctacggcta tcgaaatttc cataactttc gagcaagaat ttatctcatt
      481 caaggcttga tcttccaagt aaaacaaaaa ccagttaagc actctgccta actggttatg
      541 actaaatttt cagttgtatc tttctctacc aacactaaat gtcgaagagc cttttttcgt
      601 ttatgaatgt aagaccgctt ctcgagccaa tgccaaacaa ccgagcgttc cagcgttgtc
      661 ttctaatgct ggggtcacga tatatttgct taactcaggt gtttcgacat acccattgac
      721 caattttgca aatgcgtcgt ggacgttttg cagcatatgg cgttgtttca tcacaccacc
      781 gccaaaaatg atcacatctg gagaaaacat caaggttgta ttgtaggcac attgagcaat
      841 ataggaggct tcgatttccc aataaggatc atcttctgca acctcttgtc cttttttccc
      901 taagcgtttt tcgatggcgg gtccagcggc catcccttct aaacagttgc catgaaatgg
      961 acagctgcca gcaaagtcat cttttggatg agggacgacg agcatatgcc ccatttctgg
     1021 atgactaaac ccttcgataa agcgcccttc ttgtaaggca ccgccgccaa cacccgtacc
     1081 gatcgtatag taaacgacac tgctcaatcc tttgccactg ccgaagacat actcaccata
     1141 ggcagccgca ttgacatctg ttgtccaaga aatcgggatc ggaaattctt ttttcatggt
     1201 accgatgaaa tcaaaatttt gccatgccaa tttcggggtc gaggtaatgt acccataagt
     1261 ggcggaatcg cgatggatat cgataggccc aaatgagcca atcccaatcc ctgctaaatc
     1321 tgcttggtat tgtttgaaga agtcaatgac taaaggcatt gtttcctcag gtgtcgttgt
     1381 cggaaagctc actctttcct tgatcgtcat ttcttcatcg ccaatggcac acac
//
LOCUS       DAFEZB010000171         1424 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFEZB010000171
VERSION     DAFEZB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.191"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(150..1337)
                     /locus_tag="LP859_002865"
     CDS             complement(150..1337)
                     /locus_tag="LP859_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL3030737.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFEZB010000172         1422 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFEZB010000172
VERSION     DAFEZB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.192"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..115)
                     /locus_tag="LP859_002866"
     CDS             complement(<1..115)
                     /locus_tag="LP859_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3030738.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="LP859_002867"
     CDS             246..698
                     /locus_tag="LP859_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030739.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="LP859_002868"
     CDS             712..1401
                     /locus_tag="LP859_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030740.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
BASE COUNT          499 a          201 c          278 g          444 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat tt
//
LOCUS       DAFEZB010000173         1422 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFEZB010000173
VERSION     DAFEZB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.193"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            87..1388
                     /locus_tag="LP859_002869"
     CDS             87..1388
                     /locus_tag="LP859_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030741.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          466 a          286 c          262 g          408 t
ORIGIN      
        1 cggtcccttt tgagcatgaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc
       61 acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa ctactacttg
      121 gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt aagaatatca
      181 agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct ttgtgtaaac
      241 agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc attcaactta
      301 ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac cactgtcgtg
      361 gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc tttatcgcta
      421 aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg acgacaattg
      481 ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct ctgatcccca
      541 tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa tttcgttcac
      601 tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact ggaaaattac
      661 tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg caatttgaac
      721 gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct tatgcctctc
      781 tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac attgtcaaac
      841 atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt gatcgaaacg
      901 atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt ctgatcaaac
      961 gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt tgggcgtatc
     1021 tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta cgaatcgcct
     1081 atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac actttcttta
     1141 aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata aaaaaagctt
     1201 ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca aatgccaaaa
     1261 tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc tttcgaagct
     1321 ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat gaatcaaaaa
     1381 acatctaaag tagaaaagga attctacctt agatgttctt at
//
LOCUS       DAFEZB010000174         1407 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFEZB010000174
VERSION     DAFEZB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.195"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            20..>1407
                     /locus_tag="LP859_002870"
     CDS             20..>1407
                     /locus_tag="LP859_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3030742.1"
                     /translation="MSTKTIYQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVEHL
                     ISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRIINK
                     IIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSKVI
                     SVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRYME
                     TYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLIER
                     MSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVWYA
                     MSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVRRF
                     LPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIMEAY
                     TKYLIEEPSRFI"
BASE COUNT          481 a          196 c          246 g          484 t
ORIGIN      
        1 ttttagtgat aaattggcag tgtcgaccaa gaccatttat caagatattg aattgttgga
       61 agattatgta gttaattacc aagtaacaat tgagcgatta ccttctttgg gtatcaaact
      121 cataggtgat aggaaaaacg ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa
      181 agatatttat tctccagaaa aaagacgtgt cgagatcatt aaagaagttt tgtttggtga
      241 taagcctgtt tctttaactc atttgtctca aagttttatg gttagtaaaa catcgttata
      301 caatgacctg agaattatca ataaaattat tggcagtgga gaagttaagt tgcagtcatc
      361 tgcagaagga attagtttta ctggtaatga acaagcaatt caacgagcaa tcaagcaact
      421 tgtattctat tatgcgaatg acatggaatc aaaaacattt tttcaagttt taaatatgtt
      481 gtttgattca aaagttatta gtgtcattca tgatttgcta ttcaatgatt acagtgaact
      541 gactgaaaag gtttctgatt actatgttcg gtcattgatt tctactttag ttatccaatg
      601 taaccgttta ctgaaaggtc atcatttaac tgttgaagaa gaattcctct tcaatagcat
      661 tcgctatatg gaaacgtaca ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt
      721 gaatcttcca ttttatgacg cagataaaga atatctatgt cgtcaactat ttgcacatcg
      781 tgtgacaaat aatttaaaaa ccagcaatga agagtacgct atgagcgttc ataaattgat
      841 tgaaagaatg agtgagattg aaaaaattga tttgactcaa aatgatcgat tatatcaatc
      901 cttattgtat catgtgccag caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa
      961 tccgttatta gaaaatatca aatctcaata ttcagaactc tttagcatag tatggtacgc
     1021 catgtccatc cttgaagaag aatttgatgt gactttaacg gacgatgaag tttccttaat
     1081 tttgattcat tttcagattg ctattgaaga tcagtccaaa gcaaataata tcatcattat
     1141 ttgccaatac ggtatttctt ctgcgcaatt aatttacaat aaagttcgac ggtttttacc
     1201 agcaaaagat aatgtagaaa tatcaacttt agataagttt catttagctg aaattgggga
     1261 agttgatttg attatttcct ctctttcctt tgatgcaggt ggtataccct tagtaagggt
     1321 atctccattg gtaagtaaaa cagattatat gaatattatg gaagcttata ctaaatattt
     1381 aatagaagaa ccttcgcgat ttattca
//
LOCUS       DAFEZB010000175         1381 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFEZB010000175
VERSION     DAFEZB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.196"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            58..1353
                     /locus_tag="LP859_002871"
     CDS             58..1353
                     /locus_tag="LP859_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030743.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          433 a          275 c          263 g          410 t
ORIGIN      
        1 agacaccctg aaatccttta gaattaattt ggctaccaat tcataggagg attcaagatg
       61 tctcatactc agcttatcaa agatacccta aatattctag acctaaatat ctatttcgag
      121 gaaaattgtt taactaaaga aaaatacaaa gggcaaattt gcttgattta caaaggaact
      181 cttctgtaca agccagaaga gtgttcccat tgtctttgtg tcgtcccctc taggatcatt
      241 cgttggggga cgacaaccgt tcggttgtta ctgaatgatg tgtctgagta tcgcacttat
      301 ctggagctga agaaacaacg atttaaatgt aagtcttgcc aaagaacatt cgtagcggat
      361 acctctgttg ctgaaaaaca ctgtttcatt agtcaaaaag ttcgttggtc tgtcattgct
      421 cgcttaaaag agaatacttc catgacggaa atcgctcgtc agaaaaatat ttccacttct
      481 tcggtctatc gtgtcatgaa gcgattctac cgcccattga atcctttcaa acagactttg
      541 ccaaaggtct tatgctttga tgaattcaaa tcggttcgaa gtgttgccag tgcaatgagt
      601 tttatcatga tggacggaca aagccatgca ttattggata ttgtcgaaaa tcgacgcttg
      661 ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc cgttcaattg
      721 attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt cccgaatgct
      781 cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt cttgaatcat
      841 cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg agggttaggg
      901 aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt aaactatgaa
      961 aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat tgccgatcga
     1021 ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga cattctttat
     1081 gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga cattagcggt
     1141 ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc acagatccaa
     1201 aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa ccacatcaaa
     1261 gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact acgaattatg
     1321 attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt tttcttcttt
     1381 c
//
LOCUS       DAFEZB010000176         1377 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFEZB010000176
VERSION     DAFEZB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.197"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1310
                     /locus_tag="LP859_002872"
     CDS             <1..1310
                     /locus_tag="LP859_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBL3030744.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          591 a          103 c          230 g          453 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttatggaat taaaaatatt aaacaggaat
     1261 ttatcgaaaa tggtagaaaa gaaatttata aaaggactta taaagattga ttatataata
     1321 tatgaaaagc tattataaaa gacattagta ttaaatagtt taaaaaaatg aaaaata
//
LOCUS       DAFEZB010000177         1374 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFEZB010000177
VERSION     DAFEZB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.198"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            33..1372
                     /locus_tag="LP859_002873"
     CDS             join(33..474,474..1372)
                     /locus_tag="LP859_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL3030745.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAFEZB010000178         1359 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFEZB010000178
VERSION     DAFEZB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.199"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..85)
                     /locus_tag="LP859_002874"
     CDS             complement(<1..85)
                     /locus_tag="LP859_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030746.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            313..855
                     /locus_tag="LP859_002875"
     CDS             313..855
                     /locus_tag="LP859_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030747.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1011..>1359
                     /locus_tag="LP859_002876"
     CDS             1011..>1359
                     /locus_tag="LP859_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL3030748.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          525 a          166 c          260 g          408 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa gttcttcgtg ttctcttaga
      181 cataaaaatt cctcctggta tgttttcttc tagtctacac accttaattt ttctgtctag
      241 ttaattgtag cctatccagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa
      301 aaggtgtgta gaatgttatt gaaagtagct ataaaatgct attgtgtaaa aaataaagga
      361 ggaataattt atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg
      421 agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa
      481 tccataagtt ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt
      541 aagccatcag attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt
      601 aaatttaaac tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta
      661 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
      721 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      781 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      841 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      901 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttcttgat gaattaaaaa
      961 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
     1021 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
     1081 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
     1141 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
     1201 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
     1261 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
     1321 atgaaaagac aaaggcttca tatcctttct taatgccag
//
LOCUS       DAFEZB010000179         1354 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFEZB010000179
VERSION     DAFEZB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.200"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..111)
                     /locus_tag="LP859_002877"
     CDS             complement(<1..111)
                     /locus_tag="LP859_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HBL3030749.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLF"
     gene            complement(122..1183)
                     /locus_tag="LP859_002878"
     CDS             complement(122..1183)
                     /locus_tag="LP859_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL3030750.1"
                     /translation="MPDWLIDYAEKEGLIAELKPKHERQNFLVQDDRLDHAVAFLWKD
                     PQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSID
                     LLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRA
                     GHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEMKVEPEAIMA
                     IHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKGCERVDG
                     TYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQE
                     IRKQKRAMLFQQGKQQERE"
BASE COUNT          345 a          284 c          217 g          508 t
ORIGIN      
        1 aaacaaccga tcttggattt caaaataccc gtcttttcgg gtggctttag agaagctatc
       61 ggcataagct ttcgcttgtg acggcttttc tgctaaaata acggttttca tcatgacgat
      121 ttcactccct ttcttgttgt ttgccttgtt gaaacaacat cgcacgtttt tgctttctga
      181 tctcttgttg gtggatttca tgcttcaatt gatcattcca atcctttttt acttcaggta
      241 caaattcatg ttcatgattt ttatagcctt tgtagtattg ttctgcttta taagagaaaa
      301 gaatctgagc attaatatcc cctttttctt gataaaaacg atcaaaatcg tacgtaccat
      361 ccacgcgttc acaacctttt aattcttttg cgacttctct acaaatatct ttcaaattaa
      421 tagcttctat tgaatcgtta acagaaagct gctgaccaaa agacgcattt actttgtgag
      481 cggaaaccag ttggttcgtt aattgcagat tgttttctgt cttgtgtatg gccataattg
      541 cttcaggttc aactttcatt tcttttgcaa cttcttcata aatgactttg tattcttttg
      601 gcacttgcca atcattagcg attcctcgtt cacaacgtac tttctgattc gtaaatggat
      661 caactgcgcc cattaattgt tctttttcgt agaaaatatg tcctgcacgg tcattatcca
      721 cacaaatctc aatggattga ggaaaggctt gtttctttct caattcagat actgcctcac
      781 caaaataatg actgatcaca tggtgtttta acccttccat agagactaac cacgtatcgt
      841 ttaactgatc acgattcagt gcagcatagc taagtaaatc aatactactc tcaaaaaatt
      901 ttaactgttt agggtcaccg atctttaggt taaatccatg attcgctgtc gaatttttat
      961 ctatatgctt atatgtgcct ctctcaccaa aacgttcata atcaatgaat gttccttgat
     1021 aactcgcccc tactgtctct tttgtctgtg gatctttcca taaaaaggcg accgcatgat
     1081 ccaaacgatc gtcttgaact aagaaatttt gccgttcatg ttttggcttt agttcggcga
     1141 tcaatccttc tttttctgca taatcaatca accaatcggg cagttttcgt tcttccttca
     1201 aataattcct tcctttttct gtgagtggtt gataatcaac ggggtgatct ttgtactttt
     1261 ctgtctcaaa atgaagattt tctactcgtt ctgttttgtc ttcattcttt agaataaaat
     1321 ctgtggctgc tttaaattgt tgcttagagt cttg
//
LOCUS       DAFEZB010000180         1354 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFEZB010000180
VERSION     DAFEZB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.201"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..111)
                     /locus_tag="LP859_002879"
     CDS             complement(<1..111)
                     /locus_tag="LP859_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HBL3030751.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLF"
     gene            complement(122..1144)
                     /locus_tag="LP859_002880"
     CDS             complement(122..1144)
                     /locus_tag="LP859_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL3030752.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT          347 a          286 c          216 g          505 t
ORIGIN      
        1 aaacaatcga tcttggatct caaaataccc atcttttcgg gtagctttag aaaagctatc
       61 ggcataagcc ttcgcttgtg acggtttttc tgctaaaata acggttttca tcatgacgat
      121 ttcactccct ttcttgctgt ctaccttgtt gaaacaacat ggcacgtttt tgctttctga
      181 tctcttgttg gtagatttca tgcttcaatt gatcattcca atcctttttt acttcaggta
      241 caaattcatg ttcatgattt ttatagcctt tgtagtattg ttctgcttta taagagaaaa
      301 gaatctgagc attaatatcc cctttttctt gataaaaacg atcaaaatcg tacgtaccat
      361 ccacgcgttc acaagctttt aattcttttg cgacttctct acaaatatct ttcaaattaa
      421 tagcttctat tgaatcgtta acagaaagct gctgaccaaa agacgcattt actttgtgag
      481 cggaaaccaa ttggttcgtt aattgcagat tgttttctgt cttgtgtatg gccataatag
      541 cttctggtgt gatcttttct tcttttgcga cttcttcata aatgactttg tattcttttg
      601 gcacttgcca atcattagcg attcctcgtt cacaacgtac tttctgattc gtaaatggat
      661 caactgcgcc cattaattgt tctttttcgt agaaaatatg tcctgcacgg tcattatcca
      721 cacaaatctc gatggattga ggaaaggctt gtttctttct caattcagat actgcctcac
      781 caaaataatg actgatcaca tggtgtttta acccttccat cgaaactaac cacgtatcgt
      841 ttaactgatc acgatttagt gcagcatagc taagtaaatc aatactactc tcaaaaaatt
      901 ttaactgctt agggtcaccg atctttaggt taaagccatg attcgctgtc gaatttttat
      961 ctatatgctt atatgtgcct ctctcaccaa aacgttcata atcaatgaac gttccttgat
     1021 aactcgcccc tactgtctca tttgtctgtg gatctttcca taaaaaggcg accgcatgat
     1081 ctaaacgatc gtctcgaact aagaaatttt gccgttcatg ttttggcttt agttcagcaa
     1141 tcaacccttc tttttctgca taatcaatca gccactctgg tagttttcgt tcttccttca
     1201 gataattcct tcctttttct gtgagtggtt gataatcaac ggggtgatct ttgtactttt
     1261 ctgtctcaaa atgaagattt tctactcgtt ctgttttgtc ttcattcttt agaataaaat
     1321 ctgtggctgc tttaaattgt tgcttagagt cttg
//
LOCUS       DAFEZB010000181         1333 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFEZB010000181
VERSION     DAFEZB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.202"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..500)
                     /locus_tag="LP859_002881"
     CDS             complement(<1..500)
                     /locus_tag="LP859_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030753.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAK"
     gene            complement(774..1034)
                     /locus_tag="LP859_002882"
     CDS             complement(774..1034)
                     /locus_tag="LP859_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030754.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          464 a          240 c          198 g          431 t
ORIGIN      
        1 cactttgcta gggggcgggc ttgccattct tccaattcag gaaggacagc gtcagtgata
       61 tctgaaatca tttcatggga aatatcaaag ccataaatag cttcgacggt tgcggcaata
      121 tctcgttgac tcattcctcg tgcatacatg gaaagaacct tcccttcgat gtcggagaca
      181 tctcgttttc tcttaggaat taactctggt tcaaaggaag cttcccggtc tctaggaaca
      241 tcaatagcta cttcaccaaa actggtttta agcgttttag ttccatagcc atttcgacgg
      301 ttatcgtgtt ccttaggctc tttagaatgg gcatcataac ctaaatgatt attcaattct
      361 ccttgaagca ttttttcaaa aaggggccca aacacatctt tcaaagcatc ttgcatgtca
      421 tcgacagatt caggttgata ggcattcaga atggattcag ctaacttttc ggcatcagga
      481 tttcttttct ttctagccat cgtgtaatca cctcttgatc ttattgtaga aaaagaaaaa
      541 ccgcgaagca accacctgcc taggattaat ggttacttac acggtttata ttacactctc
      601 atcattaaaa aagtatcttt cgatattact aaacaatgaa attatctatt tgaaatttaa
      661 atctgataat atattgagtg ttccaatgag ggctttgatc ctattatcca tatctttttc
      721 agttttaata ttgatacaca ttataagaaa aagaaacttt tggttaattt atttcatccc
      781 agtgcaaaat ataaaaaagt aaaatagacc ataggcaata gattaacaat taatataata
      841 caagcaaata ttctattcat tgtatttctg ctttcaaaaa gttctaatat tgaaagtata
      901 attccacaaa tgcctataat aagtatggga ataagattag aaaataacac cccgagccaa
      961 tctgtgaaaa ttactatatt tactaatatc cctattaaca tacctatcat atttattaaa
     1021 tcaatttttt tcattagata tttctccttt attaaatctg aagtacttat attaaaataa
     1081 ttctaaacta tttacctcaa aaagtgcaat aaattatttc tgaacaataa aatgatagtc
     1141 aaaacaaaaa aatattgtta atcgttcaga gccatccgag agtgtaaaat attttgtgta
     1201 aatgaaaaaa tccatacaaa aaaggaaggc gcttctgtag aataaagtta acgacaacca
     1261 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
     1321 gtcactaaag gcg
//
LOCUS       DAFEZB010000182         1318 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFEZB010000182
VERSION     DAFEZB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.203"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(62..280)
                     /locus_tag="LP859_002883"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="LP859_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="LP859_002884"
     CDS             complement(546..740)
                     /locus_tag="LP859_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030755.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="LP859_002885"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="LP859_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          468 a          218 c          180 g          452 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatat
//
LOCUS       DAFEZB010000183         1294 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFEZB010000183
VERSION     DAFEZB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.204"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1244
                     /locus_tag="LP859_002886"
     CDS             <1..1244
                     /locus_tag="LP859_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL3030756.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFEZB010000184         1290 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFEZB010000184
VERSION     DAFEZB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.205"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            80..>1290
                     /locus_tag="LP859_002887"
     CDS             80..>1290
                     /locus_tag="LP859_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL3030757.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKT"
BASE COUNT          436 a          253 c          246 g          355 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat
//
LOCUS       DAFEZB010000185         1250 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFEZB010000185
VERSION     DAFEZB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.206"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            247..1221
                     /locus_tag="LP859_002888"
     CDS             247..1221
                     /locus_tag="LP859_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL3030758.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          446 a          190 c          253 g          361 t
ORIGIN      
        1 gtgctattat tgagttatca aataaataaa ctgaaaaaag agcaaaaaaa taaagtcgag
       61 caacgctgga cacgttctcg acctttacgg aaactaatca cactagctcc gtaactcaaa
      121 ttcattatac gaataatttc aggtttcttc aagactagag attgctggtt atgaattttt
      181 tagagttacg tgaactctgg aaaattcttt ccaccaatct cttttttctt tgaggaggaa
      241 ctaaatttga gtaaaaaagg cacgatcata aatgagtggg aaaaattaac agccccctct
      301 aacaggcggt taagtgtccc ttttatggac acccccctaa aaattcagtg gtcaatcgac
      361 cgaattacca ttgttggaaa tcttaaagag aatatctatt atcacactac acatgatgtg
      421 ttgattttag attttgaaca gcttatgcgc ctaaatgaag gtaacggtta tttaagatct
      481 gttagtaata atggttggca gttgttagac caacatgaag agaatattgc ttatattgaa
      541 attttaaaat ggcaagaggg gaaagggcgt atagacttca atccgaataa gattagtcaa
      601 ttcttagcta gttccatgaa aaattttatt catgatttgt ttatcgaacc tcacttttca
      661 cgtgcagata ttgcttgcga cattatcgat gtacctgatg agtttattac acaatatcgg
      721 gtagttgatc cagtttcttt taagcctatt tatggacgga atggaaaatt agagacggca
      781 tattggggat ctcgctcttc tgaacgtcaa attcggatgt ataataaaaa actggaacaa
      841 gagaagaaaa gaaaaattgt gccaaaagaa atcgtgagtt ggtggcggtt agaattacaa
      901 ttaagacgag ggaaagcgac tgattggcat gctatggttt atgaaagctt agatagtttt
      961 gctagtcctc attacttgcc tgctgataca agtgtagctg acaaaatgat gattactgct
     1021 ttaacaacag aacatgatta ttgggggcaa attaatcgaa agacaaaata taaatatcga
     1081 aatttgctga agcaagaaag tcaaaatgat gaattgacga atcatttacg agaaaccttt
     1141 gcagaatcgg cagacgattt aaaaaaagaa ttagacacat ggcttctagg gctagacgta
     1201 acagaggagg aagaaaaatg acagaaaaac aaaagtattt tggtaagaaa
//
LOCUS       DAFEZB010000186         1231 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFEZB010000186
VERSION     DAFEZB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.207"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>1231
                     /locus_tag="LP859_002889"
     rRNA            <1..>1231
                     /locus_tag="LP859_002889"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DAFEZB010000187         1193 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFEZB010000187
VERSION     DAFEZB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.208"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="LP859_002890"
     CDS             <1..1076
                     /locus_tag="LP859_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL3030759.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFEZB010000188         1187 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFEZB010000188
VERSION     DAFEZB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.209"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(189..1181)
                     /locus_tag="LP859_002891"
     CDS             complement(189..1181)
                     /locus_tag="LP859_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL3030760.1"
                     /translation="MSLLTALFLLPINGFAYTINNEFNLGANEGSSQVANNQYILLHE
                     TANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQ
                     IELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGD
                     HTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNY
                     AHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYN
                     TSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          333 a          237 c          221 g          396 t
ORIGIN      
        1 tggttgttgc cacataaagc cataaacaat caaccagtag aatgtgtggc aacaaacctg
       61 ttatcgcata gcttggagtg tgactattat gggtgatagt gaagatatgc gataacatca
      121 ctattttatc gaatgatttt tatagttgtc aatatagtta tgtaatgctc ctcaacgagg
      181 agctattttt atcgtttagg aatatttaaa taccaacgtt tgtcatggaa atcttgcgca
      241 ccgcctttag tgtttccctc tggatcattc gttgcccgca tcatgacata gactttctta
      301 ttagggaagt tacgcatgtt aaaagataca tgatagccaa catttccaga agtattataa
      361 gcttgattta catctgatct ataaattcca tcagctctta ctcgagctaa ttctttccca
      421 gtattgtaat ccataatgaa gatatactcg tatttatagt tagcaatgtg ccatccagct
      481 acatgcaaat ttgcattttc gatttctcca aactgatcaa tgtgggcgta attcgttcca
      541 tctgtcagtg taggattagc tgcaccagct cgtgttggat caatgacagg cttgttttca
      601 gaagttgttg gattttcatc ggtaaatcca tgagctaaat catatgctaa tttttcttta
      661 cttacgccca tttcagaaag ataaccgtaa ggatctgtat gatcgcccca gatgttttgt
      721 gttacccata aatgcgattt gattcccggt tggttataag gagtgtccaa cgttaatgga
      781 ataccatatt tcattgctga atctctagcc aattcaacgt atgccttgta gtttttctca
      841 aacgttgctt tatcatgtgt gtgttgtaac tcaatctgca caggactgtt ggcattagca
      901 tacgaaccag caccgtactg tacataacca ggttgaccga cttgataaac aattccgccg
      961 tctcccacaa tataagcagt ataagcgcta gtccatgaac gttgcatata ctgcgcttca
     1021 ttgcgtcctg ttgctgtttc attagccgtt tcatgcagta aaatatactg attatttgct
     1081 acttgtgagc taccttcatt tgcgcccaaa ttaaattcat tgttgatagt ataggcaaat
     1141 ccgttaattg gcaataaaaa aagagccgtt aacaggctca tcgcagt
//
LOCUS       DAFEZB010000189         1185 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFEZB010000189
VERSION     DAFEZB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.210"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            332..>1185
                     /locus_tag="LP859_002892"
     CDS             332..>1185
                     /locus_tag="LP859_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL3030761.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVA"
BASE COUNT          415 a          175 c          275 g          320 t
ORIGIN      
        1 gagattatcc caatgaatat gacggcttac tcggagtgat accgagtaaa aaaagtcaga
       61 tgaatgagag taaatttatg tcaattttta atgacgtgaa tttacgataa gctattattt
      121 tgcgtgaagc aaaataaagc gtaaaaggag ttgagagcgt gacacgcttt cggctccttt
      181 tttcgttgtc acttgtgaca gcgaaatccc tcggagagcg tttggaattg tgacagtcat
      241 gtcacaatta ggaaaacgtt actctttgac aaagagtaac agaaaggttt acactctttt
      301 tattagcgac agcgcgaagg aggggtctaa cttggggttt agtatttcgt tcaaaaaggg
      361 aacaaagaat acaagcattt ctcataataa tcgtgagtta actgatagcc aaaaaatcaa
      421 tgactggcat aagcatattg atttcacaaa atcggatgaa aatatctatt tggaacaaac
      481 aaatattcgt gaaaaatatg aagaattatt cggtgaagcc gtagaaaaat ataatgcaaa
      541 gcagaaaaga gccgatagaa aaatcgagga ctatttagca aaggttcgca aggataaaaa
      601 attggaacca cagcgagaat ttatcgtgca aattggtact cttgatgatt ttcgtactac
      661 tcgtgatgat ggttcttcta ctggcatatc ggaacaacaa gcagaacaaa atcgagtgat
      721 tgccaacaaa attttagttg agtatttcaa agaatttaaa gaacggaatc ctagtttatc
      781 cgtctacaat gctgttattc ataatgacga agtaagtccg cacctgcatt tgaatattgt
      841 tcctgttgct gaagggtata aacggggtgt tcaaaagcaa ccaagtttta acaaagcctt
      901 gcaacagcag ggtttacgag cagataaaga gaatggtcga gtgatttgga ataattttag
      961 gaatcaggaa gttgagtcaa ttgaacgttt gatggcagat tttggttggg agcgtgagtt
     1021 agtcggtact aacaaaatca aagatgttca cgaatataaa gaaatcatga gcgagatttc
     1081 agaattgcgt caaacggccg taaaagagcg tgagagcgtt tctgatgagt tggggtatat
     1141 ttctaggcaa agagaagaaa acgagcgaga aaaggctctg gtggc
//
LOCUS       DAFEZB010000190         1166 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFEZB010000190
VERSION     DAFEZB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.211"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            703..1035
                     /locus_tag="LP859_002893"
     CDS             703..1035
                     /locus_tag="LP859_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL3030762.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          405 a          173 c          214 g          374 t
ORIGIN      
        1 aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata
       61 attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata
      121 ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt
      181 cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta attgtctgct
      241 atgcttgtga tgaattgatt ttcgttctgc acttttggat gataaatttg gacaattgaa
      301 aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca
      361 tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg
      421 caatggattt agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg
      481 ttaaaaatac gtgatattac gatgaatcta tgcctaagca caatgttata aaaatgtccg
      541 tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt
      601 ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa
      661 aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt
      721 ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg
      781 aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag
      841 aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca aataatttta
      901 aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac
      961 ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg
     1021 cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt
     1081 gaaaatattg tcgagacttc taaaggaact tagaatcact agaaaaataa ggagtagact
     1141 tgatctatca acaattcctt tcgggg
//
LOCUS       DAFEZB010000191         1146 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFEZB010000191
VERSION     DAFEZB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.212"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..444
                     /locus_tag="LP859_002894"
     CDS             <1..444
                     /locus_tag="LP859_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBL3030763.1"
                     /translation="ARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQYGI
                     FRIHQEVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFLQDN
                     GRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            450..1058
                     /locus_tag="LP859_002895"
     CDS             450..1058
                     /locus_tag="LP859_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL3030764.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          331 a          230 c          289 g          296 t
ORIGIN      
        1 gcaagacaat atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt
       61 tgtgcggtat tgggaaacag tgccgcgtta gctgttggcg aggtggacca aatcaggctg
      121 cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca
      181 gttataaccg ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca
      241 aaaaaaatat ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa
      301 gataacggcc gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt
      361 cgttatcccc gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg
      421 atcgtattag cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa
      481 tagtacacgg tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac
      541 cggttgacgg ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt
      601 tgaaggcaaa agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc
      661 gtcctaatcg tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga
      721 caaaggaaag ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg
      781 cttcaaaatc aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca
      841 cgggtgagct tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg
      901 cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg
      961 aaaacagtgg gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac
     1021 cataccccaa tagctatttt gatttccccg ttaaataaac ttttaaccgt tgcacggaca
     1081 aactatataa gctaactctt tcggcaggaa acccgacgta tgtaactggt tcttagggaa
     1141 tttata
//
LOCUS       DAFEZB010000192         1137 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFEZB010000192
VERSION     DAFEZB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.213"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            75..692
                     /locus_tag="LP859_002896"
     CDS             75..692
                     /locus_tag="LP859_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030765.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            696..>1137
                     /locus_tag="LP859_002897"
     CDS             696..>1137
                     /locus_tag="LP859_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030766.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVG"
BASE COUNT          413 a          167 c          253 g          304 t
ORIGIN      
        1 ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa
       61 gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta
      121 aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat
      181 caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag
      241 ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg
      301 gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt
      361 tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta
      421 ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa
      481 agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag
      541 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag tcatatgtag
      601 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
      661 attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta aaaataaaac
      721 aaagcggtgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
      781 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
      841 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
      901 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
      961 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1021 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1081 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtgggga
//
LOCUS       DAFEZB010000193         1100 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFEZB010000193
VERSION     DAFEZB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.214"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1090
                     /locus_tag="LP859_002898"
     CDS             <1..1090
                     /locus_tag="LP859_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL3030767.1"
                     /translation="NTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELT
                     LEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQ
                     TMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGT
                     KEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQH
                     CCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQS
                     LIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIF
                     DTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          403 a          205 c          181 g          311 t
ORIGIN      
        1 aaacacactc ctacggactg aattaacggc ttttttagat tacgaaaaat atgatcgcac
       61 tggttttaat tcaggtaatt cgagaaacgg ttcttacttt cgatcaatca aaaccgaata
      121 tggtgaatta acattggaaa tacctagaga tcgtaatggt gagtttaaac aacaaacttt
      181 accagcctac aaaagaacaa acgatacatt ggaaaccact attatccatt tattcgaaaa
      241 aggtgttacg atgtctgaaa ttgctgattt gatcgaaaaa atgtacggtc atcactatac
      301 tccacaaacc atgtccaaca tgactaaagt tctgactgaa gaagtaaatg cctttaaatc
      361 cagagcctta aatgataagt atgtcgctat ttttatggac gctacttaca ttccactaaa
      421 acgtcaaacc gtatccaaag aagcgattta tattgccatt ggtatacgag aagacggcac
      481 taaagaagta ctgagttatg cgattgctcc aactgaatca acatacgttt ggaatgagct
      541 gctacaggat attaactcca gaggagttca agaagtcttg ctttttatta cggacggctt
      601 aaaaggcatg aaagatacta tccatcaaat ttatcctaaa gcaaaatatc agcattgttg
      661 tatccatgta tctcgtaaca tcgctcataa agtacgtgtc aaagaccgaa aagaaatctg
      721 tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata ccttcttatc
      781 cggcatgatt gagaaatgga agaaaaacta tcctaaagtg acgcagtcac tcatagaaaa
      841 ccaagactta ttaacttttt atgattttcc acctagcatt cgtagaacca tttactcaac
      901 caatctaatc gagtctttca ataagcaaat taaaagatac agccgtagaa aagagcagtt
      961 tcaaaatgaa gaatcactag aacgctttct agtcagcatt tttgatacat acaatcaaaa
     1021 atttctaaac agaagccata aaggttttca acaggtaacc gatacattag tttcaatgtt
     1081 tactgagtaa ctaattattt
//
LOCUS       DAFEZB010000194         1080 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFEZB010000194
VERSION     DAFEZB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.215"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(492..>1080)
                     /locus_tag="LP859_002899"
     CDS             complement(492..>1080)
                     /locus_tag="LP859_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030768.1"
                     /translation="FISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSKDYK
                     EVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSAIRK
                     IMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAMVLN
                     QLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          355 a          201 c          173 g          351 t
ORIGIN      
        1 tatattataa attcacatga ttatcgaatt ttattaagtt agtttttctt gtaaatctct
       61 gttcccaatg aaactcgaga cacttatttt aaagaggaaa caatgtggta tttaaatagc
      121 cctcttgtat gggctactct gtgatactta ttagccatta aactatttta cccacatagc
      181 atatattttg aaatcgttca ttccgtaaat tcaactaaat atgcctaaaa aaataaaaaa
      241 tctaagatag aataagattt attcagtctt agatggattt atagcaactt tttcgtaaca
      301 actttaaaaa atacgaaaag aaagagctgt tttactctga ctacgttgct aaattgtcgt
      361 ttaatcattc atttaaacca aatctttttt tatagaattc gttttaatcc tcctgaatcc
      421 aaaaactatg aaaaccgtaa ttaaatagtt aagagttttt aaccaccata attctctcgg
      481 ctattttgaa atcagataaa ttttgatcgg acacatagtt gaataaacct accgttgtta
      541 cggaaggtaa atcgcatact tttcattcta gaggagaagg tctcgttgat cattaactga
      601 ttaagcacca tagcccagtt ttgaattcga ccacctgacc acttcgcgtg taattctttc
      661 gtgcgtaaat aaagtagttt taggagtgca ttctcattag agaacgctcc tttttttgtg
      721 acttttctga agctggagtg gacactttca accgcattgg tagtgtacat aatttttcga
      781 atggcactac cataatcaaa tagttgttca acatgtgcaa agttccgttt ccagacatct
      841 acagcaccag aataatgaga ccaccgattt tgaaaactgt caaaagcagc atgtgcagcg
      901 tttagagaag aagcaccgta gaactttttc atatctcggc agacttcctt atagtcctta
      961 cttggaatat agcgcaatgc atttcgaaca agatgaacaa tacagcgctg aacaattacc
     1021 gatggaaaga tcgcttttgc gccttcttcg aggccagaaa caccgtccat cgaaatgaaa
//
LOCUS       DAFEZB010000195         1071 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFEZB010000195
VERSION     DAFEZB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.216"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..950)
                     /locus_tag="LP859_002900"
     CDS             complement(<1..950)
                     /locus_tag="LP859_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL3030769.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLG"
BASE COUNT          316 a          267 c          178 g          310 t
ORIGIN      
        1 cccaaacctg aaataccagg attgccaacg tacacgactt ttccgccatg aaccgcatga
       61 atctcacttc ctggatgatc gactgaacca aagtctagtc catcatggaa cccgttaggt
      121 cggaattctc ctcctggatt tttcccaaaa agttgtcctc cactgaacga atttttatct
      181 aagctacttc ctgggaatgg attgccccag cttcctgtaa tcgtaccacc agttccgccg
      241 ccttctctcc agtgtttaat cgcaaactca tcgtactcca tcaatttatt tgtattaata
      301 atagacatac aatttttata ataggcactg tcttcagtaa ttcctgcggc ggcataccct
      361 cctcgtccta agatcaataa gcttgcgcca gggcttttat ttccgacaca agcattaaaa
      421 tttgattgtg tggctaccat aaagccgtaa taggagaagg cagcttttga gttgggaaac
      481 ttcatgtacc agccaccttc aatgccacca attccacgcg cagttccttt aggaaacaga
      541 cacccatcaa accacgtaat cccaaaataa ttgagatcat ttcttgcgga cgccgacgta
      601 ccaaacgctg attcataacc gtattgcgaa aggatcacac ttggtaataa tttatacttc
      661 actgctgcac ctacagcata ttgcgcaatc tcatcagaaa aagggcctct tcctttgatt
      721 tctttataaa tgggtaagcc ttccgaatat tctcctgtaa aagcttcgcc accactactt
      781 gaatcagaat tatcaataga acctgccgca agtattagaa aaagggtcac aaacacagtg
      841 atcaatatag taatcaaacc tgcagaagta ccaaacaccc acaaaaataa gcgccgcttt
      901 gcacgacgct taacatactg tttgattaaa ctttcatttt ggttttccat tattttcctc
      961 catcaaacaa ttttaattga cgtttgttcg gactaaaatg aacacgaatg gtttcttttg
     1021 agccaaaatt gatcacagcg tctccttgat tttgttgagg aatggattct a
//
LOCUS       DAFEZB010000196         1058 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFEZB010000196
VERSION     DAFEZB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.217"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(36..992)
                     /locus_tag="LP859_002901"
     CDS             complement(36..992)
                     /locus_tag="LP859_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030770.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DAFEZB010000197         1016 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFEZB010000197
VERSION     DAFEZB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.218"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            13..1014
                     /locus_tag="LP859_002902"
     CDS             13..1014
                     /locus_tag="LP859_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBL3030771.1"
                     /translation="MNTTDNRRLYERFLAVKLVLEGHTRKEAGHTIGRDEHTVSHYVK
                     NYCHNGIQGLVSKKQSGRPSRLTDEQEAELGQIVAYHTPEEVGFKSRANWTLAIVCDL
                     ILREWSYGYTQKGVADLLHRLGLSWTRPTYTFKKADSKKQQVFLEETFPSLKKKLLNS
                     DIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGTLDYEAGRIICTE
                     EGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEENARRLALIFLPP
                     YSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERPLEVIDRLCVRM"
BASE COUNT          285 a          259 c          253 g          219 t
ORIGIN      
        1 ttggaaaaag cgatgaatac gacagacaac cgccggttgt acgagcgttt cctggcagtc
       61 aagttggttc ttgaaggcca cacccggaaa gaagccgggc acaccatcgg ccgcgacgag
      121 catactgtca gccactatgt gaaaaactac tgccataatg gaatccaagg tttagtctcc
      181 aaaaaacaaa gcggacgacc ttcacggctg acagatgaac aggaagcgga actgggccaa
      241 atcgtcgcct accacactcc ggaggaagta ggtttcaaat cccgtgccaa ttggacattg
      301 gcgattgtgt gtgacctgat tctccgggaa tggagttatg gatatacgca aaaaggcgtg
      361 gcggacctcc ttcaccggct aggactaagc tggacccgtc cgacgtatac cttcaaaaaa
      421 gcggattcca agaaacaaca ggtatttttg gaagaaacct tcccgagcct aaaaaaaaaa
      481 ttgctgaata gcgatattga ccacctccta ttcgaggacg aaaccatgat ccgggattac
      541 caagccattg gctgcacctg gtttctccgc ggccaccagc gaaaaatccc cacttatgga
      601 caacacaaag gggtaaaatt gataggcacc ttggattatg aagccggtcg gatcatttgt
      661 acagaagaag gggcatacga cgcagcggcc ttcctccgtt ttctggagcg gacgttgcgg
      721 gaatacccca ccggaaaaat cgtgatggta ttggataatg cccttatcca ccacgccaag
      781 ctgatccagt cttttcttga agaaaacgct aggcgcctgg cactcatctt cctccctccc
      841 tacagccctc agttgaacct aatggaaggc gtttggaagt ggctcaagga atcggtcatc
      901 aacaatgttt tttttgacca cgtccagaaa atcaaacagt cggtccgcgg tttcttagcc
      961 gatgtaaacg agcgtcccct tgaggtgatt gaccgactgt gcgtgcggat gtaaag
//
LOCUS       DAFEZB010000198         1000 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFEZB010000198
VERSION     DAFEZB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.219"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            369..1000
                     /locus_tag="LP859_002903"
                     /pseudo
     CDS             369..1000
                     /locus_tag="LP859_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAFEZB010000199          987 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFEZB010000199
VERSION     DAFEZB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.220"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..903
                     /locus_tag="LP859_002904"
     CDS             <1..903
                     /locus_tag="LP859_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3030772.1"
                     /translation="LSVQGTTTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQ
                     LMRLNENQGYLKSVGINGWQLFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASS
                     MKGFIHDLFLDPHFSRAYIACDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWG
                     SRASERQVRLYNKKLEQERKKVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFA
                     SPHFLPIDIKPIDKIVIDGLIAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRET
                     FKESADELKKELDTWLLGLDVTEK"
BASE COUNT          323 a          150 c          234 g          280 t
ORIGIN      
        1 ttaagtgtgc aaggtacgac caccccccta aaattaaatt ggtcgattga tcgaattaca
       61 attgtcggaa aactgaatga taccatagct tattggacga aaaatggagt tgtttttgtc
      121 gatttcgagc aattaatgcg tttgaacgaa aatcagggat atttgaaatc agttgggata
      181 aacggctggc aattattcga tcaatatgga gaagatattg cgtatatcga aatacttaaa
      241 tttcatgaag gaaaaggtcg tattgatttt aatccgaata agataggcca gtttttagct
      301 agttccatga aaggattcat tcatgaccta tttttagatc cgcatttttc ccgggcgtat
      361 attgcctgcg atattgtaga tgtgccggat gactttatta ctcagtatcg tattgtagac
      421 ccgatttctt ttaagccaat ctacggccgt tctggcaagc tagaaacagc gtactggggc
      481 agtcgtgcca gtgagcgcca agtgcgactg tacaacaaaa agctagagca ggaacgaaaa
      541 aaagtaatcg tgccaaaaga aatcgatacg tggtggcggt tagaaatgca gctaagacgt
      601 ggcaaagcaa cggattggca tgcgatggtt caggaaagct tagatagttt tgcaagcccg
      661 cattttttgc cgattgatat aaaacctatt gataaaatag ttattgatgg tttgatagct
      721 gagccttcta attggtcgat aattgctaga catacaaaat ataaatatcg gaatttgctg
      781 aagcaagaaa gtcaaaatga tgagttaacg aatcatttac gagagacgtt taaggaatct
      841 gcagacgaat taaaaaaaga attggatacg tggcttttgg ggttggatgt gacagagaag
      901 tgataccgaa taagaaaagt cagatgagtg agagtggatt tgcgtcaatg tttagtgacg
      961 taaattcacg ataagcgaat attttgc
//
LOCUS       DAFEZB010000200          983 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFEZB010000200
VERSION     DAFEZB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.221"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>983
                     /locus_tag="LP859_002905"
     rRNA            <1..>983
                     /locus_tag="LP859_002905"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          306 a          194 c          284 g          199 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taa
//
LOCUS       DAFEZB010000201          959 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFEZB010000201
VERSION     DAFEZB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.222"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            38..>959
                     /locus_tag="LP859_002906"
     CDS             38..>959
                     /locus_tag="LP859_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3030773.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFL"
BASE COUNT          343 a          173 c          184 g          259 t
ORIGIN      
        1 atagttccga cgaaagaatt ataaggagtg agtacaaatg acctataacc atcttacacc
       61 aactgaactc gtaatgatag aagcttattt caatcaatca caacctgttt caaaagtagc
      121 caatctgtta caacgttctc gacaaactat ttataaggtc tatcgttttc ttaaatctgg
      181 tggaagcgca atgagctact ataaacaata taagaaaaac aaacgtaact gcggaagaca
      241 tccgattgtt ttacctgatg agcaacagga atacattcaa aaaaaggttg ttcaagggtg
      301 ggctcctgat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgcacact
      361 gtatcgaatg tttaaagata aaacctttga cactcacgat ttaccaatga agggaaaaag
      421 aaaaccaaac ggtcataaag aaaaaagagg aaaacaaggg tttcgccggt caatccgaga
      481 cagaaagaca gactataccc agtttgatca tgaatttggt cacctggaag gtgacactat
      541 cgtgggtgga aaacataaaa gcgcggtcat cacattagtt gaacggctat ctaaagtcat
      601 cattaccttg aaacccgaag gcagacaggc taaagacatt gaaaataggc tgaatcaatg
      661 gtttcgttgt gtcccaaaaa acttatttaa atccatcaca tttgattgtg gaaaagaatt
      721 ctcaaattgg aaaaagatta gtaataccaa cgatatttca atttattttg cggatccagg
      781 aacaccttct caacgaggat tgaatgaaca ttcaaatggc ttattacgta aagatggttt
      841 gtataaagct atggatttta atggtgtatc tgaaaccttt atccaatcgg tggcatcgaa
      901 acgaaatcat attcctagaa aatcattaca ctatcggaca ccaatggaag tatttttga
//
LOCUS       DAFEZB010000202          932 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFEZB010000202
VERSION     DAFEZB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.223"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(53..616)
                     /locus_tag="LP859_002907"
     CDS             complement(53..616)
                     /locus_tag="LP859_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3030774.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          286 a          178 c          140 g          328 t
ORIGIN      
        1 aatcactcta attctcccat aaatattata gattcctaaa gattaaaata gattattccc
       61 tattgtcttt taagtagcgg tagagtgttg ccttgctaat accagtcatt tccgtaattt
      121 cttttacgct ataaacctga cttttataca attttactgc tcgatcaaca tctttctggt
      181 tcactcgtgg acggccacct ttacgacctc tcgcacgtgc acttttcaaa ccttctttgg
      241 tacgttcaac aattaaatca cgttcgaact gactaaatgc ttggaagaca gtcatcatta
      301 agcgtccgtg aggcgtgctg gtatcgaagt tttctttcaa gctcaccagc ttaacatggt
      361 gttcttcaaa ataggtaaca aggtcaatca aatcctttgt actacgaccc aatcgagaaa
      421 aactttctac aattaccgta tctcccggac gaattttatc ttttaaacga tttagttggg
      481 gacgaaccgc tttggttccc gtcattttct cagttaggat ttcattacaa ttttgttcat
      541 tgagtaagtc taattgacga tttagttctt gtgaacgtgt actcactcgg gcataaccat
      601 aaatgtatcc actcatgctt ttcttccttt cgtactttaa ttctggcttg attgtatcat
      661 aaaagggagt tatagataca tagttaatgc aacaagttat gatacaaaat atgacctctt
      721 ttatttaata aaagaggtca tttcattttg tctcataatc gattgttttt gatacgagtg
      781 tgtatacgaa ataaacaaaa aaatcttaag taaatttcta gatacctaat ttaatcctaa
      841 ctagtgtatc ctaaagttaa taacgagttt atcacaatga aaattataaa atgctcccat
      901 attagatata acatgcaata tttgaattag aa
//
LOCUS       DAFEZB010000203          895 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFEZB010000203
VERSION     DAFEZB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.224"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            28..>895
                     /locus_tag="LP859_002908"
     CDS             28..>895
                     /locus_tag="LP859_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3030775.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKR"
BASE COUNT          309 a          155 c          169 g          262 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaa
//
LOCUS       DAFEZB010000204          880 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFEZB010000204
VERSION     DAFEZB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.225"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..767
                     /locus_tag="LP859_002909"
     CDS             <1..767
                     /locus_tag="LP859_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030776.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          327 a          139 c          158 g          256 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact
//
LOCUS       DAFEZB010000205          861 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFEZB010000205
VERSION     DAFEZB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.226"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..129
                     /locus_tag="LP859_002910"
     CDS             <1..129
                     /locus_tag="LP859_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3030777.1"
                     /translation="QNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT          306 a          150 c          107 g          298 t
ORIGIN      
        1 caaaatgaag aatcactaga gcgtttccta gtatccatct ttgatacata caatcaaaaa
       61 ttcttaaata gaagccataa aggcttccaa caagtgacgg atacattagc ttcaatgttt
      121 actgagtaac ccattatttt gcagaaggac gagttattta cacaaaatta ttgacgctcc
      181 ctccaaatta cttcatcaaa acattgatat aatttttatt atatcagtat ttattcttga
      241 tacataaatg agaaaataaa tactttaaat ctattgacta caagtagtca ttaagtcttc
      301 ataagatgta caatatgaca ttaaaaaaat aaaagcttac gaccatccgt taaatctgac
      361 actaaataat ggactgcttt cttttgtatt agcaggttac gttgactatg aatattcacc
      421 agattatttg gagaaacctc ttcaacacga gaacaatgat attaatttta gatttgtcta
      481 cgagtcaaaa agtaaaacat ggttagttga tcaattaagc acgataaaca caagcttcca
      541 tgaattacct aaacctgaaa atgaagttga gatggtcatt aaaatcttct aaaaaagaag
      601 cgttgaaggg gaaatattcc tttcaacgct cttttttata tgcttagaaa cataaataaa
      661 ttgtatactt tctttttagc ccctattact tttcacgaaa aatatctttt tttcgacgct
      721 agcgtttctc tacttctaat actgtacaca gtttttgtat tattgaaaaa ttactcatta
      781 tttagcatta atccacatcg ttttatcaat cgaccttcat actaaactac tttaaagtca
      841 tcttccttct cgtttttcat t
//
LOCUS       DAFEZB010000206          820 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFEZB010000206
VERSION     DAFEZB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.227"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            19..>820
                     /locus_tag="LP859_002911"
     CDS             19..>820
                     /locus_tag="LP859_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3030778.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          252 a          141 c          185 g          242 t
ORIGIN      
        1 ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct caacttgacg
       61 agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa aaagaaagac
      121 tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag tcgagacggg
      181 gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga tcaacggggg
      241 ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga aaatatcgcc
      301 gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc agacggctcg
      361 attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc ggatttggtt
      421 ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct tagtgtttcg
      481 atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg agaaatcttc
      541 aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg aaaaacgtta
      601 tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt cgtcactcaa
      661 aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa gtttgctttt
      721 cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt ggatcaggag
      781 gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAFEZB010000207          789 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFEZB010000207
VERSION     DAFEZB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.228"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..782
                     /locus_tag="LP859_002912"
     CDS             <1..782
                     /locus_tag="LP859_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3030779.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DAFEZB010000208          763 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFEZB010000208
VERSION     DAFEZB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.229"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(237..716)
                     /locus_tag="LP859_002913"
     CDS             complement(237..716)
                     /locus_tag="LP859_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030780.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a          102 c           85 g          315 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttatattt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DAFEZB010000209          729 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFEZB010000209
VERSION     DAFEZB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.230"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..696
                     /locus_tag="LP859_002914"
     CDS             <1..696
                     /locus_tag="LP859_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030781.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          243 a          150 c          128 g          208 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatcca
      721 cttgatctc
//
LOCUS       DAFEZB010000210          683 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFEZB010000210
VERSION     DAFEZB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.232"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            182..529
                     /locus_tag="LP859_002915"
     CDS             182..529
                     /locus_tag="LP859_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030782.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQI
                     GVIYRNKSKGKEL"
     gene            533..>683
                     /locus_tag="LP859_002916"
     CDS             533..>683
                     /locus_tag="LP859_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030783.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESF"
BASE COUNT          261 a           73 c          146 g          203 t
ORIGIN      
        1 tatgaattga ttttgaaaaa taataaaatt gactctccga ttattatccc taagcacgcc
       61 gttaaatata tttatgcggt agctgaaagt agtggagaag ctaatgactg atttgtcaga
      121 aaagatgacc ataaaaaatc ttgctgatga attaggtgta tcgaaaatta aagaatcgga
      181 aatggtggca gttttggaaa tgaggtgtaa catggataaa attgatttaa gagaagagac
      241 agttgataga attgaattga tgtatgaacg attagctgat tatttgaatt tgccggatga
      301 agataattat agtgaaatgc ttgagagaat gatggacgtt ctcaattcta caatcaatgt
      361 aggcctagca aattcttctt tgattactga taaatttgtt tacggtaaaa tagccgaaac
      421 agaagatgaa tctgaaaagg aattttggct gaatatttta acttatgtta tgcaattaaa
      481 tcaaattggt gttatttatc gaaataaatc aaaaggcaag gagctgtaaa tcttgggaat
      541 gactttagca gatattaaag cacaagaaga aaaattgaaa aaagaacagg caaaacttcg
      601 tcaacagaaa aaacgattga ttgagaagga aaatgctgaa atgggtaagg cacttcggaa
      661 gcattttgga tttgaatctt ttg
//
LOCUS       DAFEZB010000211          683 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFEZB010000211
VERSION     DAFEZB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.233"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            83..683
                     /locus_tag="LP859_002917"
                     /pseudo
     CDS             83..683
                     /locus_tag="LP859_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          259 a          118 c          113 g          193 t
ORIGIN      
        1 tccatgttcc ccactcctca agtgttttct ttagtatacg cctataggag gaaaaacgaa
       61 tacaatttaa ctcaatttat ataataggaa aaatttagcc gaatctgaag gaattagtag
      121 agttactcta tatcaatgga aaaacacggt tcttggaaag gattttccat tgcgtatgac
      181 tttaaaaaat caggaaaaac aaaaagagct acttttaaag gaagtagaag acttacaaaa
      241 acaagtacac caattacagc tcgaaaaagc attactcgaa ggagcggctg aactgctaaa
      301 aaaagaaaaa ggcgtcaatc ttctttgtct atcaaatcaa gaaaaaacga tattgattga
      361 tgccttacga aatcagttca ccttgaaaga gcttcttcaa cagctccagt tgcctaaaag
      421 cagctatttt tatcaaaaac aagcacttga gaaaccagat aagtattaca aagaacgtca
      481 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattcacca
      541 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
      601 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
      661 ttctcctgca caccccaatt tat
//
LOCUS       DAFEZB010000212          680 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFEZB010000212
VERSION     DAFEZB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.234"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(205..>680)
                     /locus_tag="LP859_002918"
     CDS             complement(205..>680)
                     /locus_tag="LP859_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL3030784.1"
                     /translation="RGYNSTKNAYFYGLKIHIIVTKTGFPVTYSITDPSVHDVKVLET
                     LSEEANLPNILGDKGYVSHKIHEKLALKGITVLVPSRKSMDKLGKIDHRLLTRQRKAV
                     ETVFSSLERLGFQNFKSRSIQGLESKFESILLSYSILLSRAQQHFEGTLRYSLGY"
BASE COUNT          215 a          118 c          112 g          235 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taacatttcc atttcattta
      121 cctctttttc gtttaattat actaaagttc tatccaagcg acaagctgtg tataataaaa
      181 atatacccgt ggtgctagtt aattttaata ccctaaagaa tatctcaaag tcccttcaaa
      241 atgttgctgt gctcggctta aaagaatact gtacgataat aatatgcttt caaacttact
      301 ttctagccct tgaatagaac gtgatttgaa attttgaaaa cctagtcttt cgagagaaga
      361 aaaaacagtt tctactgctt ttctttgtct agtaagtaat cgatggtcta tcttccctag
      421 tttatccatg ctttttcgtg acggaactaa gactgtaatg cccttaaggg caagcttttc
      481 atggatttta tggctgacat agcctttatc tccaaggata tttggaaggt ttgcttcttc
      541 cgataaagtc tctaaaactt tcacatcatg aacactagga tccgtgattg aataagtaac
      601 tggaaagcct gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt
      661 cgttgagtta tagcccctac
//
LOCUS       DAFEZB010000213          662 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFEZB010000213
VERSION     DAFEZB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.235"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            135..401
                     /locus_tag="LP859_002919"
     CDS             135..401
                     /locus_tag="LP859_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL3030785.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            428..>662
                     /locus_tag="LP859_002920"
     CDS             428..>662
                     /locus_tag="LP859_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL3030786.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQRE"
BASE COUNT          236 a           97 c          139 g          190 t
ORIGIN      
        1 taactttatt tacaaatata tattatatcc atgaagccca atcttcttta atagaagatt
       61 gggctatttt tatggcaaag gagggaaaag aaatgtttca aaataaagga taacgttatg
      121 tcacaaaggg agtaatggac agtttgccgg tggaattaca agctttatgt tggaatctaa
      181 tcgatcaaaa cgttcaaaag caatttcctt tagactatct tcaaattttt gaattttcta
      241 cggaaaaagg aaatcaaaag ttggttcatc gtcaagaaga accggaagaa agaaaagagt
      301 atctgatttc acctaagctt cgtttgaaat cagtgtctca aaaaatatgg gccattgatt
      361 cgggtgagta tcaaacgatg ttattaccag aagaatacta gtatctaaaa gaaaggaacg
      421 aaaggacttg ttagaaacgg ctatcgaagt actggaaaag tgtgcacagc tggtcactgc
      481 ttctgaagag tggggctatg aatccgttac gatggaaaaa gaggaaattg aaatgggtat
      541 gcttcctgaa gatgttcatc taccacgttt agtgatgaca catctttata tttattgcgc
      601 accagaagat ggaaaagact atgtagttta tttcattaca gatattacca gccagcgaga
      661 at
//
LOCUS       DAFEZB010000214          630 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFEZB010000214
VERSION     DAFEZB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.236"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>630
                     /locus_tag="LP859_002921"
     rRNA            <1..>630
                     /locus_tag="LP859_002921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DAFEZB010000215          611 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFEZB010000215
VERSION     DAFEZB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.237"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(324..>611)
                     /locus_tag="LP859_002922"
     CDS             complement(324..>611)
                     /locus_tag="LP859_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030787.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          210 a           99 c           94 g          208 t
ORIGIN      
        1 gacatttggg ttaaattgtg agatgataat aaagaagagt ttaaagcgca ccccaagcca
       61 cttccccata agtgtgttac gctttaaact cttttatatt tgaagctatt aaaaggcata
      121 ccatattttt gaaaaaaagt gagaaaaaag gcttacaatt ggagtggtag ttaattagtg
      181 acttattttt gattttatag cactgatact ataaaatata gatatcatca tattacacaa
      241 tcttaatact aacttaaaaa atatctcctt tcacaagtat ggtgataaaa ttcgttccgg
      301 gctacctttt taggtagcct actttaatct ttgtatcttt ctggatcaac gaaagtatac
      361 tttatatagt cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt cacgatatca
      421 aacaaaaagt atacatcctt cttcattcta gttttcgcag caggaatttt gaaatagttc
      481 ttattagaat agtagagatt gattaataag ctatcttcga ttgctaaaaa gaaaacttct
      541 gaattccata ctttataaaa atctttgata aatctattcg aagggtcaaa tttaaaccat
      601 aattgtgtct c
//
LOCUS       DAFEZB010000216          599 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFEZB010000216
VERSION     DAFEZB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.238"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            94..>599
                     /locus_tag="LP859_002923"
     CDS             94..>599
                     /locus_tag="LP859_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3030788.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPV
                     IDPTRAGAA"
BASE COUNT          204 a          103 c          123 g          169 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaatcc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa ctacttcaga tgataaacca gtcattgatc caactagagc aggtgcagc
//
LOCUS       DAFEZB010000217          592 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFEZB010000217
VERSION     DAFEZB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.239"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..579
                     /locus_tag="LP859_002924"
     CDS             <1..579
                     /locus_tag="LP859_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3030789.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          203 a          105 c          122 g          162 t
ORIGIN      
        1 ggaatcaaat cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc
       61 ggttatcttt ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt
      121 tttacggatg ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca
      181 gtaattgatc caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac
      241 gcccacgttg atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac
      301 attgctaatt ataaatacga gtatattttc attatggact acaatactgg aaaagaacta
      361 gctagagtaa gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta
      421 ggaaatgttg gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac
      481 gtcatgatgc gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt
      541 catgacaagc gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DAFEZB010000218          548 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFEZB010000218
VERSION     DAFEZB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.241"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..>548)
                     /locus_tag="LP859_002925"
     CDS             complement(<1..>548)
                     /locus_tag="LP859_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL3030790.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DAFEZB010000219          528 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFEZB010000219
VERSION     DAFEZB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.242"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="LP859_002926"
     tRNA            complement(5..90)
                     /locus_tag="LP859_002926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LP859_002927"
     tRNA            complement(107..178)
                     /locus_tag="LP859_002927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LP859_002928"
     tRNA            complement(190..262)
                     /locus_tag="LP859_002928"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LP859_002929"
     tRNA            complement(280..361)
                     /locus_tag="LP859_002929"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LP859_002930"
     tRNA            complement(369..441)
                     /locus_tag="LP859_002930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LP859_002931"
     tRNA            complement(445..517)
                     /locus_tag="LP859_002931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAFEZB010000220          527 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFEZB010000220
VERSION     DAFEZB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.243"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            258..527
                     /locus_tag="LP859_002932"
                     /pseudo
     CDS             258..527
                     /locus_tag="LP859_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          181 a           94 c           76 g          176 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataac
//
LOCUS       DAFEZB010000221          525 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFEZB010000221
VERSION     DAFEZB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.244"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..134
                     /locus_tag="LP859_002933"
     CDS             <1..134
                     /locus_tag="LP859_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3030791.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          220 a           75 c           56 g          174 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atacaaacaa aaataaataa agtcggattt ttctgataat
      181 tcaacgcaaa aaaacgaaca atttttcaat aaatcagtct tctaaaatag ataacctaaa
      241 aaaaacgaac aaacaaataa aatgactgtc cttcattcac taaacatatt tttatttttt
      301 atattgttaa aaattcacaa ttatttttta atagaattat ctgttttcca aattttataa
      361 cataaaaaac cttttttaac ggcatttata tgctttttca gaaattaact gatgatttca
      421 aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg tggaggctct
      481 actatgattg aagaatatat tgagaaaaat attttaagac agctg
//
LOCUS       DAFEZB010000222          524 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFEZB010000222
VERSION     DAFEZB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.245"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="LP859_002934"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="LP859_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL3030792.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>524)
                     /locus_tag="LP859_002935"
     CDS             complement(349..>524)
                     /locus_tag="LP859_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030793.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          153 a          115 c           90 g          166 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaa
//
LOCUS       DAFEZB010000223          514 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFEZB010000223
VERSION     DAFEZB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.246"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..132)
                     /locus_tag="LP859_002936"
     CDS             complement(<1..132)
                     /locus_tag="LP859_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL3030794.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAFEZB010000224          509 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFEZB010000224
VERSION     DAFEZB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.247"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            81..>509
                     /locus_tag="LP859_002937"
     CDS             81..>509
                     /locus_tag="LP859_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030795.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQ"
BASE COUNT          193 a           93 c           91 g          132 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacag
//
LOCUS       DAFEZB010000225          504 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFEZB010000225
VERSION     DAFEZB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.248"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DAFEZB010000226          500 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFEZB010000226
VERSION     DAFEZB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.249"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>500
                     /locus_tag="LP859_002938"
     CDS             <1..>500
                     /locus_tag="LP859_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HBL3030796.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DAFEZB010000227          488 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFEZB010000227
VERSION     DAFEZB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.250"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            43..>488
                     /locus_tag="LP859_002939"
     CDS             43..>488
                     /locus_tag="LP859_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBL3030797.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGY"
BASE COUNT          175 a           87 c           82 g          144 t
ORIGIN      
        1 gcatatagta gtctcaaaac tcccaagaaa tgaggactac atatgcgttc atcaaaatat
       61 actgaacatt atacagatac ttttataaca ttcaaggaaa ttatgagaac tgtttctaac
      121 atataccaca attgtgtacc agataagatt aaaaaccgaa gaaatactga tcaactgaag
      181 caacgcgata cagtgattat tgcttgtgtt atatggggca taattaatgg ctatactagc
      241 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
      301 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      361 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      421 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      481 ggctataa
//
LOCUS       DAFEZB010000228          487 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFEZB010000228
VERSION     DAFEZB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.251"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..414)
                     /locus_tag="LP859_002940"
     CDS             complement(<1..414)
                     /locus_tag="LP859_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030798.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          162 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaaca
//
LOCUS       DAFEZB010000229          487 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFEZB010000229
VERSION     DAFEZB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.252"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..>487)
                     /locus_tag="LP859_002941"
     CDS             complement(<1..>487)
                     /locus_tag="LP859_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBL3030799.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAFEZB010000230          486 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFEZB010000230
VERSION     DAFEZB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.253"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..>486)
                     /locus_tag="LP859_002942"
     CDS             complement(<1..>486)
                     /locus_tag="LP859_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030800.1"
                     /translation="LYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLN
                     QCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMR
                     WTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTS
                     L"
BASE COUNT          127 a          107 c           84 g          168 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagaga
//
LOCUS       DAFEZB010000231          436 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFEZB010000231
VERSION     DAFEZB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.254"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT          142 a           59 c           80 g          155 t
ORIGIN      
        1 gatcgttacc ttaaaatgcc ttacttcttt gaccaagcaa aggtcatttg cctttgtcac
       61 tacgatgggg tacgtgattt taggcgttaa aaagtagtga ttatatcaac tttacgagta
      121 aatataaatt tagacatgaa actgaattat ccaatggaaa atcatggttt ataatctaga
      181 aaaattatag taatgattgg attatatata ttctttcgta ttttcgtaca actgtagctt
      241 gtcaaaattg agaaatttaa ccgattaggt actttacatc gttatttggg taatttctaa
      301 aacatgaagc ttaccaacga ataaactgag actatcttat taattgatca atataagaaa
      361 atatctttga ggagatgatg tactatgttt tcgtctatcc acgattggtt aggtggtctt
      421 ttcggcggtg gaggta
//
LOCUS       DAFEZB010000232          430 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFEZB010000232
VERSION     DAFEZB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.255"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>430
                     /locus_tag="LP859_002943"
     CDS             <1..>430
                     /locus_tag="LP859_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBL3030801.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAFEZB010000233          415 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFEZB010000233
VERSION     DAFEZB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.256"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..339)
                     /locus_tag="LP859_002944"
     CDS             complement(<1..339)
                     /locus_tag="LP859_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030802.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAFEZB010000234          392 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFEZB010000234
VERSION     DAFEZB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.258"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            2..370
                     /locus_tag="LP859_002945"
     CDS             2..370
                     /locus_tag="LP859_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL3030803.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          127 a           59 c           87 g          119 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt tt
//
LOCUS       DAFEZB010000235          392 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFEZB010000235
VERSION     DAFEZB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.259"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            1..392
                     /locus_tag="LP859_002946"
                     /pseudo
     CDS             1..392
                     /locus_tag="LP859_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          129 a           67 c           75 g          121 t
ORIGIN      
        1 tggaatgagt tgttacagga catttactcc agaggggttc atgatgtctt actgtttatt
       61 accgatggct taaaaggcat gaaagatacg attcaccaga tttatcctaa agctaaatac
      121 caacattgct gtgttcacgt ttcccgtaat attgctcata aagtacgtgt caaagatcga
      181 aaagaaatct gtgatgattt taaggctgtt tatcaagcta gctcaaagga agaggcaaat
      241 acctttttag ggagtatgat tgagaagtag caaaaaactt atcctaaagt gacgcagtca
      301 ctgataaaaa atcaagattt attgactttt tatgagtttc cgcctggtat ccgccgaagc
      361 atttactcaa ctaatttaat cgaatctttc aa
//
LOCUS       DAFEZB010000236          366 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFEZB010000236
VERSION     DAFEZB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.260"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            80..>366
                     /locus_tag="LP859_002947"
     CDS             80..>366
                     /locus_tag="LP859_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL3030804.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          129 a           59 c           76 g          102 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga
      301 acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa
      361 ctgtcg
//
LOCUS       DAFEZB010000237          365 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFEZB010000237
VERSION     DAFEZB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.261"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>365
                     /locus_tag="LP859_002948"
     CDS             <1..>365
                     /locus_tag="LP859_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030805.1"
                     /translation="VVEKENTRKPYTEVENGVSYIVPKGKRRQATLDAMKTNYVHELE
                     RFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYKTLPDKRS
                     EWHYGDEKRTKLSPYTREKL"
BASE COUNT          144 a           57 c           81 g           83 t
ORIGIN      
        1 gttgttgaaa aagaaaatac gcgtaaacct tacacggagg ttgaaaatgg tgtgagttat
       61 atcgtaccga aaggtaagag aagacaagca acattagacg caatgaagac caattatgta
      121 catgagttag aacgtttttc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat
      181 ttattgacgc ggaaaagtga tttgcgtaac accttaacaa aagtggtaaa agagccagcg
      241 cgttacgatc gacacgcaat gaacttatta aatgaagtat ataaaaccct acctgacaaa
      301 cgttcggaat ggcattatgg ggatgaaaaa cgaaccaaac tttcaccata cacaagagag
      361 aaact
//
LOCUS       DAFEZB010000238          364 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFEZB010000238
VERSION     DAFEZB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.262"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>364
                     /locus_tag="LP859_002949"
     CDS             <1..>364
                     /locus_tag="LP859_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030806.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDASEAIKNKKDLPKDTKYTWKEKVDVSTAGNKKGTVV
                     VTYPDGSSDEVEVDVTVT"
BASE COUNT          153 a           55 c           79 g           77 t
ORIGIN      
        1 tgccagaaga taaacttccg gtgttaaaag caaaagatgg ttacacagat gcaaaatggc
       61 cagaagaagc aacccaacca attacggcag atgatacaga atttgtatca agcgcaacaa
      121 aactggatga taaatctgac gctgacaagt ataatcctga aggtcaaaaa gtgactacag
      181 aattgaataa agaaccagac gcatctgagg caattaaaaa taagaaagat ttaccaaaag
      241 atactaagta tacttggaaa gaaaaagtag atgttagcac agctggaaat aaaaaaggta
      301 ctgttgtagt gacatatcca gatggatcat ctgatgaagt tgaagtggat gttacagtga
      361 caga
//
LOCUS       DAFEZB010000239          362 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFEZB010000239
VERSION     DAFEZB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.263"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..>362)
                     /locus_tag="LP859_002950"
     rRNA            complement(<1..>362)
                     /locus_tag="LP859_002950"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAFEZB010000240          362 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFEZB010000240
VERSION     DAFEZB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.264"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>362
                     /locus_tag="LP859_002951"
     CDS             <1..>362
                     /locus_tag="LP859_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL3030807.1"
                     /translation="MQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSY
                     WQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSW
                     FESIKDILKSIDPGGVMLA"
BASE COUNT          130 a           44 c           82 g          106 t
ORIGIN      
        1 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
       61 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      121 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      181 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      241 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      301 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      361 gc
//
LOCUS       DAFEZB010000241          361 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFEZB010000241
VERSION     DAFEZB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.265"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            1..361
                     /locus_tag="LP859_002952"
                     /pseudo
     CDS             1..361
                     /locus_tag="LP859_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           63 c           65 g           87 t
ORIGIN      
        1 ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta gctggaaagc cacgtgtaga
       61 aatcattcga gaatatgaac taacggcttc agcatttgac aaatgggtaa agcaatctaa
      121 aacgagtggt tcattcaaag aaaaagataa tcttacgcct gaacaaaaag aattgttaga
      181 actacgtaaa agaaaccagc aattagaaat ggaaaatgat attttaaagc aagcagcgct
      241 gatattcgga cgaagagaca agtaatcgat gcgaataagc atctttaccc tatatcagcg
      301 atgtgcagaa tattaggtct atcacgtcag tcctattatt atcaatcaaa accaaagaaa
      361 g
//
LOCUS       DAFEZB010000242          348 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFEZB010000242
VERSION     DAFEZB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.266"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..317)
                     /locus_tag="LP859_002953"
     CDS             complement(<1..317)
                     /locus_tag="LP859_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030808.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTT"
BASE COUNT          102 a           73 c           35 g          138 t
ORIGIN      
        1 tttgttgtaa cctcctcata atcagttagt actataaagg tatctttgtt tttatccaaa
       61 aaatctaata aatcttttgt agaagatatc tcttcatcaa cttcaccata ataaatacct
      121 tcgataattg ttgctatatc tgaaaaacta atttttactg gacatttatc gtctaccgca
      181 ttcaataccg acaaaattgc ttctttttct gcaatctctc gtttgaattt ctttggagct
      241 aatcgcatga cactcaagtc attttcaagc ttttcaatcc atgcagtttt actcattttc
      301 gcaaattttt gaatcattcc tctaccacct ttgctatcta ctaaatta
//
LOCUS       DAFEZB010000243          345 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFEZB010000243
VERSION     DAFEZB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.267"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT          113 a           65 c           56 g          111 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttct
//
LOCUS       DAFEZB010000244          338 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFEZB010000244
VERSION     DAFEZB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.268"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            123..329
                     /locus_tag="LP859_002954"
     CDS             123..329
                     /locus_tag="LP859_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL3030809.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          121 a           43 c           82 g           92 t
ORIGIN      
        1 agataaaatt gaacaactgg acgggcaaat tgatgccttg ctttcagaaa gagaaatatt
       61 gatagaagaa aacaagcgat tgaaaaagaa agtagcccaa ccaatgaagg gaggtggata
      121 gtatgaacaa tagaaccttt gatattttga aatggatcgc tatagtagtt atccccgctt
      181 tagcgacttt tgtcggtctt gtcggaaaag gtgttaactg gcggtacaca gatataacgg
      241 tgatgattat tacaggtttt ggagcatttc tagggagtat tttaggtgta tcaaatcgaa
      301 cgtataagaa aatttcatca gataaatagg aggataga
//
LOCUS       DAFEZB010000245          332 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFEZB010000245
VERSION     DAFEZB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.269"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>332
                     /locus_tag="LP859_002955"
     CDS             <1..>332
                     /locus_tag="LP859_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030810.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAFEZB010000246          326 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFEZB010000246
VERSION     DAFEZB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.270"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..293
                     /locus_tag="LP859_002956"
     CDS             <1..293
                     /locus_tag="LP859_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3030811.1"
                     /translation="VTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIK
                     RRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT          108 a           61 c           70 g           87 t
ORIGIN      
        1 ttgtgaccga taaagcacct tctcttggct ccgcctttag aaagttacag agtgtgggtt
       61 tatatactaa gacagagcac cgaactgtga agtatcttaa caatttaata gaacaagacc
      121 atcgacctat taaacgacga aataaatttt atcaaagtct ccgtacagcc tcttccacga
      181 ttaagggcat ggagaccatt cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct
      241 tcggcttttc ggtgtctact gaaatcaagg tattaatggg aataacagcc taagatattt
      301 ggagttcaga gagggcgcgt ttgatt
//
LOCUS       DAFEZB010000247          324 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFEZB010000247
VERSION     DAFEZB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.271"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT          105 a           75 c           50 g           94 t
ORIGIN      
        1 tgttcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag tatgccatca
       61 ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac cagctccatc
      121 tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct tcgttgtatc
      181 ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac aagtactctc
      241 ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa aaaaaacgaa
      301 aggctagtcg tttcataaaa aaaa
//
LOCUS       DAFEZB010000248          323 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFEZB010000248
VERSION     DAFEZB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.272"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="LP859_002957"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="LP859_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL3030812.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAFEZB010000249          321 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFEZB010000249
VERSION     DAFEZB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.273"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>321
                     /locus_tag="LP859_002958"
     CDS             <1..>321
                     /locus_tag="LP859_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030813.1"
                     /translation="KNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFM
                     IQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFT"
BASE COUNT          117 a           62 c           60 g           82 t
ORIGIN      
        1 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
       61 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      121 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      181 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      241 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      301 tcctaagaaa tatctttacc a
//
LOCUS       DAFEZB010000250          307 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFEZB010000250
VERSION     DAFEZB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.274"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            97..>307
                     /locus_tag="LP859_002959"
     CDS             97..>307
                     /locus_tag="LP859_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030814.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRL"
BASE COUNT          111 a           62 c           47 g           87 t
ORIGIN      
        1 caccagcatc ttttttattt tccaaaacat atagaaaaga caccttgaaa tcctttagaa
       61 ttaatttggc gaaccaaccc aaaaggagga atcaagatgt cttacactca ccttatcaaa
      121 gaaacattag atattctaga cttatcagtt acttttaacg aaaattgtct cacaaaagaa
      181 aaatacaaag ggcaaatctg tcatatttat cgtggaaact tgatttatac agcccaagaa
      241 tgcatccact gtaaacacca gattgcttca gatattgtgc gttggggtac aacaaccgtc
      301 cggttgt
//
LOCUS       DAFEZB010000251          302 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFEZB010000251
VERSION     DAFEZB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.275"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>302
                     /locus_tag="LP859_002960"
     CDS             <1..>302
                     /locus_tag="LP859_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBL3030815.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          117 a           48 c           68 g           69 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 ag
//
LOCUS       DAFEZB010000252          291 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFEZB010000252
VERSION     DAFEZB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.276"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>291
                     /locus_tag="LP859_002961"
     CDS             <1..>291
                     /locus_tag="LP859_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030816.1"
                     /translation="QQTVPAYRRTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYT
                     PQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMDATYIPLKRQTVSKEAIYIAI"
BASE COUNT           97 a           63 c           53 g           78 t
ORIGIN      
        1 caacagactg ttcctgctta tagacggacg aatgatacct tagaggagac cgtcattcac
       61 ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa aatgtatggg
      121 catcactaca cgccccaaac catgtccaac atgactaaag tgctgactga agaagtaaat
      181 gcatttaaag ttagaactct aaatgataag tatgtcgcta ttttcatgga tgctacttat
      241 attcctttaa aacggcaaac cgtctccaaa gaagcaatct atattgccat t
//
LOCUS       DAFEZB010000253          287 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFEZB010000253
VERSION     DAFEZB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.277"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>287
                     /locus_tag="LP859_002962"
     CDS             <1..>287
                     /locus_tag="LP859_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL3030817.1"
                     /translation="GFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQ
                     FKVVKQQLQSADTIIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSL"
BASE COUNT           94 a           54 c           60 g           79 t
ORIGIN      
        1 atggttttgg tcatttagtc gagttagatt cgcccgatat gtacgatgaa aattggaaac
       61 aatggtcatt agagcatttg cctattttcc ctacacatta tcactatcat gttcctaaag
      121 ataagaaaaa gcaattcaag gtggtgaaac agcaacttca atcggccgac accattatta
      181 ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg gacgatcatt caacaagcag
      241 gcgccgatca tggaaagata tttaaaagac tttggatcaa ctcctta
//
LOCUS       DAFEZB010000254          286 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFEZB010000254
VERSION     DAFEZB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.278"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..>286)
                     /locus_tag="LP859_002963"
     CDS             complement(<1..>286)
                     /locus_tag="LP859_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030818.1"
                     /translation="ISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVET
                     VFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSL"
BASE COUNT           84 a           51 c           50 g          101 t
ORIGIN      
        1 taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata gaacactgta
       61 agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg agttgaaatt
      121 ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc tttgttttcc
      181 aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg gaacggatat
      241 agtaatgccc ttaagggcaa gcttttcatg gattttatgg ctaata
//
LOCUS       DAFEZB010000255          286 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFEZB010000255
VERSION     DAFEZB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.279"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>286
                     /locus_tag="LP859_002964"
     CDS             <1..>286
                     /locus_tag="LP859_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030819.1"
                     /translation="VATYEDGSPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMN
                     TTDWYVRKDTVLSEFKSFYNNLNVVGGHPGGSSKLTQADNDFNNLHDKLG"
BASE COUNT           99 a           51 c           62 g           74 t
ORIGIN      
        1 tagtagccac atatgaggac ggtagccccg ttcaattttc aggagataca tttatcagtt
       61 ttaactcttt gaaccctgca ggaggaaaaa gcacggatct taaaggagaa tatgcccact
      121 atgacaaaat gaatacgaca gattggtatg tcagaaaaga tacggttctt tcagaattta
      181 aatctttcta caacaatctg aacgtggtag gtggccaccc aggaggaagc tctaagctga
      241 cacaagcgga taatgatttc aataatttgc atgataaatt aggaga
//
LOCUS       DAFEZB010000256          279 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFEZB010000256
VERSION     DAFEZB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.280"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT           78 a           65 c           37 g           99 t
ORIGIN      
        1 acgagattgt ctctgctttt ttcaacaaca actccaatcg ttgaataagc catttcttct
       61 tcctcccttc tctttctttt gatataatgt agtcgcaaat cgcaccactc attgaattgg
      121 cggaaaacaa ttcattgaaa aaaagagtcc tggacaatta cgttcaggac tcttttactt
      181 cttttattca cttaacaact aacattctgt gagtgaaata gactcttcaa tgcttttaca
      241 cgggaagtta ttgatcccct cttcattcat ttcccaagt
//
LOCUS       DAFEZB010000257          277 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFEZB010000257
VERSION     DAFEZB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.281"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            45..>277
                     /locus_tag="LP859_002965"
     CDS             45..>277
                     /locus_tag="LP859_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030820.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEG"
BASE COUNT          111 a           31 c           62 g           73 t
ORIGIN      
        1 caaatggaac aagaaagaca gtaaagacag taggaggaat agcaatgaaa caatatttag
       61 atcaatggaa agttatagaa ggatcattaa gagaagaacg aatcgaacaa cttcccgatt
      121 gtttagaaaa agagcatttg tttcagataa gagaaatgtt gagaaacgaa cagtttgatc
      181 caaatcaatt tttagtcgtt gagtactcag ctactggtgt gtactgttgt aatcatgtga
      241 agggtgaaaa atattttatc attcaagaat atgaggg
//
LOCUS       DAFEZB010000258          267 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFEZB010000258
VERSION     DAFEZB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.283"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(36..>267)
                     /locus_tag="LP859_002966"
     CDS             complement(36..>267)
                     /locus_tag="LP859_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030821.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          103 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaat
//
LOCUS       DAFEZB010000259          266 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFEZB010000259
VERSION     DAFEZB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.284"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            85..>266
                     /locus_tag="LP859_002967"
     CDS             85..>266
                     /locus_tag="LP859_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3030822.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           84 t
ORIGIN      
        1 tacctatgaa atattttcac gtgttaaagt attcctcgtt gaacacaaca aataaaactt
       61 tttaacgaat aaaggagatt tttcatgaca tcacttaaaa ctttactttt tggaacaact
      121 ttggctgccg gcgctgcatt cttcatggga acgactgctc atgcggacga agcttatacc
      181 gttcaatcag gtgatactct atcaacgatt tctcaaaaat acgttggtga taactccttg
      241 atcaatgcca ttgcagaatc aaattc
//
LOCUS       DAFEZB010000260          264 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFEZB010000260
VERSION     DAFEZB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.285"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>264
                     /locus_tag="LP859_002968"
     CDS             <1..>264
                     /locus_tag="LP859_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030823.1"
                     /translation="GIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQ
                     YTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRL"
BASE COUNT           99 a           53 c           44 g           68 t
ORIGIN      
        1 aggaattttt aagttcacag atattaactt agcgcgtgag gctaaaaatc gattgattca
       61 tgattatatc gatcaaccaa aatattcaaa agcttgcgca tcattggatg atggattcga
      121 agacgccttt caatataccg tacaaggaaa ttcccacaat cgactaaaga gtaccaatct
      181 aattgaacga ctgaatcaag aagtacgcag aagagaaaag attattcgca tcttccccaa
      241 tcaaacatca gccaatcgct taat
//
LOCUS       DAFEZB010000261          255 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFEZB010000261
VERSION     DAFEZB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.287"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>255
                     /locus_tag="LP859_002969"
     CDS             <1..>255
                     /locus_tag="LP859_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3030824.1"
                     /translation="ERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCG
                     KEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           90 a           49 c           46 g           70 t
ORIGIN      
        1 tgagcgactt tcaaaagcca ttatcgcctt gaaaccagaa ggccgtaagg cagttgatat
       61 tgaaaattcg attaatgaat ggcttcaatc cgtacccaaa aatcttttca aatcaattac
      121 ctttgattgt ggaaaagaat tctctaattg gaaaagtatt agtaatacga atgatattga
      181 tatttatttc gcagacccag gaacgccttc ccaacgggga ttaaatgaac attcaaacgg
      241 actcctccga aaaga
//
LOCUS       DAFEZB010000262          245 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFEZB010000262
VERSION     DAFEZB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.288"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT           83 a           32 c           65 g           65 t
ORIGIN      
        1 attggtaacg taaaaaattg gtcaatcgag taaatgccct tgtttggata gtggtgaaat
       61 gaatttgtgt aaataatatg cacagagtaa acaagaggga atttagaagt tagacaagtt
      121 agaatagcgc ctagattaat catgagcagc tactaaggga tctaggcttt gacgcaaact
      181 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
      241 gctgc
//
LOCUS       DAFEZB010000263          243 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFEZB010000263
VERSION     DAFEZB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.289"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..163
                     /locus_tag="LP859_002970"
     CDS             <1..163
                     /locus_tag="LP859_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBL3030825.1"
                     /translation="FGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSVEI
                     IEFVADKEE"
BASE COUNT           90 a           25 c           58 g           70 t
ORIGIN      
        1 attcggcttg ctacctgaag atgatgaagg taatgaatgg tttaaaatga cgcttacgga
       61 tgataatgga gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt
      121 cagcgttgaa atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat
      181 tttacagata aaggcttaga aggagtcacg acgaataata aaaaggtagt attaccttag
      241 gca
//
LOCUS       DAFEZB010000264          241 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFEZB010000264
VERSION     DAFEZB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.290"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(<1..220)
                     /locus_tag="LP859_002971"
     CDS             complement(<1..220)
                     /locus_tag="LP859_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3030826.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAFEZB010000265          233 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFEZB010000265
VERSION     DAFEZB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.291"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAFEZB010000266          232 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFEZB010000266
VERSION     DAFEZB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.292"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            complement(44..>232)
                     /locus_tag="LP859_002972"
     CDS             complement(44..>232)
                     /locus_tag="LP859_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3030827.1"
                     /translation="HQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRL
                     AKRILDNERGSDTSGEAA"
BASE COUNT           59 a           58 c           47 g           68 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tg
//
LOCUS       DAFEZB010000267          224 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFEZB010000267
VERSION     DAFEZB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.294"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>224
                     /locus_tag="LP859_002973"
     CDS             <1..>224
                     /locus_tag="LP859_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL3030828.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAFEZB010000268          220 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFEZB010000268
VERSION     DAFEZB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.295"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>220
                     /locus_tag="LP859_002974"
     CDS             <1..>220
                     /locus_tag="LP859_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030829.1"
                     /translation="GEVTYDKTKHQVKYAAKADTLKNRMKYNGETYELVINVKVNELA
                     NQNSVAKNQGTSIINKVEKDTNIITVYFP"
BASE COUNT           95 a           35 c           40 g           50 t
ORIGIN      
        1 ctggtgaagt gacctatgac aaaacaaaac accaggtaaa atatgcagcg aaagctgata
       61 cattaaaaaa tcgaatgaag tataacggcg aaacttacga attagtgatc aatgtaaaag
      121 tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca gggaacgtcg atcattaata
      181 aagtagaaaa ggatacaaat atcattaccg tttatttccc
//
LOCUS       DAFEZB010000269          218 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFEZB010000269
VERSION     DAFEZB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.296"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>218
                     /locus_tag="LP859_002975"
     CDS             <1..>218
                     /locus_tag="LP859_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3030830.1"
                     /translation="FIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSH
                     ASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           66 a           39 c           45 g           68 t
ORIGIN      
        1 ttttattgcg aaaaattgtc aggtatcttt gaccaccgtt attcgtacgt tgaaagagtt
       61 taaaagctac ttaccaaagc aatctaagag gattctcccc agagtattga tggttgatga
      121 atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag atggcgaaac
      181 aggacaatta attgatgtct tgcctacgcg taaattgc
//
LOCUS       DAFEZB010000270          211 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFEZB010000270
VERSION     DAFEZB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.298"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..164
                     /locus_tag="LP859_002976"
     CDS             <1..164
                     /locus_tag="LP859_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030831.1"
                     /translation="KGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYE
                     QQLSQKERV"
BASE COUNT           89 a           32 c           37 g           53 t
ORIGIN      
        1 taaaaggaaa aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc
       61 taggaaatcc attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg
      121 aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac
      181 tatctgaaaa acggatagtc ttttttcttt t
//
LOCUS       DAFEZB010000271          211 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFEZB010000271
VERSION     DAFEZB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.299"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>211
                     /locus_tag="LP859_002977"
     CDS             <1..>211
                     /locus_tag="LP859_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030832.1"
                     /translation="ESMDQMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRV
                     VTQRSLRQLNRTINAHEEKYHASKA"
BASE COUNT           86 a           40 c           38 g           47 t
ORIGIN      
        1 tagaaagtat ggatcagatg caatctattt acaatgaacg atcctttcga acgcttaaac
       61 agttaccaac gaatgaatac aaatatagtt acgaagaatt catgaagcgc aggaaacaat
      121 tccaacaaag agttgtcacg caacggagtt tgcgtcaatt aaatcgaacg attaatgccc
      181 atgaagaaaa ataccacgca agtaaagcct a
//
LOCUS       DAFEZB010000272          209 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFEZB010000272
VERSION     DAFEZB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.300"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>209
                     /locus_tag="LP859_002978"
     CDS             <1..>209
                     /locus_tag="LP859_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3030833.1"
                     /translation="RWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRT
                     PLPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT           51 a           43 c           44 g           71 t
ORIGIN      
        1 cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag
       61 aaaaatctat cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat
      121 ccttttcgga caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt
      181 gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DAFEZB010000273          207 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFEZB010000273
VERSION     DAFEZB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.301"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>207
                     /locus_tag="LP859_002979"
     CDS             <1..>207
                     /locus_tag="LP859_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3030834.1"
                     /translation="TRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTA
                     CNEAKTANVLDRTFTQEQPLEAIVT"
BASE COUNT           67 a           47 c           49 g           44 t
ORIGIN      
        1 acccgaaaat tgaagaagtg tttagcaaag cgtgggcttc aactcagtcg gcgccgaatc
       61 ggtcgaatca tgaaacgccg cggattgaca tctacctata cgatcgctca ttttaaaggg
      121 caacgaacag cttgtaatga agcgaaaaca gcgaatgtat tagatcggac ctttacacaa
      181 gaacagccat tggaagccat cgttacg
//
LOCUS       DAFEZB010000274          207 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFEZB010000274
VERSION     DAFEZB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.denovo.302"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..>207
                     /locus_tag="LP859_002980"
     CDS             <1..>207
                     /locus_tag="LP859_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3030835.1"
                     /translation="GSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLTYKEGSA
                     QVIDKSKGKDITSEGTLTYDSNSRT"
BASE COUNT           83 a           37 c           40 g           47 t
ORIGIN      
        1 ggatctgaag tacaatatct agtcacacaa aaatggcaca caaaaggcgt agacgctgcg
       61 tcagatcact acaaacaatt tagtattcaa gatccaattg aagctcgttt gacatataaa
      121 gaaggttctg cgcaagtaat agataagagt aaaggaaaag atattacttc tgaaggcaca
      181 ctaacctatg atagcaattc aagaacg
//
LOCUS       DAFEZB010000275         7506 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.104, whole genome shotgun sequence.
ACCESSION   DAFEZB010000275
VERSION     DAFEZB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.104"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..220
                     /locus_tag="LP859_002981"
     CDS             <1..220
                     /locus_tag="LP859_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL3030836.1"
                     /translation="LTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAAN
                     ATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            287..1024
                     /locus_tag="LP859_002982"
     CDS             287..1024
                     /locus_tag="LP859_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3030837.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1255..2523
                     /locus_tag="LP859_002983"
     CDS             1255..2523
                     /locus_tag="LP859_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL3030838.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3010..3474
                     /locus_tag="LP859_002984"
     CDS             3010..3474
                     /locus_tag="LP859_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3030839.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3458..4087
                     /locus_tag="LP859_002985"
     CDS             3458..4087
                     /locus_tag="LP859_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL3030840.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            4441..5919
                     /gene="msr(C)"
                     /locus_tag="LP859_002986"
     CDS             4441..5919
                     /gene="msr(C)"
                     /locus_tag="LP859_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL3030841.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            5966..6412
                     /locus_tag="LP859_002987"
     CDS             5966..6412
                     /locus_tag="LP859_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL3030842.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6611..7126)
                     /locus_tag="LP859_002988"
     CDS             complement(6611..7126)
                     /locus_tag="LP859_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3030843.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7243..>7506
                     /locus_tag="LP859_002989"
     CDS             7243..>7506
                     /locus_tag="LP859_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3030844.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKK"
BASE COUNT         2492 a         1316 c         1531 g         2167 t
ORIGIN      
        1 tctaaccgat ccaaaagaac gaagaggctt gtcagtaaag ccaatccaca tcaagaaaaa
       61 tgcttggatc ggagcaggag caactattct gccaggcgta acgattggcg agaatgccat
      121 tgttgcagcg aatgcaactg ttacgaaaga tgtcccagac aataccatcg ttgcaggaat
      181 ccctgccaaa atcgtcaaac ctgtcgaacg gattatttga acagtaagat gaaataagag
      241 atagtgagta taagcaagag atttttcaaa acaggaggag aatagaatga atgatttatc
      301 agaaaaagtg attgtcatca tgggcgcttc aagcggaatc ggtgaagcaa caacgaagct
      361 attagcagaa aaaggggcta aactagttat agctgcacgc cgagaagatc gcctaaaagc
      421 aataaaagaa tctttaccag aagctgagct atatattcaa acagcagatg taagagattt
      481 tgcacaagtc caagcagtta tcgatttagc gatggaaaag ttcggtcgaa tcgatgtatt
      541 gtacaataat gcaggaatca tgccaaccgc tccattagtt gaaggtcacc gtgatgaatg
      601 gcaaaatatg cttgatatca acatcatggg tgtgttaaat ggaatttccg ccgtacttcc
      661 aatcatggaa aaacaaaaat ctgggcatat catttcaact gattctgtgg cgggacatgt
      721 tgtttatcct gattccgccg tttattgcgg aacaaaattt gctgtccgtg caatcatgga
      781 aggattacgt caagagcaac ggcaaaataa catcaaatct acgatcatct ctccaggagc
      841 tgttcaaact gaattatatc aaaccatcag cgacaaaaag gctgctcttg aacttcatga
      901 agcgcaaaaa gaatggggat tgacctccga agatatagct tctgctgtag catttgcgat
      961 agaaacgcca gaccggatgt ctgtcagtaa catgattatc cgtccgacga cacaggaagt
     1021 ataatccata acggataata tccaaagaga attacttgtt aaatagttat tcaaacgacc
     1081 atcaatttaa ggcgtgaatg gagaagaata ttcttcattt acgtcttttt atgtagaaaa
     1141 agcaaattta tcaaaaagaa acaaatgtta tttaaatatg gacattgact tgcttttact
     1201 tttttagtta cactgttatt taggaaaagt ttcataattc aaaggagaaa aaagatgaaa
     1261 gcaaagaatt tattatttag cgttattctt ttaggaaatc tagcagttgc caacacaaca
     1321 gtgctagctg aagaatcgac agaaactgtc gcaactcaga catcgagttc ttcagaaaca
     1381 ggaattgaaa acgatacaga gagtactttg cagtcagcta ctgtctcaga aaatacaatt
     1441 tcaaccgaag aaccaacaac tgaaacaaca gaacaaacac aaaccgaaga cagtacatca
     1501 gcagaagctc agaacaatcc agagaatgca gaagaaatca cgaaccaatt gacagatggg
     1561 caaacattga cacataccca tcaagcagat ttgtctgaat tcgaaaacta tactggctat
     1621 gctttttatg ctcgctcatc tgctaattct caacaagcgt tcattgatag tattgcctca
     1681 actgcacaaa gtttggcaag tgccaatgac ttatatgcat cagtcatgat tgctcaagcc
     1741 atcgttgaaa gtggctgggg gaacagtact ttggcttctg cccctaatta caatctattt
     1801 gggattaaag gaagctataa tggccaatct gttacgatgc ctacttcgga atatgtgaat
     1861 gggcaatgga ttacggtgaa tgccgctttt agaaaatatc cttcgtacaa agaatcattg
     1921 caagataacg taacggtatt gaagacgact tcttttcagc caggcgttta ctactattcc
     1981 ggtgcatgga aaagcaatac gaactcttac aaagatgcta cggcttggct aacaggtcgt
     2041 tatgccactg cgcctaatta tggttctaca ttgaacaatg taatcgaaac ctataatctg
     2101 acacagtatg atacagcacc aacttcaact tctccgatgt atcgtctgta taaccgtcat
     2161 acaggagaac atttatatac attgaatgcc ggagaaaaaa attatctgcc aactgtaggc
     2221 tgggaatatg aagggattgc ttggcaagca ccaaacagcg gacaacctgt ctaccgttta
     2281 tacaatcctt acagtggaga ccatcattac acaatggcac aatctgagat caactcttta
     2341 acaaaaatcg gttggcgtta tgaaggtcta agtttttatt ctggtggttc caagccaatt
     2401 tatcgactat ttaatccaaa tgaaaaaact ggtacccatc actatacctt gagtgctaag
     2461 gaaagagatt ggctaacacc gatgggctgg agatatgaag gaataggatt ttatggttat
     2521 taaaaagagg tagtgaaaaa gctgccctgc atcaagtagt aagaacagcc aaacaaaaac
     2581 agattttcat gttttttgtt tggttgaact tattaccgca gatgcaagtt tttgaacacc
     2641 gtttactcaa aaaaggttgt gacaacatct tgtcataact tttttattac tgtttaagca
     2701 tactgatcca atttctaata ttcgacggga ttcttgcaaa aggcgatttc ggaaattttt
     2761 ggcggtgtaa aattttctac tgcagaagag ctatcttcta tagtttgata agaatcaata
     2821 atcttggttt tagcgttgtg ctttctttat cattttttcc gttcattcta caaaaaattt
     2881 cttttctcta tacttttcta ttgagagcgt tttttttatt acacagctga ttctttgact
     2941 tttttctttt tttattctat tattgacaag tcaatcgttg acgagtcaat tattagaaag
     3001 gaggaaatta tgtctttaga agagattagt caccttttat atcaaatcaa attagccgat
     3061 caaaaaatcg tccagatctt tgaagaaaat atcggtataa gtcttactag atacgagatc
     3121 atgatgagac tgaaagaaga aaatccattg ctacaaagca gtttgcaaga aaagttgcag
     3181 attgatcaag gagcagttac tcgtcatctg aaggtattgg aagcaaatgg ctatgtttcg
     3241 cgtcagagaa atcctcagaa caaccgggaa gtgtatgtag cgctaacaga aaaagcccag
     3301 aagaaaatcg agcattgtga aatggaccat aaagatacag gttccgtgct atccccggct
     3361 ttcagcgatg tagaaatgaa acaatttcta acacttttag ataaattgaa tacccaactt
     3421 tgcaatataa caacagaaac gaaagaggtt aataataatg gaacaaaaaa atgatttttc
     3481 aacaattatg actggtagaa gatccgttcg cgtatatgat gaaacataca aaatcccaca
     3541 cgaagaaatg ctggaaatga tcaaagaagc gacgactgca ccttcttctg tcaatatgca
     3601 accatggcgc tttgtggtcg ctgaaagcga ggaagcaaaa gaaatcttgc gtcctttgat
     3661 ccgtttcaat acgagacaaa atgatacttc ttctgcaatg gtcatgattt ttggcgatat
     3721 gcaatgttac gaatatggcg aagaaatcta taaccaagct tacgaatcag ggaagatgcc
     3781 aaaagaagtc agagatcaac aattagctgc gatcattcct tattacaaga gtttttcaag
     3841 agaagaaatg aacgatgtag tcaaagtcga ttctagctta gcggctatgc aattcatgtt
     3901 agttgcgcgt gatcatggct atgaaacaaa tccaattggt ggattcgaag cagatcagtt
     3961 ggcagaggca tttggtttgg ataaagaccg ttatgttcca gttattattt tatcaatagg
     4021 aaaagcggta gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga tcactacgtt
     4081 cgaataaaag gttttcagtt actattcagt agctattgct ttttttgaat cgtgtgtggt
     4141 aacctaataa aaaaggaggt cggaaaaatg actgcatcga tgaaattacg tttcgaactt
     4201 ttgaataaca actaaataaa ttcaaaagac atgtataaac aacaatttca tggatggagt
     4261 atgtccattt tttcgaacga cagtagaata ggtgaaagct atgacagatt tggtgattta
     4321 gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc agtttttttg caaacagatt
     4381 tttctgctct tttttagtat cgataattgt tgttttacat gaaataggag tgtaaaacat
     4441 atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa ccagctagaa
     4501 ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat aggagaaaat
     4561 ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc cgatcatggg
     4621 aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct cgctgaagca
     4681 aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga aacttcagag
     4741 cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct ttcttcttat
     4801 gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag tattgatcga
     4861 ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca tgatcgctat
     4921 tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat ccgagagttt
     4981 tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac tcagctacga
     5041 gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa
     5101 aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg
     5161 gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca aaagaatgcg
     5221 aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa
     5281 caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc catcatggga
     5341 caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga ttttcagttt
     5401 tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac aaccttatta
     5461 gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag ctttcaagta
     5521 tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt tgtcatgaga
     5581 caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg gtttgctcag
     5641 gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg tctgacgatt
     5701 gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac taattttatt
     5761 gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg aacgattttg
     5821 tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta tgaaataaaa
     5881 gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga tgattttcgt
     5941 gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa taatttttac
     6001 aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat ggctgcatca
     6061 agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg tggctttcat
     6121 tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa
     6181 tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt taggaatggt
     6241 gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc aattattctc
     6301 tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc agttgatcat
     6361 cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat gatggaacac
     6421 tgttatttga aaaaaggcct tggcatgctt tttgtcatag tctcttgaat gcagttctag
     6481 aaaaatagtg atccgaacga taggttttgg cctgtcgttc ggatcgcttt ttcttttcca
     6541 actgtattgc tcttttattg aagataattt gctcttcaat agatatttct agaaaggttc
     6601 tgtttatccc ttactcaaaa aactgctgca tttgttctct tacttgctga caccattgga
     6661 gttcctgctg acaaagcata attctgcgct ggatcacaac ataacgtcca aattcttgtt
     6721 tcttgtctag cgagaggtca ttcaattttt tttgattttt ttgcatgatt ttgttcagct
     6781 gttgttctct agtgaatagc agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat
     6841 agatttttgc tagaaattca tctttttgcg taggagcggg agtttcctct ttgatccatt
     6901 cctctacaag agcacgtcct tcatcagtca agtggtagat ctttttttga gagtgctcgt
     6961 ctttgtccaa aacggtcaca taaccctgct ggtctaactt ggcaagcgtt gtatagatct
     7021 gactgtgatg ggcttcccaa aaaattgtga tcagctgctt caattcatac ccagaacgtg
     7081 gtttggataa aagcacgcat aataatatat aggctaaagt attcataaaa atcctacctc
     7141 tcgtcttatt ttctttatta tatcacagta aagtttccac gttttacatg tgcatattta
     7201 catgtaaaat aaatcatgta tgatgctaat agagagaaag ggatggaaat attggagaat
     7261 caaatagata tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt
     7321 gtagcagcta cctgtatgat cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa
     7381 acgattgctg cacagttaaa cgctacgcca gctgaaacga ccctgttatt tactagttac
     7441 atgttagtca ctggtgtggt gatgctattt acaggtttct tatctagtcg gatcggtttg
     7501 aaaaaa
//
LOCUS       DAFEZB010000276         6419 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.109, whole genome shotgun sequence.
ACCESSION   DAFEZB010000276
VERSION     DAFEZB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.109"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            213..2660
                     /locus_tag="LP859_002990"
     CDS             213..2660
                     /locus_tag="LP859_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL3030845.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2890..3957
                     /gene="rlmN"
                     /locus_tag="LP859_002991"
     CDS             2890..3957
                     /gene="rlmN"
                     /locus_tag="LP859_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL3030846.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4150..5253)
                     /locus_tag="LP859_002992"
     CDS             complement(4150..5253)
                     /locus_tag="LP859_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3030847.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5806..6354
                     /gene="aac(6')"
                     /locus_tag="LP859_002993"
     CDS             5806..6354
                     /gene="aac(6')"
                     /locus_tag="LP859_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL3030848.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2151 a         1099 c         1377 g         1792 t
ORIGIN      
        1 gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat
       61 ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga
      121 aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt
      181 ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa
      241 aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga
      301 ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt
      361 taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct
      421 gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg
      481 gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac
      541 atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg
      601 atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga
      661 ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga agttatttag
      721 cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc
      781 tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg
      841 ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga
      901 aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga
      961 agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa
     1021 atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc
     1081 ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt
     1141 tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac
     1201 aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg
     1261 atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc
     1321 aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat
     1381 tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata
     1441 tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac
     1501 tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg
     1561 aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg
     1621 gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg
     1681 gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc
     1741 cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg
     1801 gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac
     1861 aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa
     1921 atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc
     1981 caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca
     2041 gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc
     2101 atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa
     2161 atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg
     2221 attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg
     2281 agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg
     2341 ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg
     2401 aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag
     2461 cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg
     2521 gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt
     2581 cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc
     2641 cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa
     2701 aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg
     2761 gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga
     2821 agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg
     2881 gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg
     2941 tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa
     3001 aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta
     3061 tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt
     3121 acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga
     3181 caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg
     3241 ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca
     3301 cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat
     3361 gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg
     3421 catatcatca atgatgccaa aggattggca attggtgctc ggcacattac agtttccact
     3481 agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct
     3541 atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt
     3601 ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga
     3661 atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa
     3721 ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat
     3781 ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat
     3841 gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt
     3901 gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg
     3961 tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt
     4021 caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa
     4081 aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt
     4141 agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt
     4201 ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt
     4261 aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag
     4321 attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc
     4381 aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc
     4441 tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc
     4501 catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat
     4561 atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc
     4621 ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct
     4681 atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa
     4741 agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc
     4801 aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc
     4861 gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt
     4921 gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc
     4981 accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat
     5041 atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt
     5101 caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac
     5161 tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat
     5221 ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa
     5281 cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa
     5341 cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta
     5401 tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct
     5461 tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag
     5521 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
     5581 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
     5641 ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag taagataaat
     5701 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
     5761 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
     5821 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
     5881 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
     5941 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
     6001 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
     6061 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
     6121 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
     6181 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
     6241 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
     6301 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
     6361 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAFEZB010000277         4762 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.126, whole genome shotgun sequence.
ACCESSION   DAFEZB010000277
VERSION     DAFEZB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.126"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..331
                     /locus_tag="LP859_002994"
     CDS             <1..331
                     /locus_tag="LP859_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030849.1"
                     /translation="AGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
     gene            429..1802
                     /gene="tet(L)"
                     /locus_tag="LP859_002995"
     CDS             429..1802
                     /gene="tet(L)"
                     /locus_tag="LP859_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL3030850.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2366..3628
                     /locus_tag="LP859_002996"
     CDS             2366..3628
                     /locus_tag="LP859_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL3030851.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3873..4760
                     /locus_tag="LP859_002997"
     CDS             3873..4760
                     /locus_tag="LP859_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL3030852.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         1653 a          650 c          972 g         1487 t
ORIGIN      
        1 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
       61 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
      121 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
      181 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
      241 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
      301 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
      361 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
      421 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
      481 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
      541 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
      601 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
      661 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
      721 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
      781 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
      841 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
      901 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
      961 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
     1021 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
     1081 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
     1141 atatcaggaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
     1201 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
     1261 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
     1321 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
     1381 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
     1441 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
     1501 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
     1561 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
     1621 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
     1681 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
     1741 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
     1801 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
     1861 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
     1921 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
     1981 ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata
     2041 aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc
     2101 tgtcccttgc tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag
     2161 agggcaggtt tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat
     2221 ggttttggtc ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata
     2281 gtgtgttata ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg
     2341 ccacctaaaa aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa
     2401 atcagctgga ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa
     2461 tgatgatatt gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat
     2521 tgattacaac gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac
     2581 gaggaaagat gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga
     2641 gcaactggat ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga
     2701 acgatatggc aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca
     2761 catgcattta ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa
     2821 tcgtcaagaa ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt
     2881 tgagttgaag cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa
     2941 aaaacaaact ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga
     3001 tgaatggact gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca
     3061 catgaaaagt gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat
     3121 aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa
     3181 cttagtgact gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag
     3241 tactgatatg gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa
     3301 atatagtggt cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga
     3361 agaaaacaag tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta
     3421 tgaaagcact aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt
     3481 ttttaagggg tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga
     3541 tttagaacct aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg
     3601 aagtcgagat cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt
     3661 ttgatggttt tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta
     3721 ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa
     3781 cccttaaaat tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa
     3841 taactaaata gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag
     3901 atgaaaaatc aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag
     3961 aaaagaaaat cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg
     4021 ctgaaagagt aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg
     4081 aaagaaagtt gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga
     4141 ggagagcaat gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa
     4201 agccaacagt tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag
     4261 aattaaataa gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa
     4321 aaattaataa aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag
     4381 ataattctta taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga
     4441 atacagaaaa ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat
     4501 tagactatga tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg
     4561 atatacaatc ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga
     4621 ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta
     4681 aaaggttaat ctcctatggt ggtttgttaa aagaaataca taaaaaatgg ttctgttgca
     4741 aagttttaaa taaagaataa aa
//
LOCUS       DAFEZB010000278         4242 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.132, whole genome shotgun sequence.
ACCESSION   DAFEZB010000278
VERSION     DAFEZB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.132"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_002998"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL3030853.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="LP859_002999"
     CDS             2039..2227
                     /locus_tag="LP859_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL3030854.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="LP859_003000"
     CDS             complement(2285..2638)
                     /locus_tag="LP859_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030855.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="LP859_003001"
     CDS             3169..3591
                     /locus_tag="LP859_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3030856.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="LP859_003002"
     CDS             3588..3818
                     /locus_tag="LP859_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030857.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(4012..4235)
                     /locus_tag="LP859_003003"
                     /pseudo
     CDS             complement(4012..4235)
                     /locus_tag="LP859_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364935.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
BASE COUNT         1417 a          699 c          816 g         1310 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat ta
//
LOCUS       DAFEZB010000279         4242 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.133, whole genome shotgun sequence.
ACCESSION   DAFEZB010000279
VERSION     DAFEZB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.133"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_003004"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL3030858.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="LP859_003005"
     CDS             2039..2227
                     /locus_tag="LP859_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL3030859.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="LP859_003006"
     CDS             complement(2285..2638)
                     /locus_tag="LP859_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030860.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="LP859_003007"
     CDS             3169..3591
                     /locus_tag="LP859_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3030861.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="LP859_003008"
     CDS             3588..3818
                     /locus_tag="LP859_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030862.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(4012..4235)
                     /locus_tag="LP859_003009"
                     /pseudo
     CDS             complement(4012..4235)
                     /locus_tag="LP859_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364935.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
BASE COUNT         1416 a          699 c          817 g         1310 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat ta
//
LOCUS       DAFEZB010000280         4189 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.134, whole genome shotgun sequence.
ACCESSION   DAFEZB010000280
VERSION     DAFEZB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.134"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            26..601
                     /locus_tag="LP859_003010"
     CDS             26..601
                     /locus_tag="LP859_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL3030863.1"
                     /translation="MIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQ
                     QIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIIT
                     GEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPI
                     GLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            859..2361
                     /gene="eat(A)"
                     /locus_tag="LP859_003011"
     CDS             859..2361
                     /gene="eat(A)"
                     /locus_tag="LP859_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL3030864.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2389..2955
                     /locus_tag="LP859_003012"
     CDS             2389..2955
                     /locus_tag="LP859_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3030865.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3166..>4189
                     /locus_tag="LP859_003013"
     CDS             3166..>4189
                     /locus_tag="LP859_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL3030866.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKA"
BASE COUNT         1439 a          720 c          921 g         1109 t
ORIGIN      
        1 aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac
       61 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct
      121 agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt
      181 ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt
      241 gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa
      301 aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat
      361 ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
      421 cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt
      481 cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt
      541 agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta
      601 gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
      661 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
      721 acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt
      781 ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt
      841 tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac
      901 agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta
      961 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
     1021 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
     1081 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
     1141 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
     1201 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
     1261 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
     1321 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
     1381 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
     1441 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
     1501 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
     1561 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
     1621 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
     1681 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
     1741 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
     1801 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
     1861 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     1921 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     1981 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     2041 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     2101 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     2161 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     2221 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     2281 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     2341 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     2401 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     2461 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     2521 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     2581 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     2641 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     2701 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     2761 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     2821 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     2881 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     2941 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     3001 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     3061 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     3121 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     3181 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     3241 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     3301 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     3361 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     3421 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     3481 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     3541 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     3601 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     3661 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     3721 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     3781 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     3841 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     3901 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     3961 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     4021 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     4081 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     4141 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctg
//
LOCUS       DAFEZB010000281         3701 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.145, whole genome shotgun sequence.
ACCESSION   DAFEZB010000281
VERSION     DAFEZB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.145"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            109..192
                     /locus_tag="LP859_003014"
     CDS             109..192
                     /locus_tag="LP859_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL3030867.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="LP859_003015"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="LP859_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL3030868.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="LP859_003016"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="LP859_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1647..2312
                     /locus_tag="LP859_003017"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="LP859_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="LP859_003018"
     CDS             2348..2593
                     /locus_tag="LP859_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3030869.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="LP859_003019"
     CDS             2696..3565
                     /locus_tag="LP859_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL3030870.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..>3701
                     /locus_tag="LP859_003020"
     CDS             3546..>3701
                     /locus_tag="LP859_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3030871.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCG"
BASE COUNT         1332 a          547 c          697 g         1125 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg c
//
LOCUS       DAFEZB010000282         3533 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.146, whole genome shotgun sequence.
ACCESSION   DAFEZB010000282
VERSION     DAFEZB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.146"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..584
                     /locus_tag="LP859_003021"
     CDS             <1..584
                     /locus_tag="LP859_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL3030872.1"
                     /translation="LLWFGWFGFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAK
                     VKGNGFDFVDVLTGSVAGLAAITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWD
                     DALDVWGVHGMGGFTGTILIGIFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTT
                     VILKSAMHFTTITTTKEEQEEGLDFFFFGNKLN"
     gene            615..833
                     /locus_tag="LP859_003022"
                     /pseudo
     CDS             615..833
                     /locus_tag="LP859_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1252..1725
                     /gene="dfrF"
                     /locus_tag="LP859_003023"
     CDS             1252..1725
                     /gene="dfrF"
                     /locus_tag="LP859_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL3030873.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1852..2508
                     /locus_tag="LP859_003024"
     CDS             1852..2508
                     /locus_tag="LP859_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL3030874.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            2690..3220
                     /locus_tag="LP859_003025"
     CDS             2690..3220
                     /locus_tag="LP859_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3030875.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1214 a          503 c          757 g         1059 t
ORIGIN      
        1 gactgctttg gtttggctgg ttcggtttta attccggtgg tgcacttcga gcagatgaac
       61 aagcagtcaa tgcatttggt tcaacattca tcgctcttgc atttgcgatg attacttggc
      121 tgatcattgc aaaagtaaaa ggaaatggct ttgattttgt cgacgtacta acaggatccg
      181 ttgctggatt ggcagccatt acgccttgtg ccggttatgt agaagcaaaa agtgccatgc
      241 tgattgggat cattgcgggt atcgtctgtc atgctgctgt tgattttcgt aaaaagaaac
      301 aatgggatga tgcacttgat gtgtggggtg ttcatggcat gggcggattc acaggtacga
      361 ttctgatagg gatctttgca tctaacagcc atttactaac gaatccaagc agctggtact
      421 tcttagggat tcaagtgatt ggtgtcatca ttactgccgt gtatgcctat gtgctgacaa
      481 cagtgatttt aaaatcggcg atgcacttta caacgatcac tactacaaaa gaagaacaag
      541 aagaaggatt ggactttttc ttctttggca acaaactcaa ttgaaacaaa ttcaaataga
      601 aaagaggaag attcatgcaa caaagcaaaa cgaaaattcc tgcaaaaaaa ttaacgttct
      661 ttggcttttt ggctatgacc atctcaatgg tcgtttcatt atatgaatat cccacattcg
      721 cgacctcagg cttcagctta gtatttttct tgttattagg cggtttgcta tggtttatcc
      781 ctgtggcatt gtgcgcagca gagatggcaa cggtgaaagg atgggaaaaa ggtgtttccc
      841 aaacaaaaag atattaagaa acagcccatg tttacctgct tgcaaacaaa agaatattgc
      901 tactagacta agagatacta cataagtgtg ttggtatctc ttttttgttg ccgtgaaaca
      961 gcagactatt tcatttctgc ttgattacct taaaacttat tagtctatgg tttgtcaagg
     1021 gcttgttgcg tgagaaatgc tgaatatcac cctttacaaa acataggctg ataagtaact
     1081 tttcaaaagc agaaaagaaa tggcaatgct tataacaata tgatataatg cagatgaaga
     1141 caaaaaagga atttgtcgag ctggttagtt cgagataatt ttgagtttgt taagatgtgg
     1201 tgcacttgat ggaatcacag gtacgacatc atattatgga ggatgaaata gatgataggt
     1261 ttgattgttg cgaggtcaaa gaataatgtt ataggcaaga atggtaatat accatggaaa
     1321 ataaagggag aacaaaagca atttagagag ttaacaacgg gtaatgtggt tattatgggg
     1381 cgaaagtctt atgaagaaat cggtcatccg ttgcctaata gaatgaatat tgttgtttcc
     1441 accacaacag agtatcaagg agataattta gtttcagtta aatcattaga agatgcatta
     1501 ttattggcta aaggacgaga tgtatacata tctggtggat atggactatt taaggaagct
     1561 ttgcaaatag tagataaaat gtatatcaca gaagtagatt taaatattga agatggagat
     1621 acattctttc cagaatttga tatcaatgat tttgaagttt tgatagggga aacacttggt
     1681 gaggaagtga aatatacgag aacattttat gtaaggaaaa aatgaattga gtaaattttg
     1741 gatttagggt gttttcataa atatattgct ttttatgcat atataaattg ttgtaagaca
     1801 aattccagtt tgtatggggc gataaaatca gaatttgaga gaggattgaa tatgaataag
     1861 gaatggtctg aacttaataa aacaatgcag gcacaaataa aaaagaaaga tacttataag
     1921 aggggtattg atactttact tactttacga agtcagttaa tacaaacctt agtatctttc
     1981 aaagaagaat tatgcagaga agattttaac tcaatcccct tcataaacgc tgatggttat
     2041 catagtaaga cgattgctta ttctatttgg catattttcc gcattgaaga tatagttgtg
     2101 catacagtga taaatgaaga tgaacaagta ttttttgcag gcaattatca agagcgtatc
     2161 aattcaccta tcattacaac aggaaatgaa ctcatgaaac agcagattgc agacttttca
     2221 aaacagctta atctggaaga attatattta tatattttcg aagtatggga aagcactgag
     2281 aaaatgcttg aacggttatc ttatgatgaa ttgaaaagaa aaataccgaa agaaagaaaa
     2341 ggatacttag aatcgttgaa tgtagtaaac gacaatgaaa aagcaatttg gctgattgat
     2401 tattggtgta ataaagatat ttgtggacta attcaaatgc cgttttcaag acattggatt
     2461 atgcacacag aagcctgttt gcgtataaag aacaagatac attcataggc aaaaatttca
     2521 gtttgtagaa ctaaggaaat tggaatttaa ggaggaataa agtatggtta gtgattttat
     2581 aagagcagca tttccatgga ttttaatggg tttatttgta gcaatcagtt gttcccttat
     2641 gagtaaaaaa gagaaataaa ttcctgtttg tatttataaa agagaaaaaa tgagaaaagt
     2701 agttttattt attgccatga gccttgacgg atatattgcg gacggtaatg gtggagtagc
     2761 ttggctaaat ggtcatggaa atgacaatga aaacatcgac acttatacag aatttaccaa
     2821 agatatagat actgtcttaa tggggtggaa tacttctcat caggttgtta ctgaactttc
     2881 tccgcaagaa tgggtatata ataaatttac aacttatgta ttaacacata aagagtgcaa
     2941 ttcttcccaa caaattcatt ttacaagtga aaatcctgtt ttattattgg aacggctaaa
     3001 acaagaagca ggaaaggata tttggatttg tggcggagca aatcttgttc agcagttggt
     3061 aagcaaaaat atgattgata aatattacat ttctgttatt cctactctgt taggaaatgg
     3121 ggttcgtctt ttgggcaaca tagacaggga aataaagtta aggctttgta aaacacagac
     3181 ttataacgga ataaccgatt tgatttatat gcgtagataa ctagagagaa acagaaaagc
     3241 tgttaaaccg acggagcgat ccgaccacgt tggcgggaaa atccttaaga tttttccgcc
     3301 atgcttgcaa gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc gttcaaagcc
     3361 cccgttatct aacaaggggg gcacaaaaaa caagaaacac tatacactaa caagcggaaa
     3421 acgctgtatt tataaggcaa aaccccattt ttacagtagt gatataaatt gtacctatat
     3481 cactatgaaa attgaatagt aaatatgaga aagacagttg atttcaaaaa ata
//
LOCUS       DAFEZB010000283         3533 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.147, whole genome shotgun sequence.
ACCESSION   DAFEZB010000283
VERSION     DAFEZB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.147"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            192..1232
                     /locus_tag="LP859_003026"
     CDS             192..1232
                     /locus_tag="LP859_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL3030876.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1330..1518
                     /locus_tag="LP859_003027"
     CDS             1330..1518
                     /locus_tag="LP859_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL3030877.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1576..1929)
                     /locus_tag="LP859_003028"
     CDS             complement(1576..1929)
                     /locus_tag="LP859_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030878.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2460..2882
                     /locus_tag="LP859_003029"
     CDS             2460..2882
                     /locus_tag="LP859_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3030879.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2879..3109
                     /locus_tag="LP859_003030"
     CDS             2879..3109
                     /locus_tag="LP859_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3030880.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(3303..3526)
                     /locus_tag="LP859_003031"
                     /pseudo
     CDS             complement(3303..3526)
                     /locus_tag="LP859_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364935.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
BASE COUNT         1161 a          582 c          655 g         1135 t
ORIGIN      
        1 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
       61 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
      121 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
      181 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
      241 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
      301 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
      361 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
      421 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
      481 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
      541 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
      601 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
      661 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
      721 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
      781 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
      841 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
      901 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
      961 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
     1021 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
     1081 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
     1141 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
     1201 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
     1261 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
     1321 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
     1381 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
     1441 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
     1501 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
     1561 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
     1621 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
     1681 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
     1741 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
     1801 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
     1861 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
     1921 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
     1981 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
     2041 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
     2101 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
     2161 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
     2221 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
     2281 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
     2341 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
     2401 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
     2461 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
     2521 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
     2581 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
     2641 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
     2701 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
     2761 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
     2821 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
     2881 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
     2941 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
     3001 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
     3061 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
     3121 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
     3181 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
     3241 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
     3301 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
     3361 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
     3421 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
     3481 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta tta
//
LOCUS       DAFEZB010000284         3249 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.150, whole genome shotgun sequence.
ACCESSION   DAFEZB010000284
VERSION     DAFEZB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.150"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            39..536
                     /gene="dfrG"
                     /locus_tag="LP859_003032"
     CDS             39..536
                     /gene="dfrG"
                     /locus_tag="LP859_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBL3030881.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(608..2560)
                     /locus_tag="LP859_003033"
     CDS             complement(608..2560)
                     /locus_tag="LP859_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBL3030882.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2567..2803)
                     /locus_tag="LP859_003034"
     CDS             complement(2567..2803)
                     /locus_tag="LP859_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3030883.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3038..>3249)
                     /locus_tag="LP859_003035"
     CDS             complement(3038..>3249)
                     /locus_tag="LP859_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL3030884.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT          981 a          580 c          534 g         1154 t
ORIGIN      
        1 aattagatgg taaattagtt aaaaaggctg tgttataaat gaaagtttct ttgattgctg
       61 cgatggataa gaatagagtg attggcaaag agaatgacat tccttggagg attcccaagg
      121 actgggaata tgttaaaaat actacaaagg gacatccgat aatattaggt aggaagaacc
      181 ttgaatcaat cggaagagcc ttacctgaca gaagaaatat tattctgacg agagataagg
      241 ggtttacctt taatggttgt gaaattgttc attcaataga agatgttttt gagttatgta
      301 aaaacgaaga agaaattttt attttcggag gagaacagat ttataatttg tttttccctt
      361 atgttgagaa aatgtacatc acaaaaatac atcatgaatt cgaaggagat actttttttc
      421 cagaagtgaa ttatgaggaa tggaatgagg tatttgccca aaaagggata aagaatgata
      481 aaaatccgta taactactat tttcatgtat atgaaagaaa aaacttattg agttaacggg
      541 tgcgttaatt gaagaagaac agtaaagata aagcacaaga gcttgggatt actccaagct
      601 ctttttgtca tttgaaataa attatatctt tccaatcaaa ttgtttatct gtaatcccta
      661 atctttgagc aggtgtcaaa acctttttat ccgctgattt aaaagggcga caaaagttgt
      721 agtaagttct tagaattgta atcgcaaatt gagcatactt cggattgaag ttagcgtaaa
      781 tatagctctt cttatcacca cgagctgttg ttaatggtct ttcaagagat gatatataac
      841 gtcttatctg ttgaataaag ctattggtcg aatggtcatt aacatttaat agcatctttg
      901 cgatttcgtt tggttccaga gcagataaat ccgttctaca atcaactgaa taaaatcctt
      961 tatccttagt ggctaatgga tgtttgatag ggttatcggc atatttgcga tatctttctc
     1021 catctttact aatagcctct tcgtggaatt gatgacttgt tagtaattca gttagataat
     1081 gagatgccaa agtccgtaga aactttgttt tgaatcctct gatatctccc caatcgagca
     1141 gttctgcttt tgcttgtccg aattctttta agcattgttt tctgcttttc gtcttgttaa
     1201 cttggcaaat aaaatgatgt gcatcgctca accttaattc tttcgtgaat actctataaa
     1261 aggcgtttct aatagagaag tcatcatcac taatcattcg ccattcgtcg gcattaacca
     1321 tttgtttaat taaccaatag tgagcagttg tagtataagg agcatcaaca tttaaaccat
     1381 cgacaaatct actcctatta gtaattttcc ctaattcgtg aagataagca ttcctatttt
     1441 cgctatcgtt agcggatggt tcttgtggat aataagacca atctaaccta tcatttttac
     1501 gactaaaagt attgagatgg tcttctttga attttcttgt atcttcattt aattcatcca
     1561 ttgaaatatt ccaatcatag gcaacatcag aacggaacac atatcttgat aagacctcag
     1621 cagatacaac tatatacgtt tgcatattaa ggtcttcaaa ccccgtatat ttctttgaac
     1681 cttgtccttt cttacggacg ttatttaggt agtagtgcat cttgtccgta ttaagccaca
     1741 tctcgttaaa tttctttgtt tgaaggggtt gtgtctcata tcgttctaaa aactccaaac
     1801 aacgcctata cagccactcc agcttatgat agtaggttcc aactccaatt cctaaaatat
     1861 cacaagtacg actgactgaa acccttccaa caaccatctt agcgaacatt ggaagaatgg
     1921 tgttcttttg ttggtgataa gtagtggttt cttctctttt tggtagaaca ttagtgaact
     1981 tcttacacgc cttacattga tagcgttgag atttgcccct acttttcccc cttttgtaaa
     2041 attgcttcgg ctcattgaat ggggttgatt cttcttccga gcagccttct ttatgaaatt
     2101 gataatctgg ctcaacatct tggatgctat tgatttctat aaggcgttta atttcttcaa
     2161 caacagacca attagataaa ggggttactg tacagttttg cgatacacct ctgccaatag
     2221 gattgtcatt gcagtacata cctttatgac ctttatcttt ggaactacca gtcattttat
     2281 atcgacttgg tttccctttt acatctttga atttgtgctg ttctttccca aaattacaac
     2341 aaaacggatt gatgcagtaa ttatattgaa tgggatattc agaaccgttc catttaaaag
     2401 acgttggcaa gaacaacatt ttgttatacc ttgttgcaaa tcgttttgga gttaataaat
     2461 catattgttt agccctatct tttaattctt cgtttgaaat cggaatttta acatctacaa
     2521 tttcatcttc cttcgtggct aatcttttaa gcttggtcat tgtctatcaa cccactctat
     2581 ctttgatacc cattttttca acaatccgct tattacttcg cttgttggca atccattcaa
     2641 taaacttctt atcgtctaaa atgttcaata agaattcatc cactgcttcg ctttcagtac
     2701 gctctgcata ttcagcgtat tgcttaacta tcacccttac ttgctctgat atttcccaat
     2761 ccacagactt agcgttctta ttttttggct caagaaattg cataggaaaa tgcacccctt
     2821 tttgcttagt aaattgtaac cattttaaca caccaatttg cattcctaca ataattattt
     2881 cacgttcgca tttttatata cattttaact tacattttgt ggtgtaaatt aacaacaaac
     2941 gttgatttaa caataaaaaa cgagtatcaa attcagttgc aatttgatac tcatttaggt
     3001 ttacattttc actttttgta ctccaaaact gaactactta atatgcaaga cgttgtcttt
     3061 tttttgtata ttcaattttg aaaacatttc cgcaaagttc agacggacct cgatgtgttg
     3121 atgaataaaa tttattagta ataacttcta taaggttatc aatccctata ttactttttg
     3181 cacttccatg ataaagaggg aacagagaac aattctgaaa tcttatgctt tcctcttgtt
     3241 cgagttcca
//
LOCUS       DAFEZB010000285         3049 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.156, whole genome shotgun sequence.
ACCESSION   DAFEZB010000285
VERSION     DAFEZB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.156"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_003036"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL3030885.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3049
                     /gene="tet"
                     /locus_tag="LP859_003037"
     CDS             2039..>3049
                     /gene="tet"
                     /locus_tag="LP859_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HBL3030886.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFL"
BASE COUNT          984 a          464 c          629 g          972 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agcttttta
//
LOCUS       DAFEZB010000286         3049 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.157, whole genome shotgun sequence.
ACCESSION   DAFEZB010000286
VERSION     DAFEZB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.157"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_003038"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LP859_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL3030887.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3049
                     /gene="tet"
                     /locus_tag="LP859_003039"
     CDS             2039..>3049
                     /gene="tet"
                     /locus_tag="LP859_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HBL3030888.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFL"
BASE COUNT          983 a          464 c          630 g          972 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agcttttta
//
LOCUS       DAFEZB010000287         2599 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.163, whole genome shotgun sequence.
ACCESSION   DAFEZB010000287
VERSION     DAFEZB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.163"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..600
                     /locus_tag="LP859_003040"
     CDS             <1..600
                     /locus_tag="LP859_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3030889.1"
                     /translation="VLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQ
                     IEYECCAIEDVDFPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPV
                     FTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNS
                     FIINQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            633..1552
                     /gene="ant(6)"
                     /locus_tag="LP859_003041"
                     /pseudo
     CDS             633..1552
                     /gene="ant(6)"
                     /locus_tag="LP859_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            1549..2091
                     /gene="sat4"
                     /locus_tag="LP859_003042"
     CDS             1549..2091
                     /gene="sat4"
                     /locus_tag="LP859_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3030890.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2184..>2599
                     /locus_tag="LP859_003043"
     CDS             2184..>2599
                     /locus_tag="LP859_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBL3030891.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR"
BASE COUNT          898 a          395 c          599 g          707 t
ORIGIN      
        1 gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct
       61 tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc
      121 cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag
      181 agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta
      241 ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat
      301 cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg
      361 catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa
      421 aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt
      481 tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg
      541 atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa
      601 taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt
      661 tagtactttc tttagcagaa caggatgaac gaacgtattc gaattgtgac ccttgagggg
      721 tcacgcgcaa atattaatat acctaaagat gaatttcagg attatgatat tacatatttt
      781 gtaagtgata tagaacctag aaccgtttat atctaatgat gactggctta atcaatttgg
      841 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
      901 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
      961 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     1021 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     1081 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     1141 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     1201 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     1261 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     1321 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     1381 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     1441 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     1501 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     1561 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     1621 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
     1681 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     1741 gaatatattg acaatactga taagataata tatctttact accaagacga taaatgcgtc
     1801 ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta
     1861 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
     1921 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
     1981 tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
     2041 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
     2101 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
     2161 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     2221 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     2281 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     2341 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     2401 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     2461 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     2521 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     2581 cgaatagctt agacagccg
//
LOCUS       DAFEZB010000288         2595 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.164, whole genome shotgun sequence.
ACCESSION   DAFEZB010000288
VERSION     DAFEZB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.164"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..600
                     /locus_tag="LP859_003044"
     CDS             <1..600
                     /locus_tag="LP859_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3030892.1"
                     /translation="VLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQ
                     IEYECCAIEDVDFPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPV
                     FTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNS
                     FIINQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            633..1548
                     /gene="ant(6)"
                     /locus_tag="LP859_003045"
                     /pseudo
     CDS             633..1548
                     /gene="ant(6)"
                     /locus_tag="LP859_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            1545..2087
                     /gene="sat4"
                     /locus_tag="LP859_003046"
     CDS             1545..2087
                     /gene="sat4"
                     /locus_tag="LP859_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3030893.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2180..>2595
                     /locus_tag="LP859_003047"
     CDS             2180..>2595
                     /locus_tag="LP859_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBL3030894.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR"
BASE COUNT          896 a          394 c          598 g          707 t
ORIGIN      
        1 gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct
       61 tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc
      121 cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag
      181 agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta
      241 ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat
      301 cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg
      361 catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa
      421 aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt
      481 tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg
      541 atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa
      601 taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt
      661 tagtactttc tttagcagaa caggatgaac gtattcgaat tgtgaccctt gaggggtcac
      721 gcgcaaatat taatatacct aaagatgaat ttcaggatta tgatattaca tattttgtaa
      781 gtgatataga acctagaacc gtttatatct aatgatgact ggcttaatca atttgggaat
      841 ataataatga tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt
      901 tcctatctta tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa
      961 gagttagata attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt
     1021 agaattaaaa gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca
     1081 agggagtatg atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga
     1141 ttgtgccgta aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag
     1201 ctgctgagaa tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc
     1261 aagaactata agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc
     1321 acctaccgga tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg
     1381 ttcagggcgg tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat
     1441 aggaatataa caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc
     1501 ctggatagca catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga
     1561 aagcagggca cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga
     1621 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     1681 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     1741 atattgacaa tactgataag ataatatatc tttactacca agacgataaa tgcgtcggaa
     1801 aagttaaact gcgaaaaaat tggaaccggt acgcttatat agaagatatc gccgtatgta
     1861 aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa
     1921 agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta
     1981 aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
     2041 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
     2101 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
     2161 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     2221 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     2281 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     2341 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     2401 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     2461 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     2521 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     2581 tagcttagac agccg
//
LOCUS       DAFEZB010000289         2592 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.165, whole genome shotgun sequence.
ACCESSION   DAFEZB010000289
VERSION     DAFEZB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.165"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..600
                     /locus_tag="LP859_003048"
     CDS             <1..600
                     /locus_tag="LP859_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3030895.1"
                     /translation="VLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQ
                     IEYECCAIEDVDFPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPV
                     FTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNS
                     FIINQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            633..1545
                     /gene="ant(6)"
                     /locus_tag="LP859_003049"
                     /pseudo
     CDS             633..1545
                     /gene="ant(6)"
                     /locus_tag="LP859_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            1542..2084
                     /gene="sat4"
                     /locus_tag="LP859_003050"
     CDS             1542..2084
                     /gene="sat4"
                     /locus_tag="LP859_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3030896.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2177..>2592
                     /locus_tag="LP859_003051"
     CDS             2177..>2592
                     /locus_tag="LP859_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBL3030897.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR"
BASE COUNT          895 a          393 c          598 g          706 t
ORIGIN      
        1 gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct
       61 tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc
      121 cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag
      181 agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta
      241 ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat
      301 cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg
      361 catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa
      421 aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt
      481 tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg
      541 atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa
      601 taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt
      661 tagtactttc tttagcagaa caggatgaac gaacgtattc gaattgtgac ccttgagggg
      721 tcacgcgcaa atattaatat acctaaagat gaatttcagg attatgatat tacatatttt
      781 gtaagtgata tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata
      841 ataatgatgc aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc
      901 tatcttatgc tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag
      961 ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga
     1021 attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg
     1081 gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg
     1141 tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg
     1201 ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag
     1261 aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc
     1321 taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc
     1381 agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg
     1441 aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg
     1501 gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag
     1561 cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
     1621 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
     1681 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
     1741 ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc gtcggaaaag
     1801 ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc gtatgtaagg
     1861 atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc
     1921 ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat
     1981 tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
     2041 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     2101 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
     2161 aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
     2221 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
     2281 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
     2341 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
     2401 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
     2461 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
     2521 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
     2581 cttagacagc cg
//
LOCUS       DAFEZB010000290         2340 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.169, whole genome shotgun sequence.
ACCESSION   DAFEZB010000290
VERSION     DAFEZB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.169"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            192..1232
                     /locus_tag="LP859_003052"
     CDS             192..1232
                     /locus_tag="LP859_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBL3030898.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            1330..>2340
                     /gene="tet"
                     /locus_tag="LP859_003053"
     CDS             1330..>2340
                     /gene="tet"
                     /locus_tag="LP859_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HBL3030899.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFL"
BASE COUNT          728 a          347 c          468 g          797 t
ORIGIN      
        1 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
       61 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
      121 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
      181 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
      241 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
      301 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
      361 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
      421 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
      481 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
      541 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
      601 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
      661 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
      721 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
      781 atggttggaa tgtgacggat tgtaaaatct gttttaagta tggcttatac tatagccctg
      841 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
      901 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
      961 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     1021 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     1081 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     1141 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     1201 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     1261 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     1321 gaattaatta tgaagtgtaa tgaatgtaac agggttaaat acaaccaaat tttaatttgg
     1381 ctttgcgttt tatctttttt tagcgtatta aatgaaatgg ttttgaacgt ctcattacct
     1441 gatattgcaa atgattttaa taaaccacct gcgagtacaa actgggtgaa cacagccttt
     1501 atgttaacct tttccattgg aacagctgta tatggaaagc tatctgatca attaggcatc
     1561 aaaaggttac tcctatttgg aattataata aattgtttcg ggtcggtaat tgggtttgtt
     1621 ggccattctt tcttttcctt acttattatg gctcgtttta ttcaaggggc tggtgcagct
     1681 gcatttccag cactcgtaat ggttgtagtt gcgcgctata ttccaaagga aaataggggt
     1741 aaagcatttg gtcttattgg atcgatagta gccatgggag aaggagtcgg tccagcgatt
     1801 ggtggaatga tagcccatta tattcattgg tcctatcttc tactcattcc tatgataaca
     1861 attatcactg ttccgtttct tatgaaatta ttaaagaaag aagtaaggat aaaaggtcat
     1921 tttgatatca aaggaattat actaatgtct gtaggcattg tattttttat gttgtttaca
     1981 acatcatata gcatttcttt tcttatcgtt agcgtgctgt cattcctgat atttgtaaaa
     2041 catatcagga aagtaacaga tccttttgtt gatcccggat tagggaaaaa tatacctttt
     2101 atgattggag ttctttgtgg gggaattata tttggaacag tagcagggtt tgtctctatg
     2161 gttccttata tgatgaaaga tgttcaccag ctaagtactg ccgaaatcgg aagtgtaatt
     2221 attttccctg gaacaatgag tgtcattatt ttcggctaca ttggtgggat acttgttgat
     2281 agaagaggtc ctttatacgt gttaaacatc ggagttacat ttctttctgt tagcttttta
//
LOCUS       DAFEZB010000291         2277 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.171, whole genome shotgun sequence.
ACCESSION   DAFEZB010000291
VERSION     DAFEZB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.171"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..788
                     /locus_tag="LP859_003054"
     CDS             <1..788
                     /locus_tag="LP859_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBL3030900.1"
                     /translation="DYDITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEK
                     GFSYLMLFDDYNKIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVR
                     KPSAREYDDCCNEFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETG
                     FKLSVGKNYKFIERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERL
                     HYAYPEYDRNITKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            785..1327
                     /gene="sat4"
                     /locus_tag="LP859_003055"
     CDS             785..1327
                     /gene="sat4"
                     /locus_tag="LP859_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3030901.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1420..2214
                     /locus_tag="LP859_003056"
                     /note="aph(3')-IIIa"
     CDS             1420..2214
                     /locus_tag="LP859_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL3030902.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          762 a          355 c          538 g          622 t
ORIGIN      
        1 aggattatga tattacatat tttgtaagtg atatagaacc gtttatatct aatgatgact
       61 ggcttaatca atttgggaat ataataatga tgcaaaagcc ggaggatatg gaattattcc
      121 cacctgaaga aaagggattt tcctatctta tgctatttga tgattacaat aaaattgatc
      181 ttaccttatt gcccttggaa gagttagata attacctaaa gggcgataaa ttaataaagg
      241 ttctaattga taaagattgt agaattaaaa gggacatagt tccgactgat atagattatc
      301 atgtaagaaa gccaagcgca agggagtatg atgattgctg caatgaattt tggaatgtaa
      361 caccttatgt tattaaagga ttgtgccgta aggaaatttt atttgctatt gatcatttta
      421 atcagattgt tcgccatgag ctgctgagaa tgatatcatg gaaggtcggc atcgaaacag
      481 gctttaaatt aagtgtaggc aagaactata agtttattga aaggtatata tccgaggatt
      541 tgtgggagaa acttttgtcc acctaccgga tggattccta tgaaaacata tgggaagcat
      601 tatttctatg ccatcaattg ttcagggcgg tatccggtga ggtggcggaa aggcttcatt
      661 atgcctatcc ggagtatgat aggaatataa caaaatatac cagggacatg tataaaaaat
      721 acactggtaa aaccggctgc ctggatagca catatgccgc tgatatagaa gagaggcggg
      781 aacagtgatt acagaaatga aagcagggca cctgaaagat atcgataaac ccagcgaacc
      841 atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga cctttacaga
      901 attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg aagaggatga
      961 ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc tttactacca
     1021 agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat tggaaccggt acgcttatat
     1081 agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa
     1141 tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga
     1201 caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga
     1261 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
     1321 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
     1381 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
     1441 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     1501 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     1561 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
     1621 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
     1681 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
     1741 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
     1801 tcggattgtc cctatacgaa tagcttagac agccgattag ccgaattgga ttacttactg
     1861 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
     1921 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
     1981 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
     2041 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
     2101 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
     2161 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
     2221 gatttagatg tctaaaaagc tttaactaca agctttttag acatctaatc ttttctg
//
LOCUS       DAFEZB010000292         2277 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.172, whole genome shotgun sequence.
ACCESSION   DAFEZB010000292
VERSION     DAFEZB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.172"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..788
                     /locus_tag="LP859_003057"
     CDS             <1..788
                     /locus_tag="LP859_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBL3030903.1"
                     /translation="DYDITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEK
                     GFSYLMLFDDYNKIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVR
                     KPSAREYDDCCNEFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETG
                     FKLSVGKNYKFIERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERL
                     HYAYPEYDRNITKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            785..1327
                     /gene="sat4"
                     /locus_tag="LP859_003058"
     CDS             785..1327
                     /gene="sat4"
                     /locus_tag="LP859_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3030904.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1420..2214
                     /locus_tag="LP859_003059"
                     /note="aph(3')-IIIa"
     CDS             1420..2214
                     /locus_tag="LP859_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL3030905.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          761 a          356 c          538 g          622 t
ORIGIN      
        1 aggattatga tattacatat tttgtaagtg atatagaacc gtttatatct aatgatgact
       61 ggcttaatca atttgggaat ataataatga tgcaaaagcc ggaggatatg gaattattcc
      121 cacctgaaga aaagggattt tcctatctta tgctatttga tgattacaat aaaattgatc
      181 ttaccttatt gcccttggaa gagttagata attacctaaa gggcgataaa ttaataaagg
      241 ttctaattga taaagattgt agaattaaaa gggacatagt tccgactgat atagattatc
      301 atgtaagaaa gccaagcgca agggagtatg atgattgctg caatgaattt tggaatgtaa
      361 caccttatgt tattaaagga ttgtgccgta aggaaatttt atttgctatt gatcatttta
      421 atcagattgt tcgccatgag ctgctgagaa tgatatcatg gaaggtcggc atcgaaacag
      481 gctttaaatt aagtgtaggc aagaactata agtttattga aaggtatata tccgaggatt
      541 tgtgggagaa acttttgtcc acctaccgga tggattccta tgaaaacata tgggaagcat
      601 tatttctatg ccatcaattg ttcagggcgg tatccggtga ggtggcggaa aggcttcatt
      661 atgcctatcc ggagtatgat aggaatataa caaaatatac cagggacatg tataaaaaat
      721 acactggtaa aaccggctgc ctggatagca catatgccgc tgatatagaa gagaggcggg
      781 aacagtgatt acagaaatga aagcagggca cctgaaagat atcgataaac ccagcgaacc
      841 atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga cctttacaga
      901 attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg aagaggatga
      961 ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc tttactacca
     1021 agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat tggaaccggt acgcttatat
     1081 agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa
     1141 tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga
     1201 caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga
     1261 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
     1321 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
     1381 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
     1441 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     1501 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     1561 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
     1621 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
     1681 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
     1741 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
     1801 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
     1861 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
     1921 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
     1981 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
     2041 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
     2101 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
     2161 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
     2221 gatttagatg tctaaaaagc tttaactaca agctttttag acatctaatc ttttctg
//
LOCUS       DAFEZB010000293         1860 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.181, whole genome shotgun sequence.
ACCESSION   DAFEZB010000293
VERSION     DAFEZB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.181"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            22..933
                     /locus_tag="LP859_003060"
     CDS             22..933
                     /locus_tag="LP859_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL3030906.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1086..1571
                     /gene="vanZ"
                     /locus_tag="LP859_003061"
     CDS             1086..1571
                     /gene="vanZ"
                     /locus_tag="LP859_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL3030907.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          656 a          257 c          355 g          592 t
ORIGIN      
        1 tagttaagga ggagactgaa aatgaagaag ttgttttttt tattgttatt gttattctta
       61 atatacttag gttatgacta cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt
      121 caaaattatg atcaaaatcc caaagaacat ttagaaaata gtgggacttc tgaaaatacc
      181 caagagaaaa caattacaga agaacaggtt tatcaaggaa atctgctatt aatcaatagt
      241 aaatatcctg ttcgccaaga aagtgtgaag tcagatatcg tgaatttatc taaacatgac
      301 gaattaataa atggatacgg gttgcttgat agtaatattt atatgtcaaa agaaatagca
      361 caaaaatttt cagagatggt caatgatgct gtaaagggtg gcgttagtca ttttattatt
      421 aatagtggct atcgagactt tgatgagcaa agtgtgcttt accaagaaat gggggctgag
      481 tatgccttac cagcaggtta tagtgagcat aattcaggtt tatcactaga tgtaggatca
      541 agcttgacga aaatggaacg agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa
      601 tacgggttca ttttacgtta tccagaggac aaaacagagt taacaggaat tcaatatgaa
      661 ccatggcata ttcgctatgt tggtttacca catagtgcga ttatgaaaga aaagaatttc
      721 gttctcgagg aatatatgga ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat
      781 ggggaaaaat atgagatctt ttattatcct gttactaaaa ataccaccat tcatgtgccg
      841 actaatcttc gttatgagat atcaggaaac aatatagacg gtgtaattgt gacagtgttt
      901 cccggatcaa cacatactaa ttcaaggagg taaggatggc ggaatgaaac caacgaaatt
      961 aatgaacagc attattgtac tagcactttt ggggtaacgt tagcttttta atttaaaacc
     1021 cacgttaact aggacattgc tatactaatg atacaactta aacaaaagaa ttagaggaaa
     1081 ttatattggg aaaaatatta tctagaggat tgctagcttt atatttagtg acactaatct
     1141 ggttagtgtt attcaaatta caatacaata ttttatcagt atttaattat catcaaagaa
     1201 gtcttaactt gactccattt actgctactg ggaatttcag agagatgata gataatgtta
     1261 taatctttat tccatttggc ttgcttttga atgtcaattt taaagaaatc ggatttttac
     1321 ctaagtttgc ttttgtactg gttttaagtc ttacttttga aataattcaa tttatcttcg
     1381 ctattggagc gacagacata acagatgtaa ttacaaatac tgttggaggc tttcttggac
     1441 tgaaattata tggtttaagc aataagcata tgaatcaaaa aaaattagac agagttatta
     1501 tttttgtagg tatacttttg ctcgtattat tgctcgttta ccgtacccat ttaagaataa
     1561 attacgtgta agatgtctaa atcaagcaat ctgatctttc atacacataa agatattgaa
     1621 tgaattggat tagatggaaa acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg
     1681 ggaaaaccgg tgataaagta aaaagcttac ctaacactat agtaacaaag aaagcccaat
     1741 tatcaatttt agtgctgagg aattggtctc tttaataaat ttccttaacg ttgtaaatcc
     1801 gcattttcct gacggtaccc ccaaactaag atttcataga tagattttct gataacttta
//
LOCUS       DAFEZB010000294         1699 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.185, whole genome shotgun sequence.
ACCESSION   DAFEZB010000294
VERSION     DAFEZB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.185"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..976
                     /gene="vanA"
                     /locus_tag="LP859_003062"
     CDS             <1..976
                     /gene="vanA"
                     /locus_tag="LP859_003062"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HBL3030908.1"
                     /translation="SVKSAIEIAANINKEKYEPLYIGITKSGVWKMCEKPCAEWENDN
                     CYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKSGEDGSIQGLFELSGIPFVG
                     CDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPVAATFTYPVFVKPARSGSSF
                     GVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQ
                     YGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFL
                     QDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            982..1590
                     /locus_tag="LP859_003063"
     CDS             982..1590
                     /locus_tag="LP859_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL3030909.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          505 a          328 c          417 g          449 t
ORIGIN      
        1 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
       61 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
      121 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
      181 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
      241 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
      301 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
      361 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
      421 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
      481 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
      541 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
      601 attgggaaac agtgccgcgt tagctgttgg cgaggtggac caaatcaggc tgcagtacgg
      661 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
      721 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
      781 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
      841 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
      901 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
      961 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     1021 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     1081 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     1141 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaat
     1201 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     1261 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     1321 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
     1381 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
     1441 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
     1501 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
     1561 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat
     1621 aagctaactc tttcggcagg aaacccgacg tatgtaactg gttcttaggg aatttatata
     1681 tagtagatag tattgaaga
//
LOCUS       DAFEZB010000295         1417 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00358_1
            SAMN20572080-rid13345273.guided.194, whole genome shotgun sequence.
ACCESSION   DAFEZB010000295
VERSION     DAFEZB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572080
            Sequence Read Archive: SRR16866804
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176639.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572080-rid13345273.guided.194"
                     /isolate="BL00358_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2013-09"
                     /collected_by="University of Michigan"
     gene            <1..1348
                     /locus_tag="LP859_003064"
     CDS             <1..1348
                     /locus_tag="LP859_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBL3030910.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          604 a          109 c          235 g          469 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataat
//
